FvH4_4g21831

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010405
Physical Location & Seq
Reverse (-)
24818170 .. 24822079
3910 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g21831.t2

Sequence Viewer

Length: 1140 bp
ATGGAATCTCTGCATCAAGCTATCATTCCTGGGTTGCCTGATGACTTGGCATTGAGGTGCCTGACAAAGCTGTCTCATGGATACCATGGACTGGTTGAAACTGTTTCAAAGAAATGGAAAGATTTGATCCGAAGCTCGGACTTTGCCAACTATAAAGCGAGAGAGGGATTGTGTGGAAACTGGTTGTTTGTTCTGACTGAAGGCTCTGACAATGAGTGGGTTGCTTATGATCCTGAGGCTGATAGGTGGCATCCTCTGCCTAAGATTCCCTCTGTCCATGCTGAGGGGAGACACTATGGGTTTTCATGTATTAGTGTTTGCAATCAGTTTTTTGTAATTGGAGGGTCCTATTCTCGGGTAAATCCTGCATTTCCTCATCATAGGCCTTTGGTAACAAATGAGGTCATGCAGTTTGATCCATTTCAGAAACAATGGACCAATGTGGCAAGCATGATTACTCCCCGCTCTCACTTTGCATGTAGTGTTGTATCTGGTAAAATTTATGTTGCTGGGGGACGTAACTTATCTTGCACCAGAGGGCTTGCTCTTGCCGAGGTCTATGATCCAGTGGCTGACAAATGGGAGGAGTTGCCACCAATGCCTCATGCCCAGATGGACTGCCTAGGCTTATCCTACAAAGGGAAATTTCATGTTCTTAGCGACCAAGTAGGCCTGCGAGACCAAAATACGTCTGAGGTGTTTAGCTTATCTGATGGAACATGGTGTACAATGGAAGACATTTGGCCTTTTTCAAGGTCAATGCAATTTGCAGTTCAGGTGATAGGAGATAATCGAGTGTATACTGTTGTAGATTGGGGTGAGAGCTTGATTAAAACAAGAGACACTGAAAAAGGAGAGTGGTACAACTTAGGCTCAGTTCCATCTGTGGTTCTTCCTGATCATTGTCGGCAAGTGGAGGCCTTTGGTTATGGTTTTGCTGCCTTACAACATGAATTATATGTCCTAGGAGGAAAGGCTCTCAAGTGGGAACAATCAGGTGCTGGGAGGTTTGACATTGTGAAGTTGGATTTGGTGAGGGTTTGTAACCCCTTGGTTAAGCCATTGAAATGGAGGGAAACTAGACCCATGATTGGGTCAGCTCGTGGCGCTGTCATAGGGTGTGCATCCATGGAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

380

Amino Acids

42.53

Weight (kDa)

6.23

Isoelectric Point (pI)

30.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 70 - 201 4e-09 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 82 - 307 1e-17 FKB95, Kelch-repeats domain
Kelch_1 PF01344 154 - 201 9.2e-13 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16250 AT1G16250
fragaria_vesca FvH4_4g21831 FvH4_4g21831 FvH4_4g21831 FvH4_4g21831
malus_domestica MD13G1136500.v1.1 MD16G1131800.v1.1
prunus_persica Prupe.1G209000_v2.0.a1
pyrus_communis pycom13g11890 pycom16g11620
rosa_chinensis RchiOBHm_Chr4g0428071
rosa_laevigata RLG00000007184
rosa_multiflora Rmu_sc0005263.1_g000001
rosa_roxburghii Rroxscaffold_5G00369790
rosa_rugosa Rorug04G0222100
rosa_samantha Rh4AG279300 Rh4BG284700 Rh4CG300400 Rh4DG281800
rosa_wichuraiana Rw4G024270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 70
AccB1I GGYRCC 1 cut(s) 57
AccB7I CCANNNNNTGG 1 cut(s) 91
AccBSI CCGCTC 1 cut(s) 465
AccI GTMKAC 1 cut(s) 800
AciI CCGC 1 cut(s) 463
AclWI GGATC 4 cut(s) 121, 224, 410, 557
AcsI RAATTY 2 cut(s) 498, 644
AcuI CTGAAG 1 cut(s) 219
AfaI GTAC 2 cut(s) 727, 863
AfiI CCNNNNNNNGG 7 cut(s) 91, 136, 283, 354, 639, 1091, 1092
AgsI TTSAA 4 cut(s) 98, 108, 753, 1066
AjnI CCWGG 1 cut(s) 28
AjuI GAANNNNNNNTTGG 2 cut(s) 1013, 1045
AluBI AGCT 6 cut(s) 20, 70, 135, 705, 825, 1100
AluI AGCT 6 cut(s) 20, 70, 135, 705, 825, 1100
Alw26I GTCTC 4 cut(s) 78, 283, 672, 834
AlwI GGATC 4 cut(s) 121, 224, 410, 557
AlwNI CAGNNNCTG 2 cut(s) 572, 1001
Ama87I CYCGRG 1 cut(s) 354
AoxI GGCC 4 cut(s) 383, 670, 743, 918
ApeKI GCWGC 1 cut(s) 938
ApoI RAATTY 2 cut(s) 498, 644
AspA2I CCTAGG 2 cut(s) 622, 964
AspLEI GCGC 1 cut(s) 1109
AspS9I GGNCC 2 cut(s) 345, 435
AsuHPI GGTGA 3 cut(s) 790, 830, 1045
AvaI CYCGRG 1 cut(s) 354
AvaII GGWCC 2 cut(s) 345, 435
AvrII CCTAGG 2 cut(s) 622, 964
AxyI CCTNAGG 1 cut(s) 234
BanI GGYRCC 1 cut(s) 57
BauI CACGAG 1 cut(s) 1101
BbsI GAAGAC 1 cut(s) 741
BbvCI CCTCAGC 1 cut(s) 282
BbvI GCAGC 1 cut(s) 925
BccI CCATC 3 cut(s) 607, 707, 889
BciT130I CCWGG 1 cut(s) 30
BciVI GTATCC 1 cut(s) 74
BclI TGATCA 1 cut(s) 898
BcoDI GTCTC 4 cut(s) 78, 283, 672, 834
BfaI CTAG 3 cut(s) 623, 965, 1080
BfoI RGCGCY 1 cut(s) 1110
BfuI GTATCC 1 cut(s) 74
BisI GCNGC 1 cut(s) 939
BlnI CCTAGG 2 cut(s) 622, 964
BlsI GCNGC 1 cut(s) 940
Bme1390I CCNGG 1 cut(s) 30
Bme18I GGWCC 2 cut(s) 345, 435
BmeT110I CYCGRG 1 cut(s) 354
BmgT120I GGNCC 2 cut(s) 345, 435
BmiI GGNNCC 2 cut(s) 59, 346
BmrFI CCNGG 1 cut(s) 30
BmsI GCATC 3 cut(s) 22, 259, 1133
BpiI GAAGAC 1 cut(s) 741
Bpu10I CCTNAGC 1 cut(s) 282
BpuEI CTTGAG 1 cut(s) 965
BsaI GGTCTC 1 cut(s) 672
BsaJI CCNNGG 7 cut(s) 29, 85, 552, 622, 964, 1050, 1128
Bsc4I CCNNNNNNNGG 7 cut(s) 91, 136, 283, 354, 639, 1091, 1092
Bse1I ACTGG 3 cut(s) 96, 185, 566
Bse21I CCTNAGG 1 cut(s) 234
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 7 cut(s) 29, 85, 552, 622, 964, 1050, 1128
BseGI GGATG 2 cut(s) 250, 1124
BseLI CCNNNNNNNGG 7 cut(s) 91, 136, 283, 354, 639, 1091, 1092
BseMII CTCAG 4 cut(s) 225, 273, 684, 888
BseNI ACTGG 3 cut(s) 96, 185, 566
BseRI GAGGAG 1 cut(s) 599
BseXI GCAGC 1 cut(s) 925
BseYI CCCAGC 2 cut(s) 509, 1001
BshFI GGCC 4 cut(s) 385, 672, 745, 920
BshNI GGYRCC 1 cut(s) 57
BsiHKCI CYCGRG 1 cut(s) 354
BslFI GGGAC 1 cut(s) 528
BslI CCNNNNNNNGG 7 cut(s) 91, 136, 283, 354, 639, 1091, 1092
BsmAI GTCTC 4 cut(s) 78, 283, 672, 834
BsmFI GGGAC 1 cut(s) 528
BsnI GGCC 4 cut(s) 385, 672, 745, 920
Bso31I GGTCTC 1 cut(s) 672
BsoBI CYCGRG 1 cut(s) 354
Bsp1407I TGTACA 1 cut(s) 725
Bsp143I GATC 5 cut(s) 126, 229, 415, 562, 898
Bsp19I CCATGG 2 cut(s) 85, 1128
BspACI CCGC 1 cut(s) 463
BspANI GGCC 4 cut(s) 385, 672, 745, 920
BspCNI CTCAG 4 cut(s) 226, 274, 685, 887
BspLI GGNNCC 2 cut(s) 59, 346
BspPI GGATC 4 cut(s) 121, 224, 410, 557
BspT107I GGYRCC 1 cut(s) 57
BspTNI GGTCTC 1 cut(s) 672
BsrBI CCGCTC 1 cut(s) 465
BsrGI TGTACA 1 cut(s) 725
BsrI ACTGG 3 cut(s) 96, 185, 566
BssECI CCNNGG 7 cut(s) 29, 85, 552, 622, 964, 1050, 1128
BssMI GATC 5 cut(s) 126, 229, 415, 562, 898
BssNAI GTATAC 1 cut(s) 801
BssSI CACGAG 1 cut(s) 1101
BssT1I CCWWGG 5 cut(s) 85, 622, 964, 1050, 1128
Bst1107I GTATAC 1 cut(s) 801
Bst2BI CACGAG 1 cut(s) 1101
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 2 cut(s) 103, 805
BstAPI GCANNNNNTGC 1 cut(s) 256
BstAUI TGTACA 1 cut(s) 725
BstC8I GCNNGC 3 cut(s) 448, 543, 674
BstDEI CTNAG 7 cut(s) 234, 261, 282, 656, 693, 868, 874
BstDSI CCRYGG 2 cut(s) 85, 1128
BstF5I GGATG 2 cut(s) 250, 1124
BstH2I RGCGCY 1 cut(s) 1110
BstHHI GCGC 1 cut(s) 1109
BstKTI GATC 5 cut(s) 129, 232, 418, 565, 901
BstMAI GTCTC 4 cut(s) 78, 283, 672, 834
BstMBI GATC 5 cut(s) 126, 229, 415, 562, 898
BstMWI GCNNNNNNNGC 3 cut(s) 256, 598, 1106
BstNI CCWGG 1 cut(s) 30
BstNSI RCATGY 1 cut(s) 480
BstSCI CCNGG 1 cut(s) 28
BstV1I GCAGC 1 cut(s) 925
BstV2I GAAGAC 1 cut(s) 741
BstXI CCANNNNNNTGG 1 cut(s) 1068
BstZ17I GTATAC 1 cut(s) 801
Bsu36I CCTNAGG 1 cut(s) 234
BsuI GTATCC 1 cut(s) 74
BsuRI GGCC 4 cut(s) 385, 672, 745, 920
BtgI CCRYGG 2 cut(s) 85, 1128
BtsCI GGATG 2 cut(s) 250, 1124
BtsIMutI CAGTG 2 cut(s) 573, 843
Cac8I GCNNGC 3 cut(s) 448, 543, 674
CaiI CAGNNNCTG 2 cut(s) 572, 1001
CfoI GCGC 1 cut(s) 1109
Cfr13I GGNCC 2 cut(s) 345, 435
Csp6I GTAC 2 cut(s) 726, 862
CviQI GTAC 2 cut(s) 726, 862
DdeI CTNAG 7 cut(s) 234, 261, 282, 656, 693, 868, 874
DpnI GATC 5 cut(s) 128, 231, 417, 564, 900
DpnII GATC 5 cut(s) 126, 229, 415, 562, 898
DrdI GACNNNNNNGTC 1 cut(s) 70
DseDI GACNNNNNNGTC 1 cut(s) 70
Eco130I CCWWGG 5 cut(s) 85, 622, 964, 1050, 1128
Eco147I AGGCCT 3 cut(s) 385, 672, 920
Eco31I GGTCTC 1 cut(s) 672
Eco47I GGWCC 2 cut(s) 345, 435
Eco57I CTGAAG 1 cut(s) 219
Eco81I CCTNAGG 1 cut(s) 234
Eco88I CYCGRG 1 cut(s) 354
EcoO109I RGGNCCY 1 cut(s) 345
EcoRII CCWGG 1 cut(s) 28
EcoT14I CCWWGG 5 cut(s) 85, 622, 964, 1050, 1128
ErhI CCWWGG 5 cut(s) 85, 622, 964, 1050, 1128
FaqI GGGAC 1 cut(s) 528
FauI CCCGC 1 cut(s) 470
FbaI TGATCA 1 cut(s) 898
FblI GTMKAC 1 cut(s) 800
Fnu4HI GCNGC 1 cut(s) 939
FokI GGATG 2 cut(s) 237, 1111
Fsp4HI GCNGC 1 cut(s) 939
FspBI CTAG 3 cut(s) 623, 965, 1080
GlaI GCGC 1 cut(s) 1108
GluI GCNGC 1 cut(s) 939
GsaI CCCAGC 2 cut(s) 513, 1005
HaeII RGCGCY 1 cut(s) 1110
HaeIII GGCC 4 cut(s) 385, 672, 745, 920
HhaI GCGC 1 cut(s) 1109
Hin6I GCGC 1 cut(s) 1107
HinP1I GCGC 1 cut(s) 1107
HinfI GANTC 2 cut(s) 5, 265
HphI GGTGA 3 cut(s) 790, 830, 1045
Hpy166II GTNNAC 2 cut(s) 726, 801
Hpy188I TCNGA 7 cut(s) 131, 139, 195, 208, 426, 694, 712
Hpy188III TCNNGA 2 cut(s) 233, 896
Hpy8I GTNNAC 2 cut(s) 726, 801
HpyAV CCTTC 1 cut(s) 194
HpyCH4III ACNGT 2 cut(s) 103, 805
HpyCH4IV ACGT 2 cut(s) 517, 689
HpyCH4V TGCA 9 cut(s) 13, 321, 368, 409, 476, 531, 763, 770, 1124
HpyF10VI GCNNNNNNNGC 3 cut(s) 256, 598, 1106
HpyF3I CTNAG 7 cut(s) 234, 261, 282, 656, 693, 868, 874
HpySE526I ACGT 2 cut(s) 517, 689
HspAI GCGC 1 cut(s) 1107
Ksp22I TGATCA 1 cut(s) 898
Kzo9I GATC 5 cut(s) 126, 229, 415, 562, 898
Lsp1109I GCAGC 1 cut(s) 925
LweI GCATC 3 cut(s) 22, 259, 1133
MaeI CTAG 3 cut(s) 623, 965, 1080
MaeII ACGT 2 cut(s) 517, 689
MaeIII GTNAC 3 cut(s) 391, 518, 1043
MalI GATC 5 cut(s) 128, 231, 417, 564, 900
MbiI CCGCTC 1 cut(s) 465
MboI GATC 5 cut(s) 126, 229, 415, 562, 898
MboII GAAGA 2 cut(s) 746, 884
MluCI AATT 5 cut(s) 336, 498, 644, 764, 953
MmeI TCCRAC 1 cut(s) 1005
MseI TTAA 2 cut(s) 831, 1056
MslI CAYNNNNRTG 2 cut(s) 55, 1066
MspR9I CCNGG 1 cut(s) 30
MvaI CCWGG 1 cut(s) 30
MwoI GCNNNNNNNGC 3 cut(s) 256, 598, 1106
NcoI CCATGG 2 cut(s) 85, 1128
NdeII GATC 5 cut(s) 126, 229, 415, 562, 898
NlaIV GGNNCC 2 cut(s) 59, 346
NmeAIII GCCGAG 1 cut(s) 577
NspI RCATGY 1 cut(s) 480
PceI AGGCCT 3 cut(s) 385, 672, 920
PfeI GAWTC 2 cut(s) 5, 265
PflMI CCANNNNNTGG 1 cut(s) 91
PkrI GCNGC 1 cut(s) 940
PpuMI RGGWCCY 1 cut(s) 345
Psp5II RGGWCCY 1 cut(s) 345
Psp6I CCWGG 1 cut(s) 28
PspFI CCCAGC 2 cut(s) 509, 1001
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 2 cut(s) 59, 346
PspPI GGNCC 2 cut(s) 345, 435
PspPPI RGGWCCY 1 cut(s) 345
PsrI GAACNNNNNNTAC 2 cut(s) 709, 741
PstNI CAGNNNCTG 2 cut(s) 572, 1001
RsaI GTAC 2 cut(s) 727, 863
RsaNI GTAC 2 cut(s) 726, 862
RseI CAYNNNNRTG 2 cut(s) 55, 1066
SaqAI TTAA 2 cut(s) 831, 1056
SatI GCNGC 1 cut(s) 939
Sau3AI GATC 5 cut(s) 126, 229, 415, 562, 898
Sau96I GGNCC 2 cut(s) 345, 435
ScrFI CCNGG 1 cut(s) 30
SfaNI GCATC 3 cut(s) 22, 259, 1133
SinI GGWCC 2 cut(s) 345, 435
SmiMI CAYNNNNRTG 2 cut(s) 55, 1066
SmlI CTYRAG 1 cut(s) 980
SmoI CTYRAG 1 cut(s) 980
Sse9I AATT 5 cut(s) 336, 498, 644, 764, 953
SseBI AGGCCT 3 cut(s) 385, 672, 920
SsiI CCGC 1 cut(s) 463
SspMI CTAG 3 cut(s) 623, 965, 1080
StuI AGGCCT 3 cut(s) 385, 672, 920
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 5 cut(s) 85, 622, 964, 1050, 1128
TaaI ACNGT 2 cut(s) 103, 805
TaiI ACGT 2 cut(s) 520, 692
TaqI TCGA 1 cut(s) 793
TasI AATT 5 cut(s) 336, 498, 644, 764, 953
TatI WGTACW 1 cut(s) 725
TfiI GAWTC 2 cut(s) 5, 265
Tru1I TTAA 2 cut(s) 831, 1056
Tru9I TTAA 2 cut(s) 831, 1056
TscAI CASTG 2 cut(s) 573, 850
TseI GCWGC 1 cut(s) 938
TspDTI ATGAA 3 cut(s) 294, 638, 966
TspRI CASTG 2 cut(s) 573, 850
Van91I CCANNNNNTGG 1 cut(s) 91
VpaK11BI GGWCC 2 cut(s) 345, 435
XapI RAATTY 2 cut(s) 498, 644
XceI RCATGY 1 cut(s) 480
XmaJI CCTAGG 2 cut(s) 622, 964
XmiI GTMKAC 1 cut(s) 800
XspI CTAG 3 cut(s) 623, 965, 1080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.