AT1G20310

At1g76070-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008592
Physical Location & Seq
Forward (+)
7032070 .. 7033329
1260 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G20310.1

Sequence Viewer

Length: 723 bp
ATGGAAAAAGCTTCAAACAGCAACAAGTCCACGAAATTCTCGAAAATGCTTCAAAGAGCGATGTCAATTGGTCACAGTGCGGCGCCGTTTAGTCCACGGAGAGATTTTCATCAGCACCGTACGACTTCGACGGCGAACAGAGGAATCTTCTTCTCCAGTCCGTTGGTTCCTACGGCTGCTAGAGTTAGGAGAAATACAAAATACGAAGCCGTTTTTGCCGAACCCACTTCGCCTAAAGTCTCCTGCATCGGTCAGGTCAAACTCGCTAGACCTAAATGTCCGGAGAAGAAGAACAAAGCACCTAAGAATCTCAAAACGGCATCGTCTTTATCGTCTTGTGTTATTAAAGAAGAAGACAATGGAAGCTTTTCGAAACTAAAGCGTATCTTCTCAATGAGAAGTTATCCGTCGAGAAAATCCAACTCCACTGCCTTCGCAGCCGCCGCAGCGAGAGAACATCCAATCGCGGAGGTTGATGCGGTTACGGCAGCGCCTTCATTGGGTGCAATGAAGAAATTCGCGAGCAGTAGAGAAGCTCTCGGTGGATTTGATTGGACGGTCCAGATGAAACGTGAGAAAGAAGATGTAATGATTCCATGCTCTGTGGGTATACCTCTAACTCAGCTAGAGGATTTGAGTTTGTGTCCGAAGCCAAAGAGTGAAGTTAATTTGTGGAAGAGAAGAACCATGGATCGACCAAAACCACTTCAAGTAATTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

240

Amino Acids

26.59

Weight (kDa)

10.25

Isoelectric Point (pI)

50.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 276
AccB1I GGYRCC 1 cut(s) 82
AccI GTMKAC 1 cut(s) 610
AccII CGCG 2 cut(s) 467, 521
AccIII TCCGGA 1 cut(s) 280
AciI CCGC 5 cut(s) 80, 441, 444, 467, 479
AclWI GGATC 1 cut(s) 699
AcsI RAATTY 2 cut(s) 35, 515
AcyI GRCGYC 1 cut(s) 83
AfaI GTAC 1 cut(s) 121
AfiI CCNNNNNNNGG 1 cut(s) 500
AgsI TTSAA 3 cut(s) 15, 53, 710
AluBI AGCT 4 cut(s) 11, 366, 536, 625
AluI AGCT 4 cut(s) 11, 366, 536, 625
Alw26I GTCTC 1 cut(s) 244
AlwI GGATC 1 cut(s) 699
Aor13HI TCCGGA 1 cut(s) 280
ApeKI GCWGC 4 cut(s) 176, 437, 446, 488
ApoI RAATTY 2 cut(s) 35, 515
Asp700I GAANNNNTTC 2 cut(s) 367, 515
AspLEI GCGC 2 cut(s) 85, 493
AspS9I GGNCC 1 cut(s) 559
AsuII TTCGAA 1 cut(s) 371
AvaII GGWCC 1 cut(s) 559
BanI GGYRCC 1 cut(s) 82
BbsI GAAGAC 1 cut(s) 360
BbvI GCAGC 4 cut(s) 163, 449, 458, 500
BceAI ACGGC 6 cut(s) 70, 147, 189, 194, 333, 501
BcoDI GTCTC 1 cut(s) 244
BfaI CTAG 3 cut(s) 180, 267, 626
BfoI RGCGCY 2 cut(s) 86, 494
BisI GCNGC 7 cut(s) 81, 177, 438, 441, 444, 447, 489
BlsI GCNGC 7 cut(s) 82, 178, 439, 442, 445, 448, 490
Bme18I GGWCC 1 cut(s) 559
BmgT120I GGNCC 1 cut(s) 559
BmiI GGNNCC 2 cut(s) 84, 168
BmsI GCATC 3 cut(s) 255, 329, 466
BpiI GAAGAC 1 cut(s) 360
BplI GAGNNNNNCTC 2 cut(s) 522, 554
BpmI CTGGAG 1 cut(s) 139
Bpu14I TTCGAA 1 cut(s) 371
BsaBI GATNNNNATC 1 cut(s) 108
BsaHI GRCGYC 1 cut(s) 83
BsaJI CCNNGG 2 cut(s) 95, 687
BsaWI WCCGGW 1 cut(s) 280
Bsc4I CCNNNNNNNGG 1 cut(s) 500
Bse1I ACTGG 1 cut(s) 156
Bse3DI GCAATG 1 cut(s) 513
Bse8I GATNNNNATC 1 cut(s) 108
BseAI TCCGGA 1 cut(s) 280
BseDI CCNNGG 2 cut(s) 95, 687
BseGI GGATG 1 cut(s) 457
BseJI GATNNNNATC 1 cut(s) 108
BseLI CCNNNNNNNGG 1 cut(s) 500
BseMI GCAATG 1 cut(s) 513
BseMII CTCAG 1 cut(s) 635
BseNI ACTGG 1 cut(s) 156
BseXI GCAGC 4 cut(s) 163, 449, 458, 500
Bsh1236I CGCG 2 cut(s) 467, 521
BshNI GGYRCC 1 cut(s) 82
BsiSI CCGG 1 cut(s) 281
BsiWI CGTACG 1 cut(s) 119
BslI CCNNNNNNNGG 1 cut(s) 500
BsmAI GTCTC 1 cut(s) 244
Bsp119I TTCGAA 1 cut(s) 371
Bsp13I TCCGGA 1 cut(s) 280
Bsp143I GATC 1 cut(s) 691
Bsp19I CCATGG 1 cut(s) 687
Bsp68I TCGCGA 1 cut(s) 521
BspACI CCGC 5 cut(s) 80, 441, 444, 467, 479
BspCNI CTCAG 1 cut(s) 634
BspEI TCCGGA 1 cut(s) 280
BspFNI CGCG 2 cut(s) 467, 521
BspLI GGNNCC 2 cut(s) 84, 168
BspPI GGATC 1 cut(s) 699
BspT104I TTCGAA 1 cut(s) 371
BspT107I GGYRCC 1 cut(s) 82
BsrDI GCAATG 1 cut(s) 513
BsrI ACTGG 1 cut(s) 156
BssECI CCNNGG 2 cut(s) 95, 687
BssMI GATC 1 cut(s) 691
BssNAI GTATAC 1 cut(s) 611
BssNI GRCGYC 1 cut(s) 83
BssT1I CCWWGG 1 cut(s) 687
Bst1107I GTATAC 1 cut(s) 611
Bst4CI ACNGT 3 cut(s) 77, 119, 559
Bst6I CTCTTC 1 cut(s) 671
BstACI GRCGYC 1 cut(s) 83
BstBI TTCGAA 1 cut(s) 371
BstC8I GCNNGC 1 cut(s) 523
BstDEI CTNAG 2 cut(s) 303, 621
BstDSI CCRYGG 2 cut(s) 95, 687
BstF5I GGATG 1 cut(s) 457
BstFNI CGCG 2 cut(s) 467, 521
BstH2I RGCGCY 2 cut(s) 86, 494
BstHHI GCGC 2 cut(s) 85, 493
BstKTI GATC 1 cut(s) 694
BstMAI GTCTC 1 cut(s) 244
BstMBI GATC 1 cut(s) 691
BstMWI GCNNNNNNNGC 5 cut(s) 215, 437, 443, 446, 485
BstUI CGCG 2 cut(s) 467, 521
BstV1I GCAGC 4 cut(s) 163, 449, 458, 500
BstV2I GAAGAC 1 cut(s) 360
BstXI CCANNNNNNTGG 1 cut(s) 163
BstZ17I GTATAC 1 cut(s) 611
BtgI CCRYGG 2 cut(s) 95, 687
BtgZI GCGATG 1 cut(s) 74
BtsCI GGATG 1 cut(s) 457
BtsI GCAGTG 1 cut(s) 426
BtsIMutI CAGTG 2 cut(s) 82, 426
BtuMI TCGCGA 1 cut(s) 521
Cac8I GCNNGC 1 cut(s) 523
CfoI GCGC 2 cut(s) 85, 493
Cfr13I GGNCC 1 cut(s) 559
Csp6I GTAC 1 cut(s) 120
CviAII CATG 2 cut(s) 597, 688
CviJI RGCY 8 cut(s) 11, 176, 209, 366, 440, 536, 625, 652
CviKI_1 RGCY 8 cut(s) 11, 176, 209, 366, 440, 536, 625, 652
CviQI GTAC 1 cut(s) 120
DdeI CTNAG 2 cut(s) 303, 621
DinI GGCGCC 1 cut(s) 84
DpnI GATC 1 cut(s) 693
DpnII GATC 1 cut(s) 691
DrdI GACNNNNNNGTC 1 cut(s) 276
DseDI GACNNNNNNGTC 1 cut(s) 276
Eam1104I CTCTTC 1 cut(s) 671
EarI CTCTTC 1 cut(s) 671
Eco130I CCWWGG 1 cut(s) 687
Eco47I GGWCC 1 cut(s) 559
EcoT14I CCWWGG 1 cut(s) 687
EgeI GGCGCC 1 cut(s) 84
EheI GGCGCC 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 687
FaeI CATG 2 cut(s) 600, 691
FaiI YATR 3 cut(s) 598, 611, 689
FalI AAGNNNNNCTT 2 cut(s) 371, 403
FatI CATG 2 cut(s) 596, 687
FblI GTMKAC 1 cut(s) 610
Fnu4HI GCNGC 7 cut(s) 81, 177, 438, 441, 444, 447, 489
FokI GGATG 1 cut(s) 444
Fsp4HI GCNGC 7 cut(s) 81, 177, 438, 441, 444, 447, 489
FspBI CTAG 3 cut(s) 180, 267, 626
GlaI GCGC 2 cut(s) 84, 492
GluI GCNGC 7 cut(s) 81, 177, 438, 441, 444, 447, 489
GsuI CTGGAG 1 cut(s) 139
HaeII RGCGCY 2 cut(s) 86, 494
HapII CCGG 1 cut(s) 281
HhaI GCGC 2 cut(s) 85, 493
Hin1I GRCGYC 1 cut(s) 83
Hin1II CATG 2 cut(s) 600, 691
Hin6I GCGC 2 cut(s) 83, 491
HinP1I GCGC 2 cut(s) 83, 491
HindIII AAGCTT 2 cut(s) 9, 364
HinfI GANTC 3 cut(s) 144, 307, 592
HpaII CCGG 1 cut(s) 281
Hpy166II GTNNAC 3 cut(s) 30, 95, 611
Hpy188I TCNGA 1 cut(s) 648
Hpy188III TCNNGA 5 cut(s) 40, 281, 411, 520, 562
Hpy8I GTNNAC 3 cut(s) 30, 95, 611
Hpy99I CGWCG 2 cut(s) 133, 412
HpyAV CCTTC 2 cut(s) 442, 504
HpyCH4III ACNGT 3 cut(s) 77, 119, 559
HpyCH4IV ACGT 1 cut(s) 571
HpyCH4V TGCA 2 cut(s) 246, 506
HpyF10VI GCNNNNNNNGC 5 cut(s) 215, 437, 443, 446, 485
HpyF3I CTNAG 2 cut(s) 303, 621
HpySE526I ACGT 1 cut(s) 571
Hsp92I GRCGYC 1 cut(s) 83
Hsp92II CATG 2 cut(s) 600, 691
HspAI GCGC 2 cut(s) 83, 491
KasI GGCGCC 1 cut(s) 82
Kpn2I TCCGGA 1 cut(s) 280
Kzo9I GATC 1 cut(s) 691
LpnPI CCDG 5 cut(s) 169, 239, 256, 294, 575
Lsp1109I GCAGC 4 cut(s) 163, 449, 458, 500
LweI GCATC 3 cut(s) 255, 329, 466
MaeI CTAG 3 cut(s) 180, 267, 626
MaeII ACGT 1 cut(s) 571
MaeIII GTNAC 2 cut(s) 71, 481
MalI GATC 1 cut(s) 693
MboI GATC 1 cut(s) 691
MfeI CAATTG 1 cut(s) 66
MluCI AATT 5 cut(s) 35, 66, 515, 667, 714
Mly113I GGCGCC 1 cut(s) 83
MmeI TCCRAC 1 cut(s) 444
MnlI CCTC 4 cut(s) 134, 463, 622, 624
MroI TCCGGA 1 cut(s) 280
MroXI GAANNNNTTC 2 cut(s) 367, 515
MseI TTAA 3 cut(s) 345, 666, 717
MspI CCGG 1 cut(s) 281
MunI CAATTG 1 cut(s) 66
MvnI CGCG 2 cut(s) 467, 521
MwoI GCNNNNNNNGC 5 cut(s) 215, 437, 443, 446, 485
NarI GGCGCC 1 cut(s) 83
NcoI CCATGG 1 cut(s) 687
NdeII GATC 1 cut(s) 691
NlaIII CATG 2 cut(s) 600, 691
NlaIV GGNNCC 2 cut(s) 84, 168
NmuCI GTSAC 1 cut(s) 71
NruI TCGCGA 1 cut(s) 521
NspV TTCGAA 1 cut(s) 371
PcsI WCGNNNNNNNCGW 2 cut(s) 38, 329
PdmI GAANNNNTTC 2 cut(s) 367, 515
PfeI GAWTC 3 cut(s) 144, 307, 592
Pfl23II CGTACG 1 cut(s) 119
PkrI GCNGC 7 cut(s) 82, 178, 439, 442, 445, 448, 490
PluTI GGCGCC 1 cut(s) 86
PspLI CGTACG 1 cut(s) 119
PspN4I GGNNCC 2 cut(s) 84, 168
PspPI GGNCC 1 cut(s) 559
RruI TCGCGA 1 cut(s) 521
RsaI GTAC 1 cut(s) 121
RsaNI GTAC 1 cut(s) 120
SaqAI TTAA 3 cut(s) 345, 666, 717
SatI GCNGC 7 cut(s) 81, 177, 438, 441, 444, 447, 489
Sau3AI GATC 1 cut(s) 691
Sau96I GGNCC 1 cut(s) 559
SfaNI GCATC 3 cut(s) 255, 329, 466
SfoI GGCGCC 1 cut(s) 84
SfuI TTCGAA 1 cut(s) 371
SinI GGWCC 1 cut(s) 559
Sse9I AATT 5 cut(s) 35, 66, 515, 667, 714
SsiI CCGC 5 cut(s) 80, 441, 444, 467, 479
SspDI GGCGCC 1 cut(s) 82
SspMI CTAG 3 cut(s) 180, 267, 626
StyI CCWWGG 1 cut(s) 687
TaaI ACNGT 3 cut(s) 77, 119, 559
TaiI ACGT 1 cut(s) 574
TaqI TCGA 5 cut(s) 41, 128, 371, 410, 694
TaqII GACCGA 1 cut(s) 239
TasI AATT 5 cut(s) 35, 66, 515, 667, 714
TauI GCSGC 3 cut(s) 83, 443, 446
TfiI GAWTC 3 cut(s) 144, 307, 592
Tru1I TTAA 3 cut(s) 345, 666, 717
Tru9I TTAA 3 cut(s) 345, 666, 717
TscAI CASTG 2 cut(s) 82, 433
TseFI GTSAC 1 cut(s) 71
TseI GCWGC 4 cut(s) 176, 437, 446, 488
Tsp45I GTSAC 1 cut(s) 71
TspDTI ATGAA 4 cut(s) 98, 486, 524, 581
TspGWI ACGGA 3 cut(s) 112, 150, 396
TspRI CASTG 2 cut(s) 82, 433
VpaK11BI GGWCC 1 cut(s) 559
XapI RAATTY 2 cut(s) 35, 515
XmiI GTMKAC 1 cut(s) 610
XmnI GAANNNNTTC 2 cut(s) 367, 515
XspI CTAG 3 cut(s) 180, 267, 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.