Rh4DG281800

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0010405
Physical Location & Seq
Reverse (-)
50402386 .. 50406205
3820 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG281800.1

Sequence Viewer

Length: 1329 bp
ATGTCTATGAATAGGGAATCTCTGCATCAAGCTATCATTCCTGGGTTGCCTGACGACTTGGCATTGAGATGTCTGGCAAAGCTTTCTCATGGATATCATGGACTGGTTGAAAGTGTTTCGAAGACGTGGAGAGATTTGATCCGAAGCTCGGAATATGCCAACTATAAAGCTAGAGAACGATTGTGTGGCAACTGGTTGTTTGTTTTGACTGAATGCTCCAACAATGAGTGGGTTGCTTATGATCCTGAAGCTGATAGGTGGCATCCTTTGCCGAAGATTCCATCTGTCCATGCTGAGGGGAAACAGTATGGGTTTTCATGTATTAGTGTTTGCAGTCGGTTTTTCGTAATTGGAGGTTCCTATTCTCAGCATAATCCTGCATTTCCCCATCAGAGGCCTTTGGTAACAAATGAGGTCATGCAGTTTGATCCGTTTAAGAAACAATGGACCAATGTGGCAAGCATGATTACTCCCCGCTCTCACTTTGCATGTAGCGTTGTATCTGGTAAGATTTATGTTGCTGGGGGACGTAACTTATCTTGCCCCAGAGGGCTTACTCTTGCTGAGGTCTATGATCCAGTGGCTGACAAATGGGAGGAGTTGCCACCAATGCCCCATGCCCAGATGGACTGCTTAGGATTATCCTATAAAGGGAAATTTCATGTTCTTAGCGACCAAGTATGCCTGCAAGACCAAAATACCTCTGAGGTATTTAGCCTATCTGATGGAACATGGTGCACAATGGAAGATGTTTGGCCTTTTTCAAGGGCAATGCAATTTGCAGTTCAGGTGATAGGAGATAATCGAGTGTATACTGTTGTAGATTGGGGTGAGAGCTTTATTAAAACAAGAGATACTGAAAAAGGAGAGTGGTACAACATAGGCTCAGTTCCATCTGTGGTTCTTCCTGATCATTGTCGGCAGCTGGAGGCTTTTGGTTATGGTTTTACTGCCTTACAAAATGAATTATATGTCCTAGGAGGGAAGGCTCTCAAGTGGGAACAATCTGGTGCTGGGAGGTTTGACATTGTCAAGTTGGATGTGGTGAGGGTCTGTAACCCCTTGGTTAAGCCATTAAAATGGAGGGAAACCAGACCCATGATTGGGTCAGCTCGTGGCACTGTCATAGGGTGTGCATCCATGGAAGAAGAATCTTCATGTGACCAAGGCTTTATTCTGTCCTTTCATAGTATAGAACAGGGCCATAACCTTGCTTTGAGGAGAGACACTAGCATTAACTACAAACATTTCAAAAACCATGTCTCACATAGAGGAGATAATGCATACCTGCTAGGAAAGCCGAGCAACCGAAATTTGACAAAGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

442

Amino Acids

49.93

Weight (kDa)

7.22

Isoelectric Point (pI)

37.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 75 - 206 1.1e-08 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 92 - 298 4.3e-18 FKB95, Kelch-repeats domain
Kelch_1 PF01344 158 - 205 1.9e-12 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16250 AT1G16250
fragaria_vesca FvH4_4g21831 FvH4_4g21831 FvH4_4g21831 FvH4_4g21831
malus_domestica MD13G1136500.v1.1 MD16G1131800.v1.1
prunus_persica Prupe.1G209000_v2.0.a1
pyrus_communis pycom13g11890 pycom16g11620
rosa_chinensis RchiOBHm_Chr4g0428071
rosa_laevigata RLG00000007184
rosa_multiflora Rmu_sc0005263.1_g000001
rosa_roxburghii Rroxscaffold_5G00369790
rosa_rugosa Rorug04G0222100
rosa_samantha Rh4AG279300 Rh4BG284700 Rh4CG300400 Rh4DG281800
rosa_wichuraiana Rw4G024270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1296
AccBSI CCGCTC 1 cut(s) 477
AccI GTMKAC 1 cut(s) 812
AciI CCGC 1 cut(s) 475
AclWI GGATC 4 cut(s) 133, 236, 422, 569
AcsI RAATTY 2 cut(s) 656, 1312
AcuI CTGAAG 1 cut(s) 267
AfaI GTAC 1 cut(s) 875
AfiI CCNNNNNNNGG 6 cut(s) 148, 295, 393, 651, 1103, 1104
AgsI TTSAA 3 cut(s) 110, 765, 1252
AjiI CACGTC 1 cut(s) 126
AjnI CCWGG 1 cut(s) 40
AjuI GAANNNNNNNTTGG 2 cut(s) 1158, 1190
AluBI AGCT 8 cut(s) 32, 82, 147, 170, 251, 837, 925, 1112
AluI AGCT 8 cut(s) 32, 82, 147, 170, 251, 837, 925, 1112
Alw21I GWGCWC 1 cut(s) 740
Alw26I GTCTC 2 cut(s) 1218, 1267
Alw44I GTGCAC 1 cut(s) 736
AlwI GGATC 4 cut(s) 133, 236, 422, 569
AlwNI CAGNNNCTG 1 cut(s) 584
AoxI GGCC 3 cut(s) 395, 755, 1201
ApaLI GTGCAC 1 cut(s) 736
ApeKI GCWGC 1 cut(s) 922
ApoI RAATTY 2 cut(s) 656, 1312
ArsI GACNNNNNNTTYG 2 cut(s) 1245, 1277
AspA2I CCTAGG 1 cut(s) 976
AspS9I GGNCC 2 cut(s) 447, 1201
AsuHPI GGTGA 3 cut(s) 802, 842, 1057
AsuII TTCGAA 1 cut(s) 119
AvaII GGWCC 1 cut(s) 447
AvrII CCTAGG 1 cut(s) 976
BaeGI GKGCMC 1 cut(s) 740
BauI CACGAG 1 cut(s) 1113
BbsI GAAGAC 1 cut(s) 128
Bbv12I GWGCWC 1 cut(s) 740
BbvCI CCTCAGC 2 cut(s) 294, 564
BbvI GCAGC 1 cut(s) 934
BccI CCATC 5 cut(s) 289, 396, 619, 719, 901
BciT130I CCWGG 1 cut(s) 42
BclI TGATCA 1 cut(s) 910
BcoDI GTCTC 2 cut(s) 1218, 1267
BfaI CTAG 4 cut(s) 171, 977, 1230, 1292
BfuAI ACCTGC 1 cut(s) 1296
BisI GCNGC 1 cut(s) 923
BlnI CCTAGG 1 cut(s) 976
BlsI GCNGC 1 cut(s) 924
Bme1390I CCNGG 1 cut(s) 42
Bme18I GGWCC 1 cut(s) 447
BmgBI CACGTC 1 cut(s) 126
BmgT120I GGNCC 2 cut(s) 447, 1201
BmiI GGNNCC 1 cut(s) 358
BmrFI CCNGG 1 cut(s) 42
BmsI GCATC 3 cut(s) 34, 271, 1145
BpiI GAAGAC 1 cut(s) 128
BpmI CTGGAG 1 cut(s) 947
Bpu10I CCTNAGC 3 cut(s) 294, 564, 634
Bpu14I TTCGAA 1 cut(s) 119
BpuEI CTTGAG 1 cut(s) 977
BsaJI CCNNGG 5 cut(s) 41, 976, 1062, 1140, 1165
Bsc4I CCNNNNNNNGG 6 cut(s) 148, 295, 393, 651, 1103, 1104
Bse1I ACTGG 3 cut(s) 108, 197, 578
Bse3DI GCAATG 1 cut(s) 777
BseBI CCWGG 1 cut(s) 42
BseDI CCNNGG 5 cut(s) 41, 976, 1062, 1140, 1165
BseGI GGATG 3 cut(s) 262, 1045, 1136
BseLI CCNNNNNNNGG 6 cut(s) 148, 295, 393, 651, 1103, 1104
BseMI GCAATG 1 cut(s) 777
BseMII CTCAG 5 cut(s) 285, 380, 555, 696, 900
BseNI ACTGG 3 cut(s) 108, 197, 578
BseRI GAGGAG 3 cut(s) 611, 1234, 1287
BseSI GKGCMC 1 cut(s) 740
BseXI GCAGC 1 cut(s) 934
BseYI CCCAGC 2 cut(s) 521, 1013
BshFI GGCC 3 cut(s) 397, 757, 1203
BsiHKAI GWGCWC 1 cut(s) 740
BslFI GGGAC 1 cut(s) 540
BslI CCNNNNNNNGG 6 cut(s) 148, 295, 393, 651, 1103, 1104
BsmAI GTCTC 2 cut(s) 1218, 1267
BsmFI GGGAC 1 cut(s) 540
BsmI GAATGC 1 cut(s) 218
BsnI GGCC 3 cut(s) 397, 757, 1203
Bsp119I TTCGAA 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 740
Bsp143I GATC 5 cut(s) 138, 241, 427, 574, 910
Bsp19I CCATGG 1 cut(s) 1140
BspACI CCGC 1 cut(s) 475
BspANI GGCC 3 cut(s) 397, 757, 1203
BspCNI CTCAG 5 cut(s) 286, 379, 556, 697, 899
BspLI GGNNCC 1 cut(s) 358
BspMI ACCTGC 1 cut(s) 1296
BspPI GGATC 4 cut(s) 133, 236, 422, 569
BspT104I TTCGAA 1 cut(s) 119
BsrBI CCGCTC 1 cut(s) 477
BsrDI GCAATG 1 cut(s) 777
BsrI ACTGG 3 cut(s) 108, 197, 578
BssECI CCNNGG 5 cut(s) 41, 976, 1062, 1140, 1165
BssMI GATC 5 cut(s) 138, 241, 427, 574, 910
BssNAI GTATAC 1 cut(s) 813
BssSI CACGAG 1 cut(s) 1113
BssT1I CCWWGG 4 cut(s) 976, 1062, 1140, 1165
Bst1107I GTATAC 1 cut(s) 813
Bst2BI CACGAG 1 cut(s) 1113
Bst2UI CCWGG 1 cut(s) 42
Bst4CI ACNGT 3 cut(s) 306, 817, 1123
BstAPI GCANNNNNTGC 1 cut(s) 268
BstBI TTCGAA 1 cut(s) 119
BstC8I GCNNGC 2 cut(s) 460, 686
BstDEI CTNAG 7 cut(s) 294, 366, 564, 634, 668, 705, 886
BstDSI CCRYGG 1 cut(s) 1140
BstF5I GGATG 3 cut(s) 262, 1045, 1136
BstKTI GATC 5 cut(s) 141, 244, 430, 577, 913
BstMAI GTCTC 2 cut(s) 1218, 1267
BstMBI GATC 5 cut(s) 138, 241, 427, 574, 910
BstMWI GCNNNNNNNGC 3 cut(s) 268, 610, 1297
BstNI CCWGG 1 cut(s) 42
BstNSI RCATGY 1 cut(s) 492
BstSCI CCNGG 1 cut(s) 40
BstSLI GKGCMC 1 cut(s) 740
BstV1I GCAGC 1 cut(s) 934
BstV2I GAAGAC 1 cut(s) 128
BstXI CCANNNNNNTGG 1 cut(s) 1080
BstZ17I GTATAC 1 cut(s) 813
BsuRI GGCC 3 cut(s) 397, 757, 1203
BtgI CCRYGG 1 cut(s) 1140
BtrI CACGTC 1 cut(s) 126
BtsCI GGATG 3 cut(s) 262, 1045, 1136
BtsIMutI CAGTG 2 cut(s) 585, 1119
BveI ACCTGC 1 cut(s) 1296
Cac8I GCNNGC 2 cut(s) 460, 686
CaiI CAGNNNCTG 1 cut(s) 584
Cfr13I GGNCC 2 cut(s) 447, 1201
Csp6I GTAC 1 cut(s) 874
CviQI GTAC 1 cut(s) 874
DdeI CTNAG 7 cut(s) 294, 366, 564, 634, 668, 705, 886
DpnI GATC 5 cut(s) 140, 243, 429, 576, 912
DpnII GATC 5 cut(s) 138, 241, 427, 574, 910
Eco130I CCWWGG 4 cut(s) 976, 1062, 1140, 1165
Eco147I AGGCCT 1 cut(s) 397
Eco32I GATATC 1 cut(s) 95
Eco47I GGWCC 1 cut(s) 447
Eco57I CTGAAG 1 cut(s) 267
EcoRII CCWGG 1 cut(s) 40
EcoRV GATATC 1 cut(s) 95
EcoT14I CCWWGG 4 cut(s) 976, 1062, 1140, 1165
EcoT22I ATGCAT 1 cut(s) 1285
ErhI CCWWGG 4 cut(s) 976, 1062, 1140, 1165
FaqI GGGAC 1 cut(s) 540
FauI CCCGC 1 cut(s) 482
FbaI TGATCA 1 cut(s) 910
FblI GTMKAC 1 cut(s) 812
Fnu4HI GCNGC 1 cut(s) 923
FokI GGATG 3 cut(s) 249, 1052, 1123
Fsp4HI GCNGC 1 cut(s) 923
FspBI CTAG 4 cut(s) 171, 977, 1230, 1292
GluI GCNGC 1 cut(s) 923
GsaI CCCAGC 2 cut(s) 525, 1017
GsuI CTGGAG 1 cut(s) 947
HaeIII GGCC 3 cut(s) 397, 757, 1203
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 3 cut(s) 17, 277, 1151
HphI GGTGA 3 cut(s) 802, 842, 1057
Hpy166II GTNNAC 2 cut(s) 738, 813
Hpy188I TCNGA 5 cut(s) 143, 151, 393, 706, 724
Hpy188III TCNNGA 2 cut(s) 245, 908
Hpy8I GTNNAC 2 cut(s) 738, 813
HpyAV CCTTC 1 cut(s) 979
HpyCH4III ACNGT 3 cut(s) 306, 817, 1123
HpyCH4IV ACGT 2 cut(s) 125, 529
HpyF10VI GCNNNNNNNGC 3 cut(s) 268, 610, 1297
HpyF3I CTNAG 7 cut(s) 294, 366, 564, 634, 668, 705, 886
HpySE526I ACGT 2 cut(s) 125, 529
Ksp22I TGATCA 1 cut(s) 910
Kzo9I GATC 5 cut(s) 138, 241, 427, 574, 910
LmnI GCTCC 1 cut(s) 221
Lsp1109I GCAGC 1 cut(s) 934
LweI GCATC 3 cut(s) 34, 271, 1145
MaeI CTAG 4 cut(s) 171, 977, 1230, 1292
MaeII ACGT 2 cut(s) 125, 529
MaeIII GTNAC 4 cut(s) 403, 530, 1055, 1160
MalI GATC 5 cut(s) 140, 243, 429, 576, 912
MbiI CCGCTC 1 cut(s) 477
MboI GATC 5 cut(s) 138, 241, 427, 574, 910
MboII GAAGA 7 cut(s) 133, 286, 758, 896, 1146, 1157, 1160
MhlI GDGCHC 1 cut(s) 740
MluCI AATT 5 cut(s) 348, 656, 776, 965, 1312
MmeI TCCRAC 2 cut(s) 243, 1017
Mph1103I ATGCAT 1 cut(s) 1285
MseI TTAA 5 cut(s) 435, 843, 1068, 1076, 1236
MslI CAYNNNNRTG 2 cut(s) 67, 1078
MspA1I CMGCKG 1 cut(s) 925
MspR9I CCNGG 1 cut(s) 42
Mva1269I GAATGC 1 cut(s) 218
MvaI CCWGG 1 cut(s) 42
MwoI GCNNNNNNNGC 3 cut(s) 268, 610, 1297
NcoI CCATGG 1 cut(s) 1140
NdeII GATC 5 cut(s) 138, 241, 427, 574, 910
NlaIV GGNNCC 1 cut(s) 358
NmeAIII GCCGAG 1 cut(s) 1326
NmuCI GTSAC 1 cut(s) 1160
NsiI ATGCAT 1 cut(s) 1285
NspI RCATGY 1 cut(s) 492
NspV TTCGAA 1 cut(s) 119
PceI AGGCCT 1 cut(s) 397
PctI GAATGC 1 cut(s) 218
PfeI GAWTC 3 cut(s) 17, 277, 1151
PflFI GACNNNGTC 1 cut(s) 1028
PkrI GCNGC 1 cut(s) 924
Psp6I CCWGG 1 cut(s) 40
PspFI CCCAGC 2 cut(s) 521, 1013
PspGI CCWGG 1 cut(s) 40
PspN4I GGNNCC 1 cut(s) 358
PspPI GGNCC 2 cut(s) 447, 1201
PstNI CAGNNNCTG 1 cut(s) 584
PsyI GACNNNGTC 1 cut(s) 1028
PvuII CAGCTG 1 cut(s) 925
RsaI GTAC 1 cut(s) 875
RsaNI GTAC 1 cut(s) 874
RseI CAYNNNNRTG 2 cut(s) 67, 1078
SaqAI TTAA 5 cut(s) 435, 843, 1068, 1076, 1236
SatI GCNGC 1 cut(s) 923
Sau3AI GATC 5 cut(s) 138, 241, 427, 574, 910
Sau96I GGNCC 2 cut(s) 447, 1201
ScrFI CCNGG 1 cut(s) 42
SduI GDGCHC 1 cut(s) 740
SfaNI GCATC 3 cut(s) 34, 271, 1145
SfuI TTCGAA 1 cut(s) 119
SinI GGWCC 1 cut(s) 447
SmiMI CAYNNNNRTG 2 cut(s) 67, 1078
SmlI CTYRAG 1 cut(s) 992
SmoI CTYRAG 1 cut(s) 992
Sse9I AATT 5 cut(s) 348, 656, 776, 965, 1312
SseBI AGGCCT 1 cut(s) 397
SsiI CCGC 1 cut(s) 475
SspMI CTAG 4 cut(s) 171, 977, 1230, 1292
StuI AGGCCT 1 cut(s) 397
StyD4I CCNGG 1 cut(s) 40
StyI CCWWGG 4 cut(s) 976, 1062, 1140, 1165
TaaI ACNGT 3 cut(s) 306, 817, 1123
TaiI ACGT 2 cut(s) 128, 532
TaqI TCGA 2 cut(s) 119, 805
TasI AATT 5 cut(s) 348, 656, 776, 965, 1312
TfiI GAWTC 3 cut(s) 17, 277, 1151
Tru1I TTAA 5 cut(s) 435, 843, 1068, 1076, 1236
Tru9I TTAA 5 cut(s) 435, 843, 1068, 1076, 1236
TscAI CASTG 2 cut(s) 585, 1126
TseFI GTSAC 1 cut(s) 1160
TseI GCWGC 1 cut(s) 922
Tsp45I GTSAC 1 cut(s) 1160
TspDTI ATGAA 6 cut(s) 23, 306, 650, 978, 1146, 1175
TspGWI ACGGA 1 cut(s) 420
TspRI CASTG 2 cut(s) 585, 1126
Tth111I GACNNNGTC 1 cut(s) 1028
VneI GTGCAC 1 cut(s) 736
VpaK11BI GGWCC 1 cut(s) 447
XapI RAATTY 2 cut(s) 656, 1312
XceI RCATGY 1 cut(s) 492
XmaJI CCTAGG 1 cut(s) 976
XmiI GTMKAC 1 cut(s) 812
XspI CTAG 4 cut(s) 171, 977, 1230, 1292
Zsp2I ATGCAT 1 cut(s) 1285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.