Rorug02G0071900

At1g76070-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008592
Physical Location & Seq
Forward (+)
5670892 .. 5672061
1170 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0071900.1

Sequence Viewer

Length: 1170 bp
ATGGAAGACCGTGGCCAAAACGTCGACCTTCCACCCGAGATCATATATGAGATTCTTTCAAGGTTACCAGTGAAACCCTTGTACCGTTTCAAGTGTGTATCAAAGCCATGGCGGCCTCTAATCTCCCATCCTGATTTCGTCGCAAACTATTCCAAAGCCATTGAGAATAAGGACGTCTTCTTCAGAAGACGGCGCCTCCTTTTTACTCTTTATGACCCGCCAAAACGGCTATACTCTTTGAATCTTGACCAGTTTCTCAATGAGAATCATAATGTTGACGTTGATAGTTTAGTAGCAACGCCCACTGAGCTCGACTTTGTTTACAATCATATTCCAACAGGTCAACTGGGTTGGATTCCCTTTGTCCATTGTTCCTGCTATGGCTTGTTCTTGTCCCTTGTAGCTTCTGAAGGGTGTTACAGCGTCAGTTTGATTAACCCTGTAACCAAGGAATCGAAGACACTAGAAAAGGCACCAATAGAGAGATCAGAACGAGTGACGCGCTACCCTCGGCACTTATATGGACTTGGATTTGATTACTCCACTAACGAATACAAGGTAATTCACGTGCGGGATTATCATCATGGAACTGGTTTTTGTGTCTATACATTGCAGACTGATTCTTGGCGACAGATTGACCCCTTATTTCCTTACAACCTTTTACCTTATGATGGGATCCTGGTGAATGGTGGTGTTCATTGGTTGGTGACTAAGGTTGCAGATAACTCGTTGGTGATTATGTCTTTTCTTTTAGCAGAGGAGGAGGTTAGAGAAATTGCACTACCGCCCAATGCCACTAGTTCAATTCGACTAGTGGCGTTCAGAAATTGGCTATGTATAACACCAGAATCACCAAAGAATGATGAAGTAACATTTAATGAGTTTTGGATCATGAAGGAATACGGAGTGAGGGAGTCTTGGACTAAAATGCAGGTCTCCATCCCATTTCACCGGTTATCACATTCTGGCTTTTGGACAGAAACTTATGATTTGATGGTGTTTGATGATTCGTTAGTTATGTACAATTTTGATGATGGAACATTCTGGAATCTATCAATTGGTAATACTAGCGAAAATCACTTTGGTACCGTTGGGATCTATTTGGAGAGCCTACCTTCACTCAATCGATCAAGAACAACAAGACCTAAGGAAGATGAATCCACTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

389

Amino Acids

45.23

Weight (kDa)

5.67

Isoelectric Point (pI)

43.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 9 - 46 3.6e-08 F-box domain
F-box-like PF12937 9 - 44 3e-06 F-box-like
FBA_1 PF07734 124 - 371 3.6e-18 F-box associated beta propeller domain
FBA_3 PF08268 131 - 318 2.1e-17 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 177
Acc36I ACCTGC 1 cut(s) 922
Acc65I GGTACC 1 cut(s) 1085
AccB1I GGYRCC 3 cut(s) 192, 472, 1085
AccI GTMKAC 1 cut(s) 24
AccII CGCG 1 cut(s) 502
AciI CCGC 4 cut(s) 112, 218, 571, 785
AclWI GGATC 4 cut(s) 670, 683, 896, 1103
AcoI YGGCCR 1 cut(s) 13
AcuI CTGAAG 2 cut(s) 166, 429
AcvI CACGTG 1 cut(s) 568
AcyI GRCGYC 2 cut(s) 174, 193
AdeI CACNNNGTG 1 cut(s) 1167
AfaI GTAC 3 cut(s) 83, 1022, 1087
AfiI CCNNNNNNNGG 1 cut(s) 671
AgeI ACCGGT 1 cut(s) 951
AgsI TTSAA 4 cut(s) 60, 91, 241, 804
AhlI ACTAGT 2 cut(s) 797, 811
AjnI CCWGG 1 cut(s) 678
AjuI GAANNNNNNNTTGG 2 cut(s) 1065, 1097
AluBI AGCT 2 cut(s) 310, 404
AluI AGCT 2 cut(s) 310, 404
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 1 cut(s) 940
AlwI GGATC 4 cut(s) 670, 683, 896, 1103
Ama87I CYCGRG 1 cut(s) 35
AoxI GGCC 2 cut(s) 13, 113
AsiGI ACCGGT 1 cut(s) 951
Asp718I GGTACC 1 cut(s) 1085
AspLEI GCGC 2 cut(s) 195, 504
AsuHPI GGTGA 5 cut(s) 694, 718, 745, 843, 941
AvaI CYCGRG 1 cut(s) 35
AxyI CCTNAGG 1 cut(s) 1146
BaeI ACNNNNGTAYC 4 cut(s) 65, 98, 1069, 1102
BalI TGGCCA 1 cut(s) 15
BamHI GGATCC 1 cut(s) 675
BanI GGYRCC 3 cut(s) 192, 472, 1085
BanII GRGCYC 1 cut(s) 312
BbrPI CACGTG 1 cut(s) 568
BbsI GAAGAC 4 cut(s) 12, 169, 193, 464
Bbv12I GWGCWC 1 cut(s) 312
BccI CCATC 5 cut(s) 135, 665, 947, 988, 1028
BceAI ACGGC 2 cut(s) 206, 242
BcgI CGANNNNNNTGC 2 cut(s) 708, 742
BciT130I CCWGG 1 cut(s) 680
BcoDI GTCTC 1 cut(s) 940
BcuI ACTAGT 2 cut(s) 797, 811
BfaI CTAG 4 cut(s) 464, 798, 812, 1068
BfoI RGCGCY 1 cut(s) 196
BfuAI ACCTGC 1 cut(s) 922
BglI GCCNNNNNGGC 2 cut(s) 112, 226
BisI GCNGC 1 cut(s) 113
BlsI GCNGC 1 cut(s) 114
Bme1390I CCNGG 1 cut(s) 680
BmeT110I CYCGRG 1 cut(s) 35
BmiI GGNNCC 4 cut(s) 194, 474, 677, 1087
BmrFI CCNGG 1 cut(s) 680
BmrI ACTGGG 1 cut(s) 356
BmuI ACTGGG 1 cut(s) 356
BpiI GAAGAC 4 cut(s) 12, 169, 193, 464
Bsa29I ATCGAT 1 cut(s) 1126
BsaAI YACGTR 1 cut(s) 568
BsaBI GATNNNNATC 1 cut(s) 579
BsaHI GRCGYC 2 cut(s) 174, 193
BsaI GGTCTC 1 cut(s) 940
BsaJI CCNNGG 4 cut(s) 10, 107, 447, 509
BsaWI WCCGGW 1 cut(s) 951
BsaXI ACNNNNNCTCC 2 cut(s) 180, 210
Bsc4I CCNNNNNNNGG 1 cut(s) 671
Bse118I RCCGGY 1 cut(s) 951
Bse1I ACTGG 4 cut(s) 68, 250, 351, 595
Bse21I CCTNAGG 1 cut(s) 1146
Bse3DI GCAATG 1 cut(s) 608
Bse8I GATNNNNATC 1 cut(s) 579
BseBI CCWGG 1 cut(s) 680
BseCI ATCGAT 1 cut(s) 1126
BseDI CCNNGG 4 cut(s) 10, 107, 447, 509
BseGI GGATG 2 cut(s) 127, 939
BseJI GATNNNNATC 1 cut(s) 579
BseLI CCNNNNNNNGG 1 cut(s) 671
BseMI GCAATG 1 cut(s) 608
BseMII CTCAG 1 cut(s) 297
BseNI ACTGG 4 cut(s) 68, 250, 351, 595
BseRI GAGGAG 2 cut(s) 773, 776
Bsh1236I CGCG 1 cut(s) 502
BshFI GGCC 2 cut(s) 15, 115
BshNI GGYRCC 3 cut(s) 192, 472, 1085
BshTI ACCGGT 1 cut(s) 951
BshVI ATCGAT 1 cut(s) 1126
BsiHKAI GWGCWC 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 35
BsiSI CCGG 1 cut(s) 952
BslFI GGGAC 1 cut(s) 379
BslI CCNNNNNNNGG 1 cut(s) 671
BsmAI GTCTC 1 cut(s) 940
BsmFI GGGAC 1 cut(s) 379
BsnI GGCC 2 cut(s) 15, 115
Bso31I GGTCTC 1 cut(s) 940
BsoBI CYCGRG 1 cut(s) 35
Bsp1286I GDGCHC 1 cut(s) 312
Bsp1407I TGTACA 1 cut(s) 1020
Bsp143I GATC 6 cut(s) 39, 485, 675, 888, 1095, 1127
Bsp19I CCATGG 1 cut(s) 107
BspACI CCGC 4 cut(s) 112, 218, 571, 785
BspANI GGCC 2 cut(s) 15, 115
BspCNI CTCAG 1 cut(s) 298
BspDI ATCGAT 1 cut(s) 1126
BspFNI CGCG 1 cut(s) 502
BspHI TCATGA 1 cut(s) 891
BspLI GGNNCC 4 cut(s) 194, 474, 677, 1087
BspMI ACCTGC 1 cut(s) 922
BspPI GGATC 4 cut(s) 670, 683, 896, 1103
BspT107I GGYRCC 3 cut(s) 192, 472, 1085
BspTNI GGTCTC 1 cut(s) 940
BsrDI GCAATG 1 cut(s) 608
BsrFI RCCGGY 1 cut(s) 951
BsrGI TGTACA 1 cut(s) 1020
BsrI ACTGG 4 cut(s) 68, 250, 351, 595
BssAI RCCGGY 1 cut(s) 951
BssECI CCNNGG 4 cut(s) 10, 107, 447, 509
BssMI GATC 6 cut(s) 39, 485, 675, 888, 1095, 1127
BssNI GRCGYC 2 cut(s) 174, 193
BssT1I CCWWGG 2 cut(s) 107, 447
Bst2UI CCWGG 1 cut(s) 680
Bst4CI ACNGT 3 cut(s) 11, 86, 1090
BstACI GRCGYC 2 cut(s) 174, 193
BstAUI TGTACA 1 cut(s) 1020
BstBAI YACGTR 1 cut(s) 568
BstDEI CTNAG 4 cut(s) 306, 711, 1146, 1164
BstDSI CCRYGG 2 cut(s) 10, 107
BstEII GGTNACC 1 cut(s) 63
BstF5I GGATG 2 cut(s) 127, 939
BstFNI CGCG 1 cut(s) 502
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 2 cut(s) 195, 504
BstKTI GATC 6 cut(s) 42, 488, 678, 891, 1098, 1130
BstMAI GTCTC 1 cut(s) 940
BstMBI GATC 6 cut(s) 39, 485, 675, 888, 1095, 1127
BstMWI GCNNNNNNNGC 3 cut(s) 112, 226, 307
BstNI CCWGG 1 cut(s) 680
BstPI GGTNACC 1 cut(s) 63
BstSCI CCNGG 1 cut(s) 678
BstUI CGCG 1 cut(s) 502
BstV2I GAAGAC 4 cut(s) 12, 169, 193, 464
BstX2I RGATCY 2 cut(s) 675, 1095
BstYI RGATCY 2 cut(s) 675, 1095
Bsu15I ATCGAT 1 cut(s) 1126
Bsu36I CCTNAGG 1 cut(s) 1146
BsuRI GGCC 2 cut(s) 15, 115
BsuTUI ATCGAT 1 cut(s) 1126
BtgI CCRYGG 2 cut(s) 10, 107
BtsCI GGATG 2 cut(s) 127, 939
BtsIMutI CAGTG 2 cut(s) 75, 303
BveI ACCTGC 1 cut(s) 922
CciI TCATGA 1 cut(s) 891
CfoI GCGC 2 cut(s) 195, 504
Cfr10I RCCGGY 1 cut(s) 951
ClaI ATCGAT 1 cut(s) 1126
CseI GACGC 2 cut(s) 412, 508
Csp6I GTAC 3 cut(s) 82, 1021, 1086
CspAI ACCGGT 1 cut(s) 951
CviAII CATG 3 cut(s) 108, 584, 892
CviQI GTAC 3 cut(s) 82, 1021, 1086
DdeI CTNAG 4 cut(s) 306, 711, 1146, 1164
DinI GGCGCC 1 cut(s) 194
DpnI GATC 6 cut(s) 41, 487, 677, 890, 1097, 1129
DpnII GATC 6 cut(s) 39, 485, 675, 888, 1095, 1127
DraIII CACNNNGTG 1 cut(s) 1167
EaeI YGGCCR 1 cut(s) 13
Ecl136II GAGCTC 1 cut(s) 310
Eco130I CCWWGG 2 cut(s) 107, 447
Eco24I GRGCYC 1 cut(s) 312
Eco31I GGTCTC 1 cut(s) 940
Eco53kI GAGCTC 1 cut(s) 310
Eco57I CTGAAG 2 cut(s) 166, 429
Eco72I CACGTG 1 cut(s) 568
Eco81I CCTNAGG 1 cut(s) 1146
Eco88I CYCGRG 1 cut(s) 35
Eco91I GGTNACC 1 cut(s) 63
EcoICRI GAGCTC 1 cut(s) 310
EcoO65I GGTNACC 1 cut(s) 63
EcoRII CCWGG 1 cut(s) 678
EcoT14I CCWWGG 2 cut(s) 107, 447
EcoT38I GRGCYC 1 cut(s) 312
EgeI GGCGCC 1 cut(s) 194
EheI GGCGCC 1 cut(s) 194
ErhI CCWWGG 2 cut(s) 107, 447
FaeI CATG 3 cut(s) 111, 587, 895
FalI AAGNNNNNCTT 2 cut(s) 161, 193
FaqI GGGAC 1 cut(s) 379
FatI CATG 3 cut(s) 107, 583, 891
FauI CCCGC 2 cut(s) 225, 564
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 1 cut(s) 113
FokI GGATG 2 cut(s) 114, 926
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 1 cut(s) 113
FspBI CTAG 4 cut(s) 464, 798, 812, 1068
GlaI GCGC 2 cut(s) 194, 503
GluI GCNGC 1 cut(s) 113
HaeII RGCGCY 1 cut(s) 196
HaeIII GGCC 2 cut(s) 15, 115
HapII CCGG 1 cut(s) 952
HgaI GACGC 2 cut(s) 412, 508
HhaI GCGC 2 cut(s) 195, 504
Hin1I GRCGYC 2 cut(s) 174, 193
Hin1II CATG 3 cut(s) 111, 587, 895
Hin6I GCGC 2 cut(s) 193, 502
HinP1I GCGC 2 cut(s) 193, 502
HincII GTYRAC 3 cut(s) 25, 277, 344
HindII GTYRAC 3 cut(s) 25, 277, 344
HpaII CCGG 1 cut(s) 952
HphI GGTGA 5 cut(s) 694, 718, 745, 843, 941
Hpy166II GTNNAC 4 cut(s) 25, 277, 322, 344
Hpy188I TCNGA 4 cut(s) 185, 409, 490, 824
Hpy188III TCNNGA 5 cut(s) 131, 245, 892, 1045, 1131
Hpy8I GTNNAC 4 cut(s) 25, 277, 322, 344
Hpy99I CGWCG 2 cut(s) 26, 143
HpyAV CCTTC 4 cut(s) 38, 404, 889, 1125
HpyCH4III ACNGT 3 cut(s) 11, 86, 1090
HpyCH4IV ACGT 4 cut(s) 21, 174, 279, 567
HpyCH4V TGCA 4 cut(s) 613, 719, 779, 931
HpyF10VI GCNNNNNNNGC 3 cut(s) 112, 226, 307
HpyF3I CTNAG 4 cut(s) 306, 711, 1146, 1164
HpySE526I ACGT 4 cut(s) 21, 174, 279, 567
Hsp92I GRCGYC 2 cut(s) 174, 193
Hsp92II CATG 3 cut(s) 111, 587, 895
HspAI GCGC 2 cut(s) 193, 502
KasI GGCGCC 1 cut(s) 192
KpnI GGTACC 1 cut(s) 1089
Kzo9I GATC 6 cut(s) 39, 485, 675, 888, 1095, 1127
MaeI CTAG 4 cut(s) 464, 798, 812, 1068
MaeII ACGT 4 cut(s) 21, 174, 279, 567
MaeIII GTNAC 6 cut(s) 63, 416, 442, 496, 706, 868
MalI GATC 6 cut(s) 41, 487, 677, 890, 1097, 1129
MboI GATC 6 cut(s) 39, 485, 675, 888, 1095, 1127
MboII GAAGA 6 cut(s) 17, 169, 172, 198, 469, 1163
MfeI CAATTG 1 cut(s) 1056
MflI RGATCY 2 cut(s) 675, 1095
MhlI GDGCHC 1 cut(s) 312
MlsI TGGCCA 1 cut(s) 15
MluCI AATT 6 cut(s) 561, 774, 804, 826, 1024, 1056
MluNI TGGCCA 1 cut(s) 15
Mly113I GGCGCC 1 cut(s) 193
MlyI GAGTC 1 cut(s) 923
MmeI TCCRAC 2 cut(s) 332, 359
MnlI CCTC 7 cut(s) 126, 206, 519, 751, 754, 757, 903
Mox20I TGGCCA 1 cut(s) 15
MscI TGGCCA 1 cut(s) 15
MseI TTAA 2 cut(s) 435, 876
MslI CAYNNNNRTG 1 cut(s) 519
Msp20I TGGCCA 1 cut(s) 15
MspI CCGG 1 cut(s) 952
MspR9I CCNGG 1 cut(s) 680
MunI CAATTG 1 cut(s) 1056
MvaI CCWGG 1 cut(s) 680
MvnI CGCG 1 cut(s) 502
MwoI GCNNNNNNNGC 3 cut(s) 112, 226, 307
NarI GGCGCC 1 cut(s) 193
NcoI CCATGG 1 cut(s) 107
NdeII GATC 6 cut(s) 39, 485, 675, 888, 1095, 1127
NlaIII CATG 3 cut(s) 111, 587, 895
NlaIV GGNNCC 4 cut(s) 194, 474, 677, 1087
NmeAIII GCCGAG 1 cut(s) 490
NmuCI GTSAC 2 cut(s) 496, 706
PagI TCATGA 1 cut(s) 891
PinAI ACCGGT 1 cut(s) 951
PkrI GCNGC 1 cut(s) 114
PleI GAGTC 1 cut(s) 922
PluTI GGCGCC 1 cut(s) 196
PmaCI CACGTG 1 cut(s) 568
PmlI CACGTG 1 cut(s) 568
PpsI GAGTC 1 cut(s) 922
Ppu21I YACGTR 1 cut(s) 568
Psp124BI GAGCTC 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 678
PspCI CACGTG 1 cut(s) 568
PspEI GGTNACC 1 cut(s) 63
PspGI CCWGG 1 cut(s) 678
PspN4I GGNNCC 4 cut(s) 194, 474, 677, 1087
PsuI RGATCY 2 cut(s) 675, 1095
RsaI GTAC 3 cut(s) 83, 1022, 1087
RsaNI GTAC 3 cut(s) 82, 1021, 1086
RseI CAYNNNNRTG 1 cut(s) 519
SacI GAGCTC 1 cut(s) 312
SalI GTCGAC 1 cut(s) 23
SaqAI TTAA 2 cut(s) 435, 876
SatI GCNGC 1 cut(s) 113
Sau3AI GATC 6 cut(s) 39, 485, 675, 888, 1095, 1127
SchI GAGTC 1 cut(s) 923
ScrFI CCNGG 1 cut(s) 680
SduI GDGCHC 1 cut(s) 312
SfoI GGCGCC 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 519
SpeI ACTAGT 2 cut(s) 797, 811
Sse9I AATT 6 cut(s) 561, 774, 804, 826, 1024, 1056
SsiI CCGC 4 cut(s) 112, 218, 571, 785
SspDI GGCGCC 1 cut(s) 192
SspMI CTAG 4 cut(s) 464, 798, 812, 1068
SstI GAGCTC 1 cut(s) 312
StyD4I CCNGG 1 cut(s) 678
StyI CCWWGG 2 cut(s) 107, 447
TaaI ACNGT 3 cut(s) 11, 86, 1090
TaiI ACGT 4 cut(s) 24, 177, 282, 570
TaqI TCGA 5 cut(s) 24, 312, 455, 808, 1126
TasI AATT 6 cut(s) 561, 774, 804, 826, 1024, 1056
TatI WGTACW 1 cut(s) 1020
TauI GCSGC 1 cut(s) 115
Tru1I TTAA 2 cut(s) 435, 876
Tru9I TTAA 2 cut(s) 435, 876
TscAI CASTG 2 cut(s) 75, 310
TseFI GTSAC 2 cut(s) 496, 706
Tsp45I GTSAC 2 cut(s) 496, 706
TspDTI ATGAA 4 cut(s) 686, 879, 908, 1170
TspGWI ACGGA 1 cut(s) 918
TspRI CASTG 2 cut(s) 75, 310
XmiI GTMKAC 1 cut(s) 24
XspI CTAG 4 cut(s) 464, 798, 812, 1068
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.