AT4G12000

transmembrane protein 64-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007106
Physical Location & Seq
Reverse (-)
7192533 .. 7195216
2684 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G12000.1

Sequence Viewer

Length: 921 bp
ATGACTTATCACGAAGAAGACGATACAGTTTCTGAATTTCGAGTTAGAGTTGAAGAAGATGGAGTTGATAAATTAGGTCATTATGTTAAACTTACTGAAGATTTCGAAGTACATCGACAAGAAACAGAACAAGAATCTTCATCATCACCGTCTTCTTCTTCTTCATGTGGACAGAAACGATCTGTTTGGTTTTGGATCAAGCTTGGTCTTTTCTTCACTTTTCTAACTGCTTTGGGTCTCGCTGGTTACAAATGGCTATACCCTTTAATCATGGATAAGGAGCTAATCCCACTCATAAAATGGGAGATGGAGACATTTACACACCCTGTGTGCGGTATTCTTGTGTTTGCATCAGTCTCTTTATTCCCTGTGATACTTATTCCAACTACGCCGTCAATGTGGGTGGCTGGGATTACATTTGGTTATTTCTATGGCCTTCTCCTTACTCTTCCAGCTGTAGCTATTGGTGTTTCACTTCCTTACTTCATCAGCTATCTCTTCCTCAACAAAATTCAAGGTTGGTTAGAAAGATATCCAGATCAAGCTGCAATGTTGAGAGCTGCTGGTGGAGGCAGTTGGTTTCATCAGTTTCGTGCAGTTACCTTAATCCGGATTTCTCCATTTCCTTTTGCTGTATATAATTACTGCGCTGTTGCAACTCGTGTGAAGTTCGGTCCTTACATGGCTGGTTCTCTCGTAGGCATGGCGCCGGAGATTTTTGTCGCAATTTATACAGGGATTCTTATAAGGACATTGGCAGATGCATCCACTGCGGAACAAAAGGGTCTCTCGATTCTTCAAATTGTTCTCAACATTTTTGGTTTTGTAGCAACTGTTGTGACAACTGTTCTCATCACTAAGTATGCGAAAAGACAGCTTGAAGTTATGAAGAAGGAGAAGGAAGCTTTGTTGTTACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

306

Amino Acids

34.44

Weight (kDa)

6.53

Isoelectric Point (pI)

49.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 127 - 247 4.3e-15 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 746
AccB1I GGYRCC 1 cut(s) 706
AccIII TCCGGA 1 cut(s) 609
AciI CCGC 2 cut(s) 333, 773
AclWI GGATC 1 cut(s) 203
AcsI RAATTY 2 cut(s) 35, 510
AcuI CTGAAG 1 cut(s) 117
AcyI GRCGYC 1 cut(s) 707
AdeI CACNNNGTG 1 cut(s) 328
AfaI GTAC 1 cut(s) 111
AfiI CCNNNNNNNGG 2 cut(s) 332, 609
AgsI TTSAA 4 cut(s) 53, 515, 800, 881
AluBI AGCT 9 cut(s) 202, 283, 455, 461, 492, 545, 560, 877, 905
AluI AGCT 9 cut(s) 202, 283, 455, 461, 492, 545, 560, 877, 905
Alw26I GTCTC 4 cut(s) 242, 305, 361, 791
AlwI GGATC 1 cut(s) 203
AlwNI CAGNNNCTG 1 cut(s) 32
Aor13HI TCCGGA 1 cut(s) 609
AoxI GGCC 1 cut(s) 433
ApeKI GCWGC 2 cut(s) 545, 560
ApoI RAATTY 2 cut(s) 35, 510
AspLEI GCGC 2 cut(s) 650, 709
AspS9I GGNCC 1 cut(s) 674
AsuHPI GGTGA 1 cut(s) 138
AsuII TTCGAA 1 cut(s) 105
AvaII GGWCC 1 cut(s) 674
BanI GGYRCC 1 cut(s) 706
BauI CACGAG 1 cut(s) 660
BbsI GAAGAC 2 cut(s) 24, 144
BbvI GCAGC 2 cut(s) 532, 547
BccI CCATC 2 cut(s) 53, 301
BceAI ACGGC 1 cut(s) 376
BcoDI GTCTC 4 cut(s) 242, 305, 361, 791
BfmI CTRYAG 1 cut(s) 456
BfoI RGCGCY 1 cut(s) 710
BisI GCNGC 2 cut(s) 546, 561
BlsI GCNGC 2 cut(s) 547, 562
Bme18I GGWCC 1 cut(s) 674
BmgT120I GGNCC 1 cut(s) 674
BmiI GGNNCC 1 cut(s) 708
BmsI GCATC 3 cut(s) 359, 751, 773
BpiI GAAGAC 2 cut(s) 24, 144
Bpu14I TTCGAA 1 cut(s) 105
BsaHI GRCGYC 1 cut(s) 707
BsaI GGTCTC 2 cut(s) 242, 791
BsaWI WCCGGW 1 cut(s) 609
Bsc4I CCNNNNNNNGG 2 cut(s) 332, 609
Bse3DI GCAATG 1 cut(s) 555
BseAI TCCGGA 1 cut(s) 609
BseGI GGATG 1 cut(s) 764
BseLI CCNNNNNNNGG 2 cut(s) 332, 609
BseMI GCAATG 1 cut(s) 555
BseXI GCAGC 2 cut(s) 532, 547
BseYI CCCAGC 1 cut(s) 407
BsgI GTGCAG 1 cut(s) 615
BshFI GGCC 1 cut(s) 435
BshNI GGYRCC 1 cut(s) 706
BsiSI CCGG 2 cut(s) 610, 710
BslI CCNNNNNNNGG 2 cut(s) 332, 609
BsmAI GTCTC 4 cut(s) 242, 305, 361, 791
BsnI GGCC 1 cut(s) 435
Bso31I GGTCTC 2 cut(s) 242, 791
Bsp119I TTCGAA 1 cut(s) 105
Bsp13I TCCGGA 1 cut(s) 609
Bsp143I GATC 3 cut(s) 179, 195, 538
BspACI CCGC 2 cut(s) 333, 773
BspANI GGCC 1 cut(s) 435
BspEI TCCGGA 1 cut(s) 609
BspLI GGNNCC 1 cut(s) 708
BspPI GGATC 1 cut(s) 203
BspT104I TTCGAA 1 cut(s) 105
BspT107I GGYRCC 1 cut(s) 706
BspTNI GGTCTC 2 cut(s) 242, 791
BsrDI GCAATG 1 cut(s) 555
BssMI GATC 3 cut(s) 179, 195, 538
BssNI GRCGYC 1 cut(s) 707
BssSI CACGAG 1 cut(s) 660
Bst2BI CACGAG 1 cut(s) 660
Bst4CI ACNGT 5 cut(s) 28, 150, 835, 847, 918
Bst6I CTCTTC 2 cut(s) 453, 503
BstACI GRCGYC 1 cut(s) 707
BstAPI GCANNNNNTGC 1 cut(s) 770
BstBI TTCGAA 1 cut(s) 105
BstDEI CTNAG 1 cut(s) 858
BstF5I GGATG 1 cut(s) 764
BstH2I RGCGCY 1 cut(s) 710
BstHHI GCGC 2 cut(s) 650, 709
BstKTI GATC 3 cut(s) 182, 198, 541
BstMAI GTCTC 4 cut(s) 242, 305, 361, 791
BstMBI GATC 3 cut(s) 179, 195, 538
BstMWI GCNNNNNNNGC 1 cut(s) 770
BstSFI CTRYAG 1 cut(s) 456
BstV1I GCAGC 2 cut(s) 532, 547
BstV2I GAAGAC 2 cut(s) 24, 144
BsuRI GGCC 1 cut(s) 435
BtsCI GGATG 1 cut(s) 764
BtsI GCAGTG 1 cut(s) 768
BtsIMutI CAGTG 1 cut(s) 768
CaiI CAGNNNCTG 1 cut(s) 32
CfoI GCGC 2 cut(s) 650, 709
Cfr13I GGNCC 1 cut(s) 674
Csp6I GTAC 1 cut(s) 110
CspCI CAANNNNNGTGG 2 cut(s) 384, 419
CviAII CATG 4 cut(s) 165, 271, 682, 703
CviQI GTAC 1 cut(s) 110
DdeI CTNAG 1 cut(s) 858
DinI GGCGCC 1 cut(s) 708
DpnI GATC 3 cut(s) 181, 197, 540
DpnII GATC 3 cut(s) 179, 195, 538
DraIII CACNNNGTG 1 cut(s) 328
Eam1104I CTCTTC 2 cut(s) 453, 503
EarI CTCTTC 2 cut(s) 453, 503
Eco31I GGTCTC 2 cut(s) 242, 791
Eco32I GATATC 1 cut(s) 533
Eco47I GGWCC 1 cut(s) 674
Eco57I CTGAAG 1 cut(s) 117
EcoRV GATATC 1 cut(s) 533
EcoT22I ATGCAT 1 cut(s) 766
EgeI GGCGCC 1 cut(s) 708
EheI GGCGCC 1 cut(s) 708
FaeI CATG 4 cut(s) 168, 274, 685, 706
FatI CATG 4 cut(s) 164, 270, 681, 702
Fnu4HI GCNGC 2 cut(s) 546, 561
FokI GGATG 1 cut(s) 751
Fsp4HI GCNGC 2 cut(s) 546, 561
GlaI GCGC 2 cut(s) 649, 708
GluI GCNGC 2 cut(s) 546, 561
GsaI CCCAGC 1 cut(s) 411
HaeII RGCGCY 1 cut(s) 710
HaeIII GGCC 1 cut(s) 435
HapII CCGG 2 cut(s) 610, 710
HhaI GCGC 2 cut(s) 650, 709
Hin1I GRCGYC 1 cut(s) 707
Hin1II CATG 4 cut(s) 168, 274, 685, 706
Hin6I GCGC 2 cut(s) 648, 707
HinP1I GCGC 2 cut(s) 648, 707
HindIII AAGCTT 2 cut(s) 200, 903
HinfI GANTC 3 cut(s) 134, 739, 793
HpaII CCGG 2 cut(s) 610, 710
HphI GGTGA 1 cut(s) 138
Hpy166II GTNNAC 1 cut(s) 170
Hpy188I TCNGA 1 cut(s) 34
Hpy188III TCNNGA 4 cut(s) 11, 536, 610, 790
Hpy8I GTNNAC 1 cut(s) 170
HpyAV CCTTC 3 cut(s) 446, 886, 892
HpyCH4III ACNGT 5 cut(s) 28, 150, 835, 847, 918
HpyCH4V TGCA 5 cut(s) 350, 548, 596, 656, 764
HpyF10VI GCNNNNNNNGC 1 cut(s) 770
HpyF3I CTNAG 1 cut(s) 858
Hsp92I GRCGYC 1 cut(s) 707
Hsp92II CATG 4 cut(s) 168, 274, 685, 706
HspAI GCGC 2 cut(s) 648, 707
KasI GGCGCC 1 cut(s) 706
Kpn2I TCCGGA 1 cut(s) 609
Kzo9I GATC 3 cut(s) 179, 195, 538
LmnI GCTCC 1 cut(s) 280
Lsp1109I GCAGC 2 cut(s) 532, 547
LweI GCATC 3 cut(s) 359, 751, 773
MaeIII GTNAC 4 cut(s) 245, 598, 838, 912
MalI GATC 3 cut(s) 181, 197, 540
MboI GATC 3 cut(s) 179, 195, 538
MluCI AATT 6 cut(s) 35, 71, 510, 640, 726, 801
Mly113I GGCGCC 1 cut(s) 707
MmeI TCCRAC 1 cut(s) 407
MnlI CCTC 2 cut(s) 512, 563
Mph1103I ATGCAT 1 cut(s) 766
MroI TCCGGA 1 cut(s) 609
MseI TTAA 3 cut(s) 87, 266, 605
MspA1I CMGCKG 1 cut(s) 455
MspI CCGG 2 cut(s) 610, 710
MwoI GCNNNNNNNGC 1 cut(s) 770
NarI GGCGCC 1 cut(s) 707
NdeII GATC 3 cut(s) 179, 195, 538
NlaIII CATG 4 cut(s) 168, 274, 685, 706
NlaIV GGNNCC 1 cut(s) 708
NmuCI GTSAC 1 cut(s) 838
NsiI ATGCAT 1 cut(s) 766
NspV TTCGAA 1 cut(s) 105
PcsI WCGNNNNNNNCGW 1 cut(s) 18
PfeI GAWTC 3 cut(s) 134, 739, 793
PkrI GCNGC 2 cut(s) 547, 562
PluTI GGCGCC 1 cut(s) 710
PsiI TTATAA 1 cut(s) 746
PspFI CCCAGC 1 cut(s) 407
PspN4I GGNNCC 1 cut(s) 708
PspPI GGNCC 1 cut(s) 674
PstNI CAGNNNCTG 1 cut(s) 32
PvuII CAGCTG 1 cut(s) 455
RsaI GTAC 1 cut(s) 111
RsaNI GTAC 1 cut(s) 110
SaqAI TTAA 3 cut(s) 87, 266, 605
SatI GCNGC 2 cut(s) 546, 561
Sau3AI GATC 3 cut(s) 179, 195, 538
Sau96I GGNCC 1 cut(s) 674
SfaNI GCATC 3 cut(s) 359, 751, 773
SfcI CTRYAG 1 cut(s) 456
SfoI GGCGCC 1 cut(s) 708
SfuI TTCGAA 1 cut(s) 105
SinI GGWCC 1 cut(s) 674
Sse9I AATT 6 cut(s) 35, 71, 510, 640, 726, 801
SsiI CCGC 2 cut(s) 333, 773
SspDI GGCGCC 1 cut(s) 706
TaaI ACNGT 5 cut(s) 28, 150, 835, 847, 918
TaqI TCGA 4 cut(s) 40, 105, 115, 791
TaqII GACCGA 1 cut(s) 662
TasI AATT 6 cut(s) 35, 71, 510, 640, 726, 801
TatI WGTACW 1 cut(s) 109
TfiI GAWTC 3 cut(s) 134, 739, 793
Tru1I TTAA 3 cut(s) 87, 266, 605
Tru9I TTAA 3 cut(s) 87, 266, 605
TscAI CASTG 1 cut(s) 775
TseFI GTSAC 1 cut(s) 838
TseI GCWGC 2 cut(s) 545, 560
Tsp45I GTSAC 1 cut(s) 838
TspDTI ATGAA 5 cut(s) 129, 153, 475, 572, 902
TspRI CASTG 1 cut(s) 775
VpaK11BI GGWCC 1 cut(s) 674
XapI RAATTY 2 cut(s) 35, 510
XcmI CCANNNNNNNNNTGG 1 cut(s) 297
Zsp2I ATGCAT 1 cut(s) 766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.