AT1G05820

Signal peptide peptidase-like 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005706
Physical Location & Seq
Forward (+)
1749478 .. 1754036
4559 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G05820.4

Sequence Viewer

Length: 1611 bp
ATGTCTTTACCTCCGTTTACTTGCCGCCTTCTCGCGGCGGCGGCGGCTCTCTATCTGATAGGTCTGCTGTGCGTCGGAGCTGACACGAAAGATGTTACCGCTCCCAAAATTCCTGGTTGCTCCAATGAATTTCAAATGGTCAAAGTTGAGAATTGGGTCAACGGAGAAAACGGTGAGACTTTTACCGCCATGACTGCACAGTTTGGCACCATGCTCCCGTCTGATAAAGACAAAGCTGTTAAACTTCCGGTTGCTCTTACCACTCCTTTGGACAGTTGCTCCAATTTAACTTCAAAGCTATCTTGGTCTATAGCGTTATCTGTACGTGGCGAGTGTGCTTTCACAGTTAAGGCTCAAGTTGCACAGGCAGGAGGAGCTGCAGCTTTGGTGTTAATTAACGACAAAGAAGAGCTTGATGAGATGGTTTGTGGTGAGAAAGATACCTCCTTAAATGTTTCTATACCTATTTTGATGATTACAACGTCATCTGGAGATGCCCTGAAGAAATCCATTATGCAAAATAAGAAAGTGGAGCTTTTATTGTATGCTCCAAAGAGCCCAATTGTGGATTATGCAGTGGTCTTCCTCTGGCTAATGTCTGTTGGAACAGTTTTTGTTGCTTCTGTTTGGTCACATGTTACCAGTCCGAAGAAGAATGATGAGCAGTACGATGAATTATCACCCAAGAAATCTTCGAATGTTGACGCTACCAAAGGTGGTGCTGAAGAGGAAACTCTTGATATTAGTGCTATGGGTGCTGTTATCTTTGTCATATCAGCGTCCACATTCCTCGTTTTGCTCTTCTTCTTCATGTCATCGTGGTTTATCTTGATCCTGACCATATTTTTCGTCATTGGTGGTATGCAGGGAATGCATAATATTAACGTTACACTCATAACAAGGAGATGCAGTAAATGTGGCCAGAAGAATTTAAAACTACCTCTGCTTGGGAATACCTCGATTCTCTCACTCGTGGTTCTGTTATTCTGCTTTGTGGTTGCTATCCTCTGGTTTATGAATCGCAAAACTTCGCACGCATGGGCCGGGCAAGATATTTTTGGTATTTGCATGATGATTAATGTCTTGCAAGTAGCTCGGCTACCTAATATCAGGGTTGCTACCATTCTTCTCTGTTGTGCATTTTTCTATGACATCTTTTGGGTATTCATATCACCACTAATCTTCAAGCAAAGTGTAATGATTGCGGTTGCACGTGGGAGCAAAGACACTGGAGAATCTATTCCCATGCTTTTGAGAATTCCACGGCTTTCTGATCCATGGGGTGGTTACAACATGATCGGTTTTGGAGACATTCTTTTCCCGGGCCTTCTCATATGCTTTATTTTCAGATTTGACAAGGAAAATAACAAGGGGGTCTCGAATGGATATTTTCCGTGGTTGATGTTTGGCTACGGACTTGGCCTCTTCTTAACATACTTGGGATTGTATGTTATGAATGGACACGGTCAACCTGCATTGCTATATCTTGTCCCTTGCACGCTCGGAATCACGGTCATTCTAGGGTTGGTGAGGAAAGAACTCAGAGACTTGTGGAACTACGGGACTCAACAGCCTTCAGCTGCAGACGTAAATCCATCTCCAGAAGCATAA

Protein Analysis

536

Amino Acids

58.73

Weight (kDa)

7.41

Isoelectric Point (pI)

34.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_A22B PF04258 102 - 383 1.4e-104 Signal peptide peptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1480
AccB1I GGYRCC 1 cut(s) 206
AccB7I CCANNNNNTGG 1 cut(s) 1283
AccBSI CCGCTC 1 cut(s) 101
AccII CGCG 1 cut(s) 35
AciI CCGC 8 cut(s) 25, 35, 38, 41, 44, 99, 186, 1205
AclI AACGTT 1 cut(s) 885
AclWI GGATC 2 cut(s) 826, 1268
AcoI YGGCCR 1 cut(s) 919
AcsI RAATTY 4 cut(s) 108, 128, 928, 1257
AcuI CTGAAG 3 cut(s) 521, 744, 1560
AcvI CACGTG 1 cut(s) 1214
AfaI GTAC 2 cut(s) 324, 668
AfiI CCNNNNNNNGG 4 cut(s) 34, 565, 947, 1283
AflIII ACRYGT 1 cut(s) 634
AgsI TTSAA 3 cut(s) 134, 294, 1186
AjnI CCWGG 1 cut(s) 112
AluBI AGCT 9 cut(s) 80, 236, 298, 377, 383, 412, 535, 1094, 1580
AluI AGCT 9 cut(s) 80, 236, 298, 377, 383, 412, 535, 1094, 1580
Alw26I GTCTC 4 cut(s) 170, 1302, 1381, 1539
AlwI GGATC 2 cut(s) 826, 1268
Ama87I CYCGRG 1 cut(s) 1321
AoxI GGCC 4 cut(s) 919, 1041, 1324, 1420
ApeKI GCWGC 3 cut(s) 377, 380, 1580
ApoI RAATTY 4 cut(s) 108, 128, 928, 1257
AseI ATTAAT 1 cut(s) 1077
Asp700I GAANNNNTTC 1 cut(s) 1239
AspS9I GGNCC 2 cut(s) 1041, 1324
AsuC2I CCSGG 3 cut(s) 1045, 1322, 1323
AsuHPI GGTGA 5 cut(s) 185, 443, 672, 1164, 1540
AsuII TTCGAA 1 cut(s) 695
AvaI CYCGRG 1 cut(s) 1321
BalI TGGCCA 1 cut(s) 921
BanI GGYRCC 1 cut(s) 206
BanII GRGCYC 1 cut(s) 560
BauI CACGAG 1 cut(s) 971
BbrPI CACGTG 1 cut(s) 1214
BbsI GAAGAC 1 cut(s) 574
BbvI GCAGC 3 cut(s) 364, 392, 1567
BccI CCATC 2 cut(s) 415, 1603
BceAI ACGGC 1 cut(s) 1280
BcgI CGANNNNNNTGC 2 cut(s) 1076, 1110
BciT130I CCWGG 1 cut(s) 114
BcnI CCSGG 3 cut(s) 1045, 1322, 1323
BcoDI GTCTC 4 cut(s) 170, 1302, 1381, 1539
BfaI CTAG 1 cut(s) 1520
BfmI CTRYAG 3 cut(s) 309, 378, 1581
BfuAI ACCTGC 1 cut(s) 1480
BisI GCNGC 8 cut(s) 25, 36, 39, 42, 45, 378, 381, 1581
BlsI GCNGC 8 cut(s) 26, 37, 40, 43, 46, 379, 382, 1582
Bme1390I CCNGG 4 cut(s) 114, 1045, 1322, 1323
BmeT110I CYCGRG 1 cut(s) 1321
BmgT120I GGNCC 2 cut(s) 1041, 1324
BmiI GGNNCC 1 cut(s) 208
BmrFI CCNGG 4 cut(s) 114, 1045, 1322, 1323
BmsI GCATC 2 cut(s) 484, 896
BpiI GAAGAC 1 cut(s) 574
BpmI CTGGAG 3 cut(s) 510, 1251, 1584
Bpu14I TTCGAA 1 cut(s) 695
BpuEI CTTGAG 1 cut(s) 339
BpuMI CCSGG 3 cut(s) 1045, 1322, 1323
BsaAI YACGTR 2 cut(s) 326, 1214
BsaI GGTCTC 1 cut(s) 1381
BsaJI CCNNGG 4 cut(s) 1262, 1277, 1321, 1394
BsaWI WCCGGW 1 cut(s) 247
BsaXI ACNNNNNCTCC 4 cut(s) 263, 293, 895, 925
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 565, 947, 1283
Bse1I ACTGG 2 cut(s) 642, 1234
Bse3DI GCAATG 1 cut(s) 1475
BseBI CCWGG 1 cut(s) 114
BseDI CCNNGG 4 cut(s) 1262, 1277, 1321, 1394
BseLI CCNNNNNNNGG 4 cut(s) 34, 565, 947, 1283
BseMI GCAATG 1 cut(s) 1475
BseMII CTCAG 1 cut(s) 1555
BseNI ACTGG 2 cut(s) 642, 1234
BseRI GAGGAG 1 cut(s) 387
BseXI GCAGC 3 cut(s) 364, 392, 1567
BsgI GTGCAG 1 cut(s) 180
Bsh1236I CGCG 1 cut(s) 35
BshFI GGCC 4 cut(s) 921, 1043, 1326, 1422
BshNI GGYRCC 1 cut(s) 206
BsiHKCI CYCGRG 1 cut(s) 1321
BsiSI CCGG 3 cut(s) 248, 1044, 1322
BslFI GGGAC 2 cut(s) 1475, 1576
BslI CCNNNNNNNGG 4 cut(s) 34, 565, 947, 1283
BsmAI GTCTC 4 cut(s) 170, 1302, 1381, 1539
BsmFI GGGAC 2 cut(s) 1475, 1576
BsmI GAATGC 1 cut(s) 876
BsnI GGCC 4 cut(s) 921, 1043, 1326, 1422
Bso31I GGTCTC 1 cut(s) 1381
BsoBI CYCGRG 1 cut(s) 1321
Bsp119I TTCGAA 1 cut(s) 695
Bsp1286I GDGCHC 1 cut(s) 560
Bsp143I GATC 3 cut(s) 831, 1273, 1296
Bsp19I CCATGG 1 cut(s) 1277
BspACI CCGC 8 cut(s) 25, 35, 38, 41, 44, 99, 186, 1205
BspANI GGCC 4 cut(s) 921, 1043, 1326, 1422
BspCNI CTCAG 1 cut(s) 1554
BspFNI CGCG 1 cut(s) 35
BspLI GGNNCC 1 cut(s) 208
BspMAI CTGCAG 2 cut(s) 382, 1585
BspMI ACCTGC 1 cut(s) 1480
BspPI GGATC 2 cut(s) 826, 1268
BspQI GCTCTTC 2 cut(s) 402, 806
BspT104I TTCGAA 1 cut(s) 695
BspT107I GGYRCC 1 cut(s) 206
BspTNI GGTCTC 1 cut(s) 1381
BsrBI CCGCTC 1 cut(s) 101
BsrDI GCAATG 1 cut(s) 1475
BsrI ACTGG 2 cut(s) 642, 1234
BssECI CCNNGG 4 cut(s) 1262, 1277, 1321, 1394
BssMI GATC 3 cut(s) 831, 1273, 1296
BssSI CACGAG 1 cut(s) 971
BssT1I CCWWGG 1 cut(s) 1277
Bst2BI CACGAG 1 cut(s) 971
Bst2UI CCWGG 1 cut(s) 114
Bst4CI ACNGT 7 cut(s) 173, 201, 275, 346, 610, 1466, 1513
Bst6I CTCTTC 4 cut(s) 402, 720, 806, 1430
BstAPI GCANNNNNTGC 1 cut(s) 871
BstBAI YACGTR 2 cut(s) 326, 1214
BstBI TTCGAA 1 cut(s) 695
BstC8I GCNNGC 2 cut(s) 1035, 1499
BstDEI CTNAG 1 cut(s) 1541
BstDSI CCRYGG 3 cut(s) 1262, 1277, 1394
BstFNI CGCG 1 cut(s) 35
BstKTI GATC 3 cut(s) 834, 1276, 1299
BstMAI GTCTC 4 cut(s) 170, 1302, 1381, 1539
BstMBI GATC 3 cut(s) 831, 1273, 1296
BstMWI GCNNNNNNNGC 7 cut(s) 41, 44, 194, 359, 374, 755, 871
BstNI CCWGG 1 cut(s) 114
BstNSI RCATGY 1 cut(s) 638
BstSCI CCNGG 4 cut(s) 112, 1043, 1320, 1321
BstSFI CTRYAG 3 cut(s) 309, 378, 1581
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 3 cut(s) 364, 392, 1567
BstV2I GAAGAC 1 cut(s) 574
BstXI CCANNNNNNTGG 1 cut(s) 268
BsuRI GGCC 4 cut(s) 921, 1043, 1326, 1422
BtgI CCRYGG 3 cut(s) 1262, 1277, 1394
BtsI GCAGTG 1 cut(s) 582
BtsIMutI CAGTG 2 cut(s) 582, 1227
BveI ACCTGC 1 cut(s) 1480
Cac8I GCNNGC 2 cut(s) 1035, 1499
Cfr13I GGNCC 2 cut(s) 1041, 1324
Cfr9I CCCGGG 1 cut(s) 1321
CseI GACGC 3 cut(s) 61, 713, 768
Csp6I GTAC 2 cut(s) 323, 667
CviAII CATG 9 cut(s) 190, 211, 635, 811, 1038, 1069, 1246, 1278, 1294
CviQI GTAC 2 cut(s) 323, 667
DdeI CTNAG 1 cut(s) 1541
DpnI GATC 3 cut(s) 833, 1275, 1298
DpnII GATC 3 cut(s) 831, 1273, 1296
DraI TTTAAA 1 cut(s) 933
EaeI YGGCCR 1 cut(s) 919
Eam1104I CTCTTC 4 cut(s) 402, 720, 806, 1430
EarI CTCTTC 4 cut(s) 402, 720, 806, 1430
Eco130I CCWWGG 1 cut(s) 1277
Eco24I GRGCYC 1 cut(s) 560
Eco31I GGTCTC 1 cut(s) 1381
Eco57I CTGAAG 3 cut(s) 521, 744, 1560
Eco72I CACGTG 1 cut(s) 1214
Eco88I CYCGRG 1 cut(s) 1321
EcoRI GAATTC 1 cut(s) 1257
EcoRII CCWGG 1 cut(s) 112
EcoT14I CCWWGG 1 cut(s) 1277
EcoT22I ATGCAT 1 cut(s) 876
EcoT38I GRGCYC 1 cut(s) 560
ErhI CCWWGG 1 cut(s) 1277
FaeI CATG 9 cut(s) 193, 214, 638, 814, 1041, 1072, 1249, 1281, 1297
FalI AAGNNNNNCTT 4 cut(s) 396, 428, 519, 551
FaqI GGGAC 2 cut(s) 1475, 1576
FatI CATG 9 cut(s) 189, 210, 634, 810, 1037, 1068, 1245, 1277, 1293
FauNDI CATATG 1 cut(s) 1334
Fnu4HI GCNGC 8 cut(s) 25, 36, 39, 42, 45, 378, 381, 1581
FriOI GRGCYC 1 cut(s) 560
Fsp4HI GCNGC 8 cut(s) 25, 36, 39, 42, 45, 378, 381, 1581
FspBI CTAG 1 cut(s) 1520
GluI GCNGC 8 cut(s) 25, 36, 39, 42, 45, 378, 381, 1581
GsuI CTGGAG 3 cut(s) 510, 1251, 1584
HaeIII GGCC 4 cut(s) 921, 1043, 1326, 1422
HapII CCGG 3 cut(s) 248, 1044, 1322
HgaI GACGC 3 cut(s) 61, 713, 768
Hin1II CATG 9 cut(s) 193, 214, 638, 814, 1041, 1072, 1249, 1281, 1297
HincII GTYRAC 3 cut(s) 160, 703, 1469
HindII GTYRAC 3 cut(s) 160, 703, 1469
HinfI GANTC 5 cut(s) 961, 1018, 1235, 1506, 1564
HpaII CCGG 3 cut(s) 248, 1044, 1322
HphI GGTGA 5 cut(s) 185, 443, 672, 1164, 1540
Hpy166II GTNNAC 5 cut(s) 18, 160, 703, 783, 1469
Hpy188I TCNGA 8 cut(s) 57, 77, 223, 648, 1273, 1349, 1505, 1544
Hpy188III TCNNGA 6 cut(s) 489, 737, 829, 835, 1378, 1601
Hpy8I GTNNAC 5 cut(s) 18, 160, 703, 783, 1469
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 3 cut(s) 38, 1337, 1584
HpyCH4III ACNGT 7 cut(s) 173, 201, 275, 346, 610, 1466, 1513
HpyCH4IV ACGT 5 cut(s) 325, 482, 885, 1213, 1587
HpyF10VI GCNNNNNNNGC 7 cut(s) 41, 44, 194, 359, 374, 755, 871
HpyF3I CTNAG 1 cut(s) 1541
HpySE526I ACGT 5 cut(s) 325, 482, 885, 1213, 1587
Hsp92II CATG 9 cut(s) 193, 214, 638, 814, 1041, 1072, 1249, 1281, 1297
Kzo9I GATC 3 cut(s) 831, 1273, 1296
LguI GCTCTTC 2 cut(s) 402, 806
LmnI GCTCC 9 cut(s) 77, 106, 125, 219, 284, 374, 532, 553, 1218
Lsp1109I GCAGC 3 cut(s) 364, 392, 1567
LweI GCATC 2 cut(s) 484, 896
MaeI CTAG 1 cut(s) 1520
MaeII ACGT 5 cut(s) 325, 482, 885, 1213, 1587
MaeIII GTNAC 5 cut(s) 94, 630, 637, 886, 1286
MalI GATC 3 cut(s) 833, 1275, 1298
MbiI CCGCTC 1 cut(s) 101
MboI GATC 3 cut(s) 831, 1273, 1296
MfeI CAATTG 1 cut(s) 561
MhlI GDGCHC 1 cut(s) 560
MlsI TGGCCA 1 cut(s) 921
MluCI AATT 9 cut(s) 108, 128, 151, 283, 393, 561, 674, 928, 1257
MluNI TGGCCA 1 cut(s) 921
MlyI GAGTC 1 cut(s) 1558
MmeI TCCRAC 2 cut(s) 55, 583
Mox20I TGGCCA 1 cut(s) 921
Mph1103I ATGCAT 1 cut(s) 876
MroXI GAANNNNTTC 1 cut(s) 1239
MscI TGGCCA 1 cut(s) 921
Msp20I TGGCCA 1 cut(s) 921
MspA1I CMGCKG 1 cut(s) 1580
MspI CCGG 3 cut(s) 248, 1044, 1322
MspR9I CCNGG 4 cut(s) 114, 1045, 1322, 1323
MunI CAATTG 1 cut(s) 561
Mva1269I GAATGC 1 cut(s) 876
MvaI CCWGG 1 cut(s) 114
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 7 cut(s) 41, 44, 194, 359, 374, 755, 871
NciI CCSGG 3 cut(s) 1045, 1322, 1323
NcoI CCATGG 1 cut(s) 1277
NdeI CATATG 1 cut(s) 1334
NdeII GATC 3 cut(s) 831, 1273, 1296
NlaIII CATG 9 cut(s) 193, 214, 638, 814, 1041, 1072, 1249, 1281, 1297
NlaIV GGNNCC 1 cut(s) 208
NmeAIII GCCGAG 1 cut(s) 1075
NmuCI GTSAC 1 cut(s) 630
NsiI ATGCAT 1 cut(s) 876
NspI RCATGY 1 cut(s) 638
NspV TTCGAA 1 cut(s) 695
PacI TTAATTAA 1 cut(s) 396
PciI ACATGT 1 cut(s) 634
PciSI GCTCTTC 2 cut(s) 402, 806
PctI GAATGC 1 cut(s) 876
PdmI GAANNNNTTC 1 cut(s) 1239
PfeI GAWTC 4 cut(s) 961, 1018, 1235, 1506
PflFI GACNNNGTC 1 cut(s) 1464
PflMI CCANNNNNTGG 1 cut(s) 1283
PkrI GCNGC 8 cut(s) 26, 37, 40, 43, 46, 379, 382, 1582
PleI GAGTC 1 cut(s) 1558
PmaCI CACGTG 1 cut(s) 1214
PmlI CACGTG 1 cut(s) 1214
PpsI GAGTC 1 cut(s) 1558
Ppu21I YACGTR 2 cut(s) 326, 1214
PscI ACATGT 1 cut(s) 634
PshBI ATTAAT 1 cut(s) 1077
Psp1406I AACGTT 1 cut(s) 885
Psp6I CCWGG 1 cut(s) 112
PspCI CACGTG 1 cut(s) 1214
PspGI CCWGG 1 cut(s) 112
PspN4I GGNNCC 1 cut(s) 208
PspPI GGNCC 2 cut(s) 1041, 1324
PstI CTGCAG 2 cut(s) 382, 1585
PsyI GACNNNGTC 1 cut(s) 1464
PvuII CAGCTG 1 cut(s) 1580
RsaI GTAC 2 cut(s) 324, 668
RsaNI GTAC 2 cut(s) 323, 667
SapI GCTCTTC 2 cut(s) 402, 806
SatI GCNGC 8 cut(s) 25, 36, 39, 42, 45, 378, 381, 1581
Sau3AI GATC 3 cut(s) 831, 1273, 1296
Sau96I GGNCC 2 cut(s) 1041, 1324
SchI GAGTC 1 cut(s) 1558
ScrFI CCNGG 4 cut(s) 114, 1045, 1322, 1323
SduI GDGCHC 1 cut(s) 560
SfaNI GCATC 2 cut(s) 484, 896
SfcI CTRYAG 3 cut(s) 309, 378, 1581
SfuI TTCGAA 1 cut(s) 695
SmaI CCCGGG 1 cut(s) 1323
SmlI CTYRAG 1 cut(s) 354
SmoI CTYRAG 1 cut(s) 354
Sse9I AATT 9 cut(s) 108, 128, 151, 283, 393, 561, 674, 928, 1257
SsiI CCGC 8 cut(s) 25, 35, 38, 41, 44, 99, 186, 1205
SspI AATATT 1 cut(s) 880
SspMI CTAG 1 cut(s) 1520
StyD4I CCNGG 4 cut(s) 112, 1043, 1320, 1321
StyI CCWWGG 1 cut(s) 1277
TaaI ACNGT 7 cut(s) 173, 201, 275, 346, 610, 1466, 1513
TaiI ACGT 5 cut(s) 328, 485, 888, 1216, 1590
TaqI TCGA 3 cut(s) 695, 959, 1379
TasI AATT 9 cut(s) 108, 128, 151, 283, 393, 561, 674, 928, 1257
TauI GCSGC 5 cut(s) 27, 38, 41, 44, 47
TfiI GAWTC 4 cut(s) 961, 1018, 1235, 1506
TscAI CASTG 2 cut(s) 582, 1234
TseFI GTSAC 1 cut(s) 630
TseI GCWGC 3 cut(s) 377, 380, 1580
Tsp45I GTSAC 1 cut(s) 630
TspDTI ATGAA 6 cut(s) 141, 687, 799, 1031, 1156, 1469
TspGWI ACGGA 3 cut(s) 177, 1383, 1428
TspMI CCCGGG 1 cut(s) 1321
TspRI CASTG 2 cut(s) 582, 1234
Tth111I GACNNNGTC 1 cut(s) 1464
Van91I CCANNNNNTGG 1 cut(s) 1283
VspI ATTAAT 1 cut(s) 1077
XapI RAATTY 4 cut(s) 108, 128, 928, 1257
XceI RCATGY 1 cut(s) 638
XmaI CCCGGG 1 cut(s) 1321
XmnI GAANNNNTTC 1 cut(s) 1239
XspI CTAG 1 cut(s) 1520
Zsp2I ATGCAT 1 cut(s) 876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.