Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0005706

Physical Location & Seq

Genomic Coordinates Forward (+)

38772469 .. 38780726

Gene Structure 8258 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1278000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1617 bp

ATGGCAATGGCGTCGGGTCTCCTCCGTCTTGTGTTTCTGTTATCTCTGATTGCTGTGTCTTTAGCCCGAGGTGAAAACGATATGGTTTTGGATGTCGATTCCACCCCCAGCACTCCTTCCTGCAACAACCCGTACCTAATGGCAAAGGTTAAGAATTGGGTTAATGGCCGTAAAGGCGAAACTATTCAAGGGGCAGGTGCAAAGTTTGGGGCTTTATTGCCTTCCAAAGAAGAAAAAGCCGTCAGATTGCCCGTTGTTATCTCGAATCCCTTAAATGGTTGTTCTGTGTCGTCTTCGAAGTTATTTGGAGCTATTGCCTTGTCCACGCGTGGTGATTGTGACTTCTCCGTCAAGGCAGAAGTTGCGCAGTCAGGAGGTGCTAAAGCGCTGCTGGTATTAAATAATGAAGAAGAGCTTGCCAAGATGGGCTGTCCTGATAATAGCACTTCTTTGAATATCTCAATTTTTGTTGTAATGGTTCCAAAATCAGATGGAGAAGCTCTCAAGAAATCTATTGAAGATGGAAAGAAAGTGGAGCTTCTATTATATTCTCCCAAGCGTCCAGTGGTGGACTACTCAGTTATGTTCTTGTGGCTGATGGCTATTGGAACAATAATAATTGCTTCATTTTGGTTAAAAATTACTGCTCCTGAGAAGTCTGATGAGAACTATAATGAACTGGCAGAAAAGGAATCTAGTGCTGGAACTGCCAAAGATGATTCTGAGGACGAAGTCATGAATCTTACTGTGGCGGGCGCTGTGTGTTTCGTCATAACAGCATCCACTTTTCTGTTGCTACTATACTTCTTTATGTCTACGTGGTTTATCTGGGTGCTGATCATACTTTTCTGCATTGGCGGTATAGAGGGAATGCATAATTGTGTTCTGAGCTTGATTTTGAGAAAATGGACAAGTGGTGGACGGAAGACAATAACTTTGCCTCTTCTGGATGAGGTTTCTGTTCTCTCACTTGTTGTATTGGCTTTCTGTGCGGCATTTGCAATTTTCTGGGTTGTAACACGGCGGGAATCGTATTCATGGATTGGCCAAGATGTTCTTGGTATCTGCTTGATGATAACGATCTTGCAAATTGCTCGATTACCAAATATAAAGGTTGCTACCGTACTACTTTGTTGTGCATTTGTCTACGACATCTTTTGGGTATTCCTATCACCCTTAATATTCAAAGATAGCGTCATGGTTGTGGTTGCTAAAGGTGACAGTAGTGGTGAAGCCCTACCAATGCTCTTGAGAATCCCTCGCTTTTTGGACCCCTGGGGTGGTGTGAATATGATTGGTTTTGGGGATGTTCTATTTCCTGGTTTGCTTATTGTATTTACTTACAGGTTTGACAAAGAAAATAAGAAGAGTGCGTATAACGGATATTTTCTTTGGCTGGTAATTGGCTATGGAATTGGACTCGGTCTTACTTACCTGGCTTTGTATCTGATGAATGGGAACGGTCAGCCTGCACTCCTATATCTTGTTCCATGTACACTAGGTGTTACAGTGATTTTGGGATTGATAAGGTGCGAACTGAAGCAACTCTGGGATTATGGCGCAGAGGTATCCCCATCGAGCGTTGAACCTTCCATAGAAGCCAGTAGATCGGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0005798 GO:0012505 GO:0016020 GO:0016021 GO:0030176 GO:0030660 GO:0031090 GO:0031224 GO:0031227 GO:0031410 GO:0031982 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071458 GO:0071556 GO:0097708 GO:0098552 GO:0098553 GO:0098554 GO:0098562 GO:0098588 GO:0098805 GO:0098827 GO:0098852

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA Peptidase_A22B

Protein Analysis

539

Amino Acids

58.72

Weight (kDa)

5.46

Isoelectric Point (pI)

37.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PA	PF02225	83 - 168	3.8e-13	PA domain
Peptidase_A22B	PF04258	241 - 520	9.2e-94	Signal peptide peptidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005706)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05820 AT1G05820 AT1G05820 AT1G05820 AT2G43070 AT2G43070 AT2G43070 AT2G43070
fragaria_vesca	FvH4_4g11900 FvH4_4g11900 FvH4_4g11900 FvH4_4g11900
malus_domestica	MD04G1030600.v1.1 MD13G1278000.v1.1 MD15G1041300.v1.1
prunus_persica	Prupe.1G180000_v2.0.a1
pyrus_communis	pycom04g02590
rosa_chinensis	RchiOBHm_Chr4g0409391
rosa_laevigata	RLG00000008564
rosa_multiflora	Rmu_sc0001616.1_g000041
rosa_roxburghii	Rroxscaffold_5G00353920
rosa_rugosa	Rorug04G0093500
rosa_samantha	Rh4AG156000 Rh4BG155200 Rh4CG165300 Rh4DG149500
rosa_wichuraiana	Rw0G018710 Rw4G012770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	185
AasI	GACNNNNNNGTC	1 cut(s)	347
Acc16I	TGCGCA	1 cut(s)	366
Acc36I	ACCTGC	1 cut(s)	185
AccI	GTMKAC	2 cut(s)	815, 1146
AccII	CGCG	1 cut(s)	328
AciI	CCGC	4 cut(s)	752, 858, 992, 1024
AcoI	YGGCCR	2 cut(s)	166, 1045
AcuI	CTGAAG	1 cut(s)	1559
AcyI	GRCGYC	1 cut(s)	11
AdeI	CACNNNGTG	1 cut(s)	1502
AfaI	GTAC	3 cut(s)	134, 1125, 1495
AfeI	AGCGCT	1 cut(s)	387
AfiI	CCNNNNNNNGG	2 cut(s)	275, 1280
AflIII	ACRYGT	1 cut(s)	326
AgsI	TTSAA	5 cut(s)	188, 454, 518, 1186, 1586
AjnI	CCWGG	3 cut(s)	1274, 1318, 1434
AluBI	AGCT	5 cut(s)	311, 415, 500, 538, 891
AluI	AGCT	5 cut(s)	311, 415, 500, 538, 891
Alw26I	GTCTC	1 cut(s)	23
Ama87I	CYCGRG	1 cut(s)	66
Aor51HI	AGCGCT	1 cut(s)	387
AoxI	GGCC	2 cut(s)	166, 1045
ApeKI	GCWGC	1 cut(s)	388
ArsI	GACNNNNNNTTYG	4 cut(s)	919, 951, 1179, 1211
Asp700I	GAANNNNTTC	1 cut(s)	183
AspLEI	GCGC	4 cut(s)	367, 388, 758, 1562
AspS9I	GGNCC	1 cut(s)	1270
AsuHPI	GGTGA	5 cut(s)	83, 344, 1164, 1229, 1241
AsuII	TTCGAA	1 cut(s)	296
AvaI	CYCGRG	1 cut(s)	66
AvaII	GGWCC	1 cut(s)	1270
BalI	TGGCCA	1 cut(s)	1047
BbsI	GAAGAC	2 cut(s)	285, 932
BbvI	GCAGC	1 cut(s)	375
BccI	CCATC	5 cut(s)	418, 485, 515, 592, 1582
BceAI	ACGGC	3 cut(s)	153, 224, 1037
BcgI	CGANNNNNNTGC	3 cut(s)	28, 1076, 1110
BciT130I	CCWGG	3 cut(s)	1276, 1320, 1436
BciVI	GTATCC	1 cut(s)	1579
BclI	TGATCA	1 cut(s)	837
BcoDI	GTCTC	1 cut(s)	23
BfaI	CTAG	2 cut(s)	696, 1499
BfoI	RGCGCY	2 cut(s)	389, 759
BfuAI	ACCTGC	1 cut(s)	185
BfuI	GTATCC	1 cut(s)	1579
BglI	GCCNNNNNGGC	1 cut(s)	174
BisI	GCNGC	2 cut(s)	389, 993
BlsI	GCNGC	2 cut(s)	390, 994
Bme1390I	CCNGG	3 cut(s)	1276, 1320, 1436
Bme18I	GGWCC	1 cut(s)	1270
BmeT110I	CYCGRG	1 cut(s)	66
BmgT120I	GGNCC	1 cut(s)	1270
BmiI	GGNNCC	2 cut(s)	480, 1272
BmrFI	CCNGG	3 cut(s)	1276, 1320, 1436
BmsI	GCATC	1 cut(s)	788
BpiI	GAAGAC	2 cut(s)	285, 932
BplI	GAGNNNNNCTC	4 cut(s)	486, 518, 1243, 1275
Bpu14I	TTCGAA	1 cut(s)	296
BpuEI	CTTGAG	2 cut(s)	488, 1270
BsaAI	YACGTR	1 cut(s)	819
BsaBI	GATNNNNATC	1 cut(s)	1079
BsaHI	GRCGYC	1 cut(s)	11
BsaI	GGTCTC	1 cut(s)	23
BsaJI	CCNNGG	3 cut(s)	67, 1274, 1275
Bsc4I	CCNNNNNNNGG	2 cut(s)	275, 1280
Bse1I	ACTGG	3 cut(s)	563, 684, 1602
Bse3DI	GCAATG	1 cut(s)	12
Bse8I	GATNNNNATC	1 cut(s)	1079
BseBI	CCWGG	3 cut(s)	1276, 1320, 1436
BseDI	CCNNGG	3 cut(s)	67, 1274, 1275
BseGI	GGATG	4 cut(s)	97, 779, 955, 1312
BseJI	GATNNNNATC	1 cut(s)	1079
BseLI	CCNNNNNNNGG	2 cut(s)	275, 1280
BseMI	GCAATG	1 cut(s)	12
BseMII	CTCAG	4 cut(s)	591, 642, 714, 878
BseNI	ACTGG	3 cut(s)	563, 684, 1602
BseRI	GAGGAG	1 cut(s)	11
BseXI	GCAGC	1 cut(s)	375
BseYI	CCCAGC	1 cut(s)	107
BsgI	GTGCAG	1 cut(s)	1455
Bsh1236I	CGCG	1 cut(s)	328
BshFI	GGCC	2 cut(s)	168, 1047
BsiHKCI	CYCGRG	1 cut(s)	66
BslI	CCNNNNNNNGG	2 cut(s)	275, 1280
BsmAI	GTCTC	1 cut(s)	23
BsmI	GAATGC	1 cut(s)	876
BsnI	GGCC	2 cut(s)	168, 1047
Bso31I	GGTCTC	1 cut(s)	23
BsoBI	CYCGRG	1 cut(s)	66
Bsp119I	TTCGAA	1 cut(s)	296
Bsp1407I	TGTACA	1 cut(s)	1493
Bsp143I	GATC	3 cut(s)	837, 1080, 1607
BspACI	CCGC	4 cut(s)	752, 858, 992, 1024
BspANI	GGCC	2 cut(s)	168, 1047
BspCNI	CTCAG	4 cut(s)	590, 643, 715, 879
BspFNI	CGCG	1 cut(s)	328
BspHI	TCATGA	1 cut(s)	735
BspLI	GGNNCC	2 cut(s)	480, 1272
BspMI	ACCTGC	1 cut(s)	185
BspQI	GCTCTTC	1 cut(s)	405
BspT104I	TTCGAA	1 cut(s)	296
BspTNI	GGTCTC	1 cut(s)	23
BsrDI	GCAATG	1 cut(s)	12
BsrGI	TGTACA	1 cut(s)	1493
BsrI	ACTGG	3 cut(s)	563, 684, 1602
BssECI	CCNNGG	3 cut(s)	67, 1274, 1275
BssMI	GATC	3 cut(s)	837, 1080, 1607
BssNI	GRCGYC	1 cut(s)	11
Bst2UI	CCWGG	3 cut(s)	1276, 1320, 1436
Bst4CI	ACNGT	5 cut(s)	748, 1123, 1223, 1463, 1510
Bst6I	CTCTTC	3 cut(s)	405, 948, 1361
BstACI	GRCGYC	1 cut(s)	11
BstAPI	GCANNNNNTGC	1 cut(s)	362
BstAUI	TGTACA	1 cut(s)	1493
BstBAI	YACGTR	1 cut(s)	819
BstBI	TTCGAA	1 cut(s)	296
BstC8I	GCNNGC	3 cut(s)	417, 754, 1470
BstDEI	CTNAG	4 cut(s)	577, 651, 723, 887
BstF5I	GGATG	4 cut(s)	97, 779, 955, 1312
BstFNI	CGCG	1 cut(s)	328
BstH2I	RGCGCY	2 cut(s)	389, 759
BstHHI	GCGC	4 cut(s)	367, 388, 758, 1562
BstKTI	GATC	3 cut(s)	840, 1083, 1610
BstMAI	GTCTC	1 cut(s)	23
BstMBI	GATC	3 cut(s)	837, 1080, 1607
BstMWI	GCNNNNNNNGC	5 cut(s)	174, 362, 707, 989, 998
BstNI	CCWGG	3 cut(s)	1276, 1320, 1436
BstSCI	CCNGG	3 cut(s)	1274, 1318, 1434
BstUI	CGCG	1 cut(s)	328
BstV1I	GCAGC	1 cut(s)	375
BstV2I	GAAGAC	2 cut(s)	285, 932
BsuI	GTATCC	1 cut(s)	1579
BsuRI	GGCC	2 cut(s)	168, 1047
BtsCI	GGATG	4 cut(s)	97, 779, 955, 1312
BtsIMutI	CAGTG	2 cut(s)	570, 1515
BveI	ACCTGC	1 cut(s)	185
Cac8I	GCNNGC	3 cut(s)	417, 754, 1470
CciI	TCATGA	1 cut(s)	735
CfoI	GCGC	4 cut(s)	367, 388, 758, 1562
Cfr13I	GGNCC	1 cut(s)	1270
CseI	GACGC	2 cut(s)	548, 1183
Csp6I	GTAC	3 cut(s)	133, 1124, 1494
CviAII	CATG	4 cut(s)	736, 1038, 1198, 1491
CviQI	GTAC	3 cut(s)	133, 1124, 1494
DdeI	CTNAG	4 cut(s)	577, 651, 723, 887
DpnI	GATC	3 cut(s)	839, 1082, 1609
DpnII	GATC	3 cut(s)	837, 1080, 1607
DraIII	CACNNNGTG	1 cut(s)	1502
DrdI	GACNNNNNNGTC	1 cut(s)	347
DseDI	GACNNNNNNGTC	1 cut(s)	347
EaeI	YGGCCR	2 cut(s)	166, 1045
Eam1104I	CTCTTC	3 cut(s)	405, 948, 1361
EarI	CTCTTC	3 cut(s)	405, 948, 1361
Eco31I	GGTCTC	1 cut(s)	23
Eco47I	GGWCC	1 cut(s)	1270
Eco47III	AGCGCT	1 cut(s)	387
Eco57I	CTGAAG	1 cut(s)	1559
Eco88I	CYCGRG	1 cut(s)	66
EcoRII	CCWGG	3 cut(s)	1274, 1318, 1434
EcoT22I	ATGCAT	1 cut(s)	876
FaeI	CATG	4 cut(s)	739, 1041, 1201, 1494
FalI	AAGNNNNNCTT	6 cut(s)	399, 431, 522, 554, 1041, 1073
FatI	CATG	4 cut(s)	735, 1037, 1197, 1490
FauI	CCCGC	2 cut(s)	745, 1017
FbaI	TGATCA	1 cut(s)	837
FblI	GTMKAC	2 cut(s)	815, 1146
Fnu4HI	GCNGC	2 cut(s)	389, 993
FokI	GGATG	4 cut(s)	104, 766, 962, 1319
Fsp4HI	GCNGC	2 cut(s)	389, 993
FspBI	CTAG	2 cut(s)	696, 1499
FspI	TGCGCA	1 cut(s)	366
GlaI	GCGC	4 cut(s)	366, 387, 757, 1561
GluI	GCNGC	2 cut(s)	389, 993
GsaI	CCCAGC	1 cut(s)	111
HaeII	RGCGCY	2 cut(s)	389, 759
HaeIII	GGCC	2 cut(s)	168, 1047
HgaI	GACGC	2 cut(s)	548, 1183
HhaI	GCGC	4 cut(s)	367, 388, 758, 1562
Hin1I	GRCGYC	1 cut(s)	11
Hin1II	CATG	4 cut(s)	739, 1041, 1201, 1494
Hin6I	GCGC	4 cut(s)	365, 386, 756, 1560
HinP1I	GCGC	4 cut(s)	365, 386, 756, 1560
HinfI	GANTC	8 cut(s)	98, 265, 692, 719, 739, 1028, 1254, 1419
HphI	GGTGA	5 cut(s)	83, 344, 1164, 1229, 1241
Hpy166II	GTNNAC	6 cut(s)	324, 571, 816, 920, 1147, 1496
Hpy188I	TCNGA	8 cut(s)	48, 245, 490, 661, 724, 888, 1449, 1616
Hpy188III	TCNNGA	8 cut(s)	262, 372, 434, 505, 650, 736, 947, 1249
Hpy8I	GTNNAC	6 cut(s)	324, 571, 816, 920, 1147, 1496
Hpy99I	CGWCG	1 cut(s)	16
HpyAV	CCTTC	3 cut(s)	126, 231, 1599
HpyCH4III	ACNGT	5 cut(s)	748, 1123, 1223, 1463, 1510
HpyCH4IV	ACGT	1 cut(s)	818
HpyCH4V	TGCA	8 cut(s)	123, 200, 852, 874, 1001, 1087, 1139, 1472
HpyF10VI	GCNNNNNNNGC	5 cut(s)	174, 362, 707, 989, 998
HpyF3I	CTNAG	4 cut(s)	577, 651, 723, 887
HpySE526I	ACGT	1 cut(s)	818
Hsp92I	GRCGYC	1 cut(s)	11
Hsp92II	CATG	4 cut(s)	739, 1041, 1201, 1494
HspAI	GCGC	4 cut(s)	365, 386, 756, 1560
Ksp22I	TGATCA	1 cut(s)	837
Kzo9I	GATC	3 cut(s)	837, 1080, 1607
LguI	GCTCTTC	1 cut(s)	405
LmnI	GCTCC	3 cut(s)	308, 535, 652
Lsp1109I	GCAGC	1 cut(s)	375
LweI	GCATC	1 cut(s)	788
MaeI	CTAG	2 cut(s)	696, 1499
MaeII	ACGT	1 cut(s)	818
MaeIII	GTNAC	4 cut(s)	338, 1015, 1217, 1504
MalI	GATC	3 cut(s)	839, 1082, 1609
MboI	GATC	3 cut(s)	837, 1080, 1607
MboII	GAAGA	8 cut(s)	242, 285, 419, 422, 530, 935, 937, 1378
MlsI	TGGCCA	1 cut(s)	1047
MluCI	AATT	9 cut(s)	154, 462, 618, 639, 877, 1002, 1089, 1401, 1413
MluI	ACGCGT	1 cut(s)	326
MluNI	TGGCCA	1 cut(s)	1047
MlyI	GAGTC	1 cut(s)	1413
MnlI	CCTC	9 cut(s)	32, 62, 368, 718, 859, 946, 951, 1269, 1558
Mox20I	TGGCCA	1 cut(s)	1047
Mph1103I	ATGCAT	1 cut(s)	876
MroXI	GAANNNNTTC	1 cut(s)	183
MscI	TGGCCA	1 cut(s)	1047
MseI	TTAA	6 cut(s)	150, 162, 272, 398, 635, 1178
MslI	CAYNNNNRTG	2 cut(s)	879, 1202
Msp20I	TGGCCA	1 cut(s)	1047
MspR9I	CCNGG	3 cut(s)	1276, 1320, 1436
Mva1269I	GAATGC	1 cut(s)	876
MvaI	CCWGG	3 cut(s)	1276, 1320, 1436
MvnI	CGCG	1 cut(s)	328
MwoI	GCNNNNNNNGC	5 cut(s)	174, 362, 707, 989, 998
NdeII	GATC	3 cut(s)	837, 1080, 1607
NlaIII	CATG	4 cut(s)	739, 1041, 1201, 1494
NlaIV	GGNNCC	2 cut(s)	480, 1272
NmuCI	GTSAC	2 cut(s)	338, 1217
NsbI	TGCGCA	1 cut(s)	366
NsiI	ATGCAT	1 cut(s)	876
NspV	TTCGAA	1 cut(s)	296
PagI	TCATGA	1 cut(s)	735
PaqCI	CACCTGC	1 cut(s)	185
PasI	CCCWGGG	1 cut(s)	1275
PciSI	GCTCTTC	1 cut(s)	405
PctI	GAATGC	1 cut(s)	876
PdmI	GAANNNNTTC	1 cut(s)	183
PfeI	GAWTC	7 cut(s)	98, 265, 692, 719, 739, 1028, 1254
PflFI	GACNNNGTC	2 cut(s)	731, 1422
PkrI	GCNGC	2 cut(s)	390, 994
PleI	GAGTC	1 cut(s)	1413
PpsI	GAGTC	1 cut(s)	1413
Ppu21I	YACGTR	1 cut(s)	819
Psp6I	CCWGG	3 cut(s)	1274, 1318, 1434
PspFI	CCCAGC	1 cut(s)	107
PspGI	CCWGG	3 cut(s)	1274, 1318, 1434
PspN4I	GGNNCC	2 cut(s)	480, 1272
PspPI	GGNCC	1 cut(s)	1270
PsyI	GACNNNGTC	2 cut(s)	731, 1422
RsaI	GTAC	3 cut(s)	134, 1125, 1495
RsaNI	GTAC	3 cut(s)	133, 1124, 1494
RseI	CAYNNNNRTG	2 cut(s)	879, 1202
SapI	GCTCTTC	1 cut(s)	405
SaqAI	TTAA	6 cut(s)	150, 162, 272, 398, 635, 1178
SatI	GCNGC	2 cut(s)	389, 993
Sau3AI	GATC	3 cut(s)	837, 1080, 1607
Sau96I	GGNCC	1 cut(s)	1270
SchI	GAGTC	1 cut(s)	1413
ScrFI	CCNGG	3 cut(s)	1276, 1320, 1436
SfaNI	GCATC	1 cut(s)	788
SfuI	TTCGAA	1 cut(s)	296
SinI	GGWCC	1 cut(s)	1270
SmiMI	CAYNNNNRTG	2 cut(s)	879, 1202
SmlI	CTYRAG	2 cut(s)	503, 1249
SmoI	CTYRAG	2 cut(s)	503, 1249
Sse9I	AATT	9 cut(s)	154, 462, 618, 639, 877, 1002, 1089, 1401, 1413
SsiI	CCGC	4 cut(s)	752, 858, 992, 1024
SspI	AATATT	1 cut(s)	1182
SspMI	CTAG	2 cut(s)	696, 1499
StyD4I	CCNGG	3 cut(s)	1274, 1318, 1434
TaaI	ACNGT	5 cut(s)	748, 1123, 1223, 1463, 1510
TaiI	ACGT	1 cut(s)	821
TaqI	TCGA	5 cut(s)	96, 263, 296, 1096, 1577
TaqII	GACCGA	2 cut(s)	1412, 1600
TasI	AATT	9 cut(s)	154, 462, 618, 639, 877, 1002, 1089, 1401, 1413
TatI	WGTACW	1 cut(s)	1493
TauI	GCSGC	1 cut(s)	995
TfiI	GAWTC	7 cut(s)	98, 265, 692, 719, 739, 1028, 1254
Tru1I	TTAA	6 cut(s)	150, 162, 272, 398, 635, 1178
Tru9I	TTAA	6 cut(s)	150, 162, 272, 398, 635, 1178
TscAI	CASTG	2 cut(s)	570, 1515
TseFI	GTSAC	2 cut(s)	338, 1217
TseI	GCWGC	1 cut(s)	388
Tsp45I	GTSAC	2 cut(s)	338, 1217
TspDTI	ATGAA	6 cut(s)	420, 615, 690, 752, 1026, 1466
TspGWI	ACGGA	4 cut(s)	14, 337, 937, 1395
TspRI	CASTG	2 cut(s)	570, 1515
Tth111I	GACNNNGTC	2 cut(s)	731, 1422
VpaK11BI	GGWCC	1 cut(s)	1270
XcmI	CCANNNNNNNNNTGG	2 cut(s)	562, 1055
XmiI	GTMKAC	2 cut(s)	815, 1146
XmnI	GAANNNNTTC	1 cut(s)	183
XspI	CTAG	2 cut(s)	696, 1499
Zsp2I	ATGCAT	1 cut(s)	876

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.