Rh2CG475800

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0009173
Physical Location & Seq
Reverse (-)
64099417 .. 64115750
16334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG475800.1

Sequence Viewer

Length: 5793 bp
ATGGCGAGCTCCAGCGGGACCAAGGCCGATTCGCGGCCGCAGAGGTCGCTGTCGAGGGGAATGACGAGAATGGCTACTAGAGTTGTGGACTTTCCGACGGAGGGCGACGATGCGTTGGACAGTGGAGTTGTGCCGTCGTGCCTTGCGTCTATTGCTCCGATTTTCCGGGTTGCCAATGAGATTGAGAAGGACAACCCTAGGGTTGCTTATCTCTGCCGCTTCCATGGATTTGAAAAGGCACATACCATGGATCCAACATCAAGTGGACGTGGAGTTCGTCAGTTTAAGACGCATCTTTTGCACAGACTCGAAAGGGACGAAGTAGATACAAAACATCAGCTTGCTAAATCTGATACAAAAGAAATCATGTTGTATTACCACCAATTCTATGAGAAAAATATCAGAGATGGAGAATATACAAAGAAACCGGAAGAGATGGCCAGGATTTGTCAGATTGCAACTGTACTGTATGATGTGCTCAAGACAGTGGTACCTGCCTCTCAAATAGACATACAGACCCAGAGAATTGCTGAAGATGTCAAGAGCAAAAGGGAGCAATATGTAAACTATAACATTCTTCCATTATATACTGTCGGGGTTAAACCGACAATTATGGAACTTCCTGAGATCAAAGCAGCGCTCCGTGCTCTACAGAATGTGAGTAATCTTCCAATGCCAAGAATGCATTTTAAACCCACGAATCCTGATGACAAGTCGACAATGCCTACAGAGAGGATTAAACCTGTGAATGACATACTCGATTGGCTTTCGGCTATTTTTGGGTTTCAGAAAGGAAATGTGGCAAATCAGAGGGAACATTTAATTTTGCTGCTTGCCAACATTGATGTAAGACATAGAAGTCCTGAGAACTACGATGAGTTGAATAGTGGCACTGTACAGCATTTGATGGAGAAATTCTTTAAGAATTATCGTTCATGGTATAACTATCTGCATTGTAAAACAAATCTTCGGTTCCCCGAAGGTTCTCACAGCAAACAACAATTGGAACTTATATACATTGCTCTCTATCTTCTCATATGGGGTGAAGCTTCAAATGTCCGATTCATGCCTGAATGCTTATGCTACATTTTCCATAATATGGCGAACGAGGTCTATGGAATTTTAAATAGCAATGCTCATACTGTTAGTGGAGAAACATACCAAACAGTTGTACGTGATGAAGAATCTTTTTTGAGGGATGTTATAACTCCTATCTACCAGGTTTTGTACAAGGAAGCAAAGAGAAACAAAATGGGCAAGGCAAGTCATTCAAGATGGAGAAATTATGATGATTTGAATGAATACTTCTGGTCGGACAAATGTTTTAAGCTAGGGTGGCCAATGGATGCTAAAGCAGATTTCTTTAGGCATTCAGATGGGATACAACCAGTAAATGAGAGGACCAATCAAGCTACTGGTGGTAGGAGGAAGCCTAAAACAAATTTTGTAGAAGTCCGAACATTTTTACACCTCTACAGAAGTTTTGATCGAATGTGGATATTCTTCATATTGGCTTTACAGGCTATGATTATTATTGCATGGAGTCCTTCTGGATCTATCACTTCAATATTTGATGCAGACGTCTTCAGAAGTGTTTTAAGCATCTTCATGACATATGCTTTCCTTAATTTATTACAAGCCACTTTGGACATAATTCTCAGTTGGAATGCATGGAAGAGCTTGAAAGTCACTCAAATATTACGATACCTCTTGAAATTTGCAGTAGCTGTTGTCTGGGCTGTGGTTCTGCCTATTGGTTATTCTAGTTCTATGCAAAATCCTACAGGGCTTCTGAAATTCTTCAGTAGTTGGAGTCGGGATTGGCGGAATCAGTCATTTTATAATTATGCTGTTGCAATCTATATGGTTCCTAATTTATTGGCTGCTATGCTATTTTTTCTTCCACCTTTACGAAGACACATAGAGCGTTCCAACTGGCGCATTGTCACTCTTTTTATGTGGTGGGCTCAGCCAAAACTGTATGTCGGAAGAGGAATGCATGAGGATGTCTTCTCACTGCTGAAATATACATTGTTCTGGATCATGCTGCTAATTAGCAAGCTAGCGTTCAGCTATTATGTGGAGATATTGCCGCTTGTTGCACCAACAAAGTTGATAATGGATATGCCTATCAGTAATTACCAATGGCATGAGTTCTTTCCGAATGCAACTCATAATATTGGTGTTGTCATTGCAATATGGACTCCAATTATCCTGGTATATTTTATGGATGCACAAATATGGTATGCAATATTTTCTACTCTGTTTGGTGGGATTCATGGAGCCTTCAGCCATTTGGGGGAGATACGAACACTTGGGATGTTGCGGTCCAGATTTGAGTCTGTGCCTTCAGCTTTCAGTGATCGGCTTATGCCATCGTCAGAGAATAAGGATGCCAGAAAGAAGAAACACTTGGTGGATGAAGGACTTGTTCGAAAGAACATTGCTAATTTCTCTCAGGTCTGGAATGAATTTATAAATTCTATGCGAATGGAAGACCTGATCAGCAATAGGGATAGGGATCTGCTGCTTGTTCCGTATTCTTCAAGTGATGTTTCTGTTGTCCAGTGGCCTCCTTTCTTGCTTGCTAGCAAGATTCCAATAGCATTAGACATGGCAAAGGATTTTACAGGAAAGTCGGATGGTGATTTATTCAGGAAGATTAAGAATGATGATTATATGTACTCGGCAGTGATTGAATGCTATGAGACACTCAGGGATATTATATTTGGCCTTCTTGATGATGACGCCGATAAGATGATTGTAAGGCAGATATGTTATGAAGTAGACAGTAGTATACAGCAGCAGAGGTTTTTGAATAATTTCCGTATGAGTGGGCTGCCATTTCTTAGTGAGAGGTTGGAGAAGTTTCTCAAACTCTTGCTAGCCGAAGATGAGAATGTCGAAAATTCCTTGCGTCAGATAATCAATGTCCTTCAAGATATCATGGAGATTATAACACAGGATGTAATGATCAATGGCCATGAAATCCTCGAAGCAGCTCACTACATAGAGGGTCAAAATGTCAAGAAGGAGCAAAGGTTTCAAAAAATCAACATATATCTTGCCCAAAATCCATCTTGGAGAGAGAAGGTCGTTAGGCTTCATTTGCTTTTGACCGTCAAGGAATCTGCCATCAATGTGCCCCAAAACTTGGATGCTCGCAGGCGCATTACTTTCTTTGCGAACTCCTTATTCATGAACATGCCAAATGCTCCCAAAGTTCGTGACATGCTCTCCTTTAGTGTTCTGACTCCTTATTACAAAGAAGATGTTCTTTATTCTGATGAGGAACTTACCAAGGAAAATGAGGATGGAATCTCAATTTTGTTCTACCTGCAGAAGATATATCCTGATGAATGGACAAATTTCCTGGACCGCATAAATGAACATAAAAGTCAGTACACTGAAAAGGACAAGTCGGAGTTAATTCGTCAGTGGGTATCTTACAGGGGACAGACACTTTCTAGAACAGTGAGAGGGATGATGTACTATAGGAAGGCTCTAGACCTTCAATGTATCCTGGAAACAGCAGGAGATGGTGGTGTTTATGGAGGCGACGCAGCCCATATTTTAAGTGATAAAGATGAGAAGGATATTCTTGATCGGGCACAAGCTCTGGCAGATTTGAAGTTCACTTATGTAGTTTCTTGTCAGGTCTATGGTGAACAGAAGAATTCCACTGAAGCCCGAGACAAAAGTTGTTACACTAATATTCTCAAGCTGATGTTAACGTATCCATCTCTACGTGTTGCTTACATAGATACAAGAGAGAAGACAGTGAATGGAAAATCCCAAAAGATTCATTTCTCTGTTCTGGTCAAGGGAGGTGACAAATGGGATGAGGAAATATACCGCATCAAGCTTCCAGGTCCTCCAACAGCAATTGGTGAAGGAAAACCCGAAAATCAAAACCATGCCATCGTATTTACTCGTGGAGAAGCCCTGCAAACCATTGACATGAATCAGGATAATTATTTTGAGGAAGCTTTCAAAATGAGAAATGTATTGGAGGAATTTCTAAAGCCTCGACGGGGGCAGCGGAAACCTACAATATTGGGTCTAAGGGAACATATATTTACCGGAAGTGTTTCATCACTCGCTTGGTTCATGTCCAATCAAGAGACAAGTTTTGTGACTATAGGACAACGAATTTTGGCAAATCCGCTGAGGGTACGGTTTCATTATGGCCATCCTGATATTTTTGACAGAATCTTTCACATAACAAGAGGCGGCATAAGCAAAGCATCAAAAGTAATTAACTTAAGCGAGGATATATTTTCAGGATATAATTCTGTTCTGCGTGGGGGATTTATAACACATCATGAGTATATCCAAGTAGGCAAGGGGCGTGATGTTGGAATGAATCAAATATCGCTTTTCGAGGCTAAAGTTGCAAATGGAAATGGAGAGCAGACACTTAGCCGTGATGTTTATAGGCTTGGGCGTCGGTTTGATTTCTATAGAATGCTGTCTTTTTACTTCACAACAGTTGGGTTCTATTTTAGTAGTATGGTAACTGTGCTGACTGTCTATGTGTTTTTATACGGACGTCTATATATGGTTATGAGTGGATTGGAAAGTGAGATCCTGGATAGCCCAGCCATACATCAGACCAAGGCTTTTGAACAAGCTCTGGCTACACAGTCTGTCTTTCAACTGGGCTTGTTGCTGGTCCTTCCCATGGTTATGGAAATTGGTCTGGAGAAAGGATTTCGCACTGCCTTGGGTGATTTCATCATCATGCAGCTGCAGCTGGCCTCTGTTTTCTTCACTTTCCAACTAGGAACAAAAGCACATTACTACGGAAGGACAATCTTACATGGAGGATCTAAATATCGAGCAACTGGCCGTGGTTTTGTTGTTTTTCATGCAAAGTTTGCCGATAACTACAGGCTGTACTCACGAAGTCACTTTGTGAAAGGGTTGGAGCTGTTGATTCTGCTGATTGTGTATGGAGTCTATGGGAATGCATATAGGAGTTCAAATCTGTATTTTTTCATCACGTTCTCCATGTGGTTTCTGGTTGCTTCCTGGTTGTTTGCTCCTTTTATCTTTAATCCGTCCAGCTTTGACTGGCAAAAAACTGTAGATGATTGGACGGATTGGAAGAGGTGGATGGGAAATCGTGGTGGTATTGGGATCTCACCTGACAAAAGTTGGGAATCATGGTGGGATGAAGAACAGGAACACCTCAAGCACACTATTATCCGTGGAAGAATTATTGAGATAATTCTTGCATGTCGCTTCTTTTTTTACCAATATGGGATTGTTTACCACCTCGATATAGCTCATTTCAGCAAGAGTTTACTGGTTTATGGACTTTCTTGGGTGGTTATGGTAACGGTGCTCCTCGTCTTAAAGATGGTGTCAATGGGTAGACGAAGATTTGGCACTGACTTTCAGCTCATGTTCAGAATTCTGAAGGCGCTTCTTTTTCTTGGCTTCATGTCAGTCATGACTGTCCTATTTGTAGTTTGTGGCCTTACTATTTCTGATCTATTTGCTGCTATTCTTGCCTTCTTGCCCACAGGGTGGGCACTACTTCTTATCGGGCAAGTATGCAGGCCAATGGTTAAGGGAGTAGGATTCTGGGAATCAATTAAGGAGCTGGGAAGAGCCTATGATTACATAATGGGAGTGGTAGTCTTCATGCCGGTTGCCATTTTGTCATGGTTCCCATTTGTCTCAGAGTTCCAGACACGGTTGCTCTTCAACCAAGCATTCAGTCGAGGCCTACAAATTTCAATGATTCTCGCCGGGAGGAAAGATAAGACAGCTTCTAATAGATAG

Protein Analysis

1930

Amino Acids

223.55

Weight (kDa)

8.96

Isoelectric Point (pI)

39.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 50 - 180 1.9e-10 Vta1 like
FKS1_dom1 PF14288 336 - 447 1.8e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 858 - 1041 3.2e-70 Callose synthase 1 domain
Glucan_synthase PF02364 1048 - 1138 1.4e-29 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1142 - 1742 4.4e-201 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 1205, 1842, 2477, 2957, 4316
AasI GACNNNNNNGTC 1 cut(s) 858
AatII GACGTC 2 cut(s) 1584, 4555
Acc36I ACCTGC 2 cut(s) 502, 3345
Acc65I GGTACC 1 cut(s) 490
AccB1I GGYRCC 1 cut(s) 490
AccB7I CCANNNNNTGG 3 cut(s) 1099, 3154, 5535
AccI GTMKAC 4 cut(s) 716, 2787, 2797, 5380
AccII CGCG 1 cut(s) 34
AclWI GGATC 8 cut(s) 245, 258, 1561, 2048, 2529, 4582, 4837, 5150
AcoI YGGCCR 6 cut(s) 35, 438, 1337, 2980, 4189, 4849
AcuI CTGAAG 7 cut(s) 552, 1570, 1786, 2272, 2334, 3705, 5444
AcyI GRCGYC 4 cut(s) 1581, 2748, 4447, 4552
AdeI CACNNNGTG 3 cut(s) 2416, 4918, 5535
AfeI AGCGCT 1 cut(s) 639
AfiI CCNNNNNNNGG 8 cut(s) 33, 101, 1099, 2299, 3154, 4034, 4684, 5535
AflII CTTAAG 1 cut(s) 4264
AflIII ACRYGT 1 cut(s) 3748
AjiI CACGTC 1 cut(s) 269
AjnI CCWGG 8 cut(s) 440, 1218, 2214, 3372, 3522, 3868, 4590, 5033
AjuI GAANNNNNNNTTGG 2 cut(s) 2396, 2428
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
Alw21I GWGCWC 4 cut(s) 11, 480, 649, 5352
Alw26I GTCTC 4 cut(s) 2702, 3687, 4118, 5692
AlwI GGATC 8 cut(s) 245, 258, 1561, 2048, 2529, 4582, 4837, 5150
AlwNI CAGNNNCTG 1 cut(s) 1727
Ama87I CYCGRG 1 cut(s) 3690
Aor51HI AGCGCT 1 cut(s) 639
ArsI GACNNNNNNTTYG 4 cut(s) 258, 280, 290, 312
Asp700I GAANNNNTTC 4 cut(s) 1799, 2822, 3273, 4090
Asp718I GGTACC 1 cut(s) 490
AspA2I CCTAGG 1 cut(s) 197
AspLEI GCGC 4 cut(s) 640, 1941, 3171, 5431
AspS9I GGNCC 6 cut(s) 18, 1401, 2328, 3376, 3872, 4675
AsuC2I CCSGG 2 cut(s) 167, 5761
AsuHPI GGTGA 7 cut(s) 1055, 2657, 3677, 3842, 3902, 4742, 5139
AsuII TTCGAA 1 cut(s) 2434
AsuNHI GCTAGC 3 cut(s) 2062, 2588, 2884
AvaI CYCGRG 1 cut(s) 3690
AvaII GGWCC 6 cut(s) 18, 1401, 2328, 3376, 3872, 4675
AvrII CCTAGG 1 cut(s) 197
BaeGI GKGCMC 3 cut(s) 3147, 3613, 5542
BaeI ACNNNNGTAYC 2 cut(s) 474, 507
BalI TGGCCA 4 cut(s) 440, 1339, 2982, 4191
BamHI GGATCC 1 cut(s) 250
BanI GGYRCC 1 cut(s) 490
BanII GRGCYC 2 cut(s) 11, 1969
BauI CACGAG 1 cut(s) 3933
BbsI GAAGAC 6 cut(s) 1576, 1921, 2002, 2502, 3782, 5641
Bbv12I GWGCWC 4 cut(s) 11, 480, 649, 5352
BbvCI CCTCAGC 1 cut(s) 4169
BceAI ACGGC 3 cut(s) 118, 4410, 4836
BciT130I CCWGG 8 cut(s) 442, 1220, 2216, 3374, 3524, 3870, 4592, 5035
BciVI GTATCC 3 cut(s) 1374, 3530, 3747
BclI TGATCA 2 cut(s) 2502, 2973
BcnI CCSGG 2 cut(s) 167, 5761
BcoDI GTCTC 4 cut(s) 2702, 3687, 4118, 5692
BfoI RGCGCY 2 cut(s) 641, 5432
BfrI CTTAAG 1 cut(s) 4264
BfuAI ACCTGC 2 cut(s) 502, 3345
BfuI GTATCC 3 cut(s) 1374, 3530, 3747
BlnI CCTAGG 1 cut(s) 197
BlpI GCTNAGC 1 cut(s) 1968
Bme18I GGWCC 6 cut(s) 18, 1401, 2328, 3376, 3872, 4675
BmeT110I CYCGRG 1 cut(s) 3690
BmgBI CACGTC 1 cut(s) 269
BmgT120I GGNCC 6 cut(s) 18, 1401, 2328, 3376, 3872, 4675
BmiI GGNNCC 7 cut(s) 19, 252, 492, 974, 1869, 2284, 5678
BmrI ACTGGG 1 cut(s) 4670
BmtI GCTAGC 3 cut(s) 2066, 2592, 2888
BmuI ACTGGG 1 cut(s) 4670
BpiI GAAGAC 6 cut(s) 1576, 1921, 2002, 2502, 3782, 5641
BpmI CTGGAG 1 cut(s) 4724
Bpu10I CCTNAGC 1 cut(s) 4169
Bpu1102I GCTNAGC 1 cut(s) 1968
Bpu14I TTCGAA 1 cut(s) 2434
BpuEI CTTGAG 3 cut(s) 464, 3704, 5180
BpuMI CCSGG 2 cut(s) 167, 5761
BsaAI YACGTR 2 cut(s) 1175, 3749
BsaBI GATNNNNATC 1 cut(s) 2520
BsaHI GRCGYC 4 cut(s) 1581, 2748, 4447, 4552
BsaWI WCCGGW 2 cut(s) 427, 4082
BsaXI ACNNNNNCTCC 6 cut(s) 3026, 3056, 3221, 3251, 5631, 5661
Bsc4I CCNNNNNNNGG 8 cut(s) 33, 101, 1099, 2299, 3154, 4034, 4684, 5535
Bse118I RCCGGY 1 cut(s) 5656
Bse1I ACTGG 8 cut(s) 1388, 1420, 1940, 2566, 4665, 4852, 5081, 5316
Bse3DI GCAATG 4 cut(s) 1017, 1138, 2190, 2442
Bse8I GATNNNNATC 1 cut(s) 2520
BseBI CCWGG 8 cut(s) 442, 1220, 2216, 3374, 3524, 3870, 4592, 5035
BseJI GATNNNNATC 1 cut(s) 2520
BseLI CCNNNNNNNGG 8 cut(s) 33, 101, 1099, 2299, 3154, 4034, 4684, 5535
BseMI GCAATG 4 cut(s) 1017, 1138, 2190, 2442
BseMII CTCAG 8 cut(s) 615, 855, 1672, 1982, 2471, 2728, 4160, 5703
BseNI ACTGG 8 cut(s) 1388, 1420, 1940, 2566, 4665, 4852, 5081, 5316
BseRI GAGGAG 1 cut(s) 5342
BseSI GKGCMC 3 cut(s) 3147, 3613, 5542
BseX3I CGGCCG 1 cut(s) 35
BseYI CCCAGC 2 cut(s) 4600, 5611
Bsh1236I CGCG 1 cut(s) 34
Bsh1285I CGRYCG 1 cut(s) 38
BshNI GGYRCC 1 cut(s) 490
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 4 cut(s) 11, 480, 649, 5352
BsiHKCI CYCGRG 1 cut(s) 3690
BsiSI CCGG 5 cut(s) 166, 428, 4083, 5657, 5760
BslFI GGGAC 3 cut(s) 31, 329, 3468
BslI CCNNNNNNNGG 8 cut(s) 33, 101, 1099, 2299, 3154, 4034, 4684, 5535
BsmAI GTCTC 4 cut(s) 2702, 3687, 4118, 5692
BsmFI GGGAC 3 cut(s) 31, 329, 3468
BsoBI CYCGRG 1 cut(s) 3690
Bsp119I TTCGAA 1 cut(s) 2434
Bsp1286I GDGCHC 8 cut(s) 11, 480, 649, 1969, 3147, 3613, 5352, 5542
Bsp1407I TGTACA 2 cut(s) 895, 1227
Bsp1720I GCTNAGC 1 cut(s) 1968
Bsp19I CCATGG 3 cut(s) 223, 246, 4683
BspCNI CTCAG 8 cut(s) 616, 856, 1671, 1981, 2470, 2727, 4161, 5702
BspFNI CGCG 1 cut(s) 34
BspHI TCATGA 4 cut(s) 1608, 3198, 4324, 5457
BspLI GGNNCC 7 cut(s) 19, 252, 492, 974, 1869, 2284, 5678
BspMAI CTGCAG 2 cut(s) 3342, 4755
BspMI ACCTGC 2 cut(s) 502, 3345
BspOI GCTAGC 3 cut(s) 2066, 2592, 2888
BspPI GGATC 8 cut(s) 245, 258, 1561, 2048, 2529, 4582, 4837, 5150
BspQI GCTCTTC 3 cut(s) 1670, 5611, 5717
BspT104I TTCGAA 1 cut(s) 2434
BspT107I GGYRCC 1 cut(s) 490
BspTI CTTAAG 1 cut(s) 4264
BsrDI GCAATG 4 cut(s) 1017, 1138, 2190, 2442
BsrFI RCCGGY 1 cut(s) 5656
BsrGI TGTACA 2 cut(s) 895, 1227
BsrI ACTGG 8 cut(s) 1388, 1420, 1940, 2566, 4665, 4852, 5081, 5316
BssAI RCCGGY 1 cut(s) 5656
BssNAI GTATAC 1 cut(s) 2798
BssNI GRCGYC 4 cut(s) 1581, 2748, 4447, 4552
BssSI CACGAG 1 cut(s) 3933
BssT1I CCWWGG 8 cut(s) 21, 197, 223, 246, 3300, 4617, 4683, 4725
Bst1107I GTATAC 1 cut(s) 2798
Bst2BI CACGAG 1 cut(s) 3933
Bst2UI CCWGG 8 cut(s) 442, 1220, 2216, 3374, 3524, 3870, 4592, 5035
Bst6I CTCTTC 6 cut(s) 426, 1670, 1984, 5105, 5611, 5717
BstACI GRCGYC 4 cut(s) 1581, 2748, 4447, 4552
BstAFI CTTAAG 1 cut(s) 4264
BstAPI GCANNNNNTGC 2 cut(s) 298, 4880
BstAUI TGTACA 2 cut(s) 895, 1227
BstBAI YACGTR 2 cut(s) 1175, 3749
BstBI TTCGAA 1 cut(s) 2434
BstDSI CCRYGG 5 cut(s) 223, 246, 4683, 4852, 5212
BstFNI CGCG 1 cut(s) 34
BstH2I RGCGCY 2 cut(s) 641, 5432
BstHHI GCGC 4 cut(s) 640, 1941, 3171, 5431
BstMAI GTCTC 4 cut(s) 2702, 3687, 4118, 5692
BstMCI CGRYCG 1 cut(s) 38
BstNI CCWGG 8 cut(s) 442, 1220, 2216, 3374, 3524, 3870, 4592, 5035
BstNSI RCATGY 3 cut(s) 3208, 3235, 5244
BstSLI GKGCMC 3 cut(s) 3147, 3613, 5542
BstUI CGCG 1 cut(s) 34
BstV2I GAAGAC 6 cut(s) 1576, 1921, 2002, 2502, 3782, 5641
BstX2I RGATCY 6 cut(s) 250, 1553, 2521, 4587, 4829, 5142
BstXI CCANNNNNNTGG 1 cut(s) 4690
BstYI RGATCY 6 cut(s) 250, 1553, 2521, 4587, 4829, 5142
BstZ17I GTATAC 1 cut(s) 2798
BstZI CGGCCG 1 cut(s) 35
BsuI GTATCC 3 cut(s) 1374, 3530, 3747
BtgI CCRYGG 5 cut(s) 223, 246, 4683, 4852, 5212
BtrI CACGTC 1 cut(s) 269
BtsI GCAGTG 3 cut(s) 2015, 2697, 4719
BveI ACCTGC 2 cut(s) 502, 3345
CaiI CAGNNNCTG 1 cut(s) 1727
CciI TCATGA 4 cut(s) 1608, 3198, 4324, 5457
CciNI GCGGCCGC 1 cut(s) 35
CfoI GCGC 4 cut(s) 640, 1941, 3171, 5431
Cfr10I RCCGGY 1 cut(s) 5656
Cfr13I GGNCC 6 cut(s) 18, 1401, 2328, 3376, 3872, 4675
CseI GACGC 6 cut(s) 135, 298, 2756, 2906, 3569, 4436
CsiI ACCWGGT 1 cut(s) 1218
DraI TTTAAA 2 cut(s) 691, 1125
DraIII CACNNNGTG 3 cut(s) 2416, 4918, 5535
DrdI GACNNNNNNGTC 1 cut(s) 858
DseDI GACNNNNNNGTC 1 cut(s) 858
EaeI YGGCCR 6 cut(s) 35, 438, 1337, 2980, 4189, 4849
EagI CGGCCG 1 cut(s) 35
Eam1104I CTCTTC 6 cut(s) 426, 1670, 1984, 5105, 5611, 5717
EarI CTCTTC 6 cut(s) 426, 1670, 1984, 5105, 5611, 5717
EciI GGCGGA 1 cut(s) 1840
Ecl136II GAGCTC 1 cut(s) 9
EclXI CGGCCG 1 cut(s) 35
Eco130I CCWWGG 8 cut(s) 21, 197, 223, 246, 3300, 4617, 4683, 4725
Eco147I AGGCCT 1 cut(s) 5736
Eco24I GRGCYC 2 cut(s) 11, 1969
Eco32I GATATC 1 cut(s) 2944
Eco47I GGWCC 6 cut(s) 18, 1401, 2328, 3376, 3872, 4675
Eco47III AGCGCT 1 cut(s) 639
Eco52I CGGCCG 1 cut(s) 35
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 7 cut(s) 552, 1570, 1786, 2272, 2334, 3705, 5444
Eco88I CYCGRG 1 cut(s) 3690
EcoICRI GAGCTC 1 cut(s) 9
EcoO109I RGGNCCY 1 cut(s) 3872
EcoRI GAATTC 2 cut(s) 3676, 5418
EcoRII CCWGG 8 cut(s) 440, 1218, 2214, 3372, 3522, 3868, 4590, 5033
EcoRV GATATC 1 cut(s) 2944
EcoT14I CCWWGG 8 cut(s) 21, 197, 223, 246, 3300, 4617, 4683, 4725
EcoT22I ATGCAT 4 cut(s) 687, 1672, 2001, 4975
EcoT38I GRGCYC 2 cut(s) 11, 1969
ErhI CCWWGG 8 cut(s) 21, 197, 223, 246, 3300, 4617, 4683, 4725
FalI AAGNNNNNCTT 4 cut(s) 2396, 2428, 3261, 3293
FaqI GGGAC 3 cut(s) 31, 329, 3468
FauI CCCGC 1 cut(s) 8
FauNDI CATATG 2 cut(s) 1037, 1615
FbaI TGATCA 2 cut(s) 2502, 2973
FblI GTMKAC 4 cut(s) 716, 2787, 2797, 5380
FriOI GRGCYC 2 cut(s) 11, 1969
GlaI GCGC 4 cut(s) 639, 1940, 3170, 5430
GsaI CCCAGC 2 cut(s) 4604, 5615
GsuI CTGGAG 1 cut(s) 4724
HaeII RGCGCY 2 cut(s) 641, 5432
HapII CCGG 5 cut(s) 166, 428, 4083, 5657, 5760
HgaI GACGC 6 cut(s) 135, 298, 2756, 2906, 3569, 4436
HhaI GCGC 4 cut(s) 640, 1941, 3171, 5431
Hin1I GRCGYC 4 cut(s) 1581, 2748, 4447, 4552
Hin6I GCGC 4 cut(s) 638, 1939, 3169, 5429
HinP1I GCGC 4 cut(s) 638, 1939, 3169, 5429
HincII GTYRAC 2 cut(s) 717, 3732
HindII GTYRAC 2 cut(s) 717, 3732
HindIII AAGCTT 3 cut(s) 1047, 3863, 3987
HpaI GTTAAC 1 cut(s) 3732
HpaII CCGG 5 cut(s) 166, 428, 4083, 5657, 5760
HphI GGTGA 7 cut(s) 1055, 2657, 3677, 3842, 3902, 4742, 5139
Hpy99I CGWCG 6 cut(s) 100, 110, 139, 3563, 4035, 4452
HpyCH4IV ACGT 7 cut(s) 268, 1174, 1581, 3734, 3748, 4552, 5006
HpySE526I ACGT 7 cut(s) 268, 1174, 1581, 3734, 3748, 4552, 5006
Hsp92I GRCGYC 4 cut(s) 1581, 2748, 4447, 4552
HspAI GCGC 4 cut(s) 638, 1939, 3169, 5429
KpnI GGTACC 1 cut(s) 494
Ksp22I TGATCA 2 cut(s) 2502, 2973
KspAI GTTAAC 1 cut(s) 3732
LguI GCTCTTC 3 cut(s) 1670, 5611, 5717
MabI ACCWGGT 1 cut(s) 1218
MaeII ACGT 7 cut(s) 268, 1174, 1581, 3734, 3748, 4552, 5006
MaeIII GTNAC 9 cut(s) 1687, 1945, 3227, 3704, 3830, 4135, 4516, 4910, 5341
MfeI CAATTG 2 cut(s) 1001, 3885
MflI RGATCY 6 cut(s) 250, 1553, 2521, 4587, 4829, 5142
MhlI GDGCHC 8 cut(s) 11, 480, 649, 1969, 3147, 3613, 5352, 5542
MlsI TGGCCA 4 cut(s) 440, 1339, 2982, 4191
MluNI TGGCCA 4 cut(s) 440, 1339, 2982, 4191
MlyI GAGTC 7 cut(s) 300, 1552, 1822, 2197, 2348, 3247, 4968
Mox20I TGGCCA 4 cut(s) 440, 1339, 2982, 4191
Mph1103I ATGCAT 4 cut(s) 687, 1672, 2001, 4975
MroXI GAANNNNTTC 4 cut(s) 1799, 2822, 3273, 4090
MscI TGGCCA 4 cut(s) 440, 1339, 2982, 4191
MslI CAYNNNNRTG 9 cut(s) 1374, 1607, 2004, 2239, 3140, 3203, 3959, 4688, 4742
Msp20I TGGCCA 4 cut(s) 440, 1339, 2982, 4191
MspA1I CMGCKG 5 cut(s) 15, 4042, 4168, 4750, 4756
MspCI CTTAAG 1 cut(s) 4264
MspI CCGG 5 cut(s) 166, 428, 4083, 5657, 5760
MunI CAATTG 2 cut(s) 1001, 3885
MvaI CCWGG 8 cut(s) 442, 1220, 2216, 3374, 3524, 3870, 4592, 5035
MvnI CGCG 1 cut(s) 34
NciI CCSGG 2 cut(s) 167, 5761
NcoI CCATGG 3 cut(s) 223, 246, 4683
NdeI CATATG 2 cut(s) 1037, 1615
NheI GCTAGC 3 cut(s) 2062, 2588, 2884
NlaIV GGNNCC 7 cut(s) 19, 252, 492, 974, 1869, 2284, 5678
NmeAIII GCCGAG 1 cut(s) 2666
NmuCI GTSAC 6 cut(s) 1687, 1945, 3227, 3830, 4135, 4910
NotI GCGGCCGC 1 cut(s) 35
NsiI ATGCAT 4 cut(s) 687, 1672, 2001, 4975
NspI RCATGY 3 cut(s) 3208, 3235, 5244
NspV TTCGAA 1 cut(s) 2434
PagI TCATGA 4 cut(s) 1608, 3198, 4324, 5457
PceI AGGCCT 1 cut(s) 5736
PciSI GCTCTTC 3 cut(s) 1670, 5611, 5717
PcsI WCGNNNNNNNCGW 3 cut(s) 143, 274, 315
PdmI GAANNNNTTC 4 cut(s) 1799, 2822, 3273, 4090
PflMI CCANNNNNTGG 3 cut(s) 1099, 3154, 5535
PfoI TCCNGGA 3 cut(s) 3372, 3522, 4590
PleI GAGTC 7 cut(s) 300, 1551, 1821, 2197, 2347, 3247, 4967
PpsI GAGTC 7 cut(s) 300, 1551, 1821, 2197, 2347, 3247, 4967
Ppu21I YACGTR 2 cut(s) 1175, 3749
PpuMI RGGWCCY 1 cut(s) 3872
PsiI TTATAA 5 cut(s) 1205, 1842, 2477, 2957, 4316
Psp124BI GAGCTC 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 3872
Psp6I CCWGG 8 cut(s) 440, 1218, 2214, 3372, 3522, 3868, 4590, 5033
PspFI CCCAGC 2 cut(s) 4600, 5611
PspGI CCWGG 8 cut(s) 440, 1218, 2214, 3372, 3522, 3868, 4590, 5033
PspN4I GGNNCC 7 cut(s) 19, 252, 492, 974, 1869, 2284, 5678
PspPI GGNCC 6 cut(s) 18, 1401, 2328, 3376, 3872, 4675
PspPPI RGGWCCY 1 cut(s) 3872
PstI CTGCAG 2 cut(s) 3342, 4755
PstNI CAGNNNCTG 1 cut(s) 1727
PsuI RGATCY 6 cut(s) 250, 1553, 2521, 4587, 4829, 5142
PvuII CAGCTG 2 cut(s) 4750, 4756
RseI CAYNNNNRTG 9 cut(s) 1374, 1607, 2004, 2239, 3140, 3203, 3959, 4688, 4742
SacI GAGCTC 1 cut(s) 11
SalI GTCGAC 1 cut(s) 715
SapI GCTCTTC 3 cut(s) 1670, 5611, 5717
Sau96I GGNCC 6 cut(s) 18, 1401, 2328, 3376, 3872, 4675
SchI GAGTC 7 cut(s) 300, 1552, 1822, 2197, 2348, 3247, 4968
SduI GDGCHC 8 cut(s) 11, 480, 649, 1969, 3147, 3613, 5352, 5542
SexAI ACCWGGT 1 cut(s) 1218
SfuI TTCGAA 1 cut(s) 2434
SinI GGWCC 6 cut(s) 18, 1401, 2328, 3376, 3872, 4675
SmiMI CAYNNNNRTG 9 cut(s) 1374, 1607, 2004, 2239, 3140, 3203, 3959, 4688, 4742
SmlI CTYRAG 4 cut(s) 479, 3719, 4264, 5195
SmoI CTYRAG 4 cut(s) 479, 3719, 4264, 5195
SseBI AGGCCT 1 cut(s) 5736
SspI AATATT 6 cut(s) 1569, 1698, 2179, 2253, 3715, 4056
SstI GAGCTC 1 cut(s) 11
StuI AGGCCT 1 cut(s) 5736
StyI CCWWGG 8 cut(s) 21, 197, 223, 246, 3300, 4617, 4683, 4725
TaiI ACGT 7 cut(s) 271, 1177, 1584, 3737, 3751, 4555, 5009
TatI WGTACW 7 cut(s) 463, 895, 1227, 2682, 3402, 3489, 4899
TauI GCSGC 5 cut(s) 37, 40, 219, 2095, 4236
TseFI GTSAC 6 cut(s) 1687, 1945, 3227, 3830, 4135, 4910
Tsp45I GTSAC 6 cut(s) 1687, 1945, 3227, 3830, 4135, 4910
TspGWI ACGGA 9 cut(s) 113, 632, 2526, 2816, 4563, 4821, 5052, 5117, 5201
Van91I CCANNNNNTGG 3 cut(s) 1099, 3154, 5535
Vha464I CTTAAG 1 cut(s) 4264
VpaK11BI GGWCC 6 cut(s) 18, 1401, 2328, 3376, 3872, 4675
XbaI TCTAGA 2 cut(s) 3467, 3505
XceI RCATGY 3 cut(s) 3208, 3235, 5244
XcmI CCANNNNNNNNNTGG 1 cut(s) 5020
XmaJI CCTAGG 1 cut(s) 197
XmiI GTMKAC 4 cut(s) 716, 2787, 2797, 5380
XmnI GAANNNNTTC 4 cut(s) 1799, 2822, 3273, 4090
ZraI GACGTC 2 cut(s) 1582, 4553
Zsp2I ATGCAT 4 cut(s) 687, 1672, 2001, 4975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.