MD05G1203500.v1.1

pfkB family carbohydrate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009506
Physical Location & Seq
Forward (+)
33355362 .. 33358599
3238 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1203500.v1.1.491

Sequence Viewer

Length: 1473 bp
ATGCATCACTGCATCGTAACCCTGAATCCCCCAATCCCACACCATGTATCCTCCCCTCACTCCCACCTGATATTTCCCCACAGCCCCAGGCTGACCCAATCCCAATCCCACCTCATTTTCGCGCCAGTTTGCGTTCGCGCCCATTCCGTCTCCAACCGCACCGCCAGAGCGCTCGATGTTTCCGTCTCCAAACACTCCCCTAACTCCAGCGGCCCCAATGGCTCCGTGCACGATTGGAAGCCAAAGACCGTCGGCGTTAAAAACGTAGATGTTGCTACTCTCGGCAATCTCTGCGTGGATATCGTCCTCAGTGTGCCCAAATTGCCCCCGCCCGATGTCCGCGACCGCAAAGCTTACATGGACCTGTTATCCGCCTCCCCGCCGGATAAGCAATACTGGGAAGCTGGTGGAAACTGCAATATGGCCATAGCAGCTGCAAGACTAGGACTTCATTGCATTGCGATTGGGCATGTGGGTAACGAAGTATATGGACAGTTCCTTTTAGATGTTCTTCAAGATGAAGGAATTAGCATGGTTGGAATGAGTGACAACAATACCAATGTTGACAGTTCAGCTGCATCATATGAAACACTTTTGTGCTGGGTTCTTGTCGATCCATTGCAAAGACATGGTTTTTGCAGCCGAGCTGATTTCAGTAAGGAGCCTGCATTCAGTTGGATGAGCAAATTATCTGCACAAGTAAAGACTGCTATAAAACAGTCAAAGATTCTGTTCTGCAATGGTTATGGCTTTGATGAACTCTCTCCAGGTGTAATAGCTGCAGCTGTAGAGTATGCCGTCGAAGCTGGTACATCAATTTTCTTCGACCCTGGACCACGTGGTAAAAGCCTTTCTGCTGGTACAACAGAAGAACGCGAAGCACTTAACCTGTTGTTGAGGATGAGTGATGTTCTTCTTCTAACTTCTGATGAGGCTGAGTCATTGACGGGCATTAAAAACCCAGTATTAGCAGGGCAGGAGCTGCTCAAGCAAGGGGTGCTCACAAAATGGGTGATTGTCAAAATGGGTCCAAGGGGTTCAATTCTAATAACTAGGTCAAGTATTTCTTGCGCACCAGCGTTTGAGGTAAATGTCGTAGACACTGTTGGGTGTGGAGATAGTTTCGTGGCTGCGATTGCATATGGTTTTATCCACAATATGCCGAAGGTTAACACATTAGCAGTTGCAAATGCTGTAGGTGCTGCAACCGCCACAGGTTGTGGTGCCGGTAGGAATGTGGCAAACTTGGAGAAAGTAATAGAACTGATAAGTGCATCTAATCTCAAGCAGGATGACAAATTTTGGAATGAGTTAATTGATGAAAATTTTGGTGTCGAGGAAATTACATCTCTCTCAAATTTGGTCGTAGATGGAAGTGACAACCGGTTAAATTGTGTATCCTTGCAGAAGGTGGTATCCGAATTGCTTCCTAAGCTTAAATCTGCACAGTTCAAAAGAAAAGTGCCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

491

Amino Acids

52.64

Weight (kDa)

6.43

Isoelectric Point (pI)

34.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 135 - 410 5.9e-34 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1072
AccB1I GGYRCC 1 cut(s) 1223
AccI GTMKAC 1 cut(s) 1098
AccII CGCG 4 cut(s) 122, 138, 342, 876
AciI CCGC 9 cut(s) 157, 162, 210, 329, 340, 346, 372, 380, 1209
AclWI GGATC 1 cut(s) 608
AcoI YGGCCR 1 cut(s) 423
AcsI RAATTY 3 cut(s) 1298, 1324, 1357
AcvI CACGTG 1 cut(s) 839
AfaI GTAC 2 cut(s) 811, 862
AfeI AGCGCT 1 cut(s) 171
AgeI ACCGGT 1 cut(s) 1383
AgsI TTSAA 3 cut(s) 515, 1041, 1453
AjnI CCWGG 3 cut(s) 86, 766, 829
AleI CACNNNNGTG 1 cut(s) 595
Alw21I GWGCWC 2 cut(s) 231, 1002
Alw26I GTCTC 2 cut(s) 154, 190
Alw44I GTGCAC 1 cut(s) 227
AlwI GGATC 1 cut(s) 608
AlwNI CAGNNNCTG 1 cut(s) 982
Aor51HI AGCGCT 1 cut(s) 171
AoxI GGCC 2 cut(s) 211, 423
ApaLI GTGCAC 1 cut(s) 227
ApeKI GCWGC 9 cut(s) 431, 434, 575, 639, 779, 782, 982, 1130, 1202
ApoI RAATTY 3 cut(s) 1298, 1324, 1357
ArsI GACNNNNNNTTYG 2 cut(s) 618, 650
AsiGI ACCGGT 1 cut(s) 1383
Asp700I GAANNNNTTC 1 cut(s) 1425
AspLEI GCGC 4 cut(s) 124, 140, 172, 1073
AspS9I GGNCC 4 cut(s) 212, 361, 833, 1028
AsuHPI GGTGA 1 cut(s) 1024
AvaII GGWCC 3 cut(s) 361, 833, 1028
BaeGI GKGCMC 2 cut(s) 231, 318
BalI TGGCCA 1 cut(s) 425
BanI GGYRCC 1 cut(s) 1223
BbrPI CACGTG 1 cut(s) 839
Bbv12I GWGCWC 2 cut(s) 231, 1002
BbvI GCAGC 9 cut(s) 421, 443, 562, 651, 766, 794, 969, 1117, 1189
BccI CCATC 1 cut(s) 1364
BceAI ACGGC 1 cut(s) 782
BciT130I CCWGG 3 cut(s) 88, 768, 831
BciVI GTATCC 3 cut(s) 58, 1408, 1426
BcoDI GTCTC 2 cut(s) 154, 190
BfaI CTAG 2 cut(s) 443, 1053
BfmI CTRYAG 3 cut(s) 780, 786, 1194
BfoI RGCGCY 1 cut(s) 173
BfuI GTATCC 3 cut(s) 58, 1408, 1426
BglI GCCNNNNNGGC 1 cut(s) 219
Bme1390I CCNGG 3 cut(s) 88, 768, 831
Bme18I GGWCC 3 cut(s) 361, 833, 1028
BmgT120I GGNCC 4 cut(s) 212, 361, 833, 1028
BmiI GGNNCC 5 cut(s) 214, 223, 663, 1029, 1225
BmrFI CCNGG 3 cut(s) 88, 768, 831
BmrI ACTGGG 2 cut(s) 406, 956
BmsI GCATC 4 cut(s) 13, 21, 587, 1283
BmuI ACTGGG 2 cut(s) 406, 956
BpmI CTGGAG 2 cut(s) 190, 750
Bpu10I CCTNAGC 1 cut(s) 1431
BpuEI CTTGAG 2 cut(s) 971, 1268
BsaAI YACGTR 1 cut(s) 839
BsaJI CCNNGG 3 cut(s) 86, 829, 1031
BsaWI WCCGGW 1 cut(s) 1383
Bse118I RCCGGY 2 cut(s) 1226, 1383
Bse1I ACTGG 3 cut(s) 125, 401, 962
Bse3DI GCAATG 4 cut(s) 451, 456, 617, 745
BseBI CCWGG 3 cut(s) 88, 768, 831
BseDI CCNNGG 3 cut(s) 86, 829, 1031
BseGI GGATG 3 cut(s) 684, 906, 1297
BseMI GCAATG 4 cut(s) 451, 456, 617, 745
BseMII CTCAG 2 cut(s) 322, 927
BseNI ACTGG 3 cut(s) 125, 401, 962
BseSI GKGCMC 2 cut(s) 231, 318
BseXI GCAGC 9 cut(s) 421, 443, 562, 651, 766, 794, 969, 1117, 1189
BseYI CCCAGC 1 cut(s) 600
BsgI GTGCAG 2 cut(s) 678, 1428
Bsh1236I CGCG 4 cut(s) 122, 138, 342, 876
Bsh1285I CGRYCG 1 cut(s) 346
BshFI GGCC 2 cut(s) 213, 425
BshNI GGYRCC 1 cut(s) 1223
BshTI ACCGGT 1 cut(s) 1383
BsiEI CGRYCG 1 cut(s) 346
BsiHKAI GWGCWC 2 cut(s) 231, 1002
BsiSI CCGG 3 cut(s) 383, 1227, 1384
BsmAI GTCTC 2 cut(s) 154, 190
BsmBI CGTCTC 2 cut(s) 154, 190
BsmI GAATGC 1 cut(s) 668
BsnI GGCC 2 cut(s) 213, 425
Bsp1286I GDGCHC 3 cut(s) 231, 318, 1002
Bsp143I GATC 1 cut(s) 613
BspACI CCGC 9 cut(s) 157, 162, 210, 329, 340, 346, 372, 380, 1209
BspANI GGCC 2 cut(s) 213, 425
BspCNI CTCAG 2 cut(s) 321, 928
BspFNI CGCG 4 cut(s) 122, 138, 342, 876
BspLI GGNNCC 5 cut(s) 214, 223, 663, 1029, 1225
BspMAI CTGCAG 1 cut(s) 784
BspPI GGATC 1 cut(s) 608
BspT107I GGYRCC 1 cut(s) 1223
BsrDI GCAATG 4 cut(s) 451, 456, 617, 745
BsrFI RCCGGY 2 cut(s) 1226, 1383
BsrI ACTGG 3 cut(s) 125, 401, 962
BssAI RCCGGY 2 cut(s) 1226, 1383
BssECI CCNNGG 3 cut(s) 86, 829, 1031
BssMI GATC 1 cut(s) 613
BssT1I CCWWGG 1 cut(s) 1031
Bst2UI CCWGG 3 cut(s) 88, 768, 831
Bst4CI ACNGT 6 cut(s) 250, 495, 569, 720, 1105, 1449
BstAPI GCANNNNNTGC 3 cut(s) 291, 982, 997
BstBAI YACGTR 1 cut(s) 839
BstC8I GCNNGC 1 cut(s) 666
BstDEI CTNAG 3 cut(s) 308, 936, 1431
BstF5I GGATG 3 cut(s) 684, 906, 1297
BstFNI CGCG 4 cut(s) 122, 138, 342, 876
BstH2I RGCGCY 1 cut(s) 173
BstHHI GCGC 4 cut(s) 124, 140, 172, 1073
BstKTI GATC 1 cut(s) 616
BstMAI GTCTC 2 cut(s) 154, 190
BstMBI GATC 1 cut(s) 613
BstMCI CGRYCG 1 cut(s) 346
BstNI CCWGG 3 cut(s) 88, 768, 831
BstNSI RCATGY 1 cut(s) 473
BstSCI CCNGG 3 cut(s) 86, 766, 829
BstSFI CTRYAG 3 cut(s) 780, 786, 1194
BstSLI GKGCMC 2 cut(s) 231, 318
BstUI CGCG 4 cut(s) 122, 138, 342, 876
BstV1I GCAGC 9 cut(s) 421, 443, 562, 651, 766, 794, 969, 1117, 1189
BsuI GTATCC 3 cut(s) 58, 1408, 1426
BsuRI GGCC 2 cut(s) 213, 425
BtsCI GGATG 3 cut(s) 684, 906, 1297
BtsI GCAGTG 1 cut(s) 7
BtsIMutI CAGTG 3 cut(s) 7, 316, 1101
Cac8I GCNNGC 1 cut(s) 666
CaiI CAGNNNCTG 1 cut(s) 982
CfoI GCGC 4 cut(s) 124, 140, 172, 1073
Cfr10I RCCGGY 2 cut(s) 1226, 1383
Cfr13I GGNCC 4 cut(s) 212, 361, 833, 1028
Csp6I GTAC 2 cut(s) 810, 861
CspAI ACCGGT 1 cut(s) 1383
CviAII CATG 5 cut(s) 44, 358, 470, 532, 629
CviQI GTAC 2 cut(s) 810, 861
DdeI CTNAG 3 cut(s) 308, 936, 1431
DpnI GATC 1 cut(s) 615
DpnII GATC 1 cut(s) 613
EaeI YGGCCR 1 cut(s) 423
EciI GGCGGA 1 cut(s) 361
Eco130I CCWWGG 1 cut(s) 1031
Eco32I GATATC 1 cut(s) 301
Eco47I GGWCC 3 cut(s) 361, 833, 1028
Eco47III AGCGCT 1 cut(s) 171
Eco72I CACGTG 1 cut(s) 839
EcoRII CCWGG 3 cut(s) 86, 766, 829
EcoRV GATATC 1 cut(s) 301
EcoT14I CCWWGG 1 cut(s) 1031
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 1031
Esp3I CGTCTC 2 cut(s) 154, 190
FaeI CATG 5 cut(s) 47, 361, 473, 535, 632
FalI AAGNNNNNCTT 2 cut(s) 1051, 1083
FatI CATG 5 cut(s) 43, 357, 469, 531, 628
FauI CCCGC 2 cut(s) 336, 387
FauNDI CATATG 2 cut(s) 583, 1141
FblI GTMKAC 1 cut(s) 1098
FokI GGATG 3 cut(s) 691, 913, 1304
FspBI CTAG 2 cut(s) 443, 1053
FspI TGCGCA 1 cut(s) 1072
GlaI GCGC 4 cut(s) 123, 139, 171, 1072
GsaI CCCAGC 1 cut(s) 604
GsuI CTGGAG 2 cut(s) 190, 750
HaeII RGCGCY 1 cut(s) 173
HaeIII GGCC 2 cut(s) 213, 425
HapII CCGG 3 cut(s) 383, 1227, 1384
HhaI GCGC 4 cut(s) 124, 140, 172, 1073
Hin1II CATG 5 cut(s) 47, 361, 473, 535, 632
Hin6I GCGC 4 cut(s) 122, 138, 170, 1071
HinP1I GCGC 4 cut(s) 122, 138, 170, 1071
HincII GTYRAC 2 cut(s) 565, 1171
HindII GTYRAC 2 cut(s) 565, 1171
HindIII AAGCTT 2 cut(s) 351, 1433
HinfI GANTC 3 cut(s) 25, 727, 938
HpaI GTTAAC 1 cut(s) 1171
HpaII CCGG 3 cut(s) 383, 1227, 1384
HphI GGTGA 1 cut(s) 1024
Hpy166II GTNNAC 4 cut(s) 229, 565, 1099, 1171
Hpy188I TCNGA 2 cut(s) 928, 1420
Hpy188III TCNNGA 1 cut(s) 515
Hpy8I GTNNAC 4 cut(s) 229, 565, 1099, 1171
Hpy99I CGWCG 2 cut(s) 254, 803
HpyAV CCTTC 3 cut(s) 515, 1159, 1402
HpyCH4III ACNGT 6 cut(s) 250, 495, 569, 720, 1105, 1449
HpyCH4IV ACGT 2 cut(s) 264, 838
HpyF3I CTNAG 3 cut(s) 308, 936, 1431
HpySE526I ACGT 2 cut(s) 264, 838
Hsp92II CATG 5 cut(s) 47, 361, 473, 535, 632
HspAI GCGC 4 cut(s) 122, 138, 170, 1071
KspAI GTTAAC 1 cut(s) 1171
Kzo9I GATC 1 cut(s) 613
LmnI GCTCC 3 cut(s) 227, 661, 979
Lsp1109I GCAGC 9 cut(s) 421, 443, 562, 651, 766, 794, 969, 1117, 1189
LweI GCATC 4 cut(s) 13, 21, 587, 1283
MaeI CTAG 2 cut(s) 443, 1053
MaeII ACGT 2 cut(s) 264, 838
MaeIII GTNAC 4 cut(s) 16, 476, 545, 1376
MalI GATC 1 cut(s) 615
MboI GATC 1 cut(s) 613
MboII GAAGA 5 cut(s) 503, 814, 881, 905, 908
MhlI GDGCHC 3 cut(s) 231, 318, 1002
MlsI TGGCCA 1 cut(s) 425
MluNI TGGCCA 1 cut(s) 425
MlyI GAGTC 1 cut(s) 947
MmeI TCCRAC 3 cut(s) 177, 517, 656
MnlI CCTC 9 cut(s) 61, 66, 122, 317, 385, 891, 925, 1078, 1330
Mox20I TGGCCA 1 cut(s) 425
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 1425
MscI TGGCCA 1 cut(s) 425
MseI TTAA 7 cut(s) 258, 885, 954, 1170, 1313, 1388, 1437
MslI CAYNNNNRTG 1 cut(s) 595
Msp20I TGGCCA 1 cut(s) 425
MspA1I CMGCKG 4 cut(s) 210, 434, 575, 785
MspI CCGG 3 cut(s) 383, 1227, 1384
MspR9I CCNGG 3 cut(s) 88, 768, 831
Mva1269I GAATGC 1 cut(s) 668
MvaI CCWGG 3 cut(s) 88, 768, 831
MvnI CGCG 4 cut(s) 122, 138, 342, 876
NdeI CATATG 2 cut(s) 583, 1141
NdeII GATC 1 cut(s) 613
NlaIII CATG 5 cut(s) 47, 361, 473, 535, 632
NlaIV GGNNCC 5 cut(s) 214, 223, 663, 1029, 1225
NmeAIII GCCGAG 2 cut(s) 261, 668
NmuCI GTSAC 2 cut(s) 545, 1376
NsbI TGCGCA 1 cut(s) 1072
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 473
OliI CACNNNNGTG 1 cut(s) 595
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PctI GAATGC 1 cut(s) 668
PdmI GAANNNNTTC 1 cut(s) 1425
PfeI GAWTC 2 cut(s) 25, 727
PinAI ACCGGT 1 cut(s) 1383
PleI GAGTC 1 cut(s) 946
PmaCI CACGTG 1 cut(s) 839
PmlI CACGTG 1 cut(s) 839
PpsI GAGTC 1 cut(s) 946
Ppu21I YACGTR 1 cut(s) 839
Psp6I CCWGG 3 cut(s) 86, 766, 829
PspCI CACGTG 1 cut(s) 839
PspFI CCCAGC 1 cut(s) 600
PspGI CCWGG 3 cut(s) 86, 766, 829
PspN4I GGNNCC 5 cut(s) 214, 223, 663, 1029, 1225
PspPI GGNCC 4 cut(s) 212, 361, 833, 1028
PstI CTGCAG 1 cut(s) 784
PstNI CAGNNNCTG 1 cut(s) 982
PvuII CAGCTG 3 cut(s) 434, 575, 785
RsaI GTAC 2 cut(s) 811, 862
RsaNI GTAC 2 cut(s) 810, 861
RseI CAYNNNNRTG 1 cut(s) 595
SaqAI TTAA 7 cut(s) 258, 885, 954, 1170, 1313, 1388, 1437
Sau3AI GATC 1 cut(s) 613
Sau96I GGNCC 4 cut(s) 212, 361, 833, 1028
SchI GAGTC 1 cut(s) 947
ScrFI CCNGG 3 cut(s) 88, 768, 831
SduI GDGCHC 3 cut(s) 231, 318, 1002
SfaNI GCATC 4 cut(s) 13, 21, 587, 1283
SfcI CTRYAG 3 cut(s) 780, 786, 1194
SinI GGWCC 3 cut(s) 361, 833, 1028
SmiMI CAYNNNNRTG 1 cut(s) 595
SmlI CTYRAG 2 cut(s) 986, 1283
SmoI CTYRAG 2 cut(s) 986, 1283
SsiI CCGC 9 cut(s) 157, 162, 210, 329, 340, 346, 372, 380, 1209
SspMI CTAG 2 cut(s) 443, 1053
StyD4I CCNGG 3 cut(s) 86, 766, 829
StyI CCWWGG 1 cut(s) 1031
TaaI ACNGT 6 cut(s) 250, 495, 569, 720, 1105, 1449
TaiI ACGT 2 cut(s) 267, 841
TaqI TCGA 5 cut(s) 174, 612, 801, 825, 1335
TauI GCSGC 1 cut(s) 213
TfiI GAWTC 2 cut(s) 25, 727
Tru1I TTAA 7 cut(s) 258, 885, 954, 1170, 1313, 1388, 1437
Tru9I TTAA 7 cut(s) 258, 885, 954, 1170, 1313, 1388, 1437
TscAI CASTG 3 cut(s) 14, 316, 1108
TseFI GTSAC 2 cut(s) 545, 1376
TseI GCWGC 9 cut(s) 431, 434, 575, 639, 779, 782, 982, 1130, 1202
Tsp45I GTSAC 2 cut(s) 545, 1376
TspDTI ATGAA 5 cut(s) 440, 534, 600, 771, 1335
TspGWI ACGGA 3 cut(s) 136, 172, 214
TspRI CASTG 3 cut(s) 14, 316, 1108
VneI GTGCAC 1 cut(s) 227
VpaK11BI GGWCC 3 cut(s) 361, 833, 1028
XapI RAATTY 3 cut(s) 1298, 1324, 1357
XceI RCATGY 1 cut(s) 473
XmiI GTMKAC 1 cut(s) 1098
XmnI GAANNNNTTC 1 cut(s) 1425
XspI CTAG 2 cut(s) 443, 1053
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.