Rroxscaffold_5G00369790

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0010405
Physical Location & Seq
Reverse (-)
51580613 .. 51583827
3215 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00369790.1

Sequence Viewer

Length: 1239 bp
ATGGAGGTCCACATATTAAATTTGAAGGTTCAACTAGAACCACCCTTAAAAGAATTTAGGGAATCTCTGCATCAAGCTATCATTCCTGGGTTGCCTGACGACTTGGCATTGAGATGTCTGGCAAAGCTTTCTCATGGATACCATGGACTGGTTGAAAGTGTTTCGAAGACGTGGAGAGATTTGATCCGAAGCTCGGAATATGCCAACTATAAAGCTAGAGAACGATTGTGTGGCAACTGGTTGTTTGTTTTGACTGAATGCTCCAACAATGAGTGGGTTGCTTATGATCCTGAAGCTGATAGGTGGCATCCTTTGCCTAAGATTCCGTCCGCCCATGCTGAGGGGAAACAGTATGGGTTTTCATGTATTAGTGTTTGCAGTTGGTTTTTCGTAATTGGAGGTTCCTATTCTCGGGCTAATCCTGCATTTCCCCATCAGAGGCCTTTGGTAACAAACGAGGTCATGCAGTTTGATCCGTTTAAGAAACAATGGACCAATGTGGCAAGCATGATTACTCCCCGCTCTCACTTTGCATGTAGCGTTGTATCTGGTAAGATTTATGTTGCTGGGGGACGTAACCTATCTTGCACCAGAGGGCTTGTTCTTGCTGAGGTCTATGATCCAGTGGCTGACAAATGGGAGGAGTTGCCACCAATGCCCCATGCCCAGATGGACTGCTTAGGATTATCCTATAAAGGAAAATTTCATGTTCTTAGCGACCAAGTAGGCCTGCCAGACCAAAATACCTCTGAGGTATTTAGCCTATCTGAAGGAACATGGTGCACAATGGAAGACGTTTGGCCTTTTTCAAGGGCAATGCAATTTGCAGTTCAGGTGATAGGAGATAATCAAGTGTATACCGTTGTAGATTGGGGTGAGAGCTTTATTAAAACAAGAGATACTGAAAAAGGAGAGTGGTACAACATAGGCTCAGTTCCATCTGTGGTTCTTCCTGATCACTGTCGGCAGCTGGAGGCTTTTGGTTATGGTTTTACTGCCTTACAACATGAATTATATGTCCTCGGAGGGAAGGCTCTCAAGTGGGAACAATCTGGTGCTGGGAGGTTTGACATTGTCAAGTTGGATGTGGTGAGGGTCTGTAACCCCTTGGTTAAGCCATTAAAATGGAGGGAAACCAGACCCATGATTGGGTCAGCTCGTGGCACTGTCATAGGGTGTGCCTCTATGGAGGAAGAATCTTCATGTGACCAAGGTATAGTTCTAGGCTTGAACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

412

Amino Acids

46.32

Weight (kDa)

5.97

Isoelectric Point (pI)

34.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 90 - 219 3.4e-08 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 108 - 313 2.8e-16 FKB95, Kelch-repeats domain
Kelch_1 PF01344 173 - 220 4.6e-12 Kelch motif
Kelch_2 PF07646 173 - 220 9.2e-06 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16250 AT1G16250
fragaria_vesca FvH4_4g21831 FvH4_4g21831 FvH4_4g21831 FvH4_4g21831
malus_domestica MD13G1136500.v1.1 MD16G1131800.v1.1
prunus_persica Prupe.1G209000_v2.0.a1
pyrus_communis pycom13g11890 pycom16g11620
rosa_chinensis RchiOBHm_Chr4g0428071
rosa_laevigata RLG00000007184
rosa_multiflora Rmu_sc0005263.1_g000001
rosa_roxburghii Rroxscaffold_5G00369790
rosa_rugosa Rorug04G0222100
rosa_samantha Rh4AG279300 Rh4BG284700 Rh4CG300400 Rh4DG281800
rosa_wichuraiana Rw4G024270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 148
AccBSI CCGCTC 1 cut(s) 522
AccI GTMKAC 1 cut(s) 857
AciI CCGC 2 cut(s) 330, 520
AclWI GGATC 4 cut(s) 178, 281, 467, 614
AcsI RAATTY 3 cut(s) 19, 53, 701
AcuI CTGAAG 2 cut(s) 312, 789
AfaI GTAC 1 cut(s) 920
AfiI CCNNNNNNNGG 7 cut(s) 148, 193, 340, 411, 438, 1148, 1149
AgsI TTSAA 5 cut(s) 25, 32, 155, 810, 1231
AjiI CACGTC 1 cut(s) 171
AjnI CCWGG 1 cut(s) 85
AjuI GAANNNNNNNTTGG 2 cut(s) 1203, 1235
AluBI AGCT 8 cut(s) 77, 127, 192, 215, 296, 882, 970, 1157
AluI AGCT 8 cut(s) 77, 127, 192, 215, 296, 882, 970, 1157
Alw21I GWGCWC 1 cut(s) 785
Alw44I GTGCAC 1 cut(s) 781
AlwI GGATC 4 cut(s) 178, 281, 467, 614
AlwNI CAGNNNCTG 1 cut(s) 629
Ama87I CYCGRG 1 cut(s) 411
AoxI GGCC 3 cut(s) 440, 727, 800
ApaLI GTGCAC 1 cut(s) 781
ApeKI GCWGC 1 cut(s) 967
ApoI RAATTY 3 cut(s) 19, 53, 701
AspS9I GGNCC 2 cut(s) 7, 492
AsuHPI GGTGA 3 cut(s) 847, 887, 1102
AsuII TTCGAA 1 cut(s) 164
AvaI CYCGRG 1 cut(s) 411
AvaII GGWCC 2 cut(s) 7, 492
BaeGI GKGCMC 1 cut(s) 785
BauI CACGAG 1 cut(s) 1158
BbsI GAAGAC 2 cut(s) 173, 798
Bbv12I GWGCWC 1 cut(s) 785
BbvCI CCTCAGC 2 cut(s) 339, 609
BbvI GCAGC 1 cut(s) 979
BccI CCATC 3 cut(s) 441, 664, 946
BciT130I CCWGG 1 cut(s) 87
BciVI GTATCC 1 cut(s) 131
BclI TGATCA 1 cut(s) 955
BfaI CTAG 3 cut(s) 35, 216, 1223
BfuI GTATCC 1 cut(s) 131
BisI GCNGC 1 cut(s) 968
BlsI GCNGC 1 cut(s) 969
Bme1390I CCNGG 1 cut(s) 87
Bme18I GGWCC 2 cut(s) 7, 492
BmeT110I CYCGRG 1 cut(s) 411
BmgBI CACGTC 1 cut(s) 171
BmgT120I GGNCC 2 cut(s) 7, 492
BmiI GGNNCC 1 cut(s) 403
BmrFI CCNGG 1 cut(s) 87
BmsI GCATC 2 cut(s) 79, 316
BpiI GAAGAC 2 cut(s) 173, 798
BpmI CTGGAG 1 cut(s) 992
Bpu10I CCTNAGC 3 cut(s) 339, 609, 679
Bpu14I TTCGAA 1 cut(s) 164
BpuEI CTTGAG 1 cut(s) 1022
BsaJI CCNNGG 5 cut(s) 86, 142, 1021, 1107, 1210
Bsc4I CCNNNNNNNGG 7 cut(s) 148, 193, 340, 411, 438, 1148, 1149
Bse1I ACTGG 3 cut(s) 153, 242, 623
Bse3DI GCAATG 1 cut(s) 822
BseBI CCWGG 1 cut(s) 87
BseDI CCNNGG 5 cut(s) 86, 142, 1021, 1107, 1210
BseGI GGATG 2 cut(s) 307, 1090
BseLI CCNNNNNNNGG 7 cut(s) 148, 193, 340, 411, 438, 1148, 1149
BseMI GCAATG 1 cut(s) 822
BseMII CTCAG 4 cut(s) 330, 600, 741, 945
BseNI ACTGG 3 cut(s) 153, 242, 623
BseRI GAGGAG 1 cut(s) 656
BseSI GKGCMC 1 cut(s) 785
BseXI GCAGC 1 cut(s) 979
BseYI CCCAGC 2 cut(s) 566, 1058
BshFI GGCC 3 cut(s) 442, 729, 802
BsiHKAI GWGCWC 1 cut(s) 785
BsiHKCI CYCGRG 1 cut(s) 411
BslFI GGGAC 1 cut(s) 585
BslI CCNNNNNNNGG 7 cut(s) 148, 193, 340, 411, 438, 1148, 1149
BsmFI GGGAC 1 cut(s) 585
BsmI GAATGC 1 cut(s) 263
BsnI GGCC 3 cut(s) 442, 729, 802
BsoBI CYCGRG 1 cut(s) 411
Bsp119I TTCGAA 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 785
Bsp143I GATC 5 cut(s) 183, 286, 472, 619, 955
Bsp19I CCATGG 1 cut(s) 142
BspACI CCGC 2 cut(s) 330, 520
BspANI GGCC 3 cut(s) 442, 729, 802
BspCNI CTCAG 4 cut(s) 331, 601, 742, 944
BspLI GGNNCC 1 cut(s) 403
BspPI GGATC 4 cut(s) 178, 281, 467, 614
BspT104I TTCGAA 1 cut(s) 164
BsrBI CCGCTC 1 cut(s) 522
BsrDI GCAATG 1 cut(s) 822
BsrI ACTGG 3 cut(s) 153, 242, 623
BssECI CCNNGG 5 cut(s) 86, 142, 1021, 1107, 1210
BssMI GATC 5 cut(s) 183, 286, 472, 619, 955
BssNAI GTATAC 1 cut(s) 858
BssSI CACGAG 1 cut(s) 1158
BssT1I CCWWGG 3 cut(s) 142, 1107, 1210
Bst1107I GTATAC 1 cut(s) 858
Bst2BI CACGAG 1 cut(s) 1158
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 4 cut(s) 351, 862, 962, 1168
BstAPI GCANNNNNTGC 1 cut(s) 313
BstBI TTCGAA 1 cut(s) 164
BstC8I GCNNGC 2 cut(s) 505, 731
BstDEI CTNAG 7 cut(s) 318, 339, 609, 679, 713, 750, 931
BstDSI CCRYGG 1 cut(s) 142
BstF5I GGATG 2 cut(s) 307, 1090
BstKTI GATC 5 cut(s) 186, 289, 475, 622, 958
BstMBI GATC 5 cut(s) 183, 286, 472, 619, 955
BstMWI GCNNNNNNNGC 3 cut(s) 313, 422, 655
BstNI CCWGG 1 cut(s) 87
BstNSI RCATGY 1 cut(s) 537
BstSCI CCNGG 1 cut(s) 85
BstSLI GKGCMC 1 cut(s) 785
BstV1I GCAGC 1 cut(s) 979
BstV2I GAAGAC 2 cut(s) 173, 798
BstXI CCANNNNNNTGG 1 cut(s) 1125
BstZ17I GTATAC 1 cut(s) 858
BsuI GTATCC 1 cut(s) 131
BsuRI GGCC 3 cut(s) 442, 729, 802
BtgI CCRYGG 1 cut(s) 142
BtrI CACGTC 1 cut(s) 171
BtsCI GGATG 2 cut(s) 307, 1090
BtsIMutI CAGTG 3 cut(s) 630, 958, 1164
Cac8I GCNNGC 2 cut(s) 505, 731
CaiI CAGNNNCTG 1 cut(s) 629
Cfr13I GGNCC 2 cut(s) 7, 492
Csp6I GTAC 1 cut(s) 919
CviQI GTAC 1 cut(s) 919
DdeI CTNAG 7 cut(s) 318, 339, 609, 679, 713, 750, 931
DpnI GATC 5 cut(s) 185, 288, 474, 621, 957
DpnII GATC 5 cut(s) 183, 286, 472, 619, 955
EciI GGCGGA 1 cut(s) 319
Eco130I CCWWGG 3 cut(s) 142, 1107, 1210
Eco147I AGGCCT 2 cut(s) 442, 729
Eco47I GGWCC 2 cut(s) 7, 492
Eco57I CTGAAG 2 cut(s) 312, 789
Eco88I CYCGRG 1 cut(s) 411
EcoRII CCWGG 1 cut(s) 85
EcoT14I CCWWGG 3 cut(s) 142, 1107, 1210
ErhI CCWWGG 3 cut(s) 142, 1107, 1210
FaqI GGGAC 1 cut(s) 585
FauI CCCGC 1 cut(s) 527
FbaI TGATCA 1 cut(s) 955
FblI GTMKAC 1 cut(s) 857
Fnu4HI GCNGC 1 cut(s) 968
FokI GGATG 2 cut(s) 294, 1097
Fsp4HI GCNGC 1 cut(s) 968
FspBI CTAG 3 cut(s) 35, 216, 1223
GluI GCNGC 1 cut(s) 968
GsaI CCCAGC 2 cut(s) 570, 1062
GsuI CTGGAG 1 cut(s) 992
HaeIII GGCC 3 cut(s) 442, 729, 802
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 3 cut(s) 62, 322, 1196
HphI GGTGA 3 cut(s) 847, 887, 1102
Hpy166II GTNNAC 3 cut(s) 10, 783, 858
Hpy188I TCNGA 6 cut(s) 188, 196, 438, 751, 769, 1025
Hpy188III TCNNGA 2 cut(s) 290, 953
Hpy8I GTNNAC 3 cut(s) 10, 783, 858
HpyAV CCTTC 3 cut(s) 19, 764, 1024
HpyCH4III ACNGT 4 cut(s) 351, 862, 962, 1168
HpyCH4IV ACGT 3 cut(s) 170, 574, 795
HpyCH4V TGCA 9 cut(s) 70, 378, 425, 466, 533, 588, 783, 820, 827
HpyF10VI GCNNNNNNNGC 3 cut(s) 313, 422, 655
HpyF3I CTNAG 7 cut(s) 318, 339, 609, 679, 713, 750, 931
HpySE526I ACGT 3 cut(s) 170, 574, 795
Ksp22I TGATCA 1 cut(s) 955
Kzo9I GATC 5 cut(s) 183, 286, 472, 619, 955
LmnI GCTCC 1 cut(s) 266
Lsp1109I GCAGC 1 cut(s) 979
LweI GCATC 2 cut(s) 79, 316
MaeI CTAG 3 cut(s) 35, 216, 1223
MaeII ACGT 3 cut(s) 170, 574, 795
MaeIII GTNAC 4 cut(s) 448, 575, 1100, 1205
MalI GATC 5 cut(s) 185, 288, 474, 621, 957
MbiI CCGCTC 1 cut(s) 522
MboI GATC 5 cut(s) 183, 286, 472, 619, 955
MboII GAAGA 5 cut(s) 178, 803, 941, 1191, 1205
MfeI CAATTG 1 cut(s) 1234
MhlI GDGCHC 1 cut(s) 785
MluCI AATT 7 cut(s) 19, 53, 393, 701, 821, 1010, 1234
MmeI TCCRAC 2 cut(s) 288, 1062
MseI TTAA 6 cut(s) 17, 47, 480, 888, 1113, 1121
MslI CAYNNNNRTG 2 cut(s) 112, 1123
MspA1I CMGCKG 1 cut(s) 970
MspR9I CCNGG 1 cut(s) 87
MunI CAATTG 1 cut(s) 1234
Mva1269I GAATGC 1 cut(s) 263
MvaI CCWGG 1 cut(s) 87
MwoI GCNNNNNNNGC 3 cut(s) 313, 422, 655
NcoI CCATGG 1 cut(s) 142
NdeII GATC 5 cut(s) 183, 286, 472, 619, 955
NlaIV GGNNCC 1 cut(s) 403
NmuCI GTSAC 1 cut(s) 1205
NspI RCATGY 1 cut(s) 537
NspV TTCGAA 1 cut(s) 164
PceI AGGCCT 2 cut(s) 442, 729
PctI GAATGC 1 cut(s) 263
PfeI GAWTC 3 cut(s) 62, 322, 1196
PflFI GACNNNGTC 1 cut(s) 1073
PflMI CCANNNNNTGG 1 cut(s) 148
PkrI GCNGC 1 cut(s) 969
Psp6I CCWGG 1 cut(s) 85
PspFI CCCAGC 2 cut(s) 566, 1058
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 1 cut(s) 403
PspPI GGNCC 2 cut(s) 7, 492
PstNI CAGNNNCTG 1 cut(s) 629
PsyI GACNNNGTC 1 cut(s) 1073
PvuII CAGCTG 1 cut(s) 970
RsaI GTAC 1 cut(s) 920
RsaNI GTAC 1 cut(s) 919
RseI CAYNNNNRTG 2 cut(s) 112, 1123
SaqAI TTAA 6 cut(s) 17, 47, 480, 888, 1113, 1121
SatI GCNGC 1 cut(s) 968
Sau3AI GATC 5 cut(s) 183, 286, 472, 619, 955
Sau96I GGNCC 2 cut(s) 7, 492
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 1 cut(s) 785
SfaNI GCATC 2 cut(s) 79, 316
SfuI TTCGAA 1 cut(s) 164
SinI GGWCC 2 cut(s) 7, 492
SmiMI CAYNNNNRTG 2 cut(s) 112, 1123
SmlI CTYRAG 1 cut(s) 1037
SmoI CTYRAG 1 cut(s) 1037
Sse9I AATT 7 cut(s) 19, 53, 393, 701, 821, 1010, 1234
SseBI AGGCCT 2 cut(s) 442, 729
SsiI CCGC 2 cut(s) 330, 520
SspMI CTAG 3 cut(s) 35, 216, 1223
StuI AGGCCT 2 cut(s) 442, 729
StyD4I CCNGG 1 cut(s) 85
StyI CCWWGG 3 cut(s) 142, 1107, 1210
TaaI ACNGT 4 cut(s) 351, 862, 962, 1168
TaiI ACGT 3 cut(s) 173, 577, 798
TaqI TCGA 1 cut(s) 164
TasI AATT 7 cut(s) 19, 53, 393, 701, 821, 1010, 1234
TfiI GAWTC 3 cut(s) 62, 322, 1196
Tru1I TTAA 6 cut(s) 17, 47, 480, 888, 1113, 1121
Tru9I TTAA 6 cut(s) 17, 47, 480, 888, 1113, 1121
TscAI CASTG 3 cut(s) 630, 965, 1171
TseFI GTSAC 1 cut(s) 1205
TseI GCWGC 1 cut(s) 967
Tsp45I GTSAC 1 cut(s) 1205
TspDTI ATGAA 4 cut(s) 351, 695, 1023, 1191
TspGWI ACGGA 2 cut(s) 315, 465
TspRI CASTG 3 cut(s) 630, 965, 1171
Tth111I GACNNNGTC 1 cut(s) 1073
Van91I CCANNNNNTGG 1 cut(s) 148
VneI GTGCAC 1 cut(s) 781
VpaK11BI GGWCC 2 cut(s) 7, 492
XapI RAATTY 3 cut(s) 19, 53, 701
XceI RCATGY 1 cut(s) 537
XmiI GTMKAC 1 cut(s) 857
XspI CTAG 3 cut(s) 35, 216, 1223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.