Rorug06G0201300

pfkB family carbohydrate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0009506
Physical Location & Seq
Reverse (-)
32315521 .. 32320116
4596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0201300.1

Sequence Viewer

Length: 1071 bp
ATGGTGGGTGCGTCACTAGGAATGGTGATCCGGGTGGATCAGAATGCTTTGAAAGGGAAAGAAGAAGAACGAAGACTCAAAATAGTGTTTGATGTTCGCTGCCGGATTCGTGTCTGGAAGGTTTTTGAGTTTTCTCCAATTGTGAGACCGGAGTTAACTTTCATCTATGAGAAAGTTAAGAGATTTTGTCGTGAATGTGGACTGTTCTTCCATGATGCCTTAGGTTGTGATAAGTTATTGATCGAAGAGAAGGAGGAGGTTCAGGCGAAGCCACCGGCGATGGCCATGGCAGGATTATCTTTTACCTCGGGGATAGAGGCTGGTCCTTCTGTGACAGACAAAGGTTTGGGGATGGGTAACACCGATTTGGTTCGCATGGAACTTGAATCGGGTTCCTCTGGGATTGGTGCAGAGGCTTTGCTCGTTGGAAAGGGAGGCCATGCAACGTTGACAACTAATGGTTTCATGAACTCTAGGATGATGGGAAACCCTATGTTGTTGGATCCGGTTTTGAATGATAACCCACCTGTGGGAAGTTTGAAAACTAGTTTTCCACTTTCAGTCATTCAGAAGGAGGCAGGCTCAGAGTCACTGGCTTTGTTCCCAATGTCGACTGTGATAGGGATTCGATGTGTTTTGGATTTGTTTGGAAAGGCTGTGACGTTTCCTGGTGTTTGGCGGAATTTGACTGATGAGGTCGAGGGAGTTTTAGATCATCAGCTCGAGTTAGAGGTCAAGTCGAAGACTGGTATGGGTTTCAATTCAACTTGGAAACGGAGATGTCCTCTTGTACTCCGCATAAAAGGGAGGTCTCTGTCATCTGATTCAGCGTCTGTTCTCCTAAGCGAAGGATCTGAGGCTTCTCACCTGTGTAGAGAAAGAAATTGGATTCGAAGCTTGTTCCGGTTGTTGATCAATTTTAATTCGCATGCAAGAGCAAAAGCTGGGGTGACTCTTCAATCTGGTTTTCACCATTTCTCCACCGTCAATTTTCGGAAAGGTAGGCTTATGACAGTGACCTTGATGCATGCAGTTGTGTTTATCGCTGCCACCACGTCCTGCTTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

356

Amino Acids

39.19

Weight (kDa)

9.01

Isoelectric Point (pI)

34.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 611
AciI CCGC 2 cut(s) 679, 796
AclI AACGTT 1 cut(s) 446
AclWI GGATC 5 cut(s) 22, 45, 497, 510, 859
AcoI YGGCCR 1 cut(s) 282
AcsI RAATTY 1 cut(s) 682
AfaI GTAC 1 cut(s) 792
AfiI CCNNNNNNNGG 2 cut(s) 529, 530
AgsI TTSAA 7 cut(s) 52, 386, 514, 541, 760, 765, 959
AhlI ACTAGT 1 cut(s) 545
AjiI CACGTC 1 cut(s) 1056
AjnI CCWGG 1 cut(s) 667
AluBI AGCT 3 cut(s) 721, 897, 944
AluI AGCT 3 cut(s) 721, 897, 944
Alw26I GTCTC 2 cut(s) 139, 816
AlwI GGATC 5 cut(s) 22, 45, 497, 510, 859
AlwNI CAGNNNCTG 1 cut(s) 833
Ama87I CYCGRG 2 cut(s) 307, 722
AoxI GGCC 2 cut(s) 282, 436
ApeKI GCWGC 2 cut(s) 99, 1046
ApoI RAATTY 1 cut(s) 682
AspS9I GGNCC 1 cut(s) 323
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 4 cut(s) 37, 857, 961, 962
AsuII TTCGAA 1 cut(s) 892
AvaI CYCGRG 2 cut(s) 307, 722
AvaII GGWCC 1 cut(s) 323
AxyI CCTNAGG 1 cut(s) 220
BalI TGGCCA 1 cut(s) 284
BamHI GGATCC 1 cut(s) 502
BbsI GAAGAC 2 cut(s) 79, 749
BbvI GCAGC 2 cut(s) 86, 1033
BccI CCATC 3 cut(s) 274, 346, 475
BciT130I CCWGG 1 cut(s) 669
BclI TGATCA 1 cut(s) 912
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 2 cut(s) 139, 816
BcuI ACTAGT 1 cut(s) 545
BfaI CTAG 3 cut(s) 17, 474, 546
BisI GCNGC 2 cut(s) 100, 1047
BlsI GCNGC 2 cut(s) 101, 1048
Bme1390I CCNGG 2 cut(s) 32, 669
Bme18I GGWCC 1 cut(s) 323
BmeT110I CYCGRG 2 cut(s) 307, 722
BmgBI CACGTC 1 cut(s) 1056
BmgT120I GGNCC 1 cut(s) 323
BmiI GGNNCC 2 cut(s) 394, 504
BmrFI CCNGG 2 cut(s) 32, 669
BmsI GCATC 2 cut(s) 205, 1014
BpiI GAAGAC 2 cut(s) 79, 749
BplI GAGNNNNNCTC 4 cut(s) 566, 598, 769, 801
Bpu10I CCTNAGC 1 cut(s) 842
Bpu14I TTCGAA 1 cut(s) 892
BpuMI CCSGG 1 cut(s) 32
BsaI GGTCTC 2 cut(s) 139, 816
BsaJI CCNNGG 2 cut(s) 285, 306
BsaWI WCCGGW 3 cut(s) 148, 505, 903
BsaXI ACNNNNNCTCC 2 cut(s) 962, 992
Bsc4I CCNNNNNNNGG 2 cut(s) 529, 530
Bse118I RCCGGY 1 cut(s) 274
Bse1I ACTGG 2 cut(s) 597, 751
Bse21I CCTNAGG 1 cut(s) 220
BseBI CCWGG 1 cut(s) 669
BseDI CCNNGG 2 cut(s) 285, 306
BseGI GGATG 2 cut(s) 357, 483
BseLI CCNNNNNNNGG 2 cut(s) 529, 530
BseMII CTCAG 2 cut(s) 597, 846
BseNI ACTGG 2 cut(s) 597, 751
BseRI GAGGAG 1 cut(s) 269
BseXI GCAGC 2 cut(s) 86, 1033
BseYI CCCAGC 1 cut(s) 944
BsgI GTGCAG 1 cut(s) 429
BshFI GGCC 2 cut(s) 284, 438
BsiHKCI CYCGRG 2 cut(s) 307, 722
BsiSI CCGG 6 cut(s) 31, 103, 149, 275, 506, 904
BslI CCNNNNNNNGG 2 cut(s) 529, 530
BsmAI GTCTC 2 cut(s) 139, 816
BsmI GAATGC 1 cut(s) 49
BsnI GGCC 2 cut(s) 284, 438
Bso31I GGTCTC 2 cut(s) 139, 816
BsoBI CYCGRG 2 cut(s) 307, 722
Bsp119I TTCGAA 1 cut(s) 892
Bsp143I GATC 7 cut(s) 27, 37, 240, 502, 712, 851, 912
Bsp19I CCATGG 1 cut(s) 285
BspACI CCGC 2 cut(s) 679, 796
BspANI GGCC 2 cut(s) 284, 438
BspCNI CTCAG 2 cut(s) 596, 847
BspHI TCATGA 1 cut(s) 465
BspLI GGNNCC 2 cut(s) 394, 504
BspPI GGATC 5 cut(s) 22, 45, 497, 510, 859
BspT104I TTCGAA 1 cut(s) 892
BspTNI GGTCTC 2 cut(s) 139, 816
BsrFI RCCGGY 1 cut(s) 274
BsrI ACTGG 2 cut(s) 597, 751
BssAI RCCGGY 1 cut(s) 274
BssECI CCNNGG 2 cut(s) 285, 306
BssMI GATC 7 cut(s) 27, 37, 240, 502, 712, 851, 912
BssT1I CCWWGG 1 cut(s) 285
Bst2UI CCWGG 1 cut(s) 669
Bst4CI ACNGT 4 cut(s) 204, 616, 985, 1015
Bst6I CTCTTC 2 cut(s) 240, 960
BstBI TTCGAA 1 cut(s) 892
BstC8I GCNNGC 3 cut(s) 580, 930, 1029
BstDEI CTNAG 4 cut(s) 220, 583, 842, 855
BstDSI CCRYGG 1 cut(s) 285
BstF5I GGATG 2 cut(s) 357, 483
BstKTI GATC 7 cut(s) 30, 40, 243, 505, 715, 854, 915
BstMAI GTCTC 2 cut(s) 139, 816
BstMBI GATC 7 cut(s) 27, 37, 240, 502, 712, 851, 912
BstNI CCWGG 1 cut(s) 669
BstNSI RCATGY 2 cut(s) 932, 1031
BstSCI CCNGG 2 cut(s) 30, 667
BstV1I GCAGC 2 cut(s) 86, 1033
BstV2I GAAGAC 2 cut(s) 79, 749
BstX2I RGATCY 2 cut(s) 502, 851
BstYI RGATCY 2 cut(s) 502, 851
Bsu36I CCTNAGG 1 cut(s) 220
BsuRI GGCC 2 cut(s) 284, 438
BtgI CCRYGG 1 cut(s) 285
BtgZI GCGATG 1 cut(s) 293
BtrI CACGTC 1 cut(s) 1056
BtsCI GGATG 2 cut(s) 357, 483
BtsIMutI CAGTG 2 cut(s) 590, 1020
Cac8I GCNNGC 3 cut(s) 580, 930, 1029
CaiI CAGNNNCTG 1 cut(s) 833
CciI TCATGA 1 cut(s) 465
Cfr10I RCCGGY 1 cut(s) 274
Cfr13I GGNCC 1 cut(s) 323
CseI GACGC 1 cut(s) 819
Csp6I GTAC 1 cut(s) 791
CviAII CATG 7 cut(s) 212, 286, 376, 440, 466, 929, 1028
CviQI GTAC 1 cut(s) 791
DdeI CTNAG 4 cut(s) 220, 583, 842, 855
DpnI GATC 7 cut(s) 29, 39, 242, 504, 714, 853, 914
DpnII GATC 7 cut(s) 27, 37, 240, 502, 712, 851, 912
EaeI YGGCCR 1 cut(s) 282
Eam1104I CTCTTC 2 cut(s) 240, 960
EarI CTCTTC 2 cut(s) 240, 960
EciI GGCGGA 1 cut(s) 694
Eco130I CCWWGG 1 cut(s) 285
Eco31I GGTCTC 2 cut(s) 139, 816
Eco47I GGWCC 1 cut(s) 323
Eco81I CCTNAGG 1 cut(s) 220
Eco88I CYCGRG 2 cut(s) 307, 722
EcoRII CCWGG 1 cut(s) 667
EcoT14I CCWWGG 1 cut(s) 285
EcoT22I ATGCAT 1 cut(s) 1029
ErhI CCWWGG 1 cut(s) 285
FaeI CATG 7 cut(s) 215, 289, 379, 443, 469, 932, 1031
FalI AAGNNNNNCTT 2 cut(s) 990, 1022
FatI CATG 7 cut(s) 211, 285, 375, 439, 465, 928, 1027
FbaI TGATCA 1 cut(s) 912
FblI GTMKAC 1 cut(s) 611
Fnu4HI GCNGC 2 cut(s) 100, 1047
FokI GGATG 2 cut(s) 364, 490
Fsp4HI GCNGC 2 cut(s) 100, 1047
FspBI CTAG 3 cut(s) 17, 474, 546
GluI GCNGC 2 cut(s) 100, 1047
GsaI CCCAGC 1 cut(s) 948
HaeIII GGCC 2 cut(s) 284, 438
HapII CCGG 6 cut(s) 31, 103, 149, 275, 506, 904
HgaI GACGC 1 cut(s) 819
Hin1II CATG 7 cut(s) 215, 289, 379, 443, 469, 932, 1031
HincII GTYRAC 3 cut(s) 156, 450, 612
HindII GTYRAC 3 cut(s) 156, 450, 612
HindIII AAGCTT 1 cut(s) 895
HinfI GANTC 8 cut(s) 75, 106, 386, 587, 625, 824, 889, 952
HpaI GTTAAC 1 cut(s) 156
HpaII CCGG 6 cut(s) 31, 103, 149, 275, 506, 904
HphI GGTGA 4 cut(s) 37, 857, 961, 962
Hpy166II GTNNAC 4 cut(s) 156, 200, 450, 612
Hpy188I TCNGA 6 cut(s) 42, 570, 586, 823, 856, 996
Hpy188III TCNNGA 3 cut(s) 115, 191, 466
Hpy8I GTNNAC 4 cut(s) 156, 200, 450, 612
HpyAV CCTTC 5 cut(s) 112, 244, 336, 565, 842
HpyCH4III ACNGT 4 cut(s) 204, 616, 985, 1015
HpyCH4IV ACGT 3 cut(s) 446, 662, 1055
HpyCH4V TGCA 5 cut(s) 410, 443, 932, 1027, 1031
HpyF3I CTNAG 4 cut(s) 220, 583, 842, 855
HpySE526I ACGT 3 cut(s) 446, 662, 1055
Hsp92II CATG 7 cut(s) 215, 289, 379, 443, 469, 932, 1031
Ksp22I TGATCA 1 cut(s) 912
KspAI GTTAAC 1 cut(s) 156
Kzo9I GATC 7 cut(s) 27, 37, 240, 502, 712, 851, 912
Lsp1109I GCAGC 2 cut(s) 86, 1033
LweI GCATC 2 cut(s) 205, 1014
MaeI CTAG 3 cut(s) 17, 474, 546
MaeII ACGT 3 cut(s) 446, 662, 1055
MaeIII GTNAC 7 cut(s) 12, 331, 356, 588, 658, 949, 1015
MalI GATC 7 cut(s) 29, 39, 242, 504, 714, 853, 914
MboI GATC 7 cut(s) 27, 37, 240, 502, 712, 851, 912
MboII GAAGA 7 cut(s) 74, 77, 84, 199, 257, 754, 947
MfeI CAATTG 1 cut(s) 138
MflI RGATCY 2 cut(s) 502, 851
MlsI TGGCCA 1 cut(s) 284
MluCI AATT 7 cut(s) 138, 682, 760, 883, 916, 922, 988
MluNI TGGCCA 1 cut(s) 284
MlyI GAGTC 3 cut(s) 69, 596, 946
MmeI TCCRAC 2 cut(s) 406, 480
Mox20I TGGCCA 1 cut(s) 284
Mph1103I ATGCAT 1 cut(s) 1029
MscI TGGCCA 1 cut(s) 284
MseI TTAA 4 cut(s) 155, 177, 921, 1069
Msp20I TGGCCA 1 cut(s) 284
MspI CCGG 6 cut(s) 31, 103, 149, 275, 506, 904
MspR9I CCNGG 2 cut(s) 32, 669
MunI CAATTG 1 cut(s) 138
Mva1269I GAATGC 1 cut(s) 49
MvaI CCWGG 1 cut(s) 669
NciI CCSGG 1 cut(s) 32
NcoI CCATGG 1 cut(s) 285
NdeII GATC 7 cut(s) 27, 37, 240, 502, 712, 851, 912
NlaIII CATG 7 cut(s) 215, 289, 379, 443, 469, 932, 1031
NlaIV GGNNCC 2 cut(s) 394, 504
NmuCI GTSAC 6 cut(s) 12, 331, 588, 658, 949, 1015
NsiI ATGCAT 1 cut(s) 1029
NspI RCATGY 2 cut(s) 932, 1031
NspV TTCGAA 1 cut(s) 892
PaeI GCATGC 2 cut(s) 932, 1031
PaeR7I CTCGAG 1 cut(s) 722
PagI TCATGA 1 cut(s) 465
PctI GAATGC 1 cut(s) 49
PfeI GAWTC 5 cut(s) 106, 386, 625, 824, 889
PkrI GCNGC 2 cut(s) 101, 1048
PleI GAGTC 3 cut(s) 69, 595, 946
PpsI GAGTC 3 cut(s) 69, 595, 946
Psp1406I AACGTT 1 cut(s) 446
Psp6I CCWGG 1 cut(s) 667
PspFI CCCAGC 1 cut(s) 944
PspGI CCWGG 1 cut(s) 667
PspN4I GGNNCC 2 cut(s) 394, 504
PspPI GGNCC 1 cut(s) 323
PspXI VCTCGAGB 1 cut(s) 722
PstNI CAGNNNCTG 1 cut(s) 833
PsuI RGATCY 2 cut(s) 502, 851
RsaI GTAC 1 cut(s) 792
RsaNI GTAC 1 cut(s) 791
SalI GTCGAC 1 cut(s) 610
SaqAI TTAA 4 cut(s) 155, 177, 921, 1069
SatI GCNGC 2 cut(s) 100, 1047
Sau3AI GATC 7 cut(s) 27, 37, 240, 502, 712, 851, 912
Sau96I GGNCC 1 cut(s) 323
SchI GAGTC 3 cut(s) 69, 596, 946
ScrFI CCNGG 2 cut(s) 32, 669
SfaNI GCATC 2 cut(s) 205, 1014
Sfr274I CTCGAG 1 cut(s) 722
SfuI TTCGAA 1 cut(s) 892
SgrAI CRCCGGYG 1 cut(s) 274
SinI GGWCC 1 cut(s) 323
SlaI CTCGAG 1 cut(s) 722
SmlI CTYRAG 1 cut(s) 722
SmoI CTYRAG 1 cut(s) 722
SpeI ACTAGT 1 cut(s) 545
SphI GCATGC 2 cut(s) 932, 1031
Sse9I AATT 7 cut(s) 138, 682, 760, 883, 916, 922, 988
SsiI CCGC 2 cut(s) 679, 796
SspMI CTAG 3 cut(s) 17, 474, 546
StyD4I CCNGG 2 cut(s) 30, 667
StyI CCWWGG 1 cut(s) 285
TaaI ACNGT 4 cut(s) 204, 616, 985, 1015
TaiI ACGT 3 cut(s) 449, 665, 1058
TaqI TCGA 7 cut(s) 243, 611, 628, 699, 723, 740, 892
TasI AATT 7 cut(s) 138, 682, 760, 883, 916, 922, 988
TatI WGTACW 1 cut(s) 790
TfiI GAWTC 5 cut(s) 106, 386, 625, 824, 889
Tru1I TTAA 4 cut(s) 155, 177, 921, 1069
Tru9I TTAA 4 cut(s) 155, 177, 921, 1069
TscAI CASTG 2 cut(s) 597, 1020
TseFI GTSAC 6 cut(s) 12, 331, 588, 658, 949, 1015
TseI GCWGC 2 cut(s) 99, 1046
Tsp45I GTSAC 6 cut(s) 12, 331, 588, 658, 949, 1015
TspDTI ATGAA 3 cut(s) 151, 454, 482
TspGWI ACGGA 1 cut(s) 790
TspRI CASTG 2 cut(s) 597, 1020
VpaK11BI GGWCC 1 cut(s) 323
XapI RAATTY 1 cut(s) 682
XceI RCATGY 2 cut(s) 932, 1031
XhoI CTCGAG 1 cut(s) 722
XmiI GTMKAC 1 cut(s) 611
XspI CTAG 3 cut(s) 17, 474, 546
Zsp2I ATGCAT 1 cut(s) 1029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.