Rorug06G0201400

pfkB family carbohydrate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0009506
Physical Location & Seq
Forward (+)
32330332 .. 32331129
798 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0201400.1

Sequence Viewer

Length: 798 bp
ATGGAACCACGAACTCTGCAACTAATGGAGCTACCAACCCAGCAACAAATCAAGGATGTAGGAAACAACCAAGCAGCCGGGAGTAGTGCCAACACGGATTTCTGGCGAAGCCGTACCAGGTGGATTCCTACTCCAGATCAAATAAGAATCCTCAAGGATCTTTACTACGACAAGGGAGTTAAGTGCCCAACTACAGAGCACATTCACGAGATCTGTCTCCAGCTGAACCAGTATGGACAGGTTGAAGGCAAGAACATTTATTTTTGGTTCCAGAACGTCAGGGCTCGAGAAAGGCAGATGAAGAGGTGTAATCAGGCTGCTCAAGTGCCCATGGGAACTAGTTCTCCTGGTACTGGTGGATCCATTAATCTCAATTTTGGGTCCACTGGTTCTACTGGTGCTGGTGGATCCATTGATCTCAATTTTGGGCCAGCTGGTGGATCCATTGACATCAATTTTGGGTCCACTAGTTCTACTGGTGCTGGTGGATCCATTGATCTCTATTTTGGGCCAGGCAGTGTTGACCCGTATTTCTCCTCACCCTTCACTACTAGTACTAGTACTACTTTCTTTGGCAGGAATGAAGAACAAACTTCCATGGAACAACGAGGAGAAGATCACCAGGAGATTGAAACCCTTCCACTGTTCCCCATGCACGATAAGGACATTGGCAACAAGAAGACTCCTTCCGAGGGAGGTAGCAGCTATGATGACTACTCAGGTGGCGGCTACAACGGTGGCTCTCGCATTGTTCTTGAGCTCAGCCTCAACTCCTCCGGATCTGCTGACATGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

265

Amino Acids

28.73

Weight (kDa)

5.03

Isoelectric Point (pI)

45.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 38 - 99 3.3e-09 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 437
AccIII TCCGGA 1 cut(s) 776
AciI CCGC 1 cut(s) 726
AfaI GTAC 4 cut(s) 115, 352, 556, 562
AfiI CCNNNNNNNGG 3 cut(s) 353, 437, 692
AgsI TTSAA 2 cut(s) 245, 632
AhlI ACTAGT 4 cut(s) 338, 467, 551, 557
AjnI CCWGG 4 cut(s) 116, 346, 511, 621
AjuI GAANNNNNNNTTGG 2 cut(s) 83, 115
AloI GAACNNNNNNTCC 2 cut(s) 328, 360
AluBI AGCT 5 cut(s) 31, 223, 434, 705, 760
AluI AGCT 5 cut(s) 31, 223, 434, 705, 760
Alw21I GWGCWC 2 cut(s) 201, 762
Alw26I GTCTC 1 cut(s) 221
Ama87I CYCGRG 1 cut(s) 285
Aor13HI TCCGGA 1 cut(s) 776
AoxI GGCC 2 cut(s) 428, 509
ApeKI GCWGC 3 cut(s) 74, 317, 702
ArsI GACNNNNNNTTYG 2 cut(s) 440, 472
AseI ATTAAT 1 cut(s) 366
Asp700I GAANNNNTTC 2 cut(s) 340, 636
AspS9I GGNCC 4 cut(s) 381, 428, 462, 509
AsuC2I CCSGG 1 cut(s) 79
AsuHPI GGTGA 2 cut(s) 531, 611
AvaI CYCGRG 1 cut(s) 285
AvaII GGWCC 2 cut(s) 381, 462
BaeGI GKGCMC 2 cut(s) 188, 330
BamHI GGATCC 4 cut(s) 359, 407, 440, 488
BanII GRGCYC 2 cut(s) 286, 762
BauI CACGAG 1 cut(s) 206
BbsI GAAGAC 1 cut(s) 686
Bbv12I GWGCWC 2 cut(s) 201, 762
BbvI GCAGC 3 cut(s) 86, 304, 714
BceAI ACGGC 1 cut(s) 96
BciT130I CCWGG 4 cut(s) 118, 348, 513, 623
BcnI CCSGG 1 cut(s) 79
BcoDI GTCTC 1 cut(s) 221
BcuI ACTAGT 4 cut(s) 338, 467, 551, 557
BfaI CTAG 4 cut(s) 339, 468, 552, 558
BfmI CTRYAG 1 cut(s) 192
BglII AGATCT 1 cut(s) 210
BisI GCNGC 4 cut(s) 75, 318, 703, 727
BlpI GCTNAGC 1 cut(s) 761
BlsI GCNGC 4 cut(s) 76, 319, 704, 728
BmcAI AGTACT 2 cut(s) 556, 562
Bme1390I CCNGG 5 cut(s) 79, 118, 348, 513, 623
Bme18I GGWCC 2 cut(s) 381, 462
BmeT110I CYCGRG 1 cut(s) 285
BmgT120I GGNCC 4 cut(s) 381, 428, 462, 509
BmiI GGNNCC 8 cut(s) 6, 269, 361, 382, 409, 442, 463, 490
BmrFI CCNGG 5 cut(s) 79, 118, 348, 513, 623
BpiI GAAGAC 1 cut(s) 686
BpmI CTGGAG 2 cut(s) 117, 203
Bpu1102I GCTNAGC 1 cut(s) 761
BpuEI CTTGAG 3 cut(s) 137, 306, 776
BpuMI CCSGG 1 cut(s) 79
BsaJI CCNNGG 3 cut(s) 330, 597, 690
BsaWI WCCGGW 1 cut(s) 776
BsaXI ACNNNNNCTCC 2 cut(s) 328, 358
Bsc4I CCNNNNNNNGG 3 cut(s) 353, 437, 692
Bse1I ACTGG 5 cut(s) 229, 358, 391, 400, 481
BseAI TCCGGA 1 cut(s) 776
BseBI CCWGG 4 cut(s) 118, 348, 513, 623
BseDI CCNNGG 3 cut(s) 330, 597, 690
BseGI GGATG 1 cut(s) 61
BseLI CCNNNNNNNGG 3 cut(s) 353, 437, 692
BseMII CTCAG 2 cut(s) 732, 775
BseNI ACTGG 5 cut(s) 229, 358, 391, 400, 481
BseRI GAGGAG 3 cut(s) 526, 624, 763
BseSI GKGCMC 2 cut(s) 188, 330
BseXI GCAGC 3 cut(s) 86, 304, 714
BseYI CCCAGC 1 cut(s) 39
BshFI GGCC 2 cut(s) 430, 511
BsiHKAI GWGCWC 2 cut(s) 201, 762
BsiHKCI CYCGRG 1 cut(s) 285
BsiSI CCGG 2 cut(s) 78, 777
BslI CCNNNNNNNGG 3 cut(s) 353, 437, 692
BsmAI GTCTC 1 cut(s) 221
BsnI GGCC 2 cut(s) 430, 511
BsoBI CYCGRG 1 cut(s) 285
Bsp1286I GDGCHC 5 cut(s) 188, 201, 286, 330, 762
Bsp13I TCCGGA 1 cut(s) 776
Bsp1720I GCTNAGC 1 cut(s) 761
Bsp19I CCATGG 2 cut(s) 330, 597
BspACI CCGC 1 cut(s) 726
BspANI GGCC 2 cut(s) 430, 511
BspCNI CTCAG 2 cut(s) 731, 774
BspEI TCCGGA 1 cut(s) 776
BspLI GGNNCC 8 cut(s) 6, 269, 361, 382, 409, 442, 463, 490
BsrI ACTGG 5 cut(s) 229, 358, 391, 400, 481
BssECI CCNNGG 3 cut(s) 330, 597, 690
BssSI CACGAG 1 cut(s) 206
BssT1I CCWWGG 2 cut(s) 330, 597
Bst2BI CACGAG 1 cut(s) 206
Bst2UI CCWGG 4 cut(s) 118, 348, 513, 623
Bst4CI ACNGT 2 cut(s) 645, 737
Bst6I CTCTTC 1 cut(s) 296
BstC8I GCNNGC 1 cut(s) 432
BstDEI CTNAG 3 cut(s) 718, 761, 795
BstDSI CCRYGG 2 cut(s) 330, 597
BstF5I GGATG 1 cut(s) 61
BstMAI GTCTC 1 cut(s) 221
BstMWI GCNNNNNNNGC 1 cut(s) 791
BstNI CCWGG 4 cut(s) 118, 348, 513, 623
BstSCI CCNGG 5 cut(s) 77, 116, 346, 511, 621
BstSFI CTRYAG 1 cut(s) 192
BstSLI GKGCMC 2 cut(s) 188, 330
BstV1I GCAGC 3 cut(s) 86, 304, 714
BstV2I GAAGAC 1 cut(s) 686
BstX2I RGATCY 7 cut(s) 157, 210, 359, 407, 440, 488, 779
BstYI RGATCY 7 cut(s) 157, 210, 359, 407, 440, 488, 779
BsuRI GGCC 2 cut(s) 430, 511
BtgI CCRYGG 2 cut(s) 330, 597
BtsCI GGATG 1 cut(s) 61
BtsI GCAGTG 1 cut(s) 523
BtsIMutI CAGTG 3 cut(s) 384, 523, 641
Cac8I GCNNGC 1 cut(s) 432
Cfr13I GGNCC 4 cut(s) 381, 428, 462, 509
CsiI ACCWGGT 1 cut(s) 116
Csp6I GTAC 4 cut(s) 114, 351, 555, 561
CviAII CATG 4 cut(s) 331, 598, 652, 790
CviQI GTAC 4 cut(s) 114, 351, 555, 561
DdeI CTNAG 3 cut(s) 718, 761, 795
Eam1104I CTCTTC 1 cut(s) 296
EarI CTCTTC 1 cut(s) 296
Ecl136II GAGCTC 1 cut(s) 760
Eco130I CCWWGG 2 cut(s) 330, 597
Eco24I GRGCYC 2 cut(s) 286, 762
Eco47I GGWCC 2 cut(s) 381, 462
Eco53kI GAGCTC 1 cut(s) 760
Eco88I CYCGRG 1 cut(s) 285
EcoICRI GAGCTC 1 cut(s) 760
EcoRII CCWGG 4 cut(s) 116, 346, 511, 621
EcoT14I CCWWGG 2 cut(s) 330, 597
EcoT38I GRGCYC 2 cut(s) 286, 762
ErhI CCWWGG 2 cut(s) 330, 597
FaeI CATG 4 cut(s) 334, 601, 655, 793
FaiI YATR 6 cut(s) 234, 332, 599, 653, 708, 791
FatI CATG 4 cut(s) 330, 597, 651, 789
Fnu4HI GCNGC 4 cut(s) 75, 318, 703, 727
FokI GGATG 1 cut(s) 68
FriOI GRGCYC 2 cut(s) 286, 762
Fsp4HI GCNGC 4 cut(s) 75, 318, 703, 727
FspBI CTAG 4 cut(s) 339, 468, 552, 558
GluI GCNGC 4 cut(s) 75, 318, 703, 727
GsaI CCCAGC 1 cut(s) 43
GsuI CTGGAG 2 cut(s) 117, 203
HaeIII GGCC 2 cut(s) 430, 511
HapII CCGG 2 cut(s) 78, 777
Hin1II CATG 4 cut(s) 334, 601, 655, 793
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HinfI GANTC 3 cut(s) 124, 147, 682
HpaII CCGG 2 cut(s) 78, 777
HphI GGTGA 2 cut(s) 531, 611
Hpy166II GTNNAC 3 cut(s) 384, 465, 523
Hpy188I TCNGA 1 cut(s) 691
Hpy188III TCNNGA 6 cut(s) 134, 206, 271, 287, 755, 777
Hpy8I GTNNAC 3 cut(s) 384, 465, 523
HpyAV CCTTC 4 cut(s) 239, 553, 647, 696
HpyCH4III ACNGT 2 cut(s) 645, 737
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 2 cut(s) 19, 655
HpyF10VI GCNNNNNNNGC 1 cut(s) 791
HpyF3I CTNAG 3 cut(s) 718, 761, 795
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 4 cut(s) 334, 601, 655, 793
Kpn2I TCCGGA 1 cut(s) 776
LmnI GCTCC 1 cut(s) 28
Lsp1109I GCAGC 3 cut(s) 86, 304, 714
MabI ACCWGGT 1 cut(s) 116
MaeI CTAG 4 cut(s) 339, 468, 552, 558
MaeII ACGT 1 cut(s) 276
MboII GAAGA 4 cut(s) 313, 596, 626, 691
MflI RGATCY 7 cut(s) 157, 210, 359, 407, 440, 488, 779
MhlI GDGCHC 5 cut(s) 188, 201, 286, 330, 762
MluCI AATT 3 cut(s) 373, 421, 454
MlyI GAGTC 1 cut(s) 676
MnlI CCTC 8 cut(s) 161, 297, 547, 602, 685, 689, 776, 784
MroI TCCGGA 1 cut(s) 776
MroXI GAANNNNTTC 2 cut(s) 340, 636
MseI TTAA 2 cut(s) 180, 366
MspA1I CMGCKG 2 cut(s) 223, 434
MspI CCGG 2 cut(s) 78, 777
MspR9I CCNGG 5 cut(s) 79, 118, 348, 513, 623
MvaI CCWGG 4 cut(s) 118, 348, 513, 623
MwoI GCNNNNNNNGC 1 cut(s) 791
NciI CCSGG 1 cut(s) 79
NcoI CCATGG 2 cut(s) 330, 597
NlaIII CATG 4 cut(s) 334, 601, 655, 793
NlaIV GGNNCC 8 cut(s) 6, 269, 361, 382, 409, 442, 463, 490
PaeR7I CTCGAG 1 cut(s) 285
PdmI GAANNNNTTC 2 cut(s) 340, 636
PfeI GAWTC 2 cut(s) 124, 147
PflMI CCANNNNNTGG 1 cut(s) 437
PkrI GCNGC 4 cut(s) 76, 319, 704, 728
PleI GAGTC 1 cut(s) 676
PpsI GAGTC 1 cut(s) 676
PshBI ATTAAT 1 cut(s) 366
Psp124BI GAGCTC 1 cut(s) 762
Psp6I CCWGG 4 cut(s) 116, 346, 511, 621
PspFI CCCAGC 1 cut(s) 39
PspGI CCWGG 4 cut(s) 116, 346, 511, 621
PspN4I GGNNCC 8 cut(s) 6, 269, 361, 382, 409, 442, 463, 490
PspPI GGNCC 4 cut(s) 381, 428, 462, 509
PsuI RGATCY 7 cut(s) 157, 210, 359, 407, 440, 488, 779
PvuII CAGCTG 2 cut(s) 223, 434
RsaI GTAC 4 cut(s) 115, 352, 556, 562
RsaNI GTAC 4 cut(s) 114, 351, 555, 561
SacI GAGCTC 1 cut(s) 762
SaqAI TTAA 2 cut(s) 180, 366
SatI GCNGC 4 cut(s) 75, 318, 703, 727
Sau96I GGNCC 4 cut(s) 381, 428, 462, 509
ScaI AGTACT 2 cut(s) 556, 562
SchI GAGTC 1 cut(s) 676
ScrFI CCNGG 5 cut(s) 79, 118, 348, 513, 623
SduI GDGCHC 5 cut(s) 188, 201, 286, 330, 762
SexAI ACCWGGT 1 cut(s) 116
SfcI CTRYAG 1 cut(s) 192
Sfr274I CTCGAG 1 cut(s) 285
SinI GGWCC 2 cut(s) 381, 462
SlaI CTCGAG 1 cut(s) 285
SmlI CTYRAG 4 cut(s) 152, 285, 321, 755
SmoI CTYRAG 4 cut(s) 152, 285, 321, 755
SpeI ACTAGT 4 cut(s) 338, 467, 551, 557
Sse9I AATT 3 cut(s) 373, 421, 454
SsiI CCGC 1 cut(s) 726
SspMI CTAG 4 cut(s) 339, 468, 552, 558
SstI GAGCTC 1 cut(s) 762
StyD4I CCNGG 5 cut(s) 77, 116, 346, 511, 621
StyI CCWWGG 2 cut(s) 330, 597
TaaI ACNGT 2 cut(s) 645, 737
TaiI ACGT 1 cut(s) 279
TaqI TCGA 1 cut(s) 286
TasI AATT 3 cut(s) 373, 421, 454
TatI WGTACW 2 cut(s) 554, 560
TauI GCSGC 1 cut(s) 729
TfiI GAWTC 2 cut(s) 124, 147
Tru1I TTAA 2 cut(s) 180, 366
Tru9I TTAA 2 cut(s) 180, 366
TscAI CASTG 3 cut(s) 391, 523, 648
TseI GCWGC 3 cut(s) 74, 317, 702
TspDTI ATGAA 2 cut(s) 314, 597
TspGWI ACGGA 1 cut(s) 110
TspRI CASTG 3 cut(s) 391, 523, 648
Van91I CCANNNNNTGG 1 cut(s) 437
VpaK11BI GGWCC 2 cut(s) 381, 462
VspI ATTAAT 1 cut(s) 366
XhoI CTCGAG 1 cut(s) 285
XmnI GAANNNNTTC 2 cut(s) 340, 636
XspI CTAG 4 cut(s) 339, 468, 552, 558
ZrmI AGTACT 2 cut(s) 556, 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.