RLG00000020575

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009173
Physical Location & Seq
Reverse (-)
66392472 .. 66408098
15627 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020575

Sequence Viewer

Length: 5820 bp
ATGGCGAGCTCCAGCGGGACCAAGGCCGATGCGCGGCCGCAGAGGTCGCTGTCGAGGGGAATGACGAGAATGGCTACTAGAGTTGTGGACTTTCCGACGGAGGGCGACGATGTGTGGGACAGTGGAGTTGTGCCGTCGTGCCTTGCGTCTATTGCTCCGATTTTCAGGGTTGCCAATGAGATTGAGAAGGACAACCCTAGGGTTGCTTATCTCTGCCGCTTCCATGGATTTGAAAAGGCGCATACCATGGATCCAACATCAAGTGGACGTGGAGTTCGTCAGTTTAAGACGCATCTTTTGCACAGACTCGAAAGGGACGAAGTAGATACAAAACATCAGCTTGCTAAATCTGATACAAAAGAAATCATGTTGTATTACCACCAATTCTATGAGAAAAATATCAGAGATGGAGAATATACAAAGAAACCGGAAGAGATGGCCAGGATTTGTCAGATTGCAACTGTACTGTATGATGTGCTCAGGACAGTGGTACCTGCCTCTCAAATAGACATACAGACCCAGAGAATTGCTGAAGATGTCAAGAGGAAAAGGGAGCAATATGTAAACTATAACATTCTTCCATTATATACTGTCGGGGTTAAACCGACAATTATGGAACTTCCTGAGATCAAAGCAGCGCTCCGTGCTCTACAGAATGTGAGTAATCTTCCAATGCCAAGAATGCATTTTAAACCCACGAATCCTGATGACAAGTCGACAGTGCCTACAGAGAGGATTAAACCTGTGAATGACATACTTGATTGGCTTTCGGCTATTTTTGGGTTTCAGAAAGGAAATGTGGCAAATCAGAGGGAACATTTAATTTTGCTACTTGCCAACATTGATGTAAGACATAGAAGTCCTGAGAACTACAATGAGTTGAATAGTGACACTGTACAGCATTTGATGGAGAAATTCTTTAAGAATTATCGTTCATGGAATAACTATCTGCATTGTAAAACAAATCTTCGGTTCCCCGAAGGTTCTCACAGCAAACAACAATTGGAACTTATATACATTGCTCTCTATCTTCTCATATGGGGTGAAGCTTCAAATGTCCGATTCATGCCTGAATGCTTATGCTACATTTTCCATAATATGGCGAACGAGGTCTATGGAATTTTAAATAGCAATGCTCATACTGTTAGTGGAGAAACATACCAAACAGTAGCACGTGATGAAGAATCTTTTTTGAGGGATGTTATAGCTCCTATCTACCAGGTTTTGTACAAGGAAGCAAAGAGAAACAAAATGGGCAAGGCAAGTCATTCAAGATGGAGAAATTATGATGATTTGAATGAATACTTCTGGTCGGACAAATGTTTTAAGCTAGGGTGGCCAATGGATCCTAAAGCAGATTTCTTTAGGCATTCAGATGGGATACAACCAGTAAATGAGAGGACCAATCAAGCTACTGGTGGTAGGAGGAAGCCTAAAACAAATTTTGTAGAAGTCCGAACATTTTTACACCTCTACAGAAGTTTTGATCGCATGTGGATATTCTTCATACTGGCTTTACAGGCTATGATTATTATTGCATGGAGTCCTTCTGGATCTATCACTTCAATTTTTGATGCGGACGTCTTCAGAAGTGTTTTAAGCATCTTCATGACACATGCTTTCCTTAATTTATTACAAGCCACTTTGGACATAATTCTCAGTTGGAATGCATGGAAGAGCTTGAAATTCACTCAAATATTACGATACCTCTTGAAATTTGCAGTAGCTGTTGTCTGGGCTGTGGTTCTGCCTATTGGTTATTCTAGTTCTATGCAGAATCCTACAGGGCTTCTGAAATTCTTCAGTAGTTGGAGTCGGGATTGGCGGAATCAGTCATTTTATAATTATGCTGTTGCAATCTATATGGTTCCTAATTTATTGGCTGCTATGCTATTTTTTCTTCCACCTTTACGAAGACACATAGAGCGTTCCAACTGGCGCATTGTCACTCTTTTGATGTGGTGGGCTCAGCCAAAACTGTATGTCGGAAGAGGAATGCATGAGGATGTCTTCTCACTGCTGAAATATACATTGTTCTGGATCATGCTGCTAATCAGCAAGCTAGCGTTCAGCTATTATGTGGAGATATTGCCGCTTGTTGCACCAACAAAGTTGATAATGGATATGCCTATCAGTAATTACCAATGGCATGAGTTCTTTCCGAATGCAACTCATAATATTGGTGTTGTCATTGCAATATGGACTCCAATTATCCTGGTATATTTTATGGATGCACAAATATGGTATGCAATATTTTCTACTCTGTTCGGTGGGATTCATGGAGCCTTCAGCCATTTGGGGGAGATACGAACACTTGGGATGTTGCGGTCCAGATTTGAGTCTGTGCCTTCAGCTTTCAGTGATCGGCTTATGCCATCGTCAGAGAATAAGGATGCCAGAAAGAAGAAACACTTGGTGGTACTCTTTCATCAGTGTTTTCATACTGATGAAGGACTTGTTCGAAAGAACATTGCTAATTTCTCTCAGGTCTGGAATGAATTTATAAATTCTATGCGAATGGAAGACCTGATCAGCAATAGGGATAGGGATCTGCTGCTTGTTCCGTATTCTTCAAGTGATGTTTCTGTTGTCCAGTGGCCTCCTTTCTTGCTTGCTAGCAAGATTCCGATAGCATTAGACATGGCAAAGGATTTTCCAGGGAAGTCAGATGATGATTTATTTAGGAAGATTAGGAATGATGATTATATGTACTCGGCAGTGATTGAATGCTATGAGACACTCAGGGATATTATATTTGGCCTTCTTGAAGATGAAGCTGATAAGATGATTGTAAGGCAGATATGTTATGAAGTCGACAGTAGTATACAGCAGAAGAGGTTTTTGATTAATTTCCGTATGAGTGGGCTGCCATTTCTTAGTGAGAGGTTGGAGAAGTTTCTCAAACTCTTGCTAGCCGAAGATGAGAATGTCGAAAATTCCTTGCGTCAGATAATCAATGTCCTTCAAGATATCATGGAGATTATAACACAGGATGTAATGATCCATGGCCATGAAATCCTCGAAGCAGCTCACTACATAGAGGGTCAAAATGTCAAGAAGGAGCAAAGGTTTCAAAAAATCAACATATATCTTGCCCAAAATCTATCTTGGAGAGAGAAGGTCGTTAGGCTTCATTTGCTTTTGACCGTCAAGGAATCTGCCATCAATGTGCCCCAAAACTTGGATGCTCGCAGGCGCATTACTTTCTTTGCAAACTCCTTATTCATGAACATGCCAAATGCTCCCAAAGTTCGTGACATGCTCTCCTTTAGTGTTCTGACTCCTTATTACAAAGAAGATGTTCTTTATTCTGATGAGGAACTTACCAAGGAAAATGAGGATGGAATCTCAATTTTGTTCTACCTGCAGAAGATATATCCTGATGAATGGACAAATTTCCTGGACCGCATAAATGAACATAACAGTCAGTACACTGAAAAGGACAAGTCGGAGTTAATTCGTCAGTGGGTATCTTACAGGGGACAGACACTTTCTAGAACAGTGAGAGGGATGATGTATTATAGGAAGGCTCTAGACCTTCAATGTATCCTGGAAACAGCAGGAGATGATGGTGTTTATGGAGGCGACGCATCCCAGATTTTAAGTGAAAAAGATGAGAAGGATATTCTTGATCGGGCACAGGCTCTGGCAGATTTGAAGTTCACTTATGTAGTTTCTTGTCAGGTCTATGGTGAACAGAAGAATTCCACTGAAGCCCGAGACAAAAGTTGTTACACTAATATTCTCAAGCTGATGTTAACGTATCATTCTCTACGTGTTGCTTACATAGATACAAGAGAGGAGACAGTGAATGGAAAATCCCAAAAGATTCATTTTTCTGTTCTGGTCAAGGGAGGTGACAAATGGGATGAGGAAATATACCGCATCAAGCTTCCAGGTCCTCCAACAGTAATTGGTGAAGGAAAACCTGAAAATCAAAACCATGCCATCGTATTTACTCGTGGAGAAGCCCTGCAAACCATTGACATGAATCAGGATAATTATTTTGAGGAAGCTTTCAAAATGAGAAATGTATTGGAGGAATTTCTAAAGCCTCGACGGGGGCAGCGGAAACCTACAATATTGGGTCTAAGGGAACATATATTTACAGGAAGTGTTTCATCACTCGCTTGGTTCATGTCCAATCAAGAGACAAGTTTTGTGACTATAGGCCAACGAATTTTGGCAAATCCGCTGAGGGTACGGTTTCATTATGGCCATCCTGATATTTTTGACAGAATCTTTCACATAACAAGGGGCGGCATAAGCAAAGCATCAAAAGTAATTAACTTAAGCGAGGATATATTTTCAGGATATAATTCTGTTCTGCGTGGGGGATTTATAACACATCATGAGTATATCCAAGTAGGCAAGGGGCGTGATGTTGGAATGAATCAAATATCGCTTTTCGAGGCTAAAGTTGCAAATGGAAATGGAGAGCAGACACTTAGCCGTGATGTTTATAGGCTTGGGCGTCGGTTTGATTTCTATAGAATGCTGTCTTTTTACTTCACAACAGTTGGGTTCTATTTTAGTAGTATGGTAACTGTGCTGACTGTCTATGTGTTTTTATATGGACGTCTATATATGGTTATGAGTGGATTGGAAAGTGAGATCCTGGATAGCCCAGCCATACATCAGACCAAGGCTTTTGAACAAGCTCTGGCTACACAGTCTGTCTTTCAACTGGGCTTGTTGCTGGTCCTTCCCATGGTTATGGAAATTGGTCTGGAGAAAGGATTTCGCACTGCCTTGGGTGATTTCATCATCATGCAGCTGCAGCTGGCCTCTGTTTTCTTCACTTTCCAACTAGGAACAAAAGCACATTACTACGGAAGGACAATCTTACATGGAGGATCTAAATATCGAGCAACTGGCCGTGGTTTTGTTGTTTTTCATGCAAAGTTTGCCGATAACTACAGGCTGTACTCACGAAGTCACTTTGTGAAAGGGTTGGAGCTGTTGATTCTTCTGATTGTGTATGGAGTCTATGGGAATGCATATAGGAGTTCAAATCTGTATTTTTTCATCACGTTCTCCATGTGGTTTCTGGTTGCTTCCTGGTTGTTTGCTCCTTTTATCTTTAATCCGTCCAGCTTTGACTGGCAAAAAACTGTAGATGATTGGACGGATTGGAAGAGGTGGATGGGAAATCGTGGTGGTATTGGGATCTCACCTGACAAAAGTTGGGAATCATGGTGGGATGAAGAACAGGAACACCTCAAGCACACTATTATCCGTGGAAGAATTATTGAGATAATTCTTGCATGTCGCTTCTTTTTTTACCAATATGGGATTGTCTACCACCTCGATATAGCTCATTTCAGCAAGAGTTTACTGGTTTATGGACTTTCTTGGGTGGTTATGGTAACGGTGCTTCTCGTCTTAAAGATGGTGTCAATGGGTAGACGAAGATTTGGCACTGACTTTCAGCTCATGTTCAGAATTCTGAAGGCGCTTCTGTTTCTTGGCTTCATGTCAGTCATGACTGTCCTATTTGTAGTTTGTGGCCTTACTATTTCAGATCTATTTGCTGCTATTCTTGCCTTCTTGCCCACAGGGTGGGCACTACTTCTTATCGGGCAAGTATGCAGGCCAATGGTTAAGGGAGTAGGATTCTGGGAATCAATTAAGGAGCTGGGAAGAGCCTATGATTACATAATGGGAGTGGTAGTCTTCATGCCGGTTGCCATTTTGTCATGGTTCCCATTTGTCTCAGAGTTCCAAACACGGTTGCTCTTCAACCAAGCATTCAGTCGAGGCCTACAAATTTCAATGATTCTTGCCGGGAGGAAAGATAAGACAGCTTCTAATAGATAG

Protein Analysis

1940

Amino Acids

225.06

Weight (kDa)

8.88

Isoelectric Point (pI)

40.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 50 - 190 2.6e-10 Vta1 like
FKS1_dom1 PF14288 336 - 447 4.4e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 867 - 1050 4.9e-69 Callose synthase 1 domain
Glucan_synthase PF02364 1057 - 1148 1.3e-29 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1149 - 1751 1.3e-200 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1842, 2504, 2984, 4343
AasI GACNNNNNNGTC 1 cut(s) 858
AatII GACGTC 2 cut(s) 1584, 4582
Acc36I ACCTGC 2 cut(s) 502, 3372
Acc65I GGTACC 1 cut(s) 490
AccB1I GGYRCC 1 cut(s) 490
AccB7I CCANNNNNTGG 3 cut(s) 1099, 3181, 5562
AccI GTMKAC 5 cut(s) 716, 2814, 2824, 5301, 5407
AccII CGCG 1 cut(s) 34
AcoI YGGCCR 6 cut(s) 35, 438, 1337, 3007, 4216, 4876
AcuI CTGAAG 7 cut(s) 552, 1570, 1786, 2272, 2334, 3732, 5471
AcvI CACGTG 1 cut(s) 1175
AcyI GRCGYC 3 cut(s) 1581, 4474, 4579
AdeI CACNNNGTG 3 cut(s) 2416, 4945, 5562
AfaI GTAC 9 cut(s) 465, 492, 897, 1229, 2421, 2711, 3431, 4203, 4928
AfeI AGCGCT 1 cut(s) 639
AfiI CCNNNNNNNGG 8 cut(s) 33, 101, 1099, 2299, 3181, 4061, 4711, 5562
AflII CTTAAG 1 cut(s) 4291
AflIII ACRYGT 1 cut(s) 3775
AjiI CACGTC 1 cut(s) 269
AjnI CCWGG 9 cut(s) 440, 1218, 2214, 2656, 3399, 3549, 3895, 4617, 5060
AjuI GAANNNNNNNTTGG 2 cut(s) 2396, 2428
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
Alw21I GWGCWC 3 cut(s) 11, 480, 649
Alw26I GTCTC 5 cut(s) 2729, 3714, 3797, 4145, 5719
AlwNI CAGNNNCTG 2 cut(s) 1727, 3646
Ama87I CYCGRG 1 cut(s) 3717
Aor51HI AGCGCT 1 cut(s) 639
ArsI GACNNNNNNTTYG 4 cut(s) 258, 280, 290, 312
AseI ATTAAT 1 cut(s) 2847
Asp700I GAANNNNTTC 3 cut(s) 1799, 3300, 4117
Asp718I GGTACC 1 cut(s) 490
AspA2I CCTAGG 1 cut(s) 197
AspLEI GCGC 6 cut(s) 34, 241, 640, 1941, 3198, 5458
AspS9I GGNCC 6 cut(s) 18, 1401, 2328, 3403, 3899, 4702
AsuC2I CCSGG 1 cut(s) 5788
AsuHPI GGTGA 6 cut(s) 1055, 3704, 3869, 3929, 4769, 5166
AsuII TTCGAA 1 cut(s) 2461
AsuNHI GCTAGC 3 cut(s) 2062, 2615, 2911
AvaI CYCGRG 1 cut(s) 3717
AvaII GGWCC 6 cut(s) 18, 1401, 2328, 3403, 3899, 4702
AvrII CCTAGG 1 cut(s) 197
BaeGI GKGCMC 3 cut(s) 3174, 3640, 5569
BaeI ACNNNNGTAYC 2 cut(s) 474, 507
BalI TGGCCA 4 cut(s) 440, 1339, 3009, 4218
BamHI GGATCC 2 cut(s) 250, 1345
BanI GGYRCC 1 cut(s) 490
BanII GRGCYC 2 cut(s) 11, 1969
BarI GAAGNNNNNNTAC 2 cut(s) 5361, 5393
BauI CACGAG 1 cut(s) 3960
BbrPI CACGTG 1 cut(s) 1175
BbsI GAAGAC 5 cut(s) 1576, 1921, 2002, 2529, 5668
Bbv12I GWGCWC 3 cut(s) 11, 480, 649
BbvCI CCTCAGC 1 cut(s) 4196
BceAI ACGGC 3 cut(s) 118, 4437, 4863
BciT130I CCWGG 9 cut(s) 442, 1220, 2216, 2658, 3401, 3551, 3897, 4619, 5062
BciVI GTATCC 2 cut(s) 1374, 3557
BclI TGATCA 1 cut(s) 2529
BcnI CCSGG 1 cut(s) 5788
BcoDI GTCTC 5 cut(s) 2729, 3714, 3797, 4145, 5719
BfoI RGCGCY 2 cut(s) 641, 5459
BfrI CTTAAG 1 cut(s) 4291
BfuAI ACCTGC 2 cut(s) 502, 3372
BfuI GTATCC 2 cut(s) 1374, 3557
BglII AGATCT 1 cut(s) 5524
BlnI CCTAGG 1 cut(s) 197
BlpI GCTNAGC 1 cut(s) 1968
Bme18I GGWCC 6 cut(s) 18, 1401, 2328, 3403, 3899, 4702
BmeT110I CYCGRG 1 cut(s) 3717
BmgBI CACGTC 1 cut(s) 269
BmgT120I GGNCC 6 cut(s) 18, 1401, 2328, 3403, 3899, 4702
BmiI GGNNCC 8 cut(s) 19, 252, 492, 974, 1347, 1869, 2284, 5705
BmrI ACTGGG 1 cut(s) 4697
BmtI GCTAGC 3 cut(s) 2066, 2619, 2915
BmuI ACTGGG 1 cut(s) 4697
BpiI GAAGAC 5 cut(s) 1576, 1921, 2002, 2529, 5668
BpmI CTGGAG 1 cut(s) 4751
Bpu10I CCTNAGC 2 cut(s) 479, 4196
Bpu1102I GCTNAGC 1 cut(s) 1968
Bpu14I TTCGAA 1 cut(s) 2461
BpuEI CTTGAG 2 cut(s) 3731, 5207
BpuMI CCSGG 1 cut(s) 5788
BsaAI YACGTR 2 cut(s) 1175, 3776
BsaBI GATNNNNATC 1 cut(s) 2547
BsaHI GRCGYC 3 cut(s) 1581, 4474, 4579
BsaWI WCCGGW 1 cut(s) 427
BsaXI ACNNNNNCTCC 6 cut(s) 3053, 3083, 3248, 3278, 5658, 5688
Bsc4I CCNNNNNNNGG 8 cut(s) 33, 101, 1099, 2299, 3181, 4061, 4711, 5562
Bse118I RCCGGY 1 cut(s) 5683
Bse1I ACTGG 9 cut(s) 1388, 1420, 1515, 1940, 2593, 4692, 4879, 5108, 5343
Bse3DI GCAATG 4 cut(s) 1017, 1138, 2190, 2469
Bse8I GATNNNNATC 1 cut(s) 2547
BseBI CCWGG 9 cut(s) 442, 1220, 2216, 2658, 3401, 3551, 3897, 4619, 5062
BseJI GATNNNNATC 1 cut(s) 2547
BseLI CCNNNNNNNGG 8 cut(s) 33, 101, 1099, 2299, 3181, 4061, 4711, 5562
BseMI GCAATG 4 cut(s) 1017, 1138, 2190, 2469
BseMII CTCAG 9 cut(s) 493, 615, 855, 1672, 1982, 2498, 2755, 4187, 5730
BseNI ACTGG 9 cut(s) 1388, 1420, 1515, 1940, 2593, 4692, 4879, 5108, 5343
BseRI GAGGAG 1 cut(s) 3815
BseSI GKGCMC 3 cut(s) 3174, 3640, 5569
BseX3I CGGCCG 1 cut(s) 35
BseYI CCCAGC 2 cut(s) 4627, 5638
Bsh1236I CGCG 1 cut(s) 34
Bsh1285I CGRYCG 1 cut(s) 38
BshNI GGYRCC 1 cut(s) 490
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 3 cut(s) 11, 480, 649
BsiHKCI CYCGRG 1 cut(s) 3717
BsiSI CCGG 3 cut(s) 428, 5684, 5787
BslFI GGGAC 4 cut(s) 31, 131, 329, 3495
BslI CCNNNNNNNGG 8 cut(s) 33, 101, 1099, 2299, 3181, 4061, 4711, 5562
BsmAI GTCTC 5 cut(s) 2729, 3714, 3797, 4145, 5719
BsmFI GGGAC 4 cut(s) 31, 131, 329, 3495
BsoBI CYCGRG 1 cut(s) 3717
Bsp119I TTCGAA 1 cut(s) 2461
Bsp1286I GDGCHC 7 cut(s) 11, 480, 649, 1969, 3174, 3640, 5569
Bsp1407I TGTACA 2 cut(s) 895, 1227
Bsp1720I GCTNAGC 1 cut(s) 1968
Bsp19I CCATGG 4 cut(s) 223, 246, 3004, 4710
BspCNI CTCAG 9 cut(s) 492, 616, 856, 1671, 1981, 2497, 2754, 4188, 5729
BspFNI CGCG 1 cut(s) 34
BspHI TCATGA 4 cut(s) 1608, 3225, 4351, 5484
BspLI GGNNCC 8 cut(s) 19, 252, 492, 974, 1347, 1869, 2284, 5705
BspMAI CTGCAG 2 cut(s) 3369, 4782
BspMI ACCTGC 2 cut(s) 502, 3372
BspOI GCTAGC 3 cut(s) 2066, 2619, 2915
BspQI GCTCTTC 3 cut(s) 1670, 5638, 5744
BspT104I TTCGAA 1 cut(s) 2461
BspT107I GGYRCC 1 cut(s) 490
BspTI CTTAAG 1 cut(s) 4291
BsrDI GCAATG 4 cut(s) 1017, 1138, 2190, 2469
BsrFI RCCGGY 1 cut(s) 5683
BsrGI TGTACA 2 cut(s) 895, 1227
BsrI ACTGG 9 cut(s) 1388, 1420, 1515, 1940, 2593, 4692, 4879, 5108, 5343
BssAI RCCGGY 1 cut(s) 5683
BssNAI GTATAC 1 cut(s) 2825
BssNI GRCGYC 3 cut(s) 1581, 4474, 4579
BssSI CACGAG 1 cut(s) 3960
BssT1I CCWWGG 9 cut(s) 21, 197, 223, 246, 3004, 3327, 4644, 4710, 4752
Bst1107I GTATAC 1 cut(s) 2825
Bst2BI CACGAG 1 cut(s) 3960
Bst2UI CCWGG 9 cut(s) 442, 1220, 2216, 2658, 3401, 3551, 3897, 4619, 5062
Bst6I CTCTTC 7 cut(s) 426, 1670, 1984, 2828, 5132, 5638, 5744
BstACI GRCGYC 3 cut(s) 1581, 4474, 4579
BstAFI CTTAAG 1 cut(s) 4291
BstAPI GCANNNNNTGC 2 cut(s) 298, 4907
BstAUI TGTACA 2 cut(s) 895, 1227
BstBAI YACGTR 2 cut(s) 1175, 3776
BstBI TTCGAA 1 cut(s) 2461
BstDSI CCRYGG 6 cut(s) 223, 246, 3004, 4710, 4879, 5239
BstFNI CGCG 1 cut(s) 34
BstH2I RGCGCY 2 cut(s) 641, 5459
BstHHI GCGC 6 cut(s) 34, 241, 640, 1941, 3198, 5458
BstMAI GTCTC 5 cut(s) 2729, 3714, 3797, 4145, 5719
BstMCI CGRYCG 1 cut(s) 38
BstNI CCWGG 9 cut(s) 442, 1220, 2216, 2658, 3401, 3551, 3897, 4619, 5062
BstNSI RCATGY 5 cut(s) 1495, 1619, 3235, 3262, 5271
BstSLI GKGCMC 3 cut(s) 3174, 3640, 5569
BstUI CGCG 1 cut(s) 34
BstV2I GAAGAC 5 cut(s) 1576, 1921, 2002, 2529, 5668
BstX2I RGATCY 8 cut(s) 250, 1345, 1553, 2548, 4614, 4856, 5169, 5524
BstXI CCANNNNNNTGG 1 cut(s) 4717
BstYI RGATCY 8 cut(s) 250, 1345, 1553, 2548, 4614, 4856, 5169, 5524
BstZ17I GTATAC 1 cut(s) 2825
BstZI CGGCCG 1 cut(s) 35
BsuI GTATCC 2 cut(s) 1374, 3557
BtgI CCRYGG 6 cut(s) 223, 246, 3004, 4710, 4879, 5239
BtrI CACGTC 1 cut(s) 269
BtsI GCAGTG 3 cut(s) 2015, 2724, 4746
BveI ACCTGC 2 cut(s) 502, 3372
CaiI CAGNNNCTG 2 cut(s) 1727, 3646
CciI TCATGA 4 cut(s) 1608, 3225, 4351, 5484
CciNI GCGGCCGC 1 cut(s) 35
CfoI GCGC 6 cut(s) 34, 241, 640, 1941, 3198, 5458
Cfr10I RCCGGY 1 cut(s) 5683
Cfr13I GGNCC 6 cut(s) 18, 1401, 2328, 3403, 3899, 4702
CseI GACGC 5 cut(s) 135, 298, 2933, 3596, 4463
CsiI ACCWGGT 1 cut(s) 1218
Csp6I GTAC 9 cut(s) 464, 491, 896, 1228, 2420, 2710, 3430, 4202, 4927
CviQI GTAC 9 cut(s) 464, 491, 896, 1228, 2420, 2710, 3430, 4202, 4927
DraI TTTAAA 2 cut(s) 691, 1125
DraIII CACNNNGTG 3 cut(s) 2416, 4945, 5562
DrdI GACNNNNNNGTC 1 cut(s) 858
DseDI GACNNNNNNGTC 1 cut(s) 858
EaeI YGGCCR 6 cut(s) 35, 438, 1337, 3007, 4216, 4876
EagI CGGCCG 1 cut(s) 35
Eam1104I CTCTTC 7 cut(s) 426, 1670, 1984, 2828, 5132, 5638, 5744
EarI CTCTTC 7 cut(s) 426, 1670, 1984, 2828, 5132, 5638, 5744
EciI GGCGGA 1 cut(s) 1840
Ecl136II GAGCTC 1 cut(s) 9
EclXI CGGCCG 1 cut(s) 35
Eco130I CCWWGG 9 cut(s) 21, 197, 223, 246, 3004, 3327, 4644, 4710, 4752
Eco147I AGGCCT 1 cut(s) 5763
Eco24I GRGCYC 2 cut(s) 11, 1969
Eco32I GATATC 1 cut(s) 2971
Eco47I GGWCC 6 cut(s) 18, 1401, 2328, 3403, 3899, 4702
Eco47III AGCGCT 1 cut(s) 639
Eco52I CGGCCG 1 cut(s) 35
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 7 cut(s) 552, 1570, 1786, 2272, 2334, 3732, 5471
Eco72I CACGTG 1 cut(s) 1175
Eco88I CYCGRG 1 cut(s) 3717
EcoICRI GAGCTC 1 cut(s) 9
EcoO109I RGGNCCY 1 cut(s) 3899
EcoRI GAATTC 2 cut(s) 3703, 5445
EcoRII CCWGG 9 cut(s) 440, 1218, 2214, 2656, 3399, 3549, 3895, 4617, 5060
EcoRV GATATC 1 cut(s) 2971
EcoT14I CCWWGG 9 cut(s) 21, 197, 223, 246, 3004, 3327, 4644, 4710, 4752
EcoT22I ATGCAT 4 cut(s) 687, 1672, 2001, 5002
EcoT38I GRGCYC 2 cut(s) 11, 1969
ErhI CCWWGG 9 cut(s) 21, 197, 223, 246, 3004, 3327, 4644, 4710, 4752
FalI AAGNNNNNCTT 4 cut(s) 2396, 2428, 3288, 3320
FaqI GGGAC 4 cut(s) 31, 131, 329, 3495
FauI CCCGC 1 cut(s) 8
FauNDI CATATG 1 cut(s) 1037
FbaI TGATCA 1 cut(s) 2529
FblI GTMKAC 5 cut(s) 716, 2814, 2824, 5301, 5407
FriOI GRGCYC 2 cut(s) 11, 1969
GlaI GCGC 6 cut(s) 33, 240, 639, 1940, 3197, 5457
GsaI CCCAGC 2 cut(s) 4631, 5642
GsuI CTGGAG 1 cut(s) 4751
HaeII RGCGCY 2 cut(s) 641, 5459
HapII CCGG 3 cut(s) 428, 5684, 5787
HgaI GACGC 5 cut(s) 135, 298, 2933, 3596, 4463
HhaI GCGC 6 cut(s) 34, 241, 640, 1941, 3198, 5458
Hin1I GRCGYC 3 cut(s) 1581, 4474, 4579
Hin6I GCGC 6 cut(s) 32, 239, 638, 1939, 3196, 5456
HinP1I GCGC 6 cut(s) 32, 239, 638, 1939, 3196, 5456
HincII GTYRAC 3 cut(s) 717, 2815, 3759
HindII GTYRAC 3 cut(s) 717, 2815, 3759
HindIII AAGCTT 3 cut(s) 1047, 3890, 4014
HpaI GTTAAC 1 cut(s) 3759
HpaII CCGG 3 cut(s) 428, 5684, 5787
HphI GGTGA 6 cut(s) 1055, 3704, 3869, 3929, 4769, 5166
Hpy99I CGWCG 6 cut(s) 100, 110, 139, 3590, 4062, 4479
HpyCH4IV ACGT 7 cut(s) 268, 1174, 1581, 3761, 3775, 4579, 5033
HpySE526I ACGT 7 cut(s) 268, 1174, 1581, 3761, 3775, 4579, 5033
Hsp92I GRCGYC 3 cut(s) 1581, 4474, 4579
HspAI GCGC 6 cut(s) 32, 239, 638, 1939, 3196, 5456
KpnI GGTACC 1 cut(s) 494
Ksp22I TGATCA 1 cut(s) 2529
KspAI GTTAAC 1 cut(s) 3759
LguI GCTCTTC 3 cut(s) 1670, 5638, 5744
MabI ACCWGGT 1 cut(s) 1218
MaeII ACGT 7 cut(s) 268, 1174, 1581, 3761, 3775, 4579, 5033
MaeIII GTNAC 9 cut(s) 887, 1945, 3254, 3731, 3857, 4162, 4543, 4937, 5368
MfeI CAATTG 1 cut(s) 1001
MflI RGATCY 8 cut(s) 250, 1345, 1553, 2548, 4614, 4856, 5169, 5524
MhlI GDGCHC 7 cut(s) 11, 480, 649, 1969, 3174, 3640, 5569
MlsI TGGCCA 4 cut(s) 440, 1339, 3009, 4218
MluNI TGGCCA 4 cut(s) 440, 1339, 3009, 4218
MlyI GAGTC 7 cut(s) 300, 1552, 1822, 2197, 2348, 3274, 4995
Mox20I TGGCCA 4 cut(s) 440, 1339, 3009, 4218
Mph1103I ATGCAT 4 cut(s) 687, 1672, 2001, 5002
MroXI GAANNNNTTC 3 cut(s) 1799, 3300, 4117
MscI TGGCCA 4 cut(s) 440, 1339, 3009, 4218
Msp20I TGGCCA 4 cut(s) 440, 1339, 3009, 4218
MspA1I CMGCKG 5 cut(s) 15, 4069, 4195, 4777, 4783
MspCI CTTAAG 1 cut(s) 4291
MspI CCGG 3 cut(s) 428, 5684, 5787
MunI CAATTG 1 cut(s) 1001
MvaI CCWGG 9 cut(s) 442, 1220, 2216, 2658, 3401, 3551, 3897, 4619, 5062
MvnI CGCG 1 cut(s) 34
NciI CCSGG 1 cut(s) 5788
NcoI CCATGG 4 cut(s) 223, 246, 3004, 4710
NdeI CATATG 1 cut(s) 1037
NheI GCTAGC 3 cut(s) 2062, 2615, 2911
NlaIV GGNNCC 8 cut(s) 19, 252, 492, 974, 1347, 1869, 2284, 5705
NmeAIII GCCGAG 1 cut(s) 2693
NmuCI GTSAC 6 cut(s) 887, 1945, 3254, 3857, 4162, 4937
NotI GCGGCCGC 1 cut(s) 35
NsiI ATGCAT 4 cut(s) 687, 1672, 2001, 5002
NspI RCATGY 5 cut(s) 1495, 1619, 3235, 3262, 5271
NspV TTCGAA 1 cut(s) 2461
PagI TCATGA 4 cut(s) 1608, 3225, 4351, 5484
PceI AGGCCT 1 cut(s) 5763
PciSI GCTCTTC 3 cut(s) 1670, 5638, 5744
PcsI WCGNNNNNNNCGW 3 cut(s) 143, 274, 315
PdmI GAANNNNTTC 3 cut(s) 1799, 3300, 4117
PflMI CCANNNNNTGG 3 cut(s) 1099, 3181, 5562
PfoI TCCNGGA 3 cut(s) 3399, 3549, 4617
PleI GAGTC 7 cut(s) 300, 1551, 1821, 2197, 2347, 3274, 4994
PmaCI CACGTG 1 cut(s) 1175
PmlI CACGTG 1 cut(s) 1175
PpsI GAGTC 7 cut(s) 300, 1551, 1821, 2197, 2347, 3274, 4994
Ppu21I YACGTR 2 cut(s) 1175, 3776
PpuMI RGGWCCY 1 cut(s) 3899
PshBI ATTAAT 1 cut(s) 2847
PsiI TTATAA 4 cut(s) 1842, 2504, 2984, 4343
Psp124BI GAGCTC 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 3899
Psp6I CCWGG 9 cut(s) 440, 1218, 2214, 2656, 3399, 3549, 3895, 4617, 5060
PspCI CACGTG 1 cut(s) 1175
PspFI CCCAGC 2 cut(s) 4627, 5638
PspGI CCWGG 9 cut(s) 440, 1218, 2214, 2656, 3399, 3549, 3895, 4617, 5060
PspN4I GGNNCC 8 cut(s) 19, 252, 492, 974, 1347, 1869, 2284, 5705
PspPI GGNCC 6 cut(s) 18, 1401, 2328, 3403, 3899, 4702
PspPPI RGGWCCY 1 cut(s) 3899
PstI CTGCAG 2 cut(s) 3369, 4782
PstNI CAGNNNCTG 2 cut(s) 1727, 3646
PsuI RGATCY 8 cut(s) 250, 1345, 1553, 2548, 4614, 4856, 5169, 5524
PvuII CAGCTG 2 cut(s) 4777, 4783
RsaI GTAC 9 cut(s) 465, 492, 897, 1229, 2421, 2711, 3431, 4203, 4928
RsaNI GTAC 9 cut(s) 464, 491, 896, 1228, 2420, 2710, 3430, 4202, 4927
SacI GAGCTC 1 cut(s) 11
SalI GTCGAC 2 cut(s) 715, 2813
SapI GCTCTTC 3 cut(s) 1670, 5638, 5744
Sau96I GGNCC 6 cut(s) 18, 1401, 2328, 3403, 3899, 4702
SchI GAGTC 7 cut(s) 300, 1552, 1822, 2197, 2348, 3274, 4995
SduI GDGCHC 7 cut(s) 11, 480, 649, 1969, 3174, 3640, 5569
SexAI ACCWGGT 1 cut(s) 1218
SfuI TTCGAA 1 cut(s) 2461
SinI GGWCC 6 cut(s) 18, 1401, 2328, 3403, 3899, 4702
SmlI CTYRAG 3 cut(s) 3746, 4291, 5222
SmoI CTYRAG 3 cut(s) 3746, 4291, 5222
SseBI AGGCCT 1 cut(s) 5763
SspI AATATT 5 cut(s) 1698, 2179, 2253, 3742, 4083
SstI GAGCTC 1 cut(s) 11
StuI AGGCCT 1 cut(s) 5763
StyI CCWWGG 9 cut(s) 21, 197, 223, 246, 3004, 3327, 4644, 4710, 4752
TaiI ACGT 7 cut(s) 271, 1177, 1584, 3764, 3778, 4582, 5036
TatI WGTACW 6 cut(s) 463, 895, 1227, 2709, 3429, 4926
TauI GCSGC 5 cut(s) 37, 40, 219, 2095, 4263
TseFI GTSAC 6 cut(s) 887, 1945, 3254, 3857, 4162, 4937
Tsp45I GTSAC 6 cut(s) 887, 1945, 3254, 3857, 4162, 4937
TspGWI ACGGA 8 cut(s) 113, 632, 2553, 2843, 4848, 5079, 5144, 5228
Van91I CCANNNNNTGG 3 cut(s) 1099, 3181, 5562
Vha464I CTTAAG 1 cut(s) 4291
VpaK11BI GGWCC 6 cut(s) 18, 1401, 2328, 3403, 3899, 4702
VspI ATTAAT 1 cut(s) 2847
XbaI TCTAGA 2 cut(s) 3494, 3532
XceI RCATGY 5 cut(s) 1495, 1619, 3235, 3262, 5271
XcmI CCANNNNNNNNNTGG 1 cut(s) 5047
XmaJI CCTAGG 1 cut(s) 197
XmiI GTMKAC 5 cut(s) 716, 2814, 2824, 5301, 5407
XmnI GAANNNNTTC 3 cut(s) 1799, 3300, 4117
ZraI GACGTC 2 cut(s) 1582, 4580
Zsp2I ATGCAT 4 cut(s) 687, 1672, 2001, 5002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.