Rh4AG279300

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0010405
Physical Location & Seq
Reverse (-)
59524653 .. 59528195
3543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG279300.1

Sequence Viewer

Length: 1200 bp
ATGGAATCTCTGCATCAAGCTATCATTCCCGGGTTGCCTGATGACTTGGCATTGAGATGTCTGGCAAAGCTTTCTCATGGATACCATGGACTGGTTGAAAGTGTTTCGAAGACGTGGAGAGACTTGATCCGAAGCTCGGAATATGCCAACTATAAAGCTAGAGAACGATTGTGTGGCAACTGGTTGTTTGTTTTGACTGAATGCTCCAACAATGAGTGGGTTGCTTATGATCCTGAGGCTGATAGGTGGCATCCTTTGCCAAAGATTCCATCTGTCCATGCTGAGGGGAAACAGTATGGGTTTTCATGTATTAGTGTTTGCAGTCGGTTTTTCGTAATTGGAGGTTCCTATTCTCGGGATAATCCTGCATTTCCCCATCAGAGGCCTTTGGTAACAAATGAGGTCATGCAGTTTGATCCGTTTAAGAAACAATGGACCAATGTGGCAAGCATGATTACTCCCCGCTCTCACTTTGCATGTAGCGTTGTATCTGGTAAGATTTATGTTGCTGGGGGACGTAACTTATCTTGCACCAGAGGGCTTGCTCTTGCTGAGGTCTATGATCCAGTGGCTGACAAATGGGAGGAGTTGCCACCAATGCCCCATGCCCAGATGGACCGCTTAGGATTATCCTATAAAGGAAAATTTCATGTTCTTAGCGACCAAGTAGGCCTGCCAGACCAAAATACCTCTGAGGTATTTAGCCTATCTGATGGAACATGGTGCACAATGGAAGACGTTTGGCCTTTTTCAAGGGCAATGCAATTTGCAGTTCAGGTGATAGGAGATAATCGAGTGTATACCGTTGTAGATTGGGGTGAGAGCTTTATTAAAACAAGAGATACTGAAAAAGGAGAGTGGTACAACATAGGCTCAGTTCCATCTGTGGTTCTTCCTGATCACTGTCGGCAGCTGGAGGCTTTTGGTTATGGTTTTACTGCCTTACAACATGAATTATATGTCCTAGGAGGGAAGGCTCTCAAGTGGGAACAATCTGGTGCTGGGAGGTTTGACATTGTCAAGTTGGATGTGGTGAGGGTCTGTAACCCCTTGGTTAAGCCATTAAAATGGAGGGAAACCAGACCCATGATTGGGTCAGCTCGTGGCACTGTCATAGGGTGTGCATCCATGGAAGAAGAATCTTCATGTGACCAAGGCTTTATTCTGTTGGGGCGCTTCCGAATTCGTTCCCTTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

399

Amino Acids

44.96

Weight (kDa)

6.6

Isoelectric Point (pI)

35.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 71 - 209 2.8e-09 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 88 - 295 3.3e-17 FKB95, Kelch-repeats domain
Kelch_1 PF01344 154 - 201 9.9e-13 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16250 AT1G16250
fragaria_vesca FvH4_4g21831 FvH4_4g21831 FvH4_4g21831 FvH4_4g21831
malus_domestica MD13G1136500.v1.1 MD16G1131800.v1.1
prunus_persica Prupe.1G209000_v2.0.a1
pyrus_communis pycom13g11890 pycom16g11620
rosa_chinensis RchiOBHm_Chr4g0428071
rosa_laevigata RLG00000007184
rosa_multiflora Rmu_sc0005263.1_g000001
rosa_roxburghii Rroxscaffold_5G00369790
rosa_rugosa Rorug04G0222100
rosa_samantha Rh4AG279300 Rh4BG284700 Rh4CG300400 Rh4DG281800
rosa_wichuraiana Rw4G024270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 91
AccBSI CCGCTC 1 cut(s) 465
AccI GTMKAC 1 cut(s) 800
AciI CCGC 2 cut(s) 463, 619
AclWI GGATC 4 cut(s) 121, 224, 410, 557
AcsI RAATTY 2 cut(s) 644, 1182
AfaI GTAC 1 cut(s) 863
AfiI CCNNNNNNNGG 7 cut(s) 91, 136, 283, 354, 381, 1091, 1092
AgsI TTSAA 2 cut(s) 98, 753
AjiI CACGTC 1 cut(s) 114
AjuI GAANNNNNNNTTGG 2 cut(s) 1146, 1178
AluBI AGCT 7 cut(s) 20, 70, 135, 158, 825, 913, 1100
AluI AGCT 7 cut(s) 20, 70, 135, 158, 825, 913, 1100
Alw21I GWGCWC 1 cut(s) 728
Alw26I GTCTC 1 cut(s) 114
Alw44I GTGCAC 1 cut(s) 724
AlwI GGATC 4 cut(s) 121, 224, 410, 557
AlwNI CAGNNNCTG 1 cut(s) 572
Ama87I CYCGRG 2 cut(s) 29, 354
AoxI GGCC 3 cut(s) 383, 670, 743
ApaLI GTGCAC 1 cut(s) 724
ApeKI GCWGC 1 cut(s) 910
ApoI RAATTY 2 cut(s) 644, 1182
Asp700I GAANNNNTTC 1 cut(s) 1186
AspA2I CCTAGG 1 cut(s) 964
AspLEI GCGC 1 cut(s) 1176
AspS9I GGNCC 2 cut(s) 435, 616
AsuC2I CCSGG 2 cut(s) 30, 31
AsuHPI GGTGA 3 cut(s) 790, 830, 1045
AsuII TTCGAA 1 cut(s) 107
AvaI CYCGRG 2 cut(s) 29, 354
AvaII GGWCC 2 cut(s) 435, 616
AvrII CCTAGG 1 cut(s) 964
AxyI CCTNAGG 1 cut(s) 234
BaeGI GKGCMC 1 cut(s) 728
BauI CACGAG 1 cut(s) 1101
BbsI GAAGAC 2 cut(s) 116, 741
Bbv12I GWGCWC 1 cut(s) 728
BbvCI CCTCAGC 2 cut(s) 282, 552
BbvI GCAGC 1 cut(s) 922
BccI CCATC 5 cut(s) 277, 384, 607, 707, 889
BciVI GTATCC 1 cut(s) 74
BclI TGATCA 1 cut(s) 898
BcnI CCSGG 2 cut(s) 30, 31
BcoDI GTCTC 1 cut(s) 114
BfaI CTAG 2 cut(s) 159, 965
BfoI RGCGCY 1 cut(s) 1177
BfuI GTATCC 1 cut(s) 74
BisI GCNGC 1 cut(s) 911
BlnI CCTAGG 1 cut(s) 964
BlsI GCNGC 1 cut(s) 912
Bme1390I CCNGG 2 cut(s) 30, 31
Bme18I GGWCC 2 cut(s) 435, 616
BmeT110I CYCGRG 2 cut(s) 29, 354
BmgBI CACGTC 1 cut(s) 114
BmgT120I GGNCC 2 cut(s) 435, 616
BmiI GGNNCC 1 cut(s) 346
BmrFI CCNGG 2 cut(s) 30, 31
BmsI GCATC 3 cut(s) 22, 259, 1133
BpiI GAAGAC 2 cut(s) 116, 741
BpmI CTGGAG 1 cut(s) 935
Bpu10I CCTNAGC 3 cut(s) 282, 552, 622
Bpu14I TTCGAA 1 cut(s) 107
BpuEI CTTGAG 1 cut(s) 965
BpuMI CCSGG 2 cut(s) 30, 31
BsaJI CCNNGG 6 cut(s) 29, 85, 964, 1050, 1128, 1153
Bsc4I CCNNNNNNNGG 7 cut(s) 91, 136, 283, 354, 381, 1091, 1092
Bse1I ACTGG 3 cut(s) 96, 185, 566
Bse21I CCTNAGG 1 cut(s) 234
Bse3DI GCAATG 1 cut(s) 765
BseDI CCNNGG 6 cut(s) 29, 85, 964, 1050, 1128, 1153
BseGI GGATG 3 cut(s) 250, 1033, 1124
BseLI CCNNNNNNNGG 7 cut(s) 91, 136, 283, 354, 381, 1091, 1092
BseMI GCAATG 1 cut(s) 765
BseMII CTCAG 5 cut(s) 225, 273, 543, 684, 888
BseNI ACTGG 3 cut(s) 96, 185, 566
BseRI GAGGAG 1 cut(s) 599
BseSI GKGCMC 1 cut(s) 728
BseXI GCAGC 1 cut(s) 922
BseYI CCCAGC 2 cut(s) 509, 1001
BshFI GGCC 3 cut(s) 385, 672, 745
BsiHKAI GWGCWC 1 cut(s) 728
BsiHKCI CYCGRG 2 cut(s) 29, 354
BsiSI CCGG 1 cut(s) 30
BslFI GGGAC 1 cut(s) 528
BslI CCNNNNNNNGG 7 cut(s) 91, 136, 283, 354, 381, 1091, 1092
BsmAI GTCTC 1 cut(s) 114
BsmFI GGGAC 1 cut(s) 528
BsmI GAATGC 1 cut(s) 206
BsnI GGCC 3 cut(s) 385, 672, 745
BsoBI CYCGRG 2 cut(s) 29, 354
Bsp119I TTCGAA 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 728
Bsp143I GATC 5 cut(s) 126, 229, 415, 562, 898
Bsp19I CCATGG 2 cut(s) 85, 1128
BspACI CCGC 2 cut(s) 463, 619
BspANI GGCC 3 cut(s) 385, 672, 745
BspCNI CTCAG 5 cut(s) 226, 274, 544, 685, 887
BspLI GGNNCC 1 cut(s) 346
BspPI GGATC 4 cut(s) 121, 224, 410, 557
BspT104I TTCGAA 1 cut(s) 107
BsrBI CCGCTC 1 cut(s) 465
BsrDI GCAATG 1 cut(s) 765
BsrI ACTGG 3 cut(s) 96, 185, 566
BssECI CCNNGG 6 cut(s) 29, 85, 964, 1050, 1128, 1153
BssMI GATC 5 cut(s) 126, 229, 415, 562, 898
BssNAI GTATAC 1 cut(s) 801
BssSI CACGAG 1 cut(s) 1101
BssT1I CCWWGG 5 cut(s) 85, 964, 1050, 1128, 1153
Bst1107I GTATAC 1 cut(s) 801
Bst2BI CACGAG 1 cut(s) 1101
Bst4CI ACNGT 4 cut(s) 294, 805, 905, 1111
BstAPI GCANNNNNTGC 1 cut(s) 256
BstBI TTCGAA 1 cut(s) 107
BstC8I GCNNGC 3 cut(s) 448, 543, 674
BstDEI CTNAG 7 cut(s) 234, 282, 552, 622, 656, 693, 874
BstDSI CCRYGG 2 cut(s) 85, 1128
BstF5I GGATG 3 cut(s) 250, 1033, 1124
BstH2I RGCGCY 1 cut(s) 1177
BstHHI GCGC 1 cut(s) 1176
BstKTI GATC 5 cut(s) 129, 232, 418, 565, 901
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 5 cut(s) 126, 229, 415, 562, 898
BstMWI GCNNNNNNNGC 2 cut(s) 256, 598
BstNSI RCATGY 1 cut(s) 480
BstSCI CCNGG 2 cut(s) 28, 29
BstSLI GKGCMC 1 cut(s) 728
BstV1I GCAGC 1 cut(s) 922
BstV2I GAAGAC 2 cut(s) 116, 741
BstXI CCANNNNNNTGG 1 cut(s) 1068
BstZ17I GTATAC 1 cut(s) 801
Bsu36I CCTNAGG 1 cut(s) 234
BsuI GTATCC 1 cut(s) 74
BsuRI GGCC 3 cut(s) 385, 672, 745
BtgI CCRYGG 2 cut(s) 85, 1128
BtrI CACGTC 1 cut(s) 114
BtsCI GGATG 3 cut(s) 250, 1033, 1124
BtsIMutI CAGTG 3 cut(s) 573, 901, 1107
Cac8I GCNNGC 3 cut(s) 448, 543, 674
CaiI CAGNNNCTG 1 cut(s) 572
CfoI GCGC 1 cut(s) 1176
Cfr13I GGNCC 2 cut(s) 435, 616
Cfr9I CCCGGG 1 cut(s) 29
Csp6I GTAC 1 cut(s) 862
CviQI GTAC 1 cut(s) 862
DdeI CTNAG 7 cut(s) 234, 282, 552, 622, 656, 693, 874
DpnI GATC 5 cut(s) 128, 231, 417, 564, 900
DpnII GATC 5 cut(s) 126, 229, 415, 562, 898
Eco130I CCWWGG 5 cut(s) 85, 964, 1050, 1128, 1153
Eco147I AGGCCT 2 cut(s) 385, 672
Eco47I GGWCC 2 cut(s) 435, 616
Eco81I CCTNAGG 1 cut(s) 234
Eco88I CYCGRG 2 cut(s) 29, 354
EcoRI GAATTC 1 cut(s) 1182
EcoT14I CCWWGG 5 cut(s) 85, 964, 1050, 1128, 1153
ErhI CCWWGG 5 cut(s) 85, 964, 1050, 1128, 1153
FaqI GGGAC 1 cut(s) 528
FauI CCCGC 1 cut(s) 470
FbaI TGATCA 1 cut(s) 898
FblI GTMKAC 1 cut(s) 800
Fnu4HI GCNGC 1 cut(s) 911
FokI GGATG 3 cut(s) 237, 1040, 1111
Fsp4HI GCNGC 1 cut(s) 911
FspBI CTAG 2 cut(s) 159, 965
GlaI GCGC 1 cut(s) 1175
GluI GCNGC 1 cut(s) 911
GsaI CCCAGC 2 cut(s) 513, 1005
GsuI CTGGAG 1 cut(s) 935
HaeII RGCGCY 1 cut(s) 1177
HaeIII GGCC 3 cut(s) 385, 672, 745
HapII CCGG 1 cut(s) 30
HhaI GCGC 1 cut(s) 1176
Hin6I GCGC 1 cut(s) 1174
HinP1I GCGC 1 cut(s) 1174
HindIII AAGCTT 1 cut(s) 68
HinfI GANTC 3 cut(s) 5, 265, 1139
HpaII CCGG 1 cut(s) 30
HphI GGTGA 3 cut(s) 790, 830, 1045
Hpy166II GTNNAC 2 cut(s) 726, 801
Hpy188I TCNGA 6 cut(s) 131, 139, 381, 694, 712, 1181
Hpy188III TCNNGA 3 cut(s) 233, 356, 896
Hpy8I GTNNAC 2 cut(s) 726, 801
HpyAV CCTTC 1 cut(s) 967
HpyCH4III ACNGT 4 cut(s) 294, 805, 905, 1111
HpyCH4IV ACGT 3 cut(s) 113, 517, 738
HpyF10VI GCNNNNNNNGC 2 cut(s) 256, 598
HpyF3I CTNAG 7 cut(s) 234, 282, 552, 622, 656, 693, 874
HpySE526I ACGT 3 cut(s) 113, 517, 738
HspAI GCGC 1 cut(s) 1174
Ksp22I TGATCA 1 cut(s) 898
Kzo9I GATC 5 cut(s) 126, 229, 415, 562, 898
LmnI GCTCC 1 cut(s) 209
Lsp1109I GCAGC 1 cut(s) 922
LweI GCATC 3 cut(s) 22, 259, 1133
MaeI CTAG 2 cut(s) 159, 965
MaeII ACGT 3 cut(s) 113, 517, 738
MaeIII GTNAC 4 cut(s) 391, 518, 1043, 1148
MalI GATC 5 cut(s) 128, 231, 417, 564, 900
MbiI CCGCTC 1 cut(s) 465
MboI GATC 5 cut(s) 126, 229, 415, 562, 898
MboII GAAGA 6 cut(s) 121, 746, 884, 1134, 1145, 1148
MhlI GDGCHC 1 cut(s) 728
MluCI AATT 5 cut(s) 336, 644, 764, 953, 1182
MmeI TCCRAC 2 cut(s) 231, 1005
MroXI GAANNNNTTC 1 cut(s) 1186
MseI TTAA 4 cut(s) 423, 831, 1056, 1064
MslI CAYNNNNRTG 2 cut(s) 55, 1066
MspA1I CMGCKG 1 cut(s) 913
MspI CCGG 1 cut(s) 30
MspR9I CCNGG 2 cut(s) 30, 31
Mva1269I GAATGC 1 cut(s) 206
MwoI GCNNNNNNNGC 2 cut(s) 256, 598
NciI CCSGG 2 cut(s) 30, 31
NcoI CCATGG 2 cut(s) 85, 1128
NdeII GATC 5 cut(s) 126, 229, 415, 562, 898
NlaIV GGNNCC 1 cut(s) 346
NmuCI GTSAC 1 cut(s) 1148
NspI RCATGY 1 cut(s) 480
NspV TTCGAA 1 cut(s) 107
PceI AGGCCT 2 cut(s) 385, 672
PctI GAATGC 1 cut(s) 206
PdmI GAANNNNTTC 1 cut(s) 1186
PfeI GAWTC 3 cut(s) 5, 265, 1139
PflFI GACNNNGTC 1 cut(s) 1016
PflMI CCANNNNNTGG 1 cut(s) 91
PkrI GCNGC 1 cut(s) 912
PspFI CCCAGC 2 cut(s) 509, 1001
PspN4I GGNNCC 1 cut(s) 346
PspPI GGNCC 2 cut(s) 435, 616
PstNI CAGNNNCTG 1 cut(s) 572
PsyI GACNNNGTC 1 cut(s) 1016
PvuII CAGCTG 1 cut(s) 913
RsaI GTAC 1 cut(s) 863
RsaNI GTAC 1 cut(s) 862
RseI CAYNNNNRTG 2 cut(s) 55, 1066
SaqAI TTAA 4 cut(s) 423, 831, 1056, 1064
SatI GCNGC 1 cut(s) 911
Sau3AI GATC 5 cut(s) 126, 229, 415, 562, 898
Sau96I GGNCC 2 cut(s) 435, 616
ScrFI CCNGG 2 cut(s) 30, 31
SduI GDGCHC 1 cut(s) 728
SfaNI GCATC 3 cut(s) 22, 259, 1133
SfuI TTCGAA 1 cut(s) 107
SinI GGWCC 2 cut(s) 435, 616
SmaI CCCGGG 1 cut(s) 31
SmiMI CAYNNNNRTG 2 cut(s) 55, 1066
SmlI CTYRAG 1 cut(s) 980
SmoI CTYRAG 1 cut(s) 980
Sse9I AATT 5 cut(s) 336, 644, 764, 953, 1182
SseBI AGGCCT 2 cut(s) 385, 672
SsiI CCGC 2 cut(s) 463, 619
SspMI CTAG 2 cut(s) 159, 965
StuI AGGCCT 2 cut(s) 385, 672
StyD4I CCNGG 2 cut(s) 28, 29
StyI CCWWGG 5 cut(s) 85, 964, 1050, 1128, 1153
TaaI ACNGT 4 cut(s) 294, 805, 905, 1111
TaiI ACGT 3 cut(s) 116, 520, 741
TaqI TCGA 2 cut(s) 107, 793
TasI AATT 5 cut(s) 336, 644, 764, 953, 1182
TfiI GAWTC 3 cut(s) 5, 265, 1139
Tru1I TTAA 4 cut(s) 423, 831, 1056, 1064
Tru9I TTAA 4 cut(s) 423, 831, 1056, 1064
TscAI CASTG 3 cut(s) 573, 908, 1114
TseFI GTSAC 1 cut(s) 1148
TseI GCWGC 1 cut(s) 910
Tsp45I GTSAC 1 cut(s) 1148
TspDTI ATGAA 5 cut(s) 294, 638, 966, 1134, 1185
TspGWI ACGGA 1 cut(s) 408
TspMI CCCGGG 1 cut(s) 29
TspRI CASTG 3 cut(s) 573, 908, 1114
Tth111I GACNNNGTC 1 cut(s) 1016
Van91I CCANNNNNTGG 1 cut(s) 91
VneI GTGCAC 1 cut(s) 724
VpaK11BI GGWCC 2 cut(s) 435, 616
XapI RAATTY 2 cut(s) 644, 1182
XceI RCATGY 1 cut(s) 480
XmaI CCCGGG 1 cut(s) 29
XmaJI CCTAGG 1 cut(s) 964
XmiI GTMKAC 1 cut(s) 800
XmnI GAANNNNTTC 1 cut(s) 1186
XspI CTAG 2 cut(s) 159, 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.