MD10G1190800.v1.1

pfkB family carbohydrate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009506
Physical Location & Seq
Forward (+)
28779110 .. 28782337
3228 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1190800.v1.1.491

Sequence Viewer

Length: 1473 bp
ATGCATCACTGCACCGTAACCCTGAAACCCCCAATTCCACACCATGTATCCTCCTCTCACTCCCACTTGATTTTTCCCCCCAGCCCTGGGCTGACCAAATCCCAATCCCACCCCATCTTCGCGCCAATATACATTCGCGCTCATTCCGTCTCCAACCGCACCGCCAGAGGGCTCGATGTCTCCGTCTCCAAACACTCCCCAAACTCCAGCACCCCAAATGGCTCCGTACACGATTGGAAGCCGAAGAGCGTCGGCGTTAAAGACGTAGATGTCGCCACTCTCGGCAATCTCTGCGTGGATATCGTGCTCAATGTGCCCAAATTGCCACCGCCCGATGTCCACGACCGCAAATCTTACATGGACCGGTTGTCCGCCTCGCCGCCGGATAAGCAATACTGGGAAGCTGGTGGAAACTGCAATATGGCCATAGCAGCTGCAAGATTAGGACTTAAGTGCACCGCAATTGGGCACGTGGGTAACGAAGTATATGGACAGTTCCTTTTAGATGTCCTTCAAGATGAAGGAATTAGCATGGTTGGAATGAGTGACAACAATACCAATGTTGACAGTTCTAGTGCATCGTATGAAACACTTTTATGCTGGGTACTCGTTGATCCATTGCAAAGACATGGTTTTTGCAGCCGAGCTGATTTCAGTAAGGAGCCTGCATTCAGTTGGATGAGCAAATTATCTGGACAAGTAAAGACAGCTATAAAACAGTCAAAGATTCTGTTCTGCAATGGTTATGGCTTTGATGAGCTCTCTCCTGGTGTAATAGCCGCAGCTGTAGAGTATGCCGTTGACGTTGGTACAGCAATTTTCTTCGACCCTGGACCACGTGGAAAAAGCCTTTCTGCTGGTACAACAGAAGAACGAGAAGCACTTAACCTGTTGTTGAGGATGAGTGATGTTCTTCTCCTAACGTCTGATGAGGCTGAGTCATTGACGAGCATTAAAAACCCAATATTAGCAGGGCAGGAGCTGCTCAAGCAAGGGGTGCACACAAAATGGGTGATTGTCAAAATGGGTCCAAGGGGTTCAATTCTAATAACTAGGTCAAGTATTTCTTTTGCACCAGCTTTCGAGGTAAATGTTGTAGATACTGTTGGATGTGGAGATAGTTTCGTGGCTGCTATTGCATATGGTTTTATCCACAACATGCCGATGGTTAACACATTAGCAATTGCAAATGCAGTAGGTGCAGCAACTGCCACAGGTTGTGGTGCTGGTAGGAATGTGGCAACCTTGGAGAAAGTAATAGAACTTATAAGGGCATCTAATCTCAAGGAGGATGACAAATTTTGGAATGAGTTAATTGATGAAAATTTGGGTGTCGAGGAAATTACGTCCCTCTCAAATTTGGTCATCAATGGAAGTGATAACCAGTTAAATTGTGTGTCCTTGCAGAAGGTGGTATCCGAATTGCTTCCTAAGCTCAAATCTGCACAGTTAGAAAGAAAAGTATCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

491

Amino Acids

52.73

Weight (kDa)

6.21

Isoelectric Point (pI)

32.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 131 - 411 6.6e-36 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1268
AasI GACNNNNNNGTC 1 cut(s) 269
AccII CGCG 2 cut(s) 122, 138
AciI CCGC 8 cut(s) 157, 162, 329, 346, 372, 380, 459, 780
AclWI GGATC 1 cut(s) 608
AcoI YGGCCR 1 cut(s) 423
AcsI RAATTY 3 cut(s) 1298, 1324, 1357
AcvI CACGTG 2 cut(s) 472, 839
AfaI GTAC 4 cut(s) 228, 606, 811, 862
AfiI CCNNNNNNNGG 4 cut(s) 86, 87, 168, 465
AflII CTTAAG 1 cut(s) 449
AgeI ACCGGT 1 cut(s) 363
AgsI TTSAA 2 cut(s) 515, 1041
AhdI GACNNNNNGTC 1 cut(s) 367
AjnI CCWGG 3 cut(s) 85, 766, 829
AluBI AGCT 9 cut(s) 404, 434, 647, 710, 760, 785, 982, 1079, 1435
AluI AGCT 9 cut(s) 404, 434, 647, 710, 760, 785, 982, 1079, 1435
Alw21I GWGCWC 4 cut(s) 309, 458, 762, 1002
Alw26I GTCTC 3 cut(s) 154, 184, 190
Alw44I GTGCAC 2 cut(s) 454, 998
AlwI GGATC 1 cut(s) 608
AlwNI CAGNNNCTG 2 cut(s) 982, 1208
AoxI GGCC 1 cut(s) 423
ApaLI GTGCAC 2 cut(s) 454, 998
ApeKI GCWGC 7 cut(s) 431, 434, 639, 782, 982, 1130, 1202
ApoI RAATTY 3 cut(s) 1298, 1324, 1357
ArsI GACNNNNNNTTYG 2 cut(s) 618, 650
AsiGI ACCGGT 1 cut(s) 363
Asp700I GAANNNNTTC 1 cut(s) 1425
AspLEI GCGC 2 cut(s) 124, 140
AspS9I GGNCC 3 cut(s) 361, 833, 1028
AsuHPI GGTGA 1 cut(s) 1024
AvaII GGWCC 3 cut(s) 361, 833, 1028
BaeGI GKGCMC 4 cut(s) 318, 458, 471, 1002
BalI TGGCCA 1 cut(s) 425
BanII GRGCYC 2 cut(s) 174, 762
BbrPI CACGTG 2 cut(s) 472, 839
Bbv12I GWGCWC 4 cut(s) 309, 458, 762, 1002
BbvI GCAGC 7 cut(s) 421, 443, 651, 794, 969, 1117, 1214
BccI CCATC 2 cut(s) 122, 1159
BceAI ACGGC 1 cut(s) 782
BciT130I CCWGG 3 cut(s) 87, 768, 831
BciVI GTATCC 2 cut(s) 58, 1426
BcoDI GTCTC 3 cut(s) 154, 184, 190
BfaI CTAG 2 cut(s) 573, 1053
BfmI CTRYAG 1 cut(s) 786
BfrI CTTAAG 1 cut(s) 449
BfuI GTATCC 2 cut(s) 58, 1426
BisI GCNGC 9 cut(s) 380, 432, 435, 640, 780, 783, 983, 1131, 1203
BlsI GCNGC 9 cut(s) 381, 433, 436, 641, 781, 784, 984, 1132, 1204
Bme1390I CCNGG 3 cut(s) 87, 768, 831
Bme18I GGWCC 3 cut(s) 361, 833, 1028
BmeRI GACNNNNNGTC 1 cut(s) 367
BmgT120I GGNCC 3 cut(s) 361, 833, 1028
BmiI GGNNCC 3 cut(s) 223, 663, 1029
BmrFI CCNGG 3 cut(s) 87, 768, 831
BmrI ACTGGG 1 cut(s) 406
BmsI GCATC 3 cut(s) 13, 587, 1283
BmuI ACTGGG 1 cut(s) 406
BpmI CTGGAG 1 cut(s) 190
Bpu10I CCTNAGC 1 cut(s) 1431
BpuEI CTTGAG 2 cut(s) 971, 1268
BsaAI YACGTR 2 cut(s) 472, 839
BsaJI CCNNGG 5 cut(s) 85, 86, 829, 1031, 1245
BsaWI WCCGGW 1 cut(s) 363
Bsc4I CCNNNNNNNGG 4 cut(s) 86, 87, 168, 465
Bse118I RCCGGY 1 cut(s) 363
Bse1I ACTGG 2 cut(s) 401, 1384
Bse3DI GCAATG 2 cut(s) 617, 745
BseBI CCWGG 3 cut(s) 87, 768, 831
BseDI CCNNGG 5 cut(s) 85, 86, 829, 1031, 1245
BseGI GGATG 4 cut(s) 684, 906, 1115, 1297
BseLI CCNNNNNNNGG 4 cut(s) 86, 87, 168, 465
BseMI GCAATG 2 cut(s) 617, 745
BseMII CTCAG 1 cut(s) 927
BseNI ACTGG 2 cut(s) 401, 1384
BseRI GAGGAG 1 cut(s) 43
BseSI GKGCMC 4 cut(s) 318, 458, 471, 1002
BseXI GCAGC 7 cut(s) 421, 443, 651, 794, 969, 1117, 1214
BseYI CCCAGC 2 cut(s) 80, 600
BsgI GTGCAG 2 cut(s) 1221, 1428
Bsh1236I CGCG 2 cut(s) 122, 138
Bsh1285I CGRYCG 1 cut(s) 346
BshFI GGCC 1 cut(s) 425
BshTI ACCGGT 1 cut(s) 363
BsiEI CGRYCG 1 cut(s) 346
BsiHKAI GWGCWC 4 cut(s) 309, 458, 762, 1002
BsiSI CCGG 2 cut(s) 364, 383
BslFI GGGAC 1 cut(s) 1333
BslI CCNNNNNNNGG 4 cut(s) 86, 87, 168, 465
BsmAI GTCTC 3 cut(s) 154, 184, 190
BsmBI CGTCTC 2 cut(s) 154, 190
BsmFI GGGAC 1 cut(s) 1333
BsmI GAATGC 1 cut(s) 668
BsnI GGCC 1 cut(s) 425
Bsp1286I GDGCHC 7 cut(s) 174, 309, 318, 458, 471, 762, 1002
Bsp143I GATC 1 cut(s) 613
BspACI CCGC 8 cut(s) 157, 162, 329, 346, 372, 380, 459, 780
BspANI GGCC 1 cut(s) 425
BspCNI CTCAG 1 cut(s) 928
BspFNI CGCG 2 cut(s) 122, 138
BspLI GGNNCC 3 cut(s) 223, 663, 1029
BspPI GGATC 1 cut(s) 608
BspQI GCTCTTC 1 cut(s) 239
BspTI CTTAAG 1 cut(s) 449
BsrDI GCAATG 2 cut(s) 617, 745
BsrFI RCCGGY 1 cut(s) 363
BsrI ACTGG 2 cut(s) 401, 1384
BssAI RCCGGY 1 cut(s) 363
BssECI CCNNGG 5 cut(s) 85, 86, 829, 1031, 1245
BssMI GATC 1 cut(s) 613
BssT1I CCWWGG 2 cut(s) 1031, 1245
Bst2UI CCWGG 3 cut(s) 87, 768, 831
Bst4CI ACNGT 6 cut(s) 16, 495, 569, 720, 1105, 1449
Bst6I CTCTTC 1 cut(s) 239
BstAFI CTTAAG 1 cut(s) 449
BstAPI GCANNNNNTGC 5 cut(s) 291, 982, 997, 1199, 1208
BstBAI YACGTR 2 cut(s) 472, 839
BstC8I GCNNGC 1 cut(s) 666
BstDEI CTNAG 2 cut(s) 936, 1431
BstF5I GGATG 4 cut(s) 684, 906, 1115, 1297
BstFNI CGCG 2 cut(s) 122, 138
BstHHI GCGC 2 cut(s) 124, 140
BstKTI GATC 1 cut(s) 616
BstMAI GTCTC 3 cut(s) 154, 184, 190
BstMBI GATC 1 cut(s) 613
BstMCI CGRYCG 1 cut(s) 346
BstNI CCWGG 3 cut(s) 87, 768, 831
BstNSI RCATGY 1 cut(s) 1162
BstSCI CCNGG 3 cut(s) 85, 766, 829
BstSFI CTRYAG 1 cut(s) 786
BstSLI GKGCMC 4 cut(s) 318, 458, 471, 1002
BstUI CGCG 2 cut(s) 122, 138
BstV1I GCAGC 7 cut(s) 421, 443, 651, 794, 969, 1117, 1214
BsuI GTATCC 2 cut(s) 58, 1426
BsuRI GGCC 1 cut(s) 425
BtsCI GGATG 4 cut(s) 684, 906, 1115, 1297
BtsI GCAGTG 1 cut(s) 7
BtsIMutI CAGTG 1 cut(s) 7
Cac8I GCNNGC 1 cut(s) 666
CaiI CAGNNNCTG 2 cut(s) 982, 1208
CfoI GCGC 2 cut(s) 124, 140
Cfr10I RCCGGY 1 cut(s) 363
Cfr13I GGNCC 3 cut(s) 361, 833, 1028
CseI GACGC 1 cut(s) 238
Csp6I GTAC 4 cut(s) 227, 605, 810, 861
CspAI ACCGGT 1 cut(s) 363
CviAII CATG 5 cut(s) 44, 358, 532, 629, 1159
CviQI GTAC 4 cut(s) 227, 605, 810, 861
DdeI CTNAG 2 cut(s) 936, 1431
DpnI GATC 1 cut(s) 615
DpnII GATC 1 cut(s) 613
DrdI GACNNNNNNGTC 1 cut(s) 269
DriI GACNNNNNGTC 1 cut(s) 367
DseDI GACNNNNNNGTC 1 cut(s) 269
EaeI YGGCCR 1 cut(s) 423
Eam1104I CTCTTC 1 cut(s) 239
Eam1105I GACNNNNNGTC 1 cut(s) 367
EarI CTCTTC 1 cut(s) 239
EciI GGCGGA 1 cut(s) 361
Ecl136II GAGCTC 1 cut(s) 760
Eco130I CCWWGG 2 cut(s) 1031, 1245
Eco24I GRGCYC 2 cut(s) 174, 762
Eco32I GATATC 1 cut(s) 301
Eco47I GGWCC 3 cut(s) 361, 833, 1028
Eco53kI GAGCTC 1 cut(s) 760
Eco72I CACGTG 2 cut(s) 472, 839
EcoICRI GAGCTC 1 cut(s) 760
EcoRII CCWGG 3 cut(s) 85, 766, 829
EcoRV GATATC 1 cut(s) 301
EcoT14I CCWWGG 2 cut(s) 1031, 1245
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 174, 762
ErhI CCWWGG 2 cut(s) 1031, 1245
Esp3I CGTCTC 2 cut(s) 154, 190
FaeI CATG 5 cut(s) 47, 361, 535, 632, 1162
FalI AAGNNNNNCTT 2 cut(s) 1051, 1083
FaqI GGGAC 1 cut(s) 1333
FatI CATG 5 cut(s) 43, 357, 531, 628, 1158
FauNDI CATATG 1 cut(s) 1141
Fnu4HI GCNGC 9 cut(s) 380, 432, 435, 640, 780, 783, 983, 1131, 1203
FokI GGATG 4 cut(s) 691, 913, 1122, 1304
FriOI GRGCYC 2 cut(s) 174, 762
Fsp4HI GCNGC 9 cut(s) 380, 432, 435, 640, 780, 783, 983, 1131, 1203
FspBI CTAG 2 cut(s) 573, 1053
GlaI GCGC 2 cut(s) 123, 139
GluI GCNGC 9 cut(s) 380, 432, 435, 640, 780, 783, 983, 1131, 1203
GsaI CCCAGC 2 cut(s) 84, 604
GsuI CTGGAG 1 cut(s) 190
HaeIII GGCC 1 cut(s) 425
HapII CCGG 2 cut(s) 364, 383
HgaI GACGC 1 cut(s) 238
HhaI GCGC 2 cut(s) 124, 140
Hin1II CATG 5 cut(s) 47, 361, 535, 632, 1162
Hin6I GCGC 2 cut(s) 122, 138
HinP1I GCGC 2 cut(s) 122, 138
HincII GTYRAC 3 cut(s) 565, 802, 1171
HindII GTYRAC 3 cut(s) 565, 802, 1171
HinfI GANTC 2 cut(s) 727, 938
HpaI GTTAAC 1 cut(s) 1171
HpaII CCGG 2 cut(s) 364, 383
HphI GGTGA 1 cut(s) 1024
Hpy166II GTNNAC 7 cut(s) 229, 340, 456, 565, 802, 1000, 1171
Hpy188I TCNGA 2 cut(s) 928, 1420
Hpy188III TCNNGA 2 cut(s) 515, 693
Hpy8I GTNNAC 7 cut(s) 229, 340, 456, 565, 802, 1000, 1171
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 3 cut(s) 515, 521, 1402
HpyCH4III ACNGT 6 cut(s) 16, 495, 569, 720, 1105, 1449
HpyCH4IV ACGT 6 cut(s) 264, 471, 804, 838, 923, 1346
HpyF3I CTNAG 2 cut(s) 936, 1431
HpySE526I ACGT 6 cut(s) 264, 471, 804, 838, 923, 1346
Hsp92II CATG 5 cut(s) 47, 361, 535, 632, 1162
HspAI GCGC 2 cut(s) 122, 138
KspAI GTTAAC 1 cut(s) 1171
Kzo9I GATC 1 cut(s) 613
LguI GCTCTTC 1 cut(s) 239
LmnI GCTCC 3 cut(s) 227, 661, 979
Lsp1109I GCAGC 7 cut(s) 421, 443, 651, 794, 969, 1117, 1214
LweI GCATC 3 cut(s) 13, 587, 1283
MaeI CTAG 2 cut(s) 573, 1053
MaeII ACGT 6 cut(s) 264, 471, 804, 838, 923, 1346
MaeIII GTNAC 3 cut(s) 16, 476, 545
MalI GATC 1 cut(s) 615
MboI GATC 1 cut(s) 613
MboII GAAGA 5 cut(s) 109, 256, 814, 881, 905
MfeI CAATTG 2 cut(s) 462, 1182
MhlI GDGCHC 7 cut(s) 174, 309, 318, 458, 471, 762, 1002
MlsI TGGCCA 1 cut(s) 425
MluNI TGGCCA 1 cut(s) 425
MlyI GAGTC 1 cut(s) 947
MmeI TCCRAC 4 cut(s) 177, 517, 656, 1087
Mox20I TGGCCA 1 cut(s) 425
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 1425
MscI TGGCCA 1 cut(s) 425
MseI TTAA 7 cut(s) 258, 450, 885, 954, 1170, 1313, 1388
MslI CAYNNNNRTG 2 cut(s) 595, 1163
Msp20I TGGCCA 1 cut(s) 425
MspA1I CMGCKG 2 cut(s) 434, 785
MspCI CTTAAG 1 cut(s) 449
MspI CCGG 2 cut(s) 364, 383
MspR9I CCNGG 3 cut(s) 87, 768, 831
MunI CAATTG 2 cut(s) 462, 1182
Mva1269I GAATGC 1 cut(s) 668
MvaI CCWGG 3 cut(s) 87, 768, 831
MvnI CGCG 2 cut(s) 122, 138
NdeI CATATG 1 cut(s) 1141
NdeII GATC 1 cut(s) 613
NlaIII CATG 5 cut(s) 47, 361, 535, 632, 1162
NlaIV GGNNCC 3 cut(s) 223, 663, 1029
NmeAIII GCCGAG 2 cut(s) 261, 668
NmuCI GTSAC 1 cut(s) 545
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 1162
PasI CCCWGGG 1 cut(s) 86
PciSI GCTCTTC 1 cut(s) 239
PcsI WCGNNNNNNNCGW 2 cut(s) 180, 477
PctI GAATGC 1 cut(s) 668
PdmI GAANNNNTTC 1 cut(s) 1425
PfeI GAWTC 1 cut(s) 727
PinAI ACCGGT 1 cut(s) 363
PkrI GCNGC 9 cut(s) 381, 433, 436, 641, 781, 784, 984, 1132, 1204
PleI GAGTC 1 cut(s) 946
PmaCI CACGTG 2 cut(s) 472, 839
PmlI CACGTG 2 cut(s) 472, 839
PpsI GAGTC 1 cut(s) 946
Ppu21I YACGTR 2 cut(s) 472, 839
PsiI TTATAA 1 cut(s) 1268
Psp124BI GAGCTC 1 cut(s) 762
Psp6I CCWGG 3 cut(s) 85, 766, 829
PspCI CACGTG 2 cut(s) 472, 839
PspFI CCCAGC 2 cut(s) 80, 600
PspGI CCWGG 3 cut(s) 85, 766, 829
PspN4I GGNNCC 3 cut(s) 223, 663, 1029
PspPI GGNCC 3 cut(s) 361, 833, 1028
PstNI CAGNNNCTG 2 cut(s) 982, 1208
PvuII CAGCTG 2 cut(s) 434, 785
RsaI GTAC 4 cut(s) 228, 606, 811, 862
RsaNI GTAC 4 cut(s) 227, 605, 810, 861
RseI CAYNNNNRTG 2 cut(s) 595, 1163
SacI GAGCTC 1 cut(s) 762
SapI GCTCTTC 1 cut(s) 239
SaqAI TTAA 7 cut(s) 258, 450, 885, 954, 1170, 1313, 1388
SatI GCNGC 9 cut(s) 380, 432, 435, 640, 780, 783, 983, 1131, 1203
Sau3AI GATC 1 cut(s) 613
Sau96I GGNCC 3 cut(s) 361, 833, 1028
SchI GAGTC 1 cut(s) 947
ScrFI CCNGG 3 cut(s) 87, 768, 831
SduI GDGCHC 7 cut(s) 174, 309, 318, 458, 471, 762, 1002
SfaNI GCATC 3 cut(s) 13, 587, 1283
SfcI CTRYAG 1 cut(s) 786
SinI GGWCC 3 cut(s) 361, 833, 1028
SmiMI CAYNNNNRTG 2 cut(s) 595, 1163
SmlI CTYRAG 3 cut(s) 449, 986, 1283
SmoI CTYRAG 3 cut(s) 449, 986, 1283
SsiI CCGC 8 cut(s) 157, 162, 329, 346, 372, 380, 459, 780
SspI AATATT 1 cut(s) 966
SspMI CTAG 2 cut(s) 573, 1053
SstI GAGCTC 1 cut(s) 762
StyD4I CCNGG 3 cut(s) 85, 766, 829
StyI CCWWGG 2 cut(s) 1031, 1245
TaaI ACNGT 6 cut(s) 16, 495, 569, 720, 1105, 1449
TaiI ACGT 6 cut(s) 267, 474, 807, 841, 926, 1349
TaqI TCGA 4 cut(s) 174, 825, 1083, 1335
TauI GCSGC 2 cut(s) 382, 782
TfiI GAWTC 1 cut(s) 727
Tru1I TTAA 7 cut(s) 258, 450, 885, 954, 1170, 1313, 1388
Tru9I TTAA 7 cut(s) 258, 450, 885, 954, 1170, 1313, 1388
TscAI CASTG 1 cut(s) 14
TseFI GTSAC 1 cut(s) 545
TseI GCWGC 7 cut(s) 431, 434, 639, 782, 982, 1130, 1202
Tsp45I GTSAC 1 cut(s) 545
TspDTI ATGAA 3 cut(s) 534, 600, 1335
TspGWI ACGGA 3 cut(s) 136, 172, 214
TspRI CASTG 1 cut(s) 14
Vha464I CTTAAG 1 cut(s) 449
VneI GTGCAC 2 cut(s) 454, 998
VpaK11BI GGWCC 3 cut(s) 361, 833, 1028
XapI RAATTY 3 cut(s) 1298, 1324, 1357
XceI RCATGY 1 cut(s) 1162
XmnI GAANNNNTTC 1 cut(s) 1425
XspI CTAG 2 cut(s) 573, 1053
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.