Rorug02G0428500

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009173
Physical Location & Seq
Forward (+)
54914731 .. 54919959
5229 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0428500.1

Sequence Viewer

Length: 1197 bp
ATGGCATCAGCATTCTGTAATCTCAACCCGTGCGGCTCTGGTCTCACAAAACTAAATGACTACAAGAAGCACACAGTCCTCAAAAGGCCTTACATAGACTGCAAAAGATTGTCTCTTTCGCCTTGCAATGCCGAAAAGGTTTTCCCCAAGACTCTTAGAAGACAAGTAGAGGTCATGCATTGCACACAGGCTTTTGTCAATAGTAATGGTGCGGATTTGGGTACTGTCCATGGGGAGGATAAAGAAGAGAGTATTTCTAAGGCAACCTTAATATGGAGGGCAATAAAGTTGCCAATATACTCTGTTGCATTCATCCCTCTCACTGTAGGTAGTGCAGCTGCTTATTTAGAGACGGGCATATTTTCATCATGGCGTTATTCTATTCTTTTGATTTCTTCTGTTTTTATCATCACATGGCTCAACTTAAGCAATGATGTTTATGATTTCGATACAGGAGCAGATAAAAACAAGGTAGAATCGGTTGTAAATCTGGTTGGCAGCCGTACAGGCACAATTGTTGCTGCCTACTCGCTACTTGTTCTTGGCTTCATGGGGCTTACTTGGGTATCTTTTGAGGCAGGAAATATGCGGGCAATATTATTACTCGCTTGTGCAATAATTTTTGGTTATATATATCAGTGCCCACCATTTCGGTTAAGTTACCAAGGATTGGGGGAGCCATTGTGCTTTGCAGCATTTGGTCCATTTGCCACTACAGCTTTTTATTTACTGCAGGGCAGCGGAAGTAAAATGATGCATCTTCCTTTCACTGGTCCAATTCTTTCTGCATCACTTCTTGTTGGCATTACGACAACCTTAATTCTGTTCTGTAGTCACTTTCATCAGATTGAAGAAGACAAGGCGGTAGAAAAAATTTCCCCTCTGGTTAGGCTTGGCACCAAAAGAGGATCATGGGTAGTGAGATTGGCAATTATAGGACTGTATGTCCTAGCTTTTTCTTTTGGTCTAAGCAGGGCTCTTCCCATTACTTGTATATTTTTCTGTGCCTTGACCTTACCAGTGGCAAGACTCGTGGTTAGCTATGTTCAGGAAAACCATAATGATAAACATAAAATATTCATGGCGAAGTATTACTGTGTGAGATTGCATTCTTTATTTGGAGTTGCACTCGCTGCTGGACTAGTGGTAGCGAGAACGGCCCCTAAAATTAGTATTCCAAGGTTGGCATTTTCATAG

Protein Analysis

398

Amino Acids

43.74

Weight (kDa)

9.25

Isoelectric Point (pI)

31.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UbiA PF01040 104 - 362 1.6e-25 UbiA prenyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 896
AccB7I CCANNNNNTGG 1 cut(s) 670
AciI CCGC 5 cut(s) 33, 212, 589, 741, 863
AclWI GGATC 1 cut(s) 916
AcsI RAATTY 1 cut(s) 873
AfaI GTAC 2 cut(s) 223, 505
AfiI CCNNNNNNNGG 4 cut(s) 235, 273, 670, 770
AflII CTTAAG 1 cut(s) 424
AgsI TTSAA 1 cut(s) 851
AhdI GACNNNNNGTC 1 cut(s) 944
AhlI ACTAGT 1 cut(s) 1141
AluBI AGCT 4 cut(s) 338, 719, 953, 1041
AluI AGCT 4 cut(s) 338, 719, 953, 1041
Alw26I GTCTC 3 cut(s) 47, 117, 344
AlwI GGATC 1 cut(s) 916
AoxI GGCC 2 cut(s) 86, 1158
ApeKI GCWGC 7 cut(s) 335, 338, 498, 521, 692, 738, 1133
ApoI RAATTY 1 cut(s) 873
AspS9I GGNCC 3 cut(s) 701, 773, 1159
AvaII GGWCC 2 cut(s) 701, 773
BaeGI GKGCMC 1 cut(s) 644
BaeI ACNNNNGTAYC 2 cut(s) 549, 582
BanI GGYRCC 1 cut(s) 896
BanII GRGCYC 1 cut(s) 979
BauI CACGAG 1 cut(s) 1031
BbsI GAAGAC 2 cut(s) 166, 861
BbvI GCAGC 7 cut(s) 325, 347, 508, 510, 704, 750, 1120
BceAI ACGGC 2 cut(s) 486, 1173
BcoDI GTCTC 3 cut(s) 47, 117, 344
BcuI ACTAGT 1 cut(s) 1141
BfaI CTAG 2 cut(s) 950, 1142
BfmI CTRYAG 4 cut(s) 324, 714, 731, 829
BfrI CTTAAG 1 cut(s) 424
BglI GCCNNNNNGGC 1 cut(s) 507
BisI GCNGC 8 cut(s) 34, 336, 339, 499, 522, 693, 739, 1134
BlsI GCNGC 8 cut(s) 35, 337, 340, 500, 523, 694, 740, 1135
Bme18I GGWCC 2 cut(s) 701, 773
BmeRI GACNNNNNGTC 1 cut(s) 944
BmgT120I GGNCC 3 cut(s) 701, 773, 1159
BmiI GGNNCC 3 cut(s) 678, 898, 1161
BmsI GCATC 4 cut(s) 14, 744, 766, 797
BpiI GAAGAC 2 cut(s) 166, 861
BplI GAGNNNNNCTC 2 cut(s) 1113, 1145
BsaI GGTCTC 1 cut(s) 47
BsaJI CCNNGG 3 cut(s) 229, 664, 1178
BsaXI ACNNNNNCTCC 2 cut(s) 668, 698
Bsc4I CCNNNNNNNGG 4 cut(s) 235, 273, 670, 770
Bse1I ACTGG 2 cut(s) 775, 1019
Bse3DI GCAATG 3 cut(s) 133, 178, 436
BseDI CCNNGG 3 cut(s) 229, 664, 1178
BseGI GGATG 1 cut(s) 312
BseLI CCNNNNNNNGG 4 cut(s) 235, 273, 670, 770
BseMI GCAATG 3 cut(s) 133, 178, 436
BseNI ACTGG 2 cut(s) 775, 1019
BseSI GKGCMC 1 cut(s) 644
BseXI GCAGC 7 cut(s) 325, 347, 508, 510, 704, 750, 1120
BsgI GTGCAG 1 cut(s) 354
BshFI GGCC 2 cut(s) 88, 1160
BshNI GGYRCC 1 cut(s) 896
BslI CCNNNNNNNGG 4 cut(s) 235, 273, 670, 770
BsmAI GTCTC 3 cut(s) 47, 117, 344
BsmBI CGTCTC 1 cut(s) 344
BsmI GAATGC 3 cut(s) 11, 308, 1108
BsnI GGCC 2 cut(s) 88, 1160
Bso31I GGTCTC 1 cut(s) 47
Bsp1286I GDGCHC 2 cut(s) 644, 979
Bsp143I GATC 1 cut(s) 908
Bsp19I CCATGG 1 cut(s) 229
BspACI CCGC 5 cut(s) 33, 212, 589, 741, 863
BspANI GGCC 2 cut(s) 88, 1160
BspLI GGNNCC 3 cut(s) 678, 898, 1161
BspMAI CTGCAG 1 cut(s) 735
BspPI GGATC 1 cut(s) 916
BspQI GCTCTTC 1 cut(s) 984
BspT107I GGYRCC 1 cut(s) 896
BspTI CTTAAG 1 cut(s) 424
BspTNI GGTCTC 1 cut(s) 47
BsrDI GCAATG 3 cut(s) 133, 178, 436
BsrI ACTGG 2 cut(s) 775, 1019
BssECI CCNNGG 3 cut(s) 229, 664, 1178
BssMI GATC 1 cut(s) 908
BssSI CACGAG 1 cut(s) 1031
BssT1I CCWWGG 3 cut(s) 229, 664, 1178
Bst2BI CACGAG 1 cut(s) 1031
Bst4CI ACNGT 5 cut(s) 76, 226, 325, 942, 1097
Bst6I CTCTTC 2 cut(s) 240, 984
BstAFI CTTAAG 1 cut(s) 424
BstAPI GCANNNNNTGC 1 cut(s) 1133
BstC8I GCNNGC 1 cut(s) 591
BstDEI CTNAG 3 cut(s) 155, 258, 968
BstDSI CCRYGG 1 cut(s) 229
BstF5I GGATG 1 cut(s) 312
BstKTI GATC 1 cut(s) 911
BstMAI GTCTC 3 cut(s) 47, 117, 344
BstMBI GATC 1 cut(s) 908
BstMWI GCNNNNNNNGC 4 cut(s) 507, 716, 1133, 1157
BstSFI CTRYAG 4 cut(s) 324, 714, 731, 829
BstSLI GKGCMC 1 cut(s) 644
BstV1I GCAGC 7 cut(s) 325, 347, 508, 510, 704, 750, 1120
BstV2I GAAGAC 2 cut(s) 166, 861
BsuRI GGCC 2 cut(s) 88, 1160
BtgI CCRYGG 1 cut(s) 229
BtsCI GGATG 1 cut(s) 312
BtsIMutI CAGTG 4 cut(s) 321, 644, 768, 1026
Cac8I GCNNGC 1 cut(s) 591
Cfr13I GGNCC 3 cut(s) 701, 773, 1159
Csp6I GTAC 2 cut(s) 222, 504
CspCI CAANNNNNGTGG 2 cut(s) 1014, 1049
CviAII CATG 7 cut(s) 175, 230, 369, 414, 550, 912, 1081
CviQI GTAC 2 cut(s) 222, 504
DdeI CTNAG 3 cut(s) 155, 258, 968
DpnI GATC 1 cut(s) 910
DpnII GATC 1 cut(s) 908
DriI GACNNNNNGTC 1 cut(s) 944
Eam1104I CTCTTC 2 cut(s) 240, 984
Eam1105I GACNNNNNGTC 1 cut(s) 944
EarI CTCTTC 2 cut(s) 240, 984
Eco130I CCWWGG 3 cut(s) 229, 664, 1178
Eco147I AGGCCT 1 cut(s) 88
Eco24I GRGCYC 1 cut(s) 979
Eco31I GGTCTC 1 cut(s) 47
Eco47I GGWCC 2 cut(s) 701, 773
EcoT14I CCWWGG 3 cut(s) 229, 664, 1178
EcoT22I ATGCAT 2 cut(s) 180, 759
EcoT38I GRGCYC 1 cut(s) 979
ErhI CCWWGG 3 cut(s) 229, 664, 1178
Esp3I CGTCTC 1 cut(s) 344
FaeI CATG 7 cut(s) 178, 233, 372, 417, 553, 915, 1084
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FatI CATG 7 cut(s) 174, 229, 368, 413, 549, 911, 1080
FauI CCCGC 1 cut(s) 582
Fnu4HI GCNGC 8 cut(s) 34, 336, 339, 499, 522, 693, 739, 1134
FokI GGATG 1 cut(s) 299
FriOI GRGCYC 1 cut(s) 979
Fsp4HI GCNGC 8 cut(s) 34, 336, 339, 499, 522, 693, 739, 1134
FspBI CTAG 2 cut(s) 950, 1142
GluI GCNGC 8 cut(s) 34, 336, 339, 499, 522, 693, 739, 1134
HaeIII GGCC 2 cut(s) 88, 1160
Hin1II CATG 7 cut(s) 178, 233, 372, 417, 553, 915, 1084
HinfI GANTC 3 cut(s) 151, 476, 1029
Hpy188I TCNGA 1 cut(s) 846
Hpy188III TCNNGA 1 cut(s) 1049
HpyCH4III ACNGT 5 cut(s) 76, 226, 325, 942, 1097
HpyF10VI GCNNNNNNNGC 4 cut(s) 507, 716, 1133, 1157
HpyF3I CTNAG 3 cut(s) 155, 258, 968
Hsp92II CATG 7 cut(s) 178, 233, 372, 417, 553, 915, 1084
Kzo9I GATC 1 cut(s) 908
LguI GCTCTTC 1 cut(s) 984
LmnI GCTCC 2 cut(s) 455, 676
Lsp1109I GCAGC 7 cut(s) 325, 347, 508, 510, 704, 750, 1120
LweI GCATC 4 cut(s) 14, 744, 766, 797
MaeI CTAG 2 cut(s) 950, 1142
MaeIII GTNAC 2 cut(s) 659, 833
MalI GATC 1 cut(s) 910
MboI GATC 1 cut(s) 908
MboII GAAGA 7 cut(s) 171, 257, 387, 752, 863, 866, 971
MfeI CAATTG 1 cut(s) 513
MhlI GDGCHC 2 cut(s) 644, 979
MluCI AATT 7 cut(s) 513, 618, 777, 819, 873, 930, 1167
MlyI GAGTC 2 cut(s) 145, 1023
MnlI CCTC 8 cut(s) 89, 163, 229, 270, 327, 568, 891, 899
Mph1103I ATGCAT 2 cut(s) 180, 759
MseI TTAA 4 cut(s) 269, 425, 656, 818
MspA1I CMGCKG 2 cut(s) 338, 741
MspCI CTTAAG 1 cut(s) 424
MunI CAATTG 1 cut(s) 513
Mva1269I GAATGC 3 cut(s) 11, 308, 1108
MwoI GCNNNNNNNGC 4 cut(s) 507, 716, 1133, 1157
NcoI CCATGG 1 cut(s) 229
NdeII GATC 1 cut(s) 908
NlaIII CATG 7 cut(s) 178, 233, 372, 417, 553, 915, 1084
NlaIV GGNNCC 3 cut(s) 678, 898, 1161
NmuCI GTSAC 1 cut(s) 833
NsiI ATGCAT 2 cut(s) 180, 759
PceI AGGCCT 1 cut(s) 88
PciSI GCTCTTC 1 cut(s) 984
PctI GAATGC 3 cut(s) 11, 308, 1108
PfeI GAWTC 1 cut(s) 476
PflMI CCANNNNNTGG 1 cut(s) 670
PkrI GCNGC 8 cut(s) 35, 337, 340, 500, 523, 694, 740, 1135
PleI GAGTC 2 cut(s) 145, 1023
PpsI GAGTC 2 cut(s) 145, 1023
PspN4I GGNNCC 3 cut(s) 678, 898, 1161
PspPI GGNCC 3 cut(s) 701, 773, 1159
PstI CTGCAG 1 cut(s) 735
PvuII CAGCTG 1 cut(s) 338
RsaI GTAC 2 cut(s) 223, 505
RsaNI GTAC 2 cut(s) 222, 504
SapI GCTCTTC 1 cut(s) 984
SaqAI TTAA 4 cut(s) 269, 425, 656, 818
SatI GCNGC 8 cut(s) 34, 336, 339, 499, 522, 693, 739, 1134
Sau3AI GATC 1 cut(s) 908
Sau96I GGNCC 3 cut(s) 701, 773, 1159
SchI GAGTC 2 cut(s) 145, 1023
SduI GDGCHC 2 cut(s) 644, 979
SfaNI GCATC 4 cut(s) 14, 744, 766, 797
SfcI CTRYAG 4 cut(s) 324, 714, 731, 829
SinI GGWCC 2 cut(s) 701, 773
SmlI CTYRAG 1 cut(s) 424
SmoI CTYRAG 1 cut(s) 424
SpeI ACTAGT 1 cut(s) 1141
Sse9I AATT 7 cut(s) 513, 618, 777, 819, 873, 930, 1167
SseBI AGGCCT 1 cut(s) 88
SsiI CCGC 5 cut(s) 33, 212, 589, 741, 863
SspI AATATT 2 cut(s) 597, 1077
SspMI CTAG 2 cut(s) 950, 1142
StuI AGGCCT 1 cut(s) 88
StyI CCWWGG 3 cut(s) 229, 664, 1178
TaaI ACNGT 5 cut(s) 76, 226, 325, 942, 1097
TaqI TCGA 1 cut(s) 447
TasI AATT 7 cut(s) 513, 618, 777, 819, 873, 930, 1167
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 1 cut(s) 476
Tru1I TTAA 4 cut(s) 269, 425, 656, 818
Tru9I TTAA 4 cut(s) 269, 425, 656, 818
TscAI CASTG 4 cut(s) 328, 644, 775, 1026
TseFI GTSAC 1 cut(s) 833
TseI GCWGC 7 cut(s) 335, 338, 498, 521, 692, 738, 1133
Tsp45I GTSAC 1 cut(s) 833
TspDTI ATGAA 6 cut(s) 301, 354, 538, 830, 1069, 1182
TspRI CASTG 4 cut(s) 328, 644, 775, 1026
Van91I CCANNNNNTGG 1 cut(s) 670
Vha464I CTTAAG 1 cut(s) 424
VpaK11BI GGWCC 2 cut(s) 701, 773
XapI RAATTY 1 cut(s) 873
XspI CTAG 2 cut(s) 950, 1142
Zsp2I ATGCAT 2 cut(s) 180, 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.