Rh7AG077500

Belongs to the PTH family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0007236
Physical Location & Seq
Forward (+)
5541056 .. 5544159
3104 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG077500.1

Sequence Viewer

Length: 720 bp
ATGTCCATACTTCTCCAACACTCAAACAATTATTTGCTTCATACAAATCACATCCAATCAAAAATGAGCGTCGCTGCTTCTATATCCTCCATCGCCACCGTTCGTTTCCCCAATTGCCGCCGTCACTGCTTTCGCGCCTCACCATCTCTCTCGCTTCGGATAATGGCCCAATTCTCATCCTCCGCCGCGTCTTCTCCGCCTTCCAGCTCCGAAGCGGCCGCCGTGGAACCCAAGAAACCGCCCAAGCTGCCGTGGCTCATTGTCGGACTCGGAAACCCCGGCAAGAAGTACAGCAGCACCCGCCACAATGTGGGTTTCGAGATGGTGGATGCTATAGCCGAAGCTGAAGGGATAGCTTTGAGCAGTGTTTCTTTCAAAGCCCTTGTTGGGAAAGGATTTATTGGGGATGCCCCGGTTATATTTGCCAAACCGCAAACTTACATGAATAAAAGCGGTGAGTCTGTTGGAGCCATCGTCTCATATTACAAAATTCCATTGCAGCAAGTACTTGTGATCTTTGACGATCTGGATCTGCCATTCACAAAATTGCGGCTGTTGCCAAAGGGTGGACATGGAGGACAAAATGGGATGAGAAGTATAATTGATCATTTTAAAGGAAGCCGTGACTTTCCACGTTTGAGAATCGGTAATTTACCTAGAGCACCGGAAAGTCTTTCCAAAATACGTATATGCAAATTTTTATTGAAATTGAATTGGTGA

Protein Analysis

239

Amino Acids

26.12

Weight (kDa)

10.08

Isoelectric Point (pI)

49.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 86 - 216 9e-52 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 310, 566
AccII CGCG 2 cut(s) 135, 188
AclWI GGATC 1 cut(s) 537
AcoI YGGCCR 1 cut(s) 216
AcsI RAATTY 2 cut(s) 489, 695
AcuI CTGAAG 1 cut(s) 366
AdeI CACNNNGTG 1 cut(s) 310
AfaI GTAC 2 cut(s) 290, 507
AfiI CCNNNNNNNGG 3 cut(s) 310, 387, 566
AgsI TTSAA 3 cut(s) 376, 706, 712
AluBI AGCT 4 cut(s) 207, 247, 344, 356
AluI AGCT 4 cut(s) 207, 247, 344, 356
Alw21I GWGCWC 1 cut(s) 664
Alw26I GTCTC 1 cut(s) 481
AlwI GGATC 1 cut(s) 537
AoxI GGCC 2 cut(s) 165, 216
ApeKI GCWGC 4 cut(s) 74, 247, 294, 499
ApoI RAATTY 2 cut(s) 489, 695
AspLEI GCGC 1 cut(s) 137
AspS9I GGNCC 1 cut(s) 166
AsuC2I CCSGG 2 cut(s) 279, 413
AsuHPI GGTGA 2 cut(s) 132, 467
BbsI GAAGAC 1 cut(s) 183
Bbv12I GWGCWC 1 cut(s) 664
BbvI GCAGC 4 cut(s) 61, 234, 306, 511
BccI CCATC 4 cut(s) 98, 151, 316, 479
BceAI ACGGC 4 cut(s) 105, 206, 235, 606
BclI TGATCA 1 cut(s) 604
BcnI CCSGG 2 cut(s) 279, 413
BcoDI GTCTC 1 cut(s) 481
BfaI CTAG 1 cut(s) 657
BfmI CTRYAG 1 cut(s) 333
BisI GCNGC 9 cut(s) 75, 118, 186, 216, 219, 248, 295, 500, 551
BlsI GCNGC 9 cut(s) 76, 119, 187, 217, 220, 249, 296, 501, 552
BmcAI AGTACT 1 cut(s) 507
Bme1390I CCNGG 2 cut(s) 279, 413
BmgT120I GGNCC 1 cut(s) 166
BmiI GGNNCC 2 cut(s) 228, 469
BmrFI CCNGG 2 cut(s) 279, 413
BmsI GCATC 2 cut(s) 319, 397
BpiI GAAGAC 1 cut(s) 183
BpuMI CCSGG 2 cut(s) 279, 413
BsaAI YACGTR 1 cut(s) 686
BsaBI GATNNNNATC 1 cut(s) 528
BsaJI CCNNGG 4 cut(s) 222, 251, 277, 411
BsaWI WCCGGW 1 cut(s) 664
BsaXI ACNNNNNCTCC 2 cut(s) 459, 489
Bsc4I CCNNNNNNNGG 3 cut(s) 310, 387, 566
Bse3DI GCAATG 1 cut(s) 494
Bse8I GATNNNNATC 1 cut(s) 528
BseDI CCNNGG 4 cut(s) 222, 251, 277, 411
BseGI GGATG 5 cut(s) 51, 176, 334, 412, 594
BseJI GATNNNNATC 1 cut(s) 528
BseLI CCNNNNNNNGG 3 cut(s) 310, 387, 566
BseMI GCAATG 1 cut(s) 494
BseX3I CGGCCG 1 cut(s) 216
BseXI GCAGC 4 cut(s) 61, 234, 306, 511
Bsh1236I CGCG 2 cut(s) 135, 188
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 2 cut(s) 167, 218
BsiEI CGRYCG 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 664
BsiSI CCGG 3 cut(s) 279, 413, 665
BslI CCNNNNNNNGG 3 cut(s) 310, 387, 566
BsmAI GTCTC 1 cut(s) 481
BsmBI CGTCTC 1 cut(s) 481
BsnI GGCC 2 cut(s) 167, 218
Bsp1286I GDGCHC 1 cut(s) 664
Bsp143I GATC 4 cut(s) 513, 523, 529, 604
BspANI GGCC 2 cut(s) 167, 218
BspFNI CGCG 2 cut(s) 135, 188
BspLI GGNNCC 2 cut(s) 228, 469
BspPI GGATC 1 cut(s) 537
BsrDI GCAATG 1 cut(s) 494
BssECI CCNNGG 4 cut(s) 222, 251, 277, 411
BssMI GATC 4 cut(s) 513, 523, 529, 604
Bst4CI ACNGT 1 cut(s) 100
BstBAI YACGTR 1 cut(s) 686
BstDSI CCRYGG 2 cut(s) 222, 251
BstF5I GGATG 5 cut(s) 51, 176, 334, 412, 594
BstFNI CGCG 2 cut(s) 135, 188
BstHHI GCGC 1 cut(s) 137
BstKTI GATC 4 cut(s) 516, 526, 532, 607
BstMAI GTCTC 1 cut(s) 481
BstMBI GATC 4 cut(s) 513, 523, 529, 604
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 5 cut(s) 126, 247, 253, 300, 556
BstSCI CCNGG 2 cut(s) 277, 411
BstSFI CTRYAG 1 cut(s) 333
BstSNI TACGTA 1 cut(s) 686
BstUI CGCG 2 cut(s) 135, 188
BstV1I GCAGC 4 cut(s) 61, 234, 306, 511
BstV2I GAAGAC 1 cut(s) 183
BstX2I RGATCY 1 cut(s) 529
BstYI RGATCY 1 cut(s) 529
BstZI CGGCCG 1 cut(s) 216
BsuRI GGCC 2 cut(s) 167, 218
BtgI CCRYGG 2 cut(s) 222, 251
BtgZI GCGATG 1 cut(s) 76
BtsCI GGATG 5 cut(s) 51, 176, 334, 412, 594
BtsI GCAGTG 2 cut(s) 124, 370
BtsIMutI CAGTG 2 cut(s) 124, 370
CciNI GCGGCCGC 1 cut(s) 216
CfoI GCGC 1 cut(s) 137
Cfr13I GGNCC 1 cut(s) 166
CseI GACGC 2 cut(s) 58, 177
Csp6I GTAC 2 cut(s) 289, 506
CviAII CATG 2 cut(s) 442, 572
CviQI GTAC 2 cut(s) 289, 506
DpnI GATC 4 cut(s) 515, 525, 531, 606
DpnII GATC 4 cut(s) 513, 523, 529, 604
DraI TTTAAA 1 cut(s) 613
DraIII CACNNNGTG 1 cut(s) 310
EaeI YGGCCR 1 cut(s) 216
EagI CGGCCG 1 cut(s) 216
EciI GGCGGA 2 cut(s) 172, 186
EclXI CGGCCG 1 cut(s) 216
Eco105I TACGTA 1 cut(s) 686
Eco52I CGGCCG 1 cut(s) 216
Eco57I CTGAAG 1 cut(s) 366
Esp3I CGTCTC 1 cut(s) 481
FaeI CATG 2 cut(s) 445, 575
FatI CATG 2 cut(s) 441, 571
FauI CCCGC 1 cut(s) 308
FbaI TGATCA 1 cut(s) 604
Fnu4HI GCNGC 9 cut(s) 75, 118, 186, 216, 219, 248, 295, 500, 551
FokI GGATG 5 cut(s) 38, 163, 341, 419, 601
Fsp4HI GCNGC 9 cut(s) 75, 118, 186, 216, 219, 248, 295, 500, 551
FspBI CTAG 1 cut(s) 657
GlaI GCGC 1 cut(s) 136
GluI GCNGC 9 cut(s) 75, 118, 186, 216, 219, 248, 295, 500, 551
HaeIII GGCC 2 cut(s) 167, 218
HapII CCGG 3 cut(s) 279, 413, 665
HgaI GACGC 2 cut(s) 58, 177
HhaI GCGC 1 cut(s) 137
Hin1II CATG 2 cut(s) 445, 575
Hin6I GCGC 1 cut(s) 135
HinP1I GCGC 1 cut(s) 135
HinfI GANTC 3 cut(s) 267, 458, 642
HpaII CCGG 3 cut(s) 279, 413, 665
HphI GGTGA 2 cut(s) 132, 467
Hpy166II GTNNAC 1 cut(s) 569
Hpy188I TCNGA 4 cut(s) 159, 211, 266, 272
Hpy188III TCNNGA 2 cut(s) 319, 527
Hpy8I GTNNAC 1 cut(s) 569
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 2 cut(s) 210, 341
HpyCH4III ACNGT 1 cut(s) 100
HpyCH4IV ACGT 2 cut(s) 634, 685
HpyCH4V TGCA 2 cut(s) 499, 693
HpyF10VI GCNNNNNNNGC 5 cut(s) 126, 247, 253, 300, 556
HpySE526I ACGT 2 cut(s) 634, 685
Hsp92II CATG 2 cut(s) 445, 575
HspAI GCGC 1 cut(s) 135
Ksp22I TGATCA 1 cut(s) 604
Kzo9I GATC 4 cut(s) 513, 523, 529, 604
LmnI GCTCC 2 cut(s) 212, 467
LpnPI CCDG 5 cut(s) 217, 292, 426, 512, 678
Lsp1109I GCAGC 4 cut(s) 61, 234, 306, 511
LweI GCATC 2 cut(s) 319, 397
MaeI CTAG 1 cut(s) 657
MaeII ACGT 2 cut(s) 634, 685
MaeIII GTNAC 2 cut(s) 122, 623
MalI GATC 4 cut(s) 515, 525, 531, 606
MboI GATC 4 cut(s) 513, 523, 529, 604
MboII GAAGA 1 cut(s) 183
MfeI CAATTG 1 cut(s) 112
MflI RGATCY 1 cut(s) 529
MhlI GDGCHC 1 cut(s) 664
MlyI GAGTC 2 cut(s) 261, 467
MmeI TCCRAC 3 cut(s) 40, 244, 445
MnlI CCTC 4 cut(s) 97, 148, 190, 569
MseI TTAA 1 cut(s) 612
MspI CCGG 3 cut(s) 279, 413, 665
MspR9I CCNGG 2 cut(s) 279, 413
MunI CAATTG 1 cut(s) 112
MvnI CGCG 2 cut(s) 135, 188
MwoI GCNNNNNNNGC 5 cut(s) 126, 247, 253, 300, 556
NciI CCSGG 2 cut(s) 279, 413
NdeII GATC 4 cut(s) 513, 523, 529, 604
NlaIII CATG 2 cut(s) 445, 575
NlaIV GGNNCC 2 cut(s) 228, 469
NmuCI GTSAC 2 cut(s) 122, 623
NotI GCGGCCGC 1 cut(s) 216
PfeI GAWTC 1 cut(s) 642
PflMI CCANNNNNTGG 2 cut(s) 310, 566
PkrI GCNGC 9 cut(s) 76, 119, 187, 217, 220, 249, 296, 501, 552
PleI GAGTC 2 cut(s) 261, 466
PpsI GAGTC 2 cut(s) 261, 466
Ppu21I YACGTR 1 cut(s) 686
PspN4I GGNNCC 2 cut(s) 228, 469
PspPI GGNCC 1 cut(s) 166
PsuI RGATCY 1 cut(s) 529
RsaI GTAC 2 cut(s) 290, 507
RsaNI GTAC 2 cut(s) 289, 506
SaqAI TTAA 1 cut(s) 612
SatI GCNGC 9 cut(s) 75, 118, 186, 216, 219, 248, 295, 500, 551
Sau3AI GATC 4 cut(s) 513, 523, 529, 604
Sau96I GGNCC 1 cut(s) 166
ScaI AGTACT 1 cut(s) 507
SchI GAGTC 2 cut(s) 261, 467
ScrFI CCNGG 2 cut(s) 279, 413
SduI GDGCHC 1 cut(s) 664
SetI ASST 7 cut(s) 209, 249, 346, 358, 637, 658, 688
SfaNI GCATC 2 cut(s) 319, 397
SfcI CTRYAG 1 cut(s) 333
SnaBI TACGTA 1 cut(s) 686
SspMI CTAG 1 cut(s) 657
StyD4I CCNGG 2 cut(s) 277, 411
TaaI ACNGT 1 cut(s) 100
TaiI ACGT 2 cut(s) 637, 688
TaqI TCGA 1 cut(s) 318
TatI WGTACW 2 cut(s) 288, 505
TauI GCSGC 5 cut(s) 120, 188, 218, 221, 553
TfiI GAWTC 1 cut(s) 642
Tru1I TTAA 1 cut(s) 612
Tru9I TTAA 1 cut(s) 612
TscAI CASTG 2 cut(s) 131, 370
TseFI GTSAC 2 cut(s) 122, 623
TseI GCWGC 4 cut(s) 74, 247, 294, 499
Tsp45I GTSAC 2 cut(s) 122, 623
TspDTI ATGAA 2 cut(s) 29, 458
TspRI CASTG 2 cut(s) 131, 370
Van91I CCANNNNNTGG 2 cut(s) 310, 566
XapI RAATTY 2 cut(s) 489, 695
XspI CTAG 1 cut(s) 657
ZrmI AGTACT 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.