MD14G1223800.v1.1

Belongs to the PTH family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007236
Physical Location & Seq
Reverse (-)
30481531 .. 30483703
2173 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1223800.v1.1.491

Sequence Viewer

Length: 795 bp
ATGAACGTTGCTGCTTCCCTATCATCCATCGCCACCGTCCGCTTTCCCAATTGCCACCGCTGCTTCCGCTTCTCAAAACCCCTACTTCGATCTCCGTCGCTTCAGATAATGTCCCGAATCTCGTCATCACCATCTCCCTCCACCGTATCCGCGGCGCCAGTGAGCTCGGACACGGCGCCTGTCGAAGCCAAGAAGCCGCCCAAGCAGCCGTGGCTCATCGTCGGACTCGGAAACCCCGGGAAGAAGTACAACAGCACCCGCCACAATGTGGGTTTTGAGATGGTAGATGCCATAGCTGAAGCTGAAGGGATACCTTTGAGCAGTGTTTCTTTCAAAGCCCTTGTTGGAAAAGGCCTTATTGGCGATGTTCCGGTTATATTTGCCAAGCCACAAACTTACATGAATAAAAGCGGTGAGTCTGTTGGATCGATTGTTTCATATTACAAGATTCCATTGAAGCAAGTACTTGTGATATTTGATGATCTAGATCTTCCCTTTGGAAAGCTGCGGATGTTGCCAAAAGGTGGACACGGAGGACACAATGGGATGAGGAGTATCATTGATCACTTTAAAGGGAGTCGAGATTTTCCTCGTTTAAGAATCGGCATTGGACGTCCTCCTGGGAAAATGGATCCTGTCAACTTTGTTCTTCGTCCATTTACTAATCAAGAACAACACGAGTTGAATTTTACGTTTCAAGATGGTGTAGAAGCGGTGCGTATTCTTTTGCTCGAGGGGTTCAACAAAAGCGCAACATTCGTTAATACTGCCAAACCCTTGGAACAATGCGGTTAA

Protein Analysis

265

Amino Acids

28.76

Weight (kDa)

9.79

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 72 - 252 6.6e-66 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 616
AccB1I GGYRCC 2 cut(s) 154, 175
AccB7I CCANNNNNTGG 2 cut(s) 268, 524
AccII CGCG 1 cut(s) 152
AclI AACGTT 1 cut(s) 6
AclWI GGATC 3 cut(s) 433, 626, 639
AcsI RAATTY 1 cut(s) 685
AcuI CTGAAG 3 cut(s) 86, 318, 324
AcyI GRCGYC 3 cut(s) 155, 176, 613
AdeI CACNNNGTG 1 cut(s) 268
AfaI GTAC 2 cut(s) 248, 465
AfiI CCNNNNNNNGG 2 cut(s) 268, 524
AgsI TTSAA 5 cut(s) 334, 457, 685, 698, 742
AjnI CCWGG 1 cut(s) 619
AluBI AGCT 4 cut(s) 165, 296, 302, 505
AluI AGCT 4 cut(s) 165, 296, 302, 505
Alw21I GWGCWC 1 cut(s) 167
AlwI GGATC 3 cut(s) 433, 626, 639
Ama87I CYCGRG 2 cut(s) 236, 731
AoxI GGCC 1 cut(s) 352
ApeKI GCWGC 4 cut(s) 11, 60, 205, 505
ApoI RAATTY 1 cut(s) 685
AspLEI GCGC 3 cut(s) 157, 178, 752
AsuC2I CCSGG 2 cut(s) 237, 238
AsuHPI GGTGA 2 cut(s) 120, 425
AvaI CYCGRG 2 cut(s) 236, 731
BamHI GGATCC 1 cut(s) 631
BanI GGYRCC 2 cut(s) 154, 175
BanII GRGCYC 1 cut(s) 167
BauI CACGAG 1 cut(s) 677
Bbv12I GWGCWC 1 cut(s) 167
BbvI GCAGC 3 cut(s) 47, 217, 492
BccI CCATC 4 cut(s) 35, 139, 274, 695
BceAI ACGGC 2 cut(s) 189, 193
BciT130I CCWGG 1 cut(s) 621
BciVI GTATCC 2 cut(s) 157, 303
BclI TGATCA 1 cut(s) 562
BcnI CCSGG 2 cut(s) 237, 238
BfaI CTAG 1 cut(s) 485
BfoI RGCGCY 2 cut(s) 158, 179
BfuI GTATCC 2 cut(s) 157, 303
BglI GCCNNNNNGGC 1 cut(s) 360
BglII AGATCT 1 cut(s) 487
BisI GCNGC 6 cut(s) 12, 61, 153, 197, 206, 506
BlsI GCNGC 6 cut(s) 13, 62, 154, 198, 207, 507
BmcAI AGTACT 1 cut(s) 465
Bme1390I CCNGG 3 cut(s) 237, 238, 621
BmeT110I CYCGRG 2 cut(s) 236, 731
BmiI GGNNCC 3 cut(s) 156, 177, 633
BmrFI CCNGG 3 cut(s) 237, 238, 621
BmsI GCATC 1 cut(s) 277
BpuMI CCSGG 2 cut(s) 237, 238
Bsa29I ATCGAT 1 cut(s) 428
BsaBI GATNNNNATC 1 cut(s) 486
BsaHI GRCGYC 3 cut(s) 155, 176, 613
BsaJI CCNNGG 6 cut(s) 150, 209, 235, 236, 620, 777
BsaWI WCCGGW 1 cut(s) 370
Bsc4I CCNNNNNNNGG 2 cut(s) 268, 524
Bse1I ACTGG 1 cut(s) 158
Bse8I GATNNNNATC 1 cut(s) 486
BseBI CCWGG 1 cut(s) 621
BseCI ATCGAT 1 cut(s) 428
BseDI CCNNGG 6 cut(s) 150, 209, 235, 236, 620, 777
BseGI GGATG 3 cut(s) 23, 516, 552
BseJI GATNNNNATC 1 cut(s) 486
BseLI CCNNNNNNNGG 2 cut(s) 268, 524
BseNI ACTGG 1 cut(s) 158
BseRI GAGGAG 1 cut(s) 565
BseXI GCAGC 3 cut(s) 47, 217, 492
Bsh1236I CGCG 1 cut(s) 152
BshFI GGCC 1 cut(s) 354
BshNI GGYRCC 2 cut(s) 154, 175
BshVI ATCGAT 1 cut(s) 428
BsiHKAI GWGCWC 1 cut(s) 167
BsiHKCI CYCGRG 2 cut(s) 236, 731
BsiSI CCGG 2 cut(s) 237, 371
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 2 cut(s) 268, 524
BsmFI GGGAC 1 cut(s) 97
BsnI GGCC 1 cut(s) 354
BsoBI CYCGRG 2 cut(s) 236, 731
Bsp1286I GDGCHC 1 cut(s) 167
Bsp143I GATC 6 cut(s) 89, 425, 481, 487, 562, 631
BspANI GGCC 1 cut(s) 354
BspDI ATCGAT 1 cut(s) 428
BspFNI CGCG 1 cut(s) 152
BspLI GGNNCC 3 cut(s) 156, 177, 633
BspPI GGATC 3 cut(s) 433, 626, 639
BspT107I GGYRCC 2 cut(s) 154, 175
BsrI ACTGG 1 cut(s) 158
BssECI CCNNGG 6 cut(s) 150, 209, 235, 236, 620, 777
BssMI GATC 6 cut(s) 89, 425, 481, 487, 562, 631
BssNI GRCGYC 3 cut(s) 155, 176, 613
BssSI CACGAG 1 cut(s) 677
BssT1I CCWWGG 1 cut(s) 777
Bst2BI CACGAG 1 cut(s) 677
Bst2UI CCWGG 1 cut(s) 621
Bst4CI ACNGT 2 cut(s) 37, 145
BstACI GRCGYC 3 cut(s) 155, 176, 613
BstDSI CCRYGG 2 cut(s) 150, 209
BstF5I GGATG 3 cut(s) 23, 516, 552
BstFNI CGCG 1 cut(s) 152
BstH2I RGCGCY 2 cut(s) 158, 179
BstHHI GCGC 3 cut(s) 157, 178, 752
BstKTI GATC 6 cut(s) 92, 428, 484, 490, 565, 634
BstMBI GATC 6 cut(s) 89, 425, 481, 487, 562, 631
BstMWI GCNNNNNNNGC 7 cut(s) 60, 66, 202, 205, 211, 360, 514
BstNI CCWGG 1 cut(s) 621
BstSCI CCNGG 3 cut(s) 235, 236, 619
BstUI CGCG 1 cut(s) 152
BstV1I GCAGC 3 cut(s) 47, 217, 492
BstX2I RGATCY 2 cut(s) 487, 631
BstXI CCANNNNNNTGG 1 cut(s) 778
BstYI RGATCY 2 cut(s) 487, 631
Bsu15I ATCGAT 1 cut(s) 428
BsuI GTATCC 2 cut(s) 157, 303
BsuRI GGCC 1 cut(s) 354
BsuTUI ATCGAT 1 cut(s) 428
BtgI CCRYGG 2 cut(s) 150, 209
BtgZI GCGATG 2 cut(s) 13, 378
BtsCI GGATG 3 cut(s) 23, 516, 552
BtsI GCAGTG 1 cut(s) 328
BtsIMutI CAGTG 2 cut(s) 165, 328
CfoI GCGC 3 cut(s) 157, 178, 752
Cfr42I CCGCGG 1 cut(s) 153
Cfr9I CCCGGG 1 cut(s) 236
ClaI ATCGAT 1 cut(s) 428
Csp6I GTAC 2 cut(s) 247, 464
CviAII CATG 1 cut(s) 400
CviQI GTAC 2 cut(s) 247, 464
DinI GGCGCC 2 cut(s) 156, 177
DpnI GATC 6 cut(s) 91, 427, 483, 489, 564, 633
DpnII GATC 6 cut(s) 89, 425, 481, 487, 562, 631
DraI TTTAAA 1 cut(s) 571
DraIII CACNNNGTG 1 cut(s) 268
Ecl136II GAGCTC 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 777
Eco147I AGGCCT 1 cut(s) 354
Eco24I GRGCYC 1 cut(s) 167
Eco53kI GAGCTC 1 cut(s) 165
Eco57I CTGAAG 3 cut(s) 86, 318, 324
Eco88I CYCGRG 2 cut(s) 236, 731
EcoICRI GAGCTC 1 cut(s) 165
EcoRII CCWGG 1 cut(s) 619
EcoT14I CCWWGG 1 cut(s) 777
EcoT38I GRGCYC 1 cut(s) 167
EgeI GGCGCC 2 cut(s) 156, 177
EheI GGCGCC 2 cut(s) 156, 177
ErhI CCWWGG 1 cut(s) 777
FaeI CATG 1 cut(s) 403
FaiI YATR 4 cut(s) 293, 377, 401, 439
FaqI GGGAC 1 cut(s) 97
FatI CATG 1 cut(s) 399
FauI CCCGC 1 cut(s) 266
FbaI TGATCA 1 cut(s) 562
Fnu4HI GCNGC 6 cut(s) 12, 61, 153, 197, 206, 506
FokI GGATG 3 cut(s) 10, 523, 559
FriOI GRGCYC 1 cut(s) 167
Fsp4HI GCNGC 6 cut(s) 12, 61, 153, 197, 206, 506
FspBI CTAG 1 cut(s) 485
GlaI GCGC 3 cut(s) 156, 177, 751
GluI GCNGC 6 cut(s) 12, 61, 153, 197, 206, 506
HaeII RGCGCY 2 cut(s) 158, 179
HaeIII GGCC 1 cut(s) 354
HapII CCGG 2 cut(s) 237, 371
HhaI GCGC 3 cut(s) 157, 178, 752
Hin1I GRCGYC 3 cut(s) 155, 176, 613
Hin1II CATG 1 cut(s) 403
Hin6I GCGC 3 cut(s) 155, 176, 750
HinP1I GCGC 3 cut(s) 155, 176, 750
HincII GTYRAC 1 cut(s) 640
HindII GTYRAC 1 cut(s) 640
HinfI GANTC 6 cut(s) 117, 225, 416, 448, 577, 600
HpaII CCGG 2 cut(s) 237, 371
HphI GGTGA 2 cut(s) 120, 425
Hpy166II GTNNAC 2 cut(s) 527, 640
Hpy188I TCNGA 4 cut(s) 105, 169, 224, 230
Hpy188III TCNNGA 5 cut(s) 114, 485, 581, 668, 698
Hpy8I GTNNAC 2 cut(s) 527, 640
Hpy99I CGWCG 2 cut(s) 100, 224
HpyAV CCTTC 1 cut(s) 299
HpyCH4III ACNGT 2 cut(s) 37, 145
HpyCH4IV ACGT 3 cut(s) 6, 613, 692
HpyF10VI GCNNNNNNNGC 7 cut(s) 60, 66, 202, 205, 211, 360, 514
HpySE526I ACGT 3 cut(s) 6, 613, 692
Hsp92I GRCGYC 3 cut(s) 155, 176, 613
Hsp92II CATG 1 cut(s) 403
HspAI GCGC 3 cut(s) 155, 176, 750
KasI GGCGCC 2 cut(s) 154, 175
Ksp22I TGATCA 1 cut(s) 562
KspI CCGCGG 1 cut(s) 153
Kzo9I GATC 6 cut(s) 89, 425, 481, 487, 562, 631
LpnPI CCDG 7 cut(s) 171, 192, 250, 384, 606, 633, 648
Lsp1109I GCAGC 3 cut(s) 47, 217, 492
LweI GCATC 1 cut(s) 277
MaeI CTAG 1 cut(s) 485
MaeII ACGT 3 cut(s) 6, 613, 692
MalI GATC 6 cut(s) 91, 427, 483, 489, 564, 633
MboI GATC 6 cut(s) 89, 425, 481, 487, 562, 631
MboII GAAGA 3 cut(s) 253, 482, 641
MfeI CAATTG 1 cut(s) 49
MflI RGATCY 2 cut(s) 487, 631
MhlI GDGCHC 1 cut(s) 167
MluCI AATT 2 cut(s) 49, 685
Mly113I GGCGCC 2 cut(s) 155, 176
MlyI GAGTC 3 cut(s) 219, 425, 586
MmeI TCCRAC 3 cut(s) 202, 325, 403
MnlI CCTC 6 cut(s) 148, 527, 543, 600, 627, 727
MseI TTAA 4 cut(s) 570, 596, 762, 793
MspA1I CMGCKG 2 cut(s) 60, 152
MspI CCGG 2 cut(s) 237, 371
MspR9I CCNGG 3 cut(s) 237, 238, 621
MunI CAATTG 1 cut(s) 49
MvaI CCWGG 1 cut(s) 621
MvnI CGCG 1 cut(s) 152
MwoI GCNNNNNNNGC 7 cut(s) 60, 66, 202, 205, 211, 360, 514
NarI GGCGCC 2 cut(s) 155, 176
NciI CCSGG 2 cut(s) 237, 238
NdeII GATC 6 cut(s) 89, 425, 481, 487, 562, 631
NlaIII CATG 1 cut(s) 403
NlaIV GGNNCC 3 cut(s) 156, 177, 633
PaeR7I CTCGAG 1 cut(s) 731
PceI AGGCCT 1 cut(s) 354
PfeI GAWTC 3 cut(s) 117, 448, 600
PflMI CCANNNNNTGG 2 cut(s) 268, 524
PkrI GCNGC 6 cut(s) 13, 62, 154, 198, 207, 507
PleI GAGTC 3 cut(s) 219, 424, 585
PluTI GGCGCC 2 cut(s) 158, 179
PpsI GAGTC 3 cut(s) 219, 424, 585
Psp124BI GAGCTC 1 cut(s) 167
Psp1406I AACGTT 1 cut(s) 6
Psp6I CCWGG 1 cut(s) 619
PspGI CCWGG 1 cut(s) 619
PspN4I GGNNCC 3 cut(s) 156, 177, 633
PspXI VCTCGAGB 1 cut(s) 731
PsuI RGATCY 2 cut(s) 487, 631
RsaI GTAC 2 cut(s) 248, 465
RsaNI GTAC 2 cut(s) 247, 464
SacI GAGCTC 1 cut(s) 167
SacII CCGCGG 1 cut(s) 153
SaqAI TTAA 4 cut(s) 570, 596, 762, 793
SatI GCNGC 6 cut(s) 12, 61, 153, 197, 206, 506
Sau3AI GATC 6 cut(s) 89, 425, 481, 487, 562, 631
ScaI AGTACT 1 cut(s) 465
SchI GAGTC 3 cut(s) 219, 425, 586
ScrFI CCNGG 3 cut(s) 237, 238, 621
SduI GDGCHC 1 cut(s) 167
SetI ASST 9 cut(s) 9, 167, 298, 304, 316, 507, 526, 616, 695
SfaNI GCATC 1 cut(s) 277
SfoI GGCGCC 2 cut(s) 156, 177
Sfr274I CTCGAG 1 cut(s) 731
Sfr303I CCGCGG 1 cut(s) 153
SgrBI CCGCGG 1 cut(s) 153
SlaI CTCGAG 1 cut(s) 731
SmaI CCCGGG 1 cut(s) 238
SmlI CTYRAG 1 cut(s) 731
SmoI CTYRAG 1 cut(s) 731
Sse9I AATT 2 cut(s) 49, 685
SseBI AGGCCT 1 cut(s) 354
SspDI GGCGCC 2 cut(s) 154, 175
SspMI CTAG 1 cut(s) 485
SstI GAGCTC 1 cut(s) 167
StuI AGGCCT 1 cut(s) 354
StyD4I CCNGG 3 cut(s) 235, 236, 619
StyI CCWWGG 1 cut(s) 777
TaaI ACNGT 2 cut(s) 37, 145
TaiI ACGT 3 cut(s) 9, 616, 695
TaqI TCGA 5 cut(s) 88, 183, 428, 580, 732
TasI AATT 2 cut(s) 49, 685
TatI WGTACW 2 cut(s) 246, 463
TauI GCSGC 2 cut(s) 155, 199
TfiI GAWTC 3 cut(s) 117, 448, 600
Tru1I TTAA 4 cut(s) 570, 596, 762, 793
Tru9I TTAA 4 cut(s) 570, 596, 762, 793
TscAI CASTG 2 cut(s) 165, 328
TseI GCWGC 4 cut(s) 11, 60, 205, 505
TspDTI ATGAA 3 cut(s) 17, 416, 426
TspGWI ACGGA 2 cut(s) 84, 546
TspMI CCCGGG 1 cut(s) 236
TspRI CASTG 2 cut(s) 165, 328
Van91I CCANNNNNTGG 2 cut(s) 268, 524
XapI RAATTY 1 cut(s) 685
XbaI TCTAGA 1 cut(s) 484
XhoI CTCGAG 1 cut(s) 731
XmaI CCCGGG 1 cut(s) 236
XspI CTAG 1 cut(s) 485
ZraI GACGTC 1 cut(s) 614
ZrmI AGTACT 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.