RchiOBHm_Chr7g0200421

transmembrane protein 64-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0007106
Physical Location & Seq
Reverse (-)
18201209 .. 18205086
3878 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17939

Sequence Viewer

Length: 906 bp
ATGACCTATGAGGAGAATGTTGATCGAGGAGAGGTGGAGCCGGAGCTGATCGTGAGAATTGGGGGCGGTGACGATGATGAGAAGGGGAGAGGGGACTATGTGAAGCTCAGGGAGTGTCCCGGCGAGGCTGAGGAGTCGGAGCCGTCGTCGTCTTCGCCCAAGGGATTATGGTGTTTGTGGTATTGGGTTAGGTTGGTGTTGCTGTTCATGTGCTTGGGGTTGTTGGCTGGGGTTTTTATCAAATGGGTCGGGCCTTTTCTGATGGATACGGAGGTAATTCCAATGATTAACTGGGAGACAAAAACATTCAGTCCTCCAGTGCTTGCTATTCTGGTCTTTGCTTCTGTGGCATTGTTTCCTACCATACTATTACCATCTTCACCTTCTATGTGGGTGGCTGGAATGACTTTTGGTTATGGCTATGGATTTCTTCTCATTATATCAGCAGCAGCTATTGGTATATCACTTCCATATTTCATTAGCTATCTCTTCTACCATAAAATTCAGGGATGGTTAGAAAAATATCCAAAGAGAGCTTCCATTCTAAGATCAGCTGGCGAAGGAAACTGGTTTCACCAGTTTCGAGCTGTAGCATTAATCAGGATTTCTCCATTCCCTTATATTATATATAACTACTGTGCTGTGGCAACCAATGTCAAGTATGGTCCGTACCTCTTGGGGTCCTTGGCGGGAATGGTTCCAGAAGCATTTGTTGCCATTTACACTGGGATCTTAATACGGACATTGGCAGATGCTTCACATGAGCACCACGGCCTTTCAGCCCCACAGATAATCTTCACTGTTGTTGGCTTTGCTTTGGCTGTGGCTACCACAATTTTCTTCACAGTGTATGCAAAAAAGCAGCTCAAGGAATTGCAGAAGGAAGATGAGCCACTGTTGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

301

Amino Acids

33.62

Weight (kDa)

5.61

Isoelectric Point (pI)

42.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 124 - 244 2.5e-15 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 66, 689
AclWI GGATC 1 cut(s) 737
AcsI RAATTY 1 cut(s) 501
AfaI GTAC 1 cut(s) 671
AjuI GAANNNNNNNTTGG 2 cut(s) 520, 552
AluBI AGCT 8 cut(s) 46, 106, 452, 483, 536, 554, 587, 865
AluI AGCT 8 cut(s) 46, 106, 452, 483, 536, 554, 587, 865
Alw21I GWGCWC 1 cut(s) 768
Alw26I GTCTC 1 cut(s) 290
AlwI GGATC 1 cut(s) 737
AoxI GGCC 2 cut(s) 251, 772
ApeKI GCWGC 3 cut(s) 446, 449, 862
ApoI RAATTY 1 cut(s) 501
AseI ATTAAT 1 cut(s) 596
AspS9I GGNCC 3 cut(s) 251, 665, 681
AsuC2I CCSGG 1 cut(s) 120
AsuHPI GGTGA 3 cut(s) 80, 372, 566
AvaII GGWCC 2 cut(s) 665, 681
BaeI ACNNNNGTAYC 2 cut(s) 258, 291
BbsI GAAGAC 1 cut(s) 144
Bbv12I GWGCWC 1 cut(s) 768
BbvCI CCTCAGC 1 cut(s) 129
BbvI GCAGC 3 cut(s) 458, 461, 874
BccI CCATC 3 cut(s) 256, 382, 504
BceAI ACGGC 2 cut(s) 127, 787
BciVI GTATCC 1 cut(s) 259
BcnI CCSGG 1 cut(s) 120
BcoDI GTCTC 1 cut(s) 290
BfmI CTRYAG 1 cut(s) 588
BfuI GTATCC 1 cut(s) 259
BisI GCNGC 3 cut(s) 447, 450, 863
BlsI GCNGC 3 cut(s) 448, 451, 864
Bme1390I CCNGG 1 cut(s) 120
Bme18I GGWCC 2 cut(s) 665, 681
BmgT120I GGNCC 3 cut(s) 251, 665, 681
BmiI GGNNCC 4 cut(s) 39, 141, 682, 699
BmrFI CCNGG 1 cut(s) 120
BmrI ACTGGG 2 cut(s) 301, 735
BmsI GCATC 1 cut(s) 742
BmuI ACTGGG 2 cut(s) 301, 735
BpiI GAAGAC 1 cut(s) 144
BpmI CTGGAG 1 cut(s) 300
Bpu10I CCTNAGC 2 cut(s) 107, 129
BpuEI CTTGAG 1 cut(s) 851
BpuMI CCSGG 1 cut(s) 120
BsaJI CCNNGG 3 cut(s) 159, 684, 769
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bse1I ACTGG 5 cut(s) 296, 317, 572, 577, 730
BseDI CCNNGG 3 cut(s) 159, 684, 769
BseGI GGATG 1 cut(s) 515
BseMII CTCAG 2 cut(s) 120, 121
BseNI ACTGG 5 cut(s) 296, 317, 572, 577, 730
BseRI GAGGAG 3 cut(s) 26, 42, 146
BseXI GCAGC 3 cut(s) 458, 461, 874
BseYI CCCAGC 1 cut(s) 227
BshFI GGCC 2 cut(s) 253, 774
BsiHKAI GWGCWC 1 cut(s) 768
BsiSI CCGG 2 cut(s) 41, 120
BslFI GGGAC 2 cut(s) 102, 107
BsmAI GTCTC 1 cut(s) 290
BsmFI GGGAC 2 cut(s) 102, 107
BsnI GGCC 2 cut(s) 253, 774
Bsp1286I GDGCHC 1 cut(s) 768
Bsp143I GATC 4 cut(s) 22, 48, 548, 729
BspACI CCGC 2 cut(s) 66, 689
BspANI GGCC 2 cut(s) 253, 774
BspCNI CTCAG 2 cut(s) 120, 121
BspLI GGNNCC 4 cut(s) 39, 141, 682, 699
BspPI GGATC 1 cut(s) 737
BsrI ACTGG 5 cut(s) 296, 317, 572, 577, 730
BssECI CCNNGG 3 cut(s) 159, 684, 769
BssMI GATC 4 cut(s) 22, 48, 548, 729
BssT1I CCWWGG 2 cut(s) 159, 684
Bst4CI ACNGT 4 cut(s) 638, 802, 847, 897
Bst6I CTCTTC 1 cut(s) 494
BstAPI GCANNNNNTGC 1 cut(s) 713
BstC8I GCNNGC 2 cut(s) 324, 556
BstDEI CTNAG 3 cut(s) 107, 129, 545
BstDSI CCRYGG 1 cut(s) 769
BstF5I GGATG 1 cut(s) 515
BstKTI GATC 4 cut(s) 25, 51, 551, 732
BstMAI GTCTC 1 cut(s) 290
BstMBI GATC 4 cut(s) 22, 48, 548, 729
BstMWI GCNNNNNNNGC 3 cut(s) 347, 713, 898
BstSCI CCNGG 1 cut(s) 118
BstSFI CTRYAG 1 cut(s) 588
BstV1I GCAGC 3 cut(s) 458, 461, 874
BstV2I GAAGAC 1 cut(s) 144
BstX2I RGATCY 1 cut(s) 729
BstYI RGATCY 1 cut(s) 729
BsuI GTATCC 1 cut(s) 259
BsuRI GGCC 2 cut(s) 253, 774
BtgI CCRYGG 1 cut(s) 769
BtsCI GGATG 1 cut(s) 515
BtsIMutI CAGTG 5 cut(s) 324, 723, 798, 852, 893
Cac8I GCNNGC 2 cut(s) 324, 556
Cfr13I GGNCC 3 cut(s) 251, 665, 681
Csp6I GTAC 1 cut(s) 670
CviAII CATG 2 cut(s) 208, 761
CviQI GTAC 1 cut(s) 670
DdeI CTNAG 3 cut(s) 107, 129, 545
DpnI GATC 4 cut(s) 24, 50, 550, 731
DpnII GATC 4 cut(s) 22, 48, 548, 729
Eam1104I CTCTTC 1 cut(s) 494
EarI CTCTTC 1 cut(s) 494
Eco130I CCWWGG 2 cut(s) 159, 684
Eco47I GGWCC 2 cut(s) 665, 681
EcoO109I RGGNCCY 1 cut(s) 681
EcoT14I CCWWGG 2 cut(s) 159, 684
ErhI CCWWGG 2 cut(s) 159, 684
FaeI CATG 2 cut(s) 211, 764
FaqI GGGAC 2 cut(s) 102, 107
FatI CATG 2 cut(s) 207, 760
FauI CCCGC 1 cut(s) 682
Fnu4HI GCNGC 3 cut(s) 447, 450, 863
FokI GGATG 1 cut(s) 522
Fsp4HI GCNGC 3 cut(s) 447, 450, 863
GluI GCNGC 3 cut(s) 447, 450, 863
GsaI CCCAGC 1 cut(s) 231
GsuI CTGGAG 1 cut(s) 300
HaeIII GGCC 2 cut(s) 253, 774
HapII CCGG 2 cut(s) 41, 120
Hin1II CATG 2 cut(s) 211, 764
HinfI GANTC 1 cut(s) 134
HpaII CCGG 2 cut(s) 41, 120
HphI GGTGA 3 cut(s) 80, 372, 566
Hpy188I TCNGA 2 cut(s) 139, 261
Hpy188III TCNNGA 3 cut(s) 52, 601, 701
Hpy99I CGWCG 2 cut(s) 148, 151
HpyAV CCTTC 4 cut(s) 76, 393, 554, 874
HpyCH4III ACNGT 4 cut(s) 638, 802, 847, 897
HpyCH4V TGCA 3 cut(s) 854, 877, 901
HpyF10VI GCNNNNNNNGC 3 cut(s) 347, 713, 898
HpyF3I CTNAG 3 cut(s) 107, 129, 545
Hsp92II CATG 2 cut(s) 211, 764
Kzo9I GATC 4 cut(s) 22, 48, 548, 729
LmnI GCTCC 3 cut(s) 37, 43, 139
Lsp1109I GCAGC 3 cut(s) 458, 461, 874
LweI GCATC 1 cut(s) 742
MaeIII GTNAC 1 cut(s) 68
MalI GATC 4 cut(s) 24, 50, 550, 731
MboI GATC 4 cut(s) 22, 48, 548, 729
MboII GAAGA 7 cut(s) 144, 369, 422, 481, 787, 832, 896
MflI RGATCY 1 cut(s) 729
MhlI GDGCHC 1 cut(s) 768
MluCI AATT 5 cut(s) 57, 276, 501, 834, 872
MlyI GAGTC 1 cut(s) 143
MmeI TCCRAC 1 cut(s) 117
MnlI CCTC 9 cut(s) 4, 20, 25, 83, 118, 124, 265, 324, 683
MseI TTAA 3 cut(s) 288, 596, 734
MspA1I CMGCKG 1 cut(s) 554
MspI CCGG 2 cut(s) 41, 120
MspR9I CCNGG 1 cut(s) 120
MwoI GCNNNNNNNGC 3 cut(s) 347, 713, 898
NciI CCSGG 1 cut(s) 120
NdeII GATC 4 cut(s) 22, 48, 548, 729
NlaIII CATG 2 cut(s) 211, 764
NlaIV GGNNCC 4 cut(s) 39, 141, 682, 699
NmuCI GTSAC 1 cut(s) 68
PcsI WCGNNNNNNNCGW 1 cut(s) 143
PkrI GCNGC 3 cut(s) 448, 451, 864
PleI GAGTC 1 cut(s) 142
PpsI GAGTC 1 cut(s) 142
PpuMI RGGWCCY 1 cut(s) 681
PshBI ATTAAT 1 cut(s) 596
Psp5II RGGWCCY 1 cut(s) 681
PspFI CCCAGC 1 cut(s) 227
PspN4I GGNNCC 4 cut(s) 39, 141, 682, 699
PspPI GGNCC 3 cut(s) 251, 665, 681
PspPPI RGGWCCY 1 cut(s) 681
PsuI RGATCY 1 cut(s) 729
PvuII CAGCTG 1 cut(s) 554
RsaI GTAC 1 cut(s) 671
RsaNI GTAC 1 cut(s) 670
SaqAI TTAA 3 cut(s) 288, 596, 734
SatI GCNGC 3 cut(s) 447, 450, 863
Sau3AI GATC 4 cut(s) 22, 48, 548, 729
Sau96I GGNCC 3 cut(s) 251, 665, 681
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 1 cut(s) 120
SduI GDGCHC 1 cut(s) 768
SfaNI GCATC 1 cut(s) 742
SfcI CTRYAG 1 cut(s) 588
SinI GGWCC 2 cut(s) 665, 681
SmlI CTYRAG 1 cut(s) 866
SmoI CTYRAG 1 cut(s) 866
Sse9I AATT 5 cut(s) 57, 276, 501, 834, 872
SsiI CCGC 2 cut(s) 66, 689
StyD4I CCNGG 1 cut(s) 118
StyI CCWWGG 2 cut(s) 159, 684
TaaI ACNGT 4 cut(s) 638, 802, 847, 897
TaqI TCGA 2 cut(s) 25, 583
TasI AATT 5 cut(s) 57, 276, 501, 834, 872
Tru1I TTAA 3 cut(s) 288, 596, 734
Tru9I TTAA 3 cut(s) 288, 596, 734
TscAI CASTG 5 cut(s) 324, 730, 805, 852, 900
TseFI GTSAC 1 cut(s) 68
TseI GCWGC 3 cut(s) 446, 449, 862
Tsp45I GTSAC 1 cut(s) 68
TspDTI ATGAA 2 cut(s) 196, 466
TspGWI ACGGA 3 cut(s) 284, 657, 754
TspRI CASTG 5 cut(s) 324, 730, 805, 852, 900
VpaK11BI GGWCC 2 cut(s) 665, 681
VspI ATTAAT 1 cut(s) 596
XapI RAATTY 1 cut(s) 501
XcmI CCANNNNNNNNNTGG 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.