RchiOBHm_Chr4g0428071

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0010405
Physical Location & Seq
Reverse (-)
53159145 .. 53162448
3304 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39698

Sequence Viewer

Length: 1326 bp
ATGATAGTTAACTACTCATGTGTATTGGAATCTCTGCATCAAGCTATCATTCCTGGGTTGCCTGACGACTTGGCATTGAGATGTCTGGCAAAGCTTTCTCATGGATACCGTGGACTGGTTGAAAGTGTTTCGAAGACGTGGAGAGATTTGATCCGAAGCTCCGAATATGCCAACTATAAAGCTAGAGAACGATTGTGTGGCAACTGGTTGTTTGTTTTGACTGAATGCTCCAACAATGAGTGGGTTGCTTATGATCCTGAAGCTGATAGGTGGCATCCTTTGCCGAAGATTCCATCTGTCCATGCTGAGGGGAAACAGTATGGGTTTTCATGTATTAGTGTTTGCAGTCGGTTTTTCGTAATTGGAGGTTCCTATTCTCGGGATAATCCTGCATTTCCCCATCAGAGGCCTTTGGTAACAAATGAGGTCATGCAGTTTGATCCGTTTAAGAAACAATGGACCAATGTGGCAAGCATGATTACTCCCCGCTCTCACTTTGCATGTAGCGTTGTATCTGGTAAGATTTATGTTGCTGGGGGACGTAACTTATCTTGCACCAGAGGGCTTGCTCTTGCTGAGGTCTATGATCCAGTGGCTGACAAATGGGAGGAGTTGCCACCAATGCCCCATGCCCAGATGGACTGCTTAGGATTATCCTATAAAGGGAAATTTCATGTTCTTAGCGACCAAGTGGGCCTGCCAGACCAAAATACCTCTGAGGTTTTTAGCCTATCTGATGGAACATGGTGCACAATGGAAGACGTTTGGCCTTTTCCAAGGGCAATGCAATTTGCAGTTCAGGTGATAGGAGATAATCGAGTGTATACTGTTGTAGATTGGGGTGAGAGCTTTATTAAAACAAGAGATACTGAAAAAGGAGAGTGGTACAACATAGGCTCAGTTCCATCTGTGGTTCTTCCTGATCATTGTCGGCAGCTGGAGGCTTTTGGTTATGGTTTTACTGCCTTACAAAATGAATTATATGTCCTAGGAGGGAAGGCTCTCAAGTGGGAACAATCTGGTGCTGGGAGGTTTGACATTGTCAAGTTGGATGTGGTGAGGGTCTGTAACCCCTTGGTTAAGCCATTAAAATGGAGGGAAACCAGACCCATGATTGGGTCAGCTCGTGGCACTGTCATAGGGTGTGCATCCATGGAAGAAGAATCTTCATGTGACCAAGGCTTTATTCTGTCCTTTCATAGTATAAAACAGGGCCATAACCTTGCTTTGAGGAGAGACACTAGCATCAACTACAAACATTTCAAAAACCATGTCTCACATAGAGGAGATAATGCATACCTGCTAGGTGTTCCCGAAGAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

441

Amino Acids

49.71

Weight (kDa)

6.54

Isoelectric Point (pI)

35.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 79 - 210 9e-09 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 96 - 303 1e-16 FKB95, Kelch-repeats domain
Kelch_1 PF01344 162 - 209 1.1e-12 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16250 AT1G16250
fragaria_vesca FvH4_4g21831 FvH4_4g21831 FvH4_4g21831 FvH4_4g21831
malus_domestica MD13G1136500.v1.1 MD16G1131800.v1.1
prunus_persica Prupe.1G209000_v2.0.a1
pyrus_communis pycom13g11890 pycom16g11620
rosa_chinensis RchiOBHm_Chr4g0428071
rosa_laevigata RLG00000007184
rosa_multiflora Rmu_sc0005263.1_g000001
rosa_roxburghii Rroxscaffold_5G00369790
rosa_rugosa Rorug04G0222100
rosa_samantha Rh4AG279300 Rh4BG284700 Rh4CG300400 Rh4DG281800
rosa_wichuraiana Rw4G024270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1308
AccBSI CCGCTC 1 cut(s) 489
AccI GTMKAC 1 cut(s) 824
AciI CCGC 1 cut(s) 487
AclWI GGATC 4 cut(s) 145, 248, 434, 581
AcsI RAATTY 1 cut(s) 668
AcuI CTGAAG 1 cut(s) 279
AfaI GTAC 1 cut(s) 887
AfiI CCNNNNNNNGG 7 cut(s) 115, 307, 378, 405, 663, 1115, 1116
AgsI TTSAA 2 cut(s) 122, 1264
AjiI CACGTC 1 cut(s) 138
AjnI CCWGG 1 cut(s) 52
AjuI GAANNNNNNNTTGG 2 cut(s) 1170, 1202
AluBI AGCT 8 cut(s) 44, 94, 159, 182, 263, 849, 937, 1124
AluI AGCT 8 cut(s) 44, 94, 159, 182, 263, 849, 937, 1124
Alw21I GWGCWC 1 cut(s) 752
Alw26I GTCTC 2 cut(s) 1230, 1279
Alw44I GTGCAC 1 cut(s) 748
AlwI GGATC 4 cut(s) 145, 248, 434, 581
AlwNI CAGNNNCTG 1 cut(s) 596
Ama87I CYCGRG 1 cut(s) 378
AoxI GGCC 4 cut(s) 407, 694, 767, 1213
ApaLI GTGCAC 1 cut(s) 748
ApeKI GCWGC 1 cut(s) 934
ApoI RAATTY 1 cut(s) 668
ArsI GACNNNNNNTTYG 2 cut(s) 1257, 1289
AspA2I CCTAGG 1 cut(s) 988
AspS9I GGNCC 3 cut(s) 459, 694, 1213
AsuHPI GGTGA 3 cut(s) 814, 854, 1069
AsuII TTCGAA 1 cut(s) 131
AvaI CYCGRG 1 cut(s) 378
AvaII GGWCC 1 cut(s) 459
AvrII CCTAGG 1 cut(s) 988
BaeGI GKGCMC 1 cut(s) 752
BauI CACGAG 1 cut(s) 1125
BbsI GAAGAC 2 cut(s) 140, 765
Bbv12I GWGCWC 1 cut(s) 752
BbvCI CCTCAGC 2 cut(s) 306, 576
BbvI GCAGC 1 cut(s) 946
BccI CCATC 5 cut(s) 301, 408, 631, 731, 913
BciT130I CCWGG 1 cut(s) 54
BciVI GTATCC 1 cut(s) 98
BclI TGATCA 1 cut(s) 922
BcoDI GTCTC 2 cut(s) 1230, 1279
BfaI CTAG 4 cut(s) 183, 989, 1242, 1304
BfuAI ACCTGC 1 cut(s) 1308
BfuI GTATCC 1 cut(s) 98
BisI GCNGC 1 cut(s) 935
BlnI CCTAGG 1 cut(s) 988
BlsI GCNGC 1 cut(s) 936
Bme1390I CCNGG 1 cut(s) 54
Bme18I GGWCC 1 cut(s) 459
BmeT110I CYCGRG 1 cut(s) 378
BmgBI CACGTC 1 cut(s) 138
BmgT120I GGNCC 3 cut(s) 459, 694, 1213
BmiI GGNNCC 1 cut(s) 370
BmrFI CCNGG 1 cut(s) 54
BmsI GCATC 4 cut(s) 46, 283, 1157, 1254
BpiI GAAGAC 2 cut(s) 140, 765
BpmI CTGGAG 1 cut(s) 959
Bpu10I CCTNAGC 3 cut(s) 306, 576, 646
Bpu14I TTCGAA 1 cut(s) 131
BpuEI CTTGAG 1 cut(s) 989
BsaJI CCNNGG 7 cut(s) 53, 109, 776, 988, 1074, 1152, 1177
Bsc4I CCNNNNNNNGG 7 cut(s) 115, 307, 378, 405, 663, 1115, 1116
Bse1I ACTGG 3 cut(s) 120, 209, 590
Bse3DI GCAATG 1 cut(s) 789
BseBI CCWGG 1 cut(s) 54
BseDI CCNNGG 7 cut(s) 53, 109, 776, 988, 1074, 1152, 1177
BseGI GGATG 3 cut(s) 274, 1057, 1148
BseLI CCNNNNNNNGG 7 cut(s) 115, 307, 378, 405, 663, 1115, 1116
BseMI GCAATG 1 cut(s) 789
BseMII CTCAG 4 cut(s) 297, 567, 708, 912
BseNI ACTGG 3 cut(s) 120, 209, 590
BseRI GAGGAG 3 cut(s) 623, 1246, 1299
BseSI GKGCMC 1 cut(s) 752
BseXI GCAGC 1 cut(s) 946
BseYI CCCAGC 2 cut(s) 533, 1025
BshFI GGCC 4 cut(s) 409, 696, 769, 1215
BsiHKAI GWGCWC 1 cut(s) 752
BsiHKCI CYCGRG 1 cut(s) 378
BslFI GGGAC 1 cut(s) 552
BslI CCNNNNNNNGG 7 cut(s) 115, 307, 378, 405, 663, 1115, 1116
BsmAI GTCTC 2 cut(s) 1230, 1279
BsmFI GGGAC 1 cut(s) 552
BsmI GAATGC 1 cut(s) 230
BsnI GGCC 4 cut(s) 409, 696, 769, 1215
BsoBI CYCGRG 1 cut(s) 378
Bsp119I TTCGAA 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 752
Bsp143I GATC 5 cut(s) 150, 253, 439, 586, 922
Bsp19I CCATGG 1 cut(s) 1152
BspACI CCGC 1 cut(s) 487
BspANI GGCC 4 cut(s) 409, 696, 769, 1215
BspCNI CTCAG 4 cut(s) 298, 568, 709, 911
BspLI GGNNCC 1 cut(s) 370
BspMI ACCTGC 1 cut(s) 1308
BspPI GGATC 4 cut(s) 145, 248, 434, 581
BspT104I TTCGAA 1 cut(s) 131
BsrBI CCGCTC 1 cut(s) 489
BsrDI GCAATG 1 cut(s) 789
BsrI ACTGG 3 cut(s) 120, 209, 590
BssECI CCNNGG 7 cut(s) 53, 109, 776, 988, 1074, 1152, 1177
BssMI GATC 5 cut(s) 150, 253, 439, 586, 922
BssNAI GTATAC 1 cut(s) 825
BssSI CACGAG 1 cut(s) 1125
BssT1I CCWWGG 5 cut(s) 776, 988, 1074, 1152, 1177
Bst1107I GTATAC 1 cut(s) 825
Bst2BI CACGAG 1 cut(s) 1125
Bst2UI CCWGG 1 cut(s) 54
Bst4CI ACNGT 4 cut(s) 110, 318, 829, 1135
BstAPI GCANNNNNTGC 1 cut(s) 280
BstBI TTCGAA 1 cut(s) 131
BstC8I GCNNGC 3 cut(s) 472, 567, 698
BstDEI CTNAG 6 cut(s) 306, 576, 646, 680, 717, 898
BstDSI CCRYGG 2 cut(s) 109, 1152
BstF5I GGATG 3 cut(s) 274, 1057, 1148
BstKTI GATC 5 cut(s) 153, 256, 442, 589, 925
BstMAI GTCTC 2 cut(s) 1230, 1279
BstMBI GATC 5 cut(s) 150, 253, 439, 586, 922
BstMWI GCNNNNNNNGC 2 cut(s) 280, 622
BstNI CCWGG 1 cut(s) 54
BstNSI RCATGY 1 cut(s) 504
BstSCI CCNGG 1 cut(s) 52
BstSLI GKGCMC 1 cut(s) 752
BstV1I GCAGC 1 cut(s) 946
BstV2I GAAGAC 2 cut(s) 140, 765
BstXI CCANNNNNNTGG 1 cut(s) 1092
BstZ17I GTATAC 1 cut(s) 825
BsuI GTATCC 1 cut(s) 98
BsuRI GGCC 4 cut(s) 409, 696, 769, 1215
BtgI CCRYGG 2 cut(s) 109, 1152
BtrI CACGTC 1 cut(s) 138
BtsCI GGATG 3 cut(s) 274, 1057, 1148
BtsIMutI CAGTG 2 cut(s) 597, 1131
BveI ACCTGC 1 cut(s) 1308
Cac8I GCNNGC 3 cut(s) 472, 567, 698
CaiI CAGNNNCTG 1 cut(s) 596
Cfr13I GGNCC 3 cut(s) 459, 694, 1213
Csp6I GTAC 1 cut(s) 886
CviQI GTAC 1 cut(s) 886
DdeI CTNAG 6 cut(s) 306, 576, 646, 680, 717, 898
DpnI GATC 5 cut(s) 152, 255, 441, 588, 924
DpnII GATC 5 cut(s) 150, 253, 439, 586, 922
Eco130I CCWWGG 5 cut(s) 776, 988, 1074, 1152, 1177
Eco147I AGGCCT 1 cut(s) 409
Eco47I GGWCC 1 cut(s) 459
Eco57I CTGAAG 1 cut(s) 279
Eco88I CYCGRG 1 cut(s) 378
EcoRII CCWGG 1 cut(s) 52
EcoT14I CCWWGG 5 cut(s) 776, 988, 1074, 1152, 1177
EcoT22I ATGCAT 1 cut(s) 1297
ErhI CCWWGG 5 cut(s) 776, 988, 1074, 1152, 1177
FaqI GGGAC 1 cut(s) 552
FauI CCCGC 1 cut(s) 494
FbaI TGATCA 1 cut(s) 922
FblI GTMKAC 1 cut(s) 824
Fnu4HI GCNGC 1 cut(s) 935
FokI GGATG 3 cut(s) 261, 1064, 1135
Fsp4HI GCNGC 1 cut(s) 935
FspBI CTAG 4 cut(s) 183, 989, 1242, 1304
GluI GCNGC 1 cut(s) 935
GsaI CCCAGC 2 cut(s) 537, 1029
GsuI CTGGAG 1 cut(s) 959
HaeIII GGCC 4 cut(s) 409, 696, 769, 1215
HincII GTYRAC 1 cut(s) 10
HindII GTYRAC 1 cut(s) 10
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 3 cut(s) 29, 289, 1163
HpaI GTTAAC 1 cut(s) 10
HphI GGTGA 3 cut(s) 814, 854, 1069
Hpy166II GTNNAC 4 cut(s) 10, 113, 750, 825
Hpy188I TCNGA 5 cut(s) 155, 163, 405, 718, 736
Hpy188III TCNNGA 4 cut(s) 257, 380, 920, 1313
Hpy8I GTNNAC 4 cut(s) 10, 113, 750, 825
HpyAV CCTTC 1 cut(s) 991
HpyCH4III ACNGT 4 cut(s) 110, 318, 829, 1135
HpyCH4IV ACGT 3 cut(s) 137, 541, 762
HpyF10VI GCNNNNNNNGC 2 cut(s) 280, 622
HpyF3I CTNAG 6 cut(s) 306, 576, 646, 680, 717, 898
HpySE526I ACGT 3 cut(s) 137, 541, 762
Ksp22I TGATCA 1 cut(s) 922
KspAI GTTAAC 1 cut(s) 10
Kzo9I GATC 5 cut(s) 150, 253, 439, 586, 922
LmnI GCTCC 2 cut(s) 164, 233
Lsp1109I GCAGC 1 cut(s) 946
LweI GCATC 4 cut(s) 46, 283, 1157, 1254
MaeI CTAG 4 cut(s) 183, 989, 1242, 1304
MaeII ACGT 3 cut(s) 137, 541, 762
MaeIII GTNAC 4 cut(s) 415, 542, 1067, 1172
MalI GATC 5 cut(s) 152, 255, 441, 588, 924
MbiI CCGCTC 1 cut(s) 489
MboI GATC 5 cut(s) 150, 253, 439, 586, 922
MboII GAAGA 7 cut(s) 145, 298, 770, 908, 1158, 1169, 1172
MhlI GDGCHC 1 cut(s) 752
MluCI AATT 4 cut(s) 360, 668, 788, 977
MmeI TCCRAC 2 cut(s) 255, 1029
Mph1103I ATGCAT 1 cut(s) 1297
MseI TTAA 5 cut(s) 9, 447, 855, 1080, 1088
MslI CAYNNNNRTG 2 cut(s) 79, 1090
MspA1I CMGCKG 1 cut(s) 937
MspR9I CCNGG 1 cut(s) 54
Mva1269I GAATGC 1 cut(s) 230
MvaI CCWGG 1 cut(s) 54
MwoI GCNNNNNNNGC 2 cut(s) 280, 622
NcoI CCATGG 1 cut(s) 1152
NdeII GATC 5 cut(s) 150, 253, 439, 586, 922
NlaIV GGNNCC 1 cut(s) 370
NmuCI GTSAC 1 cut(s) 1172
NsiI ATGCAT 1 cut(s) 1297
NspI RCATGY 1 cut(s) 504
NspV TTCGAA 1 cut(s) 131
PceI AGGCCT 1 cut(s) 409
PctI GAATGC 1 cut(s) 230
PfeI GAWTC 3 cut(s) 29, 289, 1163
PflFI GACNNNGTC 1 cut(s) 1040
PkrI GCNGC 1 cut(s) 936
Psp6I CCWGG 1 cut(s) 52
PspFI CCCAGC 2 cut(s) 533, 1025
PspGI CCWGG 1 cut(s) 52
PspN4I GGNNCC 1 cut(s) 370
PspPI GGNCC 3 cut(s) 459, 694, 1213
PstNI CAGNNNCTG 1 cut(s) 596
PsyI GACNNNGTC 1 cut(s) 1040
PvuII CAGCTG 1 cut(s) 937
RsaI GTAC 1 cut(s) 887
RsaNI GTAC 1 cut(s) 886
RseI CAYNNNNRTG 2 cut(s) 79, 1090
SaqAI TTAA 5 cut(s) 9, 447, 855, 1080, 1088
SatI GCNGC 1 cut(s) 935
Sau3AI GATC 5 cut(s) 150, 253, 439, 586, 922
Sau96I GGNCC 3 cut(s) 459, 694, 1213
ScrFI CCNGG 1 cut(s) 54
SduI GDGCHC 1 cut(s) 752
SfaNI GCATC 4 cut(s) 46, 283, 1157, 1254
SfuI TTCGAA 1 cut(s) 131
SinI GGWCC 1 cut(s) 459
SmiMI CAYNNNNRTG 2 cut(s) 79, 1090
SmlI CTYRAG 1 cut(s) 1004
SmoI CTYRAG 1 cut(s) 1004
Sse9I AATT 4 cut(s) 360, 668, 788, 977
SseBI AGGCCT 1 cut(s) 409
SsiI CCGC 1 cut(s) 487
SspMI CTAG 4 cut(s) 183, 989, 1242, 1304
StuI AGGCCT 1 cut(s) 409
StyD4I CCNGG 1 cut(s) 52
StyI CCWWGG 5 cut(s) 776, 988, 1074, 1152, 1177
TaaI ACNGT 4 cut(s) 110, 318, 829, 1135
TaiI ACGT 3 cut(s) 140, 544, 765
TaqI TCGA 2 cut(s) 131, 817
TasI AATT 4 cut(s) 360, 668, 788, 977
TfiI GAWTC 3 cut(s) 29, 289, 1163
Tru1I TTAA 5 cut(s) 9, 447, 855, 1080, 1088
Tru9I TTAA 5 cut(s) 9, 447, 855, 1080, 1088
TscAI CASTG 2 cut(s) 597, 1138
TseFI GTSAC 1 cut(s) 1172
TseI GCWGC 1 cut(s) 934
Tsp45I GTSAC 1 cut(s) 1172
TspDTI ATGAA 5 cut(s) 318, 662, 990, 1158, 1187
TspGWI ACGGA 1 cut(s) 432
TspRI CASTG 2 cut(s) 597, 1138
Tth111I GACNNNGTC 1 cut(s) 1040
VneI GTGCAC 1 cut(s) 748
VpaK11BI GGWCC 1 cut(s) 459
XapI RAATTY 1 cut(s) 668
XceI RCATGY 1 cut(s) 504
XmaJI CCTAGG 1 cut(s) 988
XmiI GTMKAC 1 cut(s) 824
XspI CTAG 4 cut(s) 183, 989, 1242, 1304
Zsp2I ATGCAT 1 cut(s) 1297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.