AT1G18440

Belongs to the PTH family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007236
Physical Location & Seq
Forward (+)
6345974 .. 6347918
1945 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G18440.1

Sequence Viewer

Length: 867 bp
ATGAAAGCTGTAGCTTTTCCCGCTAAAATCGCAAATCTTAGCTTCCCTTCTAACTGTTGCTCACTATTCTTCCGATCACCGGCGACGTTTTTATCGCCGGCATTGCCTTGTCGGAAGCTGACGAAAGGAATAAGAGGTCTCGAAGGCTTGATGTCTCAGTGTTTATCATCATCGTCACAATCATTGTCATTTTCATCGAATTCATTCTCCTCTCAACCGGAATCAGAGTTACTTCAAGCTTTACCTTCTTCGAAGCCAAAATCTCCGCCACTGCAGCTGCCATGGCTCATTGTTGGTTTGGGTAATCCCGGCAAGAAGTACCAGGGCACGCGCCACAACGTTGGTTTTGAGATGGTGGACGCTTTGGCTGATGCAGAAGGCATCTCCATGAACACTGTTAACTTCAAGGCCTTGTTTGGGAAAGGCGTTATTGGAAATATACCGATTATGCTGGCTAAACCACAAACTTTCATGAACCTAAGTGGTGAGTCTGTTGGACAAATCGTTTCATTTTACAAGATCCCGTTAAAGCAAGTACTTGTGGTTTACGATGATTTAGATTTGCCCTTTGGTAAACTACGTTTATTGCCAAAAGGTGGTCACGGAGGCCACAATGGGATGAGAAGTATTATAGACCGCCTCAAAGGTAGCCGTGATTTTCCTCGACTAAGAATAGGGATCGGACGTCCTCCAGGGAAGATGGATACAGCCAATTTTGTTCTCCGCCAGTTCAATAGACAGGAACAGGAAGAGTTGGATCACACATTCCAAACCGGGTTAGAGGCAATAAGGATTCTTTTGCTTGAGGGGTTCAACAAAAGTGCAACATTTGTCAACACTCGAAAATCTATGGAGCAACTCAGTTAG

Protein Analysis

288

Amino Acids

31.56

Weight (kDa)

9.8

Isoelectric Point (pI)

48.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 96 - 276 3e-68 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 688
AccB7I CCANNNNNTGG 1 cut(s) 596
AccII CGCG 1 cut(s) 331
AciI CCGC 4 cut(s) 21, 266, 637, 724
AclI AACGTT 1 cut(s) 339
AclWI GGATC 3 cut(s) 514, 686, 765
AcsI RAATTY 1 cut(s) 199
AcyI GRCGYC 1 cut(s) 685
AfaI GTAC 2 cut(s) 320, 537
AfiI CCNNNNNNNGG 3 cut(s) 79, 417, 596
AgsI TTSAA 4 cut(s) 236, 406, 733, 814
AjnI CCWGG 2 cut(s) 321, 691
AluBI AGCT 6 cut(s) 8, 14, 42, 118, 239, 277
AluI AGCT 6 cut(s) 8, 14, 42, 118, 239, 277
Alw26I GTCTC 2 cut(s) 143, 159
AlwI GGATC 3 cut(s) 514, 686, 765
AoxI GGCC 2 cut(s) 408, 607
ApeKI GCWGC 2 cut(s) 274, 277
ApoI RAATTY 1 cut(s) 199
Asp700I GAANNNNTTC 1 cut(s) 203
AspLEI GCGC 1 cut(s) 333
AsuC2I CCSGG 2 cut(s) 309, 775
AsuHPI GGTGA 2 cut(s) 69, 497
AsuII TTCGAA 1 cut(s) 251
BaeGI GKGCMC 1 cut(s) 329
BbvI GCAGC 2 cut(s) 264, 286
BccI CCATC 2 cut(s) 346, 694
BceAI ACGGC 1 cut(s) 636
BciT130I CCWGG 2 cut(s) 323, 693
BciVI GTATCC 1 cut(s) 697
BcnI CCSGG 2 cut(s) 309, 775
BcoDI GTCTC 2 cut(s) 143, 159
BfmI CTRYAG 2 cut(s) 9, 272
BfuI GTATCC 1 cut(s) 697
BisI GCNGC 2 cut(s) 275, 278
BlsI GCNGC 2 cut(s) 276, 279
BmcAI AGTACT 1 cut(s) 537
Bme1390I CCNGG 4 cut(s) 309, 323, 693, 775
BmrFI CCNGG 4 cut(s) 309, 323, 693, 775
BmsI GCATC 2 cut(s) 361, 390
BpmI CTGGAG 1 cut(s) 675
Bpu14I TTCGAA 1 cut(s) 251
BpuEI CTTGAG 1 cut(s) 824
BpuMI CCSGG 2 cut(s) 309, 775
BsaHI GRCGYC 1 cut(s) 685
BsaI GGTCTC 1 cut(s) 143
BsaJI CCNNGG 3 cut(s) 281, 322, 692
BsaWI WCCGGW 1 cut(s) 217
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 417, 596
Bse118I RCCGGY 2 cut(s) 79, 97
Bse1I ACTGG 1 cut(s) 727
Bse3DI GCAATG 1 cut(s) 101
BseBI CCWGG 2 cut(s) 323, 693
BseDI CCNNGG 3 cut(s) 281, 322, 692
BseGI GGATG 1 cut(s) 624
BseLI CCNNNNNNNGG 3 cut(s) 79, 417, 596
BseMI GCAATG 1 cut(s) 101
BseMII CTCAG 1 cut(s) 170
BseNI ACTGG 1 cut(s) 727
BseRI GAGGAG 1 cut(s) 199
BseSI GKGCMC 1 cut(s) 329
BseXI GCAGC 2 cut(s) 264, 286
Bsh1236I CGCG 1 cut(s) 331
BshFI GGCC 2 cut(s) 410, 609
BsiSI CCGG 5 cut(s) 80, 98, 218, 309, 774
BslI CCNNNNNNNGG 3 cut(s) 79, 417, 596
BsmAI GTCTC 2 cut(s) 143, 159
BsnI GGCC 2 cut(s) 410, 609
Bso31I GGTCTC 1 cut(s) 143
Bsp119I TTCGAA 1 cut(s) 251
Bsp1286I GDGCHC 1 cut(s) 329
Bsp143I GATC 4 cut(s) 74, 519, 678, 757
Bsp19I CCATGG 1 cut(s) 281
BspACI CCGC 4 cut(s) 21, 266, 637, 724
BspANI GGCC 2 cut(s) 410, 609
BspCNI CTCAG 1 cut(s) 169
BspFNI CGCG 1 cut(s) 331
BspHI TCATGA 1 cut(s) 471
BspMAI CTGCAG 1 cut(s) 276
BspPI GGATC 3 cut(s) 514, 686, 765
BspT104I TTCGAA 1 cut(s) 251
BspTNI GGTCTC 1 cut(s) 143
BsrDI GCAATG 1 cut(s) 101
BsrFI RCCGGY 2 cut(s) 79, 97
BsrI ACTGG 1 cut(s) 727
BssAI RCCGGY 2 cut(s) 79, 97
BssECI CCNNGG 3 cut(s) 281, 322, 692
BssMI GATC 4 cut(s) 74, 519, 678, 757
BssNI GRCGYC 1 cut(s) 685
BssT1I CCWWGG 1 cut(s) 281
Bst2UI CCWGG 2 cut(s) 323, 693
Bst4CI ACNGT 2 cut(s) 56, 397
Bst6I CTCTTC 1 cut(s) 744
BstACI GRCGYC 1 cut(s) 685
BstBI TTCGAA 1 cut(s) 251
BstC8I GCNNGC 3 cut(s) 99, 329, 453
BstDEI CTNAG 5 cut(s) 38, 156, 479, 668, 860
BstDSI CCRYGG 1 cut(s) 281
BstF5I GGATG 1 cut(s) 624
BstFNI CGCG 1 cut(s) 331
BstHHI GCGC 1 cut(s) 333
BstKTI GATC 4 cut(s) 77, 522, 681, 760
BstMAI GTCTC 2 cut(s) 143, 159
BstMBI GATC 4 cut(s) 74, 519, 678, 757
BstMWI GCNNNNNNNGC 5 cut(s) 20, 29, 103, 274, 283
BstNI CCWGG 2 cut(s) 323, 693
BstSCI CCNGG 4 cut(s) 307, 321, 691, 773
BstSFI CTRYAG 2 cut(s) 9, 272
BstSLI GKGCMC 1 cut(s) 329
BstUI CGCG 1 cut(s) 331
BstV1I GCAGC 2 cut(s) 264, 286
BstX2I RGATCY 1 cut(s) 519
BstXI CCANNNNNNTGG 1 cut(s) 341
BstYI RGATCY 1 cut(s) 519
BsuI GTATCC 1 cut(s) 697
BsuRI GGCC 2 cut(s) 410, 609
BtgI CCRYGG 1 cut(s) 281
BtsCI GGATG 1 cut(s) 624
BtsI GCAGTG 1 cut(s) 269
BtsIMutI CAGTG 3 cut(s) 164, 269, 393
Cac8I GCNNGC 3 cut(s) 99, 329, 453
CciI TCATGA 1 cut(s) 471
CfoI GCGC 1 cut(s) 333
Cfr10I RCCGGY 2 cut(s) 79, 97
CseI GACGC 1 cut(s) 368
Csp6I GTAC 2 cut(s) 319, 536
CviAII CATG 3 cut(s) 282, 388, 472
CviQI GTAC 2 cut(s) 319, 536
DdeI CTNAG 5 cut(s) 38, 156, 479, 668, 860
DpnI GATC 4 cut(s) 76, 521, 680, 759
DpnII GATC 4 cut(s) 74, 519, 678, 757
Eam1104I CTCTTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 744
EciI GGCGGA 2 cut(s) 255, 713
Eco130I CCWWGG 1 cut(s) 281
Eco147I AGGCCT 1 cut(s) 410
Eco31I GGTCTC 1 cut(s) 143
EcoRI GAATTC 1 cut(s) 199
EcoRII CCWGG 2 cut(s) 321, 691
EcoT14I CCWWGG 1 cut(s) 281
ErhI CCWWGG 1 cut(s) 281
FaeI CATG 3 cut(s) 285, 391, 475
FaiI YATR 7 cut(s) 283, 389, 440, 449, 473, 632, 851
FatI CATG 3 cut(s) 281, 387, 471
FauI CCCGC 1 cut(s) 28
Fnu4HI GCNGC 2 cut(s) 275, 278
FokI GGATG 1 cut(s) 631
Fsp4HI GCNGC 2 cut(s) 275, 278
GlaI GCGC 1 cut(s) 332
GluI GCNGC 2 cut(s) 275, 278
GsuI CTGGAG 1 cut(s) 675
HaeIII GGCC 2 cut(s) 410, 609
HapII CCGG 5 cut(s) 80, 98, 218, 309, 774
HgaI GACGC 1 cut(s) 368
HhaI GCGC 1 cut(s) 333
Hin1I GRCGYC 1 cut(s) 685
Hin1II CATG 3 cut(s) 285, 391, 475
Hin6I GCGC 1 cut(s) 331
HinP1I GCGC 1 cut(s) 331
HincII GTYRAC 2 cut(s) 400, 835
HindII GTYRAC 2 cut(s) 400, 835
HindIII AAGCTT 1 cut(s) 237
HinfI GANTC 3 cut(s) 221, 488, 793
HpaI GTTAAC 1 cut(s) 400
HpaII CCGG 5 cut(s) 80, 98, 218, 309, 774
HphI GGTGA 2 cut(s) 69, 497
Hpy166II GTNNAC 5 cut(s) 358, 400, 547, 575, 835
Hpy188I TCNGA 4 cut(s) 74, 114, 226, 683
Hpy188III TCNNGA 2 cut(s) 140, 472
Hpy8I GTNNAC 5 cut(s) 358, 400, 547, 575, 835
Hpy99I CGWCG 1 cut(s) 88
HpyAV CCTTC 4 cut(s) 57, 137, 255, 371
HpyCH4III ACNGT 2 cut(s) 56, 397
HpyCH4IV ACGT 4 cut(s) 86, 339, 580, 685
HpyCH4V TGCA 3 cut(s) 274, 374, 824
HpyF10VI GCNNNNNNNGC 5 cut(s) 20, 29, 103, 274, 283
HpyF3I CTNAG 5 cut(s) 38, 156, 479, 668, 860
HpySE526I ACGT 4 cut(s) 86, 339, 580, 685
Hsp92I GRCGYC 1 cut(s) 685
Hsp92II CATG 3 cut(s) 285, 391, 475
HspAI GCGC 1 cut(s) 331
KroI GCCGGC 1 cut(s) 97
KroNI GCCGGC 1 cut(s) 99
KspAI GTTAAC 1 cut(s) 400
Kzo9I GATC 4 cut(s) 74, 519, 678, 757
LmnI GCTCC 1 cut(s) 853
Lsp1109I GCAGC 2 cut(s) 264, 286
LweI GCATC 2 cut(s) 361, 390
MaeII ACGT 4 cut(s) 86, 339, 580, 685
MaeIII GTNAC 3 cut(s) 174, 228, 599
MalI GATC 4 cut(s) 76, 521, 680, 759
MboI GATC 4 cut(s) 74, 519, 678, 757
MboII GAAGA 4 cut(s) 61, 240, 709, 761
MflI RGATCY 1 cut(s) 519
MhlI GDGCHC 1 cut(s) 329
MluCI AATT 2 cut(s) 199, 712
MlyI GAGTC 1 cut(s) 497
MmeI TCCRAC 3 cut(s) 92, 475, 735
MnlI CCTC 8 cut(s) 128, 220, 599, 650, 672, 699, 775, 799
MroNI GCCGGC 1 cut(s) 97
MroXI GAANNNNTTC 1 cut(s) 203
MseI TTAA 2 cut(s) 399, 527
MslI CAYNNNNRTG 1 cut(s) 386
MspA1I CMGCKG 1 cut(s) 277
MspI CCGG 5 cut(s) 80, 98, 218, 309, 774
MspR9I CCNGG 4 cut(s) 309, 323, 693, 775
MvaI CCWGG 2 cut(s) 323, 693
MvnI CGCG 1 cut(s) 331
MwoI GCNNNNNNNGC 5 cut(s) 20, 29, 103, 274, 283
NaeI GCCGGC 1 cut(s) 99
NciI CCSGG 2 cut(s) 309, 775
NcoI CCATGG 1 cut(s) 281
NdeII GATC 4 cut(s) 74, 519, 678, 757
NgoMIV GCCGGC 1 cut(s) 97
NlaIII CATG 3 cut(s) 285, 391, 475
NmuCI GTSAC 2 cut(s) 174, 599
NspV TTCGAA 1 cut(s) 251
PagI TCATGA 1 cut(s) 471
PceI AGGCCT 1 cut(s) 410
PdiI GCCGGC 1 cut(s) 99
PdmI GAANNNNTTC 1 cut(s) 203
PfeI GAWTC 2 cut(s) 221, 793
PflMI CCANNNNNTGG 1 cut(s) 596
PkrI GCNGC 2 cut(s) 276, 279
PleI GAGTC 1 cut(s) 496
PpsI GAGTC 1 cut(s) 496
Psp1406I AACGTT 1 cut(s) 339
Psp6I CCWGG 2 cut(s) 321, 691
PspGI CCWGG 2 cut(s) 321, 691
PstI CTGCAG 1 cut(s) 276
PsuI RGATCY 1 cut(s) 519
PvuII CAGCTG 1 cut(s) 277
RsaI GTAC 2 cut(s) 320, 537
RsaNI GTAC 2 cut(s) 319, 536
RseI CAYNNNNRTG 1 cut(s) 386
SaqAI TTAA 2 cut(s) 399, 527
SatI GCNGC 2 cut(s) 275, 278
Sau3AI GATC 4 cut(s) 74, 519, 678, 757
ScaI AGTACT 1 cut(s) 537
SchI GAGTC 1 cut(s) 497
ScrFI CCNGG 4 cut(s) 309, 323, 693, 775
SduI GDGCHC 1 cut(s) 329
SfaNI GCATC 2 cut(s) 361, 390
SfcI CTRYAG 2 cut(s) 9, 272
SfuI TTCGAA 1 cut(s) 251
SgrAI CRCCGGYG 1 cut(s) 79
SmiMI CAYNNNNRTG 1 cut(s) 386
SmlI CTYRAG 1 cut(s) 803
SmoI CTYRAG 1 cut(s) 803
Sse9I AATT 2 cut(s) 199, 712
SseBI AGGCCT 1 cut(s) 410
SsiI CCGC 4 cut(s) 21, 266, 637, 724
StuI AGGCCT 1 cut(s) 410
StyD4I CCNGG 4 cut(s) 307, 321, 691, 773
StyI CCWWGG 1 cut(s) 281
TaaI ACNGT 2 cut(s) 56, 397
TaiI ACGT 4 cut(s) 89, 342, 583, 688
TaqI TCGA 5 cut(s) 141, 197, 251, 664, 841
TasI AATT 2 cut(s) 199, 712
TatI WGTACW 1 cut(s) 535
TfiI GAWTC 2 cut(s) 221, 793
Tru1I TTAA 2 cut(s) 399, 527
Tru9I TTAA 2 cut(s) 399, 527
TscAI CASTG 3 cut(s) 164, 276, 400
TseFI GTSAC 2 cut(s) 174, 599
TseI GCWGC 2 cut(s) 274, 277
Tsp45I GTSAC 2 cut(s) 174, 599
TspDTI ATGAA 7 cut(s) 17, 183, 192, 404, 460, 488, 498
TspGWI ACGGA 1 cut(s) 618
TspRI CASTG 3 cut(s) 164, 276, 400
Van91I CCANNNNNTGG 1 cut(s) 596
XapI RAATTY 1 cut(s) 199
XmnI GAANNNNTTC 1 cut(s) 203
ZraI GACGTC 1 cut(s) 686
ZrmI AGTACT 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.