Rw4G024270

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0010405
Physical Location & Seq
Reverse (-)
50130533 .. 50133064
2532 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G024270.1

Sequence Viewer

Length: 1332 bp
ATGTGTATTGTTGTTGGGGAATCTCTGCATCAAGCTATCATTCCTGGGTTGCCTGACGACTTGGCATTGAGATGTCTGGCAAAGCTTTCTCATGGATATCATGGACTGGTTGAAAGTGTTTCGAAGACGTGGAGAGATTTGATCCGAAGCTCGGAATATGCCAACTATAAAGCTAGAGAACGATTGTGTGGCAACTGGTTGTTTGTTTTGACTGAATGCTCCAACAATGAGTGGGTTGCTTATGATCCTGAAGCTGATAGGTGGCATCCTTTGCCGAAGATTCCATCTGTCCATGCTGAGGGGAAACAGTATGGGTTTTCATGTATTAGTGTTTGCAGTCGGTTTTTCGTAATTGGAGGTTCCTATTCTCAGCATAATCCTGCATTTCCCCATCAGAGGCCTTTGGTAACAAATGAGGTCATGCAGTTTGATCCGTTTAAGAAACAATGGACCAATGTGGCAAGCATGATTACTCCCCGCTCTCACTTTGCATGTAGCGTTGTATCTGGTAAGATTTATGTTGCTGGGGGACGTAACTTATCTTGCCCCAGAGGGCTTACTCTTGCTGAGGTCTATGATCCAGTGGCTGACAAATGGGAGGAGTTGCCACCAATGCCCCATGCCCAGATGGACTGCTTAGGATTATCCTATAAAGGGAAATTTCATGTTCTTAGCGACCAAGTATGCCTGCAAGACCAAAATACCTCTGAGGTATTTAGCCTATCTGATGGAACATGGTGCACAATGGAAGATGTTTGGCCTTTTTCAAGGGCAATGCAATTTGCAGTTCAGGTGATAGGAGATAATCGAGTGTATACTGTTGTAGATTGGGGTGAGAGCTTTATTAAAACAAGAGATACTGAAAAAGGAGAGTGGTACAACATAGGCTCAGTTCCATCTGTGGTTCTTCCTGATCATTGTCGGCAGCTGGAGGCTTTTGGTTATGGTTTTACTGCCTTACAAAATGAATTATATGTCCTAGGAGGGAAGGCTCTCAAGTGGGAACAATCTGGTGCTGGGAGGTTTGACATTGTCAAGTTGGATGTGGTGAGGGTCTGTAACCCCTTGGTTAAGCCATTAAAATGGAGGGAAACCAGACCCATGATTGGGTCAGCTCGTGGCACTGTCATAGGGTGTGCATCCATGGAAGAAGAATCTTCATGTGACCAAGGCTTTATTCTGTCCTTTCATAGTATAGAACAGGGCCATAACCTTGCTTTGAGGAGAGACACTAGCATTAACTACAAACATTTCAAAAACCATGTCTCACATAGAGGAGATAATGCATACCTGCTAGGAAAGCCGAGCAACCGAAATTTGACAAAGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

443

Amino Acids

49.92

Weight (kDa)

6.95

Isoelectric Point (pI)

36.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 76 - 207 1.1e-08 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 93 - 299 4.3e-18 FKB95, Kelch-repeats domain
Kelch_1 PF01344 159 - 206 1.9e-12 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16250 AT1G16250
fragaria_vesca FvH4_4g21831 FvH4_4g21831 FvH4_4g21831 FvH4_4g21831
malus_domestica MD13G1136500.v1.1 MD16G1131800.v1.1
prunus_persica Prupe.1G209000_v2.0.a1
pyrus_communis pycom13g11890 pycom16g11620
rosa_chinensis RchiOBHm_Chr4g0428071
rosa_laevigata RLG00000007184
rosa_multiflora Rmu_sc0005263.1_g000001
rosa_roxburghii Rroxscaffold_5G00369790
rosa_rugosa Rorug04G0222100
rosa_samantha Rh4AG279300 Rh4BG284700 Rh4CG300400 Rh4DG281800
rosa_wichuraiana Rw4G024270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1299
AccBSI CCGCTC 1 cut(s) 480
AccI GTMKAC 1 cut(s) 815
AciI CCGC 1 cut(s) 478
AclWI GGATC 4 cut(s) 136, 239, 425, 572
AcsI RAATTY 2 cut(s) 659, 1315
AcuI CTGAAG 1 cut(s) 270
AfaI GTAC 1 cut(s) 878
AfiI CCNNNNNNNGG 6 cut(s) 151, 298, 396, 654, 1106, 1107
AgsI TTSAA 3 cut(s) 113, 768, 1255
AjiI CACGTC 1 cut(s) 129
AjnI CCWGG 1 cut(s) 43
AjuI GAANNNNNNNTTGG 2 cut(s) 1161, 1193
AluBI AGCT 8 cut(s) 35, 85, 150, 173, 254, 840, 928, 1115
AluI AGCT 8 cut(s) 35, 85, 150, 173, 254, 840, 928, 1115
Alw21I GWGCWC 1 cut(s) 743
Alw26I GTCTC 2 cut(s) 1221, 1270
Alw44I GTGCAC 1 cut(s) 739
AlwI GGATC 4 cut(s) 136, 239, 425, 572
AlwNI CAGNNNCTG 1 cut(s) 587
AoxI GGCC 3 cut(s) 398, 758, 1204
ApaLI GTGCAC 1 cut(s) 739
ApeKI GCWGC 1 cut(s) 925
ApoI RAATTY 2 cut(s) 659, 1315
ArsI GACNNNNNNTTYG 2 cut(s) 1248, 1280
AspA2I CCTAGG 1 cut(s) 979
AspS9I GGNCC 2 cut(s) 450, 1204
AsuHPI GGTGA 3 cut(s) 805, 845, 1060
AsuII TTCGAA 1 cut(s) 122
AvaII GGWCC 1 cut(s) 450
AvrII CCTAGG 1 cut(s) 979
BaeGI GKGCMC 1 cut(s) 743
BauI CACGAG 1 cut(s) 1116
BbsI GAAGAC 1 cut(s) 131
Bbv12I GWGCWC 1 cut(s) 743
BbvCI CCTCAGC 2 cut(s) 297, 567
BbvI GCAGC 1 cut(s) 937
BccI CCATC 5 cut(s) 292, 399, 622, 722, 904
BciT130I CCWGG 1 cut(s) 45
BclI TGATCA 1 cut(s) 913
BcoDI GTCTC 2 cut(s) 1221, 1270
BfaI CTAG 4 cut(s) 174, 980, 1233, 1295
BfuAI ACCTGC 1 cut(s) 1299
BisI GCNGC 1 cut(s) 926
BlnI CCTAGG 1 cut(s) 979
BlsI GCNGC 1 cut(s) 927
Bme1390I CCNGG 1 cut(s) 45
Bme18I GGWCC 1 cut(s) 450
BmgBI CACGTC 1 cut(s) 129
BmgT120I GGNCC 2 cut(s) 450, 1204
BmiI GGNNCC 1 cut(s) 361
BmrFI CCNGG 1 cut(s) 45
BmsI GCATC 3 cut(s) 37, 274, 1148
BpiI GAAGAC 1 cut(s) 131
BpmI CTGGAG 1 cut(s) 950
Bpu10I CCTNAGC 3 cut(s) 297, 567, 637
Bpu14I TTCGAA 1 cut(s) 122
BpuEI CTTGAG 1 cut(s) 980
BsaJI CCNNGG 5 cut(s) 44, 979, 1065, 1143, 1168
Bsc4I CCNNNNNNNGG 6 cut(s) 151, 298, 396, 654, 1106, 1107
Bse1I ACTGG 3 cut(s) 111, 200, 581
Bse3DI GCAATG 1 cut(s) 780
BseBI CCWGG 1 cut(s) 45
BseDI CCNNGG 5 cut(s) 44, 979, 1065, 1143, 1168
BseGI GGATG 3 cut(s) 265, 1048, 1139
BseLI CCNNNNNNNGG 6 cut(s) 151, 298, 396, 654, 1106, 1107
BseMI GCAATG 1 cut(s) 780
BseMII CTCAG 5 cut(s) 288, 383, 558, 699, 903
BseNI ACTGG 3 cut(s) 111, 200, 581
BseRI GAGGAG 3 cut(s) 614, 1237, 1290
BseSI GKGCMC 1 cut(s) 743
BseXI GCAGC 1 cut(s) 937
BseYI CCCAGC 2 cut(s) 524, 1016
BshFI GGCC 3 cut(s) 400, 760, 1206
BsiHKAI GWGCWC 1 cut(s) 743
BslFI GGGAC 1 cut(s) 543
BslI CCNNNNNNNGG 6 cut(s) 151, 298, 396, 654, 1106, 1107
BsmAI GTCTC 2 cut(s) 1221, 1270
BsmFI GGGAC 1 cut(s) 543
BsmI GAATGC 1 cut(s) 221
BsnI GGCC 3 cut(s) 400, 760, 1206
Bsp119I TTCGAA 1 cut(s) 122
Bsp1286I GDGCHC 1 cut(s) 743
Bsp143I GATC 5 cut(s) 141, 244, 430, 577, 913
Bsp19I CCATGG 1 cut(s) 1143
BspACI CCGC 1 cut(s) 478
BspANI GGCC 3 cut(s) 400, 760, 1206
BspCNI CTCAG 5 cut(s) 289, 382, 559, 700, 902
BspLI GGNNCC 1 cut(s) 361
BspMI ACCTGC 1 cut(s) 1299
BspPI GGATC 4 cut(s) 136, 239, 425, 572
BspT104I TTCGAA 1 cut(s) 122
BsrBI CCGCTC 1 cut(s) 480
BsrDI GCAATG 1 cut(s) 780
BsrI ACTGG 3 cut(s) 111, 200, 581
BssECI CCNNGG 5 cut(s) 44, 979, 1065, 1143, 1168
BssMI GATC 5 cut(s) 141, 244, 430, 577, 913
BssNAI GTATAC 1 cut(s) 816
BssSI CACGAG 1 cut(s) 1116
BssT1I CCWWGG 4 cut(s) 979, 1065, 1143, 1168
Bst1107I GTATAC 1 cut(s) 816
Bst2BI CACGAG 1 cut(s) 1116
Bst2UI CCWGG 1 cut(s) 45
Bst4CI ACNGT 3 cut(s) 309, 820, 1126
BstAPI GCANNNNNTGC 1 cut(s) 271
BstBI TTCGAA 1 cut(s) 122
BstC8I GCNNGC 2 cut(s) 463, 689
BstDEI CTNAG 7 cut(s) 297, 369, 567, 637, 671, 708, 889
BstDSI CCRYGG 1 cut(s) 1143
BstF5I GGATG 3 cut(s) 265, 1048, 1139
BstKTI GATC 5 cut(s) 144, 247, 433, 580, 916
BstMAI GTCTC 2 cut(s) 1221, 1270
BstMBI GATC 5 cut(s) 141, 244, 430, 577, 913
BstMWI GCNNNNNNNGC 3 cut(s) 271, 613, 1300
BstNI CCWGG 1 cut(s) 45
BstNSI RCATGY 1 cut(s) 495
BstSCI CCNGG 1 cut(s) 43
BstSLI GKGCMC 1 cut(s) 743
BstV1I GCAGC 1 cut(s) 937
BstV2I GAAGAC 1 cut(s) 131
BstXI CCANNNNNNTGG 1 cut(s) 1083
BstZ17I GTATAC 1 cut(s) 816
BsuRI GGCC 3 cut(s) 400, 760, 1206
BtgI CCRYGG 1 cut(s) 1143
BtrI CACGTC 1 cut(s) 129
BtsCI GGATG 3 cut(s) 265, 1048, 1139
BtsIMutI CAGTG 2 cut(s) 588, 1122
BveI ACCTGC 1 cut(s) 1299
Cac8I GCNNGC 2 cut(s) 463, 689
CaiI CAGNNNCTG 1 cut(s) 587
Cfr13I GGNCC 2 cut(s) 450, 1204
Csp6I GTAC 1 cut(s) 877
CviQI GTAC 1 cut(s) 877
DdeI CTNAG 7 cut(s) 297, 369, 567, 637, 671, 708, 889
DpnI GATC 5 cut(s) 143, 246, 432, 579, 915
DpnII GATC 5 cut(s) 141, 244, 430, 577, 913
Eco130I CCWWGG 4 cut(s) 979, 1065, 1143, 1168
Eco147I AGGCCT 1 cut(s) 400
Eco32I GATATC 1 cut(s) 98
Eco47I GGWCC 1 cut(s) 450
Eco57I CTGAAG 1 cut(s) 270
EcoRII CCWGG 1 cut(s) 43
EcoRV GATATC 1 cut(s) 98
EcoT14I CCWWGG 4 cut(s) 979, 1065, 1143, 1168
EcoT22I ATGCAT 1 cut(s) 1288
ErhI CCWWGG 4 cut(s) 979, 1065, 1143, 1168
FaqI GGGAC 1 cut(s) 543
FauI CCCGC 1 cut(s) 485
FbaI TGATCA 1 cut(s) 913
FblI GTMKAC 1 cut(s) 815
Fnu4HI GCNGC 1 cut(s) 926
FokI GGATG 3 cut(s) 252, 1055, 1126
Fsp4HI GCNGC 1 cut(s) 926
FspBI CTAG 4 cut(s) 174, 980, 1233, 1295
GluI GCNGC 1 cut(s) 926
GsaI CCCAGC 2 cut(s) 528, 1020
GsuI CTGGAG 1 cut(s) 950
HaeIII GGCC 3 cut(s) 400, 760, 1206
HindIII AAGCTT 1 cut(s) 83
HinfI GANTC 3 cut(s) 20, 280, 1154
HphI GGTGA 3 cut(s) 805, 845, 1060
Hpy166II GTNNAC 2 cut(s) 741, 816
Hpy188I TCNGA 5 cut(s) 146, 154, 396, 709, 727
Hpy188III TCNNGA 2 cut(s) 248, 911
Hpy8I GTNNAC 2 cut(s) 741, 816
HpyAV CCTTC 1 cut(s) 982
HpyCH4III ACNGT 3 cut(s) 309, 820, 1126
HpyCH4IV ACGT 2 cut(s) 128, 532
HpyF10VI GCNNNNNNNGC 3 cut(s) 271, 613, 1300
HpyF3I CTNAG 7 cut(s) 297, 369, 567, 637, 671, 708, 889
HpySE526I ACGT 2 cut(s) 128, 532
Ksp22I TGATCA 1 cut(s) 913
Kzo9I GATC 5 cut(s) 141, 244, 430, 577, 913
LmnI GCTCC 1 cut(s) 224
Lsp1109I GCAGC 1 cut(s) 937
LweI GCATC 3 cut(s) 37, 274, 1148
MaeI CTAG 4 cut(s) 174, 980, 1233, 1295
MaeII ACGT 2 cut(s) 128, 532
MaeIII GTNAC 4 cut(s) 406, 533, 1058, 1163
MalI GATC 5 cut(s) 143, 246, 432, 579, 915
MbiI CCGCTC 1 cut(s) 480
MboI GATC 5 cut(s) 141, 244, 430, 577, 913
MboII GAAGA 7 cut(s) 136, 289, 761, 899, 1149, 1160, 1163
MhlI GDGCHC 1 cut(s) 743
MluCI AATT 5 cut(s) 351, 659, 779, 968, 1315
MmeI TCCRAC 2 cut(s) 246, 1020
Mph1103I ATGCAT 1 cut(s) 1288
MseI TTAA 5 cut(s) 438, 846, 1071, 1079, 1239
MslI CAYNNNNRTG 2 cut(s) 70, 1081
MspA1I CMGCKG 1 cut(s) 928
MspR9I CCNGG 1 cut(s) 45
Mva1269I GAATGC 1 cut(s) 221
MvaI CCWGG 1 cut(s) 45
MwoI GCNNNNNNNGC 3 cut(s) 271, 613, 1300
NcoI CCATGG 1 cut(s) 1143
NdeII GATC 5 cut(s) 141, 244, 430, 577, 913
NlaIV GGNNCC 1 cut(s) 361
NmeAIII GCCGAG 1 cut(s) 1329
NmuCI GTSAC 1 cut(s) 1163
NsiI ATGCAT 1 cut(s) 1288
NspI RCATGY 1 cut(s) 495
NspV TTCGAA 1 cut(s) 122
PceI AGGCCT 1 cut(s) 400
PctI GAATGC 1 cut(s) 221
PfeI GAWTC 3 cut(s) 20, 280, 1154
PflFI GACNNNGTC 1 cut(s) 1031
PkrI GCNGC 1 cut(s) 927
Psp6I CCWGG 1 cut(s) 43
PspFI CCCAGC 2 cut(s) 524, 1016
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 1 cut(s) 361
PspPI GGNCC 2 cut(s) 450, 1204
PstNI CAGNNNCTG 1 cut(s) 587
PsyI GACNNNGTC 1 cut(s) 1031
PvuII CAGCTG 1 cut(s) 928
RsaI GTAC 1 cut(s) 878
RsaNI GTAC 1 cut(s) 877
RseI CAYNNNNRTG 2 cut(s) 70, 1081
SaqAI TTAA 5 cut(s) 438, 846, 1071, 1079, 1239
SatI GCNGC 1 cut(s) 926
Sau3AI GATC 5 cut(s) 141, 244, 430, 577, 913
Sau96I GGNCC 2 cut(s) 450, 1204
ScrFI CCNGG 1 cut(s) 45
SduI GDGCHC 1 cut(s) 743
SfaNI GCATC 3 cut(s) 37, 274, 1148
SfuI TTCGAA 1 cut(s) 122
SinI GGWCC 1 cut(s) 450
SmiMI CAYNNNNRTG 2 cut(s) 70, 1081
SmlI CTYRAG 1 cut(s) 995
SmoI CTYRAG 1 cut(s) 995
Sse9I AATT 5 cut(s) 351, 659, 779, 968, 1315
SseBI AGGCCT 1 cut(s) 400
SsiI CCGC 1 cut(s) 478
SspMI CTAG 4 cut(s) 174, 980, 1233, 1295
StuI AGGCCT 1 cut(s) 400
StyD4I CCNGG 1 cut(s) 43
StyI CCWWGG 4 cut(s) 979, 1065, 1143, 1168
TaaI ACNGT 3 cut(s) 309, 820, 1126
TaiI ACGT 2 cut(s) 131, 535
TaqI TCGA 2 cut(s) 122, 808
TasI AATT 5 cut(s) 351, 659, 779, 968, 1315
TfiI GAWTC 3 cut(s) 20, 280, 1154
Tru1I TTAA 5 cut(s) 438, 846, 1071, 1079, 1239
Tru9I TTAA 5 cut(s) 438, 846, 1071, 1079, 1239
TscAI CASTG 2 cut(s) 588, 1129
TseFI GTSAC 1 cut(s) 1163
TseI GCWGC 1 cut(s) 925
Tsp45I GTSAC 1 cut(s) 1163
TspDTI ATGAA 5 cut(s) 309, 653, 981, 1149, 1178
TspGWI ACGGA 1 cut(s) 423
TspRI CASTG 2 cut(s) 588, 1129
Tth111I GACNNNGTC 1 cut(s) 1031
VneI GTGCAC 1 cut(s) 739
VpaK11BI GGWCC 1 cut(s) 450
XapI RAATTY 2 cut(s) 659, 1315
XceI RCATGY 1 cut(s) 495
XmaJI CCTAGG 1 cut(s) 979
XmiI GTMKAC 1 cut(s) 815
XspI CTAG 4 cut(s) 174, 980, 1233, 1295
Zsp2I ATGCAT 1 cut(s) 1288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.