RLG00000012326

pfkB family carbohydrate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009506
Physical Location & Seq
Reverse (-)
17496884 .. 17499774
2891 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012326

Sequence Viewer

Length: 1419 bp
ATGCACACCATAACCCTTAAAACCCCAATCCCCTACCATGTACCCTCCCTTAAGTCCCACTCCATCTTCCCCCAAAACCCTAGACTAACCCGACCCCACCTCATTTTCCCGCCAGTTCACCACCGCCCCCACTCTCACTCCTCCGTCACCAAAGGCCTCGGCATCTCCGTCCCCAACTCCACCCCCGGCGCTCCCAACGGCTCCCTCCACTCCAAAAACCTCGCCGTTAAAGACATCGACGTCGCAACTCTCGGCAATCTCTGCGTCGACATCGTCCTCAACGTCCCCCAGTTGCCGCCGCCCGATGCCGACCGCCGCAAAGCTTACATGGACCGCCTATCCGCCTCGCCGCCCGATAAGAAATACTGGGAAGCTGGTGGCAACTGCAATATGGCTATAGCAGCTGCCAGGTTGGGCCTACGCTGCAATGCAATTGGCCATGTGGGTAATGAAGTGTATGGGCAGTTCTTATTAGATGTTCTTCGTGATGAAGGAATCGGTATGGTTGGGATGAATGAGGATACTGATGCTGACAGTTCAAGTGCTCCTTCGTATGAGACGCTTTTGTGCTGGGTTCTTGTGGATCAGTTGCAAAGACATGGCTTTTGTAGTCGAGCTGATTTCAGTAAGGAGCCTGCATTCAGTTGGTTGACCAAATTGTCCGTACAAGTAAAGACGGCGATAAATCAGTCAAAGATCCTGTTCTGTAATGGTTATGGCTTTGATGAGCTCTCTCCTGCTGTAATAGCCTCAGCTGTCGAGTATGCCGTTGAAGTTGGAACAGCAATTTTTTTCGACCCTGGACCCCGCGGAAAGGGCCTCTCTGCTGGTACACCTGAAGAACAAGGAGCACTTAACCTGTTGTTGAGGATGAGTGATGCCGAGTCATTGACTGGCATTAAAAACCCGATATTAGCAGGGCAGCAGTTGCTCAAGCAAGGGGTGCAAACAAAATGGGTGATTGTTAAAATGGGGCCAAAGGGTTCAATTCTCATAACCAAGTCTGGCATCTCTTGTGCACCAGCATTCAAGGTAAAGGTTGTGGACACTGTTGGATGCGGAGATAGTTTTGTAGCTGCCATTGCATATGGTTTCATCCACAATATTCCAATGGTAAACACATTAGCAATTGCAAATGCAGTGGGTGCTGCAACCGCCATGGGTTGTGGTGCTGGTAGGAATGTGGCAACATTGGATAAAGTACTTGAAATTGTGAAAGCTTCGAATCTTAATGAGGATGACGAATTTTGGAATGAAATATTTGATGAAAATTTGGGTGTTCAGGAAATTCCTGCTCTCTCAAAATTGGTCATTAATGGAAGTGACAGTGAGCTGAATAGCGTCCCTTTGCAGAAGGTTGTATCTGAATTGCTTCCTAAGCTCAAATCTGCAGAGCTAGAGAGAAAAGTGCCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

473

Amino Acids

50.57

Weight (kDa)

6.46

Isoelectric Point (pI)

31.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 122 - 391 7.9e-30 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 239, 658
AatII GACGTC 1 cut(s) 243
AccI GTMKAC 1 cut(s) 267
AccII CGCG 1 cut(s) 810
AclWI GGATC 2 cut(s) 591, 691
AcoI YGGCCR 1 cut(s) 436
AcsI RAATTY 3 cut(s) 1244, 1270, 1287
AcuI CTGAAG 1 cut(s) 858
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 4 cut(s) 42, 666, 832, 1203
AfiI CCNNNNNNNGG 1 cut(s) 814
AflII CTTAAG 1 cut(s) 50
AgsI TTSAA 5 cut(s) 540, 773, 987, 1030, 1208
AjnI CCWGG 2 cut(s) 407, 799
Alw21I GWGCWC 4 cut(s) 547, 732, 853, 1021
Alw26I GTCTC 1 cut(s) 551
Alw44I GTGCAC 1 cut(s) 1017
AlwI GGATC 2 cut(s) 591, 691
AoxI GGCC 5 cut(s) 154, 415, 436, 817, 974
ApaLI GTGCAC 1 cut(s) 1017
ApeKI GCWGC 6 cut(s) 401, 404, 423, 922, 1076, 1148
ApoI RAATTY 3 cut(s) 1244, 1270, 1287
ArsI GACNNNNNNTTYG 2 cut(s) 588, 620
AseI ATTAAT 1 cut(s) 1314
Asp700I GAANNNNTTC 1 cut(s) 1371
AspLEI GCGC 1 cut(s) 191
AspS9I GGNCC 5 cut(s) 331, 415, 803, 817, 974
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 3 cut(s) 110, 139, 970
AsuII TTCGAA 1 cut(s) 1223
AvaII GGWCC 2 cut(s) 331, 803
BaeGI GKGCMC 1 cut(s) 1021
BalI TGGCCA 1 cut(s) 438
BanII GRGCYC 1 cut(s) 732
Bbv12I GWGCWC 4 cut(s) 547, 732, 853, 1021
BbvCI CCTCAGC 1 cut(s) 751
BbvI GCAGC 6 cut(s) 391, 410, 413, 934, 1063, 1135
BccI CCATC 1 cut(s) 71
BceAI ACGGC 4 cut(s) 209, 214, 693, 752
BciT130I CCWGG 2 cut(s) 409, 801
BciVI GTATCC 1 cut(s) 514
BcnI CCSGG 1 cut(s) 186
BcoDI GTCTC 1 cut(s) 551
BfaI CTAG 2 cut(s) 81, 1397
BfmI CTRYAG 2 cut(s) 396, 1389
BfoI RGCGCY 1 cut(s) 192
BfrI CTTAAG 1 cut(s) 50
BfuI GTATCC 1 cut(s) 514
BmcAI AGTACT 1 cut(s) 1203
Bme1390I CCNGG 3 cut(s) 186, 409, 801
Bme18I GGWCC 2 cut(s) 331, 803
BmgT120I GGNCC 5 cut(s) 331, 415, 803, 817, 974
BmiI GGNNCC 4 cut(s) 202, 633, 805, 975
BmrFI CCNGG 3 cut(s) 186, 409, 801
BmrI ACTGGG 2 cut(s) 283, 376
BmsI GCATC 6 cut(s) 171, 295, 517, 868, 1017, 1046
BmuI ACTGGG 2 cut(s) 283, 376
Bpu10I CCTNAGC 2 cut(s) 751, 1377
Bpu14I TTCGAA 1 cut(s) 1223
BpuEI CTTGAG 1 cut(s) 917
BpuMI CCSGG 1 cut(s) 186
BsaHI GRCGYC 1 cut(s) 240
BsaJI CCNNGG 5 cut(s) 157, 184, 799, 808, 1158
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 1 cut(s) 814
Bse1I ACTGG 4 cut(s) 113, 289, 371, 898
Bse3DI GCAATG 2 cut(s) 433, 1080
BseBI CCWGG 2 cut(s) 409, 801
BseDI CCNNGG 5 cut(s) 157, 184, 799, 808, 1158
BseGI GGATG 5 cut(s) 516, 876, 1061, 1095, 1243
BseLI CCNNNNNNNGG 1 cut(s) 814
BseMI GCAATG 2 cut(s) 433, 1080
BseMII CTCAG 1 cut(s) 765
BseNI ACTGG 4 cut(s) 113, 289, 371, 898
BseRI GAGGAG 1 cut(s) 130
BseSI GKGCMC 1 cut(s) 1021
BseXI GCAGC 6 cut(s) 391, 410, 413, 934, 1063, 1135
BseYI CCCAGC 1 cut(s) 570
Bsh1236I CGCG 1 cut(s) 810
Bsh1285I CGRYCG 1 cut(s) 313
BshFI GGCC 5 cut(s) 156, 417, 438, 819, 976
BsiEI CGRYCG 1 cut(s) 313
BsiHKAI GWGCWC 4 cut(s) 547, 732, 853, 1021
BsiSI CCGG 1 cut(s) 186
BslFI GGGAC 4 cut(s) 40, 155, 269, 1328
BslI CCNNNNNNNGG 1 cut(s) 814
BsmAI GTCTC 1 cut(s) 551
BsmBI CGTCTC 1 cut(s) 551
BsmFI GGGAC 4 cut(s) 40, 155, 269, 1328
BsmI GAATGC 2 cut(s) 638, 1025
BsnI GGCC 5 cut(s) 156, 417, 438, 819, 976
Bsp119I TTCGAA 1 cut(s) 1223
Bsp1286I GDGCHC 4 cut(s) 547, 732, 853, 1021
Bsp143I GATC 2 cut(s) 583, 696
Bsp19I CCATGG 1 cut(s) 1158
BspANI GGCC 5 cut(s) 156, 417, 438, 819, 976
BspCNI CTCAG 1 cut(s) 764
BspFNI CGCG 1 cut(s) 810
BspLI GGNNCC 4 cut(s) 202, 633, 805, 975
BspMAI CTGCAG 1 cut(s) 1393
BspPI GGATC 2 cut(s) 591, 691
BspT104I TTCGAA 1 cut(s) 1223
BspTI CTTAAG 1 cut(s) 50
BsrDI GCAATG 2 cut(s) 433, 1080
BsrI ACTGG 4 cut(s) 113, 289, 371, 898
BssECI CCNNGG 5 cut(s) 157, 184, 799, 808, 1158
BssMI GATC 2 cut(s) 583, 696
BssNI GRCGYC 1 cut(s) 240
BssT1I CCWWGG 1 cut(s) 1158
Bst2UI CCWGG 2 cut(s) 409, 801
Bst4CI ACNGT 3 cut(s) 536, 1051, 1328
BstACI GRCGYC 1 cut(s) 240
BstAFI CTTAAG 1 cut(s) 50
BstAPI GCANNNNNTGC 4 cut(s) 261, 928, 943, 1145
BstBI TTCGAA 1 cut(s) 1223
BstC8I GCNNGC 1 cut(s) 636
BstDEI CTNAG 2 cut(s) 751, 1377
BstDSI CCRYGG 2 cut(s) 808, 1158
BstF5I GGATG 5 cut(s) 516, 876, 1061, 1095, 1243
BstFNI CGCG 1 cut(s) 810
BstH2I RGCGCY 1 cut(s) 192
BstHHI GCGC 1 cut(s) 191
BstKTI GATC 2 cut(s) 586, 699
BstMAI GTCTC 1 cut(s) 551
BstMBI GATC 2 cut(s) 583, 696
BstMCI CGRYCG 1 cut(s) 313
BstNI CCWGG 2 cut(s) 409, 801
BstSCI CCNGG 3 cut(s) 184, 407, 799
BstSFI CTRYAG 2 cut(s) 396, 1389
BstSLI GKGCMC 1 cut(s) 1021
BstUI CGCG 1 cut(s) 810
BstV1I GCAGC 6 cut(s) 391, 410, 413, 934, 1063, 1135
BstX2I RGATCY 1 cut(s) 696
BstYI RGATCY 1 cut(s) 696
BsuI GTATCC 1 cut(s) 514
BsuRI GGCC 5 cut(s) 156, 417, 438, 819, 976
BtgI CCRYGG 2 cut(s) 808, 1158
BtsCI GGATG 5 cut(s) 516, 876, 1061, 1095, 1243
BtsI GCAGTG 1 cut(s) 1146
BtsIMutI CAGTG 3 cut(s) 1047, 1146, 1333
Cac8I GCNNGC 1 cut(s) 636
CfoI GCGC 1 cut(s) 191
Cfr13I GGNCC 5 cut(s) 331, 415, 803, 817, 974
Cfr42I CCGCGG 1 cut(s) 811
CseI GACGC 3 cut(s) 253, 568, 1330
Csp6I GTAC 4 cut(s) 41, 665, 831, 1202
CspCI CAANNNNNGTGG 2 cut(s) 1122, 1157
CviAII CATG 6 cut(s) 38, 328, 440, 599, 1159, 1413
CviQI GTAC 4 cut(s) 41, 665, 831, 1202
DdeI CTNAG 2 cut(s) 751, 1377
DpnI GATC 2 cut(s) 585, 698
DpnII GATC 2 cut(s) 583, 696
DrdI GACNNNNNNGTC 2 cut(s) 239, 658
DseDI GACNNNNNNGTC 2 cut(s) 239, 658
EaeI YGGCCR 1 cut(s) 436
EciI GGCGGA 1 cut(s) 331
Ecl136II GAGCTC 1 cut(s) 730
Eco130I CCWWGG 1 cut(s) 1158
Eco147I AGGCCT 1 cut(s) 156
Eco24I GRGCYC 1 cut(s) 732
Eco47I GGWCC 2 cut(s) 331, 803
Eco53kI GAGCTC 1 cut(s) 730
Eco57I CTGAAG 1 cut(s) 858
EcoICRI GAGCTC 1 cut(s) 730
EcoO109I RGGNCCY 1 cut(s) 817
EcoRII CCWGG 2 cut(s) 407, 799
EcoT14I CCWWGG 1 cut(s) 1158
EcoT38I GRGCYC 1 cut(s) 732
ErhI CCWWGG 1 cut(s) 1158
Esp3I CGTCTC 1 cut(s) 551
FaeI CATG 6 cut(s) 41, 331, 443, 602, 1162, 1416
FalI AAGNNNNNCTT 4 cut(s) 532, 564, 837, 869
FaqI GGGAC 4 cut(s) 40, 155, 269, 1328
FatI CATG 6 cut(s) 37, 327, 439, 598, 1158, 1412
FauI CCCGC 2 cut(s) 117, 815
FauNDI CATATG 1 cut(s) 1087
FblI GTMKAC 1 cut(s) 267
FokI GGATG 5 cut(s) 523, 883, 1068, 1082, 1250
FriOI GRGCYC 1 cut(s) 732
FspBI CTAG 2 cut(s) 81, 1397
GlaI GCGC 1 cut(s) 190
GsaI CCCAGC 1 cut(s) 574
HaeII RGCGCY 1 cut(s) 192
HaeIII GGCC 5 cut(s) 156, 417, 438, 819, 976
HapII CCGG 1 cut(s) 186
HgaI GACGC 3 cut(s) 253, 568, 1330
HhaI GCGC 1 cut(s) 191
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 6 cut(s) 41, 331, 443, 602, 1162, 1416
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HincII GTYRAC 2 cut(s) 268, 651
HindII GTYRAC 2 cut(s) 268, 651
HindIII AAGCTT 2 cut(s) 321, 1218
HinfI GANTC 3 cut(s) 495, 884, 1225
HpaII CCGG 1 cut(s) 186
HphI GGTGA 3 cut(s) 110, 139, 970
Hpy166II GTNNAC 7 cut(s) 118, 268, 651, 833, 1019, 1045, 1117
Hpy188I TCNGA 1 cut(s) 1366
Hpy188III TCNNGA 2 cut(s) 485, 1283
Hpy8I GTNNAC 7 cut(s) 118, 268, 651, 833, 1019, 1045, 1117
Hpy99I CGWCG 3 cut(s) 242, 245, 269
HpyAV CCTTC 3 cut(s) 485, 558, 1348
HpyCH4III ACNGT 3 cut(s) 536, 1051, 1328
HpyCH4IV ACGT 2 cut(s) 240, 282
HpyF3I CTNAG 2 cut(s) 751, 1377
HpySE526I ACGT 2 cut(s) 240, 282
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 6 cut(s) 41, 331, 443, 602, 1162, 1416
HspAI GCGC 1 cut(s) 189
KspI CCGCGG 1 cut(s) 811
Kzo9I GATC 2 cut(s) 583, 696
LmnI GCTCC 5 cut(s) 196, 206, 550, 631, 848
Lsp1109I GCAGC 6 cut(s) 391, 410, 413, 934, 1063, 1135
LweI GCATC 6 cut(s) 171, 295, 517, 868, 1017, 1046
MaeI CTAG 2 cut(s) 81, 1397
MaeII ACGT 2 cut(s) 240, 282
MaeIII GTNAC 2 cut(s) 145, 1322
MalI GATC 2 cut(s) 585, 698
MboI GATC 2 cut(s) 583, 696
MboII GAAGA 3 cut(s) 58, 473, 851
MfeI CAATTG 2 cut(s) 432, 1128
MflI RGATCY 1 cut(s) 696
MhlI GDGCHC 4 cut(s) 547, 732, 853, 1021
MlsI TGGCCA 1 cut(s) 438
MluNI TGGCCA 1 cut(s) 438
MlyI GAGTC 1 cut(s) 893
MmeI TCCRAC 2 cut(s) 757, 1033
Mox20I TGGCCA 1 cut(s) 438
MroXI GAANNNNTTC 1 cut(s) 1371
MscI TGGCCA 1 cut(s) 438
MseI TTAA 8 cut(s) 18, 51, 228, 855, 900, 966, 1230, 1314
Msp20I TGGCCA 1 cut(s) 438
MspA1I CMGCKG 3 cut(s) 404, 755, 810
MspCI CTTAAG 1 cut(s) 50
MspI CCGG 1 cut(s) 186
MspR9I CCNGG 3 cut(s) 186, 409, 801
MunI CAATTG 2 cut(s) 432, 1128
Mva1269I GAATGC 2 cut(s) 638, 1025
MvaI CCWGG 2 cut(s) 409, 801
MvnI CGCG 1 cut(s) 810
NciI CCSGG 1 cut(s) 186
NcoI CCATGG 1 cut(s) 1158
NdeI CATATG 1 cut(s) 1087
NdeII GATC 2 cut(s) 583, 696
NlaIII CATG 6 cut(s) 41, 331, 443, 602, 1162, 1416
NlaIV GGNNCC 4 cut(s) 202, 633, 805, 975
NmeAIII GCCGAG 3 cut(s) 138, 231, 907
NmuCI GTSAC 2 cut(s) 145, 1322
NspV TTCGAA 1 cut(s) 1223
PceI AGGCCT 1 cut(s) 156
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 279, 765
PctI GAATGC 2 cut(s) 638, 1025
PdmI GAANNNNTTC 1 cut(s) 1371
PfeI GAWTC 2 cut(s) 495, 1225
PflFI GACNNNGTC 1 cut(s) 272
PleI GAGTC 1 cut(s) 892
PpsI GAGTC 1 cut(s) 892
PshBI ATTAAT 1 cut(s) 1314
Psp124BI GAGCTC 1 cut(s) 732
Psp6I CCWGG 2 cut(s) 407, 799
PspFI CCCAGC 1 cut(s) 570
PspGI CCWGG 2 cut(s) 407, 799
PspN4I GGNNCC 4 cut(s) 202, 633, 805, 975
PspPI GGNCC 5 cut(s) 331, 415, 803, 817, 974
PsrI GAACNNNNNNTAC 2 cut(s) 449, 481
PstI CTGCAG 1 cut(s) 1393
PsuI RGATCY 1 cut(s) 696
PsyI GACNNNGTC 1 cut(s) 272
PvuII CAGCTG 2 cut(s) 404, 755
RsaI GTAC 4 cut(s) 42, 666, 832, 1203
RsaNI GTAC 4 cut(s) 41, 665, 831, 1202
SacI GAGCTC 1 cut(s) 732
SacII CCGCGG 1 cut(s) 811
SalI GTCGAC 1 cut(s) 266
SaqAI TTAA 8 cut(s) 18, 51, 228, 855, 900, 966, 1230, 1314
Sau3AI GATC 2 cut(s) 583, 696
Sau96I GGNCC 5 cut(s) 331, 415, 803, 817, 974
ScaI AGTACT 1 cut(s) 1203
SchI GAGTC 1 cut(s) 893
ScrFI CCNGG 3 cut(s) 186, 409, 801
SduI GDGCHC 4 cut(s) 547, 732, 853, 1021
SfaNI GCATC 6 cut(s) 171, 295, 517, 868, 1017, 1046
SfcI CTRYAG 2 cut(s) 396, 1389
Sfr303I CCGCGG 1 cut(s) 811
SfuI TTCGAA 1 cut(s) 1223
SgrBI CCGCGG 1 cut(s) 811
SinI GGWCC 2 cut(s) 331, 803
SmlI CTYRAG 2 cut(s) 50, 932
SmoI CTYRAG 2 cut(s) 50, 932
SseBI AGGCCT 1 cut(s) 156
SspI AATATT 2 cut(s) 1105, 1260
SspMI CTAG 2 cut(s) 81, 1397
SstI GAGCTC 1 cut(s) 732
StuI AGGCCT 1 cut(s) 156
StyD4I CCNGG 3 cut(s) 184, 407, 799
StyI CCWWGG 1 cut(s) 1158
TaaI ACNGT 3 cut(s) 536, 1051, 1328
TaiI ACGT 2 cut(s) 243, 285
TaqI TCGA 6 cut(s) 237, 267, 613, 759, 795, 1223
TatI WGTACW 1 cut(s) 1201
TauI GCSGC 4 cut(s) 298, 301, 318, 352
TfiI GAWTC 2 cut(s) 495, 1225
Tru1I TTAA 8 cut(s) 18, 51, 228, 855, 900, 966, 1230, 1314
Tru9I TTAA 8 cut(s) 18, 51, 228, 855, 900, 966, 1230, 1314
TscAI CASTG 3 cut(s) 1054, 1146, 1333
TseFI GTSAC 2 cut(s) 145, 1322
TseI GCWGC 6 cut(s) 401, 404, 423, 922, 1076, 1148
Tsp45I GTSAC 2 cut(s) 145, 1322
TspDTI ATGAA 6 cut(s) 465, 504, 527, 1084, 1269, 1281
TspGWI ACGGA 3 cut(s) 133, 157, 652
TspRI CASTG 3 cut(s) 1054, 1146, 1333
Tth111I GACNNNGTC 1 cut(s) 272
Vha464I CTTAAG 1 cut(s) 50
VneI GTGCAC 1 cut(s) 1017
VpaK11BI GGWCC 2 cut(s) 331, 803
VspI ATTAAT 1 cut(s) 1314
XapI RAATTY 3 cut(s) 1244, 1270, 1287
XmiI GTMKAC 1 cut(s) 267
XmnI GAANNNNTTC 1 cut(s) 1371
XspI CTAG 2 cut(s) 81, 1397
ZraI GACGTC 1 cut(s) 241
ZrmI AGTACT 1 cut(s) 1203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.