RchiOBHm_Chr2g0151731

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0009173
Physical Location & Seq
Reverse (-)
69291421 .. 69307387
15967 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52090

Sequence Viewer

Length: 5823 bp
ATGGCGAGCTCCAGCGGGACCAAGGCCGATGCGCGGCCGCAGAGGTCGCTGTCGAGGGGAATGACGAGAATGGCTACTAGAGTTGTGGACTTTCCGACGGAGGGCGACGATGCGTTGGACAGTGGAGTTGTGCCGTCGTGCCTTGCGGCTATTGCTCCGATTTTCCGGGTTGCCAATGAGATTGAGAAGGACAACCCTAGGGTTGCTTATCTCTGCCGCTTCCATGGATTTGAAAAGGCGCATACCATGGATCCAACATCAAGTGGACGTGGAGTTCGTCAGTTTAAGACGCATCTTTTGCACAGACTCGAAAGGGACGAAGTAGATACAAAACATCAGCTTGCTAAATCTGATACAAAAGAAATCATGTTGTATTACCACCAATTCTACGAGAAAAATATCAGAGATGGAGAATATACCAAGAAACCGGAAGAGATGGCCAGGATTTGTCAGATTGCAACTGTACTGTATGATGTGCTCAGGACAGTGGTACCTGCTTCTCAAATAGACATACAGACCCAGAGAATTGCTGAAGATGTCAAGAGCAAAAGGGAGCAATATGTAAACTATAACATTCTTCCATTATATACTGTCGGGGTTAAACCGACAATTATGGAACTTCCTGAGATCAAAGCAGCGCTCCGTGCTCTACAGAATGTGAGTAATCTTCCAATGCCAAGAATGCAATTTAAACCCACGAATCCTGATGACAAGTCGACAATGCCTACAGAGAGGATTAAACCTGTGAATGATATACTCGATTGGCTTTCGGCTATTTTTGGGTTTCAGAAAGGAAATGTGGCAAATCAGAGGGAACATTTAATTTTGTTGCTTGCCAACATTGATGTAAGACATAGAAGTCCTGAGAACTACAATGAGTTGAATAGTGCAACTGTACAGCATTTGATGGAGAAATTCTTTAAGAATTATCGTTCATGGTATAACTATCTGCATTGTAAAACAAATCTTCGGTTCCCCGAAGGTTCTCACAGCAAACAACAATTGGAACTTATATACATTGCTCTCTATCTTCTCATATGGGGTGAAGCTTCAAATGTCCGATTCATGCCTGAATGCTTATGCTACATTTTCCATAATATGGCGAACGAGGTCTATGGAATTTTAAATAGCAATGCTCATACTGTTAGTGGAGAAACATACCAAACAGTTGTACGTGATGAAGAATCTTTTTTGAGGGATGTTATAACTCCTATCTACCAGGTTTTGTACAAGGAAGCAAAGAGAAACAAAATGGGCAAGGCAAGTCATTCAAGATGGAGAAATTATGATGATTTGAATGAATACTTCTGGTCGGACAAATGTTTTAAGCTAGGGTGGCCAATGGATGCTAAAGCAGATTTCTTTAGGCATTCAGATGGGATACAACCAGTAAATGAGAGGACCAATCAAGCTACAGGTGGTAGGAGGAAGCCTAAAACAAATTTTGTAGAAGTCCGAACATTTTTACACCTCTACAGAAGTTTTGATCGAATGTGGATATTCTTCATATTGGCTTTACAGGCTATGATTATTATTGCATGGAGTCCTTCTGGATCTATCACTTCAATATTTAATGCAGACGTCTTCAGAAGTGTTTTAAGCATCTTCATGTCATATGCTTTCCTTAATTTATTACAAGCCACTTTGGACATAATTCTCAGTTGGAATGCATGGAAGAGCTTGAAAGTCACTCAAATATTACGATACCTCTTGAAATTTGCAGTAGCTGTTGTCTGGGCTGTGGTTCTGCCTATTGGTTATTCTAGTTCTATGCAAAATCCTACAGGGCTTCTGAAATTCTTCAGTAGTTGGAGTCGGGATTGGCGGAATCAGTCATTTTATAATTATGCTGTTGCAATCTATATGGTTCCTAATTTATTGGCTGCTATGCTATTTTTTCTTCCACCTTTACGAAGACACATAGAGCGTTCCAACTGGCGCATTGTCACTCTTTTTATGTGGTGGGCTCAGGCAAGAATTTCTTTCTTTGGTCTAACCAAACCAAAACTGTATGTCGGAAGAGGAATGCATGAGGATGTCTTCTCACTGCTGAAATATACATTGTTCTGGATCATGCTGCTAATCAGCAAGCTAGCGTTCAGCTATTATGTGGAGATATTGCCGCTTGTTGCACCAACGAAGTTGATAATGGATATGCCTATCAGTAATTACCAATGGCATGAGTTCTTTCCGAATGCAACGCATAATATTGGTGTTGTCATTGCAATATGGACTCCAATTATCCTGGTATATTTTATGGATGCACAAATATGGTATGCAATATTTTCTACTCTGTTTGGTGGGATTCACGGAGCCTTCAGCCATTTGGGGGAGATACGAACACTTGGGATGTTGCGGTCCAGATTTGAGTCTGTGCCTTCAGCTTTCAGTGATCGGCTTATGCCATCGTCAGAGAATAAGGATGCCAGAAAGAAGAAACACTTGGTGGATGAAGGACTTGTTCGAAAGAACATTGCTAATTTCTCTCAGGTCTGGAATGAATTTATAAATTCTATGCGAATGGAAGACCTGATCAGCAATAGGGATAGGGATCTGCTGCTTGTTCCGTATTCTTCAAGTGATGTTTCTGTCGTCCAGTGGCCTCCTTTCTTGCTGGCTAGCAAGATTCCAATAGCATTAGACATGGCAAAGGATTTTACAGGAAAGTCGGATGATGATTTATTCAGGAAGATTAGGAATGATGATTATATGTACTCGGCAGTGATTGAATGCTATGAAACACTCAGGGATATTATATTTGGCCTTCTTGATGATGACGCCGATAAGATGATTGTAAGGCAGATATGTTATGAAGTAGACAGTAGTATACAGCAGCAGAGGTTTTTGATTAATTTCCGTATGAGTGGGCTGCCATTTCTTAGTGAGAGGTTGGAGAAGTTTCTCAAACTCTTGCTAGCCGAAGATGAGAATGTCGAAAATTCCTTGCGTCAGATAATCAATGTCCTTCAAGATATCATGGAGATTATAACACAGGATGTAATGATCAATGGCCATGAAATCCTCGAAGCAGCTCACTACATAGAGGGTCAAAATGTCAAGAAGGAGCAAAGGTTTCAAAAAATCAACATATATCTTGCCCAAAATCCATCTTGGAGAGAGAAGGTCGTTAGGCTTCATTTGCTTTTGACCGTCAAGGAATCTGCCATCAATGTGCCCCAAAACTTGGATGCTCGCAGGCGCATTACTTTCTTTGCGAACTCCTTATTCATGAACATGCCAAATGCTCCCAAAGTTCGTGACATGCTCTCCTTTAGTGTTCTGACTCCTTATTACAAAGAAGATGTTCTTTATTCTGATGAGGAACTTACCAAGGAAAATGAGGATGGAATCTCAATTTTGTTCTACCTGCAGAAGATATATCCTGATGAATGGACAAATTTCCTGGACCGCATAAATGAACAAAAAAGTCATTACACTGAAAAGGACAAGTCGGAGTTAATTCGTCAGTGGGTATCTTACAGGGGACAGACACTTTCTAGAACAGTGAGAGGGATGATGTACTATAGGAAGGCTCTAGACCTTCAATGTATCCTGGAAACAGCAGGAGATGGTGGTGTTTATGGAGGCGACGCAGCCCATATTTTAAGTGATAAAGAAGAGAAGGATATTCTTGATCGGGCACAAGCTCTGGCAGATTTGAAGTTCACTTATGTAGTTTCTTGTCAGGTCTATGGTGAACAGAAGAATTCCACTGAAGCCCGAGACAAAAGTTGTTACACTAATATTCTCAAGCTGATGTTAACGTATCCATCTCTACGTGTTGCTTACATAGATACAAGAGAGAAGACAGTGAATGGAAAATCCCAAAAGGTTCATTTCTCTGTTCTGGTCAAGGGAGGTGACAAATGGGATGAGGAAATATACCGCATCAAGCTTCCAGGTCCTCCAACAGTAATTGGTGAAGGAAAACCCGAAAATCAAAACCATGCCATCGTATTTACTCGTGGAGAAGCCCTGCAAACCATTGACATGAATCAGGATAATTATTTTGAGGAAGCTTTCAAAATGAGAAATGTATTGGAGGAATTTCTAAAGCCTCGACGGGGGCAGCGGAAACCTACAATATTGGGTCTAAGGGAACATATATTTACCGGAAGTGTTTCATCACTCGCTTGGTTCATGTCCAATCAAGAGACAAGTTTTGTGACTATAGGCCAACGAATTTTGGCAAATCCGCTGAGGGTACGGTTTCATTATGGCCATCCTGATATTTTTGACAGAATCTTTCACATAACAAGAGGCGGCATAAGCAAAGCATCAAAAGTAATTAACTTAAGCGAGGATATATTTTCAGGATATAATTCTGTTCTGCGTGGGGGATTTATAACACATCATGAGTATATCCAAGTAGGCAAGGGGCGTGATGTTGGAATGAATCAAATATCGCTTTTCGAGGCTAAAGTTGCAAATGGAAATGGAGAGCAGACACTTAGCCGTGATGTTTATAGGCTTGGGCGTCGGTTTGATTTCTATAGAATGCTGTCTTTTTACTTCACAACAGTTGGGTTCTATTTTAGTAGTATGGTAACTGTGCTGACTGTCTATGTGTTTTTATATGGACGTCTATATATGGTTATGAGTGGATTGGAAAGTGAGATCCTGGATAGCCCAGCCATACATCAGACCAAGGCTTTTGAACAAGCTCTGGCTACACAGTCTGTCTTTCAACTGGGCTTGTTGCTGGTCCTTCCCATGGTTATGGAAATTGGTCTGGAGAAAGGATTTCGCACTGCCTTGGGTGATTTCATCATCATGCAGCTGCAGCTGGCCTCTGTTTTCTTCACTTTCCAACTAGGAACAAAAGCACATTACTACGGAAGGACAATCTTACATGGAGGATCTAAATATCGAGCAACTGGCCGTGGTTTTGTTGTTTTTCATGCAAAGTTTGCCGATAACTACAGGCTGTACTCACGAAGTCACTTTGTGAAAGGGTTGGAGCTGTTGATTCTGCTGATTGTGTATGGAGTCTATGGGACTGCATATAGGAGTTCAAATCTGTATTTTTTCATCACGTTCTCCATGTGGTTTCTGGTTGCTTCCTGGTTGTTTGCTCCTTTTATCTTTAATCCGTCCAGCTTTGACTGGCAAAAAACTGTAGATGATTGGACTGATTGGAAGAGGTGGATGGGAAATCGTGGTGGTATTGGGATCTCACCTGACAAAAGTTGGGAATCATGGTGGGATGAAGAACAGGAACACCTCAAGCACACTATTATCCGTGGAAGAATTATTGAGATAATTCTTGCATGTCGCTTCTTTTTTTACCAATATGGGATTGTTTACCACCTCGATATAGCTCATTTCAGCAAGAGTTTACTGGTTTATGGACTTTCTTGGGTGGTTATGATAACGGTGCTCCTTGTCTTAAAGATGGTGTCAATGGGTAGACGAAGATTTGGCACTGACTTTCAGCTCATGTTCAGAATTCTGAAGGCGCTTCTTTTTCTTGGCTTCATGTCAGTCATGACTGTCCTATTTGTAGTTTGTGGCCTTACTATTTCTGATCTATTTGCTGCTATTCTTGCCTTCTTGCCCACAGGGTGGGCACTACTTCTGATCGGGCAAGTATGCAGGCCAATGGTTAAGGGAGTAGGATTCTGGGAATCAATTAAGGAGCTGGGAAGAGCCTATGATTACATAATGGGAGTGGTAGTCTTCATGCCGGTTGCCATTTTGTCATGGTTCCCATTTGTCTCAGAGTTCCAAACACGGTTGCTCTTCAACCAAGCATTCAGTCGAGGCCTACAAATTTCGATGATTCTCGCCGGGAGGAAAGATAAGACAGCTTCTAATAGATAG

Protein Analysis

1940

Amino Acids

224.77

Weight (kDa)

9.03

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 51 - 180 4.1e-10 Vta1 like
FKS1_dom1 PF14288 336 - 447 1.8e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 868 - 1051 1.3e-69 Callose synthase 1 domain
Glucan_synthase PF02364 1058 - 1148 1.5e-29 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1151 - 1752 2.8e-201 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 1205, 1842, 2507, 2987, 4346
AasI GACNNNNNNGTC 1 cut(s) 858
AatII GACGTC 2 cut(s) 1584, 4585
Acc36I ACCTGC 2 cut(s) 502, 3375
Acc65I GGTACC 1 cut(s) 490
AccB1I GGYRCC 1 cut(s) 490
AccB7I CCANNNNNTGG 3 cut(s) 1099, 3184, 5565
AccI GTMKAC 4 cut(s) 716, 2817, 2827, 5410
AccII CGCG 1 cut(s) 34
AclWI GGATC 8 cut(s) 245, 258, 1561, 2078, 2559, 4612, 4867, 5180
AcoI YGGCCR 6 cut(s) 35, 438, 1337, 3010, 4219, 4879
AcuI CTGAAG 7 cut(s) 552, 1570, 1786, 2302, 2364, 3735, 5474
AcyI GRCGYC 4 cut(s) 1581, 2778, 4477, 4582
AdeI CACNNNGTG 3 cut(s) 2446, 4948, 5565
AfaI GTAC 9 cut(s) 465, 492, 897, 1173, 1229, 2714, 3521, 4206, 4931
AfeI AGCGCT 1 cut(s) 639
AfiI CCNNNNNNNGG 8 cut(s) 33, 101, 1099, 2329, 3184, 4064, 4714, 5565
AflII CTTAAG 1 cut(s) 4294
AflIII ACRYGT 1 cut(s) 3778
AjiI CACGTC 1 cut(s) 269
AjnI CCWGG 8 cut(s) 440, 1218, 2244, 3402, 3552, 3898, 4620, 5063
AjuI GAANNNNNNNTTGG 2 cut(s) 2426, 2458
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
Alw21I GWGCWC 4 cut(s) 11, 480, 649, 5382
Alw26I GTCTC 3 cut(s) 3717, 4148, 5722
AlwI GGATC 8 cut(s) 245, 258, 1561, 2078, 2559, 4612, 4867, 5180
AlwNI CAGNNNCTG 1 cut(s) 1727
Ama87I CYCGRG 1 cut(s) 3720
Aor51HI AGCGCT 1 cut(s) 639
ArsI GACNNNNNNTTYG 4 cut(s) 258, 280, 290, 312
AseI ATTAAT 1 cut(s) 2850
Asp700I GAANNNNTTC 3 cut(s) 1799, 3303, 4120
Asp718I GGTACC 1 cut(s) 490
AspA2I CCTAGG 1 cut(s) 197
AspLEI GCGC 6 cut(s) 34, 241, 640, 1941, 3201, 5461
AspS9I GGNCC 6 cut(s) 18, 1401, 2358, 3406, 3902, 4705
AsuC2I CCSGG 2 cut(s) 167, 5791
AsuHPI GGTGA 6 cut(s) 1055, 3707, 3872, 3932, 4772, 5169
AsuII TTCGAA 1 cut(s) 2464
AsuNHI GCTAGC 3 cut(s) 2092, 2618, 2914
AvaI CYCGRG 1 cut(s) 3720
AvaII GGWCC 6 cut(s) 18, 1401, 2358, 3406, 3902, 4705
AvrII CCTAGG 1 cut(s) 197
BaeGI GKGCMC 3 cut(s) 3177, 3643, 5572
BaeI ACNNNNGTAYC 2 cut(s) 474, 507
BalI TGGCCA 4 cut(s) 440, 1339, 3012, 4221
BamHI GGATCC 1 cut(s) 250
BanI GGYRCC 1 cut(s) 490
BanII GRGCYC 2 cut(s) 11, 1969
BauI CACGAG 1 cut(s) 3963
BbsI GAAGAC 6 cut(s) 1576, 1921, 2032, 2532, 3812, 5671
Bbv12I GWGCWC 4 cut(s) 11, 480, 649, 5382
BbvCI CCTCAGC 1 cut(s) 4199
BceAI ACGGC 3 cut(s) 118, 4440, 4866
BciT130I CCWGG 8 cut(s) 442, 1220, 2246, 3404, 3554, 3900, 4622, 5065
BciVI GTATCC 3 cut(s) 1374, 3560, 3777
BclI TGATCA 2 cut(s) 2532, 3003
BcnI CCSGG 2 cut(s) 167, 5791
BcoDI GTCTC 3 cut(s) 3717, 4148, 5722
BfoI RGCGCY 2 cut(s) 641, 5462
BfrI CTTAAG 1 cut(s) 4294
BfuAI ACCTGC 2 cut(s) 502, 3375
BfuI GTATCC 3 cut(s) 1374, 3560, 3777
BlnI CCTAGG 1 cut(s) 197
Bme18I GGWCC 6 cut(s) 18, 1401, 2358, 3406, 3902, 4705
BmeT110I CYCGRG 1 cut(s) 3720
BmgBI CACGTC 1 cut(s) 269
BmgT120I GGNCC 6 cut(s) 18, 1401, 2358, 3406, 3902, 4705
BmiI GGNNCC 7 cut(s) 19, 252, 492, 974, 1869, 2314, 5708
BmrI ACTGGG 1 cut(s) 4700
BmtI GCTAGC 3 cut(s) 2096, 2622, 2918
BmuI ACTGGG 1 cut(s) 4700
BpiI GAAGAC 6 cut(s) 1576, 1921, 2032, 2532, 3812, 5671
BpmI CTGGAG 1 cut(s) 4754
Bpu10I CCTNAGC 3 cut(s) 479, 1968, 4199
Bpu14I TTCGAA 1 cut(s) 2464
BpuEI CTTGAG 2 cut(s) 3734, 5210
BpuMI CCSGG 2 cut(s) 167, 5791
BsaAI YACGTR 2 cut(s) 1175, 3779
BsaBI GATNNNNATC 1 cut(s) 2550
BsaHI GRCGYC 4 cut(s) 1581, 2778, 4477, 4582
BsaWI WCCGGW 2 cut(s) 427, 4112
BsaXI ACNNNNNCTCC 6 cut(s) 3056, 3086, 3251, 3281, 5661, 5691
Bsc4I CCNNNNNNNGG 8 cut(s) 33, 101, 1099, 2329, 3184, 4064, 4714, 5565
Bse118I RCCGGY 1 cut(s) 5686
Bse1I ACTGG 7 cut(s) 1388, 1940, 2596, 4695, 4882, 5111, 5346
Bse3DI GCAATG 4 cut(s) 1017, 1138, 2220, 2472
Bse8I GATNNNNATC 1 cut(s) 2550
BseBI CCWGG 8 cut(s) 442, 1220, 2246, 3404, 3554, 3900, 4622, 5065
BseJI GATNNNNATC 1 cut(s) 2550
BseLI CCNNNNNNNGG 8 cut(s) 33, 101, 1099, 2329, 3184, 4064, 4714, 5565
BseMI GCAATG 4 cut(s) 1017, 1138, 2220, 2472
BseMII CTCAG 9 cut(s) 493, 615, 855, 1672, 1982, 2501, 2758, 4190, 5733
BseNI ACTGG 7 cut(s) 1388, 1940, 2596, 4695, 4882, 5111, 5346
BseSI GKGCMC 3 cut(s) 3177, 3643, 5572
BseX3I CGGCCG 1 cut(s) 35
BseYI CCCAGC 2 cut(s) 4630, 5641
Bsh1236I CGCG 1 cut(s) 34
Bsh1285I CGRYCG 1 cut(s) 38
BshNI GGYRCC 1 cut(s) 490
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 4 cut(s) 11, 480, 649, 5382
BsiHKCI CYCGRG 1 cut(s) 3720
BsiSI CCGG 5 cut(s) 166, 428, 4113, 5687, 5790
BslFI GGGAC 4 cut(s) 31, 329, 3498, 5011
BslI CCNNNNNNNGG 8 cut(s) 33, 101, 1099, 2329, 3184, 4064, 4714, 5565
BsmAI GTCTC 3 cut(s) 3717, 4148, 5722
BsmFI GGGAC 4 cut(s) 31, 329, 3498, 5011
BsmI GAATGC 9 cut(s) 687, 1079, 1369, 1672, 2031, 2200, 2735, 4503, 5753
BsoBI CYCGRG 1 cut(s) 3720
Bsp119I TTCGAA 1 cut(s) 2464
Bsp1286I GDGCHC 8 cut(s) 11, 480, 649, 1969, 3177, 3643, 5382, 5572
Bsp1407I TGTACA 2 cut(s) 895, 1227
Bsp19I CCATGG 3 cut(s) 223, 246, 4713
BspCNI CTCAG 9 cut(s) 492, 616, 856, 1671, 1981, 2500, 2757, 4191, 5732
BspFNI CGCG 1 cut(s) 34
BspHI TCATGA 3 cut(s) 3228, 4354, 5487
BspLI GGNNCC 7 cut(s) 19, 252, 492, 974, 1869, 2314, 5708
BspMAI CTGCAG 2 cut(s) 3372, 4785
BspMI ACCTGC 2 cut(s) 502, 3375
BspOI GCTAGC 3 cut(s) 2096, 2622, 2918
BspPI GGATC 8 cut(s) 245, 258, 1561, 2078, 2559, 4612, 4867, 5180
BspQI GCTCTTC 3 cut(s) 1670, 5641, 5747
BspT104I TTCGAA 1 cut(s) 2464
BspT107I GGYRCC 1 cut(s) 490
BspTI CTTAAG 1 cut(s) 4294
BsrDI GCAATG 4 cut(s) 1017, 1138, 2220, 2472
BsrFI RCCGGY 1 cut(s) 5686
BsrGI TGTACA 2 cut(s) 895, 1227
BsrI ACTGG 7 cut(s) 1388, 1940, 2596, 4695, 4882, 5111, 5346
BssAI RCCGGY 1 cut(s) 5686
BssNAI GTATAC 1 cut(s) 2828
BssNI GRCGYC 4 cut(s) 1581, 2778, 4477, 4582
BssSI CACGAG 1 cut(s) 3963
BssT1I CCWWGG 8 cut(s) 21, 197, 223, 246, 3330, 4647, 4713, 4755
Bst1107I GTATAC 1 cut(s) 2828
Bst2BI CACGAG 1 cut(s) 3963
Bst2UI CCWGG 8 cut(s) 442, 1220, 2246, 3404, 3554, 3900, 4622, 5065
Bst6I CTCTTC 7 cut(s) 426, 1670, 2014, 3612, 5135, 5641, 5747
BstACI GRCGYC 4 cut(s) 1581, 2778, 4477, 4582
BstAFI CTTAAG 1 cut(s) 4294
BstAPI GCANNNNNTGC 2 cut(s) 298, 4910
BstAUI TGTACA 2 cut(s) 895, 1227
BstBAI YACGTR 2 cut(s) 1175, 3779
BstBI TTCGAA 1 cut(s) 2464
BstDSI CCRYGG 5 cut(s) 223, 246, 4713, 4882, 5242
BstFNI CGCG 1 cut(s) 34
BstH2I RGCGCY 2 cut(s) 641, 5462
BstHHI GCGC 6 cut(s) 34, 241, 640, 1941, 3201, 5461
BstMAI GTCTC 3 cut(s) 3717, 4148, 5722
BstMCI CGRYCG 1 cut(s) 38
BstNI CCWGG 8 cut(s) 442, 1220, 2246, 3404, 3554, 3900, 4622, 5065
BstNSI RCATGY 3 cut(s) 3238, 3265, 5274
BstSLI GKGCMC 3 cut(s) 3177, 3643, 5572
BstUI CGCG 1 cut(s) 34
BstV2I GAAGAC 6 cut(s) 1576, 1921, 2032, 2532, 3812, 5671
BstX2I RGATCY 6 cut(s) 250, 1553, 2551, 4617, 4859, 5172
BstXI CCANNNNNNTGG 1 cut(s) 4720
BstYI RGATCY 6 cut(s) 250, 1553, 2551, 4617, 4859, 5172
BstZ17I GTATAC 1 cut(s) 2828
BstZI CGGCCG 1 cut(s) 35
BsuI GTATCC 3 cut(s) 1374, 3560, 3777
BtgI CCRYGG 5 cut(s) 223, 246, 4713, 4882, 5242
BtrI CACGTC 1 cut(s) 269
BtsI GCAGTG 3 cut(s) 2045, 2727, 4749
BveI ACCTGC 2 cut(s) 502, 3375
CaiI CAGNNNCTG 1 cut(s) 1727
CciI TCATGA 3 cut(s) 3228, 4354, 5487
CciNI GCGGCCGC 1 cut(s) 35
CfoI GCGC 6 cut(s) 34, 241, 640, 1941, 3201, 5461
Cfr10I RCCGGY 1 cut(s) 5686
Cfr13I GGNCC 6 cut(s) 18, 1401, 2358, 3406, 3902, 4705
CseI GACGC 5 cut(s) 298, 2786, 2936, 3599, 4466
CsiI ACCWGGT 1 cut(s) 1218
Csp6I GTAC 9 cut(s) 464, 491, 896, 1172, 1228, 2713, 3520, 4205, 4930
CviQI GTAC 9 cut(s) 464, 491, 896, 1172, 1228, 2713, 3520, 4205, 4930
DraI TTTAAA 2 cut(s) 691, 1125
DraIII CACNNNGTG 3 cut(s) 2446, 4948, 5565
DrdI GACNNNNNNGTC 1 cut(s) 858
DseDI GACNNNNNNGTC 1 cut(s) 858
EaeI YGGCCR 6 cut(s) 35, 438, 1337, 3010, 4219, 4879
EagI CGGCCG 1 cut(s) 35
Eam1104I CTCTTC 7 cut(s) 426, 1670, 2014, 3612, 5135, 5641, 5747
EarI CTCTTC 7 cut(s) 426, 1670, 2014, 3612, 5135, 5641, 5747
EciI GGCGGA 1 cut(s) 1840
Ecl136II GAGCTC 1 cut(s) 9
EclXI CGGCCG 1 cut(s) 35
Eco130I CCWWGG 8 cut(s) 21, 197, 223, 246, 3330, 4647, 4713, 4755
Eco147I AGGCCT 1 cut(s) 5766
Eco24I GRGCYC 2 cut(s) 11, 1969
Eco32I GATATC 1 cut(s) 2974
Eco47I GGWCC 6 cut(s) 18, 1401, 2358, 3406, 3902, 4705
Eco47III AGCGCT 1 cut(s) 639
Eco52I CGGCCG 1 cut(s) 35
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 7 cut(s) 552, 1570, 1786, 2302, 2364, 3735, 5474
Eco88I CYCGRG 1 cut(s) 3720
EcoICRI GAGCTC 1 cut(s) 9
EcoO109I RGGNCCY 1 cut(s) 3902
EcoRI GAATTC 2 cut(s) 3706, 5448
EcoRII CCWGG 8 cut(s) 440, 1218, 2244, 3402, 3552, 3898, 4620, 5063
EcoRV GATATC 1 cut(s) 2974
EcoT14I CCWWGG 8 cut(s) 21, 197, 223, 246, 3330, 4647, 4713, 4755
EcoT22I ATGCAT 2 cut(s) 1672, 2031
EcoT38I GRGCYC 2 cut(s) 11, 1969
ErhI CCWWGG 8 cut(s) 21, 197, 223, 246, 3330, 4647, 4713, 4755
FalI AAGNNNNNCTT 6 cut(s) 1966, 1998, 2426, 2458, 3291, 3323
FaqI GGGAC 4 cut(s) 31, 329, 3498, 5011
FauI CCCGC 1 cut(s) 8
FauNDI CATATG 2 cut(s) 1037, 1615
FbaI TGATCA 2 cut(s) 2532, 3003
FblI GTMKAC 4 cut(s) 716, 2817, 2827, 5410
FriOI GRGCYC 2 cut(s) 11, 1969
GlaI GCGC 6 cut(s) 33, 240, 639, 1940, 3200, 5460
GsaI CCCAGC 2 cut(s) 4634, 5645
GsuI CTGGAG 1 cut(s) 4754
HaeII RGCGCY 2 cut(s) 641, 5462
HapII CCGG 5 cut(s) 166, 428, 4113, 5687, 5790
HgaI GACGC 5 cut(s) 298, 2786, 2936, 3599, 4466
HhaI GCGC 6 cut(s) 34, 241, 640, 1941, 3201, 5461
Hin1I GRCGYC 4 cut(s) 1581, 2778, 4477, 4582
Hin6I GCGC 6 cut(s) 32, 239, 638, 1939, 3199, 5459
HinP1I GCGC 6 cut(s) 32, 239, 638, 1939, 3199, 5459
HincII GTYRAC 2 cut(s) 717, 3762
HindII GTYRAC 2 cut(s) 717, 3762
HindIII AAGCTT 3 cut(s) 1047, 3893, 4017
HpaI GTTAAC 1 cut(s) 3762
HpaII CCGG 5 cut(s) 166, 428, 4113, 5687, 5790
HphI GGTGA 6 cut(s) 1055, 3707, 3872, 3932, 4772, 5169
Hpy99I CGWCG 6 cut(s) 100, 110, 139, 3593, 4065, 4482
HpyCH4IV ACGT 7 cut(s) 268, 1174, 1581, 3764, 3778, 4582, 5036
HpySE526I ACGT 7 cut(s) 268, 1174, 1581, 3764, 3778, 4582, 5036
Hsp92I GRCGYC 4 cut(s) 1581, 2778, 4477, 4582
HspAI GCGC 6 cut(s) 32, 239, 638, 1939, 3199, 5459
KpnI GGTACC 1 cut(s) 494
Ksp22I TGATCA 2 cut(s) 2532, 3003
KspAI GTTAAC 1 cut(s) 3762
LguI GCTCTTC 3 cut(s) 1670, 5641, 5747
MabI ACCWGGT 1 cut(s) 1218
MaeII ACGT 7 cut(s) 268, 1174, 1581, 3764, 3778, 4582, 5036
MaeIII GTNAC 8 cut(s) 1687, 1945, 3257, 3734, 3860, 4165, 4546, 4940
MfeI CAATTG 1 cut(s) 1001
MflI RGATCY 6 cut(s) 250, 1553, 2551, 4617, 4859, 5172
MhlI GDGCHC 8 cut(s) 11, 480, 649, 1969, 3177, 3643, 5382, 5572
MlsI TGGCCA 4 cut(s) 440, 1339, 3012, 4221
MluNI TGGCCA 4 cut(s) 440, 1339, 3012, 4221
MlyI GAGTC 7 cut(s) 300, 1552, 1822, 2227, 2378, 3277, 4998
Mox20I TGGCCA 4 cut(s) 440, 1339, 3012, 4221
Mph1103I ATGCAT 2 cut(s) 1672, 2031
MroXI GAANNNNTTC 3 cut(s) 1799, 3303, 4120
MscI TGGCCA 4 cut(s) 440, 1339, 3012, 4221
MslI CAYNNNNRTG 9 cut(s) 1374, 1607, 2034, 2269, 3170, 3233, 3989, 4718, 4772
Msp20I TGGCCA 4 cut(s) 440, 1339, 3012, 4221
MspA1I CMGCKG 5 cut(s) 15, 4072, 4198, 4780, 4786
MspCI CTTAAG 1 cut(s) 4294
MspI CCGG 5 cut(s) 166, 428, 4113, 5687, 5790
MunI CAATTG 1 cut(s) 1001
Mva1269I GAATGC 9 cut(s) 687, 1079, 1369, 1672, 2031, 2200, 2735, 4503, 5753
MvaI CCWGG 8 cut(s) 442, 1220, 2246, 3404, 3554, 3900, 4622, 5065
MvnI CGCG 1 cut(s) 34
NciI CCSGG 2 cut(s) 167, 5791
NcoI CCATGG 3 cut(s) 223, 246, 4713
NdeI CATATG 2 cut(s) 1037, 1615
NheI GCTAGC 3 cut(s) 2092, 2618, 2914
NlaIV GGNNCC 7 cut(s) 19, 252, 492, 974, 1869, 2314, 5708
NmeAIII GCCGAG 1 cut(s) 2696
NmuCI GTSAC 6 cut(s) 1687, 1945, 3257, 3860, 4165, 4940
NotI GCGGCCGC 1 cut(s) 35
NsiI ATGCAT 2 cut(s) 1672, 2031
NspI RCATGY 3 cut(s) 3238, 3265, 5274
NspV TTCGAA 1 cut(s) 2464
PagI TCATGA 3 cut(s) 3228, 4354, 5487
PceI AGGCCT 1 cut(s) 5766
PciSI GCTCTTC 3 cut(s) 1670, 5641, 5747
PcsI WCGNNNNNNNCGW 2 cut(s) 274, 315
PctI GAATGC 9 cut(s) 687, 1079, 1369, 1672, 2031, 2200, 2735, 4503, 5753
PdmI GAANNNNTTC 3 cut(s) 1799, 3303, 4120
PflMI CCANNNNNTGG 3 cut(s) 1099, 3184, 5565
PfoI TCCNGGA 3 cut(s) 3402, 3552, 4620
PleI GAGTC 7 cut(s) 300, 1551, 1821, 2227, 2377, 3277, 4997
PpsI GAGTC 7 cut(s) 300, 1551, 1821, 2227, 2377, 3277, 4997
Ppu21I YACGTR 2 cut(s) 1175, 3779
PpuMI RGGWCCY 1 cut(s) 3902
PshBI ATTAAT 1 cut(s) 2850
PsiI TTATAA 5 cut(s) 1205, 1842, 2507, 2987, 4346
Psp124BI GAGCTC 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 3902
Psp6I CCWGG 8 cut(s) 440, 1218, 2244, 3402, 3552, 3898, 4620, 5063
PspFI CCCAGC 2 cut(s) 4630, 5641
PspGI CCWGG 8 cut(s) 440, 1218, 2244, 3402, 3552, 3898, 4620, 5063
PspN4I GGNNCC 7 cut(s) 19, 252, 492, 974, 1869, 2314, 5708
PspPI GGNCC 6 cut(s) 18, 1401, 2358, 3406, 3902, 4705
PspPPI RGGWCCY 1 cut(s) 3902
PstI CTGCAG 2 cut(s) 3372, 4785
PstNI CAGNNNCTG 1 cut(s) 1727
PsuI RGATCY 6 cut(s) 250, 1553, 2551, 4617, 4859, 5172
PvuII CAGCTG 2 cut(s) 4780, 4786
RsaI GTAC 9 cut(s) 465, 492, 897, 1173, 1229, 2714, 3521, 4206, 4931
RsaNI GTAC 9 cut(s) 464, 491, 896, 1172, 1228, 2713, 3520, 4205, 4930
RseI CAYNNNNRTG 9 cut(s) 1374, 1607, 2034, 2269, 3170, 3233, 3989, 4718, 4772
SacI GAGCTC 1 cut(s) 11
SalI GTCGAC 1 cut(s) 715
SapI GCTCTTC 3 cut(s) 1670, 5641, 5747
Sau96I GGNCC 6 cut(s) 18, 1401, 2358, 3406, 3902, 4705
SchI GAGTC 7 cut(s) 300, 1552, 1822, 2227, 2378, 3277, 4998
SduI GDGCHC 8 cut(s) 11, 480, 649, 1969, 3177, 3643, 5382, 5572
SexAI ACCWGGT 1 cut(s) 1218
SfuI TTCGAA 1 cut(s) 2464
SinI GGWCC 6 cut(s) 18, 1401, 2358, 3406, 3902, 4705
SmiMI CAYNNNNRTG 9 cut(s) 1374, 1607, 2034, 2269, 3170, 3233, 3989, 4718, 4772
SmlI CTYRAG 3 cut(s) 3749, 4294, 5225
SmoI CTYRAG 3 cut(s) 3749, 4294, 5225
SseBI AGGCCT 1 cut(s) 5766
SspI AATATT 6 cut(s) 1569, 1698, 2209, 2283, 3745, 4086
SstI GAGCTC 1 cut(s) 11
StuI AGGCCT 1 cut(s) 5766
StyI CCWWGG 8 cut(s) 21, 197, 223, 246, 3330, 4647, 4713, 4755
TaiI ACGT 7 cut(s) 271, 1177, 1584, 3767, 3781, 4585, 5039
TatI WGTACW 6 cut(s) 463, 895, 1227, 2712, 3519, 4929
TauI GCSGC 6 cut(s) 37, 40, 149, 219, 2125, 4266
TseFI GTSAC 6 cut(s) 1687, 1945, 3257, 3860, 4165, 4940
Tsp45I GTSAC 6 cut(s) 1687, 1945, 3257, 3860, 4165, 4940
TspGWI ACGGA 8 cut(s) 113, 632, 2325, 2556, 2846, 4851, 5082, 5231
Van91I CCANNNNNTGG 3 cut(s) 1099, 3184, 5565
Vha464I CTTAAG 1 cut(s) 4294
VpaK11BI GGWCC 6 cut(s) 18, 1401, 2358, 3406, 3902, 4705
VspI ATTAAT 1 cut(s) 2850
XbaI TCTAGA 2 cut(s) 3497, 3535
XceI RCATGY 3 cut(s) 3238, 3265, 5274
XcmI CCANNNNNNNNNTGG 1 cut(s) 5050
XmaJI CCTAGG 1 cut(s) 197
XmiI GTMKAC 4 cut(s) 716, 2817, 2827, 5410
XmnI GAANNNNTTC 3 cut(s) 1799, 3303, 4120
ZraI GACGTC 2 cut(s) 1582, 4583
Zsp2I ATGCAT 2 cut(s) 1672, 2031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.