Rroxscaffold_2G00144080

At1g76070-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008592
Physical Location & Seq
Forward (+)
82035198 .. 82035833
636 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00144080.1

Sequence Viewer

Length: 636 bp
ATGGAGAGGCCATCGAAACTGAAAATCAAAATCTTGAAGTTTCTATCTCAAGCTGCAGCGCCTGCAGTCACCTACAAAAGCCCGCCTGCTAGTCCTCGGAATCCTATCGTATCAATCATTCCGAAAGAGGCTAGGAGAAAGCCGAGGAATGGATCCAGTAGTTTTGCGTCAAAAGAACCCACTTCCCCAAAGGTTTCTTGCATGGGTCAAGTCAAAGGCAAGAAGAAGAAAAGGAAGAGTAAATCCAAGCCTAAGCAGGTCGGCCCTCCACCACCACCACCACAAGAGACCACCAGTCTGGCTCTCTCTGTTCCTAAAGAGAAGACTTTCAAGGTTTCAAATGTCATCCCAAAGGTGCTCATAAACAATAAAGGTAAGACTAAGGATGACGACGGTTCGGTAGCTACAAGCTCATTATCTAGAACTAGTCGTGTTCCAGAAAGAGTGCCTTCATTGGGTCAGATGAAGAGGTTTGAGAGTGGCCGCGGTGTGTTGTCTGATGATCCTCATCGTTCAGACAACTTGAAAATTGGGGTAGGTGGAGATGAAGAATATGATGATGAGGATGAGAAGAAAGAAGTAATTATGCTGTGGAAAAGAAGAAGCATGTCTCCTCCTATGCCTCTTCAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

211

Amino Acids

23.22

Weight (kDa)

10.21

Isoelectric Point (pI)

63.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 247
AccII CGCG 1 cut(s) 486
AciI CCGC 3 cut(s) 83, 484, 486
AclWI GGATC 3 cut(s) 147, 160, 497
AcoI YGGCCR 1 cut(s) 481
AcuI CTGAAG 1 cut(s) 611
AfiI CCNNNNNNNGG 2 cut(s) 149, 455
AgsI TTSAA 4 cut(s) 37, 331, 339, 526
AhdI GACNNNNNGTC 1 cut(s) 294
AhlI ACTAGT 1 cut(s) 425
AluBI AGCT 4 cut(s) 53, 404, 411, 631
AluI AGCT 4 cut(s) 53, 404, 411, 631
Alw21I GWGCWC 1 cut(s) 360
Alw26I GTCTC 2 cut(s) 281, 615
AlwI GGATC 3 cut(s) 147, 160, 497
AlwNI CAGNNNCTG 1 cut(s) 62
AoxI GGCC 3 cut(s) 8, 262, 481
ApeKI GCWGC 2 cut(s) 53, 56
Asp700I GAANNNNTTC 1 cut(s) 326
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 1 cut(s) 263
AsuHPI GGTGA 1 cut(s) 61
BamHI GGATCC 1 cut(s) 152
BbsI GAAGAC 1 cut(s) 329
Bbv12I GWGCWC 1 cut(s) 360
BbvI GCAGC 2 cut(s) 40, 68
BccI CCATC 1 cut(s) 19
BcoDI GTCTC 2 cut(s) 281, 615
BcuI ACTAGT 1 cut(s) 425
BfaI CTAG 4 cut(s) 90, 132, 420, 426
BfmI CTRYAG 2 cut(s) 54, 63
BfoI RGCGCY 1 cut(s) 62
BfuAI ACCTGC 1 cut(s) 247
BisI GCNGC 3 cut(s) 54, 57, 484
BlsI GCNGC 3 cut(s) 55, 58, 485
BmeRI GACNNNNNGTC 1 cut(s) 294
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 1 cut(s) 154
BpiI GAAGAC 1 cut(s) 329
Bpu10I CCTNAGC 1 cut(s) 252
BpuEI CTTGAG 1 cut(s) 33
BsaBI GATNNNNATC 1 cut(s) 507
BsaI GGTCTC 1 cut(s) 281
BsaJI CCNNGG 3 cut(s) 95, 143, 484
Bsc4I CCNNNNNNNGG 2 cut(s) 149, 455
Bse1I ACTGG 2 cut(s) 156, 294
Bse8I GATNNNNATC 1 cut(s) 507
BseDI CCNNGG 3 cut(s) 95, 143, 484
BseGI GGATG 3 cut(s) 345, 391, 571
BseJI GATNNNNATC 1 cut(s) 507
BseLI CCNNNNNNNGG 2 cut(s) 149, 455
BseNI ACTGG 2 cut(s) 156, 294
BseRI GAGGAG 1 cut(s) 603
BseXI GCAGC 2 cut(s) 40, 68
Bsh1236I CGCG 1 cut(s) 486
BshFI GGCC 3 cut(s) 10, 264, 483
BsiHKAI GWGCWC 1 cut(s) 360
BslI CCNNNNNNNGG 2 cut(s) 149, 455
BsmAI GTCTC 2 cut(s) 281, 615
BsnI GGCC 3 cut(s) 10, 264, 483
Bso31I GGTCTC 1 cut(s) 281
Bsp1286I GDGCHC 1 cut(s) 360
Bsp143I GATC 2 cut(s) 152, 502
BspACI CCGC 3 cut(s) 83, 484, 486
BspANI GGCC 3 cut(s) 10, 264, 483
BspFNI CGCG 1 cut(s) 486
BspLI GGNNCC 1 cut(s) 154
BspMAI CTGCAG 2 cut(s) 58, 67
BspMI ACCTGC 1 cut(s) 247
BspPI GGATC 3 cut(s) 147, 160, 497
BspTNI GGTCTC 1 cut(s) 281
BsrI ACTGG 2 cut(s) 156, 294
BssECI CCNNGG 3 cut(s) 95, 143, 484
BssMI GATC 2 cut(s) 152, 502
Bst4CI ACNGT 1 cut(s) 395
Bst6I CTCTTC 3 cut(s) 230, 461, 630
BstAPI GCANNNNNTGC 1 cut(s) 62
BstC8I GCNNGC 3 cut(s) 63, 83, 87
BstDEI CTNAG 2 cut(s) 252, 381
BstDSI CCRYGG 1 cut(s) 484
BstF5I GGATG 3 cut(s) 345, 391, 571
BstFNI CGCG 1 cut(s) 486
BstH2I RGCGCY 1 cut(s) 62
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 2 cut(s) 155, 505
BstMAI GTCTC 2 cut(s) 281, 615
BstMBI GATC 2 cut(s) 152, 502
BstMWI GCNNNNNNNGC 2 cut(s) 62, 628
BstNSI RCATGY 1 cut(s) 610
BstSFI CTRYAG 2 cut(s) 54, 63
BstUI CGCG 1 cut(s) 486
BstV1I GCAGC 2 cut(s) 40, 68
BstV2I GAAGAC 1 cut(s) 329
BstX2I RGATCY 1 cut(s) 152
BstXI CCANNNNNNTGG 1 cut(s) 298
BstYI RGATCY 1 cut(s) 152
BsuRI GGCC 3 cut(s) 10, 264, 483
BtgI CCRYGG 1 cut(s) 484
BtsCI GGATG 3 cut(s) 345, 391, 571
BveI ACCTGC 1 cut(s) 247
Cac8I GCNNGC 3 cut(s) 63, 83, 87
CaiI CAGNNNCTG 1 cut(s) 62
CfoI GCGC 1 cut(s) 61
Cfr13I GGNCC 1 cut(s) 263
Cfr42I CCGCGG 1 cut(s) 487
CseI GACGC 1 cut(s) 156
CviAII CATG 2 cut(s) 202, 607
DdeI CTNAG 2 cut(s) 252, 381
DpnI GATC 2 cut(s) 154, 504
DpnII GATC 2 cut(s) 152, 502
DriI GACNNNNNGTC 1 cut(s) 294
EaeI YGGCCR 1 cut(s) 481
Eam1104I CTCTTC 3 cut(s) 230, 461, 630
Eam1105I GACNNNNNGTC 1 cut(s) 294
EarI CTCTTC 3 cut(s) 230, 461, 630
Eco31I GGTCTC 1 cut(s) 281
Eco57I CTGAAG 1 cut(s) 611
FaeI CATG 2 cut(s) 205, 610
FaiI YATR 6 cut(s) 203, 362, 555, 587, 608, 620
FalI AAGNNNNNCTT 2 cut(s) 433, 465
FatI CATG 2 cut(s) 201, 606
FauI CCCGC 1 cut(s) 90
Fnu4HI GCNGC 3 cut(s) 54, 57, 484
FokI GGATG 3 cut(s) 332, 398, 578
Fsp4HI GCNGC 3 cut(s) 54, 57, 484
FspBI CTAG 4 cut(s) 90, 132, 420, 426
GlaI GCGC 1 cut(s) 60
GluI GCNGC 3 cut(s) 54, 57, 484
HaeII RGCGCY 1 cut(s) 62
HaeIII GGCC 3 cut(s) 10, 264, 483
HgaI GACGC 1 cut(s) 156
HhaI GCGC 1 cut(s) 61
Hin1II CATG 2 cut(s) 205, 610
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HinfI GANTC 1 cut(s) 100
HphI GGTGA 1 cut(s) 61
Hpy188I TCNGA 5 cut(s) 99, 123, 462, 499, 517
Hpy188III TCNNGA 3 cut(s) 34, 420, 437
Hpy99I CGWCG 1 cut(s) 395
HpyAV CCTTC 1 cut(s) 459
HpyCH4III ACNGT 1 cut(s) 395
HpyCH4V TGCA 3 cut(s) 56, 65, 201
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 628
HpyF3I CTNAG 2 cut(s) 252, 381
Hsp92II CATG 2 cut(s) 205, 610
HspAI GCGC 1 cut(s) 59
KspI CCGCGG 1 cut(s) 487
Kzo9I GATC 2 cut(s) 152, 502
LpnPI CCDG 7 cut(s) 75, 99, 169, 242, 284, 307, 450
Lsp1109I GCAGC 2 cut(s) 40, 68
MaeI CTAG 4 cut(s) 90, 132, 420, 426
MaeIII GTNAC 1 cut(s) 67
MalI GATC 2 cut(s) 154, 504
MboI GATC 2 cut(s) 152, 502
MboII GAAGA 9 cut(s) 235, 238, 247, 334, 478, 560, 583, 612, 617
MflI RGATCY 1 cut(s) 152
MhlI GDGCHC 1 cut(s) 360
MluCI AATT 2 cut(s) 528, 582
MnlI CCTC 9 cut(s) 105, 121, 138, 276, 462, 516, 556, 624, 633
MroXI GAANNNNTTC 1 cut(s) 326
MspA1I CMGCKG 2 cut(s) 486, 631
MvnI CGCG 1 cut(s) 486
MwoI GCNNNNNNNGC 2 cut(s) 62, 628
NdeII GATC 2 cut(s) 152, 502
NlaIII CATG 2 cut(s) 205, 610
NlaIV GGNNCC 1 cut(s) 154
NmeAIII GCCGAG 1 cut(s) 168
NmuCI GTSAC 1 cut(s) 67
NspI RCATGY 1 cut(s) 610
PdmI GAANNNNTTC 1 cut(s) 326
PfeI GAWTC 1 cut(s) 100
PkrI GCNGC 3 cut(s) 55, 58, 485
PspN4I GGNNCC 1 cut(s) 154
PspPI GGNCC 1 cut(s) 263
PstI CTGCAG 2 cut(s) 58, 67
PstNI CAGNNNCTG 1 cut(s) 62
PsuI RGATCY 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 631
SacII CCGCGG 1 cut(s) 487
SatI GCNGC 3 cut(s) 54, 57, 484
Sau3AI GATC 2 cut(s) 152, 502
Sau96I GGNCC 1 cut(s) 263
SduI GDGCHC 1 cut(s) 360
SfcI CTRYAG 2 cut(s) 54, 63
Sfr303I CCGCGG 1 cut(s) 487
SgrBI CCGCGG 1 cut(s) 487
SmlI CTYRAG 1 cut(s) 48
SmoI CTYRAG 1 cut(s) 48
SpeI ACTAGT 1 cut(s) 425
Sse9I AATT 2 cut(s) 528, 582
SsiI CCGC 3 cut(s) 83, 484, 486
SspMI CTAG 4 cut(s) 90, 132, 420, 426
TaaI ACNGT 1 cut(s) 395
TaqI TCGA 1 cut(s) 14
TasI AATT 2 cut(s) 528, 582
TauI GCSGC 1 cut(s) 486
TfiI GAWTC 1 cut(s) 100
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 2 cut(s) 53, 56
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 3 cut(s) 441, 479, 561
XbaI TCTAGA 1 cut(s) 419
XceI RCATGY 1 cut(s) 610
XmnI GAANNNNTTC 1 cut(s) 326
XspI CTAG 4 cut(s) 90, 132, 420, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.