AT1G06490

1,3-beta-glucan synthase component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009173
Physical Location & Seq
Forward (+)
1978378 .. 1989409
11032 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G06490.2

Sequence Viewer

Length: 5877 bp
ATGGCGAGTACTAGTAGTGGTGGAAGAGGCGAAGATGGGCGGCCGCCGCAAATGCAGCCAGTTCGGTCTATGTCTCGGAAGATGACACGAGCTGGTACGATGATGATTGAGCATCCTAATGAAGATGAAAGACCAATTGACAGCGAGCTTGTTCCTTCTTCTCTCGCTTCCATTGCTCCTATTCTTCGAGTTGCTAATGATATTGACCAAGATAACGCAAGGGTTGCTTACCTTTGTCGCTTCCATGCATTTGAGAAGGCTCATCGAATGGATCCAACATCAAGCGGACGAGGCGTTCGTCAATTTAAGACTTATCTATTGCACAAGCTAGAGGAGGAGGAAGAGATTACAGAACATATGTTGGCGAAGAGTGACCCAAGAGAAATTCAGCTCTACTATCAGACGTTCTATGAGAATAATATACAAGATGGAGAAGGGAAAAAGACACCGGAGGAGATGGCCAAGCTTTACCAGATAGCGACAGTCTTGTATGATGTTTTAAAGACAGTGGTTCCTCAAGCAAGAATTGACGACAAGACCCTTAGGTATGCCAAAGAAGTTGAAAGAAAGAAAGAACAGTATGAACACTACAACATTCTTCCGCTGTACGCTTTAGGGGCTAAAACTGCAGTAATGGAACTCCCTGAGATTAAGGCGGCAATTCTTGCTGTCTGCAATGTGGACAATCTTCCTCGACCAAGATTCCATTCAGCCTCAGCTAACCTGGATGAAGTGGATAGAGAAAGAGGCAGATCTTTTAATGATATTCTCGAGTGGCTTGCTTTGGTTTTCGGTTTTCAGCGAGGAAATGTAGCTAATCAGAGGGAGCATCTTATACTGCTACTTGCCAACATCGATGTGAGGAAGAGGGATCTCGAGAATTATGTCGAGATAAAGCCTAGCACCGTGCGGAAATTGATGGAAAAATACTTCAAGAATTACAATTCATGGTGCAAGTATTTGCGGTGTGACTCATATTTGAGGTTTCCTGCAGGTTGTGATAAGCAACAACTGAGCCTTCTGTATATCGGCCTTTATCTTCTCATATGGGGAGAAGCATCAAATGTCCGCTTCATGCCTGAATGCCTTTGCTACATTTTCCACAATATGGCCAATGAAGTCCATGGAATTCTGTTTGGCAATGTGTACCCTGTAACTGGGGACACATATGAGGCAGGTGCACCGGACGAAGAAGCATTCTTAAGGAATGTTATAACACCAATCTACCAGGTCTTACGCAAGGAAGTCAGGAGAAACAAAAATGGAAAGGCAAGCCATTCAAAATGGAGAAACTATGATGATCTGAATGAGTACTTTTGGGATAAGAGGTGTTTTAGGCTGAAATGGCCAATGAACTTTAAGGCAGATTTTTTCATCCACACTGATGAGATCTCGCAAGTTCCGAATCAGAGACATGACCAAGTGTCTCATGGAAAAAGGAAACCTAAAACTAATTTTGTTGAAGCTCGCACGTTTTGGAACCTCTATCGAAGTTTCGACCGAATGTGGATGTTCCTTGTATTGTCTCTACAGACTATGATAATAGTTGCATGGCACCCGTCAGGATCTATTCTTGCCATTTTTACTGAGGATGTCTTTAGAAACGTGTTGACCATTTTCATAACTTCAGCTTTTCTCAATCTTCTACAAGCGACACTGGATTTAGTTCTTAGTTTTGGTGCTTGGAAGAGCCTAAAGTTTTCCCAAATTATGCGGTACATCACAAAATTTTTAATGGCAGCCATGTGGGCCATCATGTTGCCAATTACTTACTCGAAGTCAGTCCAGAATCCTACTGGACTAATAAAGTTCTTCAGCAGCTGGGTTGGGAGTTGGCTTCACCGGTCGTTATACGACTATGCTATTGCCTTGTATGTCTTACCAAATATTTTGGCTGCTGTGTTTTTCTTACTTCCACCTCTGCGGAGAATCATGGAGCGATCAAACATGCGGATTGTCACACTTATCATGTGGTGGGCTCAGCCAAAATTGTATATTGGTAGAGGAATGCATGAAGAAATGTTTGCACTTTTCAAGTATACATTCTTTTGGGTCATGCTGTTACTTAGCAAGCTCGCTTTCAGCTACTATGTGGAGATATTACCACTTGTCAACCCAACTAAGTTAATATGGGATATGCATGTCGTAAATTACGAGTGGCATGAGTTCTTTCCAAATGCCACTCATAACATTGGGGTAATCATCGCCATATGGGGTCCAATTGTCTTGGTTTACTTTATGGATACGCAAATATGGTATGCCATATTCTCTACTCTTTTTGGAGGGATATATGGAGCCTTCAGCCATCTCGGGGAGATACGCACACTTGGAATGTTGCGGTCCAGATTTAAAGTTGTTCCATCTGCTTTCTGTTCTAAACTTACACCGTTACCTCTAGGTCATGCAAAGAGAAAGCACTTGGACGAAACGGTGGACGAAAAAGACATTGCGAGGTTCTCACAGATGTGGAATAAGTTCATACATACTATGCGGGATGAAGATCTGATCAGCGATAGGGAAAGAGATTTGCTACTTGTACCTTCGTCTTCTGGAGATGTTACTGTTGTGCAATGGCCTCCTTTCTTGCTTGCTAGCAAGATTCCAATAGCATTAGACATGGCTAAAGATTTCAAGGGAAAAGAGGACGTTGACTTATTCAAGAAAATCAAAAGCGAATACTATATGCATTACGCAGTGGTTGAGGCTTATGAAACCGTGAGAGACATAATATATGGCCTTCTTCAAGATGAATCTGATAAGAGGATTGTAAGGGAGATTTGCTACGAAGTAGATATTAGCATCCAACAACACAGATTTTTAAGTGAATTCCGGATGACTGGTATGCCTTTGCTCAGTGACAAGCTAGAGAAATTCTTAAAAATCCTGCTAAGTGATTATGAGGAAGATGATTACAAGTCTCAGATTATCAATGTTCTCCAGGATATTATTGAAATCATCACCCAGGATGTCATGGTTAACGGTCACGAAATCCTTGAAAGGGCTCATTTACAGAGTGGTGATATTGAAAGCGACAAGAAAGAACAAAGGTTTGAAAAGATAGATCTTAGTTTGACTCAGAACATATCCTGGAGGGAAAAGGTTGTAAGACTTTTGTTACTTTTGACTGTTAAAGAATCCGCAATAAATATCCCTCAAAGTTTGGAAGCTCGTCGTCGTATGACATTCTTTGCAAATTCCTTATTCATGAATATGCCAGATGCTCCCCGAGTTCGTGACATGTTATCCTTTAGTGTCTTAACTCCTTATTACAAAGAGGATGTTCTCTATTCGGAGGAAGAACTGAACAAGGAAAACGAAGATGGAATAACTATTTTGTTTTACCTACAGAGGATATATCCTGAGGAATGGTCAAACTATTGCGAACGTGTAAATGATTTGAAACGCAACTTATCTGAGAAAGACAAGGCAGAGCAACTCCGCCAATGGGTATCTTATAGAGGCCAGACTCTTTCAAGGACAGTTAGGGGAATGATGTACTACAGAGTGGCTCTCGAACTTCAATGTTTCCAGGAATACACTGAAGAAAACGCTACCAATGGTGGCTATCTTCCCTCAGAATCAAATGAAGATGATCGAAAGGCTTTTAGTGACCGTGCACGAGCTCTTGCAGATTTAAAATTTACATATGTCGTGTCCTGTCAGGTCTATGGGAACCAGAAAAAGTCAAGTGAAAGCCGAGACCGGAGTTGTTACAATAACATTTTACAGCTCATGCTAAAGTATCCGTCATTGCGTGTTGCCTATATAGATGAAAGGGAGGAGACAGTTAATGGAAAATCACAGAAAGTATTCTACTCTGTGCTACTCAAAGGATGTGACAAATTGGATGAGGAAATATATCGTATCAAACTTCCCGGCCCTCCCACTGAAATTGGTGAAGGAAAACCCGAGAATCAAAACCATGCCATCATTTTCACTCGCGGTGAAGCACTCCAGACCATAGACATGAACCAGGACAACTACTTTGAAGAGTGTTTCAAAATGAGAAACGTGCTACAAGAATTTGATGAAGGTCGACGTGGCAAGAGAAATCCTACAATTTTGGGTCTTCGTGAACACATATTTACTGGAAGTGTTTCTTCACTTGCTTGGTTCATGTCGAATCAAGAAACAAGTTTTGTAACCATTGGCCAACGTGTTCTGGCAAATCCTCTGAGGGTGCGTTTCCATTATGGCCATCCTGATATATTTGATAGAATCTTCCACATCACAAGAGGAGGCATTAGCAAGGCTTCAAAGATAATAAACTTAAGCGAAGATATCTTTGCAGGGTACAATTCAACTCTTCGTGGGGGTTACGTCACACATCACGAGTATATTCAAGCAGGGAAAGGTCGTGATGTAGGGATGAATCAGATTTCGTTTTTTGAAGCCAAAGTTGCGAATGGTAATGGAGAACAAACACTAAGTCGTGACGTTTACCGACTTGGACGCCGGTTTGATTTTTACAGGATGCTTTCATTCTACTTCACCACAGTTGGTTTCTATTTCAGTAGTATGATAACGGTGCTCACGGTATATGTGTTCCTTTATGGGCGTCTTTATCTGGTATTGAGCGGATTAGAAAAAAACATCCTGCAAAGTGCAAGTGTACATGAGTCCAATGCCCTTGAGCAGGCACTAGCAGCCCAATCTGTTTTCCAGTTAGGTTTTCTGATGGTTCTACCAATGGTTATGGAAATTGGCCTAGAAAAAGGGTTTCGCACAGCCTTGGGTGATTTCATCATAATGCAGCTGCAGCTTGCCTCTGTTTTCTTCACGTTTCAACTGGGAACGAAAGCGCATTACTTTGGAAGGACGATTTTGCATGGAGGCTCTAAGTACCGAGCAACTGGTCGAGGTTTTGTTGTTTTCCATGCGAAGTTTGCAGAAAACTATAGACTATATTCGCGAAGCCATTTTGTTAAGGGACTGGAGTTGGTCATACTACTAGTTGTGTATCAAGTTTATGGAACTTCGTACCGTAGCTCAAGTACTTATATGTACATCACATTTTCAATGTGGTTCCTGGTGACTTCTTGGTTGTTTGCTCCATTTATATTCAACCCATCTGGGTTTGAGTGGCAAAAGACGGTGGACGATTGGACTGACTGGAAACGTTGGATGGGAAACCGCGGTGGCATAGGAATTGTTCTTGACAAAAGTTGGGAATCATGGTGGGATATAGAGCAAGAACATCTCAAGCACACAAATTTAAGAGGAAGGGTTCTAGAGATACTTCTCGCTCTGCGTTTTCTCCTTTACCAGTATGGAATTGTCTACCACCTCAACATCGCTCGCCGCCACACAACATTTCTGGTTTATGGACTCTCTTGGGCTATCTTGTTGTCAGTTCTTCTCGTGCTAAAGATGGTATCAATGGGTAGACGGAAGTTTGGTACAGATTTTCAGGTTATGTTCAGGATTCTCAAGGCACTTCTCTTCCTTGGTTTCTTGTCAGTCATGACTGTATTATTTGTCGTCTGCGGCCTCACGATCTCGGATCTCTTTGCCTCCATCCTCGCTTTCTTACCTACTGGCTGGGCTATTCTTCTTATCGGGCAAGCGCTGCGTAGCGTGTTCAAAGGATTAGGATTCTGGGACTCGGTGAAAGAGCTAGGGAGAGCTTACGAGTATATAATGGGGCTTGTGATATTCACACCCATTGCTGTCTTATCGTGGTTCCCATTTGTGTCTGAGTTCCAAACTAGATTACTGTTTAATCAAGCCTTCAGTCGCGGTCTGCAGATCTCTATGATTCTCGCCGGAAAGAAAGACAAAGAGACACCTTCCACCAAGTACCTAGGCCATACGGAAGAGTCCTTTGGACTTGAACATGATACAAATACCTTTAACCATTATTACCTTTGGACTTGA

Protein Analysis

1958

Amino Acids

228.03

Weight (kDa)

8.5

Isoelectric Point (pI)

42.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 57 - 185 1.2e-10 Vta1 like
FKS1_dom1 PF14288 340 - 450 3.6e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 859 - 1043 1.6e-67 Callose synthase 1 domain
Glucan_synthase PF02364 1050 - 1140 2.2e-28 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1139 - 1744 1.8e-206 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1214
AarI CACCTGC 1 cut(s) 1166
Acc36I ACCTGC 2 cut(s) 983, 1166
AccB1I GGYRCC 1 cut(s) 1554
AccB7I CCANNNNNTGG 1 cut(s) 1108
AccBSI CCGCTC 1 cut(s) 4578
AccI GTMKAC 4 cut(s) 2039, 4036, 5280, 5386
AccII CGCG 4 cut(s) 3942, 4912, 5136, 5739
AccIII TCCGGA 1 cut(s) 2829
AclI AACGTT 1 cut(s) 5119
AclWI GGATC 5 cut(s) 266, 279, 879, 1573, 5511
AcoI YGGCCR 6 cut(s) 41, 459, 1110, 1346, 4150, 4195
AcsI RAATTY 9 cut(s) 384, 1128, 1727, 2825, 2870, 3193, 3638, 4022, 5212
AcuI CTGAAG 5 cut(s) 1611, 1798, 2284, 3561, 5716
AcyI GRCGYC 2 cut(s) 4453, 4558
AfeI AGCGCT 1 cut(s) 5568
AfiI CCNNNNNNNGG 6 cut(s) 1108, 1157, 2311, 2998, 3445, 4636
AflII CTTAAG 2 cut(s) 1201, 4270
AflIII ACRYGT 4 cut(s) 1605, 3237, 3385, 4156
AgeI ACCGGT 1 cut(s) 1842
AhdI GACNNNNNGTC 1 cut(s) 1423
AhlI ACTAGT 2 cut(s) 11, 4951
AjiI CACGTC 1 cut(s) 4040
AjnI CCWGG 8 cut(s) 723, 1227, 2937, 2961, 3086, 3528, 3972, 5028
AjuI GAANNNNNNNTTGG 4 cut(s) 691, 723, 5808, 5840
AleI CACNNNNGTG 1 cut(s) 2464
AloI GAACNNNNNNTCC 2 cut(s) 279, 311
Alw21I GWGCWC 4 cut(s) 1183, 3619, 3625, 4533
Alw26I GTCTC 9 cut(s) 78, 1405, 1431, 1530, 2715, 2922, 3693, 3776, 5777
Alw44I GTGCAC 2 cut(s) 1179, 3615
AlwI GGATC 5 cut(s) 266, 279, 879, 1573, 5511
AlwNI CAGNNNCTG 1 cut(s) 1821
Ama87I CYCGRG 5 cut(s) 770, 875, 2309, 3225, 3908
Aor13HI TCCGGA 1 cut(s) 2829
Aor51HI AGCGCT 1 cut(s) 5568
ApaLI GTGCAC 2 cut(s) 1179, 3615
ApeKI GCWGC 9 cut(s) 55, 1739, 1818, 1895, 4646, 4753, 4756, 4759, 5569
ApoI RAATTY 9 cut(s) 384, 1128, 1727, 2825, 2870, 3193, 3638, 4022, 5212
ArsI GACNNNNNNTTYG 6 cut(s) 279, 311, 3968, 4000, 4414, 4446
AsiGI ACCGGT 1 cut(s) 1842
Asp700I GAANNNNTTC 3 cut(s) 2474, 3809, 4096
AspA2I CCTAGG 1 cut(s) 5803
AspLEI GCGC 2 cut(s) 4804, 5569
AspS9I GGNCC 4 cut(s) 1749, 2216, 2340, 3878
AsuC2I CCSGG 1 cut(s) 3876
AsuHPI GGTGA 9 cut(s) 1832, 2950, 3029, 3908, 3956, 4483, 4748, 5044, 5620
AsuNHI GCTAGC 1 cut(s) 2591
AvaI CYCGRG 5 cut(s) 770, 875, 2309, 3225, 3908
AvaII GGWCC 2 cut(s) 2216, 2340
AvrII CCTAGG 1 cut(s) 5803
AxyI CCTNAGG 2 cut(s) 542, 3360
BaeGI GKGCMC 2 cut(s) 1183, 3619
BalI TGGCCA 5 cut(s) 461, 1112, 1348, 4152, 4197
BamHI GGATCC 1 cut(s) 271
BanI GGYRCC 1 cut(s) 1554
BanII GRGCYC 3 cut(s) 1981, 3004, 3625
BauI CACGAG 4 cut(s) 87, 3618, 4331, 5360
BbsI GAAGAC 2 cut(s) 2538, 4061
Bbv12I GWGCWC 4 cut(s) 1183, 3619, 3625, 4533
BbvCI CCTCAGC 1 cut(s) 715
BbvI GCAGC 9 cut(s) 67, 1751, 1830, 1882, 4658, 4743, 4765, 4771, 5556
BcgI CGANNNNNNTGC 4 cut(s) 44, 78, 761, 795
BciT130I CCWGG 8 cut(s) 725, 1229, 2939, 2963, 3088, 3530, 3974, 5030
BciVI GTATCC 2 cut(s) 2236, 3753
BclI TGATCA 1 cut(s) 2505
BcnI CCSGG 1 cut(s) 3876
BcoDI GTCTC 9 cut(s) 78, 1405, 1431, 1530, 2715, 2922, 3693, 3776, 5777
BcuI ACTAGT 2 cut(s) 11, 4951
BfmI CTRYAG 8 cut(s) 627, 990, 1529, 3344, 3499, 4757, 4897, 5744
BfoI RGCGCY 1 cut(s) 5570
BfrI CTTAAG 2 cut(s) 1201, 4270
BfuAI ACCTGC 2 cut(s) 983, 1166
BfuI GTATCC 2 cut(s) 2236, 3753
BglI GCCNNNNNGGC 1 cut(s) 1748
BglII AGATCT 5 cut(s) 752, 1389, 2500, 3061, 5748
BlnI CCTAGG 1 cut(s) 5803
BlpI GCTNAGC 1 cut(s) 1980
BmcAI AGTACT 3 cut(s) 10, 1313, 4996
Bme1390I CCNGG 9 cut(s) 725, 1229, 2939, 2963, 3088, 3530, 3876, 3974, 5030
Bme18I GGWCC 2 cut(s) 2216, 2340
BmeRI GACNNNNNGTC 1 cut(s) 1423
BmeT110I CYCGRG 5 cut(s) 770, 875, 2309, 3225, 3908
BmgBI CACGTC 1 cut(s) 4040
BmgT120I GGNCC 4 cut(s) 1749, 2216, 2340, 3878
BmiI GGNNCC 9 cut(s) 273, 513, 1481, 1556, 2217, 2296, 3674, 5027, 5684
BmrFI CCNGG 9 cut(s) 725, 1229, 2939, 2963, 3088, 3530, 3876, 3974, 5030
BmrI ACTGGG 2 cut(s) 1167, 4799
BmsI GCATC 6 cut(s) 121, 838, 1067, 2808, 3208, 4464
BmtI GCTAGC 1 cut(s) 2595
BmuI ACTGGG 2 cut(s) 1167, 4799
BpiI GAAGAC 2 cut(s) 2538, 4061
BplI GAGNNNNNCTC 2 cut(s) 3495, 3527
BpmI CTGGAG 5 cut(s) 2571, 2921, 3109, 3938, 4955
Bpu10I CCTNAGC 1 cut(s) 715
Bpu1102I GCTNAGC 1 cut(s) 1980
BpuEI CTTGAG 5 cut(s) 501, 4652, 4975, 5186, 5414
BpuMI CCSGG 1 cut(s) 3876
Bsa29I ATCGAT 1 cut(s) 855
BsaBI GATNNNNATC 1 cut(s) 2499
BsaHI GRCGYC 2 cut(s) 4453, 4558
BsaI GGTCTC 1 cut(s) 3693
BsaJI CCNNGG 6 cut(s) 1123, 2961, 4731, 5134, 5446, 5803
BsaWI WCCGGW 5 cut(s) 448, 1183, 1842, 2829, 3702
Bsc4I CCNNNNNNNGG 6 cut(s) 1108, 1157, 2311, 2998, 3445, 4636
Bse118I RCCGGY 2 cut(s) 1842, 4455
Bse21I CCTNAGG 2 cut(s) 542, 3360
Bse3DI GCAATG 7 cut(s) 171, 682, 1147, 2445, 2576, 3749, 5664
Bse8I GATNNNNATC 1 cut(s) 2499
BseAI TCCGGA 1 cut(s) 2829
BseBI CCWGG 8 cut(s) 725, 1229, 2939, 2963, 3088, 3530, 3974, 5030
BseCI ATCGAT 1 cut(s) 855
BseDI CCNNGG 6 cut(s) 1123, 2961, 4731, 5134, 5446, 5803
BseJI GATNNNNATC 1 cut(s) 2499
BseLI CCNNNNNNNGG 6 cut(s) 1108, 1157, 2311, 2998, 3445, 4636
BseMI GCAATG 7 cut(s) 171, 682, 1147, 2445, 2576, 3749, 5664
BseRI GAGGAG 5 cut(s) 347, 350, 467, 3794, 4251
BseSI GKGCMC 2 cut(s) 1183, 3619
BseX3I CGGCCG 1 cut(s) 41
BseXI GCAGC 9 cut(s) 67, 1751, 1830, 1882, 4658, 4743, 4765, 4771, 5556
BseYI CCCAGC 2 cut(s) 1821, 5541
Bsh1236I CGCG 4 cut(s) 3942, 4912, 5136, 5739
Bsh1285I CGRYCG 3 cut(s) 44, 1501, 1847
BshNI GGYRCC 1 cut(s) 1554
BshTI ACCGGT 1 cut(s) 1842
BshVI ATCGAT 1 cut(s) 855
BsiEI CGRYCG 3 cut(s) 44, 1501, 1847
BsiHKAI GWGCWC 4 cut(s) 1183, 3619, 3625, 4533
BsiHKCI CYCGRG 5 cut(s) 770, 875, 2309, 3225, 3908
BsiSI CCGG 8 cut(s) 449, 1184, 1843, 2830, 3703, 3876, 4456, 5766
BslFI GGGAC 3 cut(s) 1175, 4944, 5615
BslI CCNNNNNNNGG 6 cut(s) 1108, 1157, 2311, 2998, 3445, 4636
BsmAI GTCTC 9 cut(s) 78, 1405, 1431, 1530, 2715, 2922, 3693, 3776, 5777
BsmFI GGGAC 3 cut(s) 1175, 4944, 5615
BsmI GAATGC 3 cut(s) 1088, 1196, 2013
Bso31I GGTCTC 1 cut(s) 3693
BsoBI CYCGRG 5 cut(s) 770, 875, 2309, 3225, 3908
Bsp1286I GDGCHC 6 cut(s) 1183, 1981, 3004, 3619, 3625, 4533
Bsp13I TCCGGA 1 cut(s) 2829
Bsp1407I TGTACA 2 cut(s) 4612, 5004
Bsp1720I GCTNAGC 1 cut(s) 1980
Bsp19I CCATGG 1 cut(s) 1123
Bsp68I TCGCGA 1 cut(s) 4912
BspDI ATCGAT 1 cut(s) 855
BspEI TCCGGA 1 cut(s) 2829
BspFNI CGCG 4 cut(s) 3942, 4912, 5136, 5739
BspHI TCATGA 2 cut(s) 3204, 5463
BspLI GGNNCC 9 cut(s) 273, 513, 1481, 1556, 2217, 2296, 3674, 5027, 5684
BspMAI CTGCAG 4 cut(s) 631, 994, 4761, 5748
BspMI ACCTGC 2 cut(s) 983, 1166
BspOI GCTAGC 1 cut(s) 2595
BspPI GGATC 5 cut(s) 266, 279, 879, 1573, 5511
BspQI GCTCTTC 1 cut(s) 1682
BspT107I GGYRCC 1 cut(s) 1554
BspTI CTTAAG 2 cut(s) 1201, 4270
BspTNI GGTCTC 1 cut(s) 3693
BsrBI CCGCTC 1 cut(s) 4578
BsrDI GCAATG 7 cut(s) 171, 682, 1147, 2445, 2576, 3749, 5664
BsrFI RCCGGY 2 cut(s) 1842, 4455
BsrGI TGTACA 2 cut(s) 4612, 5004
BssAI RCCGGY 2 cut(s) 1842, 4455
BssECI CCNNGG 6 cut(s) 1123, 2961, 4731, 5134, 5446, 5803
BssNAI GTATAC 1 cut(s) 2040
BssNI GRCGYC 2 cut(s) 4453, 4558
BssSI CACGAG 4 cut(s) 87, 3618, 4331, 5360
BssT1I CCWWGG 4 cut(s) 1123, 4731, 5446, 5803
Bst1107I GTATAC 1 cut(s) 2040
Bst2BI CACGAG 4 cut(s) 87, 3618, 4331, 5360
Bst2UI CCWGG 8 cut(s) 725, 1229, 2939, 2963, 3088, 3530, 3974, 5030
Bst6I CTCTTC 9 cut(s) 19, 336, 362, 860, 1682, 3984, 4311, 5447, 5811
BstACI GRCGYC 2 cut(s) 4453, 4558
BstAFI CTTAAG 2 cut(s) 1201, 4270
BstAPI GCANNNNNTGC 3 cut(s) 224, 665, 5569
BstAUI TGTACA 2 cut(s) 4612, 5004
BstDSI CCRYGG 2 cut(s) 1123, 5134
BstENI CCTNNNNNAGG 1 cut(s) 4634
BstFNI CGCG 4 cut(s) 3942, 4912, 5136, 5739
BstH2I RGCGCY 1 cut(s) 5570
BstHHI GCGC 2 cut(s) 4804, 5569
BstMAI GTCTC 9 cut(s) 78, 1405, 1431, 1530, 2715, 2922, 3693, 3776, 5777
BstMCI CGRYCG 3 cut(s) 44, 1501, 1847
BstNI CCWGG 8 cut(s) 725, 1229, 2939, 2963, 3088, 3530, 3974, 5030
BstNSI RCATGY 3 cut(s) 1951, 2144, 3241
BstSCI CCNGG 9 cut(s) 723, 1227, 2937, 2961, 3086, 3528, 3874, 3972, 5028
BstSFI CTRYAG 8 cut(s) 627, 990, 1529, 3344, 3499, 4757, 4897, 5744
BstSLI GKGCMC 2 cut(s) 1183, 3619
BstUI CGCG 4 cut(s) 3942, 4912, 5136, 5739
BstV1I GCAGC 9 cut(s) 67, 1751, 1830, 1882, 4658, 4743, 4765, 4771, 5556
BstV2I GAAGAC 2 cut(s) 2538, 4061
BstX2I RGATCY 9 cut(s) 271, 752, 871, 1389, 1565, 2500, 3061, 5503, 5748
BstYI RGATCY 9 cut(s) 271, 752, 871, 1389, 1565, 2500, 3061, 5503, 5748
BstZ17I GTATAC 1 cut(s) 2040
BstZI CGGCCG 1 cut(s) 41
Bsu15I ATCGAT 1 cut(s) 855
Bsu36I CCTNAGG 2 cut(s) 542, 3360
BsuI GTATCC 2 cut(s) 2236, 3753
BsuTUI ATCGAT 1 cut(s) 855
BtgI CCRYGG 2 cut(s) 1123, 5134
BtgZI GCGATG 2 cut(s) 2188, 5278
BtrI CACGTC 1 cut(s) 4040
BtsI GCAGTG 1 cut(s) 2700
BtsIMutI CAGTG 7 cut(s) 513, 1380, 1655, 2700, 2860, 3537, 3885
BtuMI TCGCGA 1 cut(s) 4912
BveI ACCTGC 2 cut(s) 983, 1166
CaiI CAGNNNCTG 1 cut(s) 1821
CciI TCATGA 2 cut(s) 3204, 5463
CciNI GCGGCCGC 1 cut(s) 41
CfoI GCGC 2 cut(s) 4804, 5569
Cfr10I RCCGGY 2 cut(s) 1842, 4455
Cfr13I GGNCC 4 cut(s) 1749, 2216, 2340, 3878
Cfr42I CCGCGG 1 cut(s) 5137
ClaI ATCGAT 1 cut(s) 855
CseI GACGC 2 cut(s) 4461, 4547
CsiI ACCWGGT 1 cut(s) 1227
CspAI ACCGGT 1 cut(s) 1842
CspCI CAANNNNNGTGG 2 cut(s) 3874, 3909
DraI TTTAAA 3 cut(s) 501, 2350, 3636
DriI GACNNNNNGTC 1 cut(s) 1423
EaeI YGGCCR 6 cut(s) 41, 459, 1110, 1346, 4150, 4195
EagI CGGCCG 1 cut(s) 41
Eam1104I CTCTTC 9 cut(s) 19, 336, 362, 860, 1682, 3984, 4311, 5447, 5811
Eam1105I GACNNNNNGTC 1 cut(s) 1423
EarI CTCTTC 9 cut(s) 19, 336, 362, 860, 1682, 3984, 4311, 5447, 5811
EciI GGCGGA 1 cut(s) 3428
Ecl136II GAGCTC 1 cut(s) 3623
EclXI CGGCCG 1 cut(s) 41
Eco130I CCWWGG 4 cut(s) 1123, 4731, 5446, 5803
Eco24I GRGCYC 3 cut(s) 1981, 3004, 3625
Eco31I GGTCTC 1 cut(s) 3693
Eco32I GATATC 1 cut(s) 4282
Eco47I GGWCC 2 cut(s) 2216, 2340
Eco47III AGCGCT 1 cut(s) 5568
Eco52I CGGCCG 1 cut(s) 41
Eco53kI GAGCTC 1 cut(s) 3623
Eco57I CTGAAG 5 cut(s) 1611, 1798, 2284, 3561, 5716
Eco81I CCTNAGG 2 cut(s) 542, 3360
Eco88I CYCGRG 5 cut(s) 770, 875, 2309, 3225, 3908
EcoICRI GAGCTC 1 cut(s) 3623
EcoNI CCTNNNNNAGG 1 cut(s) 4634
EcoRI GAATTC 2 cut(s) 1128, 2825
EcoRII CCWGG 8 cut(s) 723, 1227, 2937, 2961, 3086, 3528, 3972, 5028
EcoRV GATATC 1 cut(s) 4282
EcoT14I CCWWGG 4 cut(s) 1123, 4731, 5446, 5803
EcoT22I ATGCAT 4 cut(s) 250, 2013, 2142, 2688
EcoT38I GRGCYC 3 cut(s) 1981, 3004, 3625
ErhI CCWWGG 4 cut(s) 1123, 4731, 5446, 5803
FalI AAGNNNNNCTT 4 cut(s) 211, 243, 4084, 4116
FaqI GGGAC 3 cut(s) 1175, 4944, 5615
FauI CCCGC 1 cut(s) 2484
FauNDI CATATG 5 cut(s) 357, 1046, 1168, 2210, 3646
FbaI TGATCA 1 cut(s) 2505
FblI GTMKAC 4 cut(s) 2039, 4036, 5280, 5386
FriOI GRGCYC 3 cut(s) 1981, 3004, 3625
GlaI GCGC 2 cut(s) 4803, 5568
GsaI CCCAGC 2 cut(s) 1825, 5545
GsuI CTGGAG 5 cut(s) 2571, 2921, 3109, 3938, 4955
HaeII RGCGCY 1 cut(s) 5570
HapII CCGG 8 cut(s) 449, 1184, 1843, 2830, 3703, 3876, 4456, 5766
HgaI GACGC 2 cut(s) 4461, 4547
HhaI GCGC 2 cut(s) 4804, 5569
Hin1I GRCGYC 2 cut(s) 4453, 4558
Hin6I GCGC 2 cut(s) 4802, 5567
HinP1I GCGC 2 cut(s) 4802, 5567
HincII GTYRAC 5 cut(s) 1611, 2113, 2650, 2977, 4037
HindII GTYRAC 5 cut(s) 1611, 2113, 2650, 2977, 4037
HindIII AAGCTT 1 cut(s) 464
HpaI GTTAAC 1 cut(s) 2977
HpaII CCGG 8 cut(s) 449, 1184, 1843, 2830, 3703, 3876, 4456, 5766
HphI GGTGA 9 cut(s) 1832, 2950, 3029, 3908, 3956, 4483, 4748, 5044, 5620
Hpy99I CGWCG 3 cut(s) 3174, 3177, 4041
Hsp92I GRCGYC 2 cut(s) 4453, 4558
HspAI GCGC 2 cut(s) 4802, 5567
Kpn2I TCCGGA 1 cut(s) 2829
Ksp22I TGATCA 1 cut(s) 2505
KspAI GTTAAC 1 cut(s) 2977
KspI CCGCGG 1 cut(s) 5137
LguI GCTCTTC 1 cut(s) 1682
LmnI GCTCC 6 cut(s) 181, 826, 1936, 2294, 3226, 5056
Lsp1109I GCAGC 9 cut(s) 67, 1751, 1830, 1882, 4658, 4743, 4765, 4771, 5556
LweI GCATC 6 cut(s) 121, 838, 1067, 2808, 3208, 4464
MabI ACCWGGT 1 cut(s) 1227
MbiI CCGCTC 1 cut(s) 4578
MfeI CAATTG 2 cut(s) 135, 2220
MflI RGATCY 9 cut(s) 271, 752, 871, 1389, 1565, 2500, 3061, 5503, 5748
MhlI GDGCHC 6 cut(s) 1183, 1981, 3004, 3619, 3625, 4533
MlsI TGGCCA 5 cut(s) 461, 1112, 1348, 4152, 4197
MluNI TGGCCA 5 cut(s) 461, 1112, 1348, 4152, 4197
MlyI GAGTC 7 cut(s) 965, 3067, 3460, 4628, 5322, 5597, 5828
MmeI TCCRAC 3 cut(s) 299, 2827, 5102
Mox20I TGGCCA 5 cut(s) 461, 1112, 1348, 4152, 4197
Mph1103I ATGCAT 4 cut(s) 250, 2013, 2142, 2688
MroI TCCGGA 1 cut(s) 2829
MroXI GAANNNNTTC 3 cut(s) 2474, 3809, 4096
MscI TGGCCA 5 cut(s) 461, 1112, 1348, 4152, 4197
MslI CAYNNNNRTG 7 cut(s) 117, 857, 1383, 2464, 3209, 3965, 4748
Msp20I TGGCCA 5 cut(s) 461, 1112, 1348, 4152, 4197
MspA1I CMGCKG 4 cut(s) 604, 1821, 4756, 5136
MspCI CTTAAG 2 cut(s) 1201, 4270
MspI CCGG 8 cut(s) 449, 1184, 1843, 2830, 3703, 3876, 4456, 5766
MspR9I CCNGG 9 cut(s) 725, 1229, 2939, 2963, 3088, 3530, 3876, 3974, 5030
MunI CAATTG 2 cut(s) 135, 2220
Mva1269I GAATGC 3 cut(s) 1088, 1196, 2013
MvaI CCWGG 8 cut(s) 725, 1229, 2939, 2963, 3088, 3530, 3974, 5030
MvnI CGCG 4 cut(s) 3942, 4912, 5136, 5739
NciI CCSGG 1 cut(s) 3876
NcoI CCATGG 1 cut(s) 1123
NdeI CATATG 5 cut(s) 357, 1046, 1168, 2210, 3646
NheI GCTAGC 1 cut(s) 2591
NlaIV GGNNCC 9 cut(s) 273, 513, 1481, 1556, 2217, 2296, 3674, 5027, 5684
NmeAIII GCCGAG 1 cut(s) 3722
NotI GCGGCCGC 1 cut(s) 41
NruI TCGCGA 1 cut(s) 4912
NsiI ATGCAT 4 cut(s) 250, 2013, 2142, 2688
NspI RCATGY 3 cut(s) 1951, 2144, 3241
OliI CACNNNNGTG 1 cut(s) 2464
PaeR7I CTCGAG 2 cut(s) 770, 875
PagI TCATGA 2 cut(s) 3204, 5463
PaqCI CACCTGC 1 cut(s) 1166
PciI ACATGT 1 cut(s) 3237
PciSI GCTCTTC 1 cut(s) 1682
PcsI WCGNNNNNNNCGW 3 cut(s) 295, 1400, 2151
PctI GAATGC 3 cut(s) 1088, 1196, 2013
PdmI GAANNNNTTC 3 cut(s) 2474, 3809, 4096
PflMI CCANNNNNTGG 1 cut(s) 1108
PfoI TCCNGGA 3 cut(s) 2937, 3086, 3528
PinAI ACCGGT 1 cut(s) 1842
PleI GAGTC 7 cut(s) 965, 3067, 3460, 4627, 5322, 5597, 5827
PpsI GAGTC 7 cut(s) 965, 3067, 3460, 4627, 5322, 5597, 5827
PscI ACATGT 1 cut(s) 3237
PsiI TTATAA 1 cut(s) 1214
Psp124BI GAGCTC 1 cut(s) 3625
Psp1406I AACGTT 1 cut(s) 5119
Psp6I CCWGG 8 cut(s) 723, 1227, 2937, 2961, 3086, 3528, 3972, 5028
PspFI CCCAGC 2 cut(s) 1821, 5541
PspGI CCWGG 8 cut(s) 723, 1227, 2937, 2961, 3086, 3528, 3972, 5028
PspN4I GGNNCC 9 cut(s) 273, 513, 1481, 1556, 2217, 2296, 3674, 5027, 5684
PspPI GGNCC 4 cut(s) 1749, 2216, 2340, 3878
PstI CTGCAG 4 cut(s) 631, 994, 4761, 5748
PstNI CAGNNNCTG 1 cut(s) 1821
PsuI RGATCY 9 cut(s) 271, 752, 871, 1389, 1565, 2500, 3061, 5503, 5748
PvuII CAGCTG 2 cut(s) 1821, 4756
RruI TCGCGA 1 cut(s) 4912
RseI CAYNNNNRTG 7 cut(s) 117, 857, 1383, 2464, 3209, 3965, 4748
SacI GAGCTC 1 cut(s) 3625
SacII CCGCGG 1 cut(s) 5137
SalI GTCGAC 1 cut(s) 4035
SapI GCTCTTC 1 cut(s) 1682
Sau96I GGNCC 4 cut(s) 1749, 2216, 2340, 3878
SbfI CCTGCAGG 1 cut(s) 994
ScaI AGTACT 3 cut(s) 10, 1313, 4996
SchI GAGTC 7 cut(s) 965, 3067, 3460, 4628, 5322, 5597, 5828
ScrFI CCNGG 9 cut(s) 725, 1229, 2939, 2963, 3088, 3530, 3876, 3974, 5030
SdaI CCTGCAGG 1 cut(s) 994
SduI GDGCHC 6 cut(s) 1183, 1981, 3004, 3619, 3625, 4533
SexAI ACCWGGT 1 cut(s) 1227
SfaNI GCATC 6 cut(s) 121, 838, 1067, 2808, 3208, 4464
SfcI CTRYAG 8 cut(s) 627, 990, 1529, 3344, 3499, 4757, 4897, 5744
Sfr274I CTCGAG 2 cut(s) 770, 875
Sfr303I CCGCGG 1 cut(s) 5137
SgrBI CCGCGG 1 cut(s) 5137
SinI GGWCC 2 cut(s) 2216, 2340
SlaI CTCGAG 2 cut(s) 770, 875
SmiMI CAYNNNNRTG 7 cut(s) 117, 857, 1383, 2464, 3209, 3965, 4748
SmlI CTYRAG 9 cut(s) 516, 770, 875, 1201, 4270, 4631, 4990, 5201, 5429
SmoI CTYRAG 9 cut(s) 516, 770, 875, 1201, 4270, 4631, 4990, 5201, 5429
SpeI ACTAGT 2 cut(s) 11, 4951
Sse8387I CCTGCAGG 1 cut(s) 994
SspI AATATT 1 cut(s) 1888
SstI GAGCTC 1 cut(s) 3625
StyD4I CCNGG 9 cut(s) 723, 1227, 2937, 2961, 3086, 3528, 3874, 3972, 5028
StyI CCWWGG 4 cut(s) 1123, 4731, 5446, 5803
TaqII GACCGA 2 cut(s) 54, 1515
TatI WGTACW 6 cut(s) 8, 1311, 3495, 4612, 4994, 5004
TauI GCSGC 6 cut(s) 43, 46, 49, 659, 5304, 5490
TscAI CASTG 7 cut(s) 513, 1387, 1662, 2700, 2860, 3544, 3892
TseI GCWGC 9 cut(s) 55, 1739, 1818, 1895, 4646, 4753, 4756, 4759, 5569
TspGWI ACGGA 3 cut(s) 3735, 5404, 5828
TspRI CASTG 7 cut(s) 513, 1387, 1662, 2700, 2860, 3544, 3892
Van91I CCANNNNNTGG 1 cut(s) 1108
Vha464I CTTAAG 2 cut(s) 1201, 4270
VneI GTGCAC 2 cut(s) 1179, 3615
VpaK11BI GGWCC 2 cut(s) 2216, 2340
XagI CCTNNNNNAGG 1 cut(s) 4634
XapI RAATTY 9 cut(s) 384, 1128, 1727, 2825, 2870, 3193, 3638, 4022, 5212
XbaI TCTAGA 1 cut(s) 5230
XceI RCATGY 3 cut(s) 1951, 2144, 3241
XcmI CCANNNNNNNNNTGG 2 cut(s) 1427, 1793
XhoI CTCGAG 2 cut(s) 770, 875
XmaJI CCTAGG 1 cut(s) 5803
XmiI GTMKAC 4 cut(s) 2039, 4036, 5280, 5386
XmnI GAANNNNTTC 3 cut(s) 2474, 3809, 4096
XspI CTAG 3 cut(s) 12, 329, 900
ZrmI AGTACT 3 cut(s) 10, 1313, 4996
Zsp2I ATGCAT 4 cut(s) 250, 2013, 2142, 2688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.