Rroxscaffold_2G00096190

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009173
Physical Location & Seq
Forward (+)
17431631 .. 17447758
16128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00096190.1

Sequence Viewer

Length: 5439 bp
ATGGCGAGCTCCAGCGGGACCAAGGCCGATGGGTGGCCGCAGAGGTCGCTGTCGAGGGGAATGACGAGAATGGCTACTAGAGTTGTGGACTTTCCGACGGAGGGCGACGATGCGTTGGACAGTGGAGTTGTGCCGTCGTGCCTTGCGTCTATTGCTCCGATTTTCCGGGTTGCCAATGAGATTGAGAAGGACAACCCTAGGGTTGCTTATCTCTGCCGCTTCCATGGATTTGAAAAGGCGCATACCATGGATCCAACATCAAGTGGACGTGGAGTTCGTCAGTTTAAGACACATCTTTTGCACAGACTTGAAAGGGACGAAGTAGATACACAACATCAGCTTGCTAAATCTGATACAAAAGAAATCATGTTGTATTACCACCAATTCTATGAGAAAAATATCAGAGAGGGAGAATATACAAAGAAACCGGAAGAGATGGCCAGGATTTGTCAGATTGCAACTGTACTGTATGATGTGCTCAAGACAGTGGTACCTGCCTCTCAAATAGACATACAGACCCAGAGAATTGCTGAAGATGTCAAGAGGAAAAGGGAGCAATATGTAAACTATAACATTCTTCCATTATATACTGTCGGGGTTAAACCGACAATTATGGAACTTCCTGAGATCAAAGCAGCGCTCCGTGCTCTACAGAATGTGAATAATCTTCCAATGCCAAGAATGCATTTTAAACCCACGAATCCTGATGACAAGTCGACAATGCCTACAGAGAGGATTAAACCTGTGAATGACATACTCGATTGGCTTTCGGCTATTTTTGGGTTTCAGAAAGGAAATGTGGCAAATCAGAGGGAACATTTAATTTTGCTACTTGCCAACATTGATGTAAGACATAGAAGTCCTGAGAACTACAATGAGTTGAATAGTGGCACTGTACAGCATTTGATGGAGAAATTCTTTAAGAATTATCGTTCATGGTATAACTATCTGCATTGTAAAACAAATCTTCGGTTCCCCGAAGGTTCTCACAGCAAACAACAATTGGAACTTATATACATTGCTCTCTATCTTCTCATATGGGGTGAAGCTTCAAATGTCCGATTCATGCCTGAATGCTTATGCTACATTTTCCATAATATGGCGAACGAGGTCTATGGAATTTTAAATAGCAATGCTCATACTGTTAGTGGAGAAACATACCAAACAGTAGCACGTGATGAAGAATCTTTTTTGAGGGAAGTTATAACTCCTATCTACCAGGTTTTGTACAAGGAAGCAAAGAGAAACAAAATGGGCAAGGCAAGTCATTCAAGATGGAGAAATTATGATGATTTGAATGAATACTTCTGGTCGGACAAATGTTTTAAGCTAGGGTGGCCAATGGATGCTAAAGCAGATTTCTTTAGGCATTCAGATGGGATACAACCAGTAAATGAGAGGACCAATCAAGCTACTGGTGGTAGGAGGAAGCCTAAAACAAATTTTGTAGAAGTCCGAACATTTTTACACCTCTACAGAAGTTTTGATCGAATGTGGATATTCTTCATATTGGCTTTACAGGCTATGATTATTATTGCATGGAGTCCTTCTGGAGCTATCACTTCAATATTTGATGCGGATGTCTTCAGAAGTGTTTTAAGCATCTTCATGACATGTGCTTTCCTTAATTTATTACAAGCCACTTTGGACATAATTCTCAGTTGGAATGCATGGAAGAGCTTGAAAGTCACTCAAATATTACGATACCTCTTGAAATTTGCAGTAGCTGTTGTCTGGGCTGTGGTTCTGCCTATTGGTTATTCTAGTTCTATGCAGAATCCTACAGGGCTTCTGAAGTTCTTCAGTAGTTGGAGTCGGGATTGGCGGAATCAGTCATTTTATAATTATGCTGTTGCAATCTATATGGTTCCTAATTTATTGGCTGCTATGCTATTTTTTCTTCCACCTTTACGAAGACACATTGAGCGTTCCAACTGGCGCATTATATTGCCGCTTGTTGCACCAACAAAGTTGATAATGGATATGCCTATCAGTAATTACCAATGGCATGAGTTCTTTCCGAATGCAACTCATAATATTGGTGTTGTCATTGCAATATGGACTCCAATTATCCTGGTATATTTTATGGATGCACAAATATGGTATGCAATATTTTCTACTCTGTTTGGTGGGATTCACGGAGCCTTCAGCCATTTGGGGGAGCTTGGGTTTGAGGATTATTCATTTGTTGTCTCTCAGATACGAACACTTGGGATGTTGCGGTCCAGATTTGAGTCTGTGCCTTCAGCTTTCAGTGATCGGCTTATGCCATCGTCAGAGAATAAGGATGCCAGAAAGAAGAAACACTTGGTGGATGAAGGACTTGTTCGAAAGAACATTGCTAATTTCTCTCAGGTCTGGAATGAATTTATAAATTCTATGCGAATGGAAGACCTGATCAGCAATAGGGATAGGGATCTGCTGCTTGTTCCGTATTCTTCAAGTGATGTTTCTGTTGTTCAGTGGCCTCCTTTCTTGCTTGCTAGCAAGATTCCAATAGCATTAGACATGGCAAAGGATTTTACAGGGAAGTCGGATGATGATTTATTCAAGAAGATTAGGAATGATGATTATATGTACTCGGCAGTGATTGAATGCTATGAGACACTCAGGGATATTATATTTGGCCTTCTTGATGATGAAGCTGATAAGATGATTGTAAGGCAGATATGTTATGAAGTAGACAGTAGTATACAGCAGCAGAGGTTTTTGATTAATTTCCGTATGAGTGGGCTGCCATTTCTTAGTGAGAGGTTGGAGAAGTTTCTCAAACTCTTGCTAGCCGAAGATGAGAATGTCGAAAATTCCTTGCGTCAGATAATCAATGTCCTTCAAGATATCATGGAGATTATAACACAGGATGTAATGATGAATGGCCATGAAATCCTCGAAGCAGCTCACTACATAGAGGGTCAAAATGTCAAGAAGGAGCAAAGGCTTCATTTGCTTTTGACCGTCAAGGAATCTGCCATCAATGTGCCCCAAAACTTGGATGCTCGCAGGCGCATTACTTTCTTTGCGAACTCCTTATTCATGAACATGCCAAATGCTCCCAAAGTTCGTGACATGCTCTCCTTTAGTGTTCTGACTCCTTATTACAAAGAAGATGTTCTTTATTCTGATGAGGAACTTACCAAGGAAAATGAGGATGGAATCTCAATTTTGTTCTACCTGCAGAAGATATATCCTGATGAATGGACAAATTTCCTGGACCGCATAAATGAACATAAAAGTCAGTACACTGAAAAGGACAAGTCGGAGTTAATTCGTCAGTGGGTATCTTACAGGGGACAGACACTTTCTAGAACAGTGAGAGGGATGATGTACTATAGGAAGGCTCTAGACCTTCAATGTATTCTGGAAACAGCAGGAGATGGTGGTGTTTATGGAGGCGACGCAGCCCAGATTTTAAGTGAAAAGGATGAGAAGGATATTCTTGATCGGGCACAAGCTCTGGCAGATTTGAAGTTCACTTATGTAGTTTCTTGTCAGGTCTATGGTGAACAGAAGAATTCCACTGAAGCCCGAGACAAAAGTTGTTACACTAATATTCTCAAGCTGATGTTAACGTATCCATCTCTACGTGTTGCTTACATAGATACAAGAGAGGAGACAGTGAATGGAAAATCCCAAAAGATTCATTTCTCTGTTCTGGTCAAGGGAGGTGACAAATGGGATGAGGAAATATACCGCATCAAGCTTCCAGGTCCTCCAACAGTAATTGGTGAAGGAAAACCTGAAAATCAAAACCACGCCATCGTATTTACTCGTGGAGAAGCCCTGCAAACCATTGACATGAATCAGTGTTTCATCACTCGCTTGGTTCATGTCCAATCAAGAGACAAGTTTTGTGACTATAGGCCAACGAATTTTGGCAAATCCGCTGAGAATCTTTCACATAACAAGGGGCGGCATAAGCAAAGCATCAAAAGATATAATTCTGTTCTGCGTGGGGGATTTATAACACATCATGAGTATATCCAAGTAGGCAAGGGGCGTGATGTTGGAATGAATCAAATATCGCTTTTCGAGGCTAAAGTTGCAAATGGAAATGGAGAGCAGACACTTAGCCGTGATGTTTATAGGCTTGGGCGTCGGTTTGATTTCTATAGAATGCTGTCTTTTTACTTCACAACAGTTGGGTTCTATTTTAGTAGTATGGTAACTGTGCTGACTGTCTATGTGTTTTTATATGGACGTCTATATATGGTTATGAGTGGATTGGAAAGTGAGATCCTGGATAGCCCAGCCATACATCAGACCAAGGCTTTTGAACAAGCTCTGGCTACACAGTCTGTCTTTCAACTGGGCTTGTTGCTGGTCCTTCCCATGGTTATGGAAATTGGTCTGGAGAAAGGATTTCGCACTGCTTTGGGTGATTTCATCATCATGCAGCTGCAGCTGGCCTCTGTTTTCTTCACTTTCCAACTAGGAACAAAAGCACATTACTACGGAAGGACAATCTTACATGGAGGATCTAAATATCGAGCAACTGGCCGTGGTTTTGTTGTTTTTCATGCAAAGTTTGCCGATAACTACAGGCTGTACTCACGAAGTCACTTTGTGAAAGGGTTGGAGCTGTTGATTCTGCTGATTGTGTATGGAGTCTATGGGAGTGCATATAGGAGTTCAAATCTGTATTTTTTCATCACGTTCTCCATGTGGTTTCTGGTTGCTTCCTGGTTGTTTGCTCCGTTTATCTTTAATCCGTCCAGCTTTGACTGGCAAAAAACTGTAGATGATTGGACGGATTGGAAGAGGTGGATGGGAAATCGTGGTGGTATTGGGATCTCACCTGACAAAAGTTGGGAATCATGGTGGGATGAAGAACAGGAACACCTCAAGCACACTATTATCCGTGGAAGAATTATTGAGATGATTCTTGCATGTCGCTTCTTTTTTTACCAATATGGGATTGTTTACCACCTCGATATAGCTCATTTCAGCAAGAGTTTACTGGTTTATGGACTTTCTTGGGTGGTTATGGTAACGGTGCTTCTCGTCTTAAAGATGGTGTCAATGGGTAGACGAAGATTTGGCACTGACTTTCAGCTCATGTTCAGAATTCTGAAGGCGCTTCTTTTTCTTGGCTTCATGTCAGTCATGACTGTTCTATTTGTAGTTTGTGGCCTTACTATTTCAGATCTATTTGCTGCCATTCTTGCCTTCTTGCCCACAGGGTGGGCACTACTTCTTATCGGGCAAGTATGCAGGCCAATGGTTAAGGGAGTAGGATTCTGGGAATCAATTAAGGAGCTGGGAAGAGCCTATGATTACATAATGGGAGTGGTAGTCTTCATGCCGGTTGCCATTTTGTCATGGTTCCCATTTGTCTCAGAGTTCCAAACACGGTTGCTCTTCAACCAAGCATTCAGTCGAGGCCTACAAATTTCAATGATTCTTGCCGGGAGGAAAGATAAGACAGCTTCTAACAGATAG

Protein Analysis

1812

Amino Acids

209.68

Weight (kDa)

8.79

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 50 - 190 1.3e-10 Vta1 like
FKS1_dom1 PF14288 336 - 447 1.3e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 823 - 967 6.7e-57 Callose synthase 1 domain
Glucan_synthase PF02364 993 - 1084 1.1e-29 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1085 - 1268 2.8e-46 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1313 - 1625 9.4e-90 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 1205, 1842, 2372, 2852, 3962
AasI GACNNNNNNGTC 1 cut(s) 858
AatII GACGTC 1 cut(s) 4201
Acc36I ACCTGC 2 cut(s) 502, 3180
Acc65I GGTACC 1 cut(s) 490
AccB1I GGYRCC 1 cut(s) 490
AccB7I CCANNNNNTGG 3 cut(s) 1099, 2989, 5181
AccI GTMKAC 4 cut(s) 716, 2682, 2692, 5026
AclWI GGATC 6 cut(s) 245, 258, 2424, 4228, 4483, 4796
AcoI YGGCCR 5 cut(s) 35, 438, 1337, 2875, 4495
AcuI CTGAAG 8 cut(s) 552, 1570, 1786, 1814, 2131, 2229, 3540, 5090
AcvI CACGTG 1 cut(s) 1175
AcyI GRCGYC 2 cut(s) 4093, 4198
AdeI CACNNNGTG 3 cut(s) 2311, 4564, 5181
AfaI GTAC 8 cut(s) 465, 492, 897, 1229, 2579, 3239, 3326, 4547
AfeI AGCGCT 1 cut(s) 639
AfiI CCNNNNNNNGG 7 cut(s) 33, 101, 1099, 2158, 2989, 4330, 5181
AflIII ACRYGT 2 cut(s) 1613, 3583
AjiI CACGTC 1 cut(s) 269
AjnI CCWGG 7 cut(s) 440, 1218, 2073, 3207, 3703, 4236, 4679
AjuI GAANNNNNNNTTGG 2 cut(s) 2291, 2323
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
Alw21I GWGCWC 3 cut(s) 11, 480, 649
Alw26I GTCTC 6 cut(s) 2197, 2597, 3522, 3605, 3834, 5338
AlwI GGATC 6 cut(s) 245, 258, 2424, 4228, 4483, 4796
AlwNI CAGNNNCTG 1 cut(s) 1727
Ama87I CYCGRG 1 cut(s) 3525
Aor51HI AGCGCT 1 cut(s) 639
ArsI GACNNNNNNTTYG 4 cut(s) 258, 280, 290, 312
AseI ATTAAT 1 cut(s) 2715
Asp700I GAANNNNTTC 2 cut(s) 1799, 3108
Asp718I GGTACC 1 cut(s) 490
AspA2I CCTAGG 1 cut(s) 197
AspLEI GCGC 5 cut(s) 241, 640, 1941, 3006, 5077
AspS9I GGNCC 6 cut(s) 18, 1401, 2223, 3211, 3707, 4321
AsuC2I CCSGG 2 cut(s) 167, 5407
AsuHPI GGTGA 6 cut(s) 1055, 3512, 3677, 3737, 4388, 4785
AsuII TTCGAA 1 cut(s) 2329
AsuNHI GCTAGC 2 cut(s) 2483, 2779
AvaI CYCGRG 1 cut(s) 3525
AvaII GGWCC 6 cut(s) 18, 1401, 2223, 3211, 3707, 4321
AvrII CCTAGG 1 cut(s) 197
BaeGI GKGCMC 3 cut(s) 2982, 3448, 5188
BaeI ACNNNNGTAYC 2 cut(s) 474, 507
BalI TGGCCA 3 cut(s) 440, 1339, 2877
BamHI GGATCC 1 cut(s) 250
BanI GGYRCC 1 cut(s) 490
BanII GRGCYC 1 cut(s) 11
BarI GAAGNNNNNNTAC 2 cut(s) 4980, 5012
BauI CACGAG 1 cut(s) 3768
BbrPI CACGTG 1 cut(s) 1175
BbsI GAAGAC 4 cut(s) 1576, 1921, 2397, 5287
Bbv12I GWGCWC 3 cut(s) 11, 480, 649
BceAI ACGGC 3 cut(s) 118, 4056, 4482
BciT130I CCWGG 7 cut(s) 442, 1220, 2075, 3209, 3705, 4238, 4681
BciVI GTATCC 2 cut(s) 1374, 3582
BclI TGATCA 1 cut(s) 2397
BcnI CCSGG 2 cut(s) 167, 5407
BcoDI GTCTC 6 cut(s) 2197, 2597, 3522, 3605, 3834, 5338
BfaI CTAG 9 cut(s) 78, 198, 1331, 1764, 2484, 2780, 3303, 3341, 4430
BfoI RGCGCY 2 cut(s) 641, 5078
BfuAI ACCTGC 2 cut(s) 502, 3180
BfuI GTATCC 2 cut(s) 1374, 3582
BglII AGATCT 1 cut(s) 5143
BlnI CCTAGG 1 cut(s) 197
Bme1390I CCNGG 9 cut(s) 167, 442, 1220, 2075, 3209, 3705, 4238, 4681, 5407
Bme18I GGWCC 6 cut(s) 18, 1401, 2223, 3211, 3707, 4321
BmeT110I CYCGRG 1 cut(s) 3525
BmgBI CACGTC 1 cut(s) 269
BmgT120I GGNCC 6 cut(s) 18, 1401, 2223, 3211, 3707, 4321
BmiI GGNNCC 7 cut(s) 19, 252, 492, 974, 1869, 2143, 5324
BmrFI CCNGG 9 cut(s) 167, 442, 1220, 2075, 3209, 3705, 4238, 4681, 5407
BmrI ACTGGG 1 cut(s) 4316
BmsI GCATC 9 cut(s) 100, 1336, 1564, 1611, 2080, 2278, 2983, 3702, 3933
BmtI GCTAGC 2 cut(s) 2487, 2783
BmuI ACTGGG 1 cut(s) 4316
BpiI GAAGAC 4 cut(s) 1576, 1921, 2397, 5287
BpmI CTGGAG 2 cut(s) 1572, 4370
Bpu14I TTCGAA 1 cut(s) 2329
BpuEI CTTGAG 3 cut(s) 464, 3539, 4826
BpuMI CCSGG 2 cut(s) 167, 5407
BsaAI YACGTR 2 cut(s) 1175, 3584
BsaBI GATNNNNATC 1 cut(s) 2415
BsaHI GRCGYC 2 cut(s) 4093, 4198
BsaJI CCNNGG 9 cut(s) 21, 197, 223, 246, 3135, 4263, 4329, 4498, 4858
BsaWI WCCGGW 1 cut(s) 427
BsaXI ACNNNNNCTCC 6 cut(s) 2153, 2183, 3056, 3086, 5277, 5307
Bsc4I CCNNNNNNNGG 7 cut(s) 33, 101, 1099, 2158, 2989, 4330, 5181
Bse118I RCCGGY 1 cut(s) 5302
Bse1I ACTGG 7 cut(s) 1388, 1420, 1940, 4311, 4498, 4727, 4962
Bse3DI GCAATG 4 cut(s) 1017, 1138, 2049, 2337
Bse8I GATNNNNATC 1 cut(s) 2415
BseBI CCWGG 7 cut(s) 442, 1220, 2075, 3209, 3705, 4238, 4681
BseDI CCNNGG 9 cut(s) 21, 197, 223, 246, 3135, 4263, 4329, 4498, 4858
BseJI GATNNNNATC 1 cut(s) 2415
BseLI CCNNNNNNNGG 7 cut(s) 33, 101, 1099, 2158, 2989, 4330, 5181
BseMI GCAATG 4 cut(s) 1017, 1138, 2049, 2337
BseMII CTCAG 8 cut(s) 615, 855, 1672, 2210, 2366, 2623, 3876, 5349
BseNI ACTGG 7 cut(s) 1388, 1420, 1940, 4311, 4498, 4727, 4962
BseRI GAGGAG 1 cut(s) 3623
BseSI GKGCMC 3 cut(s) 2982, 3448, 5188
BseYI CCCAGC 2 cut(s) 4246, 5257
BshNI GGYRCC 1 cut(s) 490
BsiHKAI GWGCWC 3 cut(s) 11, 480, 649
BsiHKCI CYCGRG 1 cut(s) 3525
BsiSI CCGG 4 cut(s) 166, 428, 5303, 5406
BslFI GGGAC 3 cut(s) 31, 329, 3303
BslI CCNNNNNNNGG 7 cut(s) 33, 101, 1099, 2158, 2989, 4330, 5181
BsmAI GTCTC 6 cut(s) 2197, 2597, 3522, 3605, 3834, 5338
BsmFI GGGAC 3 cut(s) 31, 329, 3303
BsmI GAATGC 8 cut(s) 687, 1079, 1369, 1672, 2029, 2600, 4119, 5369
BsoBI CYCGRG 1 cut(s) 3525
Bsp119I TTCGAA 1 cut(s) 2329
Bsp1286I GDGCHC 6 cut(s) 11, 480, 649, 2982, 3448, 5188
Bsp1407I TGTACA 2 cut(s) 895, 1227
Bsp19I CCATGG 3 cut(s) 223, 246, 4329
BspCNI CTCAG 8 cut(s) 616, 856, 1671, 2209, 2365, 2622, 3877, 5348
BspHI TCATGA 4 cut(s) 1608, 3033, 3970, 5103
BspLI GGNNCC 7 cut(s) 19, 252, 492, 974, 1869, 2143, 5324
BspMAI CTGCAG 2 cut(s) 3177, 4401
BspMI ACCTGC 2 cut(s) 502, 3180
BspOI GCTAGC 2 cut(s) 2487, 2783
BspPI GGATC 6 cut(s) 245, 258, 2424, 4228, 4483, 4796
BspQI GCTCTTC 3 cut(s) 1670, 5257, 5363
BspT104I TTCGAA 1 cut(s) 2329
BspT107I GGYRCC 1 cut(s) 490
BsrDI GCAATG 4 cut(s) 1017, 1138, 2049, 2337
BsrFI RCCGGY 1 cut(s) 5302
BsrGI TGTACA 2 cut(s) 895, 1227
BsrI ACTGG 7 cut(s) 1388, 1420, 1940, 4311, 4498, 4727, 4962
BssAI RCCGGY 1 cut(s) 5302
BssECI CCNNGG 9 cut(s) 21, 197, 223, 246, 3135, 4263, 4329, 4498, 4858
BssNAI GTATAC 1 cut(s) 2693
BssNI GRCGYC 2 cut(s) 4093, 4198
BssSI CACGAG 1 cut(s) 3768
BssT1I CCWWGG 7 cut(s) 21, 197, 223, 246, 3135, 4263, 4329
Bst1107I GTATAC 1 cut(s) 2693
Bst2BI CACGAG 1 cut(s) 3768
Bst2UI CCWGG 7 cut(s) 442, 1220, 2075, 3209, 3705, 4238, 4681
Bst6I CTCTTC 5 cut(s) 426, 1670, 4751, 5257, 5363
BstACI GRCGYC 2 cut(s) 4093, 4198
BstAPI GCANNNNNTGC 1 cut(s) 4526
BstAUI TGTACA 2 cut(s) 895, 1227
BstBAI YACGTR 2 cut(s) 1175, 3584
BstBI TTCGAA 1 cut(s) 2329
BstC8I GCNNGC 9 cut(s) 7, 342, 2481, 2485, 2781, 2998, 3002, 4404, 5213
BstDSI CCRYGG 5 cut(s) 223, 246, 4329, 4498, 4858
BstH2I RGCGCY 2 cut(s) 641, 5078
BstHHI GCGC 5 cut(s) 241, 640, 1941, 3006, 5077
BstMAI GTCTC 6 cut(s) 2197, 2597, 3522, 3605, 3834, 5338
BstNI CCWGG 7 cut(s) 442, 1220, 2075, 3209, 3705, 4238, 4681
BstNSI RCATGY 4 cut(s) 1617, 3043, 3070, 4890
BstSCI CCNGG 9 cut(s) 165, 440, 1218, 2073, 3207, 3703, 4236, 4679, 5405
BstSLI GKGCMC 3 cut(s) 2982, 3448, 5188
BstV2I GAAGAC 4 cut(s) 1576, 1921, 2397, 5287
BstX2I RGATCY 6 cut(s) 250, 2416, 4233, 4475, 4788, 5143
BstXI CCANNNNNNTGG 1 cut(s) 4336
BstYI RGATCY 6 cut(s) 250, 2416, 4233, 4475, 4788, 5143
BstZ17I GTATAC 1 cut(s) 2693
BsuI GTATCC 2 cut(s) 1374, 3582
BtgI CCRYGG 5 cut(s) 223, 246, 4329, 4498, 4858
BtrI CACGTC 1 cut(s) 269
BtsI GCAGTG 2 cut(s) 2592, 4365
BveI ACCTGC 2 cut(s) 502, 3180
Cac8I GCNNGC 9 cut(s) 7, 342, 2481, 2485, 2781, 2998, 3002, 4404, 5213
CaiI CAGNNNCTG 1 cut(s) 1727
CciI TCATGA 4 cut(s) 1608, 3033, 3970, 5103
CfoI GCGC 5 cut(s) 241, 640, 1941, 3006, 5077
Cfr10I RCCGGY 1 cut(s) 5302
Cfr13I GGNCC 6 cut(s) 18, 1401, 2223, 3211, 3707, 4321
CseI GACGC 4 cut(s) 135, 2801, 3404, 4082
CsiI ACCWGGT 1 cut(s) 1218
Csp6I GTAC 8 cut(s) 464, 491, 896, 1228, 2578, 3238, 3325, 4546
CviQI GTAC 8 cut(s) 464, 491, 896, 1228, 2578, 3238, 3325, 4546
DraI TTTAAA 2 cut(s) 691, 1125
DraIII CACNNNGTG 3 cut(s) 2311, 4564, 5181
DrdI GACNNNNNNGTC 1 cut(s) 858
DseDI GACNNNNNNGTC 1 cut(s) 858
EaeI YGGCCR 5 cut(s) 35, 438, 1337, 2875, 4495
Eam1104I CTCTTC 5 cut(s) 426, 1670, 4751, 5257, 5363
EarI CTCTTC 5 cut(s) 426, 1670, 4751, 5257, 5363
EciI GGCGGA 1 cut(s) 1840
Ecl136II GAGCTC 1 cut(s) 9
Eco130I CCWWGG 7 cut(s) 21, 197, 223, 246, 3135, 4263, 4329
Eco147I AGGCCT 1 cut(s) 5382
Eco24I GRGCYC 1 cut(s) 11
Eco32I GATATC 1 cut(s) 2839
Eco47I GGWCC 6 cut(s) 18, 1401, 2223, 3211, 3707, 4321
Eco47III AGCGCT 1 cut(s) 639
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 8 cut(s) 552, 1570, 1786, 1814, 2131, 2229, 3540, 5090
Eco72I CACGTG 1 cut(s) 1175
Eco88I CYCGRG 1 cut(s) 3525
EcoICRI GAGCTC 1 cut(s) 9
EcoO109I RGGNCCY 1 cut(s) 3707
EcoRI GAATTC 2 cut(s) 3511, 5064
EcoRII CCWGG 7 cut(s) 440, 1218, 2073, 3207, 3703, 4236, 4679
EcoRV GATATC 1 cut(s) 2839
EcoT14I CCWWGG 7 cut(s) 21, 197, 223, 246, 3135, 4263, 4329
EcoT22I ATGCAT 2 cut(s) 687, 1672
EcoT38I GRGCYC 1 cut(s) 11
ErhI CCWWGG 7 cut(s) 21, 197, 223, 246, 3135, 4263, 4329
FalI AAGNNNNNCTT 4 cut(s) 2291, 2323, 3096, 3128
FaqI GGGAC 3 cut(s) 31, 329, 3303
FauI CCCGC 1 cut(s) 8
FauNDI CATATG 1 cut(s) 1037
FbaI TGATCA 1 cut(s) 2397
FblI GTMKAC 4 cut(s) 716, 2682, 2692, 5026
FriOI GRGCYC 1 cut(s) 11
FspBI CTAG 9 cut(s) 78, 198, 1331, 1764, 2484, 2780, 3303, 3341, 4430
GlaI GCGC 5 cut(s) 240, 639, 1940, 3005, 5076
GsaI CCCAGC 2 cut(s) 4250, 5261
GsuI CTGGAG 2 cut(s) 1572, 4370
HaeII RGCGCY 2 cut(s) 641, 5078
HapII CCGG 4 cut(s) 166, 428, 5303, 5406
HgaI GACGC 4 cut(s) 135, 2801, 3404, 4082
HhaI GCGC 5 cut(s) 241, 640, 1941, 3006, 5077
Hin1I GRCGYC 2 cut(s) 4093, 4198
Hin6I GCGC 5 cut(s) 239, 638, 1939, 3004, 5075
HinP1I GCGC 5 cut(s) 239, 638, 1939, 3004, 5075
HincII GTYRAC 2 cut(s) 717, 3567
HindII GTYRAC 2 cut(s) 717, 3567
HindIII AAGCTT 2 cut(s) 1047, 3698
HpaI GTTAAC 1 cut(s) 3567
HpaII CCGG 4 cut(s) 166, 428, 5303, 5406
HphI GGTGA 6 cut(s) 1055, 3512, 3677, 3737, 4388, 4785
Hpy99I CGWCG 5 cut(s) 100, 110, 139, 3398, 4098
HpyCH4IV ACGT 6 cut(s) 268, 1174, 3569, 3583, 4198, 4652
HpySE526I ACGT 6 cut(s) 268, 1174, 3569, 3583, 4198, 4652
Hsp92I GRCGYC 2 cut(s) 4093, 4198
HspAI GCGC 5 cut(s) 239, 638, 1939, 3004, 5075
KpnI GGTACC 1 cut(s) 494
Ksp22I TGATCA 1 cut(s) 2397
KspAI GTTAAC 1 cut(s) 3567
LguI GCTCTTC 3 cut(s) 1670, 5257, 5363
LweI GCATC 9 cut(s) 100, 1336, 1564, 1611, 2080, 2278, 2983, 3702, 3933
MabI ACCWGGT 1 cut(s) 1218
MaeI CTAG 9 cut(s) 78, 198, 1331, 1764, 2484, 2780, 3303, 3341, 4430
MaeII ACGT 6 cut(s) 268, 1174, 3569, 3583, 4198, 4652
MaeIII GTNAC 8 cut(s) 1687, 3062, 3539, 3665, 3851, 4162, 4556, 4987
MfeI CAATTG 1 cut(s) 1001
MflI RGATCY 6 cut(s) 250, 2416, 4233, 4475, 4788, 5143
MhlI GDGCHC 6 cut(s) 11, 480, 649, 2982, 3448, 5188
MlsI TGGCCA 3 cut(s) 440, 1339, 2877
MluNI TGGCCA 3 cut(s) 440, 1339, 2877
MlyI GAGTC 6 cut(s) 1552, 1822, 2056, 2243, 3082, 4614
Mox20I TGGCCA 3 cut(s) 440, 1339, 2877
Mph1103I ATGCAT 2 cut(s) 687, 1672
MroXI GAANNNNTTC 2 cut(s) 1799, 3108
MscI TGGCCA 3 cut(s) 440, 1339, 2877
MslI CAYNNNNRTG 8 cut(s) 1374, 1607, 2098, 2975, 3038, 3794, 4334, 4388
Msp20I TGGCCA 3 cut(s) 440, 1339, 2877
MspA1I CMGCKG 4 cut(s) 15, 3884, 4396, 4402
MspI CCGG 4 cut(s) 166, 428, 5303, 5406
MspR9I CCNGG 9 cut(s) 167, 442, 1220, 2075, 3209, 3705, 4238, 4681, 5407
MunI CAATTG 1 cut(s) 1001
Mva1269I GAATGC 8 cut(s) 687, 1079, 1369, 1672, 2029, 2600, 4119, 5369
MvaI CCWGG 7 cut(s) 442, 1220, 2075, 3209, 3705, 4238, 4681
NciI CCSGG 2 cut(s) 167, 5407
NcoI CCATGG 3 cut(s) 223, 246, 4329
NdeI CATATG 1 cut(s) 1037
NheI GCTAGC 2 cut(s) 2483, 2779
NlaIV GGNNCC 7 cut(s) 19, 252, 492, 974, 1869, 2143, 5324
NmeAIII GCCGAG 1 cut(s) 2561
NmuCI GTSAC 5 cut(s) 1687, 3062, 3665, 3851, 4556
NsiI ATGCAT 2 cut(s) 687, 1672
NspI RCATGY 4 cut(s) 1617, 3043, 3070, 4890
NspV TTCGAA 1 cut(s) 2329
PagI TCATGA 4 cut(s) 1608, 3033, 3970, 5103
PceI AGGCCT 1 cut(s) 5382
PciI ACATGT 1 cut(s) 1613
PciSI GCTCTTC 3 cut(s) 1670, 5257, 5363
PcsI WCGNNNNNNNCGW 2 cut(s) 143, 274
PctI GAATGC 8 cut(s) 687, 1079, 1369, 1672, 2029, 2600, 4119, 5369
PdmI GAANNNNTTC 2 cut(s) 1799, 3108
PflMI CCANNNNNTGG 3 cut(s) 1099, 2989, 5181
PfoI TCCNGGA 2 cut(s) 3207, 4236
PleI GAGTC 6 cut(s) 1551, 1821, 2056, 2242, 3082, 4613
PmaCI CACGTG 1 cut(s) 1175
PmlI CACGTG 1 cut(s) 1175
PpsI GAGTC 6 cut(s) 1551, 1821, 2056, 2242, 3082, 4613
Ppu21I YACGTR 2 cut(s) 1175, 3584
PpuMI RGGWCCY 1 cut(s) 3707
PscI ACATGT 1 cut(s) 1613
PshBI ATTAAT 1 cut(s) 2715
PsiI TTATAA 5 cut(s) 1205, 1842, 2372, 2852, 3962
Psp124BI GAGCTC 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 3707
Psp6I CCWGG 7 cut(s) 440, 1218, 2073, 3207, 3703, 4236, 4679
PspCI CACGTG 1 cut(s) 1175
PspFI CCCAGC 2 cut(s) 4246, 5257
PspGI CCWGG 7 cut(s) 440, 1218, 2073, 3207, 3703, 4236, 4679
PspN4I GGNNCC 7 cut(s) 19, 252, 492, 974, 1869, 2143, 5324
PspPI GGNCC 6 cut(s) 18, 1401, 2223, 3211, 3707, 4321
PspPPI RGGWCCY 1 cut(s) 3707
PstI CTGCAG 2 cut(s) 3177, 4401
PstNI CAGNNNCTG 1 cut(s) 1727
PsuI RGATCY 6 cut(s) 250, 2416, 4233, 4475, 4788, 5143
PvuII CAGCTG 2 cut(s) 4396, 4402
RsaI GTAC 8 cut(s) 465, 492, 897, 1229, 2579, 3239, 3326, 4547
RsaNI GTAC 8 cut(s) 464, 491, 896, 1228, 2578, 3238, 3325, 4546
RseI CAYNNNNRTG 8 cut(s) 1374, 1607, 2098, 2975, 3038, 3794, 4334, 4388
SacI GAGCTC 1 cut(s) 11
SalI GTCGAC 1 cut(s) 715
SapI GCTCTTC 3 cut(s) 1670, 5257, 5363
Sau96I GGNCC 6 cut(s) 18, 1401, 2223, 3211, 3707, 4321
SchI GAGTC 6 cut(s) 1552, 1822, 2056, 2243, 3082, 4614
ScrFI CCNGG 9 cut(s) 167, 442, 1220, 2075, 3209, 3705, 4238, 4681, 5407
SduI GDGCHC 6 cut(s) 11, 480, 649, 2982, 3448, 5188
SexAI ACCWGGT 1 cut(s) 1218
SfaNI GCATC 9 cut(s) 100, 1336, 1564, 1611, 2080, 2278, 2983, 3702, 3933
SfuI TTCGAA 1 cut(s) 2329
SinI GGWCC 6 cut(s) 18, 1401, 2223, 3211, 3707, 4321
SmiMI CAYNNNNRTG 8 cut(s) 1374, 1607, 2098, 2975, 3038, 3794, 4334, 4388
SmlI CTYRAG 3 cut(s) 479, 3554, 4841
SmoI CTYRAG 3 cut(s) 479, 3554, 4841
SseBI AGGCCT 1 cut(s) 5382
SspI AATATT 5 cut(s) 1569, 1698, 2038, 2112, 3550
SspMI CTAG 9 cut(s) 78, 198, 1331, 1764, 2484, 2780, 3303, 3341, 4430
SstI GAGCTC 1 cut(s) 11
StuI AGGCCT 1 cut(s) 5382
StyD4I CCNGG 9 cut(s) 165, 440, 1218, 2073, 3207, 3703, 4236, 4679, 5405
StyI CCWWGG 7 cut(s) 21, 197, 223, 246, 3135, 4263, 4329
TaiI ACGT 6 cut(s) 271, 1177, 3572, 3586, 4201, 4655
TatI WGTACW 7 cut(s) 463, 895, 1227, 2577, 3237, 3324, 4545
TauI GCSGC 4 cut(s) 40, 219, 1954, 3913
TseFI GTSAC 5 cut(s) 1687, 3062, 3665, 3851, 4556
Tsp45I GTSAC 5 cut(s) 1687, 3062, 3665, 3851, 4556
Van91I CCANNNNNTGG 3 cut(s) 1099, 2989, 5181
VpaK11BI GGWCC 6 cut(s) 18, 1401, 2223, 3211, 3707, 4321
VspI ATTAAT 1 cut(s) 2715
XbaI TCTAGA 2 cut(s) 3302, 3340
XceI RCATGY 4 cut(s) 1617, 3043, 3070, 4890
XcmI CCANNNNNNNNNTGG 1 cut(s) 4666
XmaJI CCTAGG 1 cut(s) 197
XmiI GTMKAC 4 cut(s) 716, 2682, 2692, 5026
XmnI GAANNNNTTC 2 cut(s) 1799, 3108
XspI CTAG 9 cut(s) 78, 198, 1331, 1764, 2484, 2780, 3303, 3341, 4430
ZraI GACGTC 1 cut(s) 4199
Zsp2I ATGCAT 2 cut(s) 687, 1672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.