Rroxscaffold_7G00179520

pfkB family carbohydrate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009506
Physical Location & Seq
Reverse (-)
19060812 .. 19064416
3605 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00179520.1

Sequence Viewer

Length: 1053 bp
ATGGCTATAGCAGCTGCCAGGCTGGGGCTTCGCTGCAATGCAATTGGCCATGTGGGTAATGAAGTGTATGGGCGGTTCTTATTAGATGTTCTTCGTGATGAAGGAATCAGTATGGTTGGGATGAATGAGGATACTGATGCTGACAGTTCAAGTGCTTCTTCGTATGAGACGCTTTTGTGCTGGGTTCTTGTGGATCAGTTGCAAAGACATGGTTTTTGTAGTCGAGCTGATTTCAGTAAGGAGCCTGCATTCAATTGGTTGACCAAATTATCCGTGCAAGTAAAGACGGCGATAAAACAGTCAAAGATCCTGTTCTGTAATGGTTATGGCTTTGACGAGCTCTCTCCTGCTGTAATAGCCTCAGCTGTAGAGTATGCTGTTGAAGTTGGAACAGCAGTTTTTTTCGACCCTGGACCCCGCGGAAAGAGCCTCTCGGCTGGTACACCTGAAGAACAAGGAGCACTTAACCTGTTGTTGAGGATGAGTGACGTTCTTCTTCTAACTTCAGATGAGGCTGAGTCATTGACTGGCATTAAAAACCCGATATTAGCAGGGCAGCAGTTGCTCAAGCAAGGGGTGCAAACAAAATGGGTGATTGTTAAAATGGGGCCAAAGGGTTCAATTCTCATAACCAAGTCTGGCATCTCTTGTGCACCAGCATTCAAGGTAAAGGTCATGGACACTGTTGGATGCGGAGATAGTTTTGTAGCTGCCATTGCATATGGTTTCATCCACAATATGCCAATGGTAAATACATTAGCAATTGCAAATGCAGTGGGTGCTGCAACTGCCATGGGTTGTGGTGCTGGTAGGAATGTGGCGACATTGGATAAAGTACTTGAAATTGTGAAAGCTTCGAATCTTAATGAGGATGACGAATTTTGGAATGAAATATTTGATGAAAATTTGGGTGTTCAGGAAATTACTGCTCTCTCAATATTGGTCATTAATGGAAGTGACAGTCAGCTGAATAGCGTCCCTTTGCAGAAGGTTGTATCTGAATTGCTTCCTAAGCTCAAATCTGCAGAGCTAGAGAGAAAAGTGCCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

350

Amino Acids

37.41

Weight (kDa)

5.01

Isoelectric Point (pI)

29.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 1 - 269 1.1e-33 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 420
AciI CCGC 4 cut(s) 73, 418, 420, 693
AclWI GGATC 2 cut(s) 201, 301
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 2 cut(s) 878, 904
AcuI CTGAAG 2 cut(s) 468, 489
AfaI GTAC 2 cut(s) 442, 837
AfiI CCNNNNNNNGG 1 cut(s) 24
AgsI TTSAA 6 cut(s) 150, 253, 383, 621, 664, 842
AjnI CCWGG 2 cut(s) 17, 409
AluBI AGCT 9 cut(s) 14, 227, 340, 365, 710, 854, 967, 1015, 1030
AluI AGCT 9 cut(s) 14, 227, 340, 365, 710, 854, 967, 1015, 1030
Alw21I GWGCWC 3 cut(s) 342, 463, 655
Alw26I GTCTC 1 cut(s) 161
Alw44I GTGCAC 1 cut(s) 651
AlwI GGATC 2 cut(s) 201, 301
AoxI GGCC 2 cut(s) 46, 608
ApaLI GTGCAC 1 cut(s) 651
ApeKI GCWGC 6 cut(s) 11, 14, 33, 556, 710, 782
ApoI RAATTY 2 cut(s) 878, 904
ArsI GACNNNNNNTTYG 2 cut(s) 198, 230
AseI ATTAAT 1 cut(s) 948
Asp700I GAANNNNTTC 1 cut(s) 1005
AspS9I GGNCC 2 cut(s) 413, 608
AsuHPI GGTGA 1 cut(s) 604
AsuII TTCGAA 1 cut(s) 857
AvaII GGWCC 1 cut(s) 413
BaeGI GKGCMC 1 cut(s) 655
BalI TGGCCA 1 cut(s) 48
BanII GRGCYC 1 cut(s) 342
Bbv12I GWGCWC 3 cut(s) 342, 463, 655
BbvCI CCTCAGC 1 cut(s) 361
BbvI GCAGC 5 cut(s) 20, 23, 568, 697, 769
BceAI ACGGC 1 cut(s) 303
BciT130I CCWGG 2 cut(s) 19, 411
BciVI GTATCC 1 cut(s) 124
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 1 cut(s) 1031
BfmI CTRYAG 3 cut(s) 6, 366, 1023
BfuI GTATCC 1 cut(s) 124
BisI GCNGC 6 cut(s) 12, 15, 34, 557, 711, 783
BlsI GCNGC 6 cut(s) 13, 16, 35, 558, 712, 784
BmcAI AGTACT 1 cut(s) 837
Bme1390I CCNGG 2 cut(s) 19, 411
Bme18I GGWCC 1 cut(s) 413
BmgT120I GGNCC 2 cut(s) 413, 608
BmiI GGNNCC 3 cut(s) 243, 415, 609
BmrFI CCNGG 2 cut(s) 19, 411
BmsI GCATC 3 cut(s) 127, 651, 680
Bpu10I CCTNAGC 2 cut(s) 361, 1011
Bpu14I TTCGAA 1 cut(s) 857
BpuEI CTTGAG 1 cut(s) 551
BsaJI CCNNGG 3 cut(s) 409, 418, 792
Bsc4I CCNNNNNNNGG 1 cut(s) 24
Bse1I ACTGG 1 cut(s) 532
Bse3DI GCAATG 2 cut(s) 43, 714
BseBI CCWGG 2 cut(s) 19, 411
BseDI CCNNGG 3 cut(s) 409, 418, 792
BseGI GGATG 5 cut(s) 126, 486, 695, 729, 877
BseLI CCNNNNNNNGG 1 cut(s) 24
BseMI GCAATG 2 cut(s) 43, 714
BseMII CTCAG 2 cut(s) 375, 507
BseNI ACTGG 1 cut(s) 532
BseSI GKGCMC 1 cut(s) 655
BseXI GCAGC 5 cut(s) 20, 23, 568, 697, 769
BseYI CCCAGC 2 cut(s) 22, 180
Bsh1236I CGCG 1 cut(s) 420
BshFI GGCC 2 cut(s) 48, 610
BsiHKAI GWGCWC 3 cut(s) 342, 463, 655
BslFI GGGAC 1 cut(s) 962
BslI CCNNNNNNNGG 1 cut(s) 24
BsmAI GTCTC 1 cut(s) 161
BsmBI CGTCTC 1 cut(s) 161
BsmFI GGGAC 1 cut(s) 962
BsmI GAATGC 2 cut(s) 248, 659
BsnI GGCC 2 cut(s) 48, 610
Bsp119I TTCGAA 1 cut(s) 857
Bsp1286I GDGCHC 3 cut(s) 342, 463, 655
Bsp143I GATC 2 cut(s) 193, 306
Bsp19I CCATGG 1 cut(s) 792
BspACI CCGC 4 cut(s) 73, 418, 420, 693
BspANI GGCC 2 cut(s) 48, 610
BspCNI CTCAG 2 cut(s) 374, 508
BspFNI CGCG 1 cut(s) 420
BspLI GGNNCC 3 cut(s) 243, 415, 609
BspMAI CTGCAG 1 cut(s) 1027
BspPI GGATC 2 cut(s) 201, 301
BspT104I TTCGAA 1 cut(s) 857
BsrDI GCAATG 2 cut(s) 43, 714
BsrI ACTGG 1 cut(s) 532
BssECI CCNNGG 3 cut(s) 409, 418, 792
BssMI GATC 2 cut(s) 193, 306
BssT1I CCWWGG 1 cut(s) 792
Bst2UI CCWGG 2 cut(s) 19, 411
Bst4CI ACNGT 4 cut(s) 146, 300, 685, 962
BstAPI GCANNNNNTGC 3 cut(s) 562, 577, 779
BstBI TTCGAA 1 cut(s) 857
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 3 cut(s) 361, 516, 1011
BstDSI CCRYGG 2 cut(s) 418, 792
BstF5I GGATG 5 cut(s) 126, 486, 695, 729, 877
BstFNI CGCG 1 cut(s) 420
BstKTI GATC 2 cut(s) 196, 309
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 2 cut(s) 193, 306
BstMWI GCNNNNNNNGC 9 cut(s) 11, 356, 426, 562, 577, 716, 779, 788, 1012
BstNI CCWGG 2 cut(s) 19, 411
BstSCI CCNGG 2 cut(s) 17, 409
BstSFI CTRYAG 3 cut(s) 6, 366, 1023
BstSLI GKGCMC 1 cut(s) 655
BstUI CGCG 1 cut(s) 420
BstV1I GCAGC 5 cut(s) 20, 23, 568, 697, 769
BstX2I RGATCY 1 cut(s) 306
BstYI RGATCY 1 cut(s) 306
BsuI GTATCC 1 cut(s) 124
BsuRI GGCC 2 cut(s) 48, 610
BtgI CCRYGG 2 cut(s) 418, 792
BtsCI GGATG 5 cut(s) 126, 486, 695, 729, 877
BtsI GCAGTG 1 cut(s) 780
BtsIMutI CAGTG 2 cut(s) 681, 780
Cac8I GCNNGC 1 cut(s) 246
Cfr13I GGNCC 2 cut(s) 413, 608
Cfr42I CCGCGG 1 cut(s) 421
CseI GACGC 2 cut(s) 178, 964
Csp6I GTAC 2 cut(s) 441, 836
CspCI CAANNNNNGTGG 2 cut(s) 756, 791
CviAII CATG 5 cut(s) 50, 209, 676, 793, 1047
CviQI GTAC 2 cut(s) 441, 836
DdeI CTNAG 3 cut(s) 361, 516, 1011
DpnI GATC 2 cut(s) 195, 308
DpnII GATC 2 cut(s) 193, 306
EaeI YGGCCR 1 cut(s) 46
Ecl136II GAGCTC 1 cut(s) 340
Eco130I CCWWGG 1 cut(s) 792
Eco24I GRGCYC 1 cut(s) 342
Eco47I GGWCC 1 cut(s) 413
Eco53kI GAGCTC 1 cut(s) 340
Eco57I CTGAAG 2 cut(s) 468, 489
EcoICRI GAGCTC 1 cut(s) 340
EcoRII CCWGG 2 cut(s) 17, 409
EcoT14I CCWWGG 1 cut(s) 792
EcoT38I GRGCYC 1 cut(s) 342
ErhI CCWWGG 1 cut(s) 792
Esp3I CGTCTC 1 cut(s) 161
FaeI CATG 5 cut(s) 53, 212, 679, 796, 1050
FalI AAGNNNNNCTT 4 cut(s) 142, 174, 447, 479
FaqI GGGAC 1 cut(s) 962
FatI CATG 5 cut(s) 49, 208, 675, 792, 1046
FauI CCCGC 1 cut(s) 425
FauNDI CATATG 1 cut(s) 721
Fnu4HI GCNGC 6 cut(s) 12, 15, 34, 557, 711, 783
FokI GGATG 5 cut(s) 133, 493, 702, 716, 884
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 6 cut(s) 12, 15, 34, 557, 711, 783
FspBI CTAG 1 cut(s) 1031
GluI GCNGC 6 cut(s) 12, 15, 34, 557, 711, 783
GsaI CCCAGC 2 cut(s) 26, 184
HaeIII GGCC 2 cut(s) 48, 610
HgaI GACGC 2 cut(s) 178, 964
Hin1II CATG 5 cut(s) 53, 212, 679, 796, 1050
HincII GTYRAC 1 cut(s) 261
HindII GTYRAC 1 cut(s) 261
HindIII AAGCTT 1 cut(s) 852
HinfI GANTC 3 cut(s) 105, 518, 859
HphI GGTGA 1 cut(s) 604
Hpy166II GTNNAC 3 cut(s) 261, 443, 653
Hpy188I TCNGA 2 cut(s) 508, 1000
Hpy188III TCNNGA 2 cut(s) 95, 917
Hpy8I GTNNAC 3 cut(s) 261, 443, 653
HpyAV CCTTC 2 cut(s) 95, 982
HpyCH4III ACNGT 4 cut(s) 146, 300, 685, 962
HpyCH4IV ACGT 1 cut(s) 489
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 356, 426, 562, 577, 716, 779, 788, 1012
HpyF3I CTNAG 3 cut(s) 361, 516, 1011
HpySE526I ACGT 1 cut(s) 489
Hsp92II CATG 5 cut(s) 53, 212, 679, 796, 1050
KspI CCGCGG 1 cut(s) 421
Kzo9I GATC 2 cut(s) 193, 306
LmnI GCTCC 2 cut(s) 241, 458
Lsp1109I GCAGC 5 cut(s) 20, 23, 568, 697, 769
LweI GCATC 3 cut(s) 127, 651, 680
MaeI CTAG 1 cut(s) 1031
MaeII ACGT 1 cut(s) 489
MaeIII GTNAC 2 cut(s) 485, 956
MalI GATC 2 cut(s) 195, 308
MboI GATC 2 cut(s) 193, 306
MboII GAAGA 5 cut(s) 83, 150, 461, 485, 488
MfeI CAATTG 3 cut(s) 42, 253, 762
MflI RGATCY 1 cut(s) 306
MhlI GDGCHC 3 cut(s) 342, 463, 655
MlsI TGGCCA 1 cut(s) 48
MluNI TGGCCA 1 cut(s) 48
MlyI GAGTC 1 cut(s) 527
MmeI TCCRAC 2 cut(s) 367, 667
MnlI CCTC 6 cut(s) 121, 370, 440, 471, 505, 862
Mox20I TGGCCA 1 cut(s) 48
MroXI GAANNNNTTC 1 cut(s) 1005
MscI TGGCCA 1 cut(s) 48
MseI TTAA 5 cut(s) 465, 534, 600, 864, 948
Msp20I TGGCCA 1 cut(s) 48
MspA1I CMGCKG 4 cut(s) 14, 365, 420, 967
MspR9I CCNGG 2 cut(s) 19, 411
MunI CAATTG 3 cut(s) 42, 253, 762
Mva1269I GAATGC 2 cut(s) 248, 659
MvaI CCWGG 2 cut(s) 19, 411
MvnI CGCG 1 cut(s) 420
MwoI GCNNNNNNNGC 9 cut(s) 11, 356, 426, 562, 577, 716, 779, 788, 1012
NcoI CCATGG 1 cut(s) 792
NdeI CATATG 1 cut(s) 721
NdeII GATC 2 cut(s) 193, 306
NlaIII CATG 5 cut(s) 53, 212, 679, 796, 1050
NlaIV GGNNCC 3 cut(s) 243, 415, 609
NmeAIII GCCGAG 1 cut(s) 413
NmuCI GTSAC 2 cut(s) 485, 956
NspV TTCGAA 1 cut(s) 857
PctI GAATGC 2 cut(s) 248, 659
PdmI GAANNNNTTC 1 cut(s) 1005
PfeI GAWTC 2 cut(s) 105, 859
PkrI GCNGC 6 cut(s) 13, 16, 35, 558, 712, 784
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
PshBI ATTAAT 1 cut(s) 948
Psp124BI GAGCTC 1 cut(s) 342
Psp6I CCWGG 2 cut(s) 17, 409
PspFI CCCAGC 2 cut(s) 22, 180
PspGI CCWGG 2 cut(s) 17, 409
PspN4I GGNNCC 3 cut(s) 243, 415, 609
PspPI GGNCC 2 cut(s) 413, 608
PsrI GAACNNNNNNTAC 2 cut(s) 59, 91
PstI CTGCAG 1 cut(s) 1027
PsuI RGATCY 1 cut(s) 306
PvuII CAGCTG 3 cut(s) 14, 365, 967
RsaI GTAC 2 cut(s) 442, 837
RsaNI GTAC 2 cut(s) 441, 836
SacI GAGCTC 1 cut(s) 342
SacII CCGCGG 1 cut(s) 421
SaqAI TTAA 5 cut(s) 465, 534, 600, 864, 948
SatI GCNGC 6 cut(s) 12, 15, 34, 557, 711, 783
Sau3AI GATC 2 cut(s) 193, 306
Sau96I GGNCC 2 cut(s) 413, 608
ScaI AGTACT 1 cut(s) 837
SchI GAGTC 1 cut(s) 527
ScrFI CCNGG 2 cut(s) 19, 411
SduI GDGCHC 3 cut(s) 342, 463, 655
SfaNI GCATC 3 cut(s) 127, 651, 680
SfcI CTRYAG 3 cut(s) 6, 366, 1023
Sfr303I CCGCGG 1 cut(s) 421
SfuI TTCGAA 1 cut(s) 857
SgrBI CCGCGG 1 cut(s) 421
SinI GGWCC 1 cut(s) 413
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
SsiI CCGC 4 cut(s) 73, 418, 420, 693
SspI AATATT 2 cut(s) 894, 939
SspMI CTAG 1 cut(s) 1031
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 2 cut(s) 17, 409
StyI CCWWGG 1 cut(s) 792
TaaI ACNGT 4 cut(s) 146, 300, 685, 962
TaiI ACGT 1 cut(s) 492
TaqI TCGA 3 cut(s) 223, 405, 857
TatI WGTACW 1 cut(s) 835
TfiI GAWTC 2 cut(s) 105, 859
Tru1I TTAA 5 cut(s) 465, 534, 600, 864, 948
Tru9I TTAA 5 cut(s) 465, 534, 600, 864, 948
TscAI CASTG 2 cut(s) 688, 780
TseFI GTSAC 2 cut(s) 485, 956
TseI GCWGC 6 cut(s) 11, 14, 33, 556, 710, 782
Tsp45I GTSAC 2 cut(s) 485, 956
TspDTI ATGAA 6 cut(s) 75, 114, 137, 718, 903, 915
TspGWI ACGGA 1 cut(s) 262
TspRI CASTG 2 cut(s) 688, 780
VneI GTGCAC 1 cut(s) 651
VpaK11BI GGWCC 1 cut(s) 413
VspI ATTAAT 1 cut(s) 948
XapI RAATTY 2 cut(s) 878, 904
XmnI GAANNNNTTC 1 cut(s) 1005
XspI CTAG 1 cut(s) 1031
ZrmI AGTACT 1 cut(s) 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.