MD00G1071900.v1.1

transmembrane protein 64-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0007106
Physical Location & Seq
Forward (+)
14108541 .. 14111967
3427 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1071900.v1.1.491

Sequence Viewer

Length: 912 bp
ATGTCCTATGACGATTTTAAGGATCGGGGAGAGGTGGTGCCGGAGCTGATTTTGAGAGTTGGTGGCGAAGACGACAAAGGGGACTATGCTAAGTTGGTGGATGGTGATCGGGGCGGTTGCGGGACGGCGGAGGGTTTTGAGGCGGTGGCGGAGCCGGCGTCTCCGAGAAAGTTAGCGTCTTTTTGGTATTGGGTTAGGTTGGTATTGTTGGTTATGTGCTTGGGGTTGTTGGCTGGGATTTTTGCAAAATGGGTCGGACCTTATTTCATGGATAAGGAGATTATTCCAATTCTTGACTGGGAGACGAGAACATTCAGTCCTCCAGTGCTTGTGGTTCTAGTCTTTGCTTCTGTGGCATTATTTCCCACTGTACTTTTACCATCTTCACCTTCTATGTGGGTGGCTGGTATGGCATTTGGTTATGGTTATGGATTTCTTCTAATCATATCAGCAGCAGCTGTGGGTGTATCCCTTCCATTCTTCATTGGCTCTCTCTTCTATCACAAAATTCAAGGATGGTTAGAAAAATATCCGAAGAGAGCATCCATTTTAAGATCGGCTGGCGAAGGAAACTGGTTTCATCAGTTTCGAGCTGTAACATTAATCAGGATTTCTCCATTCCCTTATATTATCTATAATTATGCTGCTGTGGCAACAAATGTCAAGTATGTTCCTTACCTGTTGGGGTCCTTGGCGGGAATGGTGCCAGAAACATTTGTCGCCATCTACACTGGGATCCTGATACGGACGTTGGCAGACGCTTCACATGAGCGCCATGGTCTTTCAGCGCCACAGATAATCTTCACTGTAGTTGGCTTCTCTTTGACTGTGGTTACCACAATTTTCTTCACGGTGTATGCAAAAAGGCAGCTCAAGGAATTACAAAATGACGACGAGCCACTGCTGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

304

Amino Acids

33.62

Weight (kDa)

6.11

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 126 - 246 1.5e-16 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 37, 703
AciI CCGC 6 cut(s) 114, 120, 128, 143, 149, 695
AclWI GGATC 3 cut(s) 30, 730, 743
AcsI RAATTY 1 cut(s) 507
AcyI GRCGYC 1 cut(s) 158
AfaI GTAC 1 cut(s) 372
AgsI TTSAA 1 cut(s) 512
AloI GAACNNNNNNTCC 2 cut(s) 301, 333
AluBI AGCT 4 cut(s) 46, 458, 593, 871
AluI AGCT 4 cut(s) 46, 458, 593, 871
Alw26I GTCTC 2 cut(s) 165, 296
AlwI GGATC 3 cut(s) 30, 730, 743
AlwNI CAGNNNCTG 1 cut(s) 458
ApeKI GCWGC 5 cut(s) 452, 455, 644, 868, 904
ApoI RAATTY 1 cut(s) 507
AseI ATTAAT 1 cut(s) 602
AspLEI GCGC 2 cut(s) 774, 790
AspS9I GGNCC 2 cut(s) 257, 687
AsuHPI GGTGA 2 cut(s) 116, 378
AvaII GGWCC 2 cut(s) 257, 687
BaeI ACNNNNGTAYC 2 cut(s) 734, 767
BamHI GGATCC 1 cut(s) 735
BanI GGYRCC 2 cut(s) 37, 703
BbsI GAAGAC 1 cut(s) 75
BbvI GCAGC 5 cut(s) 464, 467, 631, 880, 891
BccI CCATC 4 cut(s) 95, 388, 510, 731
BceAI ACGGC 1 cut(s) 141
BciVI GTATCC 1 cut(s) 478
BcoDI GTCTC 2 cut(s) 165, 296
BfaI CTAG 1 cut(s) 338
BfmI CTRYAG 1 cut(s) 807
BfoI RGCGCY 2 cut(s) 775, 791
BfuI GTATCC 1 cut(s) 478
BisI GCNGC 5 cut(s) 453, 456, 645, 869, 905
BlsI GCNGC 5 cut(s) 454, 457, 646, 870, 906
Bme18I GGWCC 2 cut(s) 257, 687
BmgT120I GGNCC 2 cut(s) 257, 687
BmiI GGNNCC 5 cut(s) 39, 153, 688, 705, 737
BmrI ACTGGG 2 cut(s) 307, 741
BmsI GCATC 1 cut(s) 551
BmuI ACTGGG 2 cut(s) 307, 741
BpiI GAAGAC 1 cut(s) 75
BpmI CTGGAG 1 cut(s) 306
BpuEI CTTGAG 1 cut(s) 857
BsaBI GATNNNNATC 1 cut(s) 105
BsaHI GRCGYC 1 cut(s) 158
BsaJI CCNNGG 2 cut(s) 690, 775
BsaXI ACNNNNNCTCC 4 cut(s) 21, 51, 143, 173
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 4 cut(s) 302, 323, 578, 736
Bse8I GATNNNNATC 1 cut(s) 105
BseDI CCNNGG 2 cut(s) 690, 775
BseGI GGATG 3 cut(s) 106, 521, 542
BseJI GATNNNNATC 1 cut(s) 105
BseNI ACTGG 4 cut(s) 302, 323, 578, 736
BseXI GCAGC 5 cut(s) 464, 467, 631, 880, 891
BseYI CCCAGC 1 cut(s) 233
BshNI GGYRCC 2 cut(s) 37, 703
BsiSI CCGG 2 cut(s) 41, 155
BslFI GGGAC 2 cut(s) 95, 136
BsmAI GTCTC 2 cut(s) 165, 296
BsmBI CGTCTC 2 cut(s) 165, 296
BsmFI GGGAC 2 cut(s) 95, 136
Bsp143I GATC 4 cut(s) 22, 106, 554, 735
Bsp19I CCATGG 1 cut(s) 775
BspACI CCGC 6 cut(s) 114, 120, 128, 143, 149, 695
BspLI GGNNCC 5 cut(s) 39, 153, 688, 705, 737
BspPI GGATC 3 cut(s) 30, 730, 743
BspT107I GGYRCC 2 cut(s) 37, 703
BsrFI RCCGGY 1 cut(s) 154
BsrI ACTGG 4 cut(s) 302, 323, 578, 736
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 2 cut(s) 690, 775
BssMI GATC 4 cut(s) 22, 106, 554, 735
BssNI GRCGYC 1 cut(s) 158
BssT1I CCWWGG 2 cut(s) 690, 775
Bst4CI ACNGT 4 cut(s) 370, 808, 829, 853
Bst6I CTCTTC 2 cut(s) 500, 530
BstACI GRCGYC 1 cut(s) 158
BstC8I GCNNGC 2 cut(s) 156, 562
BstDEI CTNAG 1 cut(s) 90
BstDSI CCRYGG 1 cut(s) 775
BstEII GGTNACC 1 cut(s) 832
BstF5I GGATG 3 cut(s) 106, 521, 542
BstH2I RGCGCY 2 cut(s) 775, 791
BstHHI GCGC 2 cut(s) 774, 790
BstKTI GATC 4 cut(s) 25, 109, 557, 738
BstMAI GTCTC 2 cut(s) 165, 296
BstMBI GATC 4 cut(s) 22, 106, 554, 735
BstMWI GCNNNNNNNGC 5 cut(s) 155, 353, 410, 650, 904
BstPI GGTNACC 1 cut(s) 832
BstSFI CTRYAG 1 cut(s) 807
BstV1I GCAGC 5 cut(s) 464, 467, 631, 880, 891
BstV2I GAAGAC 1 cut(s) 75
BstX2I RGATCY 1 cut(s) 735
BstYI RGATCY 1 cut(s) 735
BsuI GTATCC 1 cut(s) 478
BtgI CCRYGG 1 cut(s) 775
BtsCI GGATG 3 cut(s) 106, 521, 542
BtsI GCAGTG 1 cut(s) 899
BtsIMutI CAGTG 5 cut(s) 330, 366, 729, 804, 899
Cac8I GCNNGC 2 cut(s) 156, 562
CaiI CAGNNNCTG 1 cut(s) 458
CfoI GCGC 2 cut(s) 774, 790
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 2 cut(s) 257, 687
CseI GACGC 3 cut(s) 147, 165, 767
Csp6I GTAC 1 cut(s) 371
CviAII CATG 3 cut(s) 268, 767, 776
CviQI GTAC 1 cut(s) 371
DdeI CTNAG 1 cut(s) 90
DpnI GATC 4 cut(s) 24, 108, 556, 737
DpnII GATC 4 cut(s) 22, 106, 554, 735
Eam1104I CTCTTC 2 cut(s) 500, 530
EarI CTCTTC 2 cut(s) 500, 530
EciI GGCGGA 2 cut(s) 143, 164
Eco130I CCWWGG 2 cut(s) 690, 775
Eco47I GGWCC 2 cut(s) 257, 687
Eco91I GGTNACC 1 cut(s) 832
EcoO109I RGGNCCY 1 cut(s) 687
EcoO65I GGTNACC 1 cut(s) 832
EcoT14I CCWWGG 2 cut(s) 690, 775
ErhI CCWWGG 2 cut(s) 690, 775
Esp3I CGTCTC 2 cut(s) 165, 296
FaeI CATG 3 cut(s) 271, 770, 779
FaqI GGGAC 2 cut(s) 95, 136
FatI CATG 3 cut(s) 267, 766, 775
FauI CCCGC 2 cut(s) 113, 688
Fnu4HI GCNGC 5 cut(s) 453, 456, 645, 869, 905
FokI GGATG 3 cut(s) 113, 528, 529
Fsp4HI GCNGC 5 cut(s) 453, 456, 645, 869, 905
FspBI CTAG 1 cut(s) 338
GlaI GCGC 2 cut(s) 773, 789
GluI GCNGC 5 cut(s) 453, 456, 645, 869, 905
GsaI CCCAGC 1 cut(s) 237
GsuI CTGGAG 1 cut(s) 306
HaeII RGCGCY 2 cut(s) 775, 791
HapII CCGG 2 cut(s) 41, 155
HgaI GACGC 3 cut(s) 147, 165, 767
HhaI GCGC 2 cut(s) 774, 790
Hin1I GRCGYC 1 cut(s) 158
Hin1II CATG 3 cut(s) 271, 770, 779
Hin6I GCGC 2 cut(s) 772, 788
HinP1I GCGC 2 cut(s) 772, 788
HpaII CCGG 2 cut(s) 41, 155
HphI GGTGA 2 cut(s) 116, 378
Hpy188I TCNGA 3 cut(s) 165, 257, 534
Hpy188III TCNNGA 3 cut(s) 293, 607, 739
Hpy99I CGWCG 1 cut(s) 896
HpyAV CCTTC 3 cut(s) 399, 482, 560
HpyCH4III ACNGT 4 cut(s) 370, 808, 829, 853
HpyCH4IV ACGT 1 cut(s) 749
HpyCH4V TGCA 3 cut(s) 245, 860, 907
HpyF10VI GCNNNNNNNGC 5 cut(s) 155, 353, 410, 650, 904
HpyF3I CTNAG 1 cut(s) 90
HpySE526I ACGT 1 cut(s) 749
Hsp92I GRCGYC 1 cut(s) 158
Hsp92II CATG 3 cut(s) 271, 770, 779
HspAI GCGC 2 cut(s) 772, 788
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
Kzo9I GATC 4 cut(s) 22, 106, 554, 735
LmnI GCTCC 2 cut(s) 43, 151
Lsp1109I GCAGC 5 cut(s) 464, 467, 631, 880, 891
LweI GCATC 1 cut(s) 551
MaeI CTAG 1 cut(s) 338
MaeII ACGT 1 cut(s) 749
MaeIII GTNAC 2 cut(s) 595, 832
MalI GATC 4 cut(s) 24, 108, 556, 737
MboI GATC 4 cut(s) 22, 106, 554, 735
MboII GAAGA 8 cut(s) 80, 375, 428, 472, 487, 547, 793, 838
MflI RGATCY 1 cut(s) 735
MluCI AATT 5 cut(s) 288, 507, 637, 840, 878
MmeI TCCRAC 1 cut(s) 235
MnlI CCTC 4 cut(s) 25, 124, 133, 330
MroNI GCCGGC 1 cut(s) 154
MseI TTAA 3 cut(s) 18, 551, 602
MspA1I CMGCKG 1 cut(s) 458
MspI CCGG 2 cut(s) 41, 155
MwoI GCNNNNNNNGC 5 cut(s) 155, 353, 410, 650, 904
NaeI GCCGGC 1 cut(s) 156
NcoI CCATGG 1 cut(s) 775
NdeII GATC 4 cut(s) 22, 106, 554, 735
NgoMIV GCCGGC 1 cut(s) 154
NlaIII CATG 3 cut(s) 271, 770, 779
NlaIV GGNNCC 5 cut(s) 39, 153, 688, 705, 737
PdiI GCCGGC 1 cut(s) 156
PkrI GCNGC 5 cut(s) 454, 457, 646, 870, 906
PpuMI RGGWCCY 1 cut(s) 687
PshBI ATTAAT 1 cut(s) 602
Psp5II RGGWCCY 1 cut(s) 687
PspEI GGTNACC 1 cut(s) 832
PspFI CCCAGC 1 cut(s) 233
PspN4I GGNNCC 5 cut(s) 39, 153, 688, 705, 737
PspPI GGNCC 2 cut(s) 257, 687
PspPPI RGGWCCY 1 cut(s) 687
PstNI CAGNNNCTG 1 cut(s) 458
PsuI RGATCY 1 cut(s) 735
PvuII CAGCTG 1 cut(s) 458
RsaI GTAC 1 cut(s) 372
RsaNI GTAC 1 cut(s) 371
SaqAI TTAA 3 cut(s) 18, 551, 602
SatI GCNGC 5 cut(s) 453, 456, 645, 869, 905
Sau3AI GATC 4 cut(s) 22, 106, 554, 735
Sau96I GGNCC 2 cut(s) 257, 687
SfaNI GCATC 1 cut(s) 551
SfcI CTRYAG 1 cut(s) 807
SinI GGWCC 2 cut(s) 257, 687
SmlI CTYRAG 1 cut(s) 872
SmoI CTYRAG 1 cut(s) 872
Sse9I AATT 5 cut(s) 288, 507, 637, 840, 878
SsiI CCGC 6 cut(s) 114, 120, 128, 143, 149, 695
SspMI CTAG 1 cut(s) 338
StyI CCWWGG 2 cut(s) 690, 775
TaaI ACNGT 4 cut(s) 370, 808, 829, 853
TaiI ACGT 1 cut(s) 752
TaqI TCGA 1 cut(s) 589
TasI AATT 5 cut(s) 288, 507, 637, 840, 878
TatI WGTACW 1 cut(s) 370
Tru1I TTAA 3 cut(s) 18, 551, 602
Tru9I TTAA 3 cut(s) 18, 551, 602
TscAI CASTG 5 cut(s) 330, 373, 736, 811, 906
TseI GCWGC 5 cut(s) 452, 455, 644, 868, 904
TspDTI ATGAA 3 cut(s) 256, 472, 569
TspGWI ACGGA 1 cut(s) 760
TspRI CASTG 5 cut(s) 330, 373, 736, 811, 906
VpaK11BI GGWCC 2 cut(s) 257, 687
VspI ATTAAT 1 cut(s) 602
XapI RAATTY 1 cut(s) 507
XcmI CCANNNNNNNNNTGG 1 cut(s) 294
XspI CTAG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.