MD17G1137400.v1.1

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0009173
Physical Location & Seq
Forward (+)
12304280 .. 12320779
16500 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1137400.v1.1.491

Sequence Viewer

Length: 5811 bp
ATGGCGAGCTCCAGCGGGACGAAGACCGACCACCCGGGGGGGCTTCAACGGTCGTCATCGAGGGGAATGACGAGAATGTCCACTAAGGTTTTGGACATGCTGATCGAGGACGAGGCGTTGGACATTAATGTGCCCCCGGGACTTCCCTCAATTGCTCCGATTTTTCGAGTCGCCAATGAGATTGAGAAGGACAACCCTAGGGTTGCTTATCTCTGCCGCTTCCATGGATTTGAGAAGGCACATACGATGGATCCAACATCAAGTGGACGTGGTGTTCGACAGTTTAAGACGCTTCTGTTGCACAGACTTGAAAAGGAAGAAACAGAGACAAAACATCAGCTTGCTAGATCTGATGCACAAGAAATTCTGAAGTATTACCACCAATTCTATGAGAAAAATATCAAAGATGGAGAGTACACAAAGAAACCGGAAGAGATGGCTAAGAATTGTCAGATTGCAACAGTACTGTATGATGTGCTCCAGACAGTGGTACATCAATCCCAAATAGATCAACAGACAAAGAAGATGGCTGAAGATGTCAAGAAGAAAAGGGAGCAGTATGTCAACTATAACATTCTTCCGTTATATACTGTTGGTGTTAAACCAGCAATTATGGAACTTCCCGAGATCAAAGCAGCGCTTCGTGCTGTACAAAATGTGAATGGTCTTCCAATGCCAAGATTTCATGTACAACCCACGAACCCTGAAGACAAGTCAACAATGCCAGCAGAGAGGATTAAACCTGTCAATGATATACTTGATTGGCTTTCCTCTATCTTCGGGTTTCAGAAAGGAAATGTGGCAAATCAGAGGGAACACTTACTCTTGCTACTTGCCAACATGGACGTAAGACATAGAAATCTTGAAAATTACACTGAGTTGAATAGTTCCACTGTACAACGTTTGATGGAGAAAGTTTTTAAAAATTATCGTTCCTGGTTTAACTATTTGCATTGTAAATCCAATCTTAGGTTCCCTCGAGGTTCTGACAGACAACAATTGGAACTTATTTACATTGCGCTCTATCTTCTCATATGGGGTGAAGCTTCAAATATTCGATTCATGCCTGAATGCTTATGCTATATTTTCCACCAGATGGCCAATGAGATGTATGGAATTTTATTTAGCAATGTGCATCCGGCTACTGGAGAAACATACCAAGCAGCAGCACGGGATGAAGAGTCTTTTTTGAGGGATGTTGTAACTCCTATCTACCAAGTCTTGTACATGGAAGCAAAGAGAAACAAAAATGGCACGGCAAGTCATTCAAGATGGAGAAATTATGATGATCTAAATGAATACTTCTGGTCCGACAGATGTTTTAGGCTAGGGTGGCCAATGGACCCGAAAGCAGATTTTTTTAAGCAAGATGAGAGGCCAAATCAAGCTGCTGGTGGGAGGAGGAAACCTAAAACAAATTTTGTAGAAGTCCGAACATTTTGGCACCTCTACAGAAGTTTTGATCGAATGTGGATATTCTTGATATTGGCTTTCCAGGCTATGCTTATTGTTGCATGGAGTCCTTCTGGTTCTCTGACTGCTTTCTTTGATGCGGATGTCTTCAGAAGCATTTTAAGCATCTTCATCACTTATGCTTTCCTCAACTTATTACAAGCCACTCTGGATATAATTCTTAGTTGGCATTGTTGGAAGAGCTTGAAATTCACTCAAATACTACGATACCTCTTGAAATTCGCTGTAGCTGGTGCATGGGCTGTGGTTCTGCCTATTGGTTATTCTAGTTCTGTGCAGAATCCTACAGGACTTTTGAAATTCTTCAGCAGTTGGGCTAGGGATTGGCGGAATCAATCATTTTATAATTATGCTGTTGCGCTCTATCTGCTTCCTAATATATTGGCCGCCGTGCTATTTTTTCTTCCACCTTTACGGAGGCACATAGAGCGCTCAAATTGGAGGATTCCAAAACTGTATATCGGAAGAGGGCTTCATGAGGACATCTTTTCACTGCTGAAATATACGCTTTTTTGGATCATGCTTCTAATCAGCAAGCTATCGTTCAGCTATTTTGTGGAGATACTGCCACTTGTAGCACCAACAAAAATAATAATGGAGATGCCTATAAGTAATTATCAATGGCATGAGTTCTTTCCACATGTAACTCATAATATTGGCGTTGTCATTGCAATATGGGCTCCAATTGTCCTGGTTTATTTTATGGATGCACAAATATGGTATGCAATATTTTCTACTATCTTTGGTGGGATTCATGGAGCATTCAGCCATTTGGGTGAGATACGGACCCTTGGGATGTTGAGGTCCAGATTTGAGTCTGTGCCTTCTGCTTTCAGTAATCGTCTTATGCCGTCAGGCAATGAGGATGCAAAGGAGAAAGGACAATTGGTGGATGAGGCACTCGAACGAAAGAACATTGCTAATTTCTCTCAAGTCTGGAATGGGTTCATAAATTCAATGCGGTTGGAAGACCTTATCAGCAATAGAGATAAAGATCTGCTGTTTGTTCCATATTCATCAGAAGATGTGACTGTTGTCCAGTGGCCTCCTTTCTTGCTTGCTAGTAAGATTCCGATAGCATTGGACATGGCAAAAGATTTTACAGGGAAGGCAGATGATGATTTATTTAGAAAGATCAAGAGTGATGATTATATGTACTCAGCAGTAATTGAATGCTATGAGACACTCAGGGATATTATAACTGGCCTTCTGGAAGAGGAAGAGGATAAGATGATTGTAAACCACATATGCAAGGAAGTAGACAGTAGTATACAGCAGCGGAAATTTTTGACTACTTTCCGCATGAGTGGGCTGCCATTTCTTAGCGAGAGGTTGGAGAAATTTCTAAAGCTCTTGCAAGCCGAAGGAGAGAATGTCGATAATTCCATGCGTCAGATAATCAATGTTCTTCAAGATATCATGGAGATTATCACACAGGATGTTATGATCAACGGCCATGCAATACTGGAGCAAGCACATTACACTGATGGTGCAAACGTCAAGAAGGAGCAAAGGTTTCAAAAGCTTAAGATTGACCTTAGACATAATACATCTCGGAGGGAGAAGGCTTGGATAGAAAAGGTAGTGAGACTTCATTTGCTTTTGACCGTCAAAGAGTCTGCCATCAATGTGCCTCAAAACTTGGAAGCTCGCAGGCGCATCACCTTTTTTGCGAACTCCTTATTCATGAACATGCCACGTGCTCCCAAAGTTCGCAGCATGCTCTCATTTAGTGTTCTGACTCCTTATTACAAAGAAGATGTCCTCTACTCTGATGAGGAACTTACGAAAGAAAATGAGGATGGAATCTCAATTTTGTTCTACCTGAAGAAAATATATCCTGATGAATGGACAAATTTTGGGGAACGGATAAATGATCCTAAAAACAAGTTTTCTGAGAACAACAAGTTAGAGTTAACTCGTCAATGGGCATCTTACAGAGCACAGACACTTTCTAGAACAGTGAGAGGGATGATGTACTATAGGGAGGCTCTAGACCTTCAGTGTTTCCTGGAAACAGCAGGAGATGGTGCTGTTTTTGAAGACTACCAAACCTCAGCCTTAAGTGAAAATGACGAGCAGGCTCACCCGGCTTCTGATGATCGTGCACAAGCTCTGGCAGATTTGAAGTTTACTTATGTAGTTTCTTGTCAGGTCTATGGTGCTCAGAAAATCTCCAATGAATCTCGGGAAAAAAGTTGTTACAATAATATCCTGAAGCTGATGCTAACGTATCCATCTCTACGTATTGCTTACATAGATACAACGGAGGAGCAAGTGAAAGGAAAACCCAAAAAAACTTACTATTCTGTTCTAGTCAAGGGTGGTGACAAATGGGATGAGGAAATATACCGCATCAAGCTCCCAGGTCCTCCTACAGATATTGGCGAAGGAAAACCTGAAAATCAAAATCATGCCATTATCTTTACTCGCGGGGAAGCCCTGCAGACAATAGACATGAATCAGGATAATTACTTTGAAGAAGCTTTCAAGATGAGAAATGTGTTGGAGGAATTCTTAAAGAATCGTCGTGGGCATCGAAAACCCACAATATTGGGTCTAAGGGAGCATATTTTCACTGGAAGTGTTTCATCACTTGCTTGGTTCATGTCGAATCAAGAGACCAGTTTTGTAACAATTGGCCAAAGAATTTTGGCAAATCCACTGAGGGTACGGTTCCATTATGGTCATCCTGATATATTTGACAGAATCTTCCACATAACAAGGGGTGGCATAAGCAAAGCTTCAAAAGTAATAAACTTAAGTGAGGATATATTTGCAGGGTACAATTCAACTATGCGTGGGGGATTTATAACGCATCACGAGTATATCCAAGTAGGCAAGGGGCGTGATGTGGGAATGAACCAAATATCACTTTTTGAGGCTAAGGTTTCAAATGGAAATGGAGAACAGACACTTAGCCGTGATGTTTACCGGCTTGGGCGTCGGTTTGATTTCTACAGAATGCTTTCCTTTTACTTCACAACAGTTGGATTCTATTTTAGTAGTATGGTAACTGTGCTGACTGTATATGTGTTTTTATATGGACGTATGTACATGGTTATGAGTGGATTAGAGAATGAGATCCTGGAGAATCCAGCCATACATGAGAACAAAGCCTTTGAAGAGGCTTTGGCTACCCAGTCTGTCTTTCAACTGGGCTTGTTGCTTGTGCTTCCAATGATCATGGAAATTGGCCTAGAGAAAGGGTTCCGCAGTGCCTTGGGTGATCTCATCATCATGCAGCTCCAGTTGGCCTCTGTTTTCTTCACATTCCAACTAGGAACAAAAGCCCATTACTATGGAAGGACAATCTTACACGGAGGATCTAAATACCGAGCAACTGGTCGTGGTTTTGTTGTTTTCCATGCAAAGTTTTCCGAGAACTACAGACTTTACTCGCACAGTCACTTTGTGAAGGGGTTGGAATTGCTTATTCTGCTGATTGTGTATGGAGTCTACGGGCAATCGTATAGGAGTTCAAATCTATATTTTTTCATCACTTTGTCCATGTGGTTTCTGGTTGCTTCCTGGTTGTTTGCTCCTTTCATATTCAATCCGTCCAGCTTTGACTGGCAGAAAACTGTAGATGATTGGACAGATTGGAAGAGGTGGATGGGAAATCGTGGTGGTATTGGGATAGCACCTGACAAAAGTTGGGAATCATGGTGGAATGAAGAACAGGAACACCTCAAACACACAGTTATCCGGGGAAGAATTATTGAGATAATACTTGCGTTGCGGTTCCTTATTTACCAATATGGAGTTGTTTACCACCTTGATATAGCCCATCACAGCAAGAGTTTACTGGTTTATGGACTTTCTTGGATAGTTATGGTAACTGTTCTTCTCGTTTTAAAGATGGTTTCCATGGGTAGACGAAGATTTGGCACCGACTTTCAGCTCATGTTCAGGATTCTGAAAGCGCTTTTGTTTCTTGGTTTCATGTCAGTCATGACTGTCCTATTTGTTGTTTGTGGCCTTACTATTTCAGATCTGTTTGCTGCTATCCTTGCCTTCCTACCCACAGGCTGGGCCCTTCTTGTGATCGGGCAAGCATGCAGGCCAATGTTCAAAGGGCTAGGATTCTGGGAATCAATCATGGAGCTTGGAAGAGCATACGATTACATAATGGGGCTAGTAATCTTCATGCCGATAGCGATTTTGTCATGGTTCCCGTTTGTATCAGAGTTCCAAACACGTTTGCTCTTCAACCAAGCATTCAGCAGAGGCCTGCAGATTTCCATGATTCTTGCAGGGAGGAAAGACAACACGGAGGAGTCTCGGAAAGAGAGACCAGACAAGCCTTCAGCATCTCAAGTTCATACCCGCAATTAG

Protein Analysis

1937

Amino Acids

224.4

Weight (kDa)

8.78

Isoelectric Point (pI)

42.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 50 - 189 3.6e-11 Vta1 like
FKS1_dom1 PF14288 335 - 446 3.7e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 840 - 1028 8.4e-68 Callose synthase 1 domain
Glucan_synthase PF02364 1035 - 1121 1.8e-29 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1124 - 1732 4.5e-205 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1818, 2672, 4286
AasI GACNNNNNNGTC 1 cut(s) 76
AbsI CCTCGAGG 1 cut(s) 978
AccB1I GGYRCC 2 cut(s) 1443, 5363
AccB7I CCANNNNNTGG 3 cut(s) 487, 1096, 5505
AccI GTMKAC 4 cut(s) 2733, 2743, 4932, 5350
AccII CGCG 1 cut(s) 3906
AclI AACGTT 1 cut(s) 901
AclWI GGATC 6 cut(s) 245, 258, 1997, 3344, 4552, 4807
AcoI YGGCCR 5 cut(s) 1098, 1334, 1857, 2926, 4114
AcuI CTGAAG 9 cut(s) 389, 552, 726, 1546, 1762, 3320, 3458, 3710, 5766
AcvI CACGTG 1 cut(s) 3173
AcyI GRCGYC 1 cut(s) 4417
AdeI CACNNNGTG 1 cut(s) 4888
AfeI AGCGCT 3 cut(s) 639, 1904, 5400
AfiI CCNNNNNNNGG 6 cut(s) 37, 487, 969, 1096, 1145, 5505
AflII CTTAAG 3 cut(s) 2999, 3535, 4234
AflIII ACRYGT 2 cut(s) 2113, 5672
AjiI CACGTC 1 cut(s) 269
AjnI CCWGG 7 cut(s) 935, 1494, 2163, 3483, 3838, 4560, 5003
AjuI GAANNNNNNNTTGG 4 cut(s) 956, 988, 2342, 2374
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
Alw21I GWGCWC 6 cut(s) 11, 480, 3178, 3418, 3583, 3640
Alw26I GTCTC 6 cut(s) 320, 2648, 3055, 4088, 5760, 5761
Alw44I GTGCAC 1 cut(s) 3579
AlwI GGATC 6 cut(s) 245, 258, 1997, 3344, 4552, 4807
Ama87I CYCGRG 5 cut(s) 34, 136, 623, 978, 3660
Aor51HI AGCGCT 3 cut(s) 639, 1904, 5400
ApaI GGGCCC 1 cut(s) 5512
ApaLI GTGCAC 1 cut(s) 3579
ApeKI GCWGC 9 cut(s) 635, 1163, 1166, 1388, 2749, 2785, 3189, 4717, 5477
ArsI GACNNNNNNTTYG 4 cut(s) 258, 290, 3219, 3251
AseI ATTAAT 1 cut(s) 126
Asp700I GAANNNNTTC 5 cut(s) 1775, 2417, 4060, 4441, 4682
AspA2I CCTAGG 1 cut(s) 197
AspLEI GCGC 6 cut(s) 640, 1021, 1834, 1905, 3132, 5401
AspS9I GGNCC 7 cut(s) 1308, 1342, 2259, 2277, 3842, 5508, 5509
AsuC2I CCSGG 6 cut(s) 35, 36, 137, 138, 3563, 5183
AsuHPI GGTGA 6 cut(s) 1052, 2261, 3127, 3551, 3812, 4712
AvaI CYCGRG 5 cut(s) 34, 136, 623, 978, 3660
AvaII GGWCC 5 cut(s) 1308, 1342, 2259, 2277, 3842
AvrII CCTAGG 1 cut(s) 197
BaeGI GKGCMC 3 cut(s) 135, 3583, 5512
BalI TGGCCA 3 cut(s) 1100, 1336, 4116
BamHI GGATCC 1 cut(s) 250
BanI GGYRCC 2 cut(s) 1443, 5363
BanII GRGCYC 3 cut(s) 11, 2155, 5512
BarI GAAGNNNNNNTAC 4 cut(s) 3048, 3080, 5304, 5336
BauI CACGAG 1 cut(s) 4295
BbrPI CACGTG 1 cut(s) 3173
BbsI GAAGAC 6 cut(s) 29, 659, 714, 1552, 2448, 3522
Bbv12I GWGCWC 6 cut(s) 11, 480, 3178, 3418, 3583, 3640
BbvCI CCTCAGC 1 cut(s) 3529
BbvI GCAGC 9 cut(s) 647, 1175, 1178, 1375, 2761, 2772, 3201, 4729, 5464
BceAI ACGGC 5 cut(s) 1272, 1847, 2308, 2941, 4380
BciT130I CCWGG 7 cut(s) 937, 1496, 2165, 3485, 3840, 4562, 5005
BciVI GTATCC 1 cut(s) 3717
BclI TGATCA 2 cut(s) 2919, 4656
BcnI CCSGG 6 cut(s) 35, 36, 137, 138, 3563, 5183
BcoDI GTCTC 6 cut(s) 320, 2648, 3055, 4088, 5760, 5761
BfoI RGCGCY 3 cut(s) 641, 1906, 5402
BfrI CTTAAG 3 cut(s) 2999, 3535, 4234
BfuI GTATCC 1 cut(s) 3717
BglII AGATCT 3 cut(s) 347, 2467, 5467
BlnI CCTAGG 1 cut(s) 197
BmcAI AGTACT 1 cut(s) 465
Bme18I GGWCC 5 cut(s) 1308, 1342, 2259, 2277, 3842
BmeT110I CYCGRG 5 cut(s) 34, 136, 623, 978, 3660
BmgBI CACGTC 1 cut(s) 269
BmgT120I GGNCC 7 cut(s) 1308, 1342, 2259, 2277, 3842, 5508, 5509
BmrI ACTGGG 2 cut(s) 4609, 4640
BmuI ACTGGG 2 cut(s) 4609, 4640
BoxI GACNNNNGTC 1 cut(s) 2507
BpiI GAAGAC 6 cut(s) 29, 659, 714, 1552, 2448, 3522
BpmI CTGGAG 5 cut(s) 464, 1167, 2960, 4583, 4706
Bpu10I CCTNAGC 2 cut(s) 3529, 4359
BpuEI CTTGAG 2 cut(s) 2388, 5775
BpuMI CCSGG 6 cut(s) 35, 36, 137, 138, 3563, 5183
BsaAI YACGTR 2 cut(s) 3173, 3719
BsaBI GATNNNNATC 1 cut(s) 2466
BsaHI GRCGYC 1 cut(s) 4417
BsaI GGTCTC 2 cut(s) 4088, 5761
BsaWI WCCGGW 1 cut(s) 427
BsaXI ACNNNNNCTCC 4 cut(s) 2832, 2862, 2972, 3002
Bsc4I CCNNNNNNNGG 6 cut(s) 37, 487, 969, 1096, 1145, 5505
Bse118I RCCGGY 1 cut(s) 4407
Bse3DI GCAATG 5 cut(s) 1014, 1135, 2139, 2338, 2388
Bse8I GATNNNNATC 1 cut(s) 2466
BseBI CCWGG 7 cut(s) 937, 1496, 2165, 3485, 3840, 4562, 5005
BseJI GATNNNNATC 1 cut(s) 2466
BseLI CCNNNNNNNGG 6 cut(s) 37, 487, 969, 1096, 1145, 5505
BseMI GCAATG 5 cut(s) 1014, 1135, 2139, 2338, 2388
BseMII CTCAG 7 cut(s) 867, 2646, 2674, 3360, 3543, 3653, 4130
BseRI GAGGAG 3 cut(s) 1414, 3758, 5765
BseSI GKGCMC 3 cut(s) 135, 3583, 5512
BseXI GCAGC 9 cut(s) 647, 1175, 1178, 1375, 2761, 2772, 3201, 4729, 5464
BseYI CCCAGC 1 cut(s) 5505
BsgI GTGCAG 1 cut(s) 1769
Bsh1236I CGCG 1 cut(s) 3906
Bsh1285I CGRYCG 1 cut(s) 53
BshNI GGYRCC 2 cut(s) 1443, 5363
BsiEI CGRYCG 1 cut(s) 53
BsiHKAI GWGCWC 6 cut(s) 11, 480, 3178, 3418, 3583, 3640
BsiHKCI CYCGRG 5 cut(s) 34, 136, 623, 978, 3660
BsiSI CCGG 7 cut(s) 35, 137, 428, 1139, 3563, 4408, 5182
BslFI GGGAC 2 cut(s) 31, 153
BslI CCNNNNNNNGG 6 cut(s) 37, 487, 969, 1096, 1145, 5505
BsmAI GTCTC 6 cut(s) 320, 2648, 3055, 4088, 5760, 5761
BsmFI GGGAC 2 cut(s) 31, 153
BsmI GAATGC 5 cut(s) 1076, 2234, 2651, 4443, 5693
Bso31I GGTCTC 2 cut(s) 4088, 5761
BsoBI CYCGRG 5 cut(s) 34, 136, 623, 978, 3660
Bsp120I GGGCCC 1 cut(s) 5508
Bsp1286I GDGCHC 9 cut(s) 11, 135, 480, 2155, 3178, 3418, 3583, 3640, 5512
Bsp1407I TGTACA 5 cut(s) 649, 688, 895, 1224, 4527
Bsp19I CCATGG 2 cut(s) 223, 5343
BspCNI CTCAG 7 cut(s) 868, 2645, 2673, 3361, 3542, 3652, 4131
BspFNI CGCG 1 cut(s) 3906
BspHI TCATGA 3 cut(s) 1948, 3159, 5427
BspMAI CTGCAG 2 cut(s) 3921, 5712
BspPI GGATC 6 cut(s) 245, 258, 1997, 3344, 4552, 4807
BspQI GCTCTTC 3 cut(s) 1646, 5581, 5687
BspT107I GGYRCC 2 cut(s) 1443, 5363
BspTI CTTAAG 3 cut(s) 2999, 3535, 4234
BspTNI GGTCTC 2 cut(s) 4088, 5761
BsrDI GCAATG 5 cut(s) 1014, 1135, 2139, 2338, 2388
BsrFI RCCGGY 1 cut(s) 4407
BsrGI TGTACA 5 cut(s) 649, 688, 895, 1224, 4527
BssAI RCCGGY 1 cut(s) 4407
BssNAI GTATAC 1 cut(s) 2744
BssNI GRCGYC 1 cut(s) 4417
BssSI CACGAG 1 cut(s) 4295
BssT1I CCWWGG 5 cut(s) 197, 223, 2263, 4695, 5343
Bst1107I GTATAC 1 cut(s) 2744
Bst2BI CACGAG 1 cut(s) 4295
Bst2UI CCWGG 7 cut(s) 937, 1496, 2165, 3485, 3840, 4562, 5005
BstACI GRCGYC 1 cut(s) 4417
BstAFI CTTAAG 3 cut(s) 2999, 3535, 4234
BstAUI TGTACA 5 cut(s) 649, 688, 895, 1224, 4527
BstBAI YACGTR 2 cut(s) 3173, 3719
BstDSI CCRYGG 2 cut(s) 223, 5343
BstFNI CGCG 1 cut(s) 3906
BstH2I RGCGCY 3 cut(s) 641, 1906, 5402
BstHHI GCGC 6 cut(s) 640, 1021, 1834, 1905, 3132, 5401
BstMAI GTCTC 6 cut(s) 320, 2648, 3055, 4088, 5760, 5761
BstMCI CGRYCG 1 cut(s) 53
BstNI CCWGG 7 cut(s) 937, 1496, 2165, 3485, 3840, 4562, 5005
BstNSI RCATGY 5 cut(s) 100, 2117, 3169, 3196, 5535
BstPAI GACNNNNGTC 1 cut(s) 2507
BstSLI GKGCMC 3 cut(s) 135, 3583, 5512
BstSNI TACGTA 1 cut(s) 3719
BstUI CGCG 1 cut(s) 3906
BstV1I GCAGC 9 cut(s) 647, 1175, 1178, 1375, 2761, 2772, 3201, 4729, 5464
BstV2I GAAGAC 6 cut(s) 29, 659, 714, 1552, 2448, 3522
BstX2I RGATCY 6 cut(s) 250, 347, 2467, 4557, 4799, 5467
BstXI CCANNNNNNTGG 2 cut(s) 4027, 4775
BstYI RGATCY 6 cut(s) 250, 347, 2467, 4557, 4799, 5467
BstZ17I GTATAC 1 cut(s) 2744
BsuI GTATCC 1 cut(s) 3717
BtgI CCRYGG 2 cut(s) 223, 5343
BtrI CACGTC 1 cut(s) 269
BtsI GCAGTG 2 cut(s) 1964, 4696
CciI TCATGA 3 cut(s) 1948, 3159, 5427
CfoI GCGC 6 cut(s) 640, 1021, 1834, 1905, 3132, 5401
Cfr10I RCCGGY 1 cut(s) 4407
Cfr13I GGNCC 7 cut(s) 1308, 1342, 2259, 2277, 3842, 5508, 5509
Cfr9I CCCGGG 2 cut(s) 34, 136
CseI GACGC 3 cut(s) 298, 2852, 4406
DraI TTTAAA 2 cut(s) 922, 5331
DraIII CACNNNGTG 1 cut(s) 4888
DrdI GACNNNNNNGTC 1 cut(s) 76
DseDI GACNNNNNNGTC 1 cut(s) 76
EaeI YGGCCR 5 cut(s) 1098, 1334, 1857, 2926, 4114
EciI GGCGGA 1 cut(s) 1816
Ecl136II GAGCTC 1 cut(s) 9
Eco105I TACGTA 1 cut(s) 3719
Eco130I CCWWGG 5 cut(s) 197, 223, 2263, 4695, 5343
Eco147I AGGCCT 1 cut(s) 5706
Eco24I GRGCYC 3 cut(s) 11, 2155, 5512
Eco31I GGTCTC 2 cut(s) 4088, 5761
Eco32I GATATC 1 cut(s) 2890
Eco47I GGWCC 5 cut(s) 1308, 1342, 2259, 2277, 3842
Eco47III AGCGCT 3 cut(s) 639, 1904, 5400
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 9 cut(s) 389, 552, 726, 1546, 1762, 3320, 3458, 3710, 5766
Eco72I CACGTG 1 cut(s) 3173
Eco88I CYCGRG 5 cut(s) 34, 136, 623, 978, 3660
EcoICRI GAGCTC 1 cut(s) 9
EcoO109I RGGNCCY 2 cut(s) 3842, 5509
EcoRI GAATTC 1 cut(s) 3986
EcoRII CCWGG 7 cut(s) 935, 1494, 2163, 3483, 3838, 4560, 5003
EcoRV GATATC 1 cut(s) 2890
EcoT14I CCWWGG 5 cut(s) 197, 223, 2263, 4695, 5343
EcoT38I GRGCYC 3 cut(s) 11, 2155, 5512
ErhI CCWWGG 5 cut(s) 197, 223, 2263, 4695, 5343
FalI AAGNNNNNCTT 4 cut(s) 624, 656, 4201, 4233
FaqI GGGAC 2 cut(s) 31, 153
FauI CCCGC 2 cut(s) 8, 3899
FauNDI CATATG 2 cut(s) 1034, 2720
FbaI TGATCA 2 cut(s) 2919, 4656
FblI GTMKAC 4 cut(s) 2733, 2743, 4932, 5350
FriOI GRGCYC 3 cut(s) 11, 2155, 5512
GlaI GCGC 6 cut(s) 639, 1020, 1833, 1904, 3131, 5400
GsaI CCCAGC 1 cut(s) 5509
GsuI CTGGAG 5 cut(s) 464, 1167, 2960, 4583, 4706
HaeII RGCGCY 3 cut(s) 641, 1906, 5402
HapII CCGG 7 cut(s) 35, 137, 428, 1139, 3563, 4408, 5182
HgaI GACGC 3 cut(s) 298, 2852, 4406
HhaI GCGC 6 cut(s) 640, 1021, 1834, 1905, 3132, 5401
Hin1I GRCGYC 1 cut(s) 4417
Hin6I GCGC 6 cut(s) 638, 1019, 1832, 1903, 3130, 5399
HinP1I GCGC 6 cut(s) 638, 1019, 1832, 1903, 3130, 5399
HincII GTYRAC 3 cut(s) 565, 717, 3390
HindII GTYRAC 3 cut(s) 565, 717, 3390
HindIII AAGCTT 4 cut(s) 1044, 2996, 3957, 4215
HpaI GTTAAC 1 cut(s) 3390
HpaII CCGG 7 cut(s) 35, 137, 428, 1139, 3563, 4408, 5182
HphI GGTGA 6 cut(s) 1052, 2261, 3127, 3551, 3812, 4712
Hpy99I CGWCG 2 cut(s) 4005, 4422
HpyCH4IV ACGT 9 cut(s) 268, 846, 901, 2970, 3172, 3704, 3718, 4522, 5674
HpySE526I ACGT 9 cut(s) 268, 846, 901, 2970, 3172, 3704, 3718, 4522, 5674
Hsp92I GRCGYC 1 cut(s) 4417
HspAI GCGC 6 cut(s) 638, 1019, 1832, 1903, 3130, 5399
Ksp22I TGATCA 2 cut(s) 2919, 4656
KspAI GTTAAC 1 cut(s) 3390
LguI GCTCTTC 3 cut(s) 1646, 5581, 5687
Lsp1109I GCAGC 9 cut(s) 647, 1175, 1178, 1375, 2761, 2772, 3201, 4729, 5464
MaeII ACGT 9 cut(s) 268, 846, 901, 2970, 3172, 3704, 3718, 4522, 5674
MaeIII GTNAC 9 cut(s) 1201, 2116, 2500, 3674, 3800, 4105, 4486, 4880, 5311
MfeI CAATTG 5 cut(s) 150, 998, 2157, 2357, 4110
MflI RGATCY 6 cut(s) 250, 347, 2467, 4557, 4799, 5467
MhlI GDGCHC 9 cut(s) 11, 135, 480, 2155, 3178, 3418, 3583, 3640, 5512
MlsI TGGCCA 3 cut(s) 1100, 1336, 4116
MluNI TGGCCA 3 cut(s) 1100, 1336, 4116
MlyI GAGTC 8 cut(s) 177, 1190, 1528, 2297, 3098, 3208, 4938, 5762
Mox20I TGGCCA 3 cut(s) 1100, 1336, 4116
MroXI GAANNNNTTC 5 cut(s) 1775, 2417, 4060, 4441, 4682
MscI TGGCCA 3 cut(s) 1100, 1336, 4116
MslI CAYNNNNRTG 8 cut(s) 128, 2188, 2247, 3101, 3164, 4535, 4712, 4773
Msp20I TGGCCA 3 cut(s) 1100, 1336, 4116
MspA1I CMGCKG 2 cut(s) 15, 2752
MspCI CTTAAG 3 cut(s) 2999, 3535, 4234
MspI CCGG 7 cut(s) 35, 137, 428, 1139, 3563, 4408, 5182
MunI CAATTG 5 cut(s) 150, 998, 2157, 2357, 4110
Mva1269I GAATGC 5 cut(s) 1076, 2234, 2651, 4443, 5693
MvaI CCWGG 7 cut(s) 937, 1496, 2165, 3485, 3840, 4562, 5005
MvnI CGCG 1 cut(s) 3906
NciI CCSGG 6 cut(s) 35, 36, 137, 138, 3563, 5183
NcoI CCATGG 2 cut(s) 223, 5343
NdeI CATATG 2 cut(s) 1034, 2720
NmuCI GTSAC 3 cut(s) 2500, 3800, 4880
NspI RCATGY 5 cut(s) 100, 2117, 3169, 3196, 5535
PaeI GCATGC 2 cut(s) 3196, 5535
PaeR7I CTCGAG 1 cut(s) 978
PagI TCATGA 3 cut(s) 1948, 3159, 5427
PceI AGGCCT 1 cut(s) 5706
PciI ACATGT 1 cut(s) 2113
PciSI GCTCTTC 3 cut(s) 1646, 5581, 5687
PcsI WCGNNNNNNNCGW 2 cut(s) 274, 4009
PctI GAATGC 5 cut(s) 1076, 2234, 2651, 4443, 5693
PdmI GAANNNNTTC 5 cut(s) 1775, 2417, 4060, 4441, 4682
PflMI CCANNNNNTGG 3 cut(s) 487, 1096, 5505
PfoI TCCNGGA 2 cut(s) 3483, 4560
PleI GAGTC 8 cut(s) 176, 1189, 1527, 2296, 3097, 3208, 4937, 5761
PmaCI CACGTG 1 cut(s) 3173
PmlI CACGTG 1 cut(s) 3173
PpsI GAGTC 8 cut(s) 176, 1189, 1527, 2296, 3097, 3208, 4937, 5761
Ppu21I YACGTR 2 cut(s) 3173, 3719
PpuMI RGGWCCY 1 cut(s) 3842
PscI ACATGT 1 cut(s) 2113
PshAI GACNNNNGTC 1 cut(s) 2507
PshBI ATTAAT 1 cut(s) 126
PsiI TTATAA 3 cut(s) 1818, 2672, 4286
Psp124BI GAGCTC 1 cut(s) 11
Psp1406I AACGTT 1 cut(s) 901
Psp5II RGGWCCY 1 cut(s) 3842
Psp6I CCWGG 7 cut(s) 935, 1494, 2163, 3483, 3838, 4560, 5003
PspCI CACGTG 1 cut(s) 3173
PspFI CCCAGC 1 cut(s) 5505
PspGI CCWGG 7 cut(s) 935, 1494, 2163, 3483, 3838, 4560, 5003
PspOMI GGGCCC 1 cut(s) 5508
PspPI GGNCC 7 cut(s) 1308, 1342, 2259, 2277, 3842, 5508, 5509
PspPPI RGGWCCY 1 cut(s) 3842
PspXI VCTCGAGB 1 cut(s) 978
PstI CTGCAG 2 cut(s) 3921, 5712
PsuI RGATCY 6 cut(s) 250, 347, 2467, 4557, 4799, 5467
RseI CAYNNNNRTG 8 cut(s) 128, 2188, 2247, 3101, 3164, 4535, 4712, 4773
SacI GAGCTC 1 cut(s) 11
SapI GCTCTTC 3 cut(s) 1646, 5581, 5687
Sau96I GGNCC 7 cut(s) 1308, 1342, 2259, 2277, 3842, 5508, 5509
ScaI AGTACT 1 cut(s) 465
SchI GAGTC 8 cut(s) 177, 1190, 1528, 2297, 3098, 3208, 4938, 5762
SduI GDGCHC 9 cut(s) 11, 135, 480, 2155, 3178, 3418, 3583, 3640, 5512
Sfr274I CTCGAG 1 cut(s) 978
SinI GGWCC 5 cut(s) 1308, 1342, 2259, 2277, 3842
SlaI CTCGAG 1 cut(s) 978
SmaI CCCGGG 2 cut(s) 36, 138
SmiMI CAYNNNNRTG 8 cut(s) 128, 2188, 2247, 3101, 3164, 4535, 4712, 4773
SmlI CTYRAG 6 cut(s) 978, 2403, 2999, 3535, 4234, 5790
SmoI CTYRAG 6 cut(s) 978, 2403, 2999, 3535, 4234, 5790
SnaBI TACGTA 1 cut(s) 3719
SphI GCATGC 2 cut(s) 3196, 5535
SseBI AGGCCT 1 cut(s) 5706
SspI AATATT 4 cut(s) 1054, 2128, 2202, 4026
SstI GAGCTC 1 cut(s) 11
StuI AGGCCT 1 cut(s) 5706
StyI CCWWGG 5 cut(s) 197, 223, 2263, 4695, 5343
TaiI ACGT 9 cut(s) 271, 849, 904, 2973, 3175, 3707, 3721, 4525, 5677
TaqII GACCGA 1 cut(s) 41
TatI WGTACW 9 cut(s) 414, 463, 649, 688, 895, 1224, 2628, 3450, 4527
TauI GCSGC 2 cut(s) 219, 1862
TseFI GTSAC 3 cut(s) 2500, 3800, 4880
TseI GCWGC 9 cut(s) 635, 1163, 1166, 1388, 2749, 2785, 3189, 4717, 5477
Tsp45I GTSAC 3 cut(s) 2500, 3800, 4880
TspGWI ACGGA 8 cut(s) 570, 1903, 2272, 3355, 3755, 4809, 5022, 5762
TspMI CCCGGG 2 cut(s) 34, 136
Van91I CCANNNNNTGG 3 cut(s) 487, 1096, 5505
Vha464I CTTAAG 3 cut(s) 2999, 3535, 4234
VneI GTGCAC 1 cut(s) 3579
VpaK11BI GGWCC 5 cut(s) 1308, 1342, 2259, 2277, 3842
VspI ATTAAT 1 cut(s) 126
XbaI TCTAGA 2 cut(s) 3428, 3466
XceI RCATGY 5 cut(s) 100, 2117, 3169, 3196, 5535
XcmI CCANNNNNNNNNTGG 2 cut(s) 88, 4990
XhoI CTCGAG 1 cut(s) 978
XmaI CCCGGG 2 cut(s) 34, 136
XmaJI CCTAGG 1 cut(s) 197
XmiI GTMKAC 4 cut(s) 2733, 2743, 4932, 5350
XmnI GAANNNNTTC 5 cut(s) 1775, 2417, 4060, 4441, 4682
ZrmI AGTACT 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.