AT1G16250

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010405
Physical Location & Seq
Forward (+)
5556964 .. 5559520
2557 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G16250.1

Sequence Viewer

Length: 1152 bp
ATGGAATCAATCGAGCAGTCTATAATCCCAGGTTTGCCTGATGATTTGGCTTTGAGATGCATAGCGAAGCTTTCTCATGGGTATCATGGAGTGCTCGAATGTGTTTCCAGAGGCTGGAGAGATTTAGTTCGTGGTGCTGATTACTCGTGCTATAAAGCTAGAAATGGATGGTCAGGGAGCTGGTTGTTTGTGTTGACGGAACGTTCTAAGAATCAGTGGGTTGCTTATGATCCTGAAGCTGATCGATGGCATCCATTGCCTAGAACTAGAGCTGTTCAAGATGGTTGGCATCATTCTGGTTTTGCGTGTGTTTGTGTTTCCAATTGTCTTCTTGTGATTGGGGGTTGTTATGCGCCTTCGGTGTCATCGTTTCCTCATCAGAAGCCTGTTGTCACTAAAGATGTCATGCGGTTTGATCCGTTTAAGAAACAATGGAAAATGGTTGCTAGTATGCGAACACCACGGACTCACTTTGCTTGTACTTCTGTCTCTGGCAAGGTTTATGTTGCTGGAGGTCGTAATTTAACTCATTCCAGGGGAATCCCATCTGCTGAGGTTTATGATCCTGTCGCTGATAGGTGGGAGGAATTACCAGCAATGCCTAGACCACAGATGGACTGCTCGGGGTTATCGTACAGAGGCTGCTTTCATGTTTTAAGTGACCAGGTGGGATTTGCAGAGCAAAATTCATCTGAAGTTTTTAATCCACGAGACATGACTTGGTCCACTGTGGAGGATGTCTGGCCTTTCTCTAGAGCCATGCAGTTTGCTGTTCAGGTGATGAAAAATGATCGGGTGTATACTATTGTAGATTGGGGAGAAAGCTTGATCAAGACTAGGGATACAGATGAAGGGGAATGGTACAATGTTGGTTCAGTTCCTTCTGTTGTTCTTCCTAATCATCCGAGAGAGTTAGAAGCCTTCGGTTATGGGTTTGCAGCTCTAAGGAATGAACTCTATGTTATAGGAGGTAAGGTCCTTAAATGGGAAGAATCAGGGGCAGGGAGATTTGACATTGTGAGATTGCCTGTTGTGAGGGTATGTAACCCTTTGGATAGACCTCTGAATTGGAGAGAGACCAAACCAATGTGCATTCCAGCCGGTGGGTCGATCATTGGTTGTGTATCCTTGGAAGAATCTTCTCCCCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

383

Amino Acids

43.07

Weight (kDa)

6.79

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 69 - 267 1.1e-09 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 71 - 199 1.8e-07 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 99 - 152 3e-06 Kelch motif
Beta-prop_FBX42 PF13415 99 - 195 3.3e-06 FBX42, beta-propeller domain
Kelch_FKB95 PF25210 104 - 293 3e-14 FKB95, Kelch-repeats domain
Kelch_1 PF01344 154 - 201 3.1e-11 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16250 AT1G16250
fragaria_vesca FvH4_4g21831 FvH4_4g21831 FvH4_4g21831 FvH4_4g21831
malus_domestica MD13G1136500.v1.1 MD16G1131800.v1.1
prunus_persica Prupe.1G209000_v2.0.a1
pyrus_communis pycom13g11890 pycom16g11620
rosa_chinensis RchiOBHm_Chr4g0428071
rosa_laevigata RLG00000007184
rosa_multiflora Rmu_sc0005263.1_g000001
rosa_roxburghii Rroxscaffold_5G00369790
rosa_rugosa Rorug04G0222100
rosa_samantha Rh4AG279300 Rh4BG284700 Rh4CG300400 Rh4DG281800
rosa_wichuraiana Rw4G024270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 114, 1103
AccI GTMKAC 1 cut(s) 800
AciI CCGC 1 cut(s) 409
AclI AACGTT 1 cut(s) 202
AclWI GGATC 3 cut(s) 224, 410, 557
AcsI RAATTY 1 cut(s) 685
AcuI CTGAAG 2 cut(s) 255, 714
AfaI GTAC 3 cut(s) 481, 635, 863
AfiI CCNNNNNNNGG 3 cut(s) 114, 985, 1103
AgsI TTSAA 1 cut(s) 278
AjnI CCWGG 3 cut(s) 28, 533, 663
AluBI AGCT 7 cut(s) 70, 158, 180, 239, 272, 825, 941
AluI AGCT 7 cut(s) 70, 158, 180, 239, 272, 825, 941
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 3 cut(s) 493, 705, 1070
AlwI GGATC 3 cut(s) 224, 410, 557
AlwNI CAGNNNCTG 2 cut(s) 114, 642
Ama87I CYCGRG 1 cut(s) 622
AoxI GGCC 1 cut(s) 743
ApeKI GCWGC 2 cut(s) 642, 938
ApoI RAATTY 1 cut(s) 685
AspLEI GCGC 1 cut(s) 355
AspS9I GGNCC 2 cut(s) 723, 976
AsuHPI GGTGA 1 cut(s) 790
AvaI CYCGRG 1 cut(s) 622
AvaII GGWCC 2 cut(s) 723, 976
BauI CACGAG 2 cut(s) 145, 708
BbsI GAAGAC 1 cut(s) 320
Bbv12I GWGCWC 1 cut(s) 96
BbvCI CCTCAGC 1 cut(s) 552
BbvI GCAGC 2 cut(s) 629, 950
BccI CCATC 5 cut(s) 162, 240, 275, 553, 607
BciT130I CCWGG 3 cut(s) 30, 535, 665
BciVI GTATCC 2 cut(s) 835, 1135
BclI TGATCA 1 cut(s) 828
BcoDI GTCTC 3 cut(s) 493, 705, 1070
BfaI CTAG 7 cut(s) 159, 261, 267, 447, 603, 753, 837
BfuI GTATCC 2 cut(s) 835, 1135
BisI GCNGC 2 cut(s) 643, 939
BlsI GCNGC 2 cut(s) 644, 940
Bme1390I CCNGG 3 cut(s) 30, 535, 665
Bme18I GGWCC 2 cut(s) 723, 976
BmeT110I CYCGRG 1 cut(s) 622
BmgT120I GGNCC 2 cut(s) 723, 976
BmrFI CCNGG 3 cut(s) 30, 535, 665
BmsI GCATC 3 cut(s) 47, 259, 298
BpiI GAAGAC 1 cut(s) 320
BpmI CTGGAG 2 cut(s) 136, 531
Bpu10I CCTNAGC 1 cut(s) 552
Bsa29I ATCGAT 1 cut(s) 244
BsaI GGTCTC 1 cut(s) 1070
BsaJI CCNNGG 4 cut(s) 28, 461, 534, 1128
BsaXI ACNNNNNCTCC 2 cut(s) 960, 990
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 985, 1103
Bse118I RCCGGY 1 cut(s) 1100
Bse3DI GCAATG 2 cut(s) 254, 603
BseBI CCWGG 3 cut(s) 30, 535, 665
BseCI ATCGAT 1 cut(s) 244
BseDI CCNNGG 4 cut(s) 28, 461, 534, 1128
BseGI GGATG 4 cut(s) 173, 250, 742, 901
BseLI CCNNNNNNNGG 3 cut(s) 114, 985, 1103
BseMI GCAATG 2 cut(s) 254, 603
BseMII CTCAG 1 cut(s) 543
BseXI GCAGC 2 cut(s) 629, 950
BshFI GGCC 1 cut(s) 745
BshVI ATCGAT 1 cut(s) 244
BsiHKAI GWGCWC 1 cut(s) 96
BsiHKCI CYCGRG 1 cut(s) 622
BsiSI CCGG 1 cut(s) 1101
BslI CCNNNNNNNGG 3 cut(s) 114, 985, 1103
BsmAI GTCTC 3 cut(s) 493, 705, 1070
BsmI GAATGC 1 cut(s) 1092
BsnI GGCC 1 cut(s) 745
Bso31I GGTCTC 1 cut(s) 1070
BsoBI CYCGRG 1 cut(s) 622
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 7 cut(s) 229, 241, 415, 562, 790, 828, 1110
BspACI CCGC 1 cut(s) 409
BspANI GGCC 1 cut(s) 745
BspCNI CTCAG 1 cut(s) 544
BspDI ATCGAT 1 cut(s) 244
BspPI GGATC 3 cut(s) 224, 410, 557
BspTNI GGTCTC 1 cut(s) 1070
BsrDI GCAATG 2 cut(s) 254, 603
BsrFI RCCGGY 1 cut(s) 1100
BssAI RCCGGY 1 cut(s) 1100
BssECI CCNNGG 4 cut(s) 28, 461, 534, 1128
BssMI GATC 7 cut(s) 229, 241, 415, 562, 790, 828, 1110
BssNAI GTATAC 1 cut(s) 801
BssSI CACGAG 2 cut(s) 145, 708
BssT1I CCWWGG 1 cut(s) 1128
Bst1107I GTATAC 1 cut(s) 801
Bst2BI CACGAG 2 cut(s) 145, 708
Bst2UI CCWGG 3 cut(s) 30, 535, 665
Bst4CI ACNGT 1 cut(s) 730
BstAPI GCANNNNNTGC 1 cut(s) 256
BstDEI CTNAG 3 cut(s) 207, 552, 944
BstDSI CCRYGG 1 cut(s) 461
BstF5I GGATG 4 cut(s) 173, 250, 742, 901
BstHHI GCGC 1 cut(s) 355
BstKTI GATC 7 cut(s) 232, 244, 418, 565, 793, 831, 1113
BstMAI GTCTC 3 cut(s) 493, 705, 1070
BstMBI GATC 7 cut(s) 229, 241, 415, 562, 790, 828, 1110
BstMWI GCNNNNNNNGC 1 cut(s) 256
BstNI CCWGG 3 cut(s) 30, 535, 665
BstSCI CCNGG 3 cut(s) 28, 533, 663
BstV1I GCAGC 2 cut(s) 629, 950
BstV2I GAAGAC 1 cut(s) 320
BstZ17I GTATAC 1 cut(s) 801
Bsu15I ATCGAT 1 cut(s) 244
BsuI GTATCC 2 cut(s) 835, 1135
BsuRI GGCC 1 cut(s) 745
BsuTUI ATCGAT 1 cut(s) 244
BtgI CCRYGG 1 cut(s) 461
BtsCI GGATG 4 cut(s) 173, 250, 742, 901
BtsIMutI CAGTG 2 cut(s) 221, 726
CaiI CAGNNNCTG 2 cut(s) 114, 642
CfoI GCGC 1 cut(s) 355
Cfr10I RCCGGY 1 cut(s) 1100
Cfr13I GGNCC 2 cut(s) 723, 976
ClaI ATCGAT 1 cut(s) 244
CsiI ACCWGGT 1 cut(s) 663
Csp6I GTAC 3 cut(s) 480, 634, 862
CviAII CATG 6 cut(s) 77, 86, 406, 650, 715, 760
CviQI GTAC 3 cut(s) 480, 634, 862
DdeI CTNAG 3 cut(s) 207, 552, 944
DpnI GATC 7 cut(s) 231, 243, 417, 564, 792, 830, 1112
DpnII GATC 7 cut(s) 229, 241, 415, 562, 790, 828, 1110
Eco130I CCWWGG 1 cut(s) 1128
Eco31I GGTCTC 1 cut(s) 1070
Eco47I GGWCC 2 cut(s) 723, 976
Eco57I CTGAAG 2 cut(s) 255, 714
Eco88I CYCGRG 1 cut(s) 622
EcoO109I RGGNCCY 1 cut(s) 976
EcoRII CCWGG 3 cut(s) 28, 533, 663
EcoT14I CCWWGG 1 cut(s) 1128
EcoT22I ATGCAT 1 cut(s) 62
ErhI CCWWGG 1 cut(s) 1128
FaeI CATG 6 cut(s) 80, 89, 409, 653, 718, 763
FatI CATG 6 cut(s) 76, 85, 405, 649, 714, 759
FbaI TGATCA 1 cut(s) 828
FblI GTMKAC 1 cut(s) 800
Fnu4HI GCNGC 2 cut(s) 643, 939
FokI GGATG 4 cut(s) 180, 237, 749, 888
Fsp4HI GCNGC 2 cut(s) 643, 939
FspBI CTAG 7 cut(s) 159, 261, 267, 447, 603, 753, 837
GlaI GCGC 1 cut(s) 354
GluI GCNGC 2 cut(s) 643, 939
GsuI CTGGAG 2 cut(s) 136, 531
HaeIII GGCC 1 cut(s) 745
HapII CCGG 1 cut(s) 1101
HhaI GCGC 1 cut(s) 355
Hin1II CATG 6 cut(s) 80, 89, 409, 653, 718, 763
Hin6I GCGC 1 cut(s) 353
HinP1I GCGC 1 cut(s) 353
HincII GTYRAC 1 cut(s) 195
HindII GTYRAC 1 cut(s) 195
HindIII AAGCTT 2 cut(s) 68, 823
HinfI GANTC 6 cut(s) 5, 211, 466, 540, 992, 1136
HpaII CCGG 1 cut(s) 1101
HphI GGTGA 1 cut(s) 790
Hpy166II GTNNAC 3 cut(s) 195, 726, 801
Hpy188I TCNGA 4 cut(s) 381, 694, 906, 1065
Hpy188III TCNNGA 5 cut(s) 108, 233, 278, 753, 832
Hpy8I GTNNAC 3 cut(s) 195, 726, 801
HpyAV CCTTC 4 cut(s) 366, 845, 891, 931
HpyCH4III ACNGT 1 cut(s) 730
HpyCH4IV ACGT 1 cut(s) 202
HpyCH4V TGCA 5 cut(s) 60, 677, 763, 938, 1092
HpyF10VI GCNNNNNNNGC 1 cut(s) 256
HpyF3I CTNAG 3 cut(s) 207, 552, 944
HpySE526I ACGT 1 cut(s) 202
Hsp92II CATG 6 cut(s) 80, 89, 409, 653, 718, 763
HspAI GCGC 1 cut(s) 353
Ksp22I TGATCA 1 cut(s) 828
Kzo9I GATC 7 cut(s) 229, 241, 415, 562, 790, 828, 1110
LmnI GCTCC 1 cut(s) 177
Lsp1109I GCAGC 2 cut(s) 629, 950
LweI GCATC 3 cut(s) 47, 259, 298
MabI ACCWGGT 1 cut(s) 663
MaeI CTAG 7 cut(s) 159, 261, 267, 447, 603, 753, 837
MaeII ACGT 1 cut(s) 202
MaeIII GTNAC 3 cut(s) 391, 659, 1043
MalI GATC 7 cut(s) 231, 243, 417, 564, 792, 830, 1112
MboI GATC 7 cut(s) 229, 241, 415, 562, 790, 828, 1110
MboII GAAGA 5 cut(s) 320, 884, 1001, 1131, 1145
MfeI CAATTG 1 cut(s) 322
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 5 cut(s) 322, 520, 587, 685, 1066
MlyI GAGTC 1 cut(s) 460
Mph1103I ATGCAT 1 cut(s) 62
MseI TTAA 6 cut(s) 423, 524, 656, 702, 981, 1150
MspI CCGG 1 cut(s) 1101
MspR9I CCNGG 3 cut(s) 30, 535, 665
MunI CAATTG 1 cut(s) 322
Mva1269I GAATGC 1 cut(s) 1092
MvaI CCWGG 3 cut(s) 30, 535, 665
MwoI GCNNNNNNNGC 1 cut(s) 256
NdeII GATC 7 cut(s) 229, 241, 415, 562, 790, 828, 1110
NlaIII CATG 6 cut(s) 80, 89, 409, 653, 718, 763
NmuCI GTSAC 2 cut(s) 391, 659
NsiI ATGCAT 1 cut(s) 62
PcsI WCGNNNNNNNCGW 2 cut(s) 365, 629
PctI GAATGC 1 cut(s) 1092
PfeI GAWTC 5 cut(s) 5, 211, 540, 992, 1136
PflFI GACNNNGTC 1 cut(s) 721
PflMI CCANNNNNTGG 2 cut(s) 114, 1103
PkrI GCNGC 2 cut(s) 644, 940
PleI GAGTC 1 cut(s) 460
PpsI GAGTC 1 cut(s) 460
PpuMI RGGWCCY 1 cut(s) 976
Psp1406I AACGTT 1 cut(s) 202
Psp5II RGGWCCY 1 cut(s) 976
Psp6I CCWGG 3 cut(s) 28, 533, 663
PspGI CCWGG 3 cut(s) 28, 533, 663
PspPI GGNCC 2 cut(s) 723, 976
PspPPI RGGWCCY 1 cut(s) 976
PstNI CAGNNNCTG 2 cut(s) 114, 642
PsyI GACNNNGTC 1 cut(s) 721
RsaI GTAC 3 cut(s) 481, 635, 863
RsaNI GTAC 3 cut(s) 480, 634, 862
SaqAI TTAA 6 cut(s) 423, 524, 656, 702, 981, 1150
SatI GCNGC 2 cut(s) 643, 939
Sau3AI GATC 7 cut(s) 229, 241, 415, 562, 790, 828, 1110
Sau96I GGNCC 2 cut(s) 723, 976
SchI GAGTC 1 cut(s) 460
ScrFI CCNGG 3 cut(s) 30, 535, 665
SduI GDGCHC 1 cut(s) 96
SexAI ACCWGGT 1 cut(s) 663
SfaNI GCATC 3 cut(s) 47, 259, 298
SinI GGWCC 2 cut(s) 723, 976
Sse9I AATT 5 cut(s) 322, 520, 587, 685, 1066
SsiI CCGC 1 cut(s) 409
SspMI CTAG 7 cut(s) 159, 261, 267, 447, 603, 753, 837
StyD4I CCNGG 3 cut(s) 28, 533, 663
StyI CCWWGG 1 cut(s) 1128
TaaI ACNGT 1 cut(s) 730
TaiI ACGT 1 cut(s) 205
TaqI TCGA 4 cut(s) 12, 96, 244, 1109
TasI AATT 5 cut(s) 322, 520, 587, 685, 1066
TatI WGTACW 1 cut(s) 479
TfiI GAWTC 5 cut(s) 5, 211, 540, 992, 1136
Tru1I TTAA 6 cut(s) 423, 524, 656, 702, 981, 1150
Tru9I TTAA 6 cut(s) 423, 524, 656, 702, 981, 1150
TscAI CASTG 2 cut(s) 221, 733
TseFI GTSAC 2 cut(s) 391, 659
TseI GCWGC 2 cut(s) 642, 938
Tsp45I GTSAC 2 cut(s) 391, 659
TspDTI ATGAA 5 cut(s) 638, 678, 797, 864, 966
TspGWI ACGGA 3 cut(s) 212, 408, 478
TspRI CASTG 2 cut(s) 221, 733
Tth111I GACNNNGTC 1 cut(s) 721
Van91I CCANNNNNTGG 2 cut(s) 114, 1103
VpaK11BI GGWCC 2 cut(s) 723, 976
XapI RAATTY 1 cut(s) 685
XbaI TCTAGA 1 cut(s) 752
XmiI GTMKAC 1 cut(s) 800
XspI CTAG 7 cut(s) 159, 261, 267, 447, 603, 753, 837
Zsp2I ATGCAT 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.