FvH4_5g12500

Belongs to the PTH family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007236
Physical Location & Seq
Forward (+)
7063846 .. 7066834
2989 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12500.t5

Sequence Viewer

Length: 783 bp
ATGAGTATGAGCGCCGCTGCTTCTATCTCCTCCATCGCCACCCTTCGCTTCCCCACTTGCCGCCCTTCCTCCTGCTTTCGCGCCTCTAAATCTCTCTCGCTTCCCACAATGGCTCAATTCTCCTCCTCCGCCACTTCTCCGCCGCCTTCGACCTCCGACGCGGCGCCCGTCGAAGCCAAGAAGCCGCCCAAGCTGCCGTGGCTCATAGTCGGACTCGGAAACCCCGGCAAGATGTACAGCCGCACCCGCCACAATGTGGGTTTTGAGATGGTGGATGCTATAGCTGAAGCTGAAGGGATAGCTTTGAGCAGTGTTTCTTTCAAAGCCCTTATTGGAAAAGGCTTTATTGGGGATGCCCCAGTTATATTTGCCAAACCGCAAACGTACATGAATAAAAGTGGTGAGTCTGTTGGAGCCATTGTCTCATATTACAAAATTCCATTGCAGCAAGTACTTGTGATATTTGATGATCTGGATCTGCCTTTTGCAAAATTGCGGCTGTTGCCAAAAGGTGGACATGGAGGACAAAATGGGATGAGGAGTATCATTGATCATTTTAAAGGAAGCCGTGACTTTCCACGTTTGAGAATCGGCATTGGCCGGCCTCCTGGGAAAATGAACCCTGCCAGCTATGTTCTTCGTCCATTTACTAAACAAGAACGCGAGGAGTTAGATTTTACATTTCAACACGGCACAGACGCAGTGCGGATCCTTTTGCTTGAAGGGTTCAACAAAGCGGCAACATTTGTTAACACAGCCAAACCCTTGGAGCAATGTGGTTAA

Protein Analysis

261

Amino Acids

28.08

Weight (kDa)

9.71

Isoelectric Point (pI)

48.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 68 - 198 2.4e-51 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 163
AccB7I CCANNNNNTGG 2 cut(s) 256, 512
AccII CGCG 3 cut(s) 81, 161, 663
AclWI GGATC 3 cut(s) 483, 703, 716
AcoI YGGCCR 1 cut(s) 598
AcsI RAATTY 1 cut(s) 435
AcuI CTGAAG 2 cut(s) 306, 312
AcyI GRCGYC 1 cut(s) 164
AdeI CACNNNGTG 1 cut(s) 256
AfaI GTAC 3 cut(s) 236, 386, 453
AfiI CCNNNNNNNGG 2 cut(s) 256, 512
AgsI TTSAA 4 cut(s) 322, 686, 722, 730
AjnI CCWGG 1 cut(s) 607
AluBI AGCT 5 cut(s) 193, 284, 290, 302, 630
AluI AGCT 5 cut(s) 193, 284, 290, 302, 630
Alw26I GTCTC 1 cut(s) 427
AlwI GGATC 3 cut(s) 483, 703, 716
AoxI GGCC 2 cut(s) 598, 602
ApeKI GCWGC 3 cut(s) 17, 193, 445
ApoI RAATTY 1 cut(s) 435
AspLEI GCGC 3 cut(s) 14, 83, 166
AsuC2I CCSGG 1 cut(s) 225
AsuHPI GGTGA 1 cut(s) 413
BamHI GGATCC 1 cut(s) 708
BanI GGYRCC 1 cut(s) 163
BbvI GCAGC 3 cut(s) 4, 180, 457
BccI CCATC 2 cut(s) 41, 262
BceAI ACGGC 3 cut(s) 181, 552, 706
BciT130I CCWGG 1 cut(s) 609
BclI TGATCA 1 cut(s) 550
BcnI CCSGG 1 cut(s) 225
BcoDI GTCTC 1 cut(s) 427
BfmI CTRYAG 1 cut(s) 279
BfoI RGCGCY 2 cut(s) 15, 167
BmcAI AGTACT 1 cut(s) 453
Bme1390I CCNGG 2 cut(s) 225, 609
BmiI GGNNCC 3 cut(s) 165, 415, 710
BmrFI CCNGG 2 cut(s) 225, 609
BmrI ACTGGG 1 cut(s) 353
BmsI GCATC 2 cut(s) 265, 343
BmuI ACTGGG 1 cut(s) 353
BpuMI CCSGG 1 cut(s) 225
BsaBI GATNNNNATC 1 cut(s) 474
BsaHI GRCGYC 1 cut(s) 164
BsaJI CCNNGG 4 cut(s) 197, 223, 608, 765
BsaXI ACNNNNNCTCC 2 cut(s) 405, 435
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 512
Bse118I RCCGGY 1 cut(s) 600
Bse1I ACTGG 1 cut(s) 359
Bse3DI GCAATG 2 cut(s) 440, 779
Bse8I GATNNNNATC 1 cut(s) 474
BseBI CCWGG 1 cut(s) 609
BseDI CCNNGG 4 cut(s) 197, 223, 608, 765
BseGI GGATG 3 cut(s) 280, 358, 540
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 2 cut(s) 256, 512
BseMI GCAATG 2 cut(s) 440, 779
BseNI ACTGG 1 cut(s) 359
BseRI GAGGAG 5 cut(s) 19, 112, 115, 553, 680
BseXI GCAGC 3 cut(s) 4, 180, 457
Bsh1236I CGCG 3 cut(s) 81, 161, 663
BshFI GGCC 2 cut(s) 600, 604
BshNI GGYRCC 1 cut(s) 163
BsiSI CCGG 2 cut(s) 225, 601
BslI CCNNNNNNNGG 2 cut(s) 256, 512
BsmAI GTCTC 1 cut(s) 427
BsnI GGCC 2 cut(s) 600, 604
Bsp1407I TGTACA 1 cut(s) 234
Bsp143I GATC 4 cut(s) 469, 475, 550, 708
BspANI GGCC 2 cut(s) 600, 604
BspFNI CGCG 3 cut(s) 81, 161, 663
BspLI GGNNCC 3 cut(s) 165, 415, 710
BspPI GGATC 3 cut(s) 483, 703, 716
BspT107I GGYRCC 1 cut(s) 163
BsrDI GCAATG 2 cut(s) 440, 779
BsrFI RCCGGY 1 cut(s) 600
BsrGI TGTACA 1 cut(s) 234
BsrI ACTGG 1 cut(s) 359
BssAI RCCGGY 1 cut(s) 600
BssECI CCNNGG 4 cut(s) 197, 223, 608, 765
BssMI GATC 4 cut(s) 469, 475, 550, 708
BssNI GRCGYC 1 cut(s) 164
BssT1I CCWWGG 1 cut(s) 765
Bst2UI CCWGG 1 cut(s) 609
BstACI GRCGYC 1 cut(s) 164
BstAUI TGTACA 1 cut(s) 234
BstC8I GCNNGC 2 cut(s) 602, 628
BstDSI CCRYGG 1 cut(s) 197
BstF5I GGATG 3 cut(s) 280, 358, 540
BstFNI CGCG 3 cut(s) 81, 161, 663
BstH2I RGCGCY 2 cut(s) 15, 167
BstHHI GCGC 3 cut(s) 14, 83, 166
BstKTI GATC 4 cut(s) 472, 478, 553, 711
BstMAI GTCTC 1 cut(s) 427
BstMBI GATC 4 cut(s) 469, 475, 550, 708
BstMWI GCNNNNNNNGC 5 cut(s) 190, 193, 199, 246, 502
BstNI CCWGG 1 cut(s) 609
BstSCI CCNGG 2 cut(s) 223, 607
BstSFI CTRYAG 1 cut(s) 279
BstUI CGCG 3 cut(s) 81, 161, 663
BstV1I GCAGC 3 cut(s) 4, 180, 457
BstX2I RGATCY 2 cut(s) 475, 708
BstXI CCANNNNNNTGG 1 cut(s) 766
BstYI RGATCY 2 cut(s) 475, 708
BsuRI GGCC 2 cut(s) 600, 604
BtgI CCRYGG 1 cut(s) 197
BtgZI GCGATG 1 cut(s) 19
BtsCI GGATG 3 cut(s) 280, 358, 540
BtsI GCAGTG 2 cut(s) 316, 708
BtsIMutI CAGTG 2 cut(s) 316, 708
Cac8I GCNNGC 2 cut(s) 602, 628
CfoI GCGC 3 cut(s) 14, 83, 166
Cfr10I RCCGGY 1 cut(s) 600
CseI GACGC 2 cut(s) 167, 707
Csp6I GTAC 3 cut(s) 235, 385, 452
CviAII CATG 2 cut(s) 388, 518
CviQI GTAC 3 cut(s) 235, 385, 452
DinI GGCGCC 1 cut(s) 165
DpnI GATC 4 cut(s) 471, 477, 552, 710
DpnII GATC 4 cut(s) 469, 475, 550, 708
DraI TTTAAA 1 cut(s) 559
DraIII CACNNNGTG 1 cut(s) 256
EaeI YGGCCR 1 cut(s) 598
EciI GGCGGA 2 cut(s) 118, 129
Eco130I CCWWGG 1 cut(s) 765
Eco57I CTGAAG 2 cut(s) 306, 312
EcoRII CCWGG 1 cut(s) 607
EcoT14I CCWWGG 1 cut(s) 765
EgeI GGCGCC 1 cut(s) 165
EheI GGCGCC 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 765
FaeI CATG 2 cut(s) 391, 521
FaiI YATR 8 cut(s) 8, 206, 281, 365, 389, 427, 519, 633
FatI CATG 2 cut(s) 387, 517
FauI CCCGC 1 cut(s) 254
FbaI TGATCA 1 cut(s) 550
FokI GGATG 3 cut(s) 287, 365, 547
FseI GGCCGGCC 1 cut(s) 604
GlaI GCGC 3 cut(s) 13, 82, 165
HaeII RGCGCY 2 cut(s) 15, 167
HaeIII GGCC 2 cut(s) 600, 604
HapII CCGG 2 cut(s) 225, 601
HgaI GACGC 2 cut(s) 167, 707
HhaI GCGC 3 cut(s) 14, 83, 166
Hin1I GRCGYC 1 cut(s) 164
Hin1II CATG 2 cut(s) 391, 521
Hin6I GCGC 3 cut(s) 12, 81, 164
HinP1I GCGC 3 cut(s) 12, 81, 164
HincII GTYRAC 1 cut(s) 751
HindII GTYRAC 1 cut(s) 751
HinfI GANTC 3 cut(s) 213, 404, 588
HpaI GTTAAC 1 cut(s) 751
HpaII CCGG 2 cut(s) 225, 601
HphI GGTGA 1 cut(s) 413
Hpy166II GTNNAC 2 cut(s) 515, 751
Hpy188I TCNGA 3 cut(s) 157, 212, 218
Hpy188III TCNNGA 1 cut(s) 473
Hpy8I GTNNAC 2 cut(s) 515, 751
Hpy99I CGWCG 2 cut(s) 161, 173
HpyAV CCTTC 5 cut(s) 53, 75, 156, 287, 716
HpyCH4IV ACGT 2 cut(s) 383, 580
HpyCH4V TGCA 2 cut(s) 445, 488
HpyF10VI GCNNNNNNNGC 5 cut(s) 190, 193, 199, 246, 502
HpySE526I ACGT 2 cut(s) 383, 580
Hsp92I GRCGYC 1 cut(s) 164
Hsp92II CATG 2 cut(s) 391, 521
HspAI GCGC 3 cut(s) 12, 81, 164
KasI GGCGCC 1 cut(s) 163
KroI GCCGGC 1 cut(s) 600
KroNI GCCGGC 1 cut(s) 602
Ksp22I TGATCA 1 cut(s) 550
KspAI GTTAAC 1 cut(s) 751
Kzo9I GATC 4 cut(s) 469, 475, 550, 708
LmnI GCTCC 2 cut(s) 413, 769
LpnPI CCDG 9 cut(s) 85, 238, 372, 458, 594, 614, 621, 636, 640
Lsp1109I GCAGC 3 cut(s) 4, 180, 457
LweI GCATC 2 cut(s) 265, 343
MaeII ACGT 2 cut(s) 383, 580
MaeIII GTNAC 1 cut(s) 569
MalI GATC 4 cut(s) 471, 477, 552, 710
MboI GATC 4 cut(s) 469, 475, 550, 708
MboII GAAGA 1 cut(s) 629
MflI RGATCY 2 cut(s) 475, 708
MluCI AATT 3 cut(s) 116, 435, 491
Mly113I GGCGCC 1 cut(s) 164
MlyI GAGTC 2 cut(s) 207, 413
MmeI TCCRAC 3 cut(s) 180, 190, 391
MroNI GCCGGC 1 cut(s) 600
MseI TTAA 3 cut(s) 558, 750, 781
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 2 cut(s) 225, 601
MspR9I CCNGG 2 cut(s) 225, 609
MvaI CCWGG 1 cut(s) 609
MvnI CGCG 3 cut(s) 81, 161, 663
MwoI GCNNNNNNNGC 5 cut(s) 190, 193, 199, 246, 502
NaeI GCCGGC 1 cut(s) 602
NarI GGCGCC 1 cut(s) 164
NciI CCSGG 1 cut(s) 225
NdeII GATC 4 cut(s) 469, 475, 550, 708
NgoMIV GCCGGC 1 cut(s) 600
NlaIII CATG 2 cut(s) 391, 521
NlaIV GGNNCC 3 cut(s) 165, 415, 710
NmuCI GTSAC 1 cut(s) 569
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PdiI GCCGGC 1 cut(s) 602
PfeI GAWTC 1 cut(s) 588
PflMI CCANNNNNTGG 2 cut(s) 256, 512
PleI GAGTC 2 cut(s) 207, 412
PluTI GGCGCC 1 cut(s) 167
PpsI GAGTC 2 cut(s) 207, 412
Psp6I CCWGG 1 cut(s) 607
PspGI CCWGG 1 cut(s) 607
PspN4I GGNNCC 3 cut(s) 165, 415, 710
PsuI RGATCY 2 cut(s) 475, 708
RigI GGCCGGCC 1 cut(s) 604
RsaI GTAC 3 cut(s) 236, 386, 453
RsaNI GTAC 3 cut(s) 235, 385, 452
SaqAI TTAA 3 cut(s) 558, 750, 781
Sau3AI GATC 4 cut(s) 469, 475, 550, 708
ScaI AGTACT 1 cut(s) 453
SchI GAGTC 2 cut(s) 207, 413
ScrFI CCNGG 2 cut(s) 225, 609
SetI ASST 9 cut(s) 155, 195, 286, 292, 304, 386, 514, 583, 632
SfaNI GCATC 2 cut(s) 265, 343
SfcI CTRYAG 1 cut(s) 279
SfoI GGCGCC 1 cut(s) 165
Sse9I AATT 3 cut(s) 116, 435, 491
SspDI GGCGCC 1 cut(s) 163
StyD4I CCNGG 2 cut(s) 223, 607
StyI CCWWGG 1 cut(s) 765
TaiI ACGT 2 cut(s) 386, 583
TaqI TCGA 2 cut(s) 149, 171
TasI AATT 3 cut(s) 116, 435, 491
TatI WGTACW 2 cut(s) 234, 451
TauI GCSGC 8 cut(s) 17, 63, 145, 164, 187, 243, 499, 740
TfiI GAWTC 1 cut(s) 588
Tru1I TTAA 3 cut(s) 558, 750, 781
Tru9I TTAA 3 cut(s) 558, 750, 781
TscAI CASTG 2 cut(s) 316, 708
TseFI GTSAC 1 cut(s) 569
TseI GCWGC 3 cut(s) 17, 193, 445
Tsp45I GTSAC 1 cut(s) 569
TspDTI ATGAA 2 cut(s) 404, 632
TspRI CASTG 2 cut(s) 316, 708
Van91I CCANNNNNTGG 2 cut(s) 256, 512
XapI RAATTY 1 cut(s) 435
ZrmI AGTACT 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.