RLG00000003727

transmembrane protein 64-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0007106
Physical Location & Seq
Forward (+)
52122127 .. 52124892
2766 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003727

Sequence Viewer

Length: 909 bp
ATGACCTATGAGGAGAATGTTGATCGGGGAGAGGTGGAGCCGGAGCTGATCGTCAGAATTGGGGGCGGTGAGGATGATGACAAGGGGAGAGGGGACTATGTGAAGCTCAGGGAGTGTCCCGGCGAGGCTGAGGAGTCGTCGTCATCGTCGTCGTCTTCGCCCAAGGGATTATGGTGTTTGTGGTATTGGGTTAGGTTGGTGTTGCTGTTCATGTGCCTGGGGTTGTTGACCGGGGTTTTTATCAAATGGGTCGGGCCTTTTCTGATGGATACGGAGGTTATTCCAATGATTAATTGGGAAACGAAAACATTCAGTCCTCCAGTGCTTGCTATTCTGGTCTTTGCTTCTGTGGCATTGTTTCCTACCATACTATTACCATCTTCACCTTCTATGTGGGTGGCTGGAATGACTTTTGGTTATGGCTATGGATTTCTTCTCATTATATCAGCAGCATCTATTGGTATATCACTTCCATATTTCATTAGCTATCTCTTCTACCATAAAATTCAGGGATGGTTAGAAAAATATCCAAAGAGAGCATCCATTCTAAGATCAGCTGGCGAAGGAAACTGGTTTCACCAGTTTCGAGCTGTAGCATTAATCAGGATTTCTCCATTCCCTTATATTATATATAACTACTGTGCTGTGGCAACCAATGTCAAGTATGGTCCGTACCTCTTGGGGTCCTTGGCGGGAATGGTTCCAGAAACATTTGTTGCCATTTACACTGGGATCTTAATACGGACATTGGCAGATGCTTCACATGAGCACCACGGCCTTTCAGCCCCACAGATAATCTTCACTGTTGTTGGCTTTGCTTTGGCTGTGGCTACCACAATTTTCTTCACAGTGTATGCAAAAAAGCAGCTCAAGGAATTGCAGAAGGAAGATGAGCCACTGTTGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

303

Amino Acids

33.73

Weight (kDa)

5.78

Isoelectric Point (pI)

43.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 125 - 245 2.2e-15 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 66, 692
AclWI GGATC 1 cut(s) 740
AcsI RAATTY 1 cut(s) 504
AfaI GTAC 1 cut(s) 674
AjnI CCWGG 1 cut(s) 216
AluBI AGCT 6 cut(s) 46, 106, 486, 557, 590, 868
AluI AGCT 6 cut(s) 46, 106, 486, 557, 590, 868
Alw21I GWGCWC 1 cut(s) 771
AlwI GGATC 1 cut(s) 740
AoxI GGCC 2 cut(s) 254, 775
ApeKI GCWGC 2 cut(s) 449, 865
ApoI RAATTY 1 cut(s) 504
AseI ATTAAT 2 cut(s) 291, 599
Asp700I GAANNNNTTC 1 cut(s) 308
AspS9I GGNCC 3 cut(s) 254, 668, 684
AsuC2I CCSGG 2 cut(s) 120, 232
AsuHPI GGTGA 3 cut(s) 80, 375, 569
AvaII GGWCC 2 cut(s) 668, 684
BaeI ACNNNNGTAYC 2 cut(s) 261, 294
BbsI GAAGAC 1 cut(s) 147
Bbv12I GWGCWC 1 cut(s) 771
BbvCI CCTCAGC 1 cut(s) 129
BbvI GCAGC 2 cut(s) 461, 877
BccI CCATC 3 cut(s) 259, 385, 507
BceAI ACGGC 1 cut(s) 790
BciT130I CCWGG 1 cut(s) 218
BciVI GTATCC 1 cut(s) 262
BcnI CCSGG 2 cut(s) 120, 232
BfmI CTRYAG 1 cut(s) 591
BfuI GTATCC 1 cut(s) 262
BisI GCNGC 2 cut(s) 450, 866
BlsI GCNGC 2 cut(s) 451, 867
Bme1390I CCNGG 3 cut(s) 120, 218, 232
Bme18I GGWCC 2 cut(s) 668, 684
BmgT120I GGNCC 3 cut(s) 254, 668, 684
BmiI GGNNCC 3 cut(s) 39, 685, 702
BmrFI CCNGG 3 cut(s) 120, 218, 232
BmrI ACTGGG 1 cut(s) 738
BmsI GCATC 3 cut(s) 461, 548, 745
BmuI ACTGGG 1 cut(s) 738
BpiI GAAGAC 1 cut(s) 147
BpmI CTGGAG 1 cut(s) 303
Bpu10I CCTNAGC 2 cut(s) 107, 129
BpuEI CTTGAG 1 cut(s) 854
BpuMI CCSGG 2 cut(s) 120, 232
BsaJI CCNNGG 5 cut(s) 162, 217, 231, 687, 772
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bse1I ACTGG 4 cut(s) 320, 575, 580, 733
BseBI CCWGG 1 cut(s) 218
BseDI CCNNGG 5 cut(s) 162, 217, 231, 687, 772
BseGI GGATG 3 cut(s) 79, 518, 539
BseMII CTCAG 2 cut(s) 120, 121
BseNI ACTGG 4 cut(s) 320, 575, 580, 733
BseRI GAGGAG 2 cut(s) 26, 146
BseXI GCAGC 2 cut(s) 461, 877
BshFI GGCC 2 cut(s) 256, 777
BsiHKAI GWGCWC 1 cut(s) 771
BsiSI CCGG 3 cut(s) 41, 120, 231
BslFI GGGAC 2 cut(s) 102, 107
BsmFI GGGAC 2 cut(s) 102, 107
BsnI GGCC 2 cut(s) 256, 777
Bsp1286I GDGCHC 1 cut(s) 771
Bsp143I GATC 4 cut(s) 22, 48, 551, 732
BspACI CCGC 2 cut(s) 66, 692
BspANI GGCC 2 cut(s) 256, 777
BspCNI CTCAG 2 cut(s) 120, 121
BspLI GGNNCC 3 cut(s) 39, 685, 702
BspPI GGATC 1 cut(s) 740
BsrI ACTGG 4 cut(s) 320, 575, 580, 733
BssECI CCNNGG 5 cut(s) 162, 217, 231, 687, 772
BssMI GATC 4 cut(s) 22, 48, 551, 732
BssT1I CCWWGG 2 cut(s) 162, 687
Bst2UI CCWGG 1 cut(s) 218
Bst4CI ACNGT 4 cut(s) 641, 805, 850, 900
Bst6I CTCTTC 1 cut(s) 497
BstC8I GCNNGC 2 cut(s) 327, 559
BstDEI CTNAG 3 cut(s) 107, 129, 548
BstDSI CCRYGG 1 cut(s) 772
BstF5I GGATG 3 cut(s) 79, 518, 539
BstKTI GATC 4 cut(s) 25, 51, 554, 735
BstMBI GATC 4 cut(s) 22, 48, 551, 732
BstMWI GCNNNNNNNGC 2 cut(s) 350, 901
BstNI CCWGG 1 cut(s) 218
BstSCI CCNGG 3 cut(s) 118, 216, 230
BstSFI CTRYAG 1 cut(s) 591
BstV1I GCAGC 2 cut(s) 461, 877
BstV2I GAAGAC 1 cut(s) 147
BstX2I RGATCY 1 cut(s) 732
BstYI RGATCY 1 cut(s) 732
BsuI GTATCC 1 cut(s) 262
BsuRI GGCC 2 cut(s) 256, 777
BtgI CCRYGG 1 cut(s) 772
BtsCI GGATG 3 cut(s) 79, 518, 539
BtsIMutI CAGTG 5 cut(s) 327, 726, 801, 855, 896
Cac8I GCNNGC 2 cut(s) 327, 559
Cfr13I GGNCC 3 cut(s) 254, 668, 684
Csp6I GTAC 1 cut(s) 673
CviAII CATG 2 cut(s) 211, 764
CviQI GTAC 1 cut(s) 673
DdeI CTNAG 3 cut(s) 107, 129, 548
DpnI GATC 4 cut(s) 24, 50, 553, 734
DpnII GATC 4 cut(s) 22, 48, 551, 732
Eam1104I CTCTTC 1 cut(s) 497
EarI CTCTTC 1 cut(s) 497
Eco130I CCWWGG 2 cut(s) 162, 687
Eco47I GGWCC 2 cut(s) 668, 684
EcoO109I RGGNCCY 1 cut(s) 684
EcoRII CCWGG 1 cut(s) 216
EcoT14I CCWWGG 2 cut(s) 162, 687
ErhI CCWWGG 2 cut(s) 162, 687
FaeI CATG 2 cut(s) 214, 767
FaqI GGGAC 2 cut(s) 102, 107
FatI CATG 2 cut(s) 210, 763
FauI CCCGC 1 cut(s) 685
Fnu4HI GCNGC 2 cut(s) 450, 866
FokI GGATG 3 cut(s) 86, 525, 526
Fsp4HI GCNGC 2 cut(s) 450, 866
GluI GCNGC 2 cut(s) 450, 866
GsuI CTGGAG 1 cut(s) 303
HaeIII GGCC 2 cut(s) 256, 777
HapII CCGG 3 cut(s) 41, 120, 231
Hin1II CATG 2 cut(s) 214, 767
HincII GTYRAC 1 cut(s) 228
HindII GTYRAC 1 cut(s) 228
HinfI GANTC 1 cut(s) 134
HpaII CCGG 3 cut(s) 41, 120, 231
HphI GGTGA 3 cut(s) 80, 375, 569
Hpy166II GTNNAC 1 cut(s) 228
Hpy188I TCNGA 2 cut(s) 56, 264
Hpy188III TCNNGA 2 cut(s) 604, 704
Hpy8I GTNNAC 1 cut(s) 228
Hpy99I CGWCG 3 cut(s) 142, 151, 154
HpyAV CCTTC 3 cut(s) 396, 557, 877
HpyCH4III ACNGT 4 cut(s) 641, 805, 850, 900
HpyCH4V TGCA 3 cut(s) 857, 880, 904
HpyF10VI GCNNNNNNNGC 2 cut(s) 350, 901
HpyF3I CTNAG 3 cut(s) 107, 129, 548
Hsp92II CATG 2 cut(s) 214, 767
Kzo9I GATC 4 cut(s) 22, 48, 551, 732
LmnI GCTCC 2 cut(s) 37, 43
Lsp1109I GCAGC 2 cut(s) 461, 877
LweI GCATC 3 cut(s) 461, 548, 745
MalI GATC 4 cut(s) 24, 50, 553, 734
MboI GATC 4 cut(s) 22, 48, 551, 732
MboII GAAGA 7 cut(s) 147, 372, 425, 484, 790, 835, 899
MflI RGATCY 1 cut(s) 732
MhlI GDGCHC 1 cut(s) 771
MluCI AATT 5 cut(s) 57, 292, 504, 837, 875
MlyI GAGTC 1 cut(s) 143
MnlI CCTC 9 cut(s) 4, 25, 64, 83, 118, 124, 268, 327, 686
MroXI GAANNNNTTC 1 cut(s) 308
MseI TTAA 3 cut(s) 291, 599, 737
MspA1I CMGCKG 1 cut(s) 557
MspI CCGG 3 cut(s) 41, 120, 231
MspR9I CCNGG 3 cut(s) 120, 218, 232
MvaI CCWGG 1 cut(s) 218
MwoI GCNNNNNNNGC 2 cut(s) 350, 901
NciI CCSGG 2 cut(s) 120, 232
NdeII GATC 4 cut(s) 22, 48, 551, 732
NlaIII CATG 2 cut(s) 214, 767
NlaIV GGNNCC 3 cut(s) 39, 685, 702
PcsI WCGNNNNNNNCGW 2 cut(s) 143, 146
PdmI GAANNNNTTC 1 cut(s) 308
PkrI GCNGC 2 cut(s) 451, 867
PleI GAGTC 1 cut(s) 142
PpsI GAGTC 1 cut(s) 142
PpuMI RGGWCCY 1 cut(s) 684
PshBI ATTAAT 2 cut(s) 291, 599
Psp5II RGGWCCY 1 cut(s) 684
Psp6I CCWGG 1 cut(s) 216
PspGI CCWGG 1 cut(s) 216
PspN4I GGNNCC 3 cut(s) 39, 685, 702
PspPI GGNCC 3 cut(s) 254, 668, 684
PspPPI RGGWCCY 1 cut(s) 684
PsuI RGATCY 1 cut(s) 732
PvuII CAGCTG 1 cut(s) 557
RsaI GTAC 1 cut(s) 674
RsaNI GTAC 1 cut(s) 673
SaqAI TTAA 3 cut(s) 291, 599, 737
SatI GCNGC 2 cut(s) 450, 866
Sau3AI GATC 4 cut(s) 22, 48, 551, 732
Sau96I GGNCC 3 cut(s) 254, 668, 684
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 3 cut(s) 120, 218, 232
SduI GDGCHC 1 cut(s) 771
SfaNI GCATC 3 cut(s) 461, 548, 745
SfcI CTRYAG 1 cut(s) 591
SinI GGWCC 2 cut(s) 668, 684
SmlI CTYRAG 1 cut(s) 869
SmoI CTYRAG 1 cut(s) 869
Sse9I AATT 5 cut(s) 57, 292, 504, 837, 875
SsiI CCGC 2 cut(s) 66, 692
StyD4I CCNGG 3 cut(s) 118, 216, 230
StyI CCWWGG 2 cut(s) 162, 687
TaaI ACNGT 4 cut(s) 641, 805, 850, 900
TaqI TCGA 1 cut(s) 586
TasI AATT 5 cut(s) 57, 292, 504, 837, 875
Tru1I TTAA 3 cut(s) 291, 599, 737
Tru9I TTAA 3 cut(s) 291, 599, 737
TscAI CASTG 5 cut(s) 327, 733, 808, 855, 903
TseI GCWGC 2 cut(s) 449, 865
TspDTI ATGAA 2 cut(s) 199, 469
TspGWI ACGGA 3 cut(s) 287, 660, 757
TspRI CASTG 5 cut(s) 327, 733, 808, 855, 903
VpaK11BI GGWCC 2 cut(s) 668, 684
VspI ATTAAT 2 cut(s) 291, 599
XapI RAATTY 1 cut(s) 504
XcmI CCANNNNNNNNNTGG 1 cut(s) 291
XmnI GAANNNNTTC 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.