AT1G06730

carbohydrate kinase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009506
Physical Location & Seq
Forward (+)
2067509 .. 2070455
2947 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G06730.1

Sequence Viewer

Length: 1467 bp
ATGTATCACCACGCTTTTTCCCTAAAATTTCACTCCTTCTCTCCTCACTATGCGTTATCCCTCTCCAATTTCTCCACCCAAAGCCCTAATCCTCTCCTCCCGCCATTCGCTAGAGTCCCTCATGTGACCAGCTCTGTTTGCCTCCGCTGCCGGAGCAGCGCCGCTGATGTATCTCCGGTTATATACGCCGATGGCTCCTCCTCCATCTGTTCTTTCGGGGAAACTGGAGATGTCGCGGTGGTCGAGAAACCTATAGATGTCTCAACCTTGGGTAATCTCTGCGTCGACATTGTTCTCAGTGTTCACGAATTGCCCCCTCCTTCTCGTGGAGAACGCAAGGCCCTCATGGACGAACTCTCTATGTCTCCCCCAGATAAGAAATACTGGGAAGCAGGTGGCAACTGTAACATGGCTATAGCAGCTGCTAGATTGGGGCTTCATTGTGTTGCTATTGGTCACGTTGGGGATGAAATCTATGGTGAGTTTCTTCTTGATGTGCTTCATGAGGAAGGAATAGGTACGGTTGCATTGAATGGAGGAACAAATGAAAAAGATACCAGCTCATTCTGTGAAACTCTCATTTGCTGGGTTCTTGTGGATCCTTTGCAGAGGCATGGGTTTTGCAGTCGAGCTGATTTCAAGGAGGAACCTGCTTTCAGTTGGATCACTGACCTATCTGATGAAGTAAAGATGGCAATCAGACAGTCAAAAGTTCTTTTCTGCAATGGTTATGACTTTGACGATTTCAGCCCTAGTTTTATTATGTCAACGATAGATTACGCTGCCAAAGTTGGGACAGCTATCTTCTTCGATCCTGGACCACGGGGAAAGAGTCTGTCCAAGGGAACTCCTGATGAGCGTAGAGCACTTGCTCATTTCTTAAGAATGAGTGATGTTCTTCTTCTAACATCTGAGGAGGTGGAGGCTCTAACTGGCATCAGGAACCCTGTTAAAGCAGGACAAGAAATTCTGAGGAACGGAAAGGGAACGAAATGGGTGATTGTAAAGATGGGCCCAAAGGGTTCAATTCTAGTGACGAAATCTAGTGTCTCAGTGGCACCTGCGTTTAAGGTGGAAGTGGTTGACACTGTTGGATGTGGAGACAGTTTTGTGGCTGCAATTGCATTAGGTTATATACGCAACATGCCGCTTGTTAATACCTTAACAATTGCAAATGCGGTTGGAGCAGCAACTGCAATGGGCTGTGGAGCAGGTAGAAATGTTGCAAAAAGGCATCAGGTGGTGGATCTGATGAAGGCCTCGAAACTCAATGATGAAGAGAAGTTTTTCGAACAACTCCTGGCTGAAAATTCAGAGTCATCTAGAATCAATCTTCTTTCAAAAGGGATGATAAAGGATGGGAGGAGCAACAAGCAGCTGATAGAAACAATCTCAATGGAAAAAGTGGTTTCTGAATTGCTTGCAGAACTTGAACTTGGGAGGTGTTGTGTGAAGGCTTCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

488

Amino Acids

52.76

Weight (kDa)

5.89

Isoelectric Point (pI)

38.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 127 - 404 8e-37 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 383, 1069
Acc36I ACCTGC 4 cut(s) 383, 658, 1069, 1202
AccB1I GGYRCC 1 cut(s) 1057
AccI GTMKAC 1 cut(s) 285
AccII CGCG 1 cut(s) 236
AciI CCGC 6 cut(s) 101, 145, 162, 236, 1148, 1178
AclWI GGATC 5 cut(s) 593, 606, 671, 806, 1254
AcsI RAATTY 3 cut(s) 26, 966, 1309
AfaI GTAC 1 cut(s) 520
AfiI CCNNNNNNNGG 2 cut(s) 326, 792
AflII CTTAAG 1 cut(s) 880
AgsI TTSAA 5 cut(s) 532, 640, 1026, 1341, 1433
AjnI CCWGG 2 cut(s) 814, 1299
AjuI GAANNNNNNNTTGG 2 cut(s) 1419, 1451
AluBI AGCT 6 cut(s) 132, 422, 561, 632, 800, 1378
AluI AGCT 6 cut(s) 132, 422, 561, 632, 800, 1378
Alw21I GWGCWC 1 cut(s) 868
Alw26I GTCTC 4 cut(s) 265, 369, 1054, 1095
AlwI GGATC 5 cut(s) 593, 606, 671, 806, 1254
AlwNI CAGNNNCTG 1 cut(s) 1193
AoxI GGCC 3 cut(s) 339, 1012, 1257
ApaI GGGCCC 1 cut(s) 1016
ApeKI GCWGC 8 cut(s) 147, 156, 419, 422, 782, 1115, 1187, 1375
ApoI RAATTY 3 cut(s) 26, 966, 1309
Asp700I GAANNNNTTC 1 cut(s) 1286
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 4 cut(s) 340, 818, 1012, 1013
AsuHPI GGTGA 2 cut(s) 491, 1009
AsuII TTCGAA 1 cut(s) 1290
AvaII GGWCC 1 cut(s) 818
BaeGI GKGCMC 1 cut(s) 1016
BamHI GGATCC 1 cut(s) 598
BanI GGYRCC 1 cut(s) 1057
BanII GRGCYC 1 cut(s) 1016
BauI CACGAG 1 cut(s) 324
Bbv12I GWGCWC 1 cut(s) 868
BbvI GCAGC 8 cut(s) 134, 168, 409, 431, 769, 1102, 1199, 1387
BccI CCATC 5 cut(s) 185, 212, 685, 1003, 1352
BciT130I CCWGG 2 cut(s) 816, 1301
BcoDI GTCTC 4 cut(s) 265, 369, 1054, 1095
BfaI CTAG 6 cut(s) 111, 426, 753, 1031, 1044, 1323
BfmI CTRYAG 2 cut(s) 252, 414
BfoI RGCGCY 1 cut(s) 162
BfrI CTTAAG 1 cut(s) 880
BfuAI ACCTGC 4 cut(s) 383, 658, 1069, 1202
Bme1390I CCNGG 2 cut(s) 816, 1301
Bme18I GGWCC 1 cut(s) 818
BmgT120I GGNCC 4 cut(s) 340, 818, 1012, 1013
BmiI GGNNCC 6 cut(s) 196, 600, 648, 944, 1014, 1059
BmrFI CCNGG 2 cut(s) 816, 1301
BmrI ACTGGG 1 cut(s) 394
BmsI GCATC 2 cut(s) 945, 1243
BmuI ACTGGG 1 cut(s) 394
BpmI CTGGAG 1 cut(s) 246
Bpu14I TTCGAA 1 cut(s) 1290
BsaBI GATNNNNATC 1 cut(s) 695
BsaJI CCNNGG 3 cut(s) 267, 821, 840
BsaWI WCCGGW 1 cut(s) 175
BsaXI ACNNNNNCTCC 2 cut(s) 1432, 1462
Bsc4I CCNNNNNNNGG 2 cut(s) 326, 792
Bse1I ACTGG 3 cut(s) 229, 389, 937
Bse3DI GCAATG 2 cut(s) 730, 1203
Bse8I GATNNNNATC 1 cut(s) 695
BseBI CCWGG 2 cut(s) 816, 1301
BseDI CCNNGG 3 cut(s) 267, 821, 840
BseGI GGATG 4 cut(s) 472, 1100, 1353, 1363
BseJI GATNNNNATC 1 cut(s) 695
BseLI CCNNNNNNNGG 2 cut(s) 326, 792
BseMI GCAATG 2 cut(s) 730, 1203
BseMII CTCAG 4 cut(s) 310, 903, 962, 1065
BseNI ACTGG 3 cut(s) 229, 389, 937
BseRI GAGGAG 6 cut(s) 33, 86, 187, 190, 929, 1378
BseSI GKGCMC 1 cut(s) 1016
BseXI GCAGC 8 cut(s) 134, 168, 409, 431, 769, 1102, 1199, 1387
BseYI CCCAGC 1 cut(s) 585
Bsh1236I CGCG 1 cut(s) 236
BshFI GGCC 3 cut(s) 341, 1014, 1259
BshNI GGYRCC 1 cut(s) 1057
BsiHKAI GWGCWC 1 cut(s) 868
BsiSI CCGG 2 cut(s) 151, 176
BslFI GGGAC 2 cut(s) 101, 808
BslI CCNNNNNNNGG 2 cut(s) 326, 792
BsmAI GTCTC 4 cut(s) 265, 369, 1054, 1095
BsmFI GGGAC 2 cut(s) 101, 808
BsnI GGCC 3 cut(s) 341, 1014, 1259
Bsp119I TTCGAA 1 cut(s) 1290
Bsp120I GGGCCC 1 cut(s) 1012
Bsp1286I GDGCHC 2 cut(s) 868, 1016
Bsp143I GATC 4 cut(s) 598, 663, 811, 1246
BspACI CCGC 6 cut(s) 101, 145, 162, 236, 1148, 1178
BspANI GGCC 3 cut(s) 341, 1014, 1259
BspCNI CTCAG 4 cut(s) 309, 904, 963, 1064
BspFNI CGCG 1 cut(s) 236
BspHI TCATGA 1 cut(s) 502
BspLI GGNNCC 6 cut(s) 196, 600, 648, 944, 1014, 1059
BspMI ACCTGC 4 cut(s) 383, 658, 1069, 1202
BspPI GGATC 5 cut(s) 593, 606, 671, 806, 1254
BspT104I TTCGAA 1 cut(s) 1290
BspT107I GGYRCC 1 cut(s) 1057
BspTI CTTAAG 1 cut(s) 880
BsrDI GCAATG 2 cut(s) 730, 1203
BsrI ACTGG 3 cut(s) 229, 389, 937
BssECI CCNNGG 3 cut(s) 267, 821, 840
BssMI GATC 4 cut(s) 598, 663, 811, 1246
BssSI CACGAG 1 cut(s) 324
BssT1I CCWWGG 2 cut(s) 267, 840
Bst2BI CACGAG 1 cut(s) 324
Bst2UI CCWGG 2 cut(s) 816, 1301
Bst4CI ACNGT 5 cut(s) 404, 523, 705, 1090, 1106
Bst6I CTCTTC 1 cut(s) 1272
BstAFI CTTAAG 1 cut(s) 880
BstAPI GCANNNNNTGC 1 cut(s) 1193
BstBI TTCGAA 1 cut(s) 1290
BstC8I GCNNGC 1 cut(s) 1422
BstDEI CTNAG 4 cut(s) 296, 912, 971, 1051
BstDSI CCRYGG 1 cut(s) 821
BstF5I GGATG 4 cut(s) 472, 1100, 1353, 1363
BstFNI CGCG 1 cut(s) 236
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 4 cut(s) 601, 666, 814, 1249
BstMAI GTCTC 4 cut(s) 265, 369, 1054, 1095
BstMBI GATC 4 cut(s) 598, 663, 811, 1246
BstMWI GCNNNNNNNGC 8 cut(s) 138, 147, 153, 156, 419, 1121, 1184, 1193
BstNI CCWGG 2 cut(s) 816, 1301
BstNSI RCATGY 1 cut(s) 1147
BstSCI CCNGG 2 cut(s) 814, 1299
BstSFI CTRYAG 2 cut(s) 252, 414
BstSLI GKGCMC 1 cut(s) 1016
BstUI CGCG 1 cut(s) 236
BstV1I GCAGC 8 cut(s) 134, 168, 409, 431, 769, 1102, 1199, 1387
BstX2I RGATCY 2 cut(s) 598, 1246
BstYI RGATCY 2 cut(s) 598, 1246
BsuRI GGCC 3 cut(s) 341, 1014, 1259
BtgI CCRYGG 1 cut(s) 821
BtsCI GGATG 4 cut(s) 472, 1100, 1353, 1363
BtsIMutI CAGTG 4 cut(s) 304, 666, 1059, 1086
BveI ACCTGC 4 cut(s) 383, 658, 1069, 1202
Cac8I GCNNGC 1 cut(s) 1422
CaiI CAGNNNCTG 1 cut(s) 1193
CciI TCATGA 1 cut(s) 502
CfoI GCGC 1 cut(s) 161
Cfr13I GGNCC 4 cut(s) 340, 818, 1012, 1013
CseI GACGC 1 cut(s) 271
Csp6I GTAC 1 cut(s) 519
CviAII CATG 6 cut(s) 122, 346, 409, 503, 614, 1144
CviQI GTAC 1 cut(s) 519
DdeI CTNAG 4 cut(s) 296, 912, 971, 1051
DpnI GATC 4 cut(s) 600, 665, 813, 1248
DpnII GATC 4 cut(s) 598, 663, 811, 1246
Eam1104I CTCTTC 1 cut(s) 1272
EarI CTCTTC 1 cut(s) 1272
Eco130I CCWWGG 2 cut(s) 267, 840
Eco147I AGGCCT 1 cut(s) 1259
Eco24I GRGCYC 1 cut(s) 1016
Eco47I GGWCC 1 cut(s) 818
EcoO109I RGGNCCY 1 cut(s) 340
EcoRII CCWGG 2 cut(s) 814, 1299
EcoT14I CCWWGG 2 cut(s) 267, 840
EcoT38I GRGCYC 1 cut(s) 1016
ErhI CCWWGG 2 cut(s) 267, 840
FaeI CATG 6 cut(s) 125, 349, 412, 506, 617, 1147
FaqI GGGAC 2 cut(s) 101, 808
FatI CATG 6 cut(s) 121, 345, 408, 502, 613, 1143
FauI CCCGC 1 cut(s) 108
FblI GTMKAC 1 cut(s) 285
FokI GGATG 4 cut(s) 479, 1107, 1360, 1370
FriOI GRGCYC 1 cut(s) 1016
FspBI CTAG 6 cut(s) 111, 426, 753, 1031, 1044, 1323
GlaI GCGC 1 cut(s) 160
GsaI CCCAGC 1 cut(s) 589
GsuI CTGGAG 1 cut(s) 246
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 3 cut(s) 341, 1014, 1259
HapII CCGG 2 cut(s) 151, 176
HgaI GACGC 1 cut(s) 271
HhaI GCGC 1 cut(s) 161
Hin1II CATG 6 cut(s) 125, 349, 412, 506, 617, 1147
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HincII GTYRAC 3 cut(s) 286, 768, 1084
HindII GTYRAC 3 cut(s) 286, 768, 1084
HinfI GANTC 4 cut(s) 114, 832, 1316, 1326
HpaII CCGG 2 cut(s) 151, 176
HphI GGTGA 2 cut(s) 491, 1009
Hpy166II GTNNAC 4 cut(s) 286, 304, 768, 1084
Hpy188I TCNGA 7 cut(s) 679, 701, 913, 972, 1251, 1315, 1414
Hpy188III TCNNGA 8 cut(s) 244, 305, 491, 503, 851, 940, 1323, 1464
Hpy8I GTNNAC 4 cut(s) 286, 304, 768, 1084
Hpy99I CGWCG 1 cut(s) 287
HpyAV CCTTC 5 cut(s) 46, 330, 503, 1249, 1447
HpyCH4III ACNGT 5 cut(s) 404, 523, 705, 1090, 1106
HpyCH4IV ACGT 1 cut(s) 459
HpyF10VI GCNNNNNNNGC 8 cut(s) 138, 147, 153, 156, 419, 1121, 1184, 1193
HpyF3I CTNAG 4 cut(s) 296, 912, 971, 1051
HpySE526I ACGT 1 cut(s) 459
Hsp92II CATG 6 cut(s) 125, 349, 412, 506, 617, 1147
HspAI GCGC 1 cut(s) 159
Kzo9I GATC 4 cut(s) 598, 663, 811, 1246
LmnI GCTCC 5 cut(s) 153, 200, 1184, 1208, 1365
Lsp1109I GCAGC 8 cut(s) 134, 168, 409, 431, 769, 1102, 1199, 1387
LweI GCATC 2 cut(s) 945, 1243
MaeI CTAG 6 cut(s) 111, 426, 753, 1031, 1044, 1323
MaeII ACGT 1 cut(s) 459
MaeIII GTNAC 4 cut(s) 124, 404, 455, 1033
MalI GATC 4 cut(s) 600, 665, 813, 1248
MboI GATC 4 cut(s) 598, 663, 811, 1246
MboII GAAGA 7 cut(s) 479, 796, 799, 890, 893, 1289, 1325
MfeI CAATTG 2 cut(s) 1119, 1167
MflI RGATCY 2 cut(s) 598, 1246
MhlI GDGCHC 2 cut(s) 868, 1016
MluCI AATT 9 cut(s) 26, 67, 308, 966, 1026, 1119, 1167, 1309, 1415
MlyI GAGTC 3 cut(s) 123, 841, 1325
MmeI TCCRAC 3 cut(s) 641, 1072, 1162
MroXI GAANNNNTTC 1 cut(s) 1286
MseI TTAA 5 cut(s) 881, 951, 1068, 1155, 1163
MspA1I CMGCKG 4 cut(s) 147, 164, 422, 1378
MspCI CTTAAG 1 cut(s) 880
MspI CCGG 2 cut(s) 151, 176
MspR9I CCNGG 2 cut(s) 816, 1301
MunI CAATTG 2 cut(s) 1119, 1167
MvaI CCWGG 2 cut(s) 816, 1301
MvnI CGCG 1 cut(s) 236
MwoI GCNNNNNNNGC 8 cut(s) 138, 147, 153, 156, 419, 1121, 1184, 1193
NdeII GATC 4 cut(s) 598, 663, 811, 1246
NlaIII CATG 6 cut(s) 125, 349, 412, 506, 617, 1147
NlaIV GGNNCC 6 cut(s) 196, 600, 648, 944, 1014, 1059
NmuCI GTSAC 3 cut(s) 124, 455, 1033
NspI RCATGY 1 cut(s) 1147
NspV TTCGAA 1 cut(s) 1290
PagI TCATGA 1 cut(s) 502
PaqCI CACCTGC 2 cut(s) 383, 1069
PceI AGGCCT 1 cut(s) 1259
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PdmI GAANNNNTTC 1 cut(s) 1286
PfeI GAWTC 1 cut(s) 1326
PfoI TCCNGGA 1 cut(s) 814
PleI GAGTC 3 cut(s) 122, 840, 1324
PpsI GAGTC 3 cut(s) 122, 840, 1324
Psp6I CCWGG 2 cut(s) 814, 1299
PspFI CCCAGC 1 cut(s) 585
PspGI CCWGG 2 cut(s) 814, 1299
PspN4I GGNNCC 6 cut(s) 196, 600, 648, 944, 1014, 1059
PspOMI GGGCCC 1 cut(s) 1012
PspPI GGNCC 4 cut(s) 340, 818, 1012, 1013
PstNI CAGNNNCTG 1 cut(s) 1193
PsuI RGATCY 2 cut(s) 598, 1246
PvuII CAGCTG 2 cut(s) 422, 1378
RsaI GTAC 1 cut(s) 520
RsaNI GTAC 1 cut(s) 519
SalI GTCGAC 1 cut(s) 284
SaqAI TTAA 5 cut(s) 881, 951, 1068, 1155, 1163
Sau3AI GATC 4 cut(s) 598, 663, 811, 1246
Sau96I GGNCC 4 cut(s) 340, 818, 1012, 1013
SchI GAGTC 3 cut(s) 123, 841, 1325
ScrFI CCNGG 2 cut(s) 816, 1301
SduI GDGCHC 2 cut(s) 868, 1016
SfaNI GCATC 2 cut(s) 945, 1243
SfcI CTRYAG 2 cut(s) 252, 414
SfuI TTCGAA 1 cut(s) 1290
SinI GGWCC 1 cut(s) 818
SmlI CTYRAG 1 cut(s) 880
SmoI CTYRAG 1 cut(s) 880
Sse9I AATT 9 cut(s) 26, 67, 308, 966, 1026, 1119, 1167, 1309, 1415
SseBI AGGCCT 1 cut(s) 1259
SsiI CCGC 6 cut(s) 101, 145, 162, 236, 1148, 1178
SspMI CTAG 6 cut(s) 111, 426, 753, 1031, 1044, 1323
StuI AGGCCT 1 cut(s) 1259
StyD4I CCNGG 2 cut(s) 814, 1299
StyI CCWWGG 2 cut(s) 267, 840
TaaI ACNGT 5 cut(s) 404, 523, 705, 1090, 1106
TaiI ACGT 1 cut(s) 462
TaqI TCGA 6 cut(s) 243, 285, 628, 810, 1262, 1290
TasI AATT 9 cut(s) 26, 67, 308, 966, 1026, 1119, 1167, 1309, 1415
TauI GCSGC 2 cut(s) 164, 1150
TfiI GAWTC 1 cut(s) 1326
Tru1I TTAA 5 cut(s) 881, 951, 1068, 1155, 1163
Tru9I TTAA 5 cut(s) 881, 951, 1068, 1155, 1163
TscAI CASTG 4 cut(s) 304, 673, 1059, 1093
TseFI GTSAC 3 cut(s) 124, 455, 1033
TseI GCWGC 8 cut(s) 147, 156, 419, 422, 782, 1115, 1187, 1375
Tsp45I GTSAC 3 cut(s) 124, 455, 1033
TspDTI ATGAA 8 cut(s) 428, 483, 491, 561, 696, 1268, 1290, 1449
TspGWI ACGGA 1 cut(s) 993
TspRI CASTG 4 cut(s) 304, 673, 1059, 1093
Vha464I CTTAAG 1 cut(s) 880
VpaK11BI GGWCC 1 cut(s) 818
XapI RAATTY 3 cut(s) 26, 966, 1309
XbaI TCTAGA 1 cut(s) 1322
XceI RCATGY 1 cut(s) 1147
XmiI GTMKAC 1 cut(s) 285
XmnI GAANNNNTTC 1 cut(s) 1286
XspI CTAG 6 cut(s) 111, 426, 753, 1031, 1044, 1323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.