AT3G59100

Vta1 like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009173
Physical Location & Seq
Forward (+)
21842964 .. 21854117
11154 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G59100.2

Sequence Viewer

Length: 5766 bp
ATGGAAGCTAGTTCGAGTGGAACGGCGGAGTTGCCGCGGTCTCTTTCTCGGAGAGCTCCGTCGCGTGCGACTACTATGATGATTGATAGACCAAACGAAGATGCTAGTGCTATGGATAGCGAGCTTGTTCCTTCCTCTCTCGCTTCTATTGCCCCGATTCTTCGCGTTGCTAATGAAATTGAGAAGGACAATCCTCGAGTGGCTTATCTATGCCGGTTTCATGCGTTTGAGAAAGCTCATAGGATGGATGCTACATCCAGTGGACGAGGTGTTCGTCAGTTTAAGACCTATCTGTTACATAGACTTGAGAAGGAAGAAGAGGAAACCAAGCCTCAGCTGGCGAAAAACGATCCTAGAGAAATTCAGGCATATTACCAGAACTTCTATGAGAAATATATTAAAGAAGGAGAAACATCAAGAAAACCGGAGGAAATGGCAAGGCTTTACCAAATTGCATCGGTATTGTACGATGTACTGAAGACTGTAGTACCTTCACCTAAAGTGGATTACGAGACCCGTAGATATGCCGAAGAAGTTGAAAGGAAAAGGGATAGATATGAGCACTACAACATTCTTCCTTTATATGCTGTAGGGACTAAACCAGCAATTGTGGAACTTCCTGAGGTCAAGGCTGCATTCAGTGCTGTCCGTAATGTGCGCAATCTTCCACGACGAAGAATTCATTTGCCATCTAATACCCCTAATGAAATGCGTAAGGCTAGAACCAAACTTAATGACATACTTGAATGGCTTGCTTCTGAGTTCGGGTTTCAGAGAGGGAACGTGGCAAATCAGAGGGAGCACATAATACTGCTTCTTGCTAATGCTGACATTAGAAAGAGAAATGATGAAGAATATGATGAGTTGAAACCTAGCACCGTCACTGAGTTGATGGACAAAACTTTTAAAAGTTATTACTCATGGTGTAAATATTTGCACTCTACGTCTAATCTCAAGTTTCCAGATGATTGTGATAAACAGCAGCTGCAGCTTATCTATATTTCCCTGTACCTTTTAATATGGGGTGAAGCCTCGAATGTTAGATTTATGCCCGAATGTATCTGCTATATTTTCCATAATATGGCAAATGACGTCTATGGAATTCTGTTTAGCAATGTTGAAGCTGTTAGTGGTGAGACATATGAAACAGAAGAAGTTATTGATGAAGAATCATTTCTGAGAACTGTTATAACTCCAATCTATCAGGTCATACGCAACGAAGCCAAAAGGAACAAGGGAGGTACAGCAAGCCATTCGCAATGGAGAAATTACGATGATTTGAATGAATACTTTTGGTCTAAGAAATGCTTCAAGATAGGATGGCCTCTTGACCTTAAAGCAGACTTTTTCCTAAACTCTGACGAGATAACCCCACAGGACGAGAGGCTGAATCAAGTCACTTATGGGAAAAGCAAGCCTAAAACCAACTTTGTTGAAGTCCGCACATTCTGGAACCTCTTCAGAGATTTTGATCGAATGTGGATATTTCTTGTCATGGCTTTTCAGGCTATGGTAATAGTTGGATGGCATGGTTCTGGGTCACTAGGTGACATATTTGACAAGGATGTATTCAAAACGGTGTTAACTATTTTTATTACTTCGGCGTATCTGACTCTTTTACAAGCTGCACTGGATATTATCCTAAATTTCAATGCCTGGAAGAATTTCAAGTTTTCCCAGATATTGCGGTACCTTTTGAAATTCGCAGTAGCATTTATGTGGGCGGTACTGCTTCCAATTGCTTACTCGAAGTCTGTTCAACGCCCAACTGGAGTTGTAAAGTTCTTCAGTACTTGGACTGGGGATTGGAAGGATCAGTCCTTTTATACGTACGCTGTTTCATTTTATGTGTTACCAAACATATTGGCAGCTCTGTTATTCCTCGTTCCACCATTCCGGAGAGCCATGGAGTGTTCAGACATGCGACCTATCAAAGTTATAATGTGGTGGGCTCAGCCAAAACTATACGTTGGAAGAGGTATGCATGAGGACATGTTCTCTCTCTTCAAGTATACCACCTTCTGGATCATGCTACTGATCAGCAAGCTTGCTTTCAACTATTATGTGGAGATACTACCCCTTATTACGCCAACAAAGATGATCATGAATCTGCACATTGGTCACTACCAGTGGCATGAATTTTTTCCCCATGCAACTAACAATATTGGAGTGGTTATTGCTATATGGGCTCCAATTGTACTGGTTTACTTGATGGATACTCAAATATGGTACGCCATTTTTTCCACTCTGTTTGGAGGCATACATGGGGCCTTCAGTCATCTGGGAGAAATACGGACTCTTGGGATGTTGCGATCTAGATTTGAGTCAATACCAATTGCCTTCAGCCGCACCCTCATGCCATCCGAAGATGCAAAAAGAAAACATGCGGATGACTATGTGGACCAGAAAAACATCACTAATTTCTCCCAGGTGTGGAACGAGTTCATTTACTCTATGAGAAGCGAGGATAAGATCAGTGACAGAGATAGGGATTTGCTGCTTGTACCATCTTCGTCGGGTGATGTGTCTGTCATCCAGTGGCCACCTTTCTTGCTTGCTAGTAAGATTCCTATTGCTGTGGACATGGCAAAAGATTTCAAGGGAAAAGAGGATGCTGAACTATTTAGAAAGATTAAGAGTGATAGCTACATGTATTATGCTGTTATTGAGAGTTATGAGACATTGAAGAAAATAATTTACGCTCTTCTGGAAGATGAAGCAGATAGAAGGGTCATGAATCAGGTATTTTTAGAAGTGGACATGAGCATGCAGCAACAGAGATTCATATATGAATTCCGCATGAGTGGATTACCGCTCCTTAGTGATAAGCTGGAGAAGTTTCTAAGTATATTGTTAAGTGACTATGAAGACCAGGGTACATACAAGTCTCAGTTAATTAATGTCTTCCAGGATGTTATCGAAATTATCACTCAAGATCTCTTGGTTAATGGGCATGAAATTCTTGAAAGGGCTCGTGTTCATTCTCCTGACATCAAAAATGAGAAAAAGGAACAGAGGTTTGAGAAAATTAATATCCACCTAGTACGGGATAGATGTTGGCGGGAAAAGGTTATTCGGCTTCATTTGCTCTTATCGGTCAAAGAATCTGCAATAAATGTTCCTCAAAACTTGGAAGCCCGCCGACGTATCACATTCTTTGCTAATTCTCTGTTTATGAATATGCCTAGTGCTCCAAGAATACGAGATATGCTATCTTTCAGTGTTTTAACTCCTTACTACAAAGAAGATGTGCTGTATTCAGAGGAAGACCTTAACAAGGAAAATGAAGATGGGATCTCAATTCTGTTTTATCTACAAAAGATTTATCCTGATGAGTGGACCAATTATTTGGATCGCTTAAAGGATCCTAAGCTTCCTGAGAAAGACAAGTCAGAGTTCCTTCGAGAGTGGGTATCTTACAGAGGTCAGACGTTAGCCAGAACAGTTAGGGGTATGATGTACTATAGACAGGCTCTGGAGCTTCAATGCTACCAGGAAGTTGCAGGGGAACAAGCCGAGTTTAGTGTCTTTCGGGCCATGGCGTCCAACGATGAAAATCAGAAGGCTTTCCTGGAACGTGCCAGAGCTCTTGCAGATCTGAAGTTCACTTATGTTGTTTCTTGTCAGGTCTACGGAAATCAGAAAAAGTCCGGTGATATCCACAACCGCAGCTGCTACACGAATATTCTGCAGCTCATGCTAAAATATCCATCATTGCGGGTTGCTTATGTAGATGAGAGGGAGGAGACAGCAGATGCAAAATCTCCGAAGGTCTTTTACTCTGTACTTCTAAAAGGAGGTGACAAGTTTGATGAGGAAATTTACCGCATCAAGCTTCCTGGTCCTCCAGCTGAGATTGGTGAAGGAAAGCCTGAAAATCAAAATCATGCTATCATATTTACACGTGGTGAAGCACTACAAACTATAGATATGAATCAAGACAATTACTTTGAAGAAGCTTTCAAACTAAGAAATGTATTGGAGGAATTCAATAAGGAGCGTGTTGGTAGACGCAAGCCAACAATATTGGGTCTCCGAGAACATATATTTACTGGAAGTGTTTCGTCATTAGCTTGGTTCATGTCCAATCAGGAGAGTAGTTTTGTGACAATTGGCCAAAGAATTTTGGCAAACCCTCTGAGGGTCCGTTTTCATTATGGTCATCCTGACATATTTGACAGAATCTTTCACATAACAAGGGGCGGTGTGAGCAAGGCTTCGAAAGTAATAAATTTGAGTGAGGATATTTTCGGAGGCTTCAATTCAACTCTCCGTGGAGGATATGTTACACATCACGAGTATATCCAAGTTGGAAAGGGACGGGACGTGGGGTTGAACCCAATCTCTATTTTTGAAGCAAAAGTAGCAAATGGAAACGGGGAGCAAACGCTAAGCCGTGATGTTTACCGTTTGGGGCATCGGTTTGACTTTTACAGGATGCTCTCATTCTATTTTACGACCATTGGATTCTATTTTAGTAGCATGCTCACCGTTCTTACTGTATATGCATTCCTGTATGGACGGATGTACATGGTTATGAGCGGATTGGAGAAAGAGATTTTGAGACTCGCAAGCCCGAATCAGCTTGAGGCTCTTGAGCAGGCTCTGGCTACACAGTCAATATTTCAGCTTGGTTTCTTGATGGTGTTACCGATGGTAATGGAGATTGGCTTGGAACACGGTTTTCGCTCTGCTATTGTCGATTTTTTCATAATGCAGCTGCAGCTAGCCTCGGTATTCTTTACATTCCAGCTTGGGACAAAGTCACATTATTACGGGAGAACAATTTTACATGGTGGTTCCAAATATAGACCGACGGGCCGTGGGTTTGTTGTTTTCCATGCCAAGTTTGCGGAGAACTACAGGTTGTATTCGAGAAGTCACTTTGTGAAGGGACTAGAGCTGCTTTTATTGCTAGTTGTTTATCAAATCTATGGGCATTCATACCGGAGTTCAAACCTTTATCTGTATATCACAGTCTCGATGTGGTTTATGGTTGGTTCTTGGTTGTTTGCACCATTTATATTCAATCCTTCGGGGTTTGAGTGGCAAAAGACAGTTGACGATTGGACTGATTGGAAACGATGGCTTGGAGATCGGGGTGGTATAGGCATCCCGGTTGAAAAAAGCTGGGAATCGTGGTGGAACGTAGAGCAAGAACACCTCAAACATACAAGTATTAGAGGGAGGATTCTGGAAATCACACTTGCTCTCCGCTTTTTCATTTATCAGTACGGAATTGTTTACCAGCTCAATATCTCTCAGCGCAGCAAGAGCTTTTTGGTTTATGGACTCTCTTGGGTGGTCTTGCTCACATCATTACTTGTTCTAAAGATGGTATCTATGGGCAGACGAAGATTTGGAACAGATTTTCAGCTAATGTTCAGGATTCTTAAAGCACTTCTCTTCCTCGGCTTTTTGTCAGTCATGACAATATTATTTGTGGTGTTCAAACTCACACTCACCGATCTCTCTGCTTCAGTTCTTGCTTTTCTTCCAACCGGGTGGGCGATTCTTCTGATTGGGCAAGTACTGAGGTCACCGATCAAAGCTTTAGGGGTTTGGGACTCGGTGAAAGAGCTAGGAAGAGCGTATGAGAATATCATGGGACTTGTAATCTTTGCACCAATCGCTGTTCTGTCTTGGTTTCCCATTGTCTCCGAGTTTCAAGCTCGTCTGCTTTTCAATCAAGCTTTTAGCAGAGGCCTTCAAATCTCTATGATTCTCGCTGGTCGGAAAGACAAAGCAACTTCTTCGCACAAGTAA

Protein Analysis

1921

Amino Acids

223.51

Weight (kDa)

8.76

Isoelectric Point (pI)

40.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FKS1_dom1 PF14288 33 - 145 3.4e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 551 - 736 2.9e-64 Callose synthase 1 domain
Glucan_synthase PF02364 743 - 825 4.9e-28 1,3-beta-glucan synthase component
Glucan_synthase PF02364 836 - 1435 8.7e-203 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1190, 1940
AatII GACGTC 1 cut(s) 1095
Acc16I TGCGCA 1 cut(s) 659
Acc65I GGTACC 1 cut(s) 1689
AccB1I GGYRCC 1 cut(s) 1689
AccB7I CCANNNNNTGG 2 cut(s) 1081, 2022
AccBSI CCGCTC 2 cut(s) 2815, 4542
AccI GTMKAC 3 cut(s) 2012, 3630, 4009
AccII CGCG 3 cut(s) 37, 64, 165
AccIII TCCGGA 1 cut(s) 1896
AclWI GGATC 7 cut(s) 344, 1821, 2033, 3302, 3359, 3360, 3372
AcoI YGGCCR 2 cut(s) 2540, 4114
AcuI CTGAAG 7 cut(s) 497, 1444, 1771, 2257, 2326, 3620, 5463
AcvI CACGTG 1 cut(s) 3905
AcyI GRCGYC 2 cut(s) 1092, 3542
AdeI CACNNNGTG 3 cut(s) 1547, 3908, 4888
AfiI CCNNNNNNNGG 6 cut(s) 1081, 2022, 2433, 3046, 3277, 4141
AflIII ACRYGT 3 cut(s) 1992, 2649, 3902
AjiI CACGTC 1 cut(s) 4327
AjnI CCWGG 7 cut(s) 1655, 2427, 2871, 2907, 3492, 3570, 3838
AloI GAACNNNNNNTCC 2 cut(s) 255, 287
Alw21I GWGCWC 5 cut(s) 58, 564, 804, 3193, 3589
AlwI GGATC 7 cut(s) 344, 1821, 2033, 3302, 3359, 3360, 3372
AlwNI CAGNNNCTG 3 cut(s) 985, 3475, 4606
Ama87I CYCGRG 1 cut(s) 195
Aor13HI TCCGGA 1 cut(s) 1896
AoxI GGCC 7 cut(s) 1322, 2268, 2540, 3534, 4114, 4819, 5704
ArsI GACNNNNNNTTYG 6 cut(s) 255, 287, 3331, 3363, 3932, 3964
AseI ATTAAT 2 cut(s) 2898, 3030
Asp700I GAANNNNTTC 8 cut(s) 1173, 1307, 1457, 1664, 2142, 2441, 3566, 4060
Asp718I GGTACC 1 cut(s) 1689
AspLEI GCGC 2 cut(s) 660, 5268
AspS9I GGNCC 7 cut(s) 2268, 2401, 3339, 3534, 3842, 4144, 4819
AsuC2I CCSGG 2 cut(s) 5117, 5503
AsuII TTCGAA 1 cut(s) 4220
AsuNHI GCTAGC 1 cut(s) 4726
AvaI CYCGRG 1 cut(s) 195
AvaII GGWCC 4 cut(s) 2401, 3339, 3842, 4144
AxyI CCTNAGG 1 cut(s) 621
BaeI ACNNNNGTAYC 2 cut(s) 471, 504
BalI TGGCCA 2 cut(s) 2542, 4116
BamHI GGATCC 1 cut(s) 3364
BanI GGYRCC 1 cut(s) 1689
BanII GRGCYC 5 cut(s) 58, 1954, 2191, 2974, 3589
BarI GAAGNNNNNNTAC 4 cut(s) 2003, 2035, 3761, 3793
BauI CACGAG 2 cut(s) 2973, 4295
BbrPI CACGTG 1 cut(s) 3905
BbsI GAAGAC 4 cut(s) 485, 2874, 2896, 3273
Bbv12I GWGCWC 5 cut(s) 58, 564, 804, 3193, 3589
BbvCI CCTCAGC 1 cut(s) 333
BceAI ACGGC 3 cut(s) 39, 4380, 4806
BcgI CGANNNNNNTGC 7 cut(s) 1236, 1270, 3670, 3704, 5456, 5490, 5736
BciT130I CCWGG 7 cut(s) 1657, 2429, 2873, 2909, 3494, 3572, 3840
BciVI GTATCC 1 cut(s) 2209
BclI TGATCA 2 cut(s) 2037, 2100
BcnI CCSGG 2 cut(s) 5117, 5503
BfmI CTRYAG 8 cut(s) 483, 588, 986, 3463, 3689, 3924, 4721, 4861
BfuI GTATCC 1 cut(s) 2209
BglII AGATCT 2 cut(s) 2935, 3595
BlpI GCTNAGC 2 cut(s) 1953, 4391
BmcAI AGTACT 2 cut(s) 1792, 5532
Bme1390I CCNGG 9 cut(s) 1657, 2429, 2873, 2909, 3494, 3572, 3840, 5117, 5503
Bme18I GGWCC 4 cut(s) 2401, 3339, 3842, 4144
BmeT110I CYCGRG 1 cut(s) 195
BmgBI CACGTC 1 cut(s) 4327
BmgT120I GGNCC 7 cut(s) 2268, 2401, 3339, 3534, 3842, 4144, 4819
BmiI GGNNCC 7 cut(s) 1454, 1691, 2190, 2269, 3366, 4145, 4801
BmrFI CCNGG 9 cut(s) 1657, 2429, 2873, 2909, 3494, 3572, 3840, 5117, 5503
BmrI ACTGGG 1 cut(s) 1809
BmtI GCTAGC 1 cut(s) 4730
BmuI ACTGGG 1 cut(s) 1809
BpiI GAAGAC 4 cut(s) 485, 2874, 2896, 3273
BpmI CTGGAG 4 cut(s) 1791, 2852, 3497, 3831
Bpu10I CCTNAGC 2 cut(s) 333, 3369
Bpu1102I GCTNAGC 2 cut(s) 1953, 4391
Bpu14I TTCGAA 1 cut(s) 4220
BpuEI CTTGAG 5 cut(s) 326, 938, 2916, 4607, 4616
BpuMI CCSGG 2 cut(s) 5117, 5503
BsaAI YACGTR 2 cut(s) 1830, 3905
BsaBI GATNNNNATC 3 cut(s) 1470, 3555, 3933
BsaHI GRCGYC 2 cut(s) 1092, 3542
BsaI GGTCTC 3 cut(s) 45, 506, 4037
BsaJI CCNNGG 9 cut(s) 35, 1905, 2427, 2872, 3537, 4273, 4731, 4822, 5410
BsaWI WCCGGW 4 cut(s) 424, 1896, 3650, 4947
BsaXI ACNNNNNCTCC 4 cut(s) 4269, 4299, 5196, 5226
Bsc4I CCNNNNNNNGG 6 cut(s) 1081, 2022, 2433, 3046, 3277, 4141
Bse118I RCCGGY 1 cut(s) 213
Bse1I ACTGG 8 cut(s) 258, 1635, 1774, 1804, 2128, 2205, 2536, 4057
Bse21I CCTNAGG 1 cut(s) 621
Bse3DI GCAATG 3 cut(s) 1120, 1265, 3713
Bse8I GATNNNNATC 3 cut(s) 1470, 3555, 3933
BseAI TCCGGA 1 cut(s) 1896
BseBI CCWGG 7 cut(s) 1657, 2429, 2873, 2909, 3494, 3572, 3840
BseDI CCNNGG 9 cut(s) 35, 1905, 2427, 2872, 3537, 4273, 4731, 4822, 5410
BseJI GATNNNNATC 3 cut(s) 1470, 3555, 3933
BseLI CCNNNNNNNGG 6 cut(s) 1081, 2022, 2433, 3046, 3277, 4141
BseMI GCAATG 3 cut(s) 1120, 1265, 3713
BseNI ACTGG 8 cut(s) 258, 1635, 1774, 1804, 2128, 2205, 2536, 4057
BseRI GAGGAG 1 cut(s) 3758
BseYI CCCAGC 1 cut(s) 5130
BsgI GTGCAG 2 cut(s) 1611, 2096
Bsh1236I CGCG 3 cut(s) 37, 64, 165
BshFI GGCC 7 cut(s) 1324, 2270, 2542, 3536, 4116, 4821, 5706
BshNI GGYRCC 1 cut(s) 1689
BsiHKAI GWGCWC 5 cut(s) 58, 564, 804, 3193, 3589
BsiHKCI CYCGRG 1 cut(s) 195
BsiSI CCGG 7 cut(s) 214, 425, 1897, 3651, 4948, 5117, 5502
BsiWI CGTACG 1 cut(s) 1830
BslFI GGGAC 7 cut(s) 607, 4332, 4337, 4771, 4908, 5579, 5622
BslI CCNNNNNNNGG 6 cut(s) 1081, 2022, 2433, 3046, 3277, 4141
BsmFI GGGAC 7 cut(s) 607, 4332, 4337, 4771, 4908, 5579, 5622
BsmI GAATGC 3 cut(s) 635, 4508, 4939
BsnI GGCC 7 cut(s) 1324, 2270, 2542, 3536, 4116, 4821, 5706
Bso31I GGTCTC 3 cut(s) 45, 506, 4037
BsoBI CYCGRG 1 cut(s) 195
Bsp119I TTCGAA 1 cut(s) 4220
Bsp1286I GDGCHC 8 cut(s) 58, 564, 804, 1954, 2191, 2974, 3193, 3589
Bsp13I TCCGGA 1 cut(s) 1896
Bsp1407I TGTACA 1 cut(s) 4527
Bsp1720I GCTNAGC 2 cut(s) 1953, 4391
Bsp19I CCATGG 2 cut(s) 1905, 3537
BspANI GGCC 7 cut(s) 1324, 2270, 2542, 3536, 4116, 4821, 5706
BspEI TCCGGA 1 cut(s) 1896
BspFNI CGCG 3 cut(s) 37, 64, 165
BspHI TCATGA 3 cut(s) 2103, 2733, 5427
BspLI GGNNCC 7 cut(s) 1454, 1691, 2190, 2269, 3366, 4145, 4801
BspMAI CTGCAG 3 cut(s) 990, 3693, 4725
BspOI GCTAGC 1 cut(s) 4730
BspPI GGATC 7 cut(s) 344, 1821, 2033, 3302, 3359, 3360, 3372
BspQI GCTCTTC 2 cut(s) 2709, 5581
BspT104I TTCGAA 1 cut(s) 4220
BspT107I GGYRCC 1 cut(s) 1689
BspTNI GGTCTC 3 cut(s) 45, 506, 4037
BsrBI CCGCTC 2 cut(s) 2815, 4542
BsrDI GCAATG 3 cut(s) 1120, 1265, 3713
BsrFI RCCGGY 1 cut(s) 213
BsrGI TGTACA 1 cut(s) 4527
BsrI ACTGG 8 cut(s) 258, 1635, 1774, 1804, 2128, 2205, 2536, 4057
BssAI RCCGGY 1 cut(s) 213
BssECI CCNNGG 9 cut(s) 35, 1905, 2427, 2872, 3537, 4273, 4731, 4822, 5410
BssNAI GTATAC 1 cut(s) 2013
BssNI GRCGYC 2 cut(s) 1092, 3542
BssSI CACGAG 2 cut(s) 2973, 4295
BssT1I CCWWGG 2 cut(s) 1905, 3537
Bst1107I GTATAC 1 cut(s) 2013
Bst2BI CACGAG 2 cut(s) 2973, 4295
Bst2UI CCWGG 7 cut(s) 1657, 2429, 2873, 2909, 3494, 3572, 3840
Bst6I CTCTTC 7 cut(s) 312, 1463, 1969, 2009, 2709, 5411, 5581
BstACI GRCGYC 2 cut(s) 1092, 3542
BstAPI GCANNNNNTGC 2 cut(s) 641, 3697
BstAUI TGTACA 1 cut(s) 4527
BstBAI YACGTR 2 cut(s) 1830, 3905
BstBI TTCGAA 1 cut(s) 4220
BstDSI CCRYGG 5 cut(s) 35, 1905, 3537, 4273, 4822
BstEII GGTNACC 1 cut(s) 5538
BstENI CCTNNNNNAGG 1 cut(s) 3275
BstFNI CGCG 3 cut(s) 37, 64, 165
BstHHI GCGC 2 cut(s) 660, 5268
BstNI CCWGG 7 cut(s) 1657, 2429, 2873, 2909, 3494, 3572, 3840
BstNSI RCATGY 6 cut(s) 1924, 1996, 2387, 2653, 2770, 4486
BstPI GGTNACC 1 cut(s) 5538
BstSCI CCNGG 9 cut(s) 1655, 2427, 2871, 2907, 3492, 3570, 3838, 5115, 5501
BstSFI CTRYAG 8 cut(s) 483, 588, 986, 3463, 3689, 3924, 4721, 4861
BstSNI TACGTA 1 cut(s) 1830
BstUI CGCG 3 cut(s) 37, 64, 165
BstV2I GAAGAC 4 cut(s) 485, 2874, 2896, 3273
BstX2I RGATCY 4 cut(s) 2935, 3294, 3364, 3595
BstXI CCANNNNNNTGG 2 cut(s) 3349, 5505
BstYI RGATCY 4 cut(s) 2935, 3294, 3364, 3595
BstZ17I GTATAC 1 cut(s) 2013
Bsu36I CCTNAGG 1 cut(s) 621
BsuI GTATCC 1 cut(s) 2209
BsuRI GGCC 7 cut(s) 1324, 2270, 2542, 3536, 4116, 4821, 5706
BtgI CCRYGG 5 cut(s) 35, 1905, 3537, 4273, 4822
BtrI CACGTC 1 cut(s) 4327
BtsIMutI CAGTG 8 cut(s) 265, 646, 882, 1628, 2135, 2482, 2543, 3226
CaiI CAGNNNCTG 3 cut(s) 985, 3475, 4606
CciI TCATGA 3 cut(s) 2103, 2733, 5427
CfoI GCGC 2 cut(s) 660, 5268
Cfr10I RCCGGY 1 cut(s) 213
Cfr13I GGNCC 7 cut(s) 2268, 2401, 3339, 3534, 3842, 4144, 4819
Cfr42I CCGCGG 1 cut(s) 38
CseI GACGC 2 cut(s) 3531, 4020
CspCI CAANNNNNGTGG 4 cut(s) 2233, 2268, 2532, 2567
DraI TTTAAA 1 cut(s) 907
DraIII CACNNNGTG 3 cut(s) 1547, 3908, 4888
EaeI YGGCCR 2 cut(s) 2540, 4114
Eam1104I CTCTTC 7 cut(s) 312, 1463, 1969, 2009, 2709, 5411, 5581
EarI CTCTTC 7 cut(s) 312, 1463, 1969, 2009, 2709, 5411, 5581
EciI GGCGGA 1 cut(s) 41
Ecl136II GAGCTC 2 cut(s) 56, 3587
Eco105I TACGTA 1 cut(s) 1830
Eco130I CCWWGG 2 cut(s) 1905, 3537
Eco147I AGGCCT 1 cut(s) 5706
Eco24I GRGCYC 5 cut(s) 58, 1954, 2191, 2974, 3589
Eco31I GGTCTC 3 cut(s) 45, 506, 4037
Eco32I GATATC 1 cut(s) 3658
Eco47I GGWCC 4 cut(s) 2401, 3339, 3842, 4144
Eco53kI GAGCTC 2 cut(s) 56, 3587
Eco57I CTGAAG 7 cut(s) 497, 1444, 1771, 2257, 2326, 3620, 5463
Eco72I CACGTG 1 cut(s) 3905
Eco81I CCTNAGG 1 cut(s) 621
Eco88I CYCGRG 1 cut(s) 195
Eco91I GGTNACC 1 cut(s) 5538
EcoICRI GAGCTC 2 cut(s) 56, 3587
EcoNI CCTNNNNNAGG 1 cut(s) 3275
EcoO109I RGGNCCY 1 cut(s) 2268
EcoO65I GGTNACC 1 cut(s) 5538
EcoRI GAATTC 4 cut(s) 678, 1101, 2792, 3986
EcoRII CCWGG 7 cut(s) 1655, 2427, 2871, 2907, 3492, 3570, 3838
EcoRV GATATC 1 cut(s) 3658
EcoT14I CCWWGG 2 cut(s) 1905, 3537
EcoT22I ATGCAT 2 cut(s) 1986, 4510
EcoT38I GRGCYC 5 cut(s) 58, 1954, 2191, 2974, 3589
ErhI CCWWGG 2 cut(s) 1905, 3537
FalI AAGNNNNNCTT 2 cut(s) 1292, 1324
FaqI GGGAC 7 cut(s) 607, 4332, 4337, 4771, 4908, 5579, 5622
FauI CCCGC 3 cut(s) 3054, 3146, 3711
FauNDI CATATG 1 cut(s) 1141
FbaI TGATCA 2 cut(s) 2037, 2100
FblI GTMKAC 3 cut(s) 2012, 3630, 4009
FriOI GRGCYC 5 cut(s) 58, 1954, 2191, 2974, 3589
FspI TGCGCA 1 cut(s) 659
GlaI GCGC 2 cut(s) 659, 5267
GsaI CCCAGC 1 cut(s) 5134
GsuI CTGGAG 4 cut(s) 1791, 2852, 3497, 3831
HaeIII GGCC 7 cut(s) 1324, 2270, 2542, 3536, 4116, 4821, 5706
HapII CCGG 7 cut(s) 214, 425, 1897, 3651, 4948, 5117, 5502
HgaI GACGC 2 cut(s) 3531, 4020
HhaI GCGC 2 cut(s) 660, 5268
Hin1I GRCGYC 2 cut(s) 1092, 3542
Hin6I GCGC 2 cut(s) 658, 5266
HinP1I GCGC 2 cut(s) 658, 5266
HincII GTYRAC 2 cut(s) 1584, 5062
HindII GTYRAC 2 cut(s) 1584, 5062
HindIII AAGCTT 6 cut(s) 2045, 3371, 3833, 3957, 5550, 5691
HpaI GTTAAC 1 cut(s) 1584
HpaII CCGG 7 cut(s) 214, 425, 1897, 3651, 4948, 5117, 5502
Hpy99I CGWCG 5 cut(s) 64, 675, 2518, 3147, 4819
Hsp92I GRCGYC 2 cut(s) 1092, 3542
HspAI GCGC 2 cut(s) 658, 5266
Kpn2I TCCGGA 1 cut(s) 1896
KpnI GGTACC 1 cut(s) 1693
Ksp22I TGATCA 2 cut(s) 2037, 2100
KspAI GTTAAC 1 cut(s) 1584
KspI CCGCGG 1 cut(s) 38
LguI GCTCTTC 2 cut(s) 2709, 5581
LmnI GCTCC 8 cut(s) 61, 799, 2194, 2820, 3196, 3478, 3997, 4381
MbiI CCGCTC 2 cut(s) 2815, 4542
MfeI CAATTG 5 cut(s) 606, 1737, 2193, 2334, 4110
MflI RGATCY 4 cut(s) 2935, 3294, 3364, 3595
MhlI GDGCHC 8 cut(s) 58, 564, 804, 1954, 2191, 2974, 3193, 3589
MlsI TGGCCA 2 cut(s) 2542, 4116
MluNI TGGCCA 2 cut(s) 2542, 4116
MlyI GAGTC 6 cut(s) 1606, 2290, 2333, 4560, 5286, 5561
MmeI TCCRAC 6 cut(s) 1501, 1951, 3570, 4291, 5522, 5714
Mox20I TGGCCA 2 cut(s) 2542, 4116
Mph1103I ATGCAT 2 cut(s) 1986, 4510
MroI TCCGGA 1 cut(s) 1896
MroXI GAANNNNTTC 8 cut(s) 1173, 1307, 1457, 1664, 2142, 2441, 3566, 4060
MscI TGGCCA 2 cut(s) 2542, 4116
MslI CAYNNNNRTG 5 cut(s) 1717, 2354, 2388, 2765, 4535
Msp20I TGGCCA 2 cut(s) 2542, 4116
MspA1I CMGCKG 6 cut(s) 37, 337, 985, 3672, 3851, 4720
MspI CCGG 7 cut(s) 214, 425, 1897, 3651, 4948, 5117, 5502
MspR9I CCNGG 9 cut(s) 1657, 2429, 2873, 2909, 3494, 3572, 3840, 5117, 5503
MunI CAATTG 5 cut(s) 606, 1737, 2193, 2334, 4110
Mva1269I GAATGC 3 cut(s) 635, 4508, 4939
MvaI CCWGG 7 cut(s) 1657, 2429, 2873, 2909, 3494, 3572, 3840
MvnI CGCG 3 cut(s) 37, 64, 165
NciI CCSGG 2 cut(s) 5117, 5503
NcoI CCATGG 2 cut(s) 1905, 3537
NdeI CATATG 1 cut(s) 1141
NheI GCTAGC 1 cut(s) 4726
NlaIV GGNNCC 7 cut(s) 1454, 1691, 2190, 2269, 3366, 4145, 4801
NmeAIII GCCGAG 2 cut(s) 3541, 5391
NsbI TGCGCA 1 cut(s) 659
NsiI ATGCAT 2 cut(s) 1986, 4510
NspI RCATGY 6 cut(s) 1924, 1996, 2387, 2653, 2770, 4486
NspV TTCGAA 1 cut(s) 4220
PacI TTAATTAA 1 cut(s) 2898
PaeI GCATGC 2 cut(s) 2770, 4486
PaeR7I CTCGAG 1 cut(s) 195
PagI TCATGA 3 cut(s) 2103, 2733, 5427
PceI AGGCCT 1 cut(s) 5706
PciI ACATGT 2 cut(s) 1992, 2649
PciSI GCTCTTC 2 cut(s) 2709, 5581
PcsI WCGNNNNNNNCGW 1 cut(s) 271
PctI GAATGC 3 cut(s) 635, 4508, 4939
PdmI GAANNNNTTC 8 cut(s) 1173, 1307, 1457, 1664, 2142, 2441, 3566, 4060
Pfl23II CGTACG 1 cut(s) 1830
PflFI GACNNNGTC 1 cut(s) 4762
PflMI CCANNNNNTGG 2 cut(s) 1081, 2022
PfoI TCCNGGA 2 cut(s) 2907, 3570
PleI GAGTC 6 cut(s) 1606, 2290, 2332, 4560, 5286, 5561
PmaCI CACGTG 1 cut(s) 3905
PmlI CACGTG 1 cut(s) 3905
PpsI GAGTC 6 cut(s) 1606, 2290, 2332, 4560, 5286, 5561
Ppu21I YACGTR 2 cut(s) 1830, 3905
PscI ACATGT 2 cut(s) 1992, 2649
PshBI ATTAAT 2 cut(s) 2898, 3030
PsiI TTATAA 2 cut(s) 1190, 1940
Psp124BI GAGCTC 2 cut(s) 58, 3589
Psp6I CCWGG 7 cut(s) 1655, 2427, 2871, 2907, 3492, 3570, 3838
PspCI CACGTG 1 cut(s) 3905
PspEI GGTNACC 1 cut(s) 5538
PspFI CCCAGC 1 cut(s) 5130
PspGI CCWGG 7 cut(s) 1655, 2427, 2871, 2907, 3492, 3570, 3838
PspLI CGTACG 1 cut(s) 1830
PspN4I GGNNCC 7 cut(s) 1454, 1691, 2190, 2269, 3366, 4145, 4801
PspPI GGNCC 7 cut(s) 2268, 2401, 3339, 3534, 3842, 4144, 4819
PspXI VCTCGAGB 1 cut(s) 195
PstI CTGCAG 3 cut(s) 990, 3693, 4725
PstNI CAGNNNCTG 3 cut(s) 985, 3475, 4606
PsuI RGATCY 4 cut(s) 2935, 3294, 3364, 3595
PsyI GACNNNGTC 1 cut(s) 4762
PvuII CAGCTG 5 cut(s) 337, 985, 3672, 3851, 4720
RseI CAYNNNNRTG 5 cut(s) 1717, 2354, 2388, 2765, 4535
SacI GAGCTC 2 cut(s) 58, 3589
SacII CCGCGG 1 cut(s) 38
SapI GCTCTTC 2 cut(s) 2709, 5581
Sau96I GGNCC 7 cut(s) 2268, 2401, 3339, 3534, 3842, 4144, 4819
ScaI AGTACT 2 cut(s) 1792, 5532
SchI GAGTC 6 cut(s) 1606, 2290, 2333, 4560, 5286, 5561
ScrFI CCNGG 9 cut(s) 1657, 2429, 2873, 2909, 3494, 3572, 3840, 5117, 5503
SduI GDGCHC 8 cut(s) 58, 564, 804, 1954, 2191, 2974, 3193, 3589
SfcI CTRYAG 8 cut(s) 483, 588, 986, 3463, 3689, 3924, 4721, 4861
Sfr274I CTCGAG 1 cut(s) 195
Sfr303I CCGCGG 1 cut(s) 38
SfuI TTCGAA 1 cut(s) 4220
SgrBI CCGCGG 1 cut(s) 38
SinI GGWCC 4 cut(s) 2401, 3339, 3842, 4144
SlaI CTCGAG 1 cut(s) 195
SmiMI CAYNNNNRTG 5 cut(s) 1717, 2354, 2388, 2765, 4535
SmlI CTYRAG 6 cut(s) 195, 305, 953, 2931, 4586, 4595
SmoI CTYRAG 6 cut(s) 195, 305, 953, 2931, 4586, 4595
SnaBI TACGTA 1 cut(s) 1830
SphI GCATGC 2 cut(s) 2770, 4486
SseBI AGGCCT 1 cut(s) 5706
SspI AATATT 6 cut(s) 932, 2164, 3685, 4026, 4623, 5436
SstI GAGCTC 2 cut(s) 58, 3589
StuI AGGCCT 1 cut(s) 5706
StyD4I CCNGG 9 cut(s) 1655, 2427, 2871, 2907, 3492, 3570, 3838, 5115, 5501
StyI CCWWGG 2 cut(s) 1905, 3537
TaqII GACCGA 2 cut(s) 3085, 4828
TatI WGTACW 7 cut(s) 472, 1790, 2197, 3459, 3784, 4527, 5530
TauI GCSGC 2 cut(s) 37, 2349
TscAI CASTG 8 cut(s) 265, 646, 889, 1635, 2135, 2482, 2543, 3226
TspGWI ACGGA 8 cut(s) 48, 638, 2308, 3648, 4136, 4262, 4537, 5250
TspRI CASTG 8 cut(s) 265, 646, 889, 1635, 2135, 2482, 2543, 3226
Tth111I GACNNNGTC 1 cut(s) 4762
Van91I CCANNNNNTGG 2 cut(s) 1081, 2022
VpaK11BI GGWCC 4 cut(s) 2401, 3339, 3842, 4144
VspI ATTAAT 2 cut(s) 2898, 3030
XagI CCTNNNNNAGG 1 cut(s) 3275
XbaI TCTAGA 1 cut(s) 2315
XceI RCATGY 6 cut(s) 1924, 1996, 2387, 2653, 2770, 4486
XcmI CCANNNNNNNNNTGG 1 cut(s) 334
XhoI CTCGAG 1 cut(s) 195
XmiI GTMKAC 3 cut(s) 2012, 3630, 4009
XmnI GAANNNNTTC 8 cut(s) 1173, 1307, 1457, 1664, 2142, 2441, 3566, 4060
ZraI GACGTC 1 cut(s) 1093
ZrmI AGTACT 2 cut(s) 1792, 5532
Zsp2I ATGCAT 2 cut(s) 1986, 4510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.