Rh6AG312500

pfkB family carbohydrate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0009506
Physical Location & Seq
Forward (+)
51186022 .. 51189651
3630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG312500.1

Sequence Viewer

Length: 1443 bp
ATGCACACCATAACCCTCAAAACCCCAATCCCCTACCATGTACCCTCCCGTAAGTCCCACTCCGTCTTCCCCCAAAACCCTAGACTAACCCGACCGCACCTCATTTTCCCGCCAATTCACCACCGCCCCCACTCTCACTCCTCCGTCTCCAAAGGCCTCGGCATCTCCGTCCCCAACTCCACCCCCGGCGCTCTCAACGGCTCCCTCCACTCCGAAAACCTCGCCGTTAAAGACATCGACGTCGCAACTCTCGGCAATCTCTGCGTCGATATCGTCCTCAACGTCCCCCAGTTGCCGCCGCCCGATGCCGACCGCCGCAAAGCTTACATGGACCGCCTATCCGCCTCGCCGCCCGATAAGAAATACTGGGAAGCTGGTGGCAACTGCAATATGGCTATAGCAGCTGCCAGGTTGGGGCTTCGCTGCAATGCAATTGGCCATGTGGGTAATGAAGTGTATGGGCGGTTCTTATTAGATGTTCTTCGTGATGAAGGAATCGGTATGGTTGGGATGAATGAGGACACTGATGCTAACAGTTCAAGTGCTTCTTCGTATGAAACACTTTTGTGCTGGGTTCTTGTGGATCAGTTGCAAAGACATGGTTTTTGTAGTCGAGCTGATTTCAGTAAGGAGCCTGCATTCAGTTGGTTGACCAAATTATCCGTACAAGTAAAGACGGCGATAAAACAGTCAAAGATCCTGTTCTGTAATGGTTATGGCTTTGACGAGCTCTCCCCTGCTGTAATAGCCTCAGCTGTAGAGTATGCTGTTGAAGTTGGAACAGCAATTTTTTTCGACCCTGGTCCCCGCGGAAAGAGCCTCTCTGCTGGTACACCTGAAGAACAAGGAGCACTTAACCTGTTGTTGAGGATGAGTGACGTTCTTCTTCTAACTTCAGATGAGGCTGAGTCATTGACTGGCATTAAAAACCCGATATTAGCAGGGCAGCAGTTGCTCAAGCAAGGGGTGCAAACAAAATGGGTGATTGTTAAAATGGGGCCAAAGGGTTCAATTCTCATAACTAAGTCTGGCATCTCTTGTGCACCAGCATTCAAGGTAAAGGTCATGGACACTGTTGGATGCGGAGACAGTTTTGTAGCTGCCATTGCATATGGTTTCATCCACAATATGCCAATGGTAAATACATTAGCAATTGCAAATGCAGTGGGTGCTGCAACTGCCATGGGTTGTGGTGCTGGTAGGAATGTGGCGACGTTGGATAAAGTACTTGAAATTGTGAAAGCTTCAAATCTTAATGAGGATGACGAATTTTGGAATGAAATATTTGATGAAAATTTGGGTGTTCAGGAAATTACAGCTGTCTCAAAATTGGTCAGTAATGGAAGTGACAGTCAGCTGAATAGTGTCCCTTTGCAGAAGGTTGTATCTGAATTGCTTCCTAAGCTCAAATCTGCAGAGCTAGAGAGAAAAGTGCCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

480

Amino Acids

51.56

Weight (kDa)

6.64

Isoelectric Point (pI)

32.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 122 - 399 2.6e-36 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 239
AatII GACGTC 1 cut(s) 243
AccII CGCG 1 cut(s) 810
AclWI GGATC 2 cut(s) 591, 691
AcoI YGGCCR 1 cut(s) 436
AcsI RAATTY 2 cut(s) 1268, 1294
AcuI CTGAAG 2 cut(s) 858, 879
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 4 cut(s) 42, 666, 832, 1227
AfiI CCNNNNNNNGG 1 cut(s) 414
AgsI TTSAA 6 cut(s) 540, 773, 1011, 1054, 1232, 1248
AjnI CCWGG 2 cut(s) 407, 799
AleI CACNNNNGTG 1 cut(s) 565
Alw21I GWGCWC 3 cut(s) 732, 853, 1045
Alw26I GTCTC 3 cut(s) 151, 1080, 1327
Alw44I GTGCAC 1 cut(s) 1041
AlwI GGATC 2 cut(s) 591, 691
AoxI GGCC 3 cut(s) 154, 436, 998
ApaLI GTGCAC 1 cut(s) 1041
ApeKI GCWGC 6 cut(s) 401, 404, 423, 946, 1100, 1172
ApoI RAATTY 2 cut(s) 1268, 1294
ArsI GACNNNNNNTTYG 2 cut(s) 588, 620
Asp700I GAANNNNTTC 1 cut(s) 1395
AspLEI GCGC 1 cut(s) 191
AspS9I GGNCC 3 cut(s) 331, 803, 998
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 2 cut(s) 110, 994
AvaII GGWCC 2 cut(s) 331, 803
BaeGI GKGCMC 1 cut(s) 1045
BalI TGGCCA 1 cut(s) 438
BanII GRGCYC 1 cut(s) 732
BbsI GAAGAC 1 cut(s) 58
Bbv12I GWGCWC 3 cut(s) 732, 853, 1045
BbvCI CCTCAGC 1 cut(s) 751
BbvI GCAGC 6 cut(s) 391, 410, 413, 958, 1087, 1159
BceAI ACGGC 3 cut(s) 209, 214, 693
BciT130I CCWGG 2 cut(s) 409, 801
BcnI CCSGG 1 cut(s) 186
BcoDI GTCTC 3 cut(s) 151, 1080, 1327
BfaI CTAG 2 cut(s) 81, 1421
BfmI CTRYAG 3 cut(s) 396, 756, 1413
BfoI RGCGCY 1 cut(s) 192
BmcAI AGTACT 1 cut(s) 1227
Bme1390I CCNGG 3 cut(s) 186, 409, 801
Bme18I GGWCC 2 cut(s) 331, 803
BmgT120I GGNCC 3 cut(s) 331, 803, 998
BmiI GGNNCC 4 cut(s) 202, 633, 805, 999
BmrFI CCNGG 3 cut(s) 186, 409, 801
BmrI ACTGGG 2 cut(s) 283, 376
BmsI GCATC 5 cut(s) 171, 295, 517, 1041, 1070
BmuI ACTGGG 2 cut(s) 283, 376
BoxI GACNNNNGTC 1 cut(s) 801
BpiI GAAGAC 1 cut(s) 58
Bpu10I CCTNAGC 2 cut(s) 751, 1401
BpuEI CTTGAG 1 cut(s) 941
BpuMI CCSGG 1 cut(s) 186
BsaHI GRCGYC 1 cut(s) 240
BsaJI CCNNGG 5 cut(s) 157, 184, 799, 808, 1182
BsaXI ACNNNNNCTCC 4 cut(s) 122, 152, 716, 746
Bsc4I CCNNNNNNNGG 1 cut(s) 414
Bse1I ACTGG 3 cut(s) 289, 371, 922
Bse3DI GCAATG 2 cut(s) 433, 1104
BseBI CCWGG 2 cut(s) 409, 801
BseDI CCNNGG 5 cut(s) 157, 184, 799, 808, 1182
BseGI GGATG 5 cut(s) 516, 876, 1085, 1119, 1267
BseLI CCNNNNNNNGG 1 cut(s) 414
BseMI GCAATG 2 cut(s) 433, 1104
BseMII CTCAG 2 cut(s) 765, 897
BseNI ACTGG 3 cut(s) 289, 371, 922
BseRI GAGGAG 1 cut(s) 130
BseSI GKGCMC 1 cut(s) 1045
BseXI GCAGC 6 cut(s) 391, 410, 413, 958, 1087, 1159
BseYI CCCAGC 1 cut(s) 570
Bsh1236I CGCG 1 cut(s) 810
Bsh1285I CGRYCG 2 cut(s) 95, 313
BshFI GGCC 3 cut(s) 156, 438, 1000
BsiEI CGRYCG 2 cut(s) 95, 313
BsiHKAI GWGCWC 3 cut(s) 732, 853, 1045
BsiSI CCGG 1 cut(s) 186
BslFI GGGAC 5 cut(s) 40, 155, 269, 789, 1352
BslI CCNNNNNNNGG 1 cut(s) 414
BsmAI GTCTC 3 cut(s) 151, 1080, 1327
BsmBI CGTCTC 1 cut(s) 151
BsmFI GGGAC 5 cut(s) 40, 155, 269, 789, 1352
BsmI GAATGC 2 cut(s) 638, 1049
BsnI GGCC 3 cut(s) 156, 438, 1000
Bsp1286I GDGCHC 3 cut(s) 732, 853, 1045
Bsp143I GATC 2 cut(s) 583, 696
Bsp19I CCATGG 1 cut(s) 1182
BspANI GGCC 3 cut(s) 156, 438, 1000
BspCNI CTCAG 2 cut(s) 764, 898
BspFNI CGCG 1 cut(s) 810
BspLI GGNNCC 4 cut(s) 202, 633, 805, 999
BspMAI CTGCAG 1 cut(s) 1417
BspPI GGATC 2 cut(s) 591, 691
BsrDI GCAATG 2 cut(s) 433, 1104
BsrI ACTGG 3 cut(s) 289, 371, 922
BssECI CCNNGG 5 cut(s) 157, 184, 799, 808, 1182
BssMI GATC 2 cut(s) 583, 696
BssNI GRCGYC 1 cut(s) 240
BssT1I CCWWGG 1 cut(s) 1182
Bst2UI CCWGG 2 cut(s) 409, 801
Bst4CI ACNGT 5 cut(s) 536, 690, 1075, 1091, 1352
BstACI GRCGYC 1 cut(s) 240
BstAPI GCANNNNNTGC 4 cut(s) 261, 952, 967, 1169
BstC8I GCNNGC 1 cut(s) 636
BstDEI CTNAG 4 cut(s) 751, 906, 1023, 1401
BstDSI CCRYGG 2 cut(s) 808, 1182
BstF5I GGATG 5 cut(s) 516, 876, 1085, 1119, 1267
BstFNI CGCG 1 cut(s) 810
BstH2I RGCGCY 1 cut(s) 192
BstHHI GCGC 1 cut(s) 191
BstKTI GATC 2 cut(s) 586, 699
BstMAI GTCTC 3 cut(s) 151, 1080, 1327
BstMBI GATC 2 cut(s) 583, 696
BstMCI CGRYCG 2 cut(s) 95, 313
BstNI CCWGG 2 cut(s) 409, 801
BstPAI GACNNNNGTC 1 cut(s) 801
BstSCI CCNGG 3 cut(s) 184, 407, 799
BstSFI CTRYAG 3 cut(s) 396, 756, 1413
BstSLI GKGCMC 1 cut(s) 1045
BstUI CGCG 1 cut(s) 810
BstV1I GCAGC 6 cut(s) 391, 410, 413, 958, 1087, 1159
BstV2I GAAGAC 1 cut(s) 58
BstX2I RGATCY 1 cut(s) 696
BstYI RGATCY 1 cut(s) 696
BsuRI GGCC 3 cut(s) 156, 438, 1000
BtgI CCRYGG 2 cut(s) 808, 1182
BtsCI GGATG 5 cut(s) 516, 876, 1085, 1119, 1267
BtsI GCAGTG 1 cut(s) 1170
BtsIMutI CAGTG 3 cut(s) 522, 1071, 1170
Cac8I GCNNGC 1 cut(s) 636
CfoI GCGC 1 cut(s) 191
Cfr13I GGNCC 3 cut(s) 331, 803, 998
Cfr42I CCGCGG 1 cut(s) 811
CseI GACGC 1 cut(s) 253
Csp6I GTAC 4 cut(s) 41, 665, 831, 1226
CspCI CAANNNNNGTGG 2 cut(s) 1146, 1181
CviAII CATG 7 cut(s) 38, 328, 440, 599, 1066, 1183, 1437
CviQI GTAC 4 cut(s) 41, 665, 831, 1226
DdeI CTNAG 4 cut(s) 751, 906, 1023, 1401
DpnI GATC 2 cut(s) 585, 698
DpnII GATC 2 cut(s) 583, 696
DrdI GACNNNNNNGTC 1 cut(s) 239
DseDI GACNNNNNNGTC 1 cut(s) 239
EaeI YGGCCR 1 cut(s) 436
EciI GGCGGA 1 cut(s) 331
Ecl136II GAGCTC 1 cut(s) 730
Eco130I CCWWGG 1 cut(s) 1182
Eco147I AGGCCT 1 cut(s) 156
Eco24I GRGCYC 1 cut(s) 732
Eco32I GATATC 1 cut(s) 271
Eco47I GGWCC 2 cut(s) 331, 803
Eco53kI GAGCTC 1 cut(s) 730
Eco57I CTGAAG 2 cut(s) 858, 879
EcoICRI GAGCTC 1 cut(s) 730
EcoRII CCWGG 2 cut(s) 407, 799
EcoRV GATATC 1 cut(s) 271
EcoT14I CCWWGG 1 cut(s) 1182
EcoT38I GRGCYC 1 cut(s) 732
ErhI CCWWGG 1 cut(s) 1182
Esp3I CGTCTC 1 cut(s) 151
FaeI CATG 7 cut(s) 41, 331, 443, 602, 1069, 1186, 1440
FalI AAGNNNNNCTT 4 cut(s) 532, 564, 837, 869
FaqI GGGAC 5 cut(s) 40, 155, 269, 789, 1352
FatI CATG 7 cut(s) 37, 327, 439, 598, 1065, 1182, 1436
FauI CCCGC 2 cut(s) 117, 815
FauNDI CATATG 1 cut(s) 1111
FokI GGATG 5 cut(s) 523, 883, 1092, 1106, 1274
FriOI GRGCYC 1 cut(s) 732
FspBI CTAG 2 cut(s) 81, 1421
GlaI GCGC 1 cut(s) 190
GsaI CCCAGC 1 cut(s) 574
HaeII RGCGCY 1 cut(s) 192
HaeIII GGCC 3 cut(s) 156, 438, 1000
HapII CCGG 1 cut(s) 186
HgaI GACGC 1 cut(s) 253
HhaI GCGC 1 cut(s) 191
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 7 cut(s) 41, 331, 443, 602, 1069, 1186, 1440
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HincII GTYRAC 1 cut(s) 651
HindII GTYRAC 1 cut(s) 651
HindIII AAGCTT 2 cut(s) 321, 1242
HinfI GANTC 2 cut(s) 495, 908
HpaII CCGG 1 cut(s) 186
HphI GGTGA 2 cut(s) 110, 994
Hpy166II GTNNAC 3 cut(s) 651, 833, 1043
Hpy188I TCNGA 3 cut(s) 214, 898, 1390
Hpy188III TCNNGA 2 cut(s) 485, 1307
Hpy8I GTNNAC 3 cut(s) 651, 833, 1043
Hpy99I CGWCG 4 cut(s) 242, 245, 269, 1216
HpyAV CCTTC 2 cut(s) 485, 1372
HpyCH4III ACNGT 5 cut(s) 536, 690, 1075, 1091, 1352
HpyCH4IV ACGT 4 cut(s) 240, 282, 879, 1214
HpyF3I CTNAG 4 cut(s) 751, 906, 1023, 1401
HpySE526I ACGT 4 cut(s) 240, 282, 879, 1214
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 7 cut(s) 41, 331, 443, 602, 1069, 1186, 1440
HspAI GCGC 1 cut(s) 189
KspI CCGCGG 1 cut(s) 811
Kzo9I GATC 2 cut(s) 583, 696
LmnI GCTCC 3 cut(s) 206, 631, 848
Lsp1109I GCAGC 6 cut(s) 391, 410, 413, 958, 1087, 1159
LweI GCATC 5 cut(s) 171, 295, 517, 1041, 1070
MaeI CTAG 2 cut(s) 81, 1421
MaeII ACGT 4 cut(s) 240, 282, 879, 1214
MaeIII GTNAC 2 cut(s) 875, 1346
MalI GATC 2 cut(s) 585, 698
MboI GATC 2 cut(s) 583, 696
MboII GAAGA 6 cut(s) 58, 473, 540, 851, 875, 878
MfeI CAATTG 2 cut(s) 432, 1152
MflI RGATCY 1 cut(s) 696
MhlI GDGCHC 3 cut(s) 732, 853, 1045
MlsI TGGCCA 1 cut(s) 438
MluNI TGGCCA 1 cut(s) 438
MlyI GAGTC 1 cut(s) 917
MmeI TCCRAC 3 cut(s) 757, 1057, 1197
Mox20I TGGCCA 1 cut(s) 438
MroXI GAANNNNTTC 1 cut(s) 1395
MscI TGGCCA 1 cut(s) 438
MseI TTAA 5 cut(s) 228, 855, 924, 990, 1254
MslI CAYNNNNRTG 1 cut(s) 565
Msp20I TGGCCA 1 cut(s) 438
MspA1I CMGCKG 5 cut(s) 404, 755, 810, 1319, 1357
MspI CCGG 1 cut(s) 186
MspR9I CCNGG 3 cut(s) 186, 409, 801
MunI CAATTG 2 cut(s) 432, 1152
Mva1269I GAATGC 2 cut(s) 638, 1049
MvaI CCWGG 2 cut(s) 409, 801
MvnI CGCG 1 cut(s) 810
NciI CCSGG 1 cut(s) 186
NcoI CCATGG 1 cut(s) 1182
NdeI CATATG 1 cut(s) 1111
NdeII GATC 2 cut(s) 583, 696
NlaIII CATG 7 cut(s) 41, 331, 443, 602, 1069, 1186, 1440
NlaIV GGNNCC 4 cut(s) 202, 633, 805, 999
NmeAIII GCCGAG 2 cut(s) 138, 231
NmuCI GTSAC 2 cut(s) 875, 1346
OliI CACNNNNGTG 1 cut(s) 565
PceI AGGCCT 1 cut(s) 156
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 279
PctI GAATGC 2 cut(s) 638, 1049
PdmI GAANNNNTTC 1 cut(s) 1395
PfeI GAWTC 1 cut(s) 495
PleI GAGTC 1 cut(s) 916
PpsI GAGTC 1 cut(s) 916
PshAI GACNNNNGTC 1 cut(s) 801
Psp124BI GAGCTC 1 cut(s) 732
Psp6I CCWGG 2 cut(s) 407, 799
PspFI CCCAGC 1 cut(s) 570
PspGI CCWGG 2 cut(s) 407, 799
PspN4I GGNNCC 4 cut(s) 202, 633, 805, 999
PspPI GGNCC 3 cut(s) 331, 803, 998
PsrI GAACNNNNNNTAC 2 cut(s) 449, 481
PstI CTGCAG 1 cut(s) 1417
PsuI RGATCY 1 cut(s) 696
PvuII CAGCTG 4 cut(s) 404, 755, 1319, 1357
RsaI GTAC 4 cut(s) 42, 666, 832, 1227
RsaNI GTAC 4 cut(s) 41, 665, 831, 1226
RseI CAYNNNNRTG 1 cut(s) 565
SacI GAGCTC 1 cut(s) 732
SacII CCGCGG 1 cut(s) 811
SaqAI TTAA 5 cut(s) 228, 855, 924, 990, 1254
Sau3AI GATC 2 cut(s) 583, 696
Sau96I GGNCC 3 cut(s) 331, 803, 998
ScaI AGTACT 1 cut(s) 1227
SchI GAGTC 1 cut(s) 917
ScrFI CCNGG 3 cut(s) 186, 409, 801
SduI GDGCHC 3 cut(s) 732, 853, 1045
SfaNI GCATC 5 cut(s) 171, 295, 517, 1041, 1070
SfcI CTRYAG 3 cut(s) 396, 756, 1413
Sfr303I CCGCGG 1 cut(s) 811
SgrBI CCGCGG 1 cut(s) 811
SinI GGWCC 2 cut(s) 331, 803
SmiMI CAYNNNNRTG 1 cut(s) 565
SmlI CTYRAG 1 cut(s) 956
SmoI CTYRAG 1 cut(s) 956
SseBI AGGCCT 1 cut(s) 156
SspI AATATT 1 cut(s) 1284
SspMI CTAG 2 cut(s) 81, 1421
SstI GAGCTC 1 cut(s) 732
StuI AGGCCT 1 cut(s) 156
StyD4I CCNGG 3 cut(s) 184, 407, 799
StyI CCWWGG 1 cut(s) 1182
TaaI ACNGT 5 cut(s) 536, 690, 1075, 1091, 1352
TaiI ACGT 4 cut(s) 243, 285, 882, 1217
TaqI TCGA 4 cut(s) 237, 267, 613, 795
TatI WGTACW 1 cut(s) 1225
TauI GCSGC 4 cut(s) 298, 301, 318, 352
TfiI GAWTC 1 cut(s) 495
Tru1I TTAA 5 cut(s) 228, 855, 924, 990, 1254
Tru9I TTAA 5 cut(s) 228, 855, 924, 990, 1254
TscAI CASTG 3 cut(s) 529, 1078, 1170
TseFI GTSAC 2 cut(s) 875, 1346
TseI GCWGC 6 cut(s) 401, 404, 423, 946, 1100, 1172
Tsp45I GTSAC 2 cut(s) 875, 1346
TspDTI ATGAA 7 cut(s) 465, 504, 527, 570, 1108, 1293, 1305
TspGWI ACGGA 4 cut(s) 52, 133, 157, 652
TspRI CASTG 3 cut(s) 529, 1078, 1170
VneI GTGCAC 1 cut(s) 1041
VpaK11BI GGWCC 2 cut(s) 331, 803
XapI RAATTY 2 cut(s) 1268, 1294
XmnI GAANNNNTTC 1 cut(s) 1395
XspI CTAG 2 cut(s) 81, 1421
ZraI GACGTC 1 cut(s) 241
ZrmI AGTACT 1 cut(s) 1227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.