Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0005706

Physical Location & Seq

Genomic Coordinates Reverse (-)

3434780 .. 3442634

Gene Structure 7855 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1030600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1623 bp

ATGGCAATGGCAATGGCGTCGGGTCTCCTCCGCCTAGTGTTTCTGTTATCTCTGATTGCTCTTTCTTTGGCCCGTGGTGAAAAGGACATGGTTTTGGATGTGGATTCCACCCCCGACACTCCTTCCTGCAACAACCCTTACCTAATGGCAAAGGTTAAGAATTGGGTTAATGGCCATGAAGGAGAAACTATTGAAGGGGCAGGTGCAAAGTTTGGGGCTTTATTGCCTTCCAAAGAAGAAAAAGCCGTCAAATTGCCCGTTGTTATCTCGAATCCTTTAAATGGTTGTTCAGCGTCATCTTCGAAGTTATCTGGAGCTATTGCCTTGTCCACGCGTGGTGATTGTGACTTCTCCGTAAAGGCAAAAGTTGCACAGTCAGGAGGTGCTAAGGCGCTAGTGGTGATAAATAATGATGAAGAGCTTGCCAAGATGGCTTGTCCTGATAATAGTACTTCTTTGAATATTTCAATTTTTGTCGTAATGGTTCCAAAGTCAGATGGAGAAGCACTCAAGAAATCTATTGAAGATGGAAAGAAAGTGGAGCTTCTATTATATTCTCCCAAGCGTCCAGTGGTGGACTACTCAGTTGTGTTCCTGTGGCTGATGGCTGTTGGAACAATAATAGTTGCCTCATTTTGGTCAAAGATTACTGCTCCTGATAAGTCTGATGAGAATTATAATGAACTAGCAGAAAAGGAATCTAATACTGGAACAGCCAAAGATGATTCTGAGGACGAAGTCATGAATCTTAGTGTGGCAGGCGCTGTGTGTTTCGTCATAACAGCATCCACTTTTCTGTTGCTACTATATTTCTTTATGTCTACTTGGTTTATCTGGGTGCTGATTGTACTTTTCTGCATTGGTGGTATTGAGGGAATGCATAATTGTGTTCTGAGCCTGATTTTGAGAAAATGGAGAAGTGGTGGGCAGAAGACGATAACTTTGCCTCTTCTGGATGAGGTTTCTATTCTCTCGCTTGTTGTATTGGCTTTCTGTGCGGCATTTGCAGTTTTCTGGGTTGTTACCCGGCGGGCGTCATATTCATGGATTGGCCAAGATGTTCTTGGTATCTGCTTGATGATAACGGTCTTGCAAATTGCTCGATTGCCTAACATAAAGGTTGCTACCGTACTACTTTGTTGTGCATTTGTCTATGACATCTTTTGGGTATTCCTGTCACCCTTAATATTCAAAGATAGCGTCATGGTTACGGTTGCTAAAGGTGACGGCAGTGGTGAAGCCCTACCAATGCTTTTGAGAATCCCTCGCTTTTTTGACCCCTGGGGTGGTGTGAATATGATTGGCTTCGGGGACGTTCTGTTTCCTGGTTTGCTTATTGTATTTACTTACAGGTTTGACAAAGAAAATAAGAAGAGTGCGTTTAACGGATATTTTCCCTGGTTATTAATTGGCTATGGAATTGGACTCGGTCTTACTTACCTGGGTTTGTATCTCATGAACGGCAACGGCCAGCCTGCACTCCTCTATCTCGTTCCATGTACACTAGGTGTTACAGTGATTTTGGGACTGATAAGGCGCGAGCTGAAACAACTCTGGGATTACGGCGCAGAGGTATCTCCATCGAGCGTTGAACCGTCCGTAGACGCCAGTAGATCGGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0005798 GO:0012505 GO:0016020 GO:0016021 GO:0030176 GO:0030660 GO:0031090 GO:0031224 GO:0031227 GO:0031410 GO:0031982 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071458 GO:0071556 GO:0097708 GO:0098552 GO:0098553 GO:0098554 GO:0098562 GO:0098588 GO:0098805 GO:0098827 GO:0098852

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PA Peptidase_A22B

Protein Analysis

541

Amino Acids

58.72

Weight (kDa)

5.55

Isoelectric Point (pI)

35.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PA	PF02225	83 - 170	4.5e-15	PA domain
Peptidase_A22B	PF04258	244 - 522	1.2e-94	Signal peptide peptidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005706)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05820 AT1G05820 AT1G05820 AT1G05820 AT2G43070 AT2G43070 AT2G43070 AT2G43070
fragaria_vesca	FvH4_4g11900 FvH4_4g11900 FvH4_4g11900 FvH4_4g11900
malus_domestica	MD04G1030600.v1.1 MD13G1278000.v1.1 MD15G1041300.v1.1
prunus_persica	Prupe.1G180000_v2.0.a1
pyrus_communis	pycom04g02590
rosa_chinensis	RchiOBHm_Chr4g0409391
rosa_laevigata	RLG00000008564
rosa_multiflora	Rmu_sc0001616.1_g000041
rosa_roxburghii	Rroxscaffold_5G00353920
rosa_rugosa	Rorug04G0093500
rosa_samantha	Rh4AG156000 Rh4BG155200 Rh4CG165300 Rh4DG149500
rosa_wichuraiana	Rw0G018710 Rw4G012770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	678
AarI	CACCTGC	1 cut(s)	191
Acc36I	ACCTGC	1 cut(s)	191
AccI	GTMKAC	2 cut(s)	821, 1602
AccII	CGCG	2 cut(s)	334, 1539
AciI	CCGC	3 cut(s)	31, 998, 1030
AcoI	YGGCCR	3 cut(s)	172, 1051, 1468
AcyI	GRCGYC	3 cut(s)	17, 1034, 1605
AdeI	CACNNNGTG	1 cut(s)	1508
AfaI	GTAC	4 cut(s)	451, 849, 1131, 1501
AfiI	CCNNNNNNNGG	3 cut(s)	281, 636, 1286
AflIII	ACRYGT	1 cut(s)	332
AgsI	TTSAA	6 cut(s)	194, 460, 468, 524, 1192, 1592
AjnI	CCWGG	4 cut(s)	1280, 1324, 1397, 1440
AluBI	AGCT	4 cut(s)	317, 421, 544, 1543
AluI	AGCT	4 cut(s)	317, 421, 544, 1543
Alw26I	GTCTC	1 cut(s)	29
AlwNI	CAGNNNCTG	1 cut(s)	764
AoxI	GGCC	4 cut(s)	69, 172, 1051, 1468
ArsI	GACNNNNNNTTYG	4 cut(s)	925, 957, 1185, 1217
AseI	ATTAAT	1 cut(s)	1406
AspLEI	GCGC	4 cut(s)	394, 764, 1539, 1568
AspS9I	GGNCC	1 cut(s)	70
AsuC2I	CCSGG	1 cut(s)	1027
AsuHPI	GGTGA	6 cut(s)	89, 350, 412, 1170, 1235, 1247
AsuII	TTCGAA	1 cut(s)	302
BalI	TGGCCA	2 cut(s)	174, 1053
BbsI	GAAGAC	1 cut(s)	938
BccI	CCATC	5 cut(s)	424, 491, 521, 598, 1588
BceAI	ACGGC	5 cut(s)	230, 1243, 1477, 1483, 1579
BcgI	CGANNNNNNTGC	5 cut(s)	34, 925, 959, 1082, 1116
BciT130I	CCWGG	4 cut(s)	1282, 1326, 1399, 1442
BcnI	CCSGG	1 cut(s)	1027
BcoDI	GTCTC	1 cut(s)	29
BfaI	CTAG	4 cut(s)	35, 395, 686, 1505
BfoI	RGCGCY	2 cut(s)	395, 765
BfuAI	ACCTGC	1 cut(s)	191
BglI	GCCNNNNNGGC	1 cut(s)	431
BisI	GCNGC	1 cut(s)	999
BlsI	GCNGC	1 cut(s)	1000
BmcAI	AGTACT	1 cut(s)	451
Bme1390I	CCNGG	5 cut(s)	1027, 1282, 1326, 1399, 1442
BmgT120I	GGNCC	1 cut(s)	70
BmiI	GGNNCC	1 cut(s)	486
BmrFI	CCNGG	5 cut(s)	1027, 1282, 1326, 1399, 1442
BmsI	GCATC	1 cut(s)	794
BpiI	GAAGAC	1 cut(s)	938
BplI	GAGNNNNNCTC	4 cut(s)	492, 524, 1249, 1281
BpmI	CTGGAG	1 cut(s)	333
Bpu10I	CCTNAGC	1 cut(s)	387
Bpu14I	TTCGAA	1 cut(s)	302
BpuEI	CTTGAG	1 cut(s)	494
BpuMI	CCSGG	1 cut(s)	1027
BsaHI	GRCGYC	3 cut(s)	17, 1034, 1605
BsaI	GGTCTC	1 cut(s)	29
BsaJI	CCNNGG	5 cut(s)	73, 1280, 1281, 1397, 1441
BsaXI	ACNNNNNCTCC	2 cut(s)	907, 937
Bsc4I	CCNNNNNNNGG	3 cut(s)	281, 636, 1286
Bse1I	ACTGG	3 cut(s)	569, 712, 1608
Bse3DI	GCAATG	2 cut(s)	12, 18
BseBI	CCWGG	4 cut(s)	1282, 1326, 1399, 1442
BseDI	CCNNGG	5 cut(s)	73, 1280, 1281, 1397, 1441
BseGI	GGATG	3 cut(s)	103, 785, 961
BseLI	CCNNNNNNNGG	3 cut(s)	281, 636, 1286
BseMI	GCAATG	2 cut(s)	12, 18
BseMII	CTCAG	3 cut(s)	597, 720, 884
BseNI	ACTGG	3 cut(s)	569, 712, 1608
BseRI	GAGGAG	2 cut(s)	17, 1472
BsgI	GTGCAG	1 cut(s)	1461
Bsh1236I	CGCG	2 cut(s)	334, 1539
BshFI	GGCC	4 cut(s)	71, 174, 1053, 1470
BsiSI	CCGG	1 cut(s)	1027
BslFI	GGGAC	2 cut(s)	1325, 1539
BslI	CCNNNNNNNGG	3 cut(s)	281, 636, 1286
BsmAI	GTCTC	1 cut(s)	29
BsmFI	GGGAC	2 cut(s)	1325, 1539
BsmI	GAATGC	1 cut(s)	882
BsnI	GGCC	4 cut(s)	71, 174, 1053, 1470
Bso31I	GGTCTC	1 cut(s)	29
Bsp119I	TTCGAA	1 cut(s)	302
Bsp1407I	TGTACA	1 cut(s)	1499
Bsp143I	GATC	1 cut(s)	1613
BspACI	CCGC	3 cut(s)	31, 998, 1030
BspANI	GGCC	4 cut(s)	71, 174, 1053, 1470
BspCNI	CTCAG	3 cut(s)	596, 721, 885
BspFNI	CGCG	2 cut(s)	334, 1539
BspHI	TCATGA	2 cut(s)	741, 1455
BspLI	GGNNCC	1 cut(s)	486
BspMI	ACCTGC	1 cut(s)	191
BspQI	GCTCTTC	1 cut(s)	411
BspT104I	TTCGAA	1 cut(s)	302
BspTNI	GGTCTC	1 cut(s)	29
BsrDI	GCAATG	2 cut(s)	12, 18
BsrGI	TGTACA	1 cut(s)	1499
BsrI	ACTGG	3 cut(s)	569, 712, 1608
BssECI	CCNNGG	5 cut(s)	73, 1280, 1281, 1397, 1441
BssMI	GATC	1 cut(s)	1613
BssNI	GRCGYC	3 cut(s)	17, 1034, 1605
Bst2UI	CCWGG	4 cut(s)	1282, 1326, 1399, 1442
Bst4CI	ACNGT	6 cut(s)	375, 1087, 1129, 1213, 1516, 1596
Bst6I	CTCTTC	3 cut(s)	411, 954, 1367
BstACI	GRCGYC	3 cut(s)	17, 1034, 1605
BstAPI	GCANNNNNTGC	1 cut(s)	368
BstAUI	TGTACA	1 cut(s)	1499
BstBI	TTCGAA	1 cut(s)	302
BstC8I	GCNNGC	6 cut(s)	423, 760, 1032, 1472, 1476, 1541
BstDEI	CTNAG	5 cut(s)	387, 583, 729, 749, 893
BstDSI	CCRYGG	1 cut(s)	73
BstF5I	GGATG	3 cut(s)	103, 785, 961
BstFNI	CGCG	2 cut(s)	334, 1539
BstH2I	RGCGCY	2 cut(s)	395, 765
BstHHI	GCGC	4 cut(s)	394, 764, 1539, 1568
BstKTI	GATC	1 cut(s)	1616
BstMAI	GTCTC	1 cut(s)	29
BstMBI	GATC	1 cut(s)	1613
BstMWI	GCNNNNNNNGC	4 cut(s)	368, 431, 995, 1004
BstNI	CCWGG	4 cut(s)	1282, 1326, 1399, 1442
BstSCI	CCNGG	5 cut(s)	1025, 1280, 1324, 1397, 1440
BstUI	CGCG	2 cut(s)	334, 1539
BstV2I	GAAGAC	1 cut(s)	938
BsuRI	GGCC	4 cut(s)	71, 174, 1053, 1470
BtgI	CCRYGG	1 cut(s)	73
BtsCI	GGATG	3 cut(s)	103, 785, 961
BtsI	GCAGTG	1 cut(s)	1237
BtsIMutI	CAGTG	3 cut(s)	576, 1237, 1521
BveI	ACCTGC	1 cut(s)	191
Cac8I	GCNNGC	6 cut(s)	423, 760, 1032, 1472, 1476, 1541
CaiI	CAGNNNCTG	1 cut(s)	764
CciI	TCATGA	2 cut(s)	741, 1455
CfoI	GCGC	4 cut(s)	394, 764, 1539, 1568
Cfr13I	GGNCC	1 cut(s)	70
CseI	GACGC	6 cut(s)	6, 282, 554, 1023, 1189, 1613
Csp6I	GTAC	4 cut(s)	450, 848, 1130, 1500
CviAII	CATG	7 cut(s)	88, 176, 742, 1044, 1204, 1456, 1497
CviQI	GTAC	4 cut(s)	450, 848, 1130, 1500
DdeI	CTNAG	5 cut(s)	387, 583, 729, 749, 893
DpnI	GATC	1 cut(s)	1615
DpnII	GATC	1 cut(s)	1613
DraI	TTTAAA	1 cut(s)	279
DraIII	CACNNNGTG	1 cut(s)	1508
EaeI	YGGCCR	3 cut(s)	172, 1051, 1468
Eam1104I	CTCTTC	3 cut(s)	411, 954, 1367
EarI	CTCTTC	3 cut(s)	411, 954, 1367
EciI	GGCGGA	1 cut(s)	20
Eco31I	GGTCTC	1 cut(s)	29
EcoRII	CCWGG	4 cut(s)	1280, 1324, 1397, 1440
EcoT22I	ATGCAT	1 cut(s)	882
FaeI	CATG	7 cut(s)	91, 179, 745, 1047, 1207, 1459, 1500
FalI	AAGNNNNNCTT	4 cut(s)	528, 560, 1047, 1079
FaqI	GGGAC	2 cut(s)	1325, 1539
FatI	CATG	7 cut(s)	87, 175, 741, 1043, 1203, 1455, 1496
FauI	CCCGC	1 cut(s)	1023
FblI	GTMKAC	2 cut(s)	821, 1602
Fnu4HI	GCNGC	1 cut(s)	999
FokI	GGATG	3 cut(s)	110, 772, 968
Fsp4HI	GCNGC	1 cut(s)	999
FspBI	CTAG	4 cut(s)	35, 395, 686, 1505
GlaI	GCGC	4 cut(s)	393, 763, 1538, 1567
GluI	GCNGC	1 cut(s)	999
GsuI	CTGGAG	1 cut(s)	333
HaeII	RGCGCY	2 cut(s)	395, 765
HaeIII	GGCC	4 cut(s)	71, 174, 1053, 1470
HapII	CCGG	1 cut(s)	1027
HgaI	GACGC	6 cut(s)	6, 282, 554, 1023, 1189, 1613
HhaI	GCGC	4 cut(s)	394, 764, 1539, 1568
Hin1I	GRCGYC	3 cut(s)	17, 1034, 1605
Hin1II	CATG	7 cut(s)	91, 179, 745, 1047, 1207, 1459, 1500
Hin6I	GCGC	4 cut(s)	392, 762, 1537, 1566
HinP1I	GCGC	4 cut(s)	392, 762, 1537, 1566
HinfI	GANTC	7 cut(s)	104, 271, 698, 725, 745, 1260, 1425
HpaII	CCGG	1 cut(s)	1027
HphI	GGTGA	6 cut(s)	89, 350, 412, 1170, 1235, 1247
Hpy166II	GTNNAC	5 cut(s)	330, 577, 822, 1502, 1603
Hpy188I	TCNGA	6 cut(s)	54, 496, 667, 730, 894, 1622
Hpy188III	TCNNGA	9 cut(s)	268, 312, 378, 440, 511, 656, 742, 953, 1456
Hpy8I	GTNNAC	5 cut(s)	330, 577, 822, 1502, 1603
Hpy99I	CGWCG	1 cut(s)	22
HpyAV	CCTTC	4 cut(s)	132, 173, 188, 237
HpyCH4III	ACNGT	6 cut(s)	375, 1087, 1129, 1213, 1516, 1596
HpyCH4IV	ACGT	1 cut(s)	1314
HpyCH4V	TGCA	9 cut(s)	129, 206, 371, 858, 880, 1007, 1093, 1145, 1478
HpyF10VI	GCNNNNNNNGC	4 cut(s)	368, 431, 995, 1004
HpyF3I	CTNAG	5 cut(s)	387, 583, 729, 749, 893
HpySE526I	ACGT	1 cut(s)	1314
Hsp92I	GRCGYC	3 cut(s)	17, 1034, 1605
Hsp92II	CATG	7 cut(s)	91, 179, 745, 1047, 1207, 1459, 1500
HspAI	GCGC	4 cut(s)	392, 762, 1537, 1566
Kzo9I	GATC	1 cut(s)	1613
LguI	GCTCTTC	1 cut(s)	411
LmnI	GCTCC	3 cut(s)	314, 541, 658
LweI	GCATC	1 cut(s)	794
MaeI	CTAG	4 cut(s)	35, 395, 686, 1505
MaeII	ACGT	1 cut(s)	1314
MaeIII	GTNAC	6 cut(s)	344, 1021, 1176, 1207, 1223, 1510
MalI	GATC	1 cut(s)	1615
MboI	GATC	1 cut(s)	1613
MboII	GAAGA	7 cut(s)	248, 291, 428, 536, 941, 943, 1384
MlsI	TGGCCA	2 cut(s)	174, 1053
MluCI	AATT	8 cut(s)	160, 251, 468, 673, 883, 1095, 1407, 1419
MluI	ACGCGT	1 cut(s)	332
MluNI	TGGCCA	2 cut(s)	174, 1053
MlyI	GAGTC	1 cut(s)	1419
MmeI	TCCRAC	1 cut(s)	592
Mox20I	TGGCCA	2 cut(s)	174, 1053
Mph1103I	ATGCAT	1 cut(s)	882
MscI	TGGCCA	2 cut(s)	174, 1053
MseI	TTAA	6 cut(s)	156, 168, 278, 1184, 1383, 1406
MslI	CAYNNNNRTG	2 cut(s)	885, 1042
Msp20I	TGGCCA	2 cut(s)	174, 1053
MspI	CCGG	1 cut(s)	1027
MspR9I	CCNGG	5 cut(s)	1027, 1282, 1326, 1399, 1442
Mva1269I	GAATGC	1 cut(s)	882
MvaI	CCWGG	4 cut(s)	1282, 1326, 1399, 1442
MvnI	CGCG	2 cut(s)	334, 1539
MwoI	GCNNNNNNNGC	4 cut(s)	368, 431, 995, 1004
NciI	CCSGG	1 cut(s)	1027
NdeII	GATC	1 cut(s)	1613
NlaIII	CATG	7 cut(s)	91, 179, 745, 1047, 1207, 1459, 1500
NlaIV	GGNNCC	1 cut(s)	486
NmuCI	GTSAC	3 cut(s)	344, 1176, 1223
NsiI	ATGCAT	1 cut(s)	882
NspV	TTCGAA	1 cut(s)	302
PagI	TCATGA	2 cut(s)	741, 1455
PaqCI	CACCTGC	1 cut(s)	191
PasI	CCCWGGG	1 cut(s)	1281
PciSI	GCTCTTC	1 cut(s)	411
PctI	GAATGC	1 cut(s)	882
PfeI	GAWTC	6 cut(s)	104, 271, 698, 725, 745, 1260
PflFI	GACNNNGTC	2 cut(s)	737, 1428
PkrI	GCNGC	1 cut(s)	1000
PleI	GAGTC	1 cut(s)	1419
PpsI	GAGTC	1 cut(s)	1419
PshBI	ATTAAT	1 cut(s)	1406
PsiI	TTATAA	1 cut(s)	678
Psp6I	CCWGG	4 cut(s)	1280, 1324, 1397, 1440
PspGI	CCWGG	4 cut(s)	1280, 1324, 1397, 1440
PspN4I	GGNNCC	1 cut(s)	486
PspPI	GGNCC	1 cut(s)	70
PstNI	CAGNNNCTG	1 cut(s)	764
PsyI	GACNNNGTC	2 cut(s)	737, 1428
RsaI	GTAC	4 cut(s)	451, 849, 1131, 1501
RsaNI	GTAC	4 cut(s)	450, 848, 1130, 1500
RseI	CAYNNNNRTG	2 cut(s)	885, 1042
SapI	GCTCTTC	1 cut(s)	411
SaqAI	TTAA	6 cut(s)	156, 168, 278, 1184, 1383, 1406
SatI	GCNGC	1 cut(s)	999
Sau3AI	GATC	1 cut(s)	1613
Sau96I	GGNCC	1 cut(s)	70
ScaI	AGTACT	1 cut(s)	451
SchI	GAGTC	1 cut(s)	1419
ScrFI	CCNGG	5 cut(s)	1027, 1282, 1326, 1399, 1442
SfaNI	GCATC	1 cut(s)	794
SfuI	TTCGAA	1 cut(s)	302
SmiMI	CAYNNNNRTG	2 cut(s)	885, 1042
SmlI	CTYRAG	1 cut(s)	509
SmoI	CTYRAG	1 cut(s)	509
Sse9I	AATT	8 cut(s)	160, 251, 468, 673, 883, 1095, 1407, 1419
SsiI	CCGC	3 cut(s)	31, 998, 1030
SspI	AATATT	2 cut(s)	463, 1188
SspMI	CTAG	4 cut(s)	35, 395, 686, 1505
StyD4I	CCNGG	5 cut(s)	1025, 1280, 1324, 1397, 1440
TaaI	ACNGT	6 cut(s)	375, 1087, 1129, 1213, 1516, 1596
TaiI	ACGT	1 cut(s)	1317
TaqI	TCGA	4 cut(s)	269, 302, 1102, 1583
TaqII	GACCGA	2 cut(s)	1418, 1606
TasI	AATT	8 cut(s)	160, 251, 468, 673, 883, 1095, 1407, 1419
TatI	WGTACW	3 cut(s)	449, 847, 1499
TauI	GCSGC	1 cut(s)	1001
TfiI	GAWTC	6 cut(s)	104, 271, 698, 725, 745, 1260
Tru1I	TTAA	6 cut(s)	156, 168, 278, 1184, 1383, 1406
Tru9I	TTAA	6 cut(s)	156, 168, 278, 1184, 1383, 1406
TscAI	CASTG	3 cut(s)	576, 1237, 1521
TseFI	GTSAC	3 cut(s)	344, 1176, 1223
Tsp45I	GTSAC	3 cut(s)	344, 1176, 1223
TspDTI	ATGAA	6 cut(s)	192, 429, 696, 758, 1032, 1472
TspGWI	ACGGA	3 cut(s)	343, 1401, 1588
TspRI	CASTG	3 cut(s)	576, 1237, 1521
Tth111I	GACNNNGTC	2 cut(s)	737, 1428
VspI	ATTAAT	1 cut(s)	1406
XcmI	CCANNNNNNNNNTGG	2 cut(s)	568, 1061
XmiI	GTMKAC	2 cut(s)	821, 1602
XspI	CTAG	4 cut(s)	35, 395, 686, 1505
ZrmI	AGTACT	1 cut(s)	451
Zsp2I	ATGCAT	1 cut(s)	882

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.