MD06G1212500.v1.1

Belongs to the PTH family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007236
Physical Location & Seq
Reverse (-)
34520919 .. 34523149
2231 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1212500.v1.1.491

Sequence Viewer

Length: 780 bp
ATGAACGTTGCTGCTTCCCTATCATCCATCGCCACCGTCCGCTTCCCCAATTGCCACCGCTGCTTCGGCTTCTCAAAACCCCTAATTCAATCTCCGTCGCTTTGGATAATGGCCCAATTCTCGTCATCGCAATCTCCCTCCGCCGCGCCGGAAACGGCGGTTGTGGAAGCCAAGACGCCGCCCAAGCAGCCGTGGCTCATCGTCGGCCTCGGAAACCCCGGGAAGAAGTACAACAGCACCCGCCACAATGTGGGTTTTGAGATGGTGGATGCCATTGCTGAAGCTGAAGGGATACCTTTGAGCAGTGTTTCTTTCAAAGCCCTTGTTGGAAAAGGTCTTATTGGGGATGTTCCGGTTATATTTGCCAAACCACAAACTTACATGAATAAAAGCGGTGAGTCTGTTGGATCCATTGTTTCGTATTACAAGATTCCATTGAAGCAAGTACTTGTGAGTATATTTGATGATCTAGATCTTCCCTTTGGAAAACTGCGGATGTTGCCGAAAGGTGGACATGGAGGACACAATGGGATGAGGAGTGTCATTGATCACTTTAAAGGGAGTCGAGATTTTCCTCGTTTAAGAATCGGCATTGGACGCCCTCCTGGGAAAATGGATCCTATCAACTTTGTTCTTCGTCCGTTCACTAAACAAGAACAACAGGAGCTGAATTTTACGTTTCAAGAAGGTGTAGAAGCGGTGCGTATTCTTTTGCTTGAGGGGTTCAACAAAAGCGCAACTTTTGTTAACAGTGCCAAACCCTTGGAACAATGCGGTTAA

Protein Analysis

260

Amino Acids

28.23

Weight (kDa)

9.54

Isoelectric Point (pI)

46.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 66 - 247 1.1e-63 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 250
AccII CGCG 1 cut(s) 146
AclI AACGTT 1 cut(s) 6
AclWI GGATC 4 cut(s) 402, 415, 611, 624
AcsI RAATTY 1 cut(s) 670
AcuI CTGAAG 2 cut(s) 300, 306
AcyI GRCGYC 2 cut(s) 176, 598
AdeI CACNNNGTG 1 cut(s) 250
AfaI GTAC 2 cut(s) 230, 447
AfiI CCNNNNNNNGG 2 cut(s) 250, 509
AgsI TTSAA 5 cut(s) 89, 316, 439, 683, 727
AjnI CCWGG 1 cut(s) 604
AluBI AGCT 2 cut(s) 284, 667
AluI AGCT 2 cut(s) 284, 667
AlwI GGATC 4 cut(s) 402, 415, 611, 624
AlwNI CAGNNNCTG 1 cut(s) 667
Ama87I CYCGRG 1 cut(s) 218
AoxI GGCC 2 cut(s) 111, 205
ApeKI GCWGC 3 cut(s) 11, 60, 187
ApoI RAATTY 1 cut(s) 670
AspLEI GCGC 2 cut(s) 148, 737
AspS9I GGNCC 1 cut(s) 112
AsuC2I CCSGG 2 cut(s) 219, 220
AsuHPI GGTGA 1 cut(s) 407
AvaI CYCGRG 1 cut(s) 218
BamHI GGATCC 2 cut(s) 407, 616
BbvI GCAGC 2 cut(s) 47, 199
BccI CCATC 2 cut(s) 35, 256
BceAI ACGGC 2 cut(s) 171, 175
BciT130I CCWGG 1 cut(s) 606
BciVI GTATCC 1 cut(s) 285
BclI TGATCA 1 cut(s) 547
BcnI CCSGG 2 cut(s) 219, 220
BfaI CTAG 1 cut(s) 470
BfuI GTATCC 1 cut(s) 285
BglII AGATCT 1 cut(s) 472
BisI GCNGC 5 cut(s) 12, 61, 144, 179, 188
BlsI GCNGC 5 cut(s) 13, 62, 145, 180, 189
BmcAI AGTACT 1 cut(s) 447
Bme1390I CCNGG 3 cut(s) 219, 220, 606
BmeT110I CYCGRG 1 cut(s) 218
BmgT120I GGNCC 1 cut(s) 112
BmiI GGNNCC 2 cut(s) 409, 618
BmrFI CCNGG 3 cut(s) 219, 220, 606
BmsI GCATC 1 cut(s) 259
BpuEI CTTGAG 1 cut(s) 737
BpuMI CCSGG 2 cut(s) 219, 220
BsaBI GATNNNNATC 1 cut(s) 471
BsaHI GRCGYC 2 cut(s) 176, 598
BsaJI CCNNGG 6 cut(s) 191, 208, 217, 218, 605, 762
BsaWI WCCGGW 1 cut(s) 352
Bsc4I CCNNNNNNNGG 2 cut(s) 250, 509
Bse3DI GCAATG 1 cut(s) 273
Bse8I GATNNNNATC 1 cut(s) 471
BseBI CCWGG 1 cut(s) 606
BseDI CCNNGG 6 cut(s) 191, 208, 217, 218, 605, 762
BseGI GGATG 5 cut(s) 23, 274, 352, 501, 537
BseJI GATNNNNATC 1 cut(s) 471
BseLI CCNNNNNNNGG 2 cut(s) 250, 509
BseMI GCAATG 1 cut(s) 273
BseRI GAGGAG 1 cut(s) 550
BseXI GCAGC 2 cut(s) 47, 199
Bsh1236I CGCG 1 cut(s) 146
BshFI GGCC 2 cut(s) 113, 207
BsiHKCI CYCGRG 1 cut(s) 218
BsiSI CCGG 3 cut(s) 149, 219, 353
BslI CCNNNNNNNGG 2 cut(s) 250, 509
BsnI GGCC 2 cut(s) 113, 207
BsoBI CYCGRG 1 cut(s) 218
Bsp143I GATC 5 cut(s) 407, 466, 472, 547, 616
BspANI GGCC 2 cut(s) 113, 207
BspFNI CGCG 1 cut(s) 146
BspLI GGNNCC 2 cut(s) 409, 618
BspPI GGATC 4 cut(s) 402, 415, 611, 624
BsrDI GCAATG 1 cut(s) 273
BssECI CCNNGG 6 cut(s) 191, 208, 217, 218, 605, 762
BssMI GATC 5 cut(s) 407, 466, 472, 547, 616
BssNI GRCGYC 2 cut(s) 176, 598
BssT1I CCWWGG 1 cut(s) 762
Bst2UI CCWGG 1 cut(s) 606
Bst4CI ACNGT 2 cut(s) 37, 752
BstACI GRCGYC 2 cut(s) 176, 598
BstDSI CCRYGG 1 cut(s) 191
BstF5I GGATG 5 cut(s) 23, 274, 352, 501, 537
BstFNI CGCG 1 cut(s) 146
BstHHI GCGC 2 cut(s) 148, 737
BstKTI GATC 5 cut(s) 410, 469, 475, 550, 619
BstMBI GATC 5 cut(s) 407, 466, 472, 547, 616
BstMWI GCNNNNNNNGC 7 cut(s) 60, 66, 184, 187, 193, 499, 597
BstNI CCWGG 1 cut(s) 606
BstSCI CCNGG 3 cut(s) 217, 218, 604
BstUI CGCG 1 cut(s) 146
BstV1I GCAGC 2 cut(s) 47, 199
BstX2I RGATCY 3 cut(s) 407, 472, 616
BstXI CCANNNNNNTGG 1 cut(s) 763
BstYI RGATCY 3 cut(s) 407, 472, 616
BsuI GTATCC 1 cut(s) 285
BsuRI GGCC 2 cut(s) 113, 207
BtgI CCRYGG 1 cut(s) 191
BtgZI GCGATG 2 cut(s) 13, 111
BtsCI GGATG 5 cut(s) 23, 274, 352, 501, 537
BtsI GCAGTG 1 cut(s) 310
BtsIMutI CAGTG 2 cut(s) 310, 757
CaiI CAGNNNCTG 1 cut(s) 667
CfoI GCGC 2 cut(s) 148, 737
Cfr13I GGNCC 1 cut(s) 112
Cfr9I CCCGGG 1 cut(s) 218
CseI GACGC 2 cut(s) 184, 606
Csp6I GTAC 2 cut(s) 229, 446
CviAII CATG 2 cut(s) 382, 515
CviJI RGCY 9 cut(s) 69, 113, 170, 190, 196, 207, 284, 320, 667
CviKI_1 RGCY 9 cut(s) 69, 113, 170, 190, 196, 207, 284, 320, 667
CviQI GTAC 2 cut(s) 229, 446
DpnI GATC 5 cut(s) 409, 468, 474, 549, 618
DpnII GATC 5 cut(s) 407, 466, 472, 547, 616
DraI TTTAAA 1 cut(s) 556
DraIII CACNNNGTG 1 cut(s) 250
EciI GGCGGA 1 cut(s) 130
Eco130I CCWWGG 1 cut(s) 762
Eco57I CTGAAG 2 cut(s) 300, 306
Eco88I CYCGRG 1 cut(s) 218
EcoRII CCWGG 1 cut(s) 604
EcoT14I CCWWGG 1 cut(s) 762
ErhI CCWWGG 1 cut(s) 762
FaeI CATG 2 cut(s) 385, 518
FaiI YATR 4 cut(s) 359, 383, 458, 516
FalI AAGNNNNNCTT 2 cut(s) 724, 756
FatI CATG 2 cut(s) 381, 514
FauI CCCGC 1 cut(s) 248
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 5 cut(s) 12, 61, 144, 179, 188
FokI GGATG 5 cut(s) 10, 281, 359, 508, 544
Fsp4HI GCNGC 5 cut(s) 12, 61, 144, 179, 188
FspBI CTAG 1 cut(s) 470
GlaI GCGC 2 cut(s) 147, 736
GluI GCNGC 5 cut(s) 12, 61, 144, 179, 188
HaeIII GGCC 2 cut(s) 113, 207
HapII CCGG 3 cut(s) 149, 219, 353
HgaI GACGC 2 cut(s) 184, 606
HhaI GCGC 2 cut(s) 148, 737
Hin1I GRCGYC 2 cut(s) 176, 598
Hin1II CATG 2 cut(s) 385, 518
Hin6I GCGC 2 cut(s) 146, 735
HinP1I GCGC 2 cut(s) 146, 735
HincII GTYRAC 1 cut(s) 748
HindII GTYRAC 1 cut(s) 748
HinfI GANTC 4 cut(s) 398, 430, 562, 585
HpaI GTTAAC 1 cut(s) 748
HpaII CCGG 3 cut(s) 149, 219, 353
HphI GGTGA 1 cut(s) 407
Hpy166II GTNNAC 3 cut(s) 512, 645, 748
Hpy188I TCNGA 1 cut(s) 212
Hpy188III TCNNGA 3 cut(s) 470, 566, 683
Hpy8I GTNNAC 3 cut(s) 512, 645, 748
Hpy99I CGWCG 2 cut(s) 100, 206
HpyAV CCTTC 2 cut(s) 281, 680
HpyCH4III ACNGT 2 cut(s) 37, 752
HpyCH4IV ACGT 2 cut(s) 6, 677
HpyF10VI GCNNNNNNNGC 7 cut(s) 60, 66, 184, 187, 193, 499, 597
HpySE526I ACGT 2 cut(s) 6, 677
Hsp92I GRCGYC 2 cut(s) 176, 598
Hsp92II CATG 2 cut(s) 385, 518
HspAI GCGC 2 cut(s) 146, 735
Ksp22I TGATCA 1 cut(s) 547
KspAI GTTAAC 1 cut(s) 748
Kzo9I GATC 5 cut(s) 407, 466, 472, 547, 616
LmnI GCTCC 1 cut(s) 664
LpnPI CCDG 6 cut(s) 162, 232, 366, 591, 618, 647
Lsp1109I GCAGC 2 cut(s) 47, 199
LweI GCATC 1 cut(s) 259
MaeI CTAG 1 cut(s) 470
MaeII ACGT 2 cut(s) 6, 677
MalI GATC 5 cut(s) 409, 468, 474, 549, 618
MboI GATC 5 cut(s) 407, 466, 472, 547, 616
MboII GAAGA 3 cut(s) 235, 467, 626
MfeI CAATTG 1 cut(s) 49
MflI RGATCY 3 cut(s) 407, 472, 616
MluCI AATT 4 cut(s) 49, 84, 116, 670
MlyI GAGTC 2 cut(s) 407, 571
MmeI TCCRAC 2 cut(s) 307, 385
MnlI CCTC 7 cut(s) 148, 218, 512, 528, 585, 612, 712
MseI TTAA 4 cut(s) 555, 581, 747, 778
MspA1I CMGCKG 1 cut(s) 60
MspI CCGG 3 cut(s) 149, 219, 353
MspR9I CCNGG 3 cut(s) 219, 220, 606
MunI CAATTG 1 cut(s) 49
MvaI CCWGG 1 cut(s) 606
MvnI CGCG 1 cut(s) 146
MwoI GCNNNNNNNGC 7 cut(s) 60, 66, 184, 187, 193, 499, 597
NciI CCSGG 2 cut(s) 219, 220
NdeII GATC 5 cut(s) 407, 466, 472, 547, 616
NlaIII CATG 2 cut(s) 385, 518
NlaIV GGNNCC 2 cut(s) 409, 618
PfeI GAWTC 2 cut(s) 430, 585
PflMI CCANNNNNTGG 1 cut(s) 250
PkrI GCNGC 5 cut(s) 13, 62, 145, 180, 189
PleI GAGTC 2 cut(s) 406, 570
PpsI GAGTC 2 cut(s) 406, 570
Psp1406I AACGTT 1 cut(s) 6
Psp6I CCWGG 1 cut(s) 604
PspGI CCWGG 1 cut(s) 604
PspN4I GGNNCC 2 cut(s) 409, 618
PspPI GGNCC 1 cut(s) 112
PstNI CAGNNNCTG 1 cut(s) 667
PsuI RGATCY 3 cut(s) 407, 472, 616
RsaI GTAC 2 cut(s) 230, 447
RsaNI GTAC 2 cut(s) 229, 446
SaqAI TTAA 4 cut(s) 555, 581, 747, 778
SatI GCNGC 5 cut(s) 12, 61, 144, 179, 188
Sau3AI GATC 5 cut(s) 407, 466, 472, 547, 616
Sau96I GGNCC 1 cut(s) 112
ScaI AGTACT 1 cut(s) 447
SchI GAGTC 2 cut(s) 407, 571
ScrFI CCNGG 3 cut(s) 219, 220, 606
SetI ASST 8 cut(s) 9, 286, 298, 337, 511, 669, 680, 691
SfaNI GCATC 1 cut(s) 259
SmaI CCCGGG 1 cut(s) 220
SmlI CTYRAG 1 cut(s) 716
SmoI CTYRAG 1 cut(s) 716
Sse9I AATT 4 cut(s) 49, 84, 116, 670
SspMI CTAG 1 cut(s) 470
StyD4I CCNGG 3 cut(s) 217, 218, 604
StyI CCWWGG 1 cut(s) 762
TaaI ACNGT 2 cut(s) 37, 752
TaiI ACGT 2 cut(s) 9, 680
TaqI TCGA 1 cut(s) 565
TasI AATT 4 cut(s) 49, 84, 116, 670
TatI WGTACW 2 cut(s) 228, 445
TauI GCSGC 2 cut(s) 146, 181
TfiI GAWTC 2 cut(s) 430, 585
Tru1I TTAA 4 cut(s) 555, 581, 747, 778
Tru9I TTAA 4 cut(s) 555, 581, 747, 778
TscAI CASTG 2 cut(s) 310, 757
TseI GCWGC 3 cut(s) 11, 60, 187
TspDTI ATGAA 2 cut(s) 17, 398
TspGWI ACGGA 2 cut(s) 84, 630
TspMI CCCGGG 1 cut(s) 218
TspRI CASTG 2 cut(s) 310, 757
Van91I CCANNNNNTGG 1 cut(s) 250
XapI RAATTY 1 cut(s) 670
XbaI TCTAGA 1 cut(s) 469
XmaI CCCGGG 1 cut(s) 218
XspI CTAG 1 cut(s) 470
ZrmI AGTACT 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.