MD16G1131800.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010405
Physical Location & Seq
Forward (+)
10054286 .. 10056827
2542 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1131800.v1.1.491

Sequence Viewer

Length: 1164 bp
ATGGTGGAGTCTCTGCACCAGTGTATTATTCCTGGATTGTCTGATGATTTGGCGTTGAGATGCTTGGCAAAGGTGTCTCATGGGTACCATGGATTGCTTGAAACTGTCTCAAAGACGTGGAGAGATTTATTACGCAGCTCGGACTATGCCAATTATAAAGCTAGAGAAGGATGGTGCGGCGACTGGCTGTTTGTTCTTACAGAAGGATCTAACAATGAGTGGGTTGCTTATGATCCTGAAGCTGATAGGTGGCATCCTTTGCCAAAGATTCCCACCGCTCATGGTGACTGGAAACACTTTGGATTTTCAAGTATTGGTGTCTGCAACCGATTTTTCGTTATTGGAGGGTCCTATGCGCAGAATGACCCACCGGTTCCCCACCAAAAGTCTTTGGTAACAAATGAGGTGATGCAGTTTGATCCGTTTAAGAAACAATGGACCAGTGTGGCAAGCATGCTTACACCGCGTACTCATTTTGCATGTAGTGTTGTTTCTGGTAAAATTTACGTTGTTGGGGGCCGCAACTTATCTTGCACCAGAGGGCTTGCTCTTGCTGAGGTTTATGATCCTGTGGCTAATAAATGGGAGGAGTTGCCACCAATGCTCAATCCTCAGATGGACTGCTTAGGCTTGTCATTCAAAGGCAGATTTCATGTTCTCAGTGATCAAGTAGGCCTGCCAGACCAAAATACCTCTGAGGTTTTTACTCCATCCGATGGAACATGGCGCACTGTGGAAGACATCTGGCCTTTTTCCAGGGCAATGCAATTTGCAGCTCAGGTGATCGGAGATAATCGAATATATACTGTTGTAGACTGGGGCGAGAGCTTGATTAAAACAAGGGATACTGAAAAGGGAGAGTGGTACACCATAGGCTCAGTTCCGTCAGTGATTTTTCATGATCACCCTCGGCTGCTGGAGGCTTTTGGGTACGGTTTTGCTGCCTTAAGGCACGAACTATATGTTCTGGGAGGGAAGGCTCTCAGGTGGGAACAATCTGGTGCCGGGAGGTTTGACATTGTGAAGTTGGGTTTGGTTAGGGTTTGCAATCCATCGGCTAAGCCATTAAAATGGAGGGAAACTAGACCGATGATTGGGTCAGCTCGTGGCACCGTTATAGGGTGCGCATCCATGGAAGAAGAATCTTCATTTTTAACACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

388

Amino Acids

43.48

Weight (kDa)

6.46

Isoelectric Point (pI)

30.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 71 - 202 1.9e-08 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 85 - 291 4.7e-16 FKB95, Kelch-repeats domain
Beta-prop_FBX42 PF13415 104 - 197 8.7e-06 FBX42, beta-propeller domain
Kelch_1 PF01344 155 - 201 5.8e-13 Kelch motif
Kelch_2 PF07646 155 - 201 8.8e-06 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16250 AT1G16250
fragaria_vesca FvH4_4g21831 FvH4_4g21831 FvH4_4g21831 FvH4_4g21831
malus_domestica MD13G1136500.v1.1 MD16G1131800.v1.1
prunus_persica Prupe.1G209000_v2.0.a1
pyrus_communis pycom13g11890 pycom16g11620
rosa_chinensis RchiOBHm_Chr4g0428071
rosa_laevigata RLG00000007184
rosa_multiflora Rmu_sc0005263.1_g000001
rosa_roxburghii Rroxscaffold_5G00369790
rosa_rugosa Rorug04G0222100
rosa_samantha Rh4AG279300 Rh4BG284700 Rh4CG300400 Rh4DG281800
rosa_wichuraiana Rw4G024270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 156
Acc16I TGCGCA 2 cut(s) 357, 1126
Acc65I GGTACC 1 cut(s) 84
AccB1I GGYRCC 3 cut(s) 84, 1001, 1109
AccB7I CCANNNNNTGG 1 cut(s) 716
AccBSI CCGCTC 1 cut(s) 278
AccI GTMKAC 1 cut(s) 813
AccII CGCG 1 cut(s) 466
AciI CCGC 4 cut(s) 177, 276, 464, 520
AclWI GGATC 4 cut(s) 214, 227, 413, 560
AcsI RAATTY 1 cut(s) 501
AcuI CTGAAG 1 cut(s) 258
AfaI GTAC 4 cut(s) 86, 469, 866, 932
AfiI CCNNNNNNNGG 3 cut(s) 716, 1094, 1119
AflII CTTAAG 1 cut(s) 946
AgeI ACCGGT 1 cut(s) 370
AgsI TTSAA 3 cut(s) 101, 309, 640
AjiI CACGTC 1 cut(s) 117
AjnI CCWGG 2 cut(s) 31, 755
AjuI GAANNNNNNNTTGG 2 cut(s) 1016, 1048
AluBI AGCT 6 cut(s) 138, 161, 242, 776, 828, 1103
AluI AGCT 6 cut(s) 138, 161, 242, 776, 828, 1103
Alw26I GTCTC 3 cut(s) 15, 81, 112
AlwI GGATC 4 cut(s) 214, 227, 413, 560
AoxI GGCC 3 cut(s) 517, 673, 746
ApeKI GCWGC 4 cut(s) 135, 773, 913, 941
ApoI RAATTY 1 cut(s) 501
ArsI GACNNNNNNTTYG 2 cut(s) 173, 205
AsiGI ACCGGT 1 cut(s) 370
Asp718I GGTACC 1 cut(s) 84
AspLEI GCGC 3 cut(s) 358, 729, 1127
AspS9I GGNCC 3 cut(s) 348, 438, 517
AsuC2I CCSGG 1 cut(s) 1006
AsuHPI GGTGA 4 cut(s) 296, 418, 793, 896
AvaII GGWCC 2 cut(s) 348, 438
BanI GGYRCC 3 cut(s) 84, 1001, 1109
BauI CACGAG 1 cut(s) 1104
BbsI GAAGAC 1 cut(s) 744
BbvCI CCTCAGC 1 cut(s) 555
BbvI GCAGC 4 cut(s) 147, 785, 900, 928
BccI CCATC 5 cut(s) 165, 610, 710, 718, 1060
BciT130I CCWGG 2 cut(s) 33, 757
BciVI GTATCC 1 cut(s) 838
BclI TGATCA 2 cut(s) 664, 901
BcnI CCSGG 1 cut(s) 1006
BcoDI GTCTC 3 cut(s) 15, 81, 112
BfaI CTAG 2 cut(s) 162, 1083
BfrI CTTAAG 1 cut(s) 946
BfuI GTATCC 1 cut(s) 838
BisI GCNGC 6 cut(s) 136, 178, 520, 774, 914, 942
BlpI GCTNAGC 1 cut(s) 1059
BlsI GCNGC 6 cut(s) 137, 179, 521, 775, 915, 943
Bme1390I CCNGG 3 cut(s) 33, 757, 1006
Bme18I GGWCC 2 cut(s) 348, 438
BmgBI CACGTC 1 cut(s) 117
BmgT120I GGNCC 3 cut(s) 348, 438, 517
BmiI GGNNCC 6 cut(s) 86, 349, 375, 518, 1003, 1111
BmrFI CCNGG 3 cut(s) 33, 757, 1006
BmrI ACTGGG 1 cut(s) 826
BmsI GCATC 4 cut(s) 50, 262, 399, 1136
BmuI ACTGGG 1 cut(s) 826
BpiI GAAGAC 1 cut(s) 744
BpmI CTGGAG 1 cut(s) 938
Bpu10I CCTNAGC 3 cut(s) 555, 625, 777
Bpu1102I GCTNAGC 1 cut(s) 1059
BpuMI CCSGG 1 cut(s) 1006
BsaJI CCNNGG 4 cut(s) 88, 756, 908, 1131
BsaWI WCCGGW 1 cut(s) 370
Bsc4I CCNNNNNNNGG 3 cut(s) 716, 1094, 1119
Bse118I RCCGGY 1 cut(s) 370
Bse1I ACTGG 5 cut(s) 19, 188, 293, 441, 821
Bse3DI GCAATG 1 cut(s) 768
BseBI CCWGG 2 cut(s) 33, 757
BseDI CCNNGG 4 cut(s) 88, 756, 908, 1131
BseGI GGATG 4 cut(s) 176, 253, 710, 1127
BseLI CCNNNNNNNGG 3 cut(s) 716, 1094, 1119
BseMI GCAATG 1 cut(s) 768
BseMII CTCAG 7 cut(s) 546, 626, 673, 687, 791, 891, 997
BseNI ACTGG 5 cut(s) 19, 188, 293, 441, 821
BseRI GAGGAG 1 cut(s) 602
BseXI GCAGC 4 cut(s) 147, 785, 900, 928
Bsh1236I CGCG 1 cut(s) 466
BshFI GGCC 3 cut(s) 519, 675, 748
BshNI GGYRCC 3 cut(s) 84, 1001, 1109
BshTI ACCGGT 1 cut(s) 370
BsiSI CCGG 2 cut(s) 371, 1005
BslI CCNNNNNNNGG 3 cut(s) 716, 1094, 1119
BsmAI GTCTC 3 cut(s) 15, 81, 112
BsnI GGCC 3 cut(s) 519, 675, 748
Bsp143I GATC 7 cut(s) 206, 232, 418, 565, 664, 783, 901
Bsp1720I GCTNAGC 1 cut(s) 1059
Bsp19I CCATGG 2 cut(s) 88, 1131
BspACI CCGC 4 cut(s) 177, 276, 464, 520
BspANI GGCC 3 cut(s) 519, 675, 748
BspCNI CTCAG 7 cut(s) 547, 625, 672, 688, 790, 890, 996
BspFNI CGCG 1 cut(s) 466
BspHI TCATGA 1 cut(s) 898
BspLI GGNNCC 6 cut(s) 86, 349, 375, 518, 1003, 1111
BspPI GGATC 4 cut(s) 214, 227, 413, 560
BspT107I GGYRCC 3 cut(s) 84, 1001, 1109
BspTI CTTAAG 1 cut(s) 946
BsrBI CCGCTC 1 cut(s) 278
BsrDI GCAATG 1 cut(s) 768
BsrFI RCCGGY 1 cut(s) 370
BsrI ACTGG 5 cut(s) 19, 188, 293, 441, 821
BssAI RCCGGY 1 cut(s) 370
BssECI CCNNGG 4 cut(s) 88, 756, 908, 1131
BssMI GATC 7 cut(s) 206, 232, 418, 565, 664, 783, 901
BssSI CACGAG 1 cut(s) 1104
BssT1I CCWWGG 2 cut(s) 88, 1131
Bst2BI CACGAG 1 cut(s) 1104
Bst2UI CCWGG 2 cut(s) 33, 757
Bst4CI ACNGT 6 cut(s) 106, 733, 808, 935, 1114, 1161
BstAFI CTTAAG 1 cut(s) 946
BstAPI GCANNNNNTGC 1 cut(s) 259
BstC8I GCNNGC 4 cut(s) 451, 455, 546, 677
BstDEI CTNAG 9 cut(s) 555, 612, 625, 659, 696, 777, 877, 983, 1059
BstDSI CCRYGG 2 cut(s) 88, 1131
BstF5I GGATG 4 cut(s) 176, 253, 710, 1127
BstFNI CGCG 1 cut(s) 466
BstHHI GCGC 3 cut(s) 358, 729, 1127
BstKTI GATC 7 cut(s) 209, 235, 421, 568, 667, 786, 904
BstMAI GTCTC 3 cut(s) 15, 81, 112
BstMBI GATC 7 cut(s) 206, 232, 418, 565, 664, 783, 901
BstMWI GCNNNNNNNGC 3 cut(s) 259, 463, 601
BstNI CCWGG 2 cut(s) 33, 757
BstNSI RCATGY 2 cut(s) 457, 483
BstSCI CCNGG 3 cut(s) 31, 755, 1004
BstUI CGCG 1 cut(s) 466
BstV1I GCAGC 4 cut(s) 147, 785, 900, 928
BstV2I GAAGAC 1 cut(s) 744
BstX2I RGATCY 1 cut(s) 206
BstXI CCANNNNNNTGG 1 cut(s) 1071
BstYI RGATCY 1 cut(s) 206
BsuI GTATCC 1 cut(s) 838
BsuRI GGCC 3 cut(s) 519, 675, 748
BtgI CCRYGG 2 cut(s) 88, 1131
BtrI CACGTC 1 cut(s) 117
BtsCI GGATG 4 cut(s) 176, 253, 710, 1127
BtsIMutI CAGTG 5 cut(s) 26, 448, 667, 729, 894
Cac8I GCNNGC 4 cut(s) 451, 455, 546, 677
CciI TCATGA 1 cut(s) 898
CfoI GCGC 3 cut(s) 358, 729, 1127
Cfr10I RCCGGY 1 cut(s) 370
Cfr13I GGNCC 3 cut(s) 348, 438, 517
Csp6I GTAC 4 cut(s) 85, 468, 865, 931
CspAI ACCGGT 1 cut(s) 370
CviAII CATG 9 cut(s) 80, 89, 281, 454, 480, 653, 723, 899, 1132
CviQI GTAC 4 cut(s) 85, 468, 865, 931
DdeI CTNAG 9 cut(s) 555, 612, 625, 659, 696, 777, 877, 983, 1059
DpnI GATC 7 cut(s) 208, 234, 420, 567, 666, 785, 903
DpnII GATC 7 cut(s) 206, 232, 418, 565, 664, 783, 901
Eco130I CCWWGG 2 cut(s) 88, 1131
Eco147I AGGCCT 1 cut(s) 675
Eco47I GGWCC 2 cut(s) 348, 438
Eco57I CTGAAG 1 cut(s) 258
EcoO109I RGGNCCY 1 cut(s) 348
EcoRII CCWGG 2 cut(s) 31, 755
EcoT14I CCWWGG 2 cut(s) 88, 1131
ErhI CCWWGG 2 cut(s) 88, 1131
FaeI CATG 9 cut(s) 83, 92, 284, 457, 483, 656, 726, 902, 1135
FatI CATG 9 cut(s) 79, 88, 280, 453, 479, 652, 722, 898, 1131
FbaI TGATCA 2 cut(s) 664, 901
FblI GTMKAC 1 cut(s) 813
Fnu4HI GCNGC 6 cut(s) 136, 178, 520, 774, 914, 942
FokI GGATG 4 cut(s) 183, 240, 697, 1114
Fsp4HI GCNGC 6 cut(s) 136, 178, 520, 774, 914, 942
FspAI RTGCGCAY 1 cut(s) 1126
FspBI CTAG 2 cut(s) 162, 1083
FspI TGCGCA 2 cut(s) 357, 1126
GlaI GCGC 3 cut(s) 357, 728, 1126
GluI GCNGC 6 cut(s) 136, 178, 520, 774, 914, 942
GsuI CTGGAG 1 cut(s) 938
HaeIII GGCC 3 cut(s) 519, 675, 748
HapII CCGG 2 cut(s) 371, 1005
HhaI GCGC 3 cut(s) 358, 729, 1127
Hin1II CATG 9 cut(s) 83, 92, 284, 457, 483, 656, 726, 902, 1135
Hin6I GCGC 3 cut(s) 356, 727, 1125
HinP1I GCGC 3 cut(s) 356, 727, 1125
HinfI GANTC 3 cut(s) 8, 268, 1142
HpaII CCGG 2 cut(s) 371, 1005
HphI GGTGA 4 cut(s) 296, 418, 793, 896
Hpy166II GTNNAC 2 cut(s) 814, 867
Hpy188I TCNGA 6 cut(s) 43, 142, 615, 697, 715, 788
Hpy188III TCNNGA 2 cut(s) 236, 899
Hpy8I GTNNAC 2 cut(s) 814, 867
HpyAV CCTTC 3 cut(s) 161, 197, 970
HpyCH4III ACNGT 6 cut(s) 106, 733, 808, 935, 1114, 1161
HpyCH4IV ACGT 2 cut(s) 116, 507
HpyCH4V TGCA 8 cut(s) 16, 324, 412, 479, 534, 766, 773, 1047
HpyF10VI GCNNNNNNNGC 3 cut(s) 259, 463, 601
HpyF3I CTNAG 9 cut(s) 555, 612, 625, 659, 696, 777, 877, 983, 1059
HpySE526I ACGT 2 cut(s) 116, 507
Hsp92II CATG 9 cut(s) 83, 92, 284, 457, 483, 656, 726, 902, 1135
HspAI GCGC 3 cut(s) 356, 727, 1125
KpnI GGTACC 1 cut(s) 88
Ksp22I TGATCA 2 cut(s) 664, 901
Kzo9I GATC 7 cut(s) 206, 232, 418, 565, 664, 783, 901
Lsp1109I GCAGC 4 cut(s) 147, 785, 900, 928
LweI GCATC 4 cut(s) 50, 262, 399, 1136
MaeI CTAG 2 cut(s) 162, 1083
MaeII ACGT 2 cut(s) 116, 507
MaeIII GTNAC 2 cut(s) 284, 394
MalI GATC 7 cut(s) 208, 234, 420, 567, 666, 785, 903
MbiI CCGCTC 1 cut(s) 278
MboI GATC 7 cut(s) 206, 232, 418, 565, 664, 783, 901
MboII GAAGA 4 cut(s) 749, 1137, 1148, 1151
MflI RGATCY 1 cut(s) 206
MluCI AATT 3 cut(s) 151, 501, 767
MlyI GAGTC 1 cut(s) 17
MseI TTAA 5 cut(s) 426, 834, 947, 1067, 1154
MslI CAYNNNNRTG 1 cut(s) 1069
MspCI CTTAAG 1 cut(s) 946
MspI CCGG 2 cut(s) 371, 1005
MspR9I CCNGG 3 cut(s) 33, 757, 1006
MvaI CCWGG 2 cut(s) 33, 757
MvnI CGCG 1 cut(s) 466
MwoI GCNNNNNNNGC 3 cut(s) 259, 463, 601
NciI CCSGG 1 cut(s) 1006
NcoI CCATGG 2 cut(s) 88, 1131
NdeII GATC 7 cut(s) 206, 232, 418, 565, 664, 783, 901
NlaIII CATG 9 cut(s) 83, 92, 284, 457, 483, 656, 726, 902, 1135
NlaIV GGNNCC 6 cut(s) 86, 349, 375, 518, 1003, 1111
NmeAIII GCCGAG 1 cut(s) 889
NmuCI GTSAC 1 cut(s) 284
NsbI TGCGCA 2 cut(s) 357, 1126
NspI RCATGY 2 cut(s) 457, 483
PaeI GCATGC 1 cut(s) 457
PagI TCATGA 1 cut(s) 898
PceI AGGCCT 1 cut(s) 675
PfeI GAWTC 2 cut(s) 268, 1142
PflMI CCANNNNNTGG 1 cut(s) 716
PfoI TCCNGGA 1 cut(s) 31
PinAI ACCGGT 1 cut(s) 370
PkrI GCNGC 6 cut(s) 137, 179, 521, 775, 915, 943
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
PpuMI RGGWCCY 1 cut(s) 348
PsiI TTATAA 1 cut(s) 156
Psp5II RGGWCCY 1 cut(s) 348
Psp6I CCWGG 2 cut(s) 31, 755
PspGI CCWGG 2 cut(s) 31, 755
PspN4I GGNNCC 6 cut(s) 86, 349, 375, 518, 1003, 1111
PspPI GGNCC 3 cut(s) 348, 438, 517
PspPPI RGGWCCY 1 cut(s) 348
PsuI RGATCY 1 cut(s) 206
RsaI GTAC 4 cut(s) 86, 469, 866, 932
RsaNI GTAC 4 cut(s) 85, 468, 865, 931
RseI CAYNNNNRTG 1 cut(s) 1069
SaqAI TTAA 5 cut(s) 426, 834, 947, 1067, 1154
SatI GCNGC 6 cut(s) 136, 178, 520, 774, 914, 942
Sau3AI GATC 7 cut(s) 206, 232, 418, 565, 664, 783, 901
Sau96I GGNCC 3 cut(s) 348, 438, 517
SchI GAGTC 1 cut(s) 17
ScrFI CCNGG 3 cut(s) 33, 757, 1006
SfaNI GCATC 4 cut(s) 50, 262, 399, 1136
SinI GGWCC 2 cut(s) 348, 438
SmiMI CAYNNNNRTG 1 cut(s) 1069
SmlI CTYRAG 1 cut(s) 946
SmoI CTYRAG 1 cut(s) 946
SphI GCATGC 1 cut(s) 457
Sse9I AATT 3 cut(s) 151, 501, 767
SseBI AGGCCT 1 cut(s) 675
SsiI CCGC 4 cut(s) 177, 276, 464, 520
SspMI CTAG 2 cut(s) 162, 1083
StuI AGGCCT 1 cut(s) 675
StyD4I CCNGG 3 cut(s) 31, 755, 1004
StyI CCWWGG 2 cut(s) 88, 1131
TaaI ACNGT 6 cut(s) 106, 733, 808, 935, 1114, 1161
TaiI ACGT 2 cut(s) 119, 510
TaqI TCGA 1 cut(s) 796
TaqII GACCGA 1 cut(s) 1102
TasI AATT 3 cut(s) 151, 501, 767
TauI GCSGC 2 cut(s) 180, 522
TfiI GAWTC 2 cut(s) 268, 1142
Tru1I TTAA 5 cut(s) 426, 834, 947, 1067, 1154
Tru9I TTAA 5 cut(s) 426, 834, 947, 1067, 1154
TscAI CASTG 5 cut(s) 26, 448, 667, 736, 894
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 4 cut(s) 135, 773, 913, 941
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 3 cut(s) 641, 887, 1137
TspGWI ACGGA 2 cut(s) 411, 873
TspRI CASTG 5 cut(s) 26, 448, 667, 736, 894
Van91I CCANNNNNTGG 1 cut(s) 716
Vha464I CTTAAG 1 cut(s) 946
VpaK11BI GGWCC 2 cut(s) 348, 438
XapI RAATTY 1 cut(s) 501
XceI RCATGY 2 cut(s) 457, 483
XmiI GTMKAC 1 cut(s) 813
XspI CTAG 2 cut(s) 162, 1083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.