Rh2BG502000

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0009173
Physical Location & Seq
Reverse (-)
70806679 .. 70822417
15739 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG502000.1

Sequence Viewer

Length: 5121 bp
ATGGCGAGCTCCAGCGGGACCAAGGCCGATGCGCGGCCGCAGAGGTCGCTGTCGAGGGGAATGACGAGAATGGCTACTAGAGTTGTGGACTTTCCGACGGAGGGCGACGATGCGTTGGACAGTGGAGTTGTGCCGTCGTGCCTTGCGGCTATTGCTCCGATTTTCCGGGTTGCCAATGAGATTGAGAAGGACAACCCTAGGGTTGCTTATCTCTGCCGCTTCCATGGATTTGAAAAGGCGCATACCATGGATCCAACATCAAGTGGACGTGGAGTTCGTCAGTTTAAGACGCATCTTTTGCACAGACTCGAAAGGGACGAAGTAGATACAAAACATCAGCTTGCTAAATCTGATACAAAAGAAATCATGTTGTATTACCACCAATTCTATGAGAAAAATATCAGAGATGGAGAATATACAAAGAAACCGGAAGAGATGGCCAGGATTTGTCAGATTGCAACTGTACTGTATGATGTGCTCAAGACAGTGGTACCTGCTTCTCAAATAGACATACAGACCCAGAGAATTGCTGAAGATGTCAAGAGCAAAAGGGAGCAATATGTAAACTATAACATTCTTCCATTATATACTGTCGGGGTTAAACCGACAATTATGGAACTTCCTGAGATCAAAGCAGCGCTCCGTGCTCTACAGAATGTGAGTAATCTTCCAATGCCAAGAATGCATTTTAAACCCACGAATCCTGATGACAAGTCGACAATGCCTACAGAGAGGATTAAACCTGTGAATGACATACTCGATTGGCTTTCGGCTATTTTTGGGTTTCAGAAAGGAAATGTGGCAAATCAGAGGGAACATTTAATTTTGCTGCTTGCCAACATTGACGTAAGACATAGAAGTCCTGAGAACTACAATGAGTTGAATAGTGCAACTGTACAGCATTTGATGGAGAAATTCTTTAAGAATTATCGTTCATGGTATAACTATCTGCATTGTAAAACAAATCTTCGGTTCCCCGAAGGTTCTCACAGCAAACAACAATTGGAACTTATATACATTGCTCTCTATCTTCTCATATGGGGTGAAGCTTCAAATGTCCGATTCATGCCTGAATGCTTATGCTACATTTTCCATAATATGGCGAACGAGGTCTATGGAATTTTAAATAGCAATGCTCATACTGTTAGTGGAGAAACATACCAAACAGTTGTACGTGATGAAGAATCTTTTTTGAGGGATGTTATAACTCCTATCTACCAGGTTTTGTACAAGGAAGCAAAGAGAAACAAAATGGGCAAGGCAAGTCATTCAAGATGGAGAAATTATGATGATTTGAATGAATACTTCTGGTCGGACAAATGTTTTAAGCTAGGGTGGCCAATGGATGCTAAAGCAGATTTCTTTAGGCATTCAGATGGGATACAACCAGTAAATGAGAGGACCAATCAAGCTACTGGTGGTAGGAGGAAGCCTAAAACAAATTTTGTAGAAGTCCGAACATTTTTACACCTCTACAGAAGTTTTGATCGAATGTGGATATTCTTCATATTGGCTTTACAGGCTATGATTATTATTGCATGGAGTCCTTCTGGATCTATCACTTCAATATTTGATGCAGACGTCTTCAGAAGTGTTTTAAGCATCTTCATGTCATATGCTTTCCTTAATTTATTACAAGCCACTTTGGACATAATTCTCAGTTGGAATGCATGGAAGAGCTTGAAAGTCACTCAAATATTACGATACCTCTTGAAATTTGCAGTAGCTGTTGTCTGGGCTGTGGTTCTGCCTATTGGTTATTCTAGTTCTATGCAAAATCCTACAGGGCTTCTGAAATTCTTCAGTAGTTGGAGTCGGGATTGGCGGAATCAGTCATTTTATAATTATGCTGTTGCAATCTATATGGTTCCTAATTTATTGGCTGCTATGCTGTTTTTTCTTCCACCTTTACGAAGACACATAGAGCGTTCCAACTGGCGCATTGTCACTCTTTTTATGTGGTGGGCTCAGCCAAAACTGTATGTCGGAAGAGGAATGCATGAGGATGTCTTCTCACTGCTGAAATATACATTGTTCTGGATCATGCTGCTAATCAGCAAGCTAGCGTTCAGCTATTATGTGGAGATACTGCCGCTTGTTGCACCAACAAAGTTGATAATGGATATGCCTATCAGTAATTACCAATGGCATGAGTTCTTTCCGAATGCAACTCATAATATTGGTGTTGTCATTGCAATATGGACTCCAATTATCCTGGTATATTTTATGGATGCACAAATATGGTATGCAATATTTTCTACTCTGTTTGGTGGGATTCACGGAGCCTTCAGCCATTTGGGGGAGATACGAACACTTGGGATGTTGCGGTCCAGATTTGAGTCTGTGCCTTCAGCTTTCAGTGATCGGCTTATGCCATCGTCAGAGAATAAGGATGCCAGAAAGAAGAAACACTTGGATGAAGGACTTGTTCGAAAGAACATTGCTAATTTCTCTCAGGTCTGGAATGAATTTATAAATTCTATGCGAATGGAAGACCTGATCAGCAATAGGGATAGGGATCTGCTGCTTGTTCCGTATTCTTCAAGTGATGTTTCTGTTGTCCAGTGGCCTCCTTTCTTGCTTGCTAGCAAGATTCCAATAGCATTAGACATGGCAAAGGATTTTACAGGAAAGTCGGATGATGATTTATTCAGGAAGATTAGGAATGATGATTATATGTACTCGGCAGTGATTGAATGCTATGAGACACTCAGGGATATTATATTTGGCCTTCTTGATGATGAAGCCGATAAGATGATTGTAAGGCAGATATGTTATGAAGTAGACAGTAGTATACAGCAGCAGAGGTTTTTGATTAATTTCCGTATGAGTGGGCTGCCATTTCTTAGTGAGAGGTTGGAGAAGTTTCTCAAACTCTTGCTAGCCGAAGATGAGAATGTCGAAAATTCCTTGCGTCAGATAATCAATGTCCTTCAAGATATCATGGAGATTATAACACAGGATGTAATGATCAATGGCCATGAAATCCTCGAAGCAGCTCACTACATAGAGGGTCAAAATGTCAAGAAGGAGCAAAGGTTTCAAAAAATCAACATATATCTTGCCCAAAATCCATCTTGGAGAGAGAAGGTCGTTAGGCTTCATTTGCTTTTGACCGTCAAGGAATCTGCCATCAATGTGCCCCAAAACTTGGATGCTCGCAGGCGCATTACTTTCTTTGCGAACTCCTTATTCATGAACATGCCAAATGCTCCCAAAGTTCGTGACATGCTCTCCTTTAGTGTTCTGACTCCTTATTACAAAGAAGATGTTCTTTATTCTGATGAGGAACTTACCAAGGAAAATGAGGATGGAATCTCAATTTTGTTCTACCTGCAGAAGATATATCCTGATGAATGGACAAATTTCCTGGACCGCATAAATGAACATAAAAGTCAGTACACTGAAAAGGACAAGTCGGAGTTAATTCGTCAGTGGGTATCTTACAGGGGACAGACACTTTCTAGAACAGTGAGAGGGATGATGTACTATAGGAAGGCTCTAGACCTTCAATGTATCCTGGAAACAGCAGGAGATGATGGTGTTTATGGAGGCAACGCAGCCCATATTTTAAGTGATAAAGAGGAGAAGGATATTCTTGATCGGGCACAAGCTCTGGCAGATTTGAAGTTCACTTATGTAGTTTCTTGTCAGGTCTATGGTGAACAGAAGAATTCCACTGAAGCCCGAGACAAAAGTTGTTACACTAATATTCTCAAGCTGATGTTAACGTATCCATCTCTACGTGTTGCTTACATAGATACAAGAGAGAAGACAGTGAATGGCAAATCCCAAAAGATTCATTTCTCTGTTCTGGTCAAGGGAGGTGACAAATGGGATGAGGTAACTGTGCTGACTGTCTATGTGTTTTTATATGGACGTCTATATATGGTTATGAGTGGATTGGAAAGTGAGATCCTGGATAGCCCAGCCATACATCAGACCAAGGCTTTTGAACAAGCTCTGGCTACACAGTCTGTCTTTCAACTGGGCTTGTTGCTGGTCCTTCCCATGGTTATGGAAATTGGTCTGGAGAAAGGATTTCGCACTGCCTTGGGTGATTTCATCATCATGCAGCTGCAGCTGGCCTCTGTTTTCTTCACTTTCCAACTAGGAACAAAAGCACATTACTACGGAAGGACAATCTTACATGGAGGATCTAAATATCGAGCAACTGGTCGTGGTTTTGTTGTTTTTCATGCAAAGTTTGCCGATAATTACAGGTTGTACTCACGAAGTCACTTTGTGAAAGGGTTGGAGCTGTTGATTCTGCTGATTGTGTATGGAGTCTATGGGACTGCATATAGGAGTTCAAATCTGTATTTTTTCATCACGTTCTCCATGTGGTTTCTGGTTGCTTCCTGGTTGTTTGCTCCTTTTATCTTTAATCCGTCCAGCTTTGACTGGCAAAAAACTGTAGATGATTGGACGGATTGGAAGAGGTGGATGGGAAATCGTGGTGGGATTGGGATCTCACCAGACAAAAGTTGGGAATCATGGTGGGATGAAGAACAGGAACACCTCAAGCACACTATTATCCGTGGAAGAATTATTGAGATAATTCTTGCATGTCGCTTCTTTTTTTACCAATATGGGATTGTTTACCACCTCGATATAGCTCATTTCAGCAAGAGTTTACTGGTTTATGGACTTTCTTGGGTGGTTATGATAACGGTGCTCCTTGTCTTAAAGATGGTGTCAATGGGTAGACGAAGATTTGGCACTGACTTTCAGCTCATGTTCAGAATTCTGAAGGCGCTTCTTTTTCTTGGCTTCATGTCAGTCATGACTGTCCTATTTGTAGTTTGTGGCCTTACTATTTCTGATCTATTTGCTGCTATTCTTGCCTTCTTGCCCACAGGGTGGGCACTACTTCTTATCGGGCAAGTGTGCAGGCCAATGGTTAAGGGAGTAGGGTTCTGGGAATCAATTAAGGAGCTGGGAAGAGCCTATGATTACATAATGGGAGTGGTAGTCTTCATGCCGGTTGCCATTTTGTCATGGTTCCCATTTGTCTCAGAGTTCCAAACACGGTTGCTCTTCAACCAAGCATTCAGTCGAGGCCTACAAATTTCAATGATTCTCGCCGGGAGGAAAGATAAGACAGCTTCTAATAGATAG

Protein Analysis

1706

Amino Acids

197.96

Weight (kDa)

8.84

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 51 - 180 2.4e-10 Vta1 like
FKS1_dom1 PF14288 336 - 447 1.5e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 857 - 1040 5e-70 Callose synthase 1 domain
Glucan_synthase PF02364 1047 - 1137 1.2e-29 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1140 - 1279 7.8e-24 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1283 - 1518 3.3e-50 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1205, 1842, 2474, 2954
AasI GACNNNNNNGTC 1 cut(s) 858
AatII GACGTC 2 cut(s) 1584, 3883
Acc36I ACCTGC 2 cut(s) 502, 3342
Acc65I GGTACC 1 cut(s) 490
AccB1I GGYRCC 1 cut(s) 490
AccB7I CCANNNNNTGG 3 cut(s) 1099, 3151, 4863
AccI GTMKAC 4 cut(s) 716, 2784, 2794, 4708
AccII CGCG 1 cut(s) 34
AciI CCGC 9 cut(s) 15, 34, 38, 146, 217, 1825, 2093, 2326, 3376
AclWI GGATC 8 cut(s) 245, 258, 1561, 2048, 2526, 3910, 4165, 4478
AcoI YGGCCR 4 cut(s) 35, 438, 1337, 2977
AcuI CTGAAG 7 cut(s) 552, 1570, 1786, 2272, 2334, 3702, 4772
AcyI GRCGYC 2 cut(s) 1581, 3880
AdeI CACNNNGTG 2 cut(s) 4246, 4863
AfaI GTAC 9 cut(s) 465, 492, 897, 1173, 1229, 2681, 3401, 3488, 4229
AfeI AGCGCT 1 cut(s) 639
AfiI CCNNNNNNNGG 7 cut(s) 33, 101, 1099, 2299, 3151, 4012, 4863
AflIII ACRYGT 1 cut(s) 3745
AjiI CACGTC 1 cut(s) 269
AjnI CCWGG 7 cut(s) 440, 1218, 2214, 3369, 3519, 3918, 4361
AjuI GAANNNNNNNTTGG 2 cut(s) 2396, 2428
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
Alw21I GWGCWC 4 cut(s) 11, 480, 649, 4680
Alw26I GTCTC 3 cut(s) 2699, 3684, 5020
AlwI GGATC 8 cut(s) 245, 258, 1561, 2048, 2526, 3910, 4165, 4478
AlwNI CAGNNNCTG 1 cut(s) 1727
Ama87I CYCGRG 1 cut(s) 3687
Aor51HI AGCGCT 1 cut(s) 639
ArsI GACNNNNNNTTYG 4 cut(s) 258, 280, 290, 312
AseI ATTAAT 1 cut(s) 2817
Asp700I GAANNNNTTC 2 cut(s) 1799, 3270
Asp718I GGTACC 1 cut(s) 490
AspA2I CCTAGG 1 cut(s) 197
AspLEI GCGC 6 cut(s) 34, 241, 640, 1941, 3168, 4759
AspS9I GGNCC 5 cut(s) 18, 1401, 2328, 3373, 4003
AsuC2I CCSGG 2 cut(s) 167, 5089
AsuHPI GGTGA 5 cut(s) 1055, 3674, 3839, 4070, 4467
AsuII TTCGAA 1 cut(s) 2431
AsuNHI GCTAGC 3 cut(s) 2062, 2585, 2881
AvaI CYCGRG 1 cut(s) 3687
AvaII GGWCC 5 cut(s) 18, 1401, 2328, 3373, 4003
AvrII CCTAGG 1 cut(s) 197
BaeGI GKGCMC 3 cut(s) 3144, 3610, 4870
BaeI ACNNNNGTAYC 2 cut(s) 474, 507
BalI TGGCCA 3 cut(s) 440, 1339, 2979
BamHI GGATCC 1 cut(s) 250
BanI GGYRCC 1 cut(s) 490
BanII GRGCYC 2 cut(s) 11, 1969
BbsI GAAGAC 6 cut(s) 1576, 1921, 2002, 2499, 3779, 4969
Bbv12I GWGCWC 4 cut(s) 11, 480, 649, 4680
BceAI ACGGC 1 cut(s) 118
BciT130I CCWGG 7 cut(s) 442, 1220, 2216, 3371, 3521, 3920, 4363
BciVI GTATCC 3 cut(s) 1374, 3527, 3744
BclI TGATCA 2 cut(s) 2499, 2970
BcnI CCSGG 2 cut(s) 167, 5089
BcoDI GTCTC 3 cut(s) 2699, 3684, 5020
BfmI CTRYAG 8 cut(s) 650, 726, 1474, 1782, 3335, 3490, 4079, 4416
BfoI RGCGCY 2 cut(s) 641, 4760
BfuAI ACCTGC 2 cut(s) 502, 3342
BfuI GTATCC 3 cut(s) 1374, 3527, 3744
BlnI CCTAGG 1 cut(s) 197
BlpI GCTNAGC 1 cut(s) 1968
Bme1390I CCNGG 9 cut(s) 167, 442, 1220, 2216, 3371, 3521, 3920, 4363, 5089
Bme18I GGWCC 5 cut(s) 18, 1401, 2328, 3373, 4003
BmeT110I CYCGRG 1 cut(s) 3687
BmgBI CACGTC 1 cut(s) 269
BmgT120I GGNCC 5 cut(s) 18, 1401, 2328, 3373, 4003
BmiI GGNNCC 7 cut(s) 19, 252, 492, 974, 1869, 2284, 5006
BmrFI CCNGG 9 cut(s) 167, 442, 1220, 2216, 3371, 3521, 3920, 4363, 5089
BmrI ACTGGG 1 cut(s) 3998
BmsI GCATC 9 cut(s) 19, 100, 301, 1336, 1564, 1611, 2221, 2383, 3145
BmtI GCTAGC 3 cut(s) 2066, 2589, 2885
BmuI ACTGGG 1 cut(s) 3998
BpiI GAAGAC 6 cut(s) 1576, 1921, 2002, 2499, 3779, 4969
BpmI CTGGAG 1 cut(s) 4052
Bpu1102I GCTNAGC 1 cut(s) 1968
Bpu14I TTCGAA 1 cut(s) 2431
BpuEI CTTGAG 3 cut(s) 464, 3701, 4508
BpuMI CCSGG 2 cut(s) 167, 5089
BsaAI YACGTR 2 cut(s) 1175, 3746
BsaBI GATNNNNATC 2 cut(s) 2517, 4469
BsaHI GRCGYC 2 cut(s) 1581, 3880
BsaJI CCNNGG 9 cut(s) 21, 197, 223, 246, 3297, 3945, 4011, 4053, 4540
BsaWI WCCGGW 1 cut(s) 427
BsaXI ACNNNNNCTCC 6 cut(s) 3023, 3053, 3218, 3248, 4959, 4989
Bsc4I CCNNNNNNNGG 7 cut(s) 33, 101, 1099, 2299, 3151, 4012, 4863
Bse118I RCCGGY 1 cut(s) 4984
Bse1I ACTGG 8 cut(s) 1388, 1420, 1940, 2563, 3993, 4180, 4409, 4644
Bse3DI GCAATG 4 cut(s) 1017, 1138, 2190, 2439
Bse8I GATNNNNATC 2 cut(s) 2517, 4469
BseBI CCWGG 7 cut(s) 442, 1220, 2216, 3371, 3521, 3920, 4363
BseDI CCNNGG 9 cut(s) 21, 197, 223, 246, 3297, 3945, 4011, 4053, 4540
BseJI GATNNNNATC 2 cut(s) 2517, 4469
BseLI CCNNNNNNNGG 7 cut(s) 33, 101, 1099, 2299, 3151, 4012, 4863
BseMI GCAATG 4 cut(s) 1017, 1138, 2190, 2439
BseMII CTCAG 7 cut(s) 615, 855, 1672, 1982, 2468, 2725, 5031
BseNI ACTGG 8 cut(s) 1388, 1420, 1940, 2563, 3993, 4180, 4409, 4644
BseRI GAGGAG 1 cut(s) 3599
BseSI GKGCMC 3 cut(s) 3144, 3610, 4870
BseX3I CGGCCG 1 cut(s) 35
BseYI CCCAGC 2 cut(s) 3928, 4939
BsgI GTGCAG 1 cut(s) 4912
Bsh1236I CGCG 1 cut(s) 34
Bsh1285I CGRYCG 1 cut(s) 38
BshNI GGYRCC 1 cut(s) 490
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 4 cut(s) 11, 480, 649, 4680
BsiHKCI CYCGRG 1 cut(s) 3687
BsiSI CCGG 4 cut(s) 166, 428, 4985, 5088
BslFI GGGAC 4 cut(s) 31, 329, 3465, 4309
BslI CCNNNNNNNGG 7 cut(s) 33, 101, 1099, 2299, 3151, 4012, 4863
BsmAI GTCTC 3 cut(s) 2699, 3684, 5020
BsmFI GGGAC 4 cut(s) 31, 329, 3465, 4309
BsmI GAATGC 8 cut(s) 687, 1079, 1369, 1672, 2001, 2170, 2702, 5051
BsoBI CYCGRG 1 cut(s) 3687
Bsp119I TTCGAA 1 cut(s) 2431
Bsp1286I GDGCHC 8 cut(s) 11, 480, 649, 1969, 3144, 3610, 4680, 4870
Bsp1407I TGTACA 2 cut(s) 895, 1227
Bsp1720I GCTNAGC 1 cut(s) 1968
Bsp19I CCATGG 3 cut(s) 223, 246, 4011
BspACI CCGC 9 cut(s) 15, 34, 38, 146, 217, 1825, 2093, 2326, 3376
BspCNI CTCAG 7 cut(s) 616, 856, 1671, 1981, 2467, 2724, 5030
BspFNI CGCG 1 cut(s) 34
BspHI TCATGA 2 cut(s) 3195, 4785
BspLI GGNNCC 7 cut(s) 19, 252, 492, 974, 1869, 2284, 5006
BspMAI CTGCAG 2 cut(s) 3339, 4083
BspMI ACCTGC 2 cut(s) 502, 3342
BspOI GCTAGC 3 cut(s) 2066, 2589, 2885
BspPI GGATC 8 cut(s) 245, 258, 1561, 2048, 2526, 3910, 4165, 4478
BspQI GCTCTTC 3 cut(s) 1670, 4939, 5045
BspT104I TTCGAA 1 cut(s) 2431
BspT107I GGYRCC 1 cut(s) 490
BsrDI GCAATG 4 cut(s) 1017, 1138, 2190, 2439
BsrFI RCCGGY 1 cut(s) 4984
BsrGI TGTACA 2 cut(s) 895, 1227
BsrI ACTGG 8 cut(s) 1388, 1420, 1940, 2563, 3993, 4180, 4409, 4644
BssAI RCCGGY 1 cut(s) 4984
BssECI CCNNGG 9 cut(s) 21, 197, 223, 246, 3297, 3945, 4011, 4053, 4540
BssNAI GTATAC 1 cut(s) 2795
BssNI GRCGYC 2 cut(s) 1581, 3880
BssT1I CCWWGG 8 cut(s) 21, 197, 223, 246, 3297, 3945, 4011, 4053
Bst1107I GTATAC 1 cut(s) 2795
Bst2UI CCWGG 7 cut(s) 442, 1220, 2216, 3371, 3521, 3920, 4363
Bst6I CTCTTC 6 cut(s) 426, 1670, 1984, 4433, 4939, 5045
BstACI GRCGYC 2 cut(s) 1581, 3880
BstAPI GCANNNNNTGC 2 cut(s) 298, 4208
BstAUI TGTACA 2 cut(s) 895, 1227
BstBAI YACGTR 2 cut(s) 1175, 3746
BstBI TTCGAA 1 cut(s) 2431
BstDEI CTNAG 8 cut(s) 624, 864, 1658, 1968, 2454, 2711, 2846, 5017
BstDSI CCRYGG 4 cut(s) 223, 246, 4011, 4540
BstFNI CGCG 1 cut(s) 34
BstH2I RGCGCY 2 cut(s) 641, 4760
BstHHI GCGC 6 cut(s) 34, 241, 640, 1941, 3168, 4759
BstMAI GTCTC 3 cut(s) 2699, 3684, 5020
BstMCI CGRYCG 1 cut(s) 38
BstNI CCWGG 7 cut(s) 442, 1220, 2216, 3371, 3521, 3920, 4363
BstNSI RCATGY 3 cut(s) 3205, 3232, 4572
BstSCI CCNGG 9 cut(s) 165, 440, 1218, 2214, 3369, 3519, 3918, 4361, 5087
BstSFI CTRYAG 8 cut(s) 650, 726, 1474, 1782, 3335, 3490, 4079, 4416
BstSLI GKGCMC 3 cut(s) 3144, 3610, 4870
BstUI CGCG 1 cut(s) 34
BstV2I GAAGAC 6 cut(s) 1576, 1921, 2002, 2499, 3779, 4969
BstX2I RGATCY 6 cut(s) 250, 1553, 2518, 3915, 4157, 4470
BstXI CCANNNNNNTGG 1 cut(s) 4018
BstYI RGATCY 6 cut(s) 250, 1553, 2518, 3915, 4157, 4470
BstZ17I GTATAC 1 cut(s) 2795
BstZI CGGCCG 1 cut(s) 35
BsuI GTATCC 3 cut(s) 1374, 3527, 3744
BtgI CCRYGG 4 cut(s) 223, 246, 4011, 4540
BtrI CACGTC 1 cut(s) 269
BtsI GCAGTG 3 cut(s) 2015, 2694, 4047
BveI ACCTGC 2 cut(s) 502, 3342
CaiI CAGNNNCTG 1 cut(s) 1727
CciI TCATGA 2 cut(s) 3195, 4785
CciNI GCGGCCGC 1 cut(s) 35
CfoI GCGC 6 cut(s) 34, 241, 640, 1941, 3168, 4759
Cfr10I RCCGGY 1 cut(s) 4984
Cfr13I GGNCC 5 cut(s) 18, 1401, 2328, 3373, 4003
CseI GACGC 2 cut(s) 298, 2903
CsiI ACCWGGT 1 cut(s) 1218
Csp6I GTAC 9 cut(s) 464, 491, 896, 1172, 1228, 2680, 3400, 3487, 4228
CviQI GTAC 9 cut(s) 464, 491, 896, 1172, 1228, 2680, 3400, 3487, 4228
DdeI CTNAG 8 cut(s) 624, 864, 1658, 1968, 2454, 2711, 2846, 5017
DraI TTTAAA 2 cut(s) 691, 1125
DraIII CACNNNGTG 2 cut(s) 4246, 4863
DrdI GACNNNNNNGTC 1 cut(s) 858
DseDI GACNNNNNNGTC 1 cut(s) 858
EaeI YGGCCR 4 cut(s) 35, 438, 1337, 2977
EagI CGGCCG 1 cut(s) 35
Eam1104I CTCTTC 6 cut(s) 426, 1670, 1984, 4433, 4939, 5045
EarI CTCTTC 6 cut(s) 426, 1670, 1984, 4433, 4939, 5045
EciI GGCGGA 1 cut(s) 1840
Ecl136II GAGCTC 1 cut(s) 9
EclXI CGGCCG 1 cut(s) 35
Eco130I CCWWGG 8 cut(s) 21, 197, 223, 246, 3297, 3945, 4011, 4053
Eco147I AGGCCT 1 cut(s) 5064
Eco24I GRGCYC 2 cut(s) 11, 1969
Eco32I GATATC 1 cut(s) 2941
Eco47I GGWCC 5 cut(s) 18, 1401, 2328, 3373, 4003
Eco47III AGCGCT 1 cut(s) 639
Eco52I CGGCCG 1 cut(s) 35
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 7 cut(s) 552, 1570, 1786, 2272, 2334, 3702, 4772
Eco88I CYCGRG 1 cut(s) 3687
EcoICRI GAGCTC 1 cut(s) 9
EcoRI GAATTC 2 cut(s) 3673, 4746
EcoRII CCWGG 7 cut(s) 440, 1218, 2214, 3369, 3519, 3918, 4361
EcoRV GATATC 1 cut(s) 2941
EcoT14I CCWWGG 8 cut(s) 21, 197, 223, 246, 3297, 3945, 4011, 4053
EcoT22I ATGCAT 3 cut(s) 687, 1672, 2001
EcoT38I GRGCYC 2 cut(s) 11, 1969
ErhI CCWWGG 8 cut(s) 21, 197, 223, 246, 3297, 3945, 4011, 4053
FalI AAGNNNNNCTT 4 cut(s) 2396, 2428, 3258, 3290
FaqI GGGAC 4 cut(s) 31, 329, 3465, 4309
FauI CCCGC 1 cut(s) 8
FauNDI CATATG 2 cut(s) 1037, 1615
FbaI TGATCA 2 cut(s) 2499, 2970
FblI GTMKAC 4 cut(s) 716, 2784, 2794, 4708
FriOI GRGCYC 2 cut(s) 11, 1969
GlaI GCGC 6 cut(s) 33, 240, 639, 1940, 3167, 4758
GsaI CCCAGC 2 cut(s) 3932, 4943
GsuI CTGGAG 1 cut(s) 4052
HaeII RGCGCY 2 cut(s) 641, 4760
HapII CCGG 4 cut(s) 166, 428, 4985, 5088
HgaI GACGC 2 cut(s) 298, 2903
HhaI GCGC 6 cut(s) 34, 241, 640, 1941, 3168, 4759
Hin1I GRCGYC 2 cut(s) 1581, 3880
Hin6I GCGC 6 cut(s) 32, 239, 638, 1939, 3166, 4757
HinP1I GCGC 6 cut(s) 32, 239, 638, 1939, 3166, 4757
HincII GTYRAC 2 cut(s) 717, 3729
HindII GTYRAC 2 cut(s) 717, 3729
HindIII AAGCTT 1 cut(s) 1047
HpaI GTTAAC 1 cut(s) 3729
HpaII CCGG 4 cut(s) 166, 428, 4985, 5088
HphI GGTGA 5 cut(s) 1055, 3674, 3839, 4070, 4467
Hpy99I CGWCG 3 cut(s) 100, 110, 139
HpyCH4IV ACGT 8 cut(s) 268, 846, 1174, 1581, 3731, 3745, 3880, 4334
HpyF3I CTNAG 8 cut(s) 624, 864, 1658, 1968, 2454, 2711, 2846, 5017
HpySE526I ACGT 8 cut(s) 268, 846, 1174, 1581, 3731, 3745, 3880, 4334
Hsp92I GRCGYC 2 cut(s) 1581, 3880
HspAI GCGC 6 cut(s) 32, 239, 638, 1939, 3166, 4757
KpnI GGTACC 1 cut(s) 494
Ksp22I TGATCA 2 cut(s) 2499, 2970
KspAI GTTAAC 1 cut(s) 3729
LguI GCTCTTC 3 cut(s) 1670, 4939, 5045
LweI GCATC 9 cut(s) 19, 100, 301, 1336, 1564, 1611, 2221, 2383, 3145
MabI ACCWGGT 1 cut(s) 1218
MaeII ACGT 8 cut(s) 268, 846, 1174, 1581, 3731, 3745, 3880, 4334
MaeIII GTNAC 7 cut(s) 1687, 1945, 3224, 3701, 3827, 3844, 4238
MfeI CAATTG 1 cut(s) 1001
MflI RGATCY 6 cut(s) 250, 1553, 2518, 3915, 4157, 4470
MhlI GDGCHC 8 cut(s) 11, 480, 649, 1969, 3144, 3610, 4680, 4870
MlsI TGGCCA 3 cut(s) 440, 1339, 2979
MluNI TGGCCA 3 cut(s) 440, 1339, 2979
MlyI GAGTC 7 cut(s) 300, 1552, 1822, 2197, 2348, 3244, 4296
Mox20I TGGCCA 3 cut(s) 440, 1339, 2979
Mph1103I ATGCAT 3 cut(s) 687, 1672, 2001
MroXI GAANNNNTTC 2 cut(s) 1799, 3270
MscI TGGCCA 3 cut(s) 440, 1339, 2979
MslI CAYNNNNRTG 9 cut(s) 1374, 1607, 2004, 2239, 2415, 3137, 3200, 4016, 4070
Msp20I TGGCCA 3 cut(s) 440, 1339, 2979
MspA1I CMGCKG 3 cut(s) 15, 4078, 4084
MspI CCGG 4 cut(s) 166, 428, 4985, 5088
MspR9I CCNGG 9 cut(s) 167, 442, 1220, 2216, 3371, 3521, 3920, 4363, 5089
MunI CAATTG 1 cut(s) 1001
Mva1269I GAATGC 8 cut(s) 687, 1079, 1369, 1672, 2001, 2170, 2702, 5051
MvaI CCWGG 7 cut(s) 442, 1220, 2216, 3371, 3521, 3920, 4363
MvnI CGCG 1 cut(s) 34
NciI CCSGG 2 cut(s) 167, 5089
NcoI CCATGG 3 cut(s) 223, 246, 4011
NdeI CATATG 2 cut(s) 1037, 1615
NheI GCTAGC 3 cut(s) 2062, 2585, 2881
NlaIV GGNNCC 7 cut(s) 19, 252, 492, 974, 1869, 2284, 5006
NmeAIII GCCGAG 1 cut(s) 2663
NmuCI GTSAC 5 cut(s) 1687, 1945, 3224, 3827, 4238
NotI GCGGCCGC 1 cut(s) 35
NsiI ATGCAT 3 cut(s) 687, 1672, 2001
NspI RCATGY 3 cut(s) 3205, 3232, 4572
NspV TTCGAA 1 cut(s) 2431
PagI TCATGA 2 cut(s) 3195, 4785
PceI AGGCCT 1 cut(s) 5064
PciSI GCTCTTC 3 cut(s) 1670, 4939, 5045
PcsI WCGNNNNNNNCGW 2 cut(s) 274, 315
PctI GAATGC 8 cut(s) 687, 1079, 1369, 1672, 2001, 2170, 2702, 5051
PdmI GAANNNNTTC 2 cut(s) 1799, 3270
PflMI CCANNNNNTGG 3 cut(s) 1099, 3151, 4863
PfoI TCCNGGA 3 cut(s) 3369, 3519, 3918
PleI GAGTC 7 cut(s) 300, 1551, 1821, 2197, 2347, 3244, 4295
PpsI GAGTC 7 cut(s) 300, 1551, 1821, 2197, 2347, 3244, 4295
Ppu21I YACGTR 2 cut(s) 1175, 3746
PshBI ATTAAT 1 cut(s) 2817
PsiI TTATAA 4 cut(s) 1205, 1842, 2474, 2954
Psp124BI GAGCTC 1 cut(s) 11
Psp6I CCWGG 7 cut(s) 440, 1218, 2214, 3369, 3519, 3918, 4361
PspFI CCCAGC 2 cut(s) 3928, 4939
PspGI CCWGG 7 cut(s) 440, 1218, 2214, 3369, 3519, 3918, 4361
PspN4I GGNNCC 7 cut(s) 19, 252, 492, 974, 1869, 2284, 5006
PspPI GGNCC 5 cut(s) 18, 1401, 2328, 3373, 4003
PstI CTGCAG 2 cut(s) 3339, 4083
PstNI CAGNNNCTG 1 cut(s) 1727
PsuI RGATCY 6 cut(s) 250, 1553, 2518, 3915, 4157, 4470
PvuII CAGCTG 2 cut(s) 4078, 4084
RsaI GTAC 9 cut(s) 465, 492, 897, 1173, 1229, 2681, 3401, 3488, 4229
RsaNI GTAC 9 cut(s) 464, 491, 896, 1172, 1228, 2680, 3400, 3487, 4228
RseI CAYNNNNRTG 9 cut(s) 1374, 1607, 2004, 2239, 2415, 3137, 3200, 4016, 4070
SacI GAGCTC 1 cut(s) 11
SalI GTCGAC 1 cut(s) 715
SapI GCTCTTC 3 cut(s) 1670, 4939, 5045
Sau96I GGNCC 5 cut(s) 18, 1401, 2328, 3373, 4003
SchI GAGTC 7 cut(s) 300, 1552, 1822, 2197, 2348, 3244, 4296
ScrFI CCNGG 9 cut(s) 167, 442, 1220, 2216, 3371, 3521, 3920, 4363, 5089
SduI GDGCHC 8 cut(s) 11, 480, 649, 1969, 3144, 3610, 4680, 4870
SexAI ACCWGGT 1 cut(s) 1218
SfaNI GCATC 9 cut(s) 19, 100, 301, 1336, 1564, 1611, 2221, 2383, 3145
SfcI CTRYAG 8 cut(s) 650, 726, 1474, 1782, 3335, 3490, 4079, 4416
SfuI TTCGAA 1 cut(s) 2431
SinI GGWCC 5 cut(s) 18, 1401, 2328, 3373, 4003
SmiMI CAYNNNNRTG 9 cut(s) 1374, 1607, 2004, 2239, 2415, 3137, 3200, 4016, 4070
SmlI CTYRAG 3 cut(s) 479, 3716, 4523
SmoI CTYRAG 3 cut(s) 479, 3716, 4523
SseBI AGGCCT 1 cut(s) 5064
SsiI CCGC 9 cut(s) 15, 34, 38, 146, 217, 1825, 2093, 2326, 3376
SspI AATATT 5 cut(s) 1569, 1698, 2179, 2253, 3712
SstI GAGCTC 1 cut(s) 11
StuI AGGCCT 1 cut(s) 5064
StyD4I CCNGG 9 cut(s) 165, 440, 1218, 2214, 3369, 3519, 3918, 4361, 5087
StyI CCWWGG 8 cut(s) 21, 197, 223, 246, 3297, 3945, 4011, 4053
TaiI ACGT 8 cut(s) 271, 849, 1177, 1584, 3734, 3748, 3883, 4337
TatI WGTACW 7 cut(s) 463, 895, 1227, 2679, 3399, 3486, 4227
TauI GCSGC 5 cut(s) 37, 40, 149, 219, 2095
TseFI GTSAC 5 cut(s) 1687, 1945, 3224, 3827, 4238
Tsp45I GTSAC 5 cut(s) 1687, 1945, 3224, 3827, 4238
TspGWI ACGGA 9 cut(s) 113, 632, 2295, 2523, 2813, 4149, 4380, 4445, 4529
Van91I CCANNNNNTGG 3 cut(s) 1099, 3151, 4863
VpaK11BI GGWCC 5 cut(s) 18, 1401, 2328, 3373, 4003
VspI ATTAAT 1 cut(s) 2817
XbaI TCTAGA 2 cut(s) 3464, 3502
XceI RCATGY 3 cut(s) 3205, 3232, 4572
XcmI CCANNNNNNNNNTGG 2 cut(s) 4348, 4485
XmaJI CCTAGG 1 cut(s) 197
XmiI GTMKAC 4 cut(s) 716, 2784, 2794, 4708
XmnI GAANNNNTTC 2 cut(s) 1799, 3270
ZraI GACGTC 2 cut(s) 1582, 3881
Zsp2I ATGCAT 3 cut(s) 687, 1672, 2001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.