Rh6DG311800

pfkB family carbohydrate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0009506
Physical Location & Seq
Forward (+)
51324349 .. 51328112
3764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG311800.1

Sequence Viewer

Length: 1110 bp
ATGCACACCATAACCCTCAAAACCCCAATCCCCTACCATGTACCCTCCCTTAAGTCCCACTCCATCTTCCCCCAAAACCCTAGACTAACCCGACCCCACCTCATTTTCCCGCCAATTCACCACCGCCCCCACTCTCACTCCTCCGTCACCAAAGGCCTCGGCATCTCCGTCCCCAACTCCACCCCCGGCGCTCTCAACGGCTCCCTCCACTCCGAAAACCTCGCCGTTAAGGACATCGACGTCGCAACTCTCGGCAATCTCTGCGTCGATATCGTCCTCAACGTCCCCCAGTTGCCGCCGCCCGATGCCGACCGCCGCAAAGCTTACATGGACCGCCTATCCGCCTCGCCGCCCGATAAGAAATACTGGGAAGCTGGTGGCAACTGCAATATGGCTATAGCAGCTGCCAGGTTGGGGCTTCGCTGCAATGCAATTGGCCATGTGGGTAATGAAGTGTATGGCCGGTTCTTATTAGATGTTCTTCGTGATGAAGGAATCGGTATGGTTGGGATGAATGAGGATACTGATGCTAACAGTTCAAGTGCTTCTTCGTATGAAACACTTTTGTGCTGGGTTCTTGTGGATCAGTTGCAAAGACATGGTTTTTGTAGTCGAGCTGATTTCAGTAAGGAGCCTGCATTCAGTTGGTTGACCAAATTATCCGTACAAGTAAAGACGGCGATAAAACAGTCAAAGATCCTGTTCTGTAATGGTTATGGCTTTGACGAGCTCTCTCCTGCTGTAATAGCCTCAGCTGTAGAGTATGCTGTTGAAGTTGGAACAGCAATTTTTTTCGACCCTGGACCCCGCGGAAAGAGCCTCTCTGCTGGTACACCTGAAGAACAAGGAGCACTTAACCTGTTGTTGAGGATGAGTGACGTTCTTCTTCTAACTTCAGATGAGGCTGAGTCATTGACTGGCATTAAAAACCCGATATTAGCAGGGCAGCAGTTGCTCAAGCAAGGGGTGCAAACAAAATGGGTGATTGTTAAAATGGGGCCAAAGGGTTCAATTCTCATAACTAAGTCTGGCATCTCTTGTGCACCAGCATTCAAGGTATCACTTTCTAACTTGACATTCTCTTATGACGTTGCCTTACTTTTTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

369

Amino Acids

39.93

Weight (kDa)

8.11

Isoelectric Point (pI)

34.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 122 - 353 2.2e-19 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 239
AatII GACGTC 1 cut(s) 243
AccII CGCG 1 cut(s) 810
AclWI GGATC 2 cut(s) 591, 691
AcoI YGGCCR 2 cut(s) 436, 460
AcuI CTGAAG 2 cut(s) 858, 879
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 3 cut(s) 42, 666, 832
AfiI CCNNNNNNNGG 1 cut(s) 414
AflII CTTAAG 1 cut(s) 50
AgsI TTSAA 4 cut(s) 540, 773, 1011, 1054
AjnI CCWGG 2 cut(s) 407, 799
AleI CACNNNNGTG 1 cut(s) 565
AluBI AGCT 6 cut(s) 323, 374, 404, 617, 730, 755
AluI AGCT 6 cut(s) 323, 374, 404, 617, 730, 755
Alw21I GWGCWC 3 cut(s) 732, 853, 1045
Alw44I GTGCAC 1 cut(s) 1041
AlwI GGATC 2 cut(s) 591, 691
AoxI GGCC 4 cut(s) 154, 436, 460, 998
ApaLI GTGCAC 1 cut(s) 1041
ApeKI GCWGC 4 cut(s) 401, 404, 423, 946
ArsI GACNNNNNNTTYG 2 cut(s) 588, 620
AspLEI GCGC 1 cut(s) 191
AspS9I GGNCC 3 cut(s) 331, 803, 998
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 3 cut(s) 110, 139, 994
AvaII GGWCC 2 cut(s) 331, 803
BaeGI GKGCMC 1 cut(s) 1045
BalI TGGCCA 1 cut(s) 438
BanII GRGCYC 1 cut(s) 732
Bbv12I GWGCWC 3 cut(s) 732, 853, 1045
BbvCI CCTCAGC 1 cut(s) 751
BbvI GCAGC 4 cut(s) 391, 410, 413, 958
BccI CCATC 1 cut(s) 71
BceAI ACGGC 3 cut(s) 209, 214, 693
BciT130I CCWGG 2 cut(s) 409, 801
BciVI GTATCC 1 cut(s) 514
BcnI CCSGG 1 cut(s) 186
BfaI CTAG 2 cut(s) 81, 1108
BfmI CTRYAG 2 cut(s) 396, 756
BfoI RGCGCY 1 cut(s) 192
BfrI CTTAAG 1 cut(s) 50
BfuI GTATCC 1 cut(s) 514
BisI GCNGC 8 cut(s) 296, 299, 316, 350, 402, 405, 424, 947
BlsI GCNGC 8 cut(s) 297, 300, 317, 351, 403, 406, 425, 948
Bme1390I CCNGG 3 cut(s) 186, 409, 801
Bme18I GGWCC 2 cut(s) 331, 803
BmgT120I GGNCC 3 cut(s) 331, 803, 998
BmiI GGNNCC 4 cut(s) 202, 633, 805, 999
BmrFI CCNGG 3 cut(s) 186, 409, 801
BmrI ACTGGG 2 cut(s) 283, 376
BmsI GCATC 4 cut(s) 171, 295, 517, 1041
BmuI ACTGGG 2 cut(s) 283, 376
Bpu10I CCTNAGC 1 cut(s) 751
BpuEI CTTGAG 1 cut(s) 941
BpuMI CCSGG 1 cut(s) 186
BsaHI GRCGYC 1 cut(s) 240
BsaJI CCNNGG 4 cut(s) 157, 184, 799, 808
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 1 cut(s) 414
Bse118I RCCGGY 1 cut(s) 462
Bse1I ACTGG 3 cut(s) 289, 371, 922
Bse3DI GCAATG 1 cut(s) 433
BseBI CCWGG 2 cut(s) 409, 801
BseDI CCNNGG 4 cut(s) 157, 184, 799, 808
BseGI GGATG 2 cut(s) 516, 876
BseLI CCNNNNNNNGG 1 cut(s) 414
BseMI GCAATG 1 cut(s) 433
BseMII CTCAG 2 cut(s) 765, 897
BseNI ACTGG 3 cut(s) 289, 371, 922
BseRI GAGGAG 1 cut(s) 130
BseSI GKGCMC 1 cut(s) 1045
BseXI GCAGC 4 cut(s) 391, 410, 413, 958
BseYI CCCAGC 1 cut(s) 570
Bsh1236I CGCG 1 cut(s) 810
Bsh1285I CGRYCG 1 cut(s) 313
BshFI GGCC 4 cut(s) 156, 438, 462, 1000
BsiEI CGRYCG 1 cut(s) 313
BsiHKAI GWGCWC 3 cut(s) 732, 853, 1045
BsiSI CCGG 2 cut(s) 186, 463
BslFI GGGAC 3 cut(s) 40, 155, 269
BslI CCNNNNNNNGG 1 cut(s) 414
BsmFI GGGAC 3 cut(s) 40, 155, 269
BsmI GAATGC 2 cut(s) 638, 1049
BsnI GGCC 4 cut(s) 156, 438, 462, 1000
Bsp1286I GDGCHC 3 cut(s) 732, 853, 1045
Bsp143I GATC 2 cut(s) 583, 696
BspANI GGCC 4 cut(s) 156, 438, 462, 1000
BspCNI CTCAG 2 cut(s) 764, 898
BspFNI CGCG 1 cut(s) 810
BspLI GGNNCC 4 cut(s) 202, 633, 805, 999
BspPI GGATC 2 cut(s) 591, 691
BspTI CTTAAG 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 433
BsrFI RCCGGY 1 cut(s) 462
BsrI ACTGG 3 cut(s) 289, 371, 922
BssAI RCCGGY 1 cut(s) 462
BssECI CCNNGG 4 cut(s) 157, 184, 799, 808
BssMI GATC 2 cut(s) 583, 696
BssNI GRCGYC 1 cut(s) 240
Bst2UI CCWGG 2 cut(s) 409, 801
Bst4CI ACNGT 2 cut(s) 536, 690
BstACI GRCGYC 1 cut(s) 240
BstAFI CTTAAG 1 cut(s) 50
BstAPI GCANNNNNTGC 3 cut(s) 261, 952, 967
BstC8I GCNNGC 1 cut(s) 636
BstDEI CTNAG 3 cut(s) 751, 906, 1023
BstDSI CCRYGG 1 cut(s) 808
BstF5I GGATG 2 cut(s) 516, 876
BstFNI CGCG 1 cut(s) 810
BstH2I RGCGCY 1 cut(s) 192
BstHHI GCGC 1 cut(s) 191
BstKTI GATC 2 cut(s) 586, 699
BstMBI GATC 2 cut(s) 583, 696
BstMCI CGRYCG 1 cut(s) 313
BstMWI GCNNNNNNNGC 6 cut(s) 261, 401, 746, 816, 952, 967
BstNI CCWGG 2 cut(s) 409, 801
BstSCI CCNGG 3 cut(s) 184, 407, 799
BstSFI CTRYAG 2 cut(s) 396, 756
BstSLI GKGCMC 1 cut(s) 1045
BstUI CGCG 1 cut(s) 810
BstV1I GCAGC 4 cut(s) 391, 410, 413, 958
BstX2I RGATCY 1 cut(s) 696
BstYI RGATCY 1 cut(s) 696
BsuI GTATCC 1 cut(s) 514
BsuRI GGCC 4 cut(s) 156, 438, 462, 1000
BtgI CCRYGG 1 cut(s) 808
BtsCI GGATG 2 cut(s) 516, 876
Cac8I GCNNGC 1 cut(s) 636
CfoI GCGC 1 cut(s) 191
Cfr10I RCCGGY 1 cut(s) 462
Cfr13I GGNCC 3 cut(s) 331, 803, 998
Cfr42I CCGCGG 1 cut(s) 811
CseI GACGC 1 cut(s) 253
Csp6I GTAC 3 cut(s) 41, 665, 831
CviAII CATG 4 cut(s) 38, 328, 440, 599
CviQI GTAC 3 cut(s) 41, 665, 831
DdeI CTNAG 3 cut(s) 751, 906, 1023
DpnI GATC 2 cut(s) 585, 698
DpnII GATC 2 cut(s) 583, 696
DrdI GACNNNNNNGTC 1 cut(s) 239
DseDI GACNNNNNNGTC 1 cut(s) 239
EaeI YGGCCR 2 cut(s) 436, 460
EciI GGCGGA 1 cut(s) 331
Ecl136II GAGCTC 1 cut(s) 730
Eco147I AGGCCT 1 cut(s) 156
Eco24I GRGCYC 1 cut(s) 732
Eco32I GATATC 1 cut(s) 271
Eco47I GGWCC 2 cut(s) 331, 803
Eco53kI GAGCTC 1 cut(s) 730
Eco57I CTGAAG 2 cut(s) 858, 879
EcoICRI GAGCTC 1 cut(s) 730
EcoRII CCWGG 2 cut(s) 407, 799
EcoRV GATATC 1 cut(s) 271
EcoT38I GRGCYC 1 cut(s) 732
FaeI CATG 4 cut(s) 41, 331, 443, 602
FalI AAGNNNNNCTT 4 cut(s) 532, 564, 837, 869
FaqI GGGAC 3 cut(s) 40, 155, 269
FatI CATG 4 cut(s) 37, 327, 439, 598
FauI CCCGC 2 cut(s) 117, 815
Fnu4HI GCNGC 8 cut(s) 296, 299, 316, 350, 402, 405, 424, 947
FokI GGATG 2 cut(s) 523, 883
FriOI GRGCYC 1 cut(s) 732
Fsp4HI GCNGC 8 cut(s) 296, 299, 316, 350, 402, 405, 424, 947
FspBI CTAG 2 cut(s) 81, 1108
GlaI GCGC 1 cut(s) 190
GluI GCNGC 8 cut(s) 296, 299, 316, 350, 402, 405, 424, 947
GsaI CCCAGC 1 cut(s) 574
HaeII RGCGCY 1 cut(s) 192
HaeIII GGCC 4 cut(s) 156, 438, 462, 1000
HapII CCGG 2 cut(s) 186, 463
HgaI GACGC 1 cut(s) 253
HhaI GCGC 1 cut(s) 191
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 4 cut(s) 41, 331, 443, 602
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HincII GTYRAC 1 cut(s) 651
HindII GTYRAC 1 cut(s) 651
HindIII AAGCTT 1 cut(s) 321
HinfI GANTC 2 cut(s) 495, 908
HpaII CCGG 2 cut(s) 186, 463
HphI GGTGA 3 cut(s) 110, 139, 994
Hpy166II GTNNAC 3 cut(s) 651, 833, 1043
Hpy188I TCNGA 2 cut(s) 214, 898
Hpy188III TCNNGA 1 cut(s) 485
Hpy8I GTNNAC 3 cut(s) 651, 833, 1043
Hpy99I CGWCG 3 cut(s) 242, 245, 269
HpyAV CCTTC 1 cut(s) 485
HpyCH4III ACNGT 2 cut(s) 536, 690
HpyCH4IV ACGT 4 cut(s) 240, 282, 879, 1089
HpyCH4V TGCA 8 cut(s) 4, 387, 426, 431, 592, 638, 970, 1043
HpyF10VI GCNNNNNNNGC 6 cut(s) 261, 401, 746, 816, 952, 967
HpyF3I CTNAG 3 cut(s) 751, 906, 1023
HpySE526I ACGT 4 cut(s) 240, 282, 879, 1089
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 4 cut(s) 41, 331, 443, 602
HspAI GCGC 1 cut(s) 189
KspI CCGCGG 1 cut(s) 811
Kzo9I GATC 2 cut(s) 583, 696
LmnI GCTCC 3 cut(s) 206, 631, 848
Lsp1109I GCAGC 4 cut(s) 391, 410, 413, 958
LweI GCATC 4 cut(s) 171, 295, 517, 1041
MaeI CTAG 2 cut(s) 81, 1108
MaeII ACGT 4 cut(s) 240, 282, 879, 1089
MaeIII GTNAC 2 cut(s) 145, 875
MalI GATC 2 cut(s) 585, 698
MboI GATC 2 cut(s) 583, 696
MboII GAAGA 6 cut(s) 58, 473, 540, 851, 875, 878
MfeI CAATTG 1 cut(s) 432
MflI RGATCY 1 cut(s) 696
MhlI GDGCHC 3 cut(s) 732, 853, 1045
MlsI TGGCCA 1 cut(s) 438
MluCI AATT 5 cut(s) 114, 432, 656, 786, 1011
MluNI TGGCCA 1 cut(s) 438
MlyI GAGTC 1 cut(s) 917
MmeI TCCRAC 1 cut(s) 757
Mox20I TGGCCA 1 cut(s) 438
MscI TGGCCA 1 cut(s) 438
MseI TTAA 5 cut(s) 51, 228, 855, 924, 990
MslI CAYNNNNRTG 1 cut(s) 565
Msp20I TGGCCA 1 cut(s) 438
MspA1I CMGCKG 3 cut(s) 404, 755, 810
MspCI CTTAAG 1 cut(s) 50
MspI CCGG 2 cut(s) 186, 463
MspR9I CCNGG 3 cut(s) 186, 409, 801
MunI CAATTG 1 cut(s) 432
Mva1269I GAATGC 2 cut(s) 638, 1049
MvaI CCWGG 2 cut(s) 409, 801
MvnI CGCG 1 cut(s) 810
MwoI GCNNNNNNNGC 6 cut(s) 261, 401, 746, 816, 952, 967
NciI CCSGG 1 cut(s) 186
NdeII GATC 2 cut(s) 583, 696
NlaIII CATG 4 cut(s) 41, 331, 443, 602
NlaIV GGNNCC 4 cut(s) 202, 633, 805, 999
NmeAIII GCCGAG 2 cut(s) 138, 231
NmuCI GTSAC 2 cut(s) 145, 875
OliI CACNNNNGTG 1 cut(s) 565
PceI AGGCCT 1 cut(s) 156
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 279
PctI GAATGC 2 cut(s) 638, 1049
PfeI GAWTC 1 cut(s) 495
PkrI GCNGC 8 cut(s) 297, 300, 317, 351, 403, 406, 425, 948
PleI GAGTC 1 cut(s) 916
PpsI GAGTC 1 cut(s) 916
Psp124BI GAGCTC 1 cut(s) 732
Psp6I CCWGG 2 cut(s) 407, 799
PspFI CCCAGC 1 cut(s) 570
PspGI CCWGG 2 cut(s) 407, 799
PspN4I GGNNCC 4 cut(s) 202, 633, 805, 999
PspPI GGNCC 3 cut(s) 331, 803, 998
PsrI GAACNNNNNNTAC 2 cut(s) 449, 481
PsuI RGATCY 1 cut(s) 696
PvuII CAGCTG 2 cut(s) 404, 755
RsaI GTAC 3 cut(s) 42, 666, 832
RsaNI GTAC 3 cut(s) 41, 665, 831
RseI CAYNNNNRTG 1 cut(s) 565
SacI GAGCTC 1 cut(s) 732
SacII CCGCGG 1 cut(s) 811
SaqAI TTAA 5 cut(s) 51, 228, 855, 924, 990
SatI GCNGC 8 cut(s) 296, 299, 316, 350, 402, 405, 424, 947
Sau3AI GATC 2 cut(s) 583, 696
Sau96I GGNCC 3 cut(s) 331, 803, 998
SchI GAGTC 1 cut(s) 917
ScrFI CCNGG 3 cut(s) 186, 409, 801
SduI GDGCHC 3 cut(s) 732, 853, 1045
SfaNI GCATC 4 cut(s) 171, 295, 517, 1041
SfcI CTRYAG 2 cut(s) 396, 756
Sfr303I CCGCGG 1 cut(s) 811
SgrBI CCGCGG 1 cut(s) 811
SinI GGWCC 2 cut(s) 331, 803
SmiMI CAYNNNNRTG 1 cut(s) 565
SmlI CTYRAG 2 cut(s) 50, 956
SmoI CTYRAG 2 cut(s) 50, 956
Sse9I AATT 5 cut(s) 114, 432, 656, 786, 1011
SseBI AGGCCT 1 cut(s) 156
SspMI CTAG 2 cut(s) 81, 1108
SstI GAGCTC 1 cut(s) 732
StuI AGGCCT 1 cut(s) 156
StyD4I CCNGG 3 cut(s) 184, 407, 799
TaaI ACNGT 2 cut(s) 536, 690
TaiI ACGT 4 cut(s) 243, 285, 882, 1092
TaqI TCGA 4 cut(s) 237, 267, 613, 795
TasI AATT 5 cut(s) 114, 432, 656, 786, 1011
TauI GCSGC 4 cut(s) 298, 301, 318, 352
TfiI GAWTC 1 cut(s) 495
Tru1I TTAA 5 cut(s) 51, 228, 855, 924, 990
Tru9I TTAA 5 cut(s) 51, 228, 855, 924, 990
TseFI GTSAC 2 cut(s) 145, 875
TseI GCWGC 4 cut(s) 401, 404, 423, 946
Tsp45I GTSAC 2 cut(s) 145, 875
TspDTI ATGAA 4 cut(s) 465, 504, 527, 570
TspGWI ACGGA 3 cut(s) 133, 157, 652
Vha464I CTTAAG 1 cut(s) 50
VneI GTGCAC 1 cut(s) 1041
VpaK11BI GGWCC 2 cut(s) 331, 803
XspI CTAG 2 cut(s) 81, 1108
ZraI GACGTC 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.