Rh2AG489600

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0009173
Physical Location & Seq
Reverse (-)
71130574 .. 71146907
16334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG489600.1

Sequence Viewer

Length: 5574 bp
ATGGCGAGCTCCAGCGGGACCAAGGCCGATTCGCGGCCGCAGAGGTCGCTGTCGAGGGGAATGACGAGAATGGCTACTAGAGTTGTGGACTTTCCGACGGAGGGCGACGATGCGTTGGACAGTGGAGTTGTGCCGTCGTGCCTTGCGTCTATTGCTCCGATTTTCCGGGTTGCCAATGAGATTGAGAAGGACAACCCTAGGGTTGCTTATCTCTGCCGCTTCCATGGATTTGAAAAGGCACATACCATGGATCCAACATCAAGTGGACGTGGAGTTCGTCAGTTTAAGACGCATCTTTTGCACAGACTCGAAAGGGACGAAGTAGATACAAAACATCAGCTTGCTAAATCTGATACAAAAGAAATCATGTTGTATTACCACCAATTCTATGAGAAAAATATCAGAGATGGAGAATATACAAAGAAACCGGAAGAGATGGCCAGGATTTGTCAGATTGCAACTGTACTGTATGATGTGCTCAAGACAGTGGTACCTGCCTCTCAAATAGACATACAGACCCAGAGAATTGCTGAAGATGTCAAGAGCAAAAGGGAGCAATATGTAAACTATAACATTCTTCCATTATATACTGTCGGGGTTAAACCGACAATTATGGAACTTCCTGAGATCAAAGCAGCGCTCCGTGCTCTACAGAATGTGAGTAATCTTCCAATGCCAAGAATGCATTTTAAACCCACGAATCCTGATGACAAGTCGACAATGCCTACAGAGAGGATTAAACCTGTGAATGACATACTCGATTGGCTTTCGGCTATTTTTGGGTTTCAGAAAGGAAATGTGGCAAATCAGAGGGAACATTTAATTTTGCTGCTTGCCAACATTGATGTAAGACATAGAAGTCCTGAGAACTACGATGAGATGGCGAACGAGGTCTATGGAATTTTAAATAGCAATGCTCATACTGTTAGTGGAGAAACATACCAAACAGTTGTACGTGATGAAGAATCTTTTTTGAGGGATGTTATAACTCCTATCTACCAGGTTTTGTACAAGGAAGCAAAGAGAAACAAAATGGGCAAGGCAAGTCATTCAAGATGGAGAAATTATGATGATTTGAATGAATACTTCTGGTCGGACAAATGTTTTAAGCTAGGGTGGCCAATGGATGCTAAAGCAGATTTCTTTAGGCATTCAGATGGGATACAACCAGTAAATGAGAGGACCAATCAAGCTACTGGTGGTAGGAGGAAGCCTAAAACAAATTTTGTAGAAGTCCGAACATTTTTACACCTCTACAGAAGTTTTGATCGAATGTGGATATTCTTCATATTGGCTTTACAGGCTATGATTATTATTGCATGGAGTCCTTCTGGATCTATCACTTCAATATTTGATGCAGACGTCTTCAGAAGTGTTTTAAGCATCTTCATGACATATGCTTTCCTTAATTTATTACAAGCCACTTTGGACATAATTCTCAGTTGGAATGCATGGAAGAGCTTGAAAGTCACTCAAATATTACGATACCTCTTGAAATTTGCAGTAGCTGTTGTCTGGGCTGTGGTTCTGCCTATTGGTTATTCTAGTTCTATGCAAAATCCTACAGGGCTTCTGAAATTCTTCAGTAGTTGGAGTCGGGATTGGCGGAATCAGTCATTTTATAATTATGCTGTTGCAATCTATATGGTTCCTAATTTATTGGCTGCTATGCTATTTTTTCTTCCACCTTTACGAAGACACATAGAGCGTTCCAACTGGCGCATTGTCACTCTTTTTATGTGGTGGGCTCAGCCAAAACTGTATGTCGGAAGAGGAATGCATGAGGATGTCTTCTCACTGCTGAAATATACATTGTTCTGGATCATGCTGCTAATTAGCAAGCTAGCGTTCAGCTATTATGTGGAGATATTGCCGCTTGTTGCACCAACAAAGTTGATAATGGATATGCCTATCAGTAATTACCAATGGCATGAGTTCTTTCCGAATGCAACTCATAATATTGGTGTTGTCATTGCAATATGGACTCCAATTATCCTGGTATATTTTATGGATGCACAAATATGGTATGCAATATTTTCTACTCTGTTTGGTGGGATTCATGGAGCCTTCAGCCATTTGGGGGAGATACGAACACTTGGGATGTTGCGGTCCAGATTTGAGTCTGTGCCTTCAGCTTTCAGTGATCGGCTTATGCCATCGTCAGAGAATAAGGATGCCAGAAAGAAGAAACACTTGGTGGATGAAGGACTTGTTCGAAAGAACATTGCTAATTTCTCTCAGGTCTGGAATGAATTTATAAATTCTATGCGAATGGAAGACCTGATCAGCAATAGGGATAGGGATCTGCTGCTTGTTCCGTATTCTTCAAGTGATGTTTCTGTTGTCCAGTGGCCTCCTTTCTTGCTTGCTAGCAAGATTCCAATAGCATTAGACATGGCAAAGGATTTTACAGGAAAGTCGGATGGTGATTTATTCAGGAAGATTAAGAATGATGATTATATGTACTCGGCAGTGATTGAATGCTATGAGACACTCAGGGATATTATATTTGGCCTTCTTGATGATGACGCCGATAAGATGATTGTAAGGCAGATATGTTATGAAGTAGACAGTAGTATACAGCAGCAGAGGTTTTTGAATAATTTCCGTATGAGTGGGCTGCCATTTCTTAGTGAGAGGTTGGAGAAGTTTCTCAAACTCTTGCTAGCCGAAGATGAGAATGTCGAAAATTCCTTGCGTCAGATAATCAATGTCCTTCAAGATATCATGGAGATTATAACACAGGATGTAATGATCAATGGCCATGAAATCCTCGAAGCAGCTCACTACATAGAGGGTCAAAATGTCAAGAAGGAGCAAAGGTTTCAAAAAATCAACATATATCTTGCCCAAAATCCATCTTGGAGAGAGAAGGTCGTTAGGCTTCATTTGCTTTTGACCGTCAAGGAATCTGCCATCAATGTGCCCCAAAACTTGGATGCTCGCAGGCGCATTACTTTCTTTGCGAACTCCTTATTCATGAACATGCCAAATGCTCCCAAAGTTCGTGACATGCTCTCCTTTAGTGTTCTGACTCCTTATTACAAAGAAGATGTTCTTTATTCTGATGAGGAACTTACCAAGGAAAATGAGGATGGAATCTCAATTTTGTTCTACCTGCAGAAGATATATCCTGATGAATGGACAAATTTCCTGGACCGCATAAATGAACATAAAAGTCAGTACACTGAAAAGGACAAGTCGGAGTTAATTCGTCAGTGGGTATCTTACAGGGGACAGACACTTTCTAGAACAGTGAGAGGGATGATGTACTATAGGAAGGCTCTAGACCTTCAATGTATCCTGGAAACAGCAGGAGATGGTGGTGTTTATGGAGGCGACGCAGCCCATATTTTAAGTGATAAAGATGAGAAGGATATTCTTGATCGGGCACAAGCTCTGGCAGATTTGAAGTTCACTTATGTAGTTTCTTGTCAGGTCTATGGTGAACAGAAGAATTCCACTGAAGCCCGAGACAAAAGTTGTTACACTAATATTCTCAAGCTGATGTTAACGTATCCATCTCTACGTGTTGCTTACATAGATACAAGAGAGAAGACAGTGAATGGAAAATCCCAAAAGATTCATTTCTCTGTTCTGGTCAAGGGAGGTGACAAATGGGATGAGGAAATATACCGCATCAAGCTTCCAGGTCCTCCAACAGCAATTGGTGAAGGAAAACCCGAAAATCAAAACCATGCCATCGTATTTACTCGTGGAGAAGCCCTGCAAACCATTGACATGAATCAGGATAATTATTTTGAGGAAGCTTTCAAAATGAGAAATGTATTGGAGGAATTTCTAAAGCCTCGACGGGGGCAGCGGAAACCTACAATATTGGGTCTAAGGGAACATATATTTACCGGAAGTGTTTCATCACTCGCTTGGTTCATGTCCAATCAAGAGACAAGTTTTGTGACTATAGGACAACGAATTTTGGCAAATCCGCTGAGGGTACGGTTTCATTATGGCCATCCTGATATTTTTGACAGAATCTTTCACATAACAAGAGGCGGCATAAGCAAAGCATCAAAAGTAATTAACTTAAGCGAGGATATATTTTCAGGATATAATTCTGTTCTGCGTGGGGGATTTATAACACATCATGAGTATATCCAAGTAGGCAAGGGGCGTGATGTTGGAATGAATCAAATATCGCTTTTCGAGGCTAAAGTTGCAAATGGAAATGGAGAGCAGACACTTAGCCGTGATGTTTATAGGCTTGGGCGTCGGTTTGATTTCTATAGAATGCTGTCTTTTTACTTCACAACAGTTGGGTTCTATTTTAGTAGTATGGTAACTGTGCTGACTGTCTATGTGTTTTTATACGGACGTCTATATATGGTTATGAGTGGATTGGAAAGTGAGATCCTGGATAGCCCAGCCATACATCAGACCAAGGCTTTTGAACAAGCTCTGGCTACACAGTCTGTCTTTCAACTGGGCTTGTTGCTGGTCCTTCCCATGGTTATGGAAATTGGTCTGGAGAAAGGATTTCGCACTGCCTTGGGTGATTTCATCATCATGCAGCTGCAGCTGGCCTCTGTTTTCTTCACTTTCCAACTAGGAACAAAAGCACATTACTACGGAAGGACAATCTTACATGGAGGATCTAAATATCGAGCAACTGGCCGTGGTTTTGTTGTTTTTCATGCAAAGTTTGCCGATAACTACAGGCTGTACTCACGAAGTCACTTTGTGAAAGGGTTGGAGCTGTTGATTCTGCTGATTGTGTATGGAGTCTATGGGAATGCATATAGGAGTTCAAATCTGTATTTTTTCATCACGTTCTCCATGTGGTTTCTGGTTGCTTCCTGGTTGTTTGCTCCTTTTATCTTTAATCCGTCCAGCTTTGACTGGCAAAAAACTGTAGATGATTGGACGGATTGGAAGAGGTGGATGGGAAATCGTGGTGGTATTGGGATCTCACCTGACAAAAGTTGGGAATCATGGTGGGATGAAGAACAGGAACACCTCAAGCACACTATTATCCGTGGAAGAATTATTGAGATAATTCTTGCATGTCGCTTCTTTTTTTACCAATATGGGATTGTTTACCACCTCGATATAGCTCATTTCAGCAAGAGTTTACTGGTTTATGGACTTTCTTGGGTGGTTATGGTAACGGTGCTCCTCGTCTTAAAGATGGTGTCAATGGGTAGACGAAGATTTGGCACTGACTTTCAGCTCATGTTCAGAATTCTGAAGGCGCTTCTTTTTCTTGGCTTCATGTCAGTCATGACTGTCCTATTTGTAGTTTGTGGCCTTACTATTTCTGATCTATTTGCTGCTATTCTTGCCTTCTTGCCCACAGGGTGGGCACTACTTCTTATCGGGCAAGTATGCAGGCCAATGGTTAAGGGAGTAGGATTCTGGGAATCAATTAAGGAGCTGGGAAGAGCCTATGATTACATAATGGGAGTGGTAGTCTTCATGCCGGTTGCCATTTTGTCATGGTTCCCATTTGTCTCAGAGTTCCAGACACGGTTGCTCTTCAACCAAGCATTCAGTCGAGGCCTACAAATTTCAATGATTCTCGCCGGGAGGAAAGATAAGACAGCTTCTAATAGATAG

Protein Analysis

1857

Amino Acids

214.72

Weight (kDa)

8.97

Isoelectric Point (pI)

39.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 50 - 180 1.8e-10 Vta1 like
FKS1_dom1 PF14288 291 - 374 6.7e-21 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 785 - 968 3e-70 Callose synthase 1 domain
Glucan_synthase PF02364 975 - 1065 1.3e-29 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1068 - 1669 4.3e-201 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 986, 1623, 2258, 2738, 4097
AasI GACNNNNNNGTC 1 cut(s) 858
AatII GACGTC 2 cut(s) 1365, 4336
Acc36I ACCTGC 2 cut(s) 502, 3126
Acc65I GGTACC 1 cut(s) 490
AccB1I GGYRCC 1 cut(s) 490
AccB7I CCANNNNNTGG 2 cut(s) 2935, 5316
AccI GTMKAC 4 cut(s) 716, 2568, 2578, 5161
AccII CGCG 1 cut(s) 34
AclWI GGATC 8 cut(s) 245, 258, 1342, 1829, 2310, 4363, 4618, 4931
AcoI YGGCCR 6 cut(s) 35, 438, 1118, 2761, 3970, 4630
AcuI CTGAAG 7 cut(s) 552, 1351, 1567, 2053, 2115, 3486, 5225
AcyI GRCGYC 4 cut(s) 1362, 2529, 4228, 4333
AdeI CACNNNGTG 3 cut(s) 2197, 4699, 5316
AfaI GTAC 9 cut(s) 465, 492, 954, 1010, 2465, 3185, 3272, 3957, 4682
AfeI AGCGCT 1 cut(s) 639
AfiI CCNNNNNNNGG 7 cut(s) 33, 101, 2080, 2935, 3815, 4465, 5316
AflII CTTAAG 1 cut(s) 4045
AflIII ACRYGT 1 cut(s) 3529
AjiI CACGTC 1 cut(s) 269
AjnI CCWGG 8 cut(s) 440, 999, 1995, 3153, 3303, 3649, 4371, 4814
AjuI GAANNNNNNNTTGG 2 cut(s) 2177, 2209
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
Alw21I GWGCWC 4 cut(s) 11, 480, 649, 5133
Alw26I GTCTC 4 cut(s) 2483, 3468, 3899, 5473
AlwI GGATC 8 cut(s) 245, 258, 1342, 1829, 2310, 4363, 4618, 4931
AlwNI CAGNNNCTG 1 cut(s) 1508
Ama87I CYCGRG 1 cut(s) 3471
Aor51HI AGCGCT 1 cut(s) 639
ArsI GACNNNNNNTTYG 4 cut(s) 258, 280, 290, 312
Asp700I GAANNNNTTC 4 cut(s) 1580, 2603, 3054, 3871
Asp718I GGTACC 1 cut(s) 490
AspA2I CCTAGG 1 cut(s) 197
AspLEI GCGC 4 cut(s) 640, 1722, 2952, 5212
AspS9I GGNCC 6 cut(s) 18, 1182, 2109, 3157, 3653, 4456
AsuC2I CCSGG 2 cut(s) 167, 5542
AsuHPI GGTGA 6 cut(s) 2438, 3458, 3623, 3683, 4523, 4920
AsuII TTCGAA 1 cut(s) 2215
AsuNHI GCTAGC 3 cut(s) 1843, 2369, 2665
AvaI CYCGRG 1 cut(s) 3471
AvaII GGWCC 6 cut(s) 18, 1182, 2109, 3157, 3653, 4456
AvrII CCTAGG 1 cut(s) 197
BaeGI GKGCMC 3 cut(s) 2928, 3394, 5323
BaeI ACNNNNGTAYC 2 cut(s) 474, 507
BalI TGGCCA 4 cut(s) 440, 1120, 2763, 3972
BamHI GGATCC 1 cut(s) 250
BanI GGYRCC 1 cut(s) 490
BanII GRGCYC 2 cut(s) 11, 1750
BauI CACGAG 1 cut(s) 3714
BbsI GAAGAC 6 cut(s) 1357, 1702, 1783, 2283, 3563, 5422
Bbv12I GWGCWC 4 cut(s) 11, 480, 649, 5133
BbvCI CCTCAGC 1 cut(s) 3950
BceAI ACGGC 3 cut(s) 118, 4191, 4617
BciT130I CCWGG 8 cut(s) 442, 1001, 1997, 3155, 3305, 3651, 4373, 4816
BciVI GTATCC 3 cut(s) 1155, 3311, 3528
BclI TGATCA 2 cut(s) 2283, 2754
BcnI CCSGG 2 cut(s) 167, 5542
BcoDI GTCTC 4 cut(s) 2483, 3468, 3899, 5473
BfoI RGCGCY 2 cut(s) 641, 5213
BfrI CTTAAG 1 cut(s) 4045
BfuAI ACCTGC 2 cut(s) 502, 3126
BfuI GTATCC 3 cut(s) 1155, 3311, 3528
BlnI CCTAGG 1 cut(s) 197
BlpI GCTNAGC 1 cut(s) 1749
Bme18I GGWCC 6 cut(s) 18, 1182, 2109, 3157, 3653, 4456
BmeT110I CYCGRG 1 cut(s) 3471
BmgBI CACGTC 1 cut(s) 269
BmgT120I GGNCC 6 cut(s) 18, 1182, 2109, 3157, 3653, 4456
BmiI GGNNCC 6 cut(s) 19, 252, 492, 1650, 2065, 5459
BmrI ACTGGG 1 cut(s) 4451
BmtI GCTAGC 3 cut(s) 1847, 2373, 2669
BmuI ACTGGG 1 cut(s) 4451
BpiI GAAGAC 6 cut(s) 1357, 1702, 1783, 2283, 3563, 5422
BpmI CTGGAG 1 cut(s) 4505
Bpu10I CCTNAGC 1 cut(s) 3950
Bpu1102I GCTNAGC 1 cut(s) 1749
Bpu14I TTCGAA 1 cut(s) 2215
BpuEI CTTGAG 3 cut(s) 464, 3485, 4961
BpuMI CCSGG 2 cut(s) 167, 5542
BsaAI YACGTR 2 cut(s) 956, 3530
BsaBI GATNNNNATC 1 cut(s) 2301
BsaHI GRCGYC 4 cut(s) 1362, 2529, 4228, 4333
BsaWI WCCGGW 2 cut(s) 427, 3863
BsaXI ACNNNNNCTCC 6 cut(s) 2807, 2837, 3002, 3032, 5412, 5442
Bsc4I CCNNNNNNNGG 7 cut(s) 33, 101, 2080, 2935, 3815, 4465, 5316
Bse118I RCCGGY 1 cut(s) 5437
Bse1I ACTGG 8 cut(s) 1169, 1201, 1721, 2347, 4446, 4633, 4862, 5097
Bse3DI GCAATG 3 cut(s) 919, 1971, 2223
Bse8I GATNNNNATC 1 cut(s) 2301
BseBI CCWGG 8 cut(s) 442, 1001, 1997, 3155, 3305, 3651, 4373, 4816
BseJI GATNNNNATC 1 cut(s) 2301
BseLI CCNNNNNNNGG 7 cut(s) 33, 101, 2080, 2935, 3815, 4465, 5316
BseMI GCAATG 3 cut(s) 919, 1971, 2223
BseMII CTCAG 8 cut(s) 615, 855, 1453, 1763, 2252, 2509, 3941, 5484
BseNI ACTGG 8 cut(s) 1169, 1201, 1721, 2347, 4446, 4633, 4862, 5097
BseRI GAGGAG 1 cut(s) 5123
BseSI GKGCMC 3 cut(s) 2928, 3394, 5323
BseX3I CGGCCG 1 cut(s) 35
BseYI CCCAGC 2 cut(s) 4381, 5392
Bsh1236I CGCG 1 cut(s) 34
Bsh1285I CGRYCG 1 cut(s) 38
BshNI GGYRCC 1 cut(s) 490
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 4 cut(s) 11, 480, 649, 5133
BsiHKCI CYCGRG 1 cut(s) 3471
BsiSI CCGG 5 cut(s) 166, 428, 3864, 5438, 5541
BslFI GGGAC 3 cut(s) 31, 329, 3249
BslI CCNNNNNNNGG 7 cut(s) 33, 101, 2080, 2935, 3815, 4465, 5316
BsmAI GTCTC 4 cut(s) 2483, 3468, 3899, 5473
BsmFI GGGAC 3 cut(s) 31, 329, 3249
BsmI GAATGC 9 cut(s) 687, 1150, 1453, 1782, 1951, 2486, 4254, 4756, 5504
BsoBI CYCGRG 1 cut(s) 3471
Bsp119I TTCGAA 1 cut(s) 2215
Bsp1286I GDGCHC 8 cut(s) 11, 480, 649, 1750, 2928, 3394, 5133, 5323
Bsp1407I TGTACA 1 cut(s) 1008
Bsp1720I GCTNAGC 1 cut(s) 1749
Bsp19I CCATGG 3 cut(s) 223, 246, 4464
BspCNI CTCAG 8 cut(s) 616, 856, 1452, 1762, 2251, 2508, 3942, 5483
BspFNI CGCG 1 cut(s) 34
BspHI TCATGA 4 cut(s) 1389, 2979, 4105, 5238
BspLI GGNNCC 6 cut(s) 19, 252, 492, 1650, 2065, 5459
BspMAI CTGCAG 2 cut(s) 3123, 4536
BspMI ACCTGC 2 cut(s) 502, 3126
BspOI GCTAGC 3 cut(s) 1847, 2373, 2669
BspPI GGATC 8 cut(s) 245, 258, 1342, 1829, 2310, 4363, 4618, 4931
BspQI GCTCTTC 3 cut(s) 1451, 5392, 5498
BspT104I TTCGAA 1 cut(s) 2215
BspT107I GGYRCC 1 cut(s) 490
BspTI CTTAAG 1 cut(s) 4045
BsrDI GCAATG 3 cut(s) 919, 1971, 2223
BsrFI RCCGGY 1 cut(s) 5437
BsrGI TGTACA 1 cut(s) 1008
BsrI ACTGG 8 cut(s) 1169, 1201, 1721, 2347, 4446, 4633, 4862, 5097
BssAI RCCGGY 1 cut(s) 5437
BssNAI GTATAC 1 cut(s) 2579
BssNI GRCGYC 4 cut(s) 1362, 2529, 4228, 4333
BssSI CACGAG 1 cut(s) 3714
BssT1I CCWWGG 8 cut(s) 21, 197, 223, 246, 3081, 4398, 4464, 4506
Bst1107I GTATAC 1 cut(s) 2579
Bst2BI CACGAG 1 cut(s) 3714
Bst2UI CCWGG 8 cut(s) 442, 1001, 1997, 3155, 3305, 3651, 4373, 4816
Bst6I CTCTTC 6 cut(s) 426, 1451, 1765, 4886, 5392, 5498
BstACI GRCGYC 4 cut(s) 1362, 2529, 4228, 4333
BstAFI CTTAAG 1 cut(s) 4045
BstAPI GCANNNNNTGC 2 cut(s) 298, 4661
BstAUI TGTACA 1 cut(s) 1008
BstBAI YACGTR 2 cut(s) 956, 3530
BstBI TTCGAA 1 cut(s) 2215
BstDSI CCRYGG 5 cut(s) 223, 246, 4464, 4633, 4993
BstFNI CGCG 1 cut(s) 34
BstH2I RGCGCY 2 cut(s) 641, 5213
BstHHI GCGC 4 cut(s) 640, 1722, 2952, 5212
BstMAI GTCTC 4 cut(s) 2483, 3468, 3899, 5473
BstMCI CGRYCG 1 cut(s) 38
BstNI CCWGG 8 cut(s) 442, 1001, 1997, 3155, 3305, 3651, 4373, 4816
BstNSI RCATGY 3 cut(s) 2989, 3016, 5025
BstSLI GKGCMC 3 cut(s) 2928, 3394, 5323
BstUI CGCG 1 cut(s) 34
BstV2I GAAGAC 6 cut(s) 1357, 1702, 1783, 2283, 3563, 5422
BstX2I RGATCY 6 cut(s) 250, 1334, 2302, 4368, 4610, 4923
BstXI CCANNNNNNTGG 1 cut(s) 4471
BstYI RGATCY 6 cut(s) 250, 1334, 2302, 4368, 4610, 4923
BstZ17I GTATAC 1 cut(s) 2579
BstZI CGGCCG 1 cut(s) 35
BsuI GTATCC 3 cut(s) 1155, 3311, 3528
BtgI CCRYGG 5 cut(s) 223, 246, 4464, 4633, 4993
BtrI CACGTC 1 cut(s) 269
BtsI GCAGTG 3 cut(s) 1796, 2478, 4500
BveI ACCTGC 2 cut(s) 502, 3126
CaiI CAGNNNCTG 1 cut(s) 1508
CciI TCATGA 4 cut(s) 1389, 2979, 4105, 5238
CciNI GCGGCCGC 1 cut(s) 35
CfoI GCGC 4 cut(s) 640, 1722, 2952, 5212
Cfr10I RCCGGY 1 cut(s) 5437
Cfr13I GGNCC 6 cut(s) 18, 1182, 2109, 3157, 3653, 4456
CseI GACGC 6 cut(s) 135, 298, 2537, 2687, 3350, 4217
CsiI ACCWGGT 1 cut(s) 999
Csp6I GTAC 9 cut(s) 464, 491, 953, 1009, 2464, 3184, 3271, 3956, 4681
CviQI GTAC 9 cut(s) 464, 491, 953, 1009, 2464, 3184, 3271, 3956, 4681
DraI TTTAAA 2 cut(s) 691, 906
DraIII CACNNNGTG 3 cut(s) 2197, 4699, 5316
DrdI GACNNNNNNGTC 1 cut(s) 858
DseDI GACNNNNNNGTC 1 cut(s) 858
EaeI YGGCCR 6 cut(s) 35, 438, 1118, 2761, 3970, 4630
EagI CGGCCG 1 cut(s) 35
Eam1104I CTCTTC 6 cut(s) 426, 1451, 1765, 4886, 5392, 5498
EarI CTCTTC 6 cut(s) 426, 1451, 1765, 4886, 5392, 5498
EciI GGCGGA 1 cut(s) 1621
Ecl136II GAGCTC 1 cut(s) 9
EclXI CGGCCG 1 cut(s) 35
Eco130I CCWWGG 8 cut(s) 21, 197, 223, 246, 3081, 4398, 4464, 4506
Eco147I AGGCCT 1 cut(s) 5517
Eco24I GRGCYC 2 cut(s) 11, 1750
Eco32I GATATC 1 cut(s) 2725
Eco47I GGWCC 6 cut(s) 18, 1182, 2109, 3157, 3653, 4456
Eco47III AGCGCT 1 cut(s) 639
Eco52I CGGCCG 1 cut(s) 35
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 7 cut(s) 552, 1351, 1567, 2053, 2115, 3486, 5225
Eco88I CYCGRG 1 cut(s) 3471
EcoICRI GAGCTC 1 cut(s) 9
EcoO109I RGGNCCY 1 cut(s) 3653
EcoRI GAATTC 2 cut(s) 3457, 5199
EcoRII CCWGG 8 cut(s) 440, 999, 1995, 3153, 3303, 3649, 4371, 4814
EcoRV GATATC 1 cut(s) 2725
EcoT14I CCWWGG 8 cut(s) 21, 197, 223, 246, 3081, 4398, 4464, 4506
EcoT22I ATGCAT 4 cut(s) 687, 1453, 1782, 4756
EcoT38I GRGCYC 2 cut(s) 11, 1750
ErhI CCWWGG 8 cut(s) 21, 197, 223, 246, 3081, 4398, 4464, 4506
FalI AAGNNNNNCTT 4 cut(s) 2177, 2209, 3042, 3074
FaqI GGGAC 3 cut(s) 31, 329, 3249
FauI CCCGC 1 cut(s) 8
FauNDI CATATG 1 cut(s) 1396
FbaI TGATCA 2 cut(s) 2283, 2754
FblI GTMKAC 4 cut(s) 716, 2568, 2578, 5161
FriOI GRGCYC 2 cut(s) 11, 1750
GlaI GCGC 4 cut(s) 639, 1721, 2951, 5211
GsaI CCCAGC 2 cut(s) 4385, 5396
GsuI CTGGAG 1 cut(s) 4505
HaeII RGCGCY 2 cut(s) 641, 5213
HapII CCGG 5 cut(s) 166, 428, 3864, 5438, 5541
HgaI GACGC 6 cut(s) 135, 298, 2537, 2687, 3350, 4217
HhaI GCGC 4 cut(s) 640, 1722, 2952, 5212
Hin1I GRCGYC 4 cut(s) 1362, 2529, 4228, 4333
Hin6I GCGC 4 cut(s) 638, 1720, 2950, 5210
HinP1I GCGC 4 cut(s) 638, 1720, 2950, 5210
HincII GTYRAC 2 cut(s) 717, 3513
HindII GTYRAC 2 cut(s) 717, 3513
HindIII AAGCTT 2 cut(s) 3644, 3768
HpaI GTTAAC 1 cut(s) 3513
HpaII CCGG 5 cut(s) 166, 428, 3864, 5438, 5541
HphI GGTGA 6 cut(s) 2438, 3458, 3623, 3683, 4523, 4920
Hpy99I CGWCG 6 cut(s) 100, 110, 139, 3344, 3816, 4233
HpyCH4IV ACGT 7 cut(s) 268, 955, 1362, 3515, 3529, 4333, 4787
HpySE526I ACGT 7 cut(s) 268, 955, 1362, 3515, 3529, 4333, 4787
Hsp92I GRCGYC 4 cut(s) 1362, 2529, 4228, 4333
HspAI GCGC 4 cut(s) 638, 1720, 2950, 5210
KpnI GGTACC 1 cut(s) 494
Ksp22I TGATCA 2 cut(s) 2283, 2754
KspAI GTTAAC 1 cut(s) 3513
LguI GCTCTTC 3 cut(s) 1451, 5392, 5498
MabI ACCWGGT 1 cut(s) 999
MaeII ACGT 7 cut(s) 268, 955, 1362, 3515, 3529, 4333, 4787
MaeIII GTNAC 9 cut(s) 1468, 1726, 3008, 3485, 3611, 3916, 4297, 4691, 5122
MfeI CAATTG 1 cut(s) 3666
MflI RGATCY 6 cut(s) 250, 1334, 2302, 4368, 4610, 4923
MhlI GDGCHC 8 cut(s) 11, 480, 649, 1750, 2928, 3394, 5133, 5323
MlsI TGGCCA 4 cut(s) 440, 1120, 2763, 3972
MluNI TGGCCA 4 cut(s) 440, 1120, 2763, 3972
MlyI GAGTC 7 cut(s) 300, 1333, 1603, 1978, 2129, 3028, 4749
Mox20I TGGCCA 4 cut(s) 440, 1120, 2763, 3972
Mph1103I ATGCAT 4 cut(s) 687, 1453, 1782, 4756
MroXI GAANNNNTTC 4 cut(s) 1580, 2603, 3054, 3871
MscI TGGCCA 4 cut(s) 440, 1120, 2763, 3972
MslI CAYNNNNRTG 9 cut(s) 1155, 1388, 1785, 2020, 2921, 2984, 3740, 4469, 4523
Msp20I TGGCCA 4 cut(s) 440, 1120, 2763, 3972
MspA1I CMGCKG 5 cut(s) 15, 3823, 3949, 4531, 4537
MspCI CTTAAG 1 cut(s) 4045
MspI CCGG 5 cut(s) 166, 428, 3864, 5438, 5541
MunI CAATTG 1 cut(s) 3666
Mva1269I GAATGC 9 cut(s) 687, 1150, 1453, 1782, 1951, 2486, 4254, 4756, 5504
MvaI CCWGG 8 cut(s) 442, 1001, 1997, 3155, 3305, 3651, 4373, 4816
MvnI CGCG 1 cut(s) 34
NciI CCSGG 2 cut(s) 167, 5542
NcoI CCATGG 3 cut(s) 223, 246, 4464
NdeI CATATG 1 cut(s) 1396
NheI GCTAGC 3 cut(s) 1843, 2369, 2665
NlaIV GGNNCC 6 cut(s) 19, 252, 492, 1650, 2065, 5459
NmeAIII GCCGAG 1 cut(s) 2447
NmuCI GTSAC 6 cut(s) 1468, 1726, 3008, 3611, 3916, 4691
NotI GCGGCCGC 1 cut(s) 35
NsiI ATGCAT 4 cut(s) 687, 1453, 1782, 4756
NspI RCATGY 3 cut(s) 2989, 3016, 5025
NspV TTCGAA 1 cut(s) 2215
PagI TCATGA 4 cut(s) 1389, 2979, 4105, 5238
PceI AGGCCT 1 cut(s) 5517
PciSI GCTCTTC 3 cut(s) 1451, 5392, 5498
PcsI WCGNNNNNNNCGW 3 cut(s) 143, 274, 315
PctI GAATGC 9 cut(s) 687, 1150, 1453, 1782, 1951, 2486, 4254, 4756, 5504
PdmI GAANNNNTTC 4 cut(s) 1580, 2603, 3054, 3871
PflMI CCANNNNNTGG 2 cut(s) 2935, 5316
PfoI TCCNGGA 3 cut(s) 3153, 3303, 4371
PleI GAGTC 7 cut(s) 300, 1332, 1602, 1978, 2128, 3028, 4748
PpsI GAGTC 7 cut(s) 300, 1332, 1602, 1978, 2128, 3028, 4748
Ppu21I YACGTR 2 cut(s) 956, 3530
PpuMI RGGWCCY 1 cut(s) 3653
PsiI TTATAA 5 cut(s) 986, 1623, 2258, 2738, 4097
Psp124BI GAGCTC 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 3653
Psp6I CCWGG 8 cut(s) 440, 999, 1995, 3153, 3303, 3649, 4371, 4814
PspFI CCCAGC 2 cut(s) 4381, 5392
PspGI CCWGG 8 cut(s) 440, 999, 1995, 3153, 3303, 3649, 4371, 4814
PspN4I GGNNCC 6 cut(s) 19, 252, 492, 1650, 2065, 5459
PspPI GGNCC 6 cut(s) 18, 1182, 2109, 3157, 3653, 4456
PspPPI RGGWCCY 1 cut(s) 3653
PstI CTGCAG 2 cut(s) 3123, 4536
PstNI CAGNNNCTG 1 cut(s) 1508
PsuI RGATCY 6 cut(s) 250, 1334, 2302, 4368, 4610, 4923
PvuII CAGCTG 2 cut(s) 4531, 4537
RsaI GTAC 9 cut(s) 465, 492, 954, 1010, 2465, 3185, 3272, 3957, 4682
RsaNI GTAC 9 cut(s) 464, 491, 953, 1009, 2464, 3184, 3271, 3956, 4681
RseI CAYNNNNRTG 9 cut(s) 1155, 1388, 1785, 2020, 2921, 2984, 3740, 4469, 4523
SacI GAGCTC 1 cut(s) 11
SalI GTCGAC 1 cut(s) 715
SapI GCTCTTC 3 cut(s) 1451, 5392, 5498
Sau96I GGNCC 6 cut(s) 18, 1182, 2109, 3157, 3653, 4456
SchI GAGTC 7 cut(s) 300, 1333, 1603, 1978, 2129, 3028, 4749
SduI GDGCHC 8 cut(s) 11, 480, 649, 1750, 2928, 3394, 5133, 5323
SexAI ACCWGGT 1 cut(s) 999
SfuI TTCGAA 1 cut(s) 2215
SinI GGWCC 6 cut(s) 18, 1182, 2109, 3157, 3653, 4456
SmiMI CAYNNNNRTG 9 cut(s) 1155, 1388, 1785, 2020, 2921, 2984, 3740, 4469, 4523
SmlI CTYRAG 4 cut(s) 479, 3500, 4045, 4976
SmoI CTYRAG 4 cut(s) 479, 3500, 4045, 4976
SseBI AGGCCT 1 cut(s) 5517
SspI AATATT 6 cut(s) 1350, 1479, 1960, 2034, 3496, 3837
SstI GAGCTC 1 cut(s) 11
StuI AGGCCT 1 cut(s) 5517
StyI CCWWGG 8 cut(s) 21, 197, 223, 246, 3081, 4398, 4464, 4506
TaiI ACGT 7 cut(s) 271, 958, 1365, 3518, 3532, 4336, 4790
TatI WGTACW 6 cut(s) 463, 1008, 2463, 3183, 3270, 4680
TauI GCSGC 5 cut(s) 37, 40, 219, 1876, 4017
TseFI GTSAC 6 cut(s) 1468, 1726, 3008, 3611, 3916, 4691
Tsp45I GTSAC 6 cut(s) 1468, 1726, 3008, 3611, 3916, 4691
TspGWI ACGGA 9 cut(s) 113, 632, 2307, 2597, 4344, 4602, 4833, 4898, 4982
Van91I CCANNNNNTGG 2 cut(s) 2935, 5316
Vha464I CTTAAG 1 cut(s) 4045
VpaK11BI GGWCC 6 cut(s) 18, 1182, 2109, 3157, 3653, 4456
XbaI TCTAGA 2 cut(s) 3248, 3286
XceI RCATGY 3 cut(s) 2989, 3016, 5025
XcmI CCANNNNNNNNNTGG 1 cut(s) 4801
XmaJI CCTAGG 1 cut(s) 197
XmiI GTMKAC 4 cut(s) 716, 2568, 2578, 5161
XmnI GAANNNNTTC 4 cut(s) 1580, 2603, 3054, 3871
ZraI GACGTC 2 cut(s) 1363, 4334
Zsp2I ATGCAT 4 cut(s) 687, 1453, 1782, 4756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.