pycom04g06750

transmembrane protein 64-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0007106
Physical Location & Seq
Reverse (-)
6967605 .. 6970339
2735 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g06750.1

Sequence Viewer

Length: 912 bp
ATGACCTATGACGATTGTGAGGATCGGGGAGAGGTGGTGCCGGAGCTGATTTTGAGAATTGGGGGCGAAGACGGCAAAGGGGACTATATGAAGTTGGGGGATGGTGATCGTGGCGGTTGTGGGACGGCGGAGGGGTTTGAGGCGGAGGTGGAGCCACCTTCTCCGAGAAAGTTAGCTTCTTTGTGGTATTGGGTTAGGTTGGTACTGTTGGTTATGTGCTTGGGGTTGTTGGCTGGGGTTTTTGCAAAATGGGTCGGACCATATTTCATGGATAACGAGATTATTCCAATTCTTAACTGGGAGACAAGAACATTCAGTCCTCCAGTGCTTGCAGTTCTAGTCTTTGCTTCTGTGGCATTATTTCCCACCGTACTTTTACCATCGTCACCTTCTATGTGGGTGGCTGGCATGACTTTTGGTTATGGCTATGGATTTCTTCTAATCATATCAGCAGCAGCTGTGGGTGTATCACTTCCATTTTTCGTTGGCTCTCTCTTCTACCACAAAATTCAAGGGTGGTTAGAAAAATATCCGAAGAGAGCATCCGTTTTAAGATCAGCTGGGGAAGGAGACTGGTTTCATCAGTTTCGAGCTGTAACATTAATCAGGATTTCTCCATTCCCTTACATTATCTATAATTATGCTGCTGTGGCAACAAATGTCAAGTATGTTCCTTACCTGTTAGGGTCTTTGGCGGGAATGGTGCCAGAAACATTTGCCGCCATCTACACTGGGATCCTGATACGGACGTTGGCAGACGCTTCACATGAGCGCCATGGTCTTTCAGCCCCACAGATAATCTTCACTGTAGTTGGCTTCTCTTTGACTCTGGCTACCACAATTTTCTTCACGGTGTATGCAAAAAGGCAGCTTAAGGAATTACAAAAGGACGAAGAGCCACTGCTGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

304

Amino Acids

33.55

Weight (kDa)

5.77

Isoelectric Point (pI)

41.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 126 - 246 3.1e-16 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 37, 703
AciI CCGC 5 cut(s) 114, 128, 143, 695, 720
AclWI GGATC 3 cut(s) 30, 730, 743
AcsI RAATTY 1 cut(s) 507
AfaI GTAC 2 cut(s) 204, 372
AflII CTTAAG 1 cut(s) 872
AgsI TTSAA 1 cut(s) 512
AloI GAACNNNNNNTCC 2 cut(s) 301, 333
AluBI AGCT 6 cut(s) 46, 176, 458, 560, 593, 871
AluI AGCT 6 cut(s) 46, 176, 458, 560, 593, 871
Alw26I GTCTC 2 cut(s) 296, 564
AlwI GGATC 3 cut(s) 30, 730, 743
AlwNI CAGNNNCTG 1 cut(s) 458
ApeKI GCWGC 5 cut(s) 452, 455, 644, 868, 904
ApoI RAATTY 1 cut(s) 507
AseI ATTAAT 1 cut(s) 602
AspLEI GCGC 1 cut(s) 774
AspS9I GGNCC 1 cut(s) 257
AsuHPI GGTGA 2 cut(s) 116, 378
AvaII GGWCC 1 cut(s) 257
BaeI ACNNNNGTAYC 2 cut(s) 734, 767
BamHI GGATCC 1 cut(s) 735
BanI GGYRCC 2 cut(s) 37, 703
BbsI GAAGAC 1 cut(s) 75
BbvI GCAGC 5 cut(s) 464, 467, 631, 880, 891
BccI CCATC 3 cut(s) 95, 388, 731
BceAI ACGGC 2 cut(s) 88, 141
BcoDI GTCTC 2 cut(s) 296, 564
BfaI CTAG 1 cut(s) 338
BfmI CTRYAG 1 cut(s) 807
BfoI RGCGCY 1 cut(s) 775
BfrI CTTAAG 1 cut(s) 872
BisI GCNGC 6 cut(s) 453, 456, 645, 720, 869, 905
BlsI GCNGC 6 cut(s) 454, 457, 646, 721, 870, 906
Bme18I GGWCC 1 cut(s) 257
BmgT120I GGNCC 1 cut(s) 257
BmiI GGNNCC 4 cut(s) 39, 153, 705, 737
BmrI ACTGGG 2 cut(s) 307, 741
BmsI GCATC 1 cut(s) 551
BmuI ACTGGG 2 cut(s) 307, 741
BpiI GAAGAC 1 cut(s) 75
BpmI CTGGAG 1 cut(s) 306
BsaBI GATNNNNATC 1 cut(s) 105
BsaJI CCNNGG 1 cut(s) 775
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bse1I ACTGG 4 cut(s) 302, 323, 578, 736
Bse8I GATNNNNATC 1 cut(s) 105
BseDI CCNNGG 1 cut(s) 775
BseGI GGATG 2 cut(s) 106, 542
BseJI GATNNNNATC 1 cut(s) 105
BseNI ACTGG 4 cut(s) 302, 323, 578, 736
BseXI GCAGC 5 cut(s) 464, 467, 631, 880, 891
BseYI CCCAGC 2 cut(s) 233, 560
BsgI GTGCAG 1 cut(s) 890
BshNI GGYRCC 2 cut(s) 37, 703
BsiSI CCGG 1 cut(s) 41
BslFI GGGAC 2 cut(s) 95, 136
BsmAI GTCTC 2 cut(s) 296, 564
BsmFI GGGAC 2 cut(s) 95, 136
Bsp143I GATC 4 cut(s) 22, 106, 554, 735
Bsp19I CCATGG 1 cut(s) 775
BspACI CCGC 5 cut(s) 114, 128, 143, 695, 720
BspLI GGNNCC 4 cut(s) 39, 153, 705, 737
BspPI GGATC 3 cut(s) 30, 730, 743
BspQI GCTCTTC 1 cut(s) 888
BspT107I GGYRCC 2 cut(s) 37, 703
BspTI CTTAAG 1 cut(s) 872
BsrI ACTGG 4 cut(s) 302, 323, 578, 736
BssECI CCNNGG 1 cut(s) 775
BssMI GATC 4 cut(s) 22, 106, 554, 735
BssT1I CCWWGG 1 cut(s) 775
Bst4CI ACNGT 4 cut(s) 207, 370, 808, 853
Bst6I CTCTTC 3 cut(s) 500, 530, 888
BstAFI CTTAAG 1 cut(s) 872
BstC8I GCNNGC 2 cut(s) 330, 406
BstDSI CCRYGG 1 cut(s) 775
BstF5I GGATG 2 cut(s) 106, 542
BstH2I RGCGCY 1 cut(s) 775
BstHHI GCGC 1 cut(s) 774
BstKTI GATC 4 cut(s) 25, 109, 557, 738
BstMAI GTCTC 2 cut(s) 296, 564
BstMBI GATC 4 cut(s) 22, 106, 554, 735
BstMWI GCNNNNNNNGC 4 cut(s) 72, 353, 650, 904
BstSFI CTRYAG 1 cut(s) 807
BstV1I GCAGC 5 cut(s) 464, 467, 631, 880, 891
BstV2I GAAGAC 1 cut(s) 75
BstX2I RGATCY 1 cut(s) 735
BstYI RGATCY 1 cut(s) 735
BtgI CCRYGG 1 cut(s) 775
BtsCI GGATG 2 cut(s) 106, 542
BtsI GCAGTG 1 cut(s) 899
BtsIMutI CAGTG 4 cut(s) 330, 729, 804, 899
Cac8I GCNNGC 2 cut(s) 330, 406
CaiI CAGNNNCTG 1 cut(s) 458
CfoI GCGC 1 cut(s) 774
Cfr13I GGNCC 1 cut(s) 257
CseI GACGC 1 cut(s) 767
Csp6I GTAC 2 cut(s) 203, 371
CviAII CATG 4 cut(s) 268, 409, 767, 776
CviQI GTAC 2 cut(s) 203, 371
DpnI GATC 4 cut(s) 24, 108, 556, 737
DpnII GATC 4 cut(s) 22, 106, 554, 735
Eam1104I CTCTTC 3 cut(s) 500, 530, 888
EarI CTCTTC 3 cut(s) 500, 530, 888
EciI GGCGGA 2 cut(s) 143, 158
Eco130I CCWWGG 1 cut(s) 775
Eco47I GGWCC 1 cut(s) 257
EcoT14I CCWWGG 1 cut(s) 775
ErhI CCWWGG 1 cut(s) 775
FaeI CATG 4 cut(s) 271, 412, 770, 779
FaqI GGGAC 2 cut(s) 95, 136
FatI CATG 4 cut(s) 267, 408, 766, 775
FauI CCCGC 1 cut(s) 688
Fnu4HI GCNGC 6 cut(s) 453, 456, 645, 720, 869, 905
FokI GGATG 2 cut(s) 113, 529
Fsp4HI GCNGC 6 cut(s) 453, 456, 645, 720, 869, 905
FspBI CTAG 1 cut(s) 338
GlaI GCGC 1 cut(s) 773
GluI GCNGC 6 cut(s) 453, 456, 645, 720, 869, 905
GsaI CCCAGC 2 cut(s) 237, 564
GsuI CTGGAG 1 cut(s) 306
HaeII RGCGCY 1 cut(s) 775
HapII CCGG 1 cut(s) 41
HgaI GACGC 1 cut(s) 767
HhaI GCGC 1 cut(s) 774
Hin1II CATG 4 cut(s) 271, 412, 770, 779
Hin6I GCGC 1 cut(s) 772
HinP1I GCGC 1 cut(s) 772
HinfI GANTC 1 cut(s) 826
HpaII CCGG 1 cut(s) 41
HphI GGTGA 2 cut(s) 116, 378
Hpy188I TCNGA 3 cut(s) 165, 257, 534
Hpy188III TCNNGA 2 cut(s) 607, 739
HpyAV CCTTC 3 cut(s) 168, 399, 560
HpyCH4III ACNGT 4 cut(s) 207, 370, 808, 853
HpyCH4IV ACGT 1 cut(s) 749
HpyCH4V TGCA 4 cut(s) 245, 332, 860, 907
HpyF10VI GCNNNNNNNGC 4 cut(s) 72, 353, 650, 904
HpySE526I ACGT 1 cut(s) 749
Hsp92II CATG 4 cut(s) 271, 412, 770, 779
HspAI GCGC 1 cut(s) 772
Kzo9I GATC 4 cut(s) 22, 106, 554, 735
LguI GCTCTTC 1 cut(s) 888
LmnI GCTCC 2 cut(s) 43, 151
Lsp1109I GCAGC 5 cut(s) 464, 467, 631, 880, 891
LweI GCATC 1 cut(s) 551
MaeI CTAG 1 cut(s) 338
MaeII ACGT 1 cut(s) 749
MaeIII GTNAC 2 cut(s) 384, 595
MalI GATC 4 cut(s) 24, 108, 556, 737
MboI GATC 4 cut(s) 22, 106, 554, 735
MboII GAAGA 7 cut(s) 80, 428, 487, 547, 793, 838, 905
MflI RGATCY 1 cut(s) 735
MluCI AATT 6 cut(s) 57, 288, 507, 637, 840, 878
MlyI GAGTC 1 cut(s) 820
MmeI TCCRAC 1 cut(s) 235
MnlI CCTC 6 cut(s) 13, 25, 124, 133, 139, 330
MseI TTAA 4 cut(s) 294, 551, 602, 873
MspA1I CMGCKG 2 cut(s) 458, 560
MspCI CTTAAG 1 cut(s) 872
MspI CCGG 1 cut(s) 41
MwoI GCNNNNNNNGC 4 cut(s) 72, 353, 650, 904
NcoI CCATGG 1 cut(s) 775
NdeII GATC 4 cut(s) 22, 106, 554, 735
NlaIII CATG 4 cut(s) 271, 412, 770, 779
NlaIV GGNNCC 4 cut(s) 39, 153, 705, 737
NmuCI GTSAC 1 cut(s) 384
PciSI GCTCTTC 1 cut(s) 888
PkrI GCNGC 6 cut(s) 454, 457, 646, 721, 870, 906
PleI GAGTC 1 cut(s) 820
PpsI GAGTC 1 cut(s) 820
PshBI ATTAAT 1 cut(s) 602
PspFI CCCAGC 2 cut(s) 233, 560
PspN4I GGNNCC 4 cut(s) 39, 153, 705, 737
PspPI GGNCC 1 cut(s) 257
PstNI CAGNNNCTG 1 cut(s) 458
PsuI RGATCY 1 cut(s) 735
PvuII CAGCTG 2 cut(s) 458, 560
RsaI GTAC 2 cut(s) 204, 372
RsaNI GTAC 2 cut(s) 203, 371
SapI GCTCTTC 1 cut(s) 888
SaqAI TTAA 4 cut(s) 294, 551, 602, 873
SatI GCNGC 6 cut(s) 453, 456, 645, 720, 869, 905
Sau3AI GATC 4 cut(s) 22, 106, 554, 735
Sau96I GGNCC 1 cut(s) 257
SchI GAGTC 1 cut(s) 820
SfaNI GCATC 1 cut(s) 551
SfcI CTRYAG 1 cut(s) 807
SinI GGWCC 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 872
SmoI CTYRAG 1 cut(s) 872
Sse9I AATT 6 cut(s) 57, 288, 507, 637, 840, 878
SsiI CCGC 5 cut(s) 114, 128, 143, 695, 720
SspMI CTAG 1 cut(s) 338
StyI CCWWGG 1 cut(s) 775
TaaI ACNGT 4 cut(s) 207, 370, 808, 853
TaiI ACGT 1 cut(s) 752
TaqI TCGA 1 cut(s) 589
TasI AATT 6 cut(s) 57, 288, 507, 637, 840, 878
TauI GCSGC 1 cut(s) 722
Tru1I TTAA 4 cut(s) 294, 551, 602, 873
Tru9I TTAA 4 cut(s) 294, 551, 602, 873
TscAI CASTG 4 cut(s) 330, 736, 811, 906
TseFI GTSAC 1 cut(s) 384
TseI GCWGC 5 cut(s) 452, 455, 644, 868, 904
Tsp45I GTSAC 1 cut(s) 384
TspDTI ATGAA 3 cut(s) 104, 256, 569
TspGWI ACGGA 2 cut(s) 535, 760
TspRI CASTG 4 cut(s) 330, 736, 811, 906
Vha464I CTTAAG 1 cut(s) 872
VpaK11BI GGWCC 1 cut(s) 257
VspI ATTAAT 1 cut(s) 602
XapI RAATTY 1 cut(s) 507
XcmI CCANNNNNNNNNTGG 1 cut(s) 294
XspI CTAG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.