FvH4_6g38480

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009173
Physical Location & Seq
Reverse (-)
30393474 .. 30415970
22497 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g38480.t1

Sequence Viewer

Length: 5562 bp
ATGTCTACCATCCCCGACACTCCAAAGAACACTTGGTTTGAGCACAGAATCAGTGAGGAGACTAGTATTGAAGGAATGGCGAGCTCGAGCGGGACCAAGGCGCCGAACTCGTCGGGGCGGCAGAGGTCGCTGTCGAGGGGGATGACGAGAATGGCTGCTAGAGTTGTGGACTTTCAGACGAAAGGCGACGATGCTTTGGACAGGGAAGTTGTGCCCTCCTGCCTTTCTTCTATTGCTCCCATTTTCAGGGTTGCCAATGAGATTGAGGAGGACAACCCTAGGGTTGCTTATCTATGCCGCTTCCATGGATTTCTAGAGGCACAAAAAATGGATCCACCGTCAAGTGGACAGGGAGTTCGTCAGTTTAAGACTCATCTTCTGCAGAGACTCGAAACGGACGAACGAGATACAAAACATCAGGTTGCTAAATCTGATACAAACGAAATCAAGTTGTACTACCACGAATTCTATGAAAAAAATATCAAAGATGGAGAATATACAAAGAAACCGGAAGAGATGGCCAGGAATTGTCAGATTGCAACTGTACTGTACGATGTGCTTAGGACAGTGGTACCTGCCAATCAGATAGACAAACAGAGCCAGAAAATTGCTGAAGACGTCAAGAGAAAAAGGGAGCAGTATGTAAACTATAACATTCTTCCGTTATATACTGTCGGGGTTAAACCAGCAGTTATGGAACTTCCTGAGATCAAAGCAGCGCTCCGTGCTCTACAGAATGTGGATGATCTTCCAATGCCTACAGGGAGGATTAAACCTGTGAATGATATACTTGATTGGCTTTCCGCTATTTTTGGGTTTCAGAAAGGAAATGTGGCAAATCAGAGGGAACATCTTGTTTTGCTACTTGCCAACATTGATGTAAGACATAGAAGTCTTGAGAGCTACACTGAGATTGCGAATGAGGTCTATGAAATTTTAAATAGCAATGCTCATCATGTTAGTGGAGAAACACACCAAACAGTAGCACGTGATGAAGAATCTTTTCTGAGGGAAGTTATATCTCCAATCTACGGGGTTTTGTTCGAGGAAGCAAGGAGAAACAAAATGGGCAAGGCAAGTCATTCAAGATGGAGAAATGATGATGATCTGAATGAATACTTCTGGTCGGACAAATGTTTTAAGCTAGGGTGGCCAATGGATCCTAAAGCAGATTTCTTTAGGCATTCGGATGAGATACAACCAGCGAATGAGAGGACAAATCAAGCTACTGGTGGTAGGAGGAAGCCTAAAACAAATTTTGTAGAAGTCCGAACCTTTTTGCACCTCTACAGAAGTTTTGATCGAATGTGGATATTCTTCATATTGGCTTTACAGGCTATGATTATTGTTGCATGGAGTCCTTCTGGATCTGTCACTTCAATATTTGATGCGGATGTCTTTAGAAGTGTGTTAAGCATCTTCATGACATACGCTTTCCTGAATTTATTACAAGCCACTTTGGACATGATTCTCAGTTGGAATGCATGGAAGAGCTTGGAATTCACTCAAATACTACGATACCTTTTGAAATTTGCAGTAGCTGTTGTCTGGGCTGTGGTTCTGCCTATTGGTTATTCAAGTTCTATGCAGAATCCTACAGGGCTTCTGAAATTCTTCAGTAATCAATCATTTTATAATTATGCTGTTGCAATCTATATGGTTCCTAATATATTGGCTGCTATGCTATTTGTTCTACCACCTTTACGGAGACACGTAGAGCGTTCCAACTGGCGCATTGTCACTCTTTTTATGTGGTGGGCTCAGCCAAAACTGTATGTCGGAAGAGGAATGCATGAGGATGTCTTCTCACTGCTGAAATATACATTGTTCTGGATCATGCTGCTAATCAGCAAGCTTGCATTCAGCTATTATGTGGAGATATCGCCGCTTGTTGCACCAACAAAGTTGATAATGGATATGCCCATCACTAATTATCAATGGCATGAGTTATTTCCTAATGCAACTCATAATATTGGTGTTGTCATTGCAATATGGGCTCCAATTGTCTTAGTATACATTATGGATGCACAAATATGGTATGCAATATTTTCTACTCTGTTCGGTGGGATTCAAGGAGCCTTCAGCCATTTGGGGGAGATACGAACACTTGGGATGTTGCGGTCCAGATTTAAGTCTGTGCCTTCAGCTTTCAGTGATCGGCTTATGCCATCATCAGAAATTAAGGATGCCAAAAAGAAGAAACACTTGGTGGACGAAGGACTTGTTCGAAGGAAAATTGCTAACTTCTCTCAGGTCTGGAATGAATTTATAAATTCTATGCGAATGGAAGACCTGATCAGCAATAGGGATAGAGATCTGCTGCTTGTACCGTATTCTTCAAGTGATGTTTCTGTTGTCCAGTGGCCCCCTTTCTTGCTTGCTAGCAAGGTTCCGATAGCATTAGACATGGCAAAGGATTTTACGGGGAAGTCGGATGAGGATTTATTCAAGAAGATTAGGAATGATGATTATATGTACTCGGCAGTAATTGAATGCTACGAGACAGTCAGGGATATTATACTTGGCCTTCTTGAACATGAGGCCGATAAGATGATTGTAAGGCAGATATGTTATGAAGTAGACAGCAGTATACAGCAGCAGAAGTTTTTGTTTAATTTCCGGATGAGTGGGCTGCCATTTCTTAGTGAGAGTTTGGAAAAGTTTCTTAAACTCTTGCTAGCTGGAGATGAGGATGTCGATAATTACTTGCGTCAGATAATCAATGTCCTTCAAGATATCATGGAGATTATAACACAGGAAGTAATGATCAATGGCCATCAAATTCTTGAAGCAGCTCACTACACAGAGGGTCAAAATGTCAAGAAGGAGCAAAGGTTTGAAAAAATCAACATATACCTTGCACAAAATCCATCTTGGAGAGAGAAGGTTGTTAGGCTTCATTTGCTTTTGACCGTCAAGGAATCTGCCATCAATGTGCCCGAAAACTTGGATGCTCGCAGGCGCATTACTTTCTTTGCGAACTCTCTATTCATGAACATGCCAAATGCTCCCAAAGTTCGTGACATGCTCTCCTTTAGTGTTCTGACTCCTTATTACAAAGAAGATGTTCTGTATTCTGATGATGAACTCAACAAGGAAAATGAGGATGGAATCTCAATCTTGTTCTACCTGCAGAAGATATATCCTGATGAATGGAGAAATTTCCAGGACCGCATAAATGAACCTAAGAATGAGTACTCTAACAAGGACAAGTCGGAGTTACTTCGCCAGTGGGTATCTTACAGGGGACAGACACTTTCTAGAACAGTGAGAGGAATGATGTACTATAGGAAGGCTCTAGACCTTCAATGTATCCTGGAAACAGCAGGAGATGGTGTTTATGGAGGCTACCCAGCCCAGATATTAAGTCAAATTGATGAGAAGGATATTCTTGATCGGGCACAAGCTCTGGCAGATTTGAAGTTCACTTATGTAGTTTCTTGTCAAATCTACGGTGAACAGAAGAATTCCAGTGAAAGTCGTGACAAAAGTTGTTACACAAATATTCTCAAGCTGATGTTAACGTATCCTTCTCTATGGGTTGCTTACATAGATACAAGAGAGGAGACAGTGAATGGAAAATCCCAAAAGATTTATTTTTCTGTGCTGGTCAAGGGAGGGGACAAATGGGATGAGGAAATATACCGCATCAAGCTTCCAGGTTCTCCTACAGAAATTGGTGAAGGCAGACCTGAAAATCAAAACCATGCCATCGTCTTTACTCGTGGAGAAGCCCTGCAAACCATTGACATGAATCAGGATAATTATTTTGAGGAAGCTTTCAAAATGAGAAATGTATTGGAGGAATTCCGGAAGCCTCGACGGGGGCAGCGGAAACCTACAATATTGGGTCTAAGGGAACATATTTTTACTGGAAGTGTTTCATCACTTGCTTCGTTCATGTCCAATCAAGAGACTAGTTTTGTCACTATAGGCCAACGAATTTTGGCAAATCTGAGGGTACGGTTTCATTATGGCCATCCGGATATTTTTGACAGACTCTTCCACATAACAAGGGGTGGCATAAGCAAAGCATCGAAAGTAATTAACTTAAGCGAGAATATATTTTCAGGGTACAATTCTGTTCTGCGTGGGGGATTTGTAACACATCATGAGTATATCCAAGTAGGCAAGGGGCGTGATGTTGGAATGAATCAGATATCACTTTTCGAGTCTAAAGTTGCAAATGGGAATGGAGAGCAGACACTTAGCCGTGATGTTTATAGGCTTGGCCATCGGTTTGATTTCTATAGAATGCTGTCCTTTTACTTCACAACAGTTGGGTTCTATTTTAGTAGCATGGTAACTGTGCTGACTGTCTATGTGTTTTTATATGGACGTCTATATATGGTTATGAGTGGATTGGAAAGTGAGATCCTGGATAGCCCAGCCATACATCAGACCAAGGCTTTTGAAGAAGCTCTGGCTACACAGTCTGTCTTTCAACTGGGGTTGTTGCTGGTCCTCCCCATGGTTATGGAAATTGGTCTGGAGAAAGGATTCCGCACTGCCTTGGGTGATTTCATCATCATGCAGTTGCAGCTGGCCTCTGTTTTCTTCACTTTCCAATTAGGAACAAAAGCACATTACTACGGAAGGACAATCTTACATGGTGGATCCAAATATAGACCAACTGGCCGTGGTTTTGTTGTTTTTCATGCAAAGTTTGCCGATAACTACAGGCTGTACTCAAGAAGTCACTTTGTGAAAGGGTTGGAGCTGTTGATTCTGCTGATTGTATATGGAGTCTACGGGAATGCTTATAGGAGTTCAAATCTGTATTTTTTCATCACGTTCTCCATGTGGTTTCTGGTTGCTTCCTGGTTGTTTGCTCCTTTTATCTTCAATCCATCCAGCTTTGACTGGCAAAAAACTGTAGATGATTGGGCGGATTGGAAGAGGTGGATGGGAAATCGTGGTGGTATTGGGATCTCACCTGACAAAAGTTGGGAATCATGGTGGGATGAAGAGCAGGAAGACCTCAAGCACACTACTATCCGTGGAAGAATTCTTGAGATAGTGCTTGCATGTCGCTTCTTCTTTTTCCAATATGGGATTGTTTACCACCTTGATATAGCTCATTTCAGCAAGAGTTCACTGGTTTATGGACTTTCTTGGGTGGTTATGGTAACAGTGCTTCTCGTCTTAAAGATGGTGTCAATGGTTAGACGAAGATTTGGTGCCGACTTTCAGCTCATGTTCAGAATTCTGAAAGCGCTTCTTTTCCTTGGCTTCATGTCAGTCATGACTGTCCTATTTGTAGTTTGTGGCCTTACTATTTCAGATCTGTTTGCCTCTATCCTTGCCTTCTTGCCCACAGGGTGGGCACTTCTTCTTATCGGGCAAGCATGCAGGCCAATGCTTAAGGGAGTAGGATTCTGGGAATCAATCAAGGAGCTGGGAAGAGAATATGATTATATAATGGGAGTGGCAGTCTTCGCGCCTGTTGCCATTTTGTCATGGTTCCCGTTTGTCTCCGAGTTCCAAACACGGTTGCTCTTCAACCAAGCATTCAGTCGGAGGGCTACAAATTTCAATGATTCTTGCCGGAAGGAAAGATAA

Protein Analysis

1854

Amino Acids

214.21

Weight (kDa)

8.35

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 77 - 204 4.7e-10 Vta1 like
FKS1_dom1 PF14288 302 - 385 4e-17 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 789 - 972 1.1e-67 Callose synthase 1 domain
Glucan_synthase PF02364 979 - 1069 1.8e-28 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1071 - 1671 1.7e-198 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1635, 2270, 2750
AasI GACNNNNNNGTC 1 cut(s) 891
AatII GACGTC 2 cut(s) 621, 4342
Acc36I ACCTGC 2 cut(s) 583, 3138
Acc65I GGTACC 1 cut(s) 571
AccB1I GGYRCC 3 cut(s) 100, 571, 5180
AccB7I CCANNNNNTGG 1 cut(s) 5322
AccBSI CCGCTC 1 cut(s) 90
AccI GTMKAC 5 cut(s) 5, 2013, 2580, 2590, 4749
AccII CGCG 1 cut(s) 5441
AccIII TCCGGA 3 cut(s) 2619, 3810, 3982
AcoI YGGCCR 6 cut(s) 519, 1151, 2773, 3976, 4231, 4636
AcuI CTGAAG 4 cut(s) 633, 1600, 2065, 2127
AcvI CACGTG 1 cut(s) 989
AcyI GRCGYC 3 cut(s) 101, 618, 4339
AdeI CACNNNGTG 3 cut(s) 2209, 4705, 5322
AfeI AGCGCT 2 cut(s) 720, 5217
AfiI CCNNNNNNNGG 7 cut(s) 246, 344, 1031, 2092, 3824, 4471, 5322
AflII CTTAAG 2 cut(s) 4051, 5363
AflIII ACRYGT 1 cut(s) 1711
AhlI ACTAGT 2 cut(s) 62, 3917
AjnI CCWGG 6 cut(s) 521, 3165, 3315, 3658, 4377, 4820
AjuI GAANNNNNNNTTGG 2 cut(s) 2189, 2221
AloI GAACNNNNNNTCC 2 cut(s) 339, 371
Alw21I GWGCWC 3 cut(s) 45, 86, 730
Alw26I GTCTC 7 cut(s) 53, 379, 1702, 2495, 3560, 3908, 5479
AlwNI CAGNNNCTG 1 cut(s) 1541
Ama87I CYCGRG 1 cut(s) 85
Aor13HI TCCGGA 3 cut(s) 2619, 3810, 3982
Aor51HI AGCGCT 2 cut(s) 720, 5217
ArsI GACNNNNNNTTYG 2 cut(s) 339, 371
Asp700I GAANNNNTTC 6 cut(s) 1002, 1613, 2661, 3066, 3880, 4499
Asp718I GGTACC 1 cut(s) 571
AspA2I CCTAGG 1 cut(s) 278
AspLEI GCGC 6 cut(s) 103, 721, 1734, 2964, 5218, 5443
AspS9I GGNCC 5 cut(s) 93, 2121, 2364, 3169, 4462
AsuHPI GGTGA 4 cut(s) 3467, 3692, 4529, 4926
AsuII TTCGAA 1 cut(s) 2227
AsuNHI GCTAGC 2 cut(s) 2381, 2677
AvaI CYCGRG 1 cut(s) 85
AvaII GGWCC 4 cut(s) 93, 2121, 3169, 4462
AvrII CCTAGG 1 cut(s) 278
BaeGI GKGCMC 4 cut(s) 216, 2940, 3403, 5329
BaeI ACNNNNGTAYC 2 cut(s) 555, 588
BalI TGGCCA 5 cut(s) 521, 1153, 2775, 3978, 4233
BamHI GGATCC 3 cut(s) 331, 1159, 4616
BanI GGYRCC 3 cut(s) 100, 571, 5180
BanII GRGCYC 3 cut(s) 86, 1762, 1999
BarI GAAGNNNNNNTAC 2 cut(s) 5121, 5153
BauI CACGAG 1 cut(s) 3723
BbrPI CACGTG 1 cut(s) 989
BbsI GAAGAC 5 cut(s) 621, 1795, 2295, 4983, 5428
Bbv12I GWGCWC 3 cut(s) 45, 86, 730
BceAI ACGGC 2 cut(s) 4197, 4623
BciT130I CCWGG 6 cut(s) 523, 3167, 3317, 3660, 4379, 4822
BciVI GTATCC 2 cut(s) 3323, 3537
BclI TGATCA 2 cut(s) 2295, 2766
BcoDI GTCTC 7 cut(s) 53, 379, 1702, 2495, 3560, 3908, 5479
BcuI ACTAGT 2 cut(s) 62, 3917
BfoI RGCGCY 3 cut(s) 104, 722, 5219
BfrI CTTAAG 2 cut(s) 4051, 5363
BfuAI ACCTGC 2 cut(s) 583, 3138
BfuI GTATCC 2 cut(s) 3323, 3537
BglII AGATCT 2 cut(s) 2314, 5284
BlnI CCTAGG 1 cut(s) 278
BlpI GCTNAGC 1 cut(s) 1761
BmcAI AGTACT 1 cut(s) 3197
Bme1390I CCNGG 6 cut(s) 523, 3167, 3317, 3660, 4379, 4822
Bme18I GGWCC 4 cut(s) 93, 2121, 3169, 4462
BmeT110I CYCGRG 1 cut(s) 85
BmgT120I GGNCC 5 cut(s) 93, 2121, 2364, 3169, 4462
BmrFI CCNGG 6 cut(s) 523, 3167, 3317, 3660, 4379, 4822
BmrI ACTGGG 1 cut(s) 4457
BmsI GCATC 8 cut(s) 181, 1378, 1425, 2014, 2176, 2941, 3657, 4043
BmtI GCTAGC 2 cut(s) 2385, 2681
BmuI ACTGGG 1 cut(s) 4457
BpiI GAAGAC 5 cut(s) 621, 1795, 2295, 4983, 5428
BpmI CTGGAG 2 cut(s) 2703, 4511
Bpu10I CCTNAGC 1 cut(s) 560
Bpu1102I GCTNAGC 1 cut(s) 1761
Bpu14I TTCGAA 1 cut(s) 2227
BpuEI CTTGAG 5 cut(s) 917, 3494, 4675, 4967, 5033
BsaAI YACGTR 2 cut(s) 989, 1714
BsaBI GATNNNNATC 2 cut(s) 1104, 2313
BsaHI GRCGYC 3 cut(s) 101, 618, 4339
BsaJI CCNNGG 9 cut(s) 96, 278, 304, 4404, 4470, 4512, 4639, 4999, 5227
BsaWI WCCGGW 4 cut(s) 508, 2619, 3810, 3982
BsaXI ACNNNNNCTCC 8 cut(s) 2819, 2849, 3014, 3044, 3321, 3351, 5418, 5448
Bsc4I CCNNNNNNNGG 7 cut(s) 246, 344, 1031, 2092, 3824, 4471, 5322
Bse3DI GCAATG 2 cut(s) 952, 1983
Bse8I GATNNNNATC 2 cut(s) 1104, 2313
BseAI TCCGGA 3 cut(s) 2619, 3810, 3982
BseBI CCWGG 6 cut(s) 523, 3167, 3317, 3660, 4379, 4822
BseDI CCNNGG 9 cut(s) 96, 278, 304, 4404, 4470, 4512, 4639, 4999, 5227
BseJI GATNNNNATC 2 cut(s) 1104, 2313
BseLI CCNNNNNNNGG 7 cut(s) 246, 344, 1031, 2092, 3824, 4471, 5322
BseMI GCAATG 2 cut(s) 952, 1983
BseMII CTCAG 7 cut(s) 696, 900, 998, 1486, 1775, 2264, 3947
BseRI GAGGAG 3 cut(s) 71, 281, 3578
BseSI GKGCMC 4 cut(s) 216, 2940, 3403, 5329
BseYI CCCAGC 3 cut(s) 3352, 4387, 5398
Bsh1236I CGCG 1 cut(s) 5441
BshNI GGYRCC 3 cut(s) 100, 571, 5180
BsiHKAI GWGCWC 3 cut(s) 45, 86, 730
BsiHKCI CYCGRG 1 cut(s) 85
BsiSI CCGG 5 cut(s) 509, 2620, 3811, 3983, 5548
BslFI GGGAC 3 cut(s) 106, 3261, 3635
BslI CCNNNNNNNGG 7 cut(s) 246, 344, 1031, 2092, 3824, 4471, 5322
BsmAI GTCTC 7 cut(s) 53, 379, 1702, 2495, 3560, 3908, 5479
BsmFI GGGAC 3 cut(s) 106, 3261, 3635
BsmI GAATGC 8 cut(s) 1183, 1486, 1794, 1859, 2498, 4260, 4762, 5510
BsoBI CYCGRG 1 cut(s) 85
Bsp119I TTCGAA 1 cut(s) 2227
Bsp1286I GDGCHC 9 cut(s) 45, 86, 216, 730, 1762, 1999, 2940, 3403, 5329
Bsp13I TCCGGA 3 cut(s) 2619, 3810, 3982
Bsp1720I GCTNAGC 1 cut(s) 1761
Bsp19I CCATGG 2 cut(s) 304, 4470
BspCNI CTCAG 7 cut(s) 697, 901, 999, 1485, 1774, 2263, 3948
BspEI TCCGGA 3 cut(s) 2619, 3810, 3982
BspFNI CGCG 1 cut(s) 5441
BspHI TCATGA 4 cut(s) 1422, 2991, 4111, 5244
BspMAI CTGCAG 2 cut(s) 384, 3135
BspMI ACCTGC 2 cut(s) 583, 3138
BspOI GCTAGC 2 cut(s) 2385, 2681
BspQI GCTCTTC 3 cut(s) 1484, 4962, 5504
BspT104I TTCGAA 1 cut(s) 2227
BspT107I GGYRCC 3 cut(s) 100, 571, 5180
BspTI CTTAAG 2 cut(s) 4051, 5363
BsrBI CCGCTC 1 cut(s) 90
BsrDI GCAATG 2 cut(s) 952, 1983
BssECI CCNNGG 9 cut(s) 96, 278, 304, 4404, 4470, 4512, 4639, 4999, 5227
BssNAI GTATAC 2 cut(s) 2014, 2591
BssNI GRCGYC 3 cut(s) 101, 618, 4339
BssSI CACGAG 1 cut(s) 3723
BssT1I CCWWGG 7 cut(s) 96, 278, 304, 4404, 4470, 4512, 5227
Bst1107I GTATAC 2 cut(s) 2014, 2591
Bst2BI CACGAG 1 cut(s) 3723
Bst2UI CCWGG 6 cut(s) 523, 3167, 3317, 3660, 4379, 4822
Bst6I CTCTTC 8 cut(s) 507, 1484, 1777, 4007, 4892, 4962, 5398, 5504
BstACI GRCGYC 3 cut(s) 101, 618, 4339
BstAFI CTTAAG 2 cut(s) 4051, 5363
BstAPI GCANNNNNTGC 1 cut(s) 4667
BstBAI YACGTR 2 cut(s) 989, 1714
BstBI TTCGAA 1 cut(s) 2227
BstDSI CCRYGG 4 cut(s) 304, 4470, 4639, 4999
BstFNI CGCG 1 cut(s) 5441
BstH2I RGCGCY 3 cut(s) 104, 722, 5219
BstHHI GCGC 6 cut(s) 103, 721, 1734, 2964, 5218, 5443
BstMAI GTCTC 7 cut(s) 53, 379, 1702, 2495, 3560, 3908, 5479
BstNI CCWGG 6 cut(s) 523, 3167, 3317, 3660, 4379, 4822
BstNSI RCATGY 4 cut(s) 3001, 3028, 5031, 5352
BstSCI CCNGG 6 cut(s) 521, 3165, 3315, 3658, 4377, 4820
BstSLI GKGCMC 4 cut(s) 216, 2940, 3403, 5329
BstUI CGCG 1 cut(s) 5441
BstV2I GAAGAC 5 cut(s) 621, 1795, 2295, 4983, 5428
BstX2I RGATCY 8 cut(s) 331, 1159, 1367, 2314, 4374, 4616, 4929, 5284
BstXI CCANNNNNNTGG 1 cut(s) 4477
BstYI RGATCY 8 cut(s) 331, 1159, 1367, 2314, 4374, 4616, 4929, 5284
BstZ17I GTATAC 2 cut(s) 2014, 2591
BsuI GTATCC 2 cut(s) 3323, 3537
BtgI CCRYGG 4 cut(s) 304, 4470, 4639, 4999
BtsI GCAGTG 2 cut(s) 1808, 4506
BveI ACCTGC 2 cut(s) 583, 3138
CaiI CAGNNNCTG 1 cut(s) 1541
CciI TCATGA 4 cut(s) 1422, 2991, 4111, 5244
CfoI GCGC 6 cut(s) 103, 721, 1734, 2964, 5218, 5443
Cfr13I GGNCC 5 cut(s) 93, 2121, 2364, 3169, 4462
CseI GACGC 1 cut(s) 2699
DinI GGCGCC 1 cut(s) 102
DraI TTTAAA 1 cut(s) 939
DraIII CACNNNGTG 3 cut(s) 2209, 4705, 5322
DrdI GACNNNNNNGTC 1 cut(s) 891
DseDI GACNNNNNNGTC 1 cut(s) 891
EaeI YGGCCR 6 cut(s) 519, 1151, 2773, 3976, 4231, 4636
Eam1104I CTCTTC 8 cut(s) 507, 1484, 1777, 4007, 4892, 4962, 5398, 5504
EarI CTCTTC 8 cut(s) 507, 1484, 1777, 4007, 4892, 4962, 5398, 5504
EciI GGCGGA 1 cut(s) 4904
Ecl136II GAGCTC 1 cut(s) 84
Eco130I CCWWGG 7 cut(s) 96, 278, 304, 4404, 4470, 4512, 5227
Eco24I GRGCYC 3 cut(s) 86, 1762, 1999
Eco32I GATATC 3 cut(s) 1881, 2737, 4161
Eco47I GGWCC 4 cut(s) 93, 2121, 3169, 4462
Eco47III AGCGCT 2 cut(s) 720, 5217
Eco53kI GAGCTC 1 cut(s) 84
Eco57I CTGAAG 4 cut(s) 633, 1600, 2065, 2127
Eco72I CACGTG 1 cut(s) 989
Eco88I CYCGRG 1 cut(s) 85
EcoICRI GAGCTC 1 cut(s) 84
EcoRI GAATTC 6 cut(s) 464, 1499, 3466, 3806, 5007, 5205
EcoRII CCWGG 6 cut(s) 521, 3165, 3315, 3658, 4377, 4820
EcoRV GATATC 3 cut(s) 1881, 2737, 4161
EcoT14I CCWWGG 7 cut(s) 96, 278, 304, 4404, 4470, 4512, 5227
EcoT22I ATGCAT 2 cut(s) 1486, 1794
EcoT38I GRGCYC 3 cut(s) 86, 1762, 1999
EgeI GGCGCC 1 cut(s) 102
EheI GGCGCC 1 cut(s) 102
ErhI CCWWGG 7 cut(s) 96, 278, 304, 4404, 4470, 4512, 5227
FalI AAGNNNNNCTT 4 cut(s) 2189, 2221, 5205, 5237
FaqI GGGAC 3 cut(s) 106, 3261, 3635
FauI CCCGC 1 cut(s) 83
FbaI TGATCA 2 cut(s) 2295, 2766
FblI GTMKAC 5 cut(s) 5, 2013, 2580, 2590, 4749
FriOI GRGCYC 3 cut(s) 86, 1762, 1999
GlaI GCGC 6 cut(s) 102, 720, 1733, 2963, 5217, 5442
GsaI CCCAGC 3 cut(s) 3356, 4391, 5402
GsuI CTGGAG 2 cut(s) 2703, 4511
HaeII RGCGCY 3 cut(s) 104, 722, 5219
HapII CCGG 5 cut(s) 509, 2620, 3811, 3983, 5548
HgaI GACGC 1 cut(s) 2699
HhaI GCGC 6 cut(s) 103, 721, 1734, 2964, 5218, 5443
Hin1I GRCGYC 3 cut(s) 101, 618, 4339
Hin6I GCGC 6 cut(s) 101, 719, 1732, 2962, 5216, 5441
HinP1I GCGC 6 cut(s) 101, 719, 1732, 2962, 5216, 5441
HincII GTYRAC 1 cut(s) 3522
HindII GTYRAC 1 cut(s) 3522
HindIII AAGCTT 3 cut(s) 1853, 3653, 3777
HpaI GTTAAC 1 cut(s) 3522
HpaII CCGG 5 cut(s) 509, 2620, 3811, 3983, 5548
HphI GGTGA 4 cut(s) 3467, 3692, 4529, 4926
Hpy99I CGWCG 3 cut(s) 115, 191, 3825
HpyCH4IV ACGT 6 cut(s) 618, 988, 1713, 3524, 4339, 4793
HpySE526I ACGT 6 cut(s) 618, 988, 1713, 3524, 4339, 4793
Hsp92I GRCGYC 3 cut(s) 101, 618, 4339
HspAI GCGC 6 cut(s) 101, 719, 1732, 2962, 5216, 5441
KasI GGCGCC 1 cut(s) 100
Kpn2I TCCGGA 3 cut(s) 2619, 3810, 3982
KpnI GGTACC 1 cut(s) 575
Ksp22I TGATCA 2 cut(s) 2295, 2766
KspAI GTTAAC 1 cut(s) 3522
LguI GCTCTTC 3 cut(s) 1484, 4962, 5504
LweI GCATC 8 cut(s) 181, 1378, 1425, 2014, 2176, 2941, 3657, 4043
MaeII ACGT 6 cut(s) 618, 988, 1713, 3524, 4339, 4793
MbiI CCGCTC 1 cut(s) 90
MfeI CAATTG 1 cut(s) 2001
MflI RGATCY 8 cut(s) 331, 1159, 1367, 2314, 4374, 4616, 4929, 5284
MhlI GDGCHC 9 cut(s) 45, 86, 216, 730, 1762, 1999, 2940, 3403, 5329
MlsI TGGCCA 5 cut(s) 521, 1153, 2775, 3978, 4233
MluNI TGGCCA 5 cut(s) 521, 1153, 2775, 3978, 4233
Mly113I GGCGCC 1 cut(s) 101
MlyI GAGTC 7 cut(s) 364, 381, 1366, 3040, 3993, 4181, 4755
MmeI TCCRAC 9 cut(s) 1107, 1457, 1749, 1759, 2412, 3195, 4126, 4695, 5498
Mox20I TGGCCA 5 cut(s) 521, 1153, 2775, 3978, 4233
Mph1103I ATGCAT 2 cut(s) 1486, 1794
MroI TCCGGA 3 cut(s) 2619, 3810, 3982
MroXI GAANNNNTTC 6 cut(s) 1002, 1613, 2661, 3066, 3880, 4499
MscI TGGCCA 5 cut(s) 521, 1153, 2775, 3978, 4233
Msp20I TGGCCA 5 cut(s) 521, 1153, 2775, 3978, 4233
MspA1I CMGCKG 2 cut(s) 3832, 4543
MspCI CTTAAG 2 cut(s) 4051, 5363
MspI CCGG 5 cut(s) 509, 2620, 3811, 3983, 5548
MspR9I CCNGG 6 cut(s) 523, 3167, 3317, 3660, 4379, 4822
MunI CAATTG 1 cut(s) 2001
Mva1269I GAATGC 8 cut(s) 1183, 1486, 1794, 1859, 2498, 4260, 4762, 5510
MvaI CCWGG 6 cut(s) 523, 3167, 3317, 3660, 4379, 4822
MvnI CGCG 1 cut(s) 5441
NarI GGCGCC 1 cut(s) 101
NcoI CCATGG 2 cut(s) 304, 4470
NheI GCTAGC 2 cut(s) 2381, 2677
NmeAIII GCCGAG 1 cut(s) 2459
NmuCI GTSAC 6 cut(s) 1372, 1738, 3020, 3482, 3925, 4697
NsiI ATGCAT 2 cut(s) 1486, 1794
NspI RCATGY 4 cut(s) 3001, 3028, 5031, 5352
NspV TTCGAA 1 cut(s) 2227
PaeI GCATGC 1 cut(s) 5352
PaeR7I CTCGAG 1 cut(s) 85
PagI TCATGA 4 cut(s) 1422, 2991, 4111, 5244
PciSI GCTCTTC 3 cut(s) 1484, 4962, 5504
PcsI WCGNNNNNNNCGW 1 cut(s) 396
PctI GAATGC 8 cut(s) 1183, 1486, 1794, 1859, 2498, 4260, 4762, 5510
PdmI GAANNNNTTC 6 cut(s) 1002, 1613, 2661, 3066, 3880, 4499
PflMI CCANNNNNTGG 1 cut(s) 5322
PfoI TCCNGGA 3 cut(s) 3165, 3315, 4377
PleI GAGTC 7 cut(s) 364, 381, 1365, 3040, 3993, 4180, 4754
PluTI GGCGCC 1 cut(s) 104
PmaCI CACGTG 1 cut(s) 989
PmlI CACGTG 1 cut(s) 989
PpsI GAGTC 7 cut(s) 364, 381, 1365, 3040, 3993, 4180, 4754
Ppu21I YACGTR 2 cut(s) 989, 1714
PsiI TTATAA 3 cut(s) 1635, 2270, 2750
Psp124BI GAGCTC 1 cut(s) 86
Psp6I CCWGG 6 cut(s) 521, 3165, 3315, 3658, 4377, 4820
PspCI CACGTG 1 cut(s) 989
PspFI CCCAGC 3 cut(s) 3352, 4387, 5398
PspGI CCWGG 6 cut(s) 521, 3165, 3315, 3658, 4377, 4820
PspPI GGNCC 5 cut(s) 93, 2121, 2364, 3169, 4462
PspXI VCTCGAGB 1 cut(s) 85
PstI CTGCAG 2 cut(s) 384, 3135
PstNI CAGNNNCTG 1 cut(s) 1541
PsuI RGATCY 8 cut(s) 331, 1159, 1367, 2314, 4374, 4616, 4929, 5284
PvuII CAGCTG 1 cut(s) 4543
SacI GAGCTC 1 cut(s) 86
SapI GCTCTTC 3 cut(s) 1484, 4962, 5504
Sau96I GGNCC 5 cut(s) 93, 2121, 2364, 3169, 4462
ScaI AGTACT 1 cut(s) 3197
SchI GAGTC 7 cut(s) 364, 381, 1366, 3040, 3993, 4181, 4755
ScrFI CCNGG 6 cut(s) 523, 3167, 3317, 3660, 4379, 4822
SduI GDGCHC 9 cut(s) 45, 86, 216, 730, 1762, 1999, 2940, 3403, 5329
SfaNI GCATC 8 cut(s) 181, 1378, 1425, 2014, 2176, 2941, 3657, 4043
SfoI GGCGCC 1 cut(s) 102
Sfr274I CTCGAG 1 cut(s) 85
SfuI TTCGAA 1 cut(s) 2227
SinI GGWCC 4 cut(s) 93, 2121, 3169, 4462
SlaI CTCGAG 1 cut(s) 85
SmlI CTYRAG 8 cut(s) 85, 896, 3509, 4051, 4690, 4982, 5012, 5363
SmoI CTYRAG 8 cut(s) 85, 896, 3509, 4051, 4690, 4982, 5012, 5363
SpeI ACTAGT 2 cut(s) 62, 3917
SphI GCATGC 1 cut(s) 5352
SspDI GGCGCC 1 cut(s) 100
SspI AATATT 5 cut(s) 1383, 1972, 2046, 3505, 3846
SstI GAGCTC 1 cut(s) 86
StyD4I CCNGG 6 cut(s) 521, 3165, 3315, 3658, 4377, 4820
StyI CCWWGG 7 cut(s) 96, 278, 304, 4404, 4470, 4512, 5227
TaiI ACGT 6 cut(s) 621, 991, 1716, 3527, 4342, 4796
TatI WGTACW 6 cut(s) 453, 544, 2475, 3195, 3282, 4686
TauI GCSGC 3 cut(s) 121, 300, 1888
TseFI GTSAC 6 cut(s) 1372, 1738, 3020, 3482, 3925, 4697
Tsp45I GTSAC 6 cut(s) 1372, 1738, 3020, 3482, 3925, 4697
TspGWI ACGGA 6 cut(s) 410, 651, 713, 1720, 4608, 4988
Van91I CCANNNNNTGG 1 cut(s) 5322
Vha464I CTTAAG 2 cut(s) 4051, 5363
VpaK11BI GGWCC 4 cut(s) 93, 2121, 3169, 4462
XbaI TCTAGA 3 cut(s) 313, 3260, 3298
XceI RCATGY 4 cut(s) 3001, 3028, 5031, 5352
XcmI CCANNNNNNNNNTGG 2 cut(s) 30, 4807
XhoI CTCGAG 1 cut(s) 85
XmaJI CCTAGG 1 cut(s) 278
XmiI GTMKAC 5 cut(s) 5, 2013, 2580, 2590, 4749
XmnI GAANNNNTTC 6 cut(s) 1002, 1613, 2661, 3066, 3880, 4499
ZraI GACGTC 2 cut(s) 619, 4340
ZrmI AGTACT 1 cut(s) 3197
Zsp2I ATGCAT 2 cut(s) 1486, 1794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.