Rroxscaffold_3G00256010

transmembrane protein 64-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0007106
Physical Location & Seq
Forward (+)
50454565 .. 50458062
3498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00256010.1

Sequence Viewer

Length: 1002 bp
ATGACCTATGAGGAGAATGTTGATCGAGGAGAGGTGGAGCCGGAGCTGATCGTTAGAATTGGGGCCGGTGAGGATGATGAGAAGGGGAGAGGGGACTATGTGAAGCTCAGGGAGTGTCCCGGCGAGGCTGAGGAGTCGTCGTCGTCGTCTTCGCCCAAGGGATTATTGTGTTTGTGGTATTGGGTTAGGTTGGTGTTGCTGTTCATGTGCTTGGGGTTGTTGGCTGCGGTTTTTATCAAATGGGTTGGGCCTTTTCTGATGGATACGGAGGTTATTCCAATGATTAACTGGGAGACGAAAACATTCAGTCCTCCAGTGCTTGCTATTCTGGTCTTTGCTTCTGTGGCATTGTTTCCTACCATACTATTACCATCTTCACCTTCTATGTGGGTGGCTGGAATGACTTTTGGTTATGGCTATGGATTTCTTCTCATTATATCAGCAGCATCTATTGGTATATCACTTCCATATTTCATTAGCTATCTCTTCTACCATAAAATTCAGGGATGGTTAGAAAAATATCCAAAGAGAGCTTCCATTCTAAGATCAGCTGGTGAAGGAAACTGGTTTCACCAGTTTCGAGCTGTAGCATTAATCAGGATTTCTCCTTTCCCTTATATTATATATAACTACTGTGCTGTGGCAACCAATGTCAAGTATGGTCCGTACCTCTTGGGGTCCTTGGCGGGAATGGTTCCAGAAACATTTGTTGCCATTTACACTGTCTCCAGTGTCTCCAGTATCTATACTGGCAAGTCATCATCTCCAGTGTCTCCAGTATCTATACTGGCAAGTCATCATCTCCAGTGTCTTATCACATTTGGGATCTTAATACGGACATTGGCAGATGCTTCACATGAGCACCACGGCCTTTCAGCCCCACAGATAATCTTCACTGTTGTTGGCTTTGCTTTGGCTGTGGCTACCACAATTTTCTTCACAGTGTATGCAAAAAAGCAGCTCAAGGAATTGCAGAAGGAAGATGAGCCACTGTTGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

333

Amino Acids

36.91

Weight (kDa)

6.18

Isoelectric Point (pI)

45.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 123 - 243 3.4e-15 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 227, 686
AclWI GGATC 1 cut(s) 833
AcsI RAATTY 1 cut(s) 498
AfaI GTAC 1 cut(s) 668
AjuI GAANNNNNNNTTGG 2 cut(s) 517, 549
AluBI AGCT 7 cut(s) 46, 106, 480, 533, 551, 584, 961
AluI AGCT 7 cut(s) 46, 106, 480, 533, 551, 584, 961
Alw21I GWGCWC 1 cut(s) 864
Alw26I GTCTC 4 cut(s) 287, 730, 739, 777
AlwI GGATC 1 cut(s) 833
AoxI GGCC 3 cut(s) 63, 248, 868
ApeKI GCWGC 3 cut(s) 224, 443, 958
ApoI RAATTY 1 cut(s) 498
AseI ATTAAT 1 cut(s) 593
Asp700I GAANNNNTTC 1 cut(s) 302
AspS9I GGNCC 4 cut(s) 63, 248, 662, 678
AsuC2I CCSGG 1 cut(s) 120
AsuHPI GGTGA 4 cut(s) 80, 369, 563, 566
AvaII GGWCC 2 cut(s) 662, 678
BaeI ACNNNNGTAYC 2 cut(s) 255, 288
BbsI GAAGAC 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 864
BbvCI CCTCAGC 1 cut(s) 129
BbvI GCAGC 3 cut(s) 211, 455, 970
BccI CCATC 3 cut(s) 253, 379, 501
BceAI ACGGC 1 cut(s) 883
BciVI GTATCC 1 cut(s) 256
BcnI CCSGG 1 cut(s) 120
BcoDI GTCTC 4 cut(s) 287, 730, 739, 777
BfmI CTRYAG 1 cut(s) 585
BfuI GTATCC 1 cut(s) 256
BisI GCNGC 3 cut(s) 225, 444, 959
BlsI GCNGC 3 cut(s) 226, 445, 960
Bme1390I CCNGG 1 cut(s) 120
Bme18I GGWCC 2 cut(s) 662, 678
BmgT120I GGNCC 4 cut(s) 63, 248, 662, 678
BmiI GGNNCC 4 cut(s) 39, 64, 679, 696
BmrFI CCNGG 1 cut(s) 120
BmrI ACTGGG 1 cut(s) 298
BmsI GCATC 2 cut(s) 455, 838
BmuI ACTGGG 1 cut(s) 298
BpiI GAAGAC 1 cut(s) 141
BpmI CTGGAG 6 cut(s) 297, 712, 721, 750, 759, 788
Bpu10I CCTNAGC 2 cut(s) 107, 129
BpuEI CTTGAG 1 cut(s) 947
BpuMI CCSGG 1 cut(s) 120
BsaJI CCNNGG 3 cut(s) 156, 681, 865
BsaXI ACNNNNNCTCC 4 cut(s) 125, 155, 710, 740
Bse118I RCCGGY 1 cut(s) 65
BseDI CCNNGG 3 cut(s) 156, 681, 865
BseGI GGATG 2 cut(s) 79, 512
BseMII CTCAG 2 cut(s) 120, 121
BseRI GAGGAG 3 cut(s) 26, 42, 146
BseXI GCAGC 3 cut(s) 211, 455, 970
BshFI GGCC 3 cut(s) 65, 250, 870
BsiHKAI GWGCWC 1 cut(s) 864
BsiSI CCGG 3 cut(s) 41, 66, 120
BslFI GGGAC 2 cut(s) 102, 107
BsmAI GTCTC 4 cut(s) 287, 730, 739, 777
BsmBI CGTCTC 1 cut(s) 287
BsmFI GGGAC 2 cut(s) 102, 107
BsnI GGCC 3 cut(s) 65, 250, 870
Bsp1286I GDGCHC 1 cut(s) 864
Bsp143I GATC 4 cut(s) 22, 48, 545, 825
BspACI CCGC 2 cut(s) 227, 686
BspANI GGCC 3 cut(s) 65, 250, 870
BspCNI CTCAG 2 cut(s) 120, 121
BspLI GGNNCC 4 cut(s) 39, 64, 679, 696
BspPI GGATC 1 cut(s) 833
BsrFI RCCGGY 1 cut(s) 65
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 3 cut(s) 156, 681, 865
BssMI GATC 4 cut(s) 22, 48, 545, 825
BssT1I CCWWGG 2 cut(s) 156, 681
Bst4CI ACNGT 5 cut(s) 635, 724, 898, 943, 993
Bst6I CTCTTC 1 cut(s) 491
BstC8I GCNNGC 1 cut(s) 321
BstDEI CTNAG 3 cut(s) 107, 129, 542
BstDSI CCRYGG 1 cut(s) 865
BstF5I GGATG 2 cut(s) 79, 512
BstKTI GATC 4 cut(s) 25, 51, 548, 828
BstMAI GTCTC 4 cut(s) 287, 730, 739, 777
BstMBI GATC 4 cut(s) 22, 48, 545, 825
BstMWI GCNNNNNNNGC 2 cut(s) 344, 994
BstSCI CCNGG 1 cut(s) 118
BstSFI CTRYAG 1 cut(s) 585
BstV1I GCAGC 3 cut(s) 211, 455, 970
BstV2I GAAGAC 1 cut(s) 141
BstX2I RGATCY 1 cut(s) 825
BstYI RGATCY 1 cut(s) 825
BsuI GTATCC 1 cut(s) 256
BsuRI GGCC 3 cut(s) 65, 250, 870
BtgI CCRYGG 1 cut(s) 865
BtsCI GGATG 2 cut(s) 79, 512
BtsIMutI CAGTG 8 cut(s) 321, 720, 736, 774, 812, 894, 948, 989
Cac8I GCNNGC 1 cut(s) 321
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 4 cut(s) 63, 248, 662, 678
Csp6I GTAC 1 cut(s) 667
CviAII CATG 2 cut(s) 205, 857
CviQI GTAC 1 cut(s) 667
DdeI CTNAG 3 cut(s) 107, 129, 542
DpnI GATC 4 cut(s) 24, 50, 547, 827
DpnII GATC 4 cut(s) 22, 48, 545, 825
Eam1104I CTCTTC 1 cut(s) 491
EarI CTCTTC 1 cut(s) 491
Eco130I CCWWGG 2 cut(s) 156, 681
Eco47I GGWCC 2 cut(s) 662, 678
EcoO109I RGGNCCY 1 cut(s) 678
EcoT14I CCWWGG 2 cut(s) 156, 681
ErhI CCWWGG 2 cut(s) 156, 681
Esp3I CGTCTC 1 cut(s) 287
FaeI CATG 2 cut(s) 208, 860
FaqI GGGAC 2 cut(s) 102, 107
FatI CATG 2 cut(s) 204, 856
FauI CCCGC 1 cut(s) 679
Fnu4HI GCNGC 3 cut(s) 225, 444, 959
FokI GGATG 2 cut(s) 86, 519
Fsp4HI GCNGC 3 cut(s) 225, 444, 959
GluI GCNGC 3 cut(s) 225, 444, 959
GsuI CTGGAG 6 cut(s) 297, 712, 721, 750, 759, 788
HaeIII GGCC 3 cut(s) 65, 250, 870
HapII CCGG 3 cut(s) 41, 66, 120
Hin1II CATG 2 cut(s) 208, 860
HinfI GANTC 1 cut(s) 134
HpaII CCGG 3 cut(s) 41, 66, 120
HphI GGTGA 4 cut(s) 80, 369, 563, 566
Hpy188I TCNGA 1 cut(s) 258
Hpy188III TCNNGA 2 cut(s) 598, 698
Hpy99I CGWCG 3 cut(s) 142, 145, 148
HpyAV CCTTC 4 cut(s) 76, 390, 551, 970
HpyCH4III ACNGT 5 cut(s) 635, 724, 898, 943, 993
HpyCH4V TGCA 3 cut(s) 950, 973, 997
HpyF10VI GCNNNNNNNGC 2 cut(s) 344, 994
HpyF3I CTNAG 3 cut(s) 107, 129, 542
Hsp92II CATG 2 cut(s) 208, 860
Kzo9I GATC 4 cut(s) 22, 48, 545, 825
LmnI GCTCC 2 cut(s) 37, 43
Lsp1109I GCAGC 3 cut(s) 211, 455, 970
LweI GCATC 2 cut(s) 455, 838
MalI GATC 4 cut(s) 24, 50, 547, 827
MboI GATC 4 cut(s) 22, 48, 545, 825
MboII GAAGA 7 cut(s) 141, 366, 419, 478, 883, 928, 992
MflI RGATCY 1 cut(s) 825
MhlI GDGCHC 1 cut(s) 864
MluCI AATT 4 cut(s) 57, 498, 930, 968
MlyI GAGTC 1 cut(s) 143
MroXI GAANNNNTTC 1 cut(s) 302
MseI TTAA 3 cut(s) 285, 593, 830
MspA1I CMGCKG 1 cut(s) 551
MspI CCGG 3 cut(s) 41, 66, 120
MspR9I CCNGG 1 cut(s) 120
MwoI GCNNNNNNNGC 2 cut(s) 344, 994
NciI CCSGG 1 cut(s) 120
NdeII GATC 4 cut(s) 22, 48, 545, 825
NlaIII CATG 2 cut(s) 208, 860
NlaIV GGNNCC 4 cut(s) 39, 64, 679, 696
PcsI WCGNNNNNNNCGW 1 cut(s) 143
PdmI GAANNNNTTC 1 cut(s) 302
PkrI GCNGC 3 cut(s) 226, 445, 960
PleI GAGTC 1 cut(s) 142
PpsI GAGTC 1 cut(s) 142
PpuMI RGGWCCY 1 cut(s) 678
PshBI ATTAAT 1 cut(s) 593
Psp5II RGGWCCY 1 cut(s) 678
PspN4I GGNNCC 4 cut(s) 39, 64, 679, 696
PspPI GGNCC 4 cut(s) 63, 248, 662, 678
PspPPI RGGWCCY 1 cut(s) 678
PsuI RGATCY 1 cut(s) 825
PvuII CAGCTG 1 cut(s) 551
RsaI GTAC 1 cut(s) 668
RsaNI GTAC 1 cut(s) 667
SaqAI TTAA 3 cut(s) 285, 593, 830
SatI GCNGC 3 cut(s) 225, 444, 959
Sau3AI GATC 4 cut(s) 22, 48, 545, 825
Sau96I GGNCC 4 cut(s) 63, 248, 662, 678
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 1 cut(s) 120
SduI GDGCHC 1 cut(s) 864
SfaNI GCATC 2 cut(s) 455, 838
SfcI CTRYAG 1 cut(s) 585
SinI GGWCC 2 cut(s) 662, 678
SmlI CTYRAG 1 cut(s) 962
SmoI CTYRAG 1 cut(s) 962
Sse9I AATT 4 cut(s) 57, 498, 930, 968
SsiI CCGC 2 cut(s) 227, 686
StyD4I CCNGG 1 cut(s) 118
StyI CCWWGG 2 cut(s) 156, 681
TaaI ACNGT 5 cut(s) 635, 724, 898, 943, 993
TaqI TCGA 2 cut(s) 25, 580
TasI AATT 4 cut(s) 57, 498, 930, 968
Tru1I TTAA 3 cut(s) 285, 593, 830
Tru9I TTAA 3 cut(s) 285, 593, 830
TscAI CASTG 8 cut(s) 321, 727, 736, 774, 812, 901, 948, 996
TseI GCWGC 3 cut(s) 224, 443, 958
TspDTI ATGAA 2 cut(s) 193, 463
TspGWI ACGGA 3 cut(s) 281, 654, 850
TspRI CASTG 8 cut(s) 321, 727, 736, 774, 812, 901, 948, 996
VpaK11BI GGWCC 2 cut(s) 662, 678
VspI ATTAAT 1 cut(s) 593
XapI RAATTY 1 cut(s) 498
XcmI CCANNNNNNNNNTGG 1 cut(s) 285
XmnI GAANNNNTTC 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.