FvH4_6g38500

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009173
Physical Location & Seq
Reverse (-)
30421969 .. 30436289
14321 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g38500.t1

Sequence Viewer

Length: 5796 bp
ATGGCGAGCTCGAGCGGGACCAAGGCGGTGGACTCGTCGGGGCCGCAGAGGTCGCTGTCGAGGGGGATGACGAGAATGTCTACTAGAGTTGTGGACTTTCCGACGGAGGGCGACGATGCTCTGGACAGTGGAGTTGTGCCCTCCTGCCTTGCTTCTATTGCTCCCATTTTCAGGGTTGCCAATGAGATTGAGAAGGACAACCCTAGGGTTGCTTATCTATGCCGCTTCCATGGATTTGAAAAGGCGCATAAAATGGATCCAACATCAAGTGGACGGGGAGTTCGTCAGTTTAAGACTCATCTTCTGCACAGACTCGAAAAGGACGAACGAGATACAAAACATCAGCTTGCTAAATCTGATACAAACGAAATCAAGTTGTACTACCACGAATTCTATGAAAAAAATATCAAAGATGGAGAATATACAAAGAAACCGGAAGAGATGGCCAGGAATTGTCAGATTGCAACTGTACTGTACGATGTGCTTAGGACAGTGGTACCTGCCAATCAGATAGACAAACAGAGCCAGAAAATTGCTGAAGACGTCAAGAGAAAAAGGGAGCAGTATGTAAACTATAACATTCTTCCGTTATATACTGTCGGGGTTAAACCAGCAGTTATGGAACTTCCTGAGATCAAAGCAGCGCTCCGTGCTCTACAGAATGTGGATAATCTTCCAATGCCAAGAATGCATTTTAAACCCACGAATCCTGATGACAAGTCGACAATGCCTACAGAGAGGATTAAACCTGTGAATGATATACTTGATTGGCTTTCCGCTATTTTTGGGTTTCAGAAAGGAAATGTGGCAAATCAGAGGGAACATCTAATTTTGCTACTTGCCAACATTGATGTAAGACATAGAAGTCTTGAGAACTACACTGAGTTGAATAGTGCCACTGTACAACATTTGATGGAGAAATTTTTTAAGAACTACCGCTCGTGGTATAACTATCTGCACTCTAAATCAAATCTTCGGTTCCCTGAAGGTTTTCACAGACAACAATTGGAACTTATATACATTGCGCTCTATCTGCTCATATGGGGTGAAGCTTCAAATGTCCGATTCATGCCTGAATGCTTGTGCTACATTTTCCATAATATGGCAAATGAAGTCTATGGAATTTTAAATAGCAATGCTCATCATGTTAGTGGAGAAACATACCAAACAGTAGCACATGATGAAGAATCTTTTCTGAGGGATGTTATAGCTCCTATCTACGGGGTTTTGTTCAAGGAAGCAAAGAGAAACAAATTGGGCAAGGCAAGTCATTCAAGATGGAGAAATTATGATGATCTGAATGAATACTTCTGGTCGGACAAATGTTTTAAGCTAGGGTGGCCGATGGATCCTAAAGCAGATTTCTTTAGGCATTCGGATGTGAGACAACCAGCAAATGAGAGGACAAATCAAGCTACTGGTGGTAGGAGGAAGCCTAAAACAAATTTTGTAGAAGTTCGAACCTTTTTGCATCTCTACAGAAGTTTTGATCGAATGTGGATATTCTTCATATTGGCTTTACAGGCTATGATTATTGTTGCATGGGGTCCTTCTGGATATGTCACTTCAATATTTGATGCGGCTGTCTTCAAAAGTGTGTTAAGCATCTTCATGACATACGCTTTCCTGAATTTATTACAAGCCACTTTGGACATAATTCTCAGTTGGAATGCATGGAAGAGCTTGAAATTCACTCAAATACTACGATACCTTTTGAAATTTGCAGTAGCTGTTGTCTGGGCTGTGGTGCTGCCTATTGGTTATTCAAGTTCTATGCAGAATCCTACAGGGCTTCTGAAATTCTTCAGTAGTTGGACTCGGGATTGGCGTAATCAATCATTTTATAATTATGCTGTTGCAATCTATATGGTTCCTAATATATTGGCTGCTATGCTATTTTTTCTTCCACCTTTACGGAGGCACATAGAGCGTTCCAACTGGCGCATTGTCACTCTTTTTATGTGGTGGGCTCAGCCAAAACTGTATGTCGGAAGAGGAATGCATGAGGATGTCTTCTCACTGCTGAAATATACATTGTTCTGGATCATGCTGCTAATCAGCAAGCTTGCATTCAGCTATTATGTGGAGATATCGCCGCTTGTTGCACCAACAAAGTTGATAATGGATATGTCTATCACTAATTATCAATGGCATGAGTTCTTTCCTAATGCAACTCATAATATTGGTGTTGTCATTGCAATATGGGCTCCAATTGTCTTAGTATACTTTATGGATGCACAAATATGGTATGCAATATTTTCTACTCTGTTCGGTGGGATTCACGGAGCCTTCAGCCATTTGGGGGAGATACGAACACTTGGGATGTTGCGGTCCAGATTTGAGTCTGTGCCTTCAGCTTTCAGTGATCGGCTTATGCCATCATCAGAAATTAAGGATGCCAAAAAGAAGAAACACTTGGTGGACGAAGGACTTGTTCGAAGGAACATTGCTAACTTCTCTCAGGTCTGGAATGAATTTATAAATTCTATGCGAATGGAAGACCTGATCAGCAATAGGGATAGAGATCTGCTGCTTGTACCGTATTCTTCAAGTGATGTTTCTGTTGTCCAGTGGCCCCCTTTCTTGCTTGCTAGCAAGATTCCGATAGCATTAGACATGGCAAAGGATTTTACGGGGAAGTCGGATGAGGATTTATTCAAGAAGATTATGAATGATGATTATATGTACTCGGCAGTGATTGAATGCTACGAGACACTCAGGGATATTATATTTGGCCTTCTTGAAGATGAGGCCGATAAGATGATTGTAAGGCAGATATGTTATGAAGTAGACAGCAGTATACAGCAGCAGAAGTTTTTGTTTAATTTCCGGATGAGTGGGCTGCCATTTCTTAGTGAGAGGTTGGAGAAGTTTCTTAAACTCTTGCTAGCTGAAGATGAGAATGTCGATAATTACTTGCGTCAGATAATCAATGTCCTTCAAGATATCATGGAGATTATAACACAGGATGTAATGATGAATGGCCATGAAATTCTTGAAGAAGCTCACTACATAGAGGGTCAAAATGTCAAGAAGGAGCAAAGGTTTGAAAAAATCAACATATACCTTGCACAAAATCCATCTTGGAGAGAGAAGGTTGTTAGGCTTCATTTGCTTTTGACCGTCAAGGAATCTGCCATCAATGTGCCCCAAAACTTGGATGCTCGCAGGCGCATTACTTTCTTTGCGAACTCTCTATTCATGAACATGCCAAATGCTCCCAAAGTTCGTGACATGCTCTCCTTTAGTGTTCTGACTCCTTATTACAAAGAAGATGTTCTGTATTCTGATGATGAACTTAACAAGGAAAATGAGGATGGAATCTCAATCTTGTTCTACCTGCAGAAGATATATCCTGATGAATGGACAAATTTCCAGGACCGCATAAATGAACATAAGAATGAGTACTCTGACAAGGACAAGTCGGAGTTAATTCGCCAGTGGGTATCTTACAGGGGACAGACACTTTCTAGAACAGTGAGAGGAATGATGTACTATAGGAAGGCTCTAGACCTTCAATGTATCCTGGAAACAGCAGGAGATGGTGGTGTTTATGGAGGCTACCCAGCCCAGATATTAAGTGAAATTGATGAGAAGGATATTCTTGATCGGGCACAAGCTCTGGCAGATTTGAAGTTCACTTATGTAGTTTCTTGTCAAATCTACGGTGAACAGAAGAAATCCAGTGAAAGTCGTGACAAAAGTTGTTACACAAATATTCTCAAGCTGATGTTAACGTATCCTTCTCTACGGGTTGCTTACATAGATACAAGAGAGGAGACAGTGAATGGAAAATCCCAAAAGATTTATTTTTCTGTGCTGGTCAAGGGAGGGGACAAATGGGATGAGGAAATATACCGCATCAAGCTTCCAGGTCCTCCAACAGTAATTGGTGAAGGAAAACCTGAAAATCAAAACCATGCCATCGTCTTTACTCGTGGAGAAGCCCTGCAAACCATTGACATGAATCAGGATAATTATTTTGAGGAAGCTTTCAAAATGAGAAATGTATTGGAGGAATTCCGAAAGCCCCGACGGGGGCAGCGGAAACCTACGATATTGGGTCTAAGGGAACATATTTTTACTGGAAGTGTTTCATCACTTGCTTGGTTCATGTCCAATCAAGAAACTAGTTTTGTCACTATAGGCCAACGAATTTTGGCAAATCCACTGAGGGTACGGTTTCATTATGGCCATCCGGATATTTTTGACAGACTCTTCCACATAACAAGGGGTGGCATAAGCAAAGCATCGAAAGTAATTAACTTAAGCGAGGATATATTTTCAGGTTACAATTCTGTTCTGCGTGGGGGATTTATAACACATCATGAGTATATCCAAGTAGGCAAGGGGCGTGATGTTGGAATGAATCAAATATCACTTTTCGAGGCTAAAGTTGCAAATGGGAATGGAGAGCAGACACTTAGCCGTGATGTTTATAGGCTTGGGCGTCGGTTTGATTTCTATAGAATGCTGTCCTTTTACTTCACAACAGTTGGGTTCTATTTTAGTAGCATGGTAACTGTGCTGACTGTCTATGTGTTTTTGTATGGACGTCTATATATGGTTATGAGTGGATTGGAAAGTGAGATCCTGGATAGCCCAGCCATACATCAGACCAAGGCTTTTGAAGAAGCTCTGGCTACACAGTCTGTCTTTCAACTGGGGTTGTTGCTGGTCCTCCCCATGGTTATGGAAATTGGTCTGGAGAAAGGATTTCGCACTGCCTTGGGTGATTTCATCATCATGCAGCTGCAGCTGGCTTCTGTTTTCTTCACTTTCCAACTAGGAACAAAAGCACATTATTACGGAAGGACAATCTTACATGGTGGATCTAAATATAGAGCAACTGGCCGTGGTTTTGTGGTTTTTCATGCAAAGTTTGCCGATAACTACAGGCTGTACTCAAGAAGTCACTTTGTGAAAGGGTTGGAGCTGTTGATTCTGCTGATTGTATATGGAGTCTACGGGAATGCTTATAGGAGTTCAAATCTGTATTTTTTCATCACATTCTCCATGTGGTTTCTGGTTGCTTCCTGGTTGTTTGCTCCATTCATCTTTAATCCGTCCAGCTTTGACTGGCAAAAAACTGTAGATGATTGGACTGATTGGAAGAGGTGGATGGGAAATCGTGGTGGTATTGGGATCTCACCTGACAAAAGTTGGGAATCATGGTGGGATGAAGAGCAGGAACACCTTAAGCACACTATTATCCGTGGAAGAATTCTTGAGATAATGCTTGCATTTCGCTTCTTCTTTTACCAATATGGGATTGTTTACCACCTCGATATAGCTCATTTCAGCAAGAGTTTACTGGTTTATGGACTTTCTTGGGTGGTTATGGTAACAGTGCTTCTCGTCTTAAAGATGGTGTCAATGGGTAGACGAAGATTTGGCACCGACTTTCAGCTCATGTTCAGAATTCTGAAAGCACTTCTTTTCCTTGGTTTCATGTCGGTCATGACTGTACTATTTGTTGTCTGTGGGCTTACTATTTCAGATCTGTTTGCCTCTATCCTTGCCTTCTTGCCCACAGGGTGGGCACTTCTTCTTATCGGGCAAGTATGCAGGCCAATGCTTAAGGGAGTAGGATTCTGGGAATCAATCAAGGAGCTGGGAAGAGCATATGATTATATAATGGGAGTAGTAGTCTTCGCGCCTGTTGCCATTTTGTCATGGTTCCCATTTGTCTCAGAGTTCCAAACACGGTTGCTCTTCAACCAAGCATTCAGTCGAGGCCTACAAATTTCTATGATTCTTGCCGGAAGGAAGGATAAGACGACGGCTTCAAGCTAG

Protein Analysis

1932

Amino Acids

223.99

Weight (kDa)

8.87

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 52 - 179 9.6e-12 Vta1 like
FKS1_dom1 PF14288 337 - 448 5.7e-42 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 859 - 1042 3.3e-70 Callose synthase 1 domain
Glucan_synthase PF02364 1049 - 1140 6.5e-29 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1142 - 1743 3.9e-202 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1845, 2480, 2960, 4319
AasI GACNNNNNNGTC 2 cut(s) 76, 864
AatII GACGTC 2 cut(s) 546, 4558
Acc36I ACCTGC 2 cut(s) 508, 3348
Acc65I GGTACC 1 cut(s) 496
AccB1I GGYRCC 2 cut(s) 496, 5396
AccB7I CCANNNNNTGG 3 cut(s) 1102, 3157, 5538
AccBSI CCGCTC 2 cut(s) 15, 939
AccI GTMKAC 7 cut(s) 80, 722, 2223, 2790, 2800, 4965, 5383
AccII CGCG 1 cut(s) 5657
AccIII TCCGGA 2 cut(s) 2829, 4198
AclWI GGATC 8 cut(s) 251, 264, 1343, 1356, 2051, 4585, 4840, 5153
AcoI YGGCCR 5 cut(s) 444, 1340, 2983, 4192, 4852
AcuI CTGAAG 6 cut(s) 558, 1005, 1789, 2275, 2337, 2913
AcyI GRCGYC 3 cut(s) 543, 4450, 4555
AdeI CACNNNGTG 3 cut(s) 2419, 4921, 5538
AfeI AGCGCT 1 cut(s) 645
AflII CTTAAG 3 cut(s) 4267, 5198, 5579
AhlI ACTAGT 1 cut(s) 4130
AjnI CCWGG 6 cut(s) 446, 3375, 3525, 3871, 4593, 5036
AjuI GAANNNNNNNTTGG 2 cut(s) 2399, 2431
AloI GAACNNNNNNTCC 2 cut(s) 264, 296
Alw21I GWGCWC 2 cut(s) 11, 655
Alw26I GTCTC 4 cut(s) 1378, 2705, 3773, 5695
AlwI GGATC 8 cut(s) 251, 264, 1343, 1356, 2051, 4585, 4840, 5153
AlwNI CAGNNNCTG 1 cut(s) 1730
Ama87I CYCGRG 2 cut(s) 10, 1818
Aor13HI TCCGGA 2 cut(s) 2829, 4198
Aor51HI AGCGCT 1 cut(s) 645
ArsI GACNNNNNNTTYG 2 cut(s) 264, 296
Asp700I GAANNNNTTC 5 cut(s) 990, 1191, 1802, 3276, 4093
Asp718I GGTACC 1 cut(s) 496
AspA2I CCTAGG 1 cut(s) 203
AspLEI GCGC 6 cut(s) 247, 646, 1027, 1944, 3174, 5659
AspS9I GGNCC 8 cut(s) 18, 41, 1547, 2331, 2574, 3379, 3875, 4678
AsuHPI GGTGA 5 cut(s) 1058, 3680, 3905, 4745, 5142
AsuII TTCGAA 2 cut(s) 1459, 2437
AsuNHI GCTAGC 2 cut(s) 2591, 2887
AvaI CYCGRG 2 cut(s) 10, 1818
AvaII GGWCC 6 cut(s) 18, 1547, 2331, 3379, 3875, 4678
AvrII CCTAGG 1 cut(s) 203
BaeGI GKGCMC 4 cut(s) 141, 3150, 3616, 5545
BaeI ACNNNNGTAYC 2 cut(s) 480, 513
BalI TGGCCA 3 cut(s) 446, 2985, 4194
BamHI GGATCC 2 cut(s) 256, 1348
BanI GGYRCC 2 cut(s) 496, 5396
BanII GRGCYC 3 cut(s) 11, 1972, 2209
BarI GAAGNNNNNNTAC 2 cut(s) 5337, 5369
BauI CACGAG 2 cut(s) 940, 3936
BbsI GAAGAC 5 cut(s) 546, 1579, 2005, 2505, 5644
Bbv12I GWGCWC 2 cut(s) 11, 655
BceAI ACGGC 2 cut(s) 4413, 4839
BciT130I CCWGG 6 cut(s) 448, 3377, 3527, 3873, 4595, 5038
BciVI GTATCC 2 cut(s) 3533, 3750
BclI TGATCA 1 cut(s) 2505
BcoDI GTCTC 4 cut(s) 1378, 2705, 3773, 5695
BcuI ACTAGT 1 cut(s) 4130
BfoI RGCGCY 1 cut(s) 647
BfrI CTTAAG 3 cut(s) 4267, 5198, 5579
BfuAI ACCTGC 2 cut(s) 508, 3348
BfuI GTATCC 2 cut(s) 3533, 3750
BglII AGATCT 2 cut(s) 2524, 5500
BlnI CCTAGG 1 cut(s) 203
BlpI GCTNAGC 1 cut(s) 1971
BmcAI AGTACT 1 cut(s) 3407
Bme1390I CCNGG 6 cut(s) 448, 3377, 3527, 3873, 4595, 5038
Bme18I GGWCC 6 cut(s) 18, 1547, 2331, 3379, 3875, 4678
BmeT110I CYCGRG 2 cut(s) 10, 1818
BmgT120I GGNCC 8 cut(s) 18, 41, 1547, 2331, 2574, 3379, 3875, 4678
BmrFI CCNGG 6 cut(s) 448, 3377, 3527, 3873, 4595, 5038
BmrI ACTGGG 1 cut(s) 4673
BmsI GCATC 9 cut(s) 106, 1480, 1567, 1614, 2224, 2386, 3151, 3870, 4259
BmtI GCTAGC 2 cut(s) 2595, 2891
BmuI ACTGGG 1 cut(s) 4673
BpiI GAAGAC 5 cut(s) 546, 1579, 2005, 2505, 5644
BpmI CTGGAG 1 cut(s) 4727
Bpu10I CCTNAGC 1 cut(s) 485
Bpu1102I GCTNAGC 1 cut(s) 1971
Bpu14I TTCGAA 2 cut(s) 1459, 2437
BpuEI CTTGAG 4 cut(s) 890, 3707, 4891, 5249
BsaBI GATNNNNATC 1 cut(s) 2523
BsaHI GRCGYC 3 cut(s) 543, 4450, 4555
BsaJI CCNNGG 9 cut(s) 21, 203, 229, 4620, 4686, 4728, 4855, 5215, 5443
BsaWI WCCGGW 3 cut(s) 433, 2829, 4198
BsaXI ACNNNNNCTCC 6 cut(s) 3029, 3059, 3224, 3254, 5634, 5664
Bse3DI GCAATG 4 cut(s) 1020, 1141, 2193, 2445
Bse8I GATNNNNATC 1 cut(s) 2523
BseAI TCCGGA 2 cut(s) 2829, 4198
BseBI CCWGG 6 cut(s) 448, 3377, 3527, 3873, 4595, 5038
BseDI CCNNGG 9 cut(s) 21, 203, 229, 4620, 4686, 4728, 4855, 5215, 5443
BseJI GATNNNNATC 1 cut(s) 2523
BseMI GCAATG 4 cut(s) 1020, 1141, 2193, 2445
BseMII CTCAG 9 cut(s) 621, 873, 1187, 1675, 1985, 2474, 2731, 4163, 5706
BseRI GAGGAG 1 cut(s) 3791
BseSI GKGCMC 4 cut(s) 141, 3150, 3616, 5545
BseYI CCCAGC 3 cut(s) 3565, 4603, 5614
BsgI GTGCAG 2 cut(s) 290, 941
Bsh1236I CGCG 1 cut(s) 5657
BshNI GGYRCC 2 cut(s) 496, 5396
BsiHKAI GWGCWC 2 cut(s) 11, 655
BsiHKCI CYCGRG 2 cut(s) 10, 1818
BsiSI CCGG 4 cut(s) 434, 2830, 4199, 5763
BslFI GGGAC 3 cut(s) 31, 3471, 3848
BsmAI GTCTC 4 cut(s) 1378, 2705, 3773, 5695
BsmFI GGGAC 3 cut(s) 31, 3471, 3848
BsoBI CYCGRG 2 cut(s) 10, 1818
Bsp119I TTCGAA 2 cut(s) 1459, 2437
Bsp1286I GDGCHC 8 cut(s) 11, 141, 655, 1972, 2209, 3150, 3616, 5545
Bsp13I TCCGGA 2 cut(s) 2829, 4198
Bsp1407I TGTACA 1 cut(s) 901
Bsp1720I GCTNAGC 1 cut(s) 1971
Bsp19I CCATGG 2 cut(s) 229, 4686
BspCNI CTCAG 9 cut(s) 622, 874, 1188, 1674, 1984, 2473, 2730, 4164, 5705
BspEI TCCGGA 2 cut(s) 2829, 4198
BspFNI CGCG 1 cut(s) 5657
BspHI TCATGA 4 cut(s) 1611, 3201, 4327, 5460
BspMAI CTGCAG 2 cut(s) 3345, 4758
BspMI ACCTGC 2 cut(s) 508, 3348
BspOI GCTAGC 2 cut(s) 2595, 2891
BspPI GGATC 8 cut(s) 251, 264, 1343, 1356, 2051, 4585, 4840, 5153
BspQI GCTCTTC 4 cut(s) 1673, 5178, 5614, 5720
BspT104I TTCGAA 2 cut(s) 1459, 2437
BspT107I GGYRCC 2 cut(s) 496, 5396
BspTI CTTAAG 3 cut(s) 4267, 5198, 5579
BsrBI CCGCTC 2 cut(s) 15, 939
BsrDI GCAATG 4 cut(s) 1020, 1141, 2193, 2445
BsrGI TGTACA 1 cut(s) 901
BssECI CCNNGG 9 cut(s) 21, 203, 229, 4620, 4686, 4728, 4855, 5215, 5443
BssNAI GTATAC 2 cut(s) 2224, 2801
BssNI GRCGYC 3 cut(s) 543, 4450, 4555
BssSI CACGAG 2 cut(s) 940, 3936
BssT1I CCWWGG 7 cut(s) 21, 203, 229, 4620, 4686, 4728, 5443
Bst1107I GTATAC 2 cut(s) 2224, 2801
Bst2BI CACGAG 2 cut(s) 940, 3936
Bst2UI CCWGG 6 cut(s) 448, 3377, 3527, 3873, 4595, 5038
Bst6I CTCTTC 8 cut(s) 432, 1673, 1987, 4223, 5108, 5178, 5614, 5720
BstACI GRCGYC 3 cut(s) 543, 4450, 4555
BstAFI CTTAAG 3 cut(s) 4267, 5198, 5579
BstAPI GCANNNNNTGC 1 cut(s) 4883
BstAUI TGTACA 1 cut(s) 901
BstBI TTCGAA 2 cut(s) 1459, 2437
BstDSI CCRYGG 4 cut(s) 229, 4686, 4855, 5215
BstFNI CGCG 1 cut(s) 5657
BstH2I RGCGCY 1 cut(s) 647
BstHHI GCGC 6 cut(s) 247, 646, 1027, 1944, 3174, 5659
BstMAI GTCTC 4 cut(s) 1378, 2705, 3773, 5695
BstNI CCWGG 6 cut(s) 448, 3377, 3527, 3873, 4595, 5038
BstNSI RCATGY 2 cut(s) 3211, 3238
BstSCI CCNGG 6 cut(s) 446, 3375, 3525, 3871, 4593, 5036
BstSLI GKGCMC 4 cut(s) 141, 3150, 3616, 5545
BstUI CGCG 1 cut(s) 5657
BstV2I GAAGAC 5 cut(s) 546, 1579, 2005, 2505, 5644
BstX2I RGATCY 7 cut(s) 256, 1348, 2524, 4590, 4832, 5145, 5500
BstXI CCANNNNNNTGG 2 cut(s) 28, 4693
BstYI RGATCY 7 cut(s) 256, 1348, 2524, 4590, 4832, 5145, 5500
BstZ17I GTATAC 2 cut(s) 2224, 2801
BsuI GTATCC 2 cut(s) 3533, 3750
BtgI CCRYGG 4 cut(s) 229, 4686, 4855, 5215
BtsI GCAGTG 3 cut(s) 2018, 2700, 4722
BveI ACCTGC 2 cut(s) 508, 3348
CaiI CAGNNNCTG 1 cut(s) 1730
CciI TCATGA 4 cut(s) 1611, 3201, 4327, 5460
CfoI GCGC 6 cut(s) 247, 646, 1027, 1944, 3174, 5659
Cfr13I GGNCC 8 cut(s) 18, 41, 1547, 2331, 2574, 3379, 3875, 4678
CseI GACGC 2 cut(s) 2909, 4439
DraI TTTAAA 2 cut(s) 697, 1128
DraIII CACNNNGTG 3 cut(s) 2419, 4921, 5538
DrdI GACNNNNNNGTC 2 cut(s) 76, 864
DseDI GACNNNNNNGTC 2 cut(s) 76, 864
EaeI YGGCCR 5 cut(s) 444, 1340, 2983, 4192, 4852
Eam1104I CTCTTC 8 cut(s) 432, 1673, 1987, 4223, 5108, 5178, 5614, 5720
EarI CTCTTC 8 cut(s) 432, 1673, 1987, 4223, 5108, 5178, 5614, 5720
Ecl136II GAGCTC 1 cut(s) 9
Eco130I CCWWGG 7 cut(s) 21, 203, 229, 4620, 4686, 4728, 5443
Eco147I AGGCCT 1 cut(s) 5739
Eco24I GRGCYC 3 cut(s) 11, 1972, 2209
Eco32I GATATC 2 cut(s) 2091, 2947
Eco47I GGWCC 6 cut(s) 18, 1547, 2331, 3379, 3875, 4678
Eco47III AGCGCT 1 cut(s) 645
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 6 cut(s) 558, 1005, 1789, 2275, 2337, 2913
Eco88I CYCGRG 2 cut(s) 10, 1818
EcoICRI GAGCTC 1 cut(s) 9
EcoO109I RGGNCCY 2 cut(s) 1547, 3875
EcoRI GAATTC 4 cut(s) 389, 4019, 5223, 5421
EcoRII CCWGG 6 cut(s) 446, 3375, 3525, 3871, 4593, 5036
EcoRV GATATC 2 cut(s) 2091, 2947
EcoT14I CCWWGG 7 cut(s) 21, 203, 229, 4620, 4686, 4728, 5443
EcoT22I ATGCAT 3 cut(s) 693, 1675, 2004
EcoT38I GRGCYC 3 cut(s) 11, 1972, 2209
ErhI CCWWGG 7 cut(s) 21, 203, 229, 4620, 4686, 4728, 5443
FalI AAGNNNNNCTT 4 cut(s) 2399, 2431, 5421, 5453
FaqI GGGAC 3 cut(s) 31, 3471, 3848
FauI CCCGC 1 cut(s) 8
FauNDI CATATG 2 cut(s) 1040, 5626
FbaI TGATCA 1 cut(s) 2505
FblI GTMKAC 7 cut(s) 80, 722, 2223, 2790, 2800, 4965, 5383
FriOI GRGCYC 3 cut(s) 11, 1972, 2209
GlaI GCGC 6 cut(s) 246, 645, 1026, 1943, 3173, 5658
GsaI CCCAGC 3 cut(s) 3569, 4607, 5618
GsuI CTGGAG 1 cut(s) 4727
HaeII RGCGCY 1 cut(s) 647
HapII CCGG 4 cut(s) 434, 2830, 4199, 5763
HgaI GACGC 2 cut(s) 2909, 4439
HhaI GCGC 6 cut(s) 247, 646, 1027, 1944, 3174, 5659
Hin1I GRCGYC 3 cut(s) 543, 4450, 4555
Hin6I GCGC 6 cut(s) 245, 644, 1025, 1942, 3172, 5657
HinP1I GCGC 6 cut(s) 245, 644, 1025, 1942, 3172, 5657
HincII GTYRAC 2 cut(s) 723, 3735
HindII GTYRAC 2 cut(s) 723, 3735
HindIII AAGCTT 4 cut(s) 1050, 2063, 3866, 3990
HpaI GTTAAC 1 cut(s) 3735
HpaII CCGG 4 cut(s) 434, 2830, 4199, 5763
HphI GGTGA 5 cut(s) 1058, 3680, 3905, 4745, 5142
Hpy99I CGWCG 6 cut(s) 40, 106, 116, 4038, 4455, 5785
HpyCH4IV ACGT 3 cut(s) 543, 3737, 4555
HpySE526I ACGT 3 cut(s) 543, 3737, 4555
Hsp92I GRCGYC 3 cut(s) 543, 4450, 4555
HspAI GCGC 6 cut(s) 245, 644, 1025, 1942, 3172, 5657
Kpn2I TCCGGA 2 cut(s) 2829, 4198
KpnI GGTACC 1 cut(s) 500
Ksp22I TGATCA 1 cut(s) 2505
KspAI GTTAAC 1 cut(s) 3735
LguI GCTCTTC 4 cut(s) 1673, 5178, 5614, 5720
LweI GCATC 9 cut(s) 106, 1480, 1567, 1614, 2224, 2386, 3151, 3870, 4259
MaeII ACGT 3 cut(s) 543, 3737, 4555
MbiI CCGCTC 2 cut(s) 15, 939
MfeI CAATTG 2 cut(s) 1004, 2211
MflI RGATCY 7 cut(s) 256, 1348, 2524, 4590, 4832, 5145, 5500
MhlI GDGCHC 8 cut(s) 11, 141, 655, 1972, 2209, 3150, 3616, 5545
MlsI TGGCCA 3 cut(s) 446, 2985, 4194
MluNI TGGCCA 3 cut(s) 446, 2985, 4194
MlyI GAGTC 8 cut(s) 26, 289, 306, 1810, 2351, 3250, 4209, 4971
Mox20I TGGCCA 3 cut(s) 446, 2985, 4194
Mph1103I ATGCAT 3 cut(s) 693, 1675, 2004
MroI TCCGGA 2 cut(s) 2829, 4198
MroXI GAANNNNTTC 5 cut(s) 990, 1191, 1802, 3276, 4093
MscI TGGCCA 3 cut(s) 446, 2985, 4194
Msp20I TGGCCA 3 cut(s) 446, 2985, 4194
MspA1I CMGCKG 3 cut(s) 4045, 4753, 4759
MspCI CTTAAG 3 cut(s) 4267, 5198, 5579
MspI CCGG 4 cut(s) 434, 2830, 4199, 5763
MspR9I CCNGG 6 cut(s) 448, 3377, 3527, 3873, 4595, 5038
MunI CAATTG 2 cut(s) 1004, 2211
MvaI CCWGG 6 cut(s) 448, 3377, 3527, 3873, 4595, 5038
MvnI CGCG 1 cut(s) 5657
NcoI CCATGG 2 cut(s) 229, 4686
NdeI CATATG 2 cut(s) 1040, 5626
NheI GCTAGC 2 cut(s) 2591, 2887
NmeAIII GCCGAG 1 cut(s) 2669
NmuCI GTSAC 6 cut(s) 1561, 1948, 3230, 3695, 4138, 4913
NsiI ATGCAT 3 cut(s) 693, 1675, 2004
NspI RCATGY 2 cut(s) 3211, 3238
NspV TTCGAA 2 cut(s) 1459, 2437
PaeR7I CTCGAG 1 cut(s) 10
PagI TCATGA 4 cut(s) 1611, 3201, 4327, 5460
PceI AGGCCT 1 cut(s) 5739
PciSI GCTCTTC 4 cut(s) 1673, 5178, 5614, 5720
PcsI WCGNNNNNNNCGW 3 cut(s) 280, 321, 1825
PdmI GAANNNNTTC 5 cut(s) 990, 1191, 1802, 3276, 4093
PflMI CCANNNNNTGG 3 cut(s) 1102, 3157, 5538
PfoI TCCNGGA 3 cut(s) 3375, 3525, 4593
PleI GAGTC 8 cut(s) 26, 289, 306, 1810, 2350, 3250, 4209, 4970
PpsI GAGTC 8 cut(s) 26, 289, 306, 1810, 2350, 3250, 4209, 4970
PpuMI RGGWCCY 2 cut(s) 1547, 3875
PsiI TTATAA 4 cut(s) 1845, 2480, 2960, 4319
Psp124BI GAGCTC 1 cut(s) 11
Psp5II RGGWCCY 2 cut(s) 1547, 3875
Psp6I CCWGG 6 cut(s) 446, 3375, 3525, 3871, 4593, 5036
PspFI CCCAGC 3 cut(s) 3565, 4603, 5614
PspGI CCWGG 6 cut(s) 446, 3375, 3525, 3871, 4593, 5036
PspPI GGNCC 8 cut(s) 18, 41, 1547, 2331, 2574, 3379, 3875, 4678
PspPPI RGGWCCY 2 cut(s) 1547, 3875
PspXI VCTCGAGB 1 cut(s) 10
PstI CTGCAG 2 cut(s) 3345, 4758
PstNI CAGNNNCTG 1 cut(s) 1730
PsuI RGATCY 7 cut(s) 256, 1348, 2524, 4590, 4832, 5145, 5500
PvuII CAGCTG 2 cut(s) 4753, 4759
SacI GAGCTC 1 cut(s) 11
SalI GTCGAC 1 cut(s) 721
SapI GCTCTTC 4 cut(s) 1673, 5178, 5614, 5720
Sau96I GGNCC 8 cut(s) 18, 41, 1547, 2331, 2574, 3379, 3875, 4678
ScaI AGTACT 1 cut(s) 3407
SchI GAGTC 8 cut(s) 26, 289, 306, 1810, 2351, 3250, 4209, 4971
ScrFI CCNGG 6 cut(s) 448, 3377, 3527, 3873, 4595, 5038
SduI GDGCHC 8 cut(s) 11, 141, 655, 1972, 2209, 3150, 3616, 5545
SfaNI GCATC 9 cut(s) 106, 1480, 1567, 1614, 2224, 2386, 3151, 3870, 4259
Sfr274I CTCGAG 1 cut(s) 10
SfuI TTCGAA 2 cut(s) 1459, 2437
SinI GGWCC 6 cut(s) 18, 1547, 2331, 3379, 3875, 4678
SlaI CTCGAG 1 cut(s) 10
SmlI CTYRAG 8 cut(s) 10, 869, 3722, 4267, 4906, 5198, 5228, 5579
SmoI CTYRAG 8 cut(s) 10, 869, 3722, 4267, 4906, 5198, 5228, 5579
SpeI ACTAGT 1 cut(s) 4130
SseBI AGGCCT 1 cut(s) 5739
SspI AATATT 4 cut(s) 1572, 2182, 2256, 3718
SstI GAGCTC 1 cut(s) 11
StuI AGGCCT 1 cut(s) 5739
StyD4I CCNGG 6 cut(s) 446, 3375, 3525, 3871, 4593, 5036
StyI CCWWGG 7 cut(s) 21, 203, 229, 4620, 4686, 4728, 5443
TaiI ACGT 3 cut(s) 546, 3740, 4558
TaqII GACCGA 1 cut(s) 5446
TatI WGTACW 8 cut(s) 378, 469, 901, 2685, 3405, 3492, 4902, 5467
TauI GCSGC 4 cut(s) 46, 225, 1583, 2098
TseFI GTSAC 6 cut(s) 1561, 1948, 3230, 3695, 4138, 4913
Tsp45I GTSAC 6 cut(s) 1561, 1948, 3230, 3695, 4138, 4913
TspGWI ACGGA 8 cut(s) 119, 576, 638, 1930, 2298, 4824, 5055, 5204
Van91I CCANNNNNTGG 3 cut(s) 1102, 3157, 5538
Vha464I CTTAAG 3 cut(s) 4267, 5198, 5579
VpaK11BI GGWCC 6 cut(s) 18, 1547, 2331, 3379, 3875, 4678
XbaI TCTAGA 2 cut(s) 3470, 3508
XceI RCATGY 2 cut(s) 3211, 3238
XcmI CCANNNNNNNNNTGG 1 cut(s) 5023
XhoI CTCGAG 1 cut(s) 10
XmaJI CCTAGG 1 cut(s) 203
XmiI GTMKAC 7 cut(s) 80, 722, 2223, 2790, 2800, 4965, 5383
XmnI GAANNNNTTC 5 cut(s) 990, 1191, 1802, 3276, 4093
ZraI GACGTC 2 cut(s) 544, 4556
ZrmI AGTACT 1 cut(s) 3407
Zsp2I ATGCAT 3 cut(s) 693, 1675, 2004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.