Rh6CG450000

At1g76070-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0008592
Physical Location & Seq
Forward (+)
62734161 .. 62735680
1520 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG450000.1

Sequence Viewer

Length: 789 bp
ATGGAACTTCAAAAACAAGGTAAATCCAGAAGCAAGATATTGAAATTCCTACCCAAAGCTGCCTCTGCGGTTTACTTTCATGGCGCTGCTTACAGCCCTAAACACAAAGCTCATGGCGGCCGGGGAGCGTTCTCCGGCCCGCTTATTCCAGACGAAGCACGGAGAAAGCCGAGGAACGTCGGAAGCAGTAGCTTCGGGAGCTTAGATGAAGTCGAACCCACATCGCCGAAAGTTTCGTGCATGGGACAGATTAAGCACAAGAAACAACAACACCAGCAAATCAAGAAGACAAAACGCGCCGGTTTACCTCCTAGAGATGAATCAAAGCCAGCTTCGTGGTCTCCCCAGGAGATAAAGAAGCACCCGTTTGCGTTTCGGAGGATGTTTAGTAGTGTGAATCCGAGTCGAAAATCCGATGCCGACGGCGATGAGCATAAGAACAAGGCGGCGGGGCTTCCGGATAGAGCGCCGTCGTTGAGCCAGATGCGGCGGTTTGCGAGCGGAAGAGGGCAGGCTTTTGCCAACTTTGATTGGCAAGCGCCGCAGGCCAAGGTGGCGCCACGTCACGATGACGATAACAACCGGAATTATCATTCGGATGAAGATAGAGAATATGGTGATCACGAGCGTGAGTATACCGGAATCGCTTTCTCGGCGCCGTTGACGGTCGGTGGGTCGCCGGGAGGTAACATGGAGTTGCAGCCAAGGAAAGAGATCAATCTATGGAAGAGAAGAACCATGGCTCCGCCTAGTCCTCTACAATTGAAGCCCATGGTTAAAGCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

262

Amino Acids

29.23

Weight (kDa)

10.13

Isoelectric Point (pI)

59.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 556, 655
AccBSI CCGCTC 1 cut(s) 501
AccI GTMKAC 1 cut(s) 635
AccII CGCG 1 cut(s) 297
AccIII TCCGGA 1 cut(s) 457
AcoI YGGCCR 1 cut(s) 118
AcsI RAATTY 1 cut(s) 44
AcyI GRCGYC 2 cut(s) 557, 656
AgsI TTSAA 3 cut(s) 11, 43, 766
AjiI CACGTC 1 cut(s) 563
AjnI CCWGG 1 cut(s) 345
AleI CACNNNNGTG 1 cut(s) 627
AloI GAACNNNNNNTCC 2 cut(s) 727, 759
AluBI AGCT 5 cut(s) 59, 110, 192, 201, 332
AluI AGCT 5 cut(s) 59, 110, 192, 201, 332
Alw26I GTCTC 1 cut(s) 345
Aor13HI TCCGGA 1 cut(s) 457
AoxI GGCC 3 cut(s) 118, 136, 546
ApeKI GCWGC 3 cut(s) 59, 86, 700
ApoI RAATTY 1 cut(s) 44
AspLEI GCGC 6 cut(s) 86, 299, 469, 541, 559, 658
AspS9I GGNCC 1 cut(s) 137
AsuC2I CCSGG 2 cut(s) 122, 681
AsuHPI GGTGA 1 cut(s) 629
BanI GGYRCC 2 cut(s) 556, 655
BauI CACGAG 1 cut(s) 623
BbsI GAAGAC 1 cut(s) 293
BbvI GCAGC 3 cut(s) 46, 73, 712
BceAI ACGGC 3 cut(s) 439, 454, 643
BcgI CGANNNNNNTGC 2 cut(s) 175, 209
BciT130I CCWGG 1 cut(s) 347
BclI TGATCA 1 cut(s) 619
BcnI CCSGG 2 cut(s) 122, 681
BcoDI GTCTC 1 cut(s) 345
BfaI CTAG 2 cut(s) 312, 750
BfoI RGCGCY 5 cut(s) 87, 470, 542, 560, 659
BglI GCCNNNNNGGC 1 cut(s) 554
BisI GCNGC 7 cut(s) 60, 87, 118, 447, 488, 542, 701
BlsI GCNGC 7 cut(s) 61, 88, 119, 448, 489, 543, 702
Bme1390I CCNGG 3 cut(s) 122, 347, 681
BmgBI CACGTC 1 cut(s) 563
BmgT120I GGNCC 1 cut(s) 137
BmiI GGNNCC 3 cut(s) 558, 657, 744
BmrFI CCNGG 3 cut(s) 122, 347, 681
BmsI GCATC 2 cut(s) 406, 474
BpiI GAAGAC 1 cut(s) 293
BpuMI CCSGG 2 cut(s) 122, 681
BsaHI GRCGYC 2 cut(s) 557, 656
BsaI GGTCTC 1 cut(s) 345
BsaJI CCNNGG 7 cut(s) 121, 170, 345, 549, 704, 738, 771
BsaWI WCCGGW 3 cut(s) 457, 582, 638
BsaXI ACNNNNNCTCC 2 cut(s) 727, 757
Bse118I RCCGGY 1 cut(s) 299
BseAI TCCGGA 1 cut(s) 457
BseBI CCWGG 1 cut(s) 347
BseDI CCNNGG 7 cut(s) 121, 170, 345, 549, 704, 738, 771
BseGI GGATG 2 cut(s) 387, 604
BseX3I CGGCCG 1 cut(s) 118
BseXI GCAGC 3 cut(s) 46, 73, 712
Bsh1236I CGCG 1 cut(s) 297
Bsh1285I CGRYCG 2 cut(s) 121, 669
BshFI GGCC 3 cut(s) 120, 138, 548
BshNI GGYRCC 2 cut(s) 556, 655
BsiEI CGRYCG 2 cut(s) 121, 669
BsiSI CCGG 7 cut(s) 121, 135, 300, 458, 583, 639, 680
BslFI GGGAC 1 cut(s) 258
BsmAI GTCTC 1 cut(s) 345
BsmFI GGGAC 1 cut(s) 258
BsnI GGCC 3 cut(s) 120, 138, 548
Bso31I GGTCTC 1 cut(s) 345
Bsp13I TCCGGA 1 cut(s) 457
Bsp143I GATC 2 cut(s) 619, 714
Bsp19I CCATGG 2 cut(s) 738, 771
BspANI GGCC 3 cut(s) 120, 138, 548
BspEI TCCGGA 1 cut(s) 457
BspFNI CGCG 1 cut(s) 297
BspLI GGNNCC 3 cut(s) 558, 657, 744
BspT107I GGYRCC 2 cut(s) 556, 655
BspTNI GGTCTC 1 cut(s) 345
BsrBI CCGCTC 1 cut(s) 501
BsrFI RCCGGY 1 cut(s) 299
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 7 cut(s) 121, 170, 345, 549, 704, 738, 771
BssMI GATC 2 cut(s) 619, 714
BssNAI GTATAC 1 cut(s) 636
BssNI GRCGYC 2 cut(s) 557, 656
BssSI CACGAG 1 cut(s) 623
BssT1I CCWWGG 4 cut(s) 549, 704, 738, 771
Bst1107I GTATAC 1 cut(s) 636
Bst2BI CACGAG 1 cut(s) 623
Bst2UI CCWGG 1 cut(s) 347
Bst4CI ACNGT 1 cut(s) 667
Bst6I CTCTTC 2 cut(s) 499, 722
BstACI GRCGYC 2 cut(s) 557, 656
BstC8I GCNNGC 6 cut(s) 140, 330, 499, 513, 537, 546
BstDEI CTNAG 1 cut(s) 202
BstDSI CCRYGG 2 cut(s) 738, 771
BstF5I GGATG 2 cut(s) 387, 604
BstFNI CGCG 1 cut(s) 297
BstH2I RGCGCY 5 cut(s) 87, 470, 542, 560, 659
BstHHI GCGC 6 cut(s) 86, 299, 469, 541, 559, 658
BstKTI GATC 2 cut(s) 622, 717
BstMAI GTCTC 1 cut(s) 345
BstMBI GATC 2 cut(s) 619, 714
BstMCI CGRYCG 2 cut(s) 121, 669
BstMWI GCNNNNNNNGC 6 cut(s) 65, 198, 541, 545, 554, 653
BstNI CCWGG 1 cut(s) 347
BstSCI CCNGG 3 cut(s) 120, 345, 679
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 3 cut(s) 46, 73, 712
BstV2I GAAGAC 1 cut(s) 293
BstXI CCANNNNNNTGG 1 cut(s) 336
BstZ17I GTATAC 1 cut(s) 636
BstZI CGGCCG 1 cut(s) 118
BsuRI GGCC 3 cut(s) 120, 138, 548
BtgI CCRYGG 2 cut(s) 738, 771
BtgZI GCGATG 2 cut(s) 207, 441
BtrI CACGTC 1 cut(s) 563
BtsCI GGATG 2 cut(s) 387, 604
Cac8I GCNNGC 6 cut(s) 140, 330, 499, 513, 537, 546
CfoI GCGC 6 cut(s) 86, 299, 469, 541, 559, 658
Cfr10I RCCGGY 1 cut(s) 299
Cfr13I GGNCC 1 cut(s) 137
CviAII CATG 6 cut(s) 80, 113, 241, 691, 739, 772
DdeI CTNAG 1 cut(s) 202
DinI GGCGCC 2 cut(s) 558, 657
DpnI GATC 2 cut(s) 621, 716
DpnII GATC 2 cut(s) 619, 714
EaeI YGGCCR 1 cut(s) 118
EagI CGGCCG 1 cut(s) 118
Eam1104I CTCTTC 2 cut(s) 499, 722
EarI CTCTTC 2 cut(s) 499, 722
EciI GGCGGA 1 cut(s) 735
EclXI CGGCCG 1 cut(s) 118
Eco130I CCWWGG 4 cut(s) 549, 704, 738, 771
Eco31I GGTCTC 1 cut(s) 345
Eco52I CGGCCG 1 cut(s) 118
EcoRII CCWGG 1 cut(s) 345
EcoT14I CCWWGG 4 cut(s) 549, 704, 738, 771
EgeI GGCGCC 2 cut(s) 558, 657
EheI GGCGCC 2 cut(s) 558, 657
ErhI CCWWGG 4 cut(s) 549, 704, 738, 771
FaeI CATG 6 cut(s) 83, 116, 244, 694, 742, 775
FaqI GGGAC 1 cut(s) 258
FatI CATG 6 cut(s) 79, 112, 240, 690, 738, 771
FauI CCCGC 2 cut(s) 147, 442
FbaI TGATCA 1 cut(s) 619
FblI GTMKAC 1 cut(s) 635
Fnu4HI GCNGC 7 cut(s) 60, 87, 118, 447, 488, 542, 701
FokI GGATG 2 cut(s) 394, 611
Fsp4HI GCNGC 7 cut(s) 60, 87, 118, 447, 488, 542, 701
FspBI CTAG 2 cut(s) 312, 750
GlaI GCGC 6 cut(s) 85, 298, 468, 540, 558, 657
GluI GCNGC 7 cut(s) 60, 87, 118, 447, 488, 542, 701
HaeII RGCGCY 5 cut(s) 87, 470, 542, 560, 659
HaeIII GGCC 3 cut(s) 120, 138, 548
HapII CCGG 7 cut(s) 121, 135, 300, 458, 583, 639, 680
HhaI GCGC 6 cut(s) 86, 299, 469, 541, 559, 658
Hin1I GRCGYC 2 cut(s) 557, 656
Hin1II CATG 6 cut(s) 83, 116, 244, 694, 742, 775
Hin6I GCGC 6 cut(s) 84, 297, 467, 539, 557, 656
HinP1I GCGC 6 cut(s) 84, 297, 467, 539, 557, 656
HincII GTYRAC 1 cut(s) 663
HindII GTYRAC 1 cut(s) 663
HinfI GANTC 4 cut(s) 320, 397, 403, 642
HpaII CCGG 7 cut(s) 121, 135, 300, 458, 583, 639, 680
HphI GGTGA 1 cut(s) 629
Hpy166II GTNNAC 4 cut(s) 73, 305, 636, 663
Hpy188I TCNGA 5 cut(s) 182, 378, 402, 415, 598
Hpy188III TCNNGA 7 cut(s) 27, 149, 196, 283, 458, 566, 623
Hpy8I GTNNAC 4 cut(s) 73, 305, 636, 663
Hpy99I CGWCG 3 cut(s) 182, 425, 475
HpyCH4III ACNGT 1 cut(s) 667
HpyCH4IV ACGT 2 cut(s) 177, 562
HpyCH4V TGCA 2 cut(s) 240, 700
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 198, 541, 545, 554, 653
HpyF3I CTNAG 1 cut(s) 202
HpySE526I ACGT 2 cut(s) 177, 562
Hsp92I GRCGYC 2 cut(s) 557, 656
Hsp92II CATG 6 cut(s) 83, 116, 244, 694, 742, 775
HspAI GCGC 6 cut(s) 84, 297, 467, 539, 557, 656
KasI GGCGCC 2 cut(s) 556, 655
Kpn2I TCCGGA 1 cut(s) 457
Ksp22I TGATCA 1 cut(s) 619
Kzo9I GATC 2 cut(s) 619, 714
LmnI GCTCC 3 cut(s) 125, 198, 748
Lsp1109I GCAGC 3 cut(s) 46, 73, 712
LweI GCATC 2 cut(s) 406, 474
MaeI CTAG 2 cut(s) 312, 750
MaeII ACGT 2 cut(s) 177, 562
MaeIII GTNAC 2 cut(s) 563, 686
MalI GATC 2 cut(s) 621, 716
MbiI CCGCTC 1 cut(s) 501
MboI GATC 2 cut(s) 619, 714
MboII GAAGA 5 cut(s) 298, 516, 614, 739, 744
MfeI CAATTG 1 cut(s) 761
MluCI AATT 4 cut(s) 44, 586, 761, 784
Mly113I GGCGCC 2 cut(s) 557, 656
MlyI GAGTC 1 cut(s) 412
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 7 cut(s) 73, 165, 318, 372, 500, 677, 765
MroI TCCGGA 1 cut(s) 457
MseI TTAA 2 cut(s) 252, 777
MslI CAYNNNNRTG 2 cut(s) 597, 627
MspI CCGG 7 cut(s) 121, 135, 300, 458, 583, 639, 680
MspR9I CCNGG 3 cut(s) 122, 347, 681
MunI CAATTG 1 cut(s) 761
MvaI CCWGG 1 cut(s) 347
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 6 cut(s) 65, 198, 541, 545, 554, 653
NarI GGCGCC 2 cut(s) 557, 656
NciI CCSGG 2 cut(s) 122, 681
NcoI CCATGG 2 cut(s) 738, 771
NdeII GATC 2 cut(s) 619, 714
NlaIII CATG 6 cut(s) 83, 116, 244, 694, 742, 775
NlaIV GGNNCC 3 cut(s) 558, 657, 744
NmeAIII GCCGAG 2 cut(s) 195, 632
NmuCI GTSAC 1 cut(s) 563
OliI CACNNNNGTG 1 cut(s) 627
PfeI GAWTC 3 cut(s) 320, 397, 642
PkrI GCNGC 7 cut(s) 61, 88, 119, 448, 489, 543, 702
PleI GAGTC 1 cut(s) 411
PluTI GGCGCC 2 cut(s) 560, 659
PpsI GAGTC 1 cut(s) 411
Psp6I CCWGG 1 cut(s) 345
PspGI CCWGG 1 cut(s) 345
PspN4I GGNNCC 3 cut(s) 558, 657, 744
PspPI GGNCC 1 cut(s) 137
RseI CAYNNNNRTG 2 cut(s) 597, 627
SaqAI TTAA 2 cut(s) 252, 777
SatI GCNGC 7 cut(s) 60, 87, 118, 447, 488, 542, 701
Sau3AI GATC 2 cut(s) 619, 714
Sau96I GGNCC 1 cut(s) 137
SchI GAGTC 1 cut(s) 412
ScrFI CCNGG 3 cut(s) 122, 347, 681
SfaNI GCATC 2 cut(s) 406, 474
SfoI GGCGCC 2 cut(s) 558, 657
SmiMI CAYNNNNRTG 2 cut(s) 597, 627
Sse9I AATT 4 cut(s) 44, 586, 761, 784
SspDI GGCGCC 2 cut(s) 556, 655
SspMI CTAG 2 cut(s) 312, 750
StyD4I CCNGG 3 cut(s) 120, 345, 679
StyI CCWWGG 4 cut(s) 549, 704, 738, 771
TaaI ACNGT 1 cut(s) 667
TaiI ACGT 2 cut(s) 180, 565
TaqI TCGA 2 cut(s) 213, 406
TasI AATT 4 cut(s) 44, 586, 761, 784
TauI GCSGC 4 cut(s) 120, 449, 490, 544
TfiI GAWTC 3 cut(s) 320, 397, 642
Tru1I TTAA 2 cut(s) 252, 777
Tru9I TTAA 2 cut(s) 252, 777
TseFI GTSAC 1 cut(s) 563
TseI GCWGC 3 cut(s) 59, 86, 700
Tsp45I GTSAC 1 cut(s) 563
TspDTI ATGAA 4 cut(s) 68, 222, 333, 615
TspGWI ACGGA 1 cut(s) 175
XapI RAATTY 1 cut(s) 44
XmiI GTMKAC 1 cut(s) 635
XspI CTAG 2 cut(s) 312, 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.