AT1G12450

transmembrane protein 64-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007106
Physical Location & Seq
Forward (+)
4243904 .. 4245993
2090 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G12450.1

Sequence Viewer

Length: 912 bp
ATGACGTACAGCAATGGCGTCGATGGGACGGTGCCTGAGCTGAAATTGAGAGTCGAAGAAGATTCCGATAATGGTGATTATCTGAAATTGAGAGGAGGATCAAATGAAGAAGATGAAGGATCATCGGCGGAATCATCGAGATGTCCGATAGGATCGGTTACTTCTGTCTGGTTCTGGGTGAAATTGATCTCCTTGGTCGTTTGTTTGGGTTCATTGGCCTTTGTTATCATCAAATGGGTTGGTCCCTTTTTGATTGAAAAGGAGCTGATTCCATTTATAAATTGGGTGAGAAACACATTCAGCATACCGGTTCTCGGTCTTCTACTATTTGCCTCAGTGGCATTGTTTCCAAGCATTCTTCTTCCTTCTTCCCCTTCCATGTGGATGGCTGGTCTTACATTTGGTTATGGAAAAGGGTTTCTTCTGATTTTATCAGCTGCTTCCATCGGTGTTACTCTTCCTTTCTTAATTGGACATCTCTTCCTCCACAAGATGCAAGAATGGTTGAAGAAATACCCTAAAAAAGCAGCCATACTTAGAGCTGCTGGTGAAGGAACCTGGTTTCATCAGTTTCAAGCAGTCACACTAATCCGTGTCTCTCCATTTCCTTACATCATTTACAACTACTGCGCTTTAGCGACTGGAGTTCATTATGGTCCTTATATCTTAGGCTCTCTTGTTGGAATGGTTCCTGAGATCTTTGTCTCAATTTACACGGGTATAATGCTAAGGACACTAGCAGTTGCATCAGACACGAGACACACACTTTCGGTTGTGGAGATAGTCGTGAATGTTCTTGGCTTCTGTGTAACTGCGAGCGCGACTATAGTCTGCACAATCTATGCGAAGAAGAAGCTAAGTGCGATGCAATCAGAGGACGTAGAGACATTACAAAATCCAATACTTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

303

Amino Acids

33.3

Weight (kDa)

6.95

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 121 - 241 8.9e-17 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 278
AccB1I GGYRCC 1 cut(s) 31
AccII CGCG 1 cut(s) 821
AciI CCGC 1 cut(s) 128
AclWI GGATC 3 cut(s) 106, 127, 160
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 1 cut(s) 314
AgeI ACCGGT 1 cut(s) 307
AgsI TTSAA 3 cut(s) 257, 508, 575
AjnI CCWGG 1 cut(s) 557
AluBI AGCT 5 cut(s) 40, 265, 437, 542, 856
AluI AGCT 5 cut(s) 40, 265, 437, 542, 856
Alw26I GTCTC 4 cut(s) 601, 709, 751, 878
AlwI GGATC 3 cut(s) 106, 127, 160
AoxI GGCC 1 cut(s) 216
ApeKI GCWGC 3 cut(s) 437, 527, 542
AsiGI ACCGGT 1 cut(s) 307
AspLEI GCGC 2 cut(s) 632, 821
AspS9I GGNCC 2 cut(s) 242, 656
AsuHPI GGTGA 4 cut(s) 86, 190, 298, 560
AvaII GGWCC 2 cut(s) 242, 656
BanI GGYRCC 1 cut(s) 31
BauI CACGAG 1 cut(s) 754
BbsI GAAGAC 1 cut(s) 311
BbvI GCAGC 3 cut(s) 424, 529, 539
BccI CCATC 3 cut(s) 17, 379, 452
BcgI CGANNNNNNTGC 1 cut(s) 35
BciT130I CCWGG 1 cut(s) 559
BcoDI GTCTC 4 cut(s) 601, 709, 751, 878
BfaI CTAG 1 cut(s) 737
BfmI CTRYAG 1 cut(s) 825
BglI GCCNNNNNGGC 1 cut(s) 338
BglII AGATCT 1 cut(s) 696
BisI GCNGC 3 cut(s) 438, 528, 543
BlsI GCNGC 3 cut(s) 439, 529, 544
Bme1390I CCNGG 1 cut(s) 559
Bme18I GGWCC 2 cut(s) 242, 656
BmgT120I GGNCC 2 cut(s) 242, 656
BmiI GGNNCC 4 cut(s) 33, 244, 556, 690
BmrFI CCNGG 1 cut(s) 559
BmsI GCATC 3 cut(s) 483, 755, 855
BoxI GACNNNNGTC 1 cut(s) 827
BpiI GAAGAC 1 cut(s) 311
BpmI CTGGAG 1 cut(s) 663
Bpu10I CCTNAGC 2 cut(s) 36, 728
BsaHI GRCGYC 1 cut(s) 18
BsaJI CCNNGG 1 cut(s) 192
BsaWI WCCGGW 1 cut(s) 307
Bsc4I CCNNNNNNNGG 1 cut(s) 314
Bse118I RCCGGY 1 cut(s) 307
Bse1I ACTGG 1 cut(s) 646
Bse3DI GCAATG 1 cut(s) 19
BseBI CCWGG 1 cut(s) 559
BseDI CCNNGG 1 cut(s) 192
BseGI GGATG 1 cut(s) 390
BseLI CCNNNNNNNGG 1 cut(s) 314
BseMI GCAATG 1 cut(s) 19
BseMII CTCAG 3 cut(s) 27, 348, 684
BseNI ACTGG 1 cut(s) 646
BseRI GAGGAG 1 cut(s) 108
BseXI GCAGC 3 cut(s) 424, 529, 539
BsgI GTGCAG 1 cut(s) 817
Bsh1236I CGCG 1 cut(s) 821
BshFI GGCC 1 cut(s) 218
BshNI GGYRCC 1 cut(s) 31
BshTI ACCGGT 1 cut(s) 307
BsiSI CCGG 1 cut(s) 308
BslFI GGGAC 2 cut(s) 40, 228
BslI CCNNNNNNNGG 1 cut(s) 314
BsmAI GTCTC 4 cut(s) 601, 709, 751, 878
BsmFI GGGAC 2 cut(s) 40, 228
BsmI GAATGC 1 cut(s) 354
BsnI GGCC 1 cut(s) 218
Bsp143I GATC 5 cut(s) 98, 119, 152, 186, 696
BspACI CCGC 1 cut(s) 128
BspANI GGCC 1 cut(s) 218
BspCNI CTCAG 3 cut(s) 28, 347, 685
BspFNI CGCG 1 cut(s) 821
BspLI GGNNCC 4 cut(s) 33, 244, 556, 690
BspPI GGATC 3 cut(s) 106, 127, 160
BspT107I GGYRCC 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 19
BsrFI RCCGGY 1 cut(s) 307
BsrI ACTGG 1 cut(s) 646
BssAI RCCGGY 1 cut(s) 307
BssECI CCNNGG 1 cut(s) 192
BssMI GATC 5 cut(s) 98, 119, 152, 186, 696
BssNI GRCGYC 1 cut(s) 18
BssSI CACGAG 1 cut(s) 754
BssT1I CCWWGG 1 cut(s) 192
Bst2BI CACGAG 1 cut(s) 754
Bst2UI CCWGG 1 cut(s) 559
Bst4CI ACNGT 1 cut(s) 31
Bst6I CTCTTC 2 cut(s) 462, 485
BstACI GRCGYC 1 cut(s) 18
BstC8I GCNNGC 1 cut(s) 817
BstDEI CTNAG 7 cut(s) 36, 334, 536, 667, 693, 728, 857
BstF5I GGATG 1 cut(s) 390
BstFNI CGCG 1 cut(s) 821
BstHHI GCGC 2 cut(s) 632, 821
BstKTI GATC 5 cut(s) 101, 122, 155, 189, 699
BstMAI GTCTC 4 cut(s) 601, 709, 751, 878
BstMBI GATC 5 cut(s) 98, 119, 152, 186, 696
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstNI CCWGG 1 cut(s) 559
BstPAI GACNNNNGTC 1 cut(s) 827
BstSCI CCNGG 1 cut(s) 557
BstSFI CTRYAG 1 cut(s) 825
BstUI CGCG 1 cut(s) 821
BstV1I GCAGC 3 cut(s) 424, 529, 539
BstV2I GAAGAC 1 cut(s) 311
BstX2I RGATCY 1 cut(s) 696
BstXI CCANNNNNNTGG 1 cut(s) 385
BstYI RGATCY 1 cut(s) 696
BsuRI GGCC 1 cut(s) 218
BtgZI GCGATG 1 cut(s) 878
BtsCI GGATG 1 cut(s) 390
BtsIMutI CAGTG 1 cut(s) 342
Cac8I GCNNGC 1 cut(s) 817
CfoI GCGC 2 cut(s) 632, 821
Cfr10I RCCGGY 1 cut(s) 307
Cfr13I GGNCC 2 cut(s) 242, 656
CseI GACGC 1 cut(s) 7
CsiI ACCWGGT 1 cut(s) 557
Csp6I GTAC 1 cut(s) 7
CspAI ACCGGT 1 cut(s) 307
CviAII CATG 1 cut(s) 379
CviQI GTAC 1 cut(s) 7
DdeI CTNAG 7 cut(s) 36, 334, 536, 667, 693, 728, 857
DpnI GATC 5 cut(s) 100, 121, 154, 188, 698
DpnII GATC 5 cut(s) 98, 119, 152, 186, 696
Eam1104I CTCTTC 2 cut(s) 462, 485
EarI CTCTTC 2 cut(s) 462, 485
EciI GGCGGA 1 cut(s) 143
Eco130I CCWWGG 1 cut(s) 192
Eco47I GGWCC 2 cut(s) 242, 656
EcoRII CCWGG 1 cut(s) 557
EcoT14I CCWWGG 1 cut(s) 192
ErhI CCWWGG 1 cut(s) 192
FaeI CATG 1 cut(s) 382
FalI AAGNNNNNCTT 2 cut(s) 405, 437
FaqI GGGAC 2 cut(s) 40, 228
FatI CATG 1 cut(s) 378
Fnu4HI GCNGC 3 cut(s) 438, 528, 543
FokI GGATG 1 cut(s) 397
Fsp4HI GCNGC 3 cut(s) 438, 528, 543
FspBI CTAG 1 cut(s) 737
GlaI GCGC 2 cut(s) 631, 820
GluI GCNGC 3 cut(s) 438, 528, 543
GsuI CTGGAG 1 cut(s) 663
HaeIII GGCC 1 cut(s) 218
HapII CCGG 1 cut(s) 308
HgaI GACGC 1 cut(s) 7
HhaI GCGC 2 cut(s) 632, 821
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 1 cut(s) 382
Hin6I GCGC 2 cut(s) 630, 819
HinP1I GCGC 2 cut(s) 630, 819
HinfI GANTC 4 cut(s) 51, 62, 131, 268
HpaII CCGG 1 cut(s) 308
HphI GGTGA 4 cut(s) 86, 190, 298, 560
Hpy188I TCNGA 6 cut(s) 67, 84, 147, 426, 751, 874
Hpy188III TCNNGA 3 cut(s) 138, 692, 787
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 4 cut(s) 110, 375, 384, 545
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4IV ACGT 2 cut(s) 5, 879
HpyCH4V TGCA 4 cut(s) 496, 746, 834, 868
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpyF3I CTNAG 7 cut(s) 36, 334, 536, 667, 693, 728, 857
HpySE526I ACGT 2 cut(s) 5, 879
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 1 cut(s) 382
HspAI GCGC 2 cut(s) 630, 819
Kzo9I GATC 5 cut(s) 98, 119, 152, 186, 696
LmnI GCTCC 1 cut(s) 262
Lsp1109I GCAGC 3 cut(s) 424, 529, 539
LweI GCATC 3 cut(s) 483, 755, 855
MabI ACCWGGT 1 cut(s) 557
MaeI CTAG 1 cut(s) 737
MaeII ACGT 2 cut(s) 5, 879
MaeIII GTNAC 4 cut(s) 157, 451, 580, 808
MalI GATC 5 cut(s) 100, 121, 154, 188, 698
MboI GATC 5 cut(s) 98, 119, 152, 186, 696
MflI RGATCY 1 cut(s) 696
MluCI AATT 6 cut(s) 44, 86, 182, 280, 468, 708
MlyI GAGTC 1 cut(s) 60
MmeI TCCRAC 1 cut(s) 661
MnlI CCTC 5 cut(s) 86, 89, 343, 494, 868
MseI TTAA 2 cut(s) 467, 910
MslI CAYNNNNRTG 2 cut(s) 139, 383
MspA1I CMGCKG 1 cut(s) 437
MspI CCGG 1 cut(s) 308
MspR9I CCNGG 1 cut(s) 559
Mva1269I GAATGC 1 cut(s) 354
MvaI CCWGG 1 cut(s) 559
MvnI CGCG 1 cut(s) 821
MwoI GCNNNNNNNGC 1 cut(s) 338
NdeII GATC 5 cut(s) 98, 119, 152, 186, 696
NlaIII CATG 1 cut(s) 382
NlaIV GGNNCC 4 cut(s) 33, 244, 556, 690
NmuCI GTSAC 1 cut(s) 580
PcsI WCGNNNNNNNCGW 1 cut(s) 143
PctI GAATGC 1 cut(s) 354
PfeI GAWTC 3 cut(s) 62, 131, 268
PinAI ACCGGT 1 cut(s) 307
PkrI GCNGC 3 cut(s) 439, 529, 544
PleI GAGTC 1 cut(s) 59
PpsI GAGTC 1 cut(s) 59
PshAI GACNNNNGTC 1 cut(s) 827
PsiI TTATAA 1 cut(s) 278
Psp6I CCWGG 1 cut(s) 557
PspGI CCWGG 1 cut(s) 557
PspN4I GGNNCC 4 cut(s) 33, 244, 556, 690
PspPI GGNCC 2 cut(s) 242, 656
PsuI RGATCY 1 cut(s) 696
PvuII CAGCTG 1 cut(s) 437
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
RseI CAYNNNNRTG 2 cut(s) 139, 383
SaqAI TTAA 2 cut(s) 467, 910
SatI GCNGC 3 cut(s) 438, 528, 543
Sau3AI GATC 5 cut(s) 98, 119, 152, 186, 696
Sau96I GGNCC 2 cut(s) 242, 656
SchI GAGTC 1 cut(s) 60
ScrFI CCNGG 1 cut(s) 559
SetI ASST 8 cut(s) 8, 42, 267, 439, 544, 560, 858, 882
SexAI ACCWGGT 1 cut(s) 557
SfaNI GCATC 3 cut(s) 483, 755, 855
SfcI CTRYAG 1 cut(s) 825
SinI GGWCC 2 cut(s) 242, 656
SmiMI CAYNNNNRTG 2 cut(s) 139, 383
Sse9I AATT 6 cut(s) 44, 86, 182, 280, 468, 708
SsiI CCGC 1 cut(s) 128
SspMI CTAG 1 cut(s) 737
StyD4I CCNGG 1 cut(s) 557
StyI CCWWGG 1 cut(s) 192
TaaI ACNGT 1 cut(s) 31
TaiI ACGT 2 cut(s) 8, 882
TaqI TCGA 3 cut(s) 21, 54, 137
TaqII GACCGA 1 cut(s) 305
TasI AATT 6 cut(s) 44, 86, 182, 280, 468, 708
TfiI GAWTC 3 cut(s) 62, 131, 268
Tru1I TTAA 2 cut(s) 467, 910
Tru9I TTAA 2 cut(s) 467, 910
TscAI CASTG 1 cut(s) 342
TseFI GTSAC 1 cut(s) 580
TseI GCWGC 3 cut(s) 437, 527, 542
Tsp45I GTSAC 1 cut(s) 580
TspDTI ATGAA 5 cut(s) 120, 129, 201, 554, 638
TspGWI ACGGA 1 cut(s) 581
TspRI CASTG 1 cut(s) 342
VpaK11BI GGWCC 2 cut(s) 242, 656
XcmI CCANNNNNNNNNTGG 1 cut(s) 279
XspI CTAG 1 cut(s) 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.