Calcium-binding EF hand family protein
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G20760 |
| fragaria_vesca | FvH4_5g25580 FvH4_5g25580 |
| malus_domestica | MD06G1007400.v1.1 |
| prunus_persica | Prupe.5G009300_v2.0.a1 Prupe.5G009300_v2.0.a1 |
| pyrus_communis | pycom06g00650 pycom06g00660 |
| rosa_chinensis | RchiOBHm_Chr7g0215891 RchiOBHm_Chr7g0219801 RchiOBHm_Chr7g0219941 |
| rosa_laevigata | RLG00000002612 |
| rosa_multiflora | Rmu_co8383945.1_g000001 Rmu_sc0006240.1_g000011 |
| rosa_roxburghii | Rroxscaffold_3G00243830 |
| rosa_rugosa | Rorug07G0156400 Rorug07G0156500 |
| rosa_samantha | Rh7AG294500 Rh7AG298200 Rh7BG286300 Rh7CG314500 Rh7CG318200 Rh7CG318300 Rh7DG297400 |
| rosa_wichuraiana | Rw7G025020 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 198 |
| AarI | CACCTGC | 1 cut(s) | 1701 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 2333 |
| AatII | GACGTC | 1 cut(s) | 2838 |
| Acc36I | ACCTGC | 2 cut(s) | 118, 1701 |
| AccB1I | GGYRCC | 3 cut(s) | 83, 2561, 2720 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 494, 1451 |
| AccBSI | CCGCTC | 1 cut(s) | 1276 |
| AccI | GTMKAC | 2 cut(s) | 1501, 2040 |
| AccII | CGCG | 1 cut(s) | 1432 |
| AciI | CCGC | 8 cut(s) | 5, 491, 654, 1276, 1305, 1432, 1870, 2272 |
| AclI | AACGTT | 1 cut(s) | 611 |
| AclWI | GGATC | 7 cut(s) | 33, 83, 118, 722, 1021, 1399, 2506 |
| AcoI | YGGCCR | 3 cut(s) | 1018, 1055, 1433 |
| AcsI | RAATTY | 4 cut(s) | 2067, 2386, 2393, 2852 |
| AcuI | CTGAAG | 3 cut(s) | 897, 911, 1919 |
| AcyI | GRCGYC | 3 cut(s) | 2562, 2721, 2835 |
| AdeI | CACNNNGTG | 1 cut(s) | 170 |
| AfaI | GTAC | 5 cut(s) | 895, 1410, 1703, 2191, 2932 |
| AflIII | ACRYGT | 1 cut(s) | 765 |
| AhlI | ACTAGT | 1 cut(s) | 584 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1997, 2029 |
| AlwI | GGATC | 7 cut(s) | 33, 83, 118, 722, 1021, 1399, 2506 |
| AlwNI | CAGNNNCTG | 4 cut(s) | 494, 545, 1472, 1607 |
| Ama87I | CYCGRG | 1 cut(s) | 2741 |
| ApoI | RAATTY | 4 cut(s) | 2067, 2386, 2393, 2852 |
| AseI | ATTAAT | 1 cut(s) | 309 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 2386, 3053 |
| AspA2I | CCTAGG | 1 cut(s) | 1561 |
| AspLEI | GCGC | 6 cut(s) | 739, 1358, 1432, 1531, 2564, 2723 |
| AsuC2I | CCSGG | 3 cut(s) | 373, 1943, 2725 |
| AsuHPI | GGTGA | 5 cut(s) | 773, 1151, 1550, 2186, 2516 |
| AvaI | CYCGRG | 1 cut(s) | 2741 |
| AvaII | GGWCC | 9 cut(s) | 362, 383, 497, 596, 977, 1939, 2356, 2897, 2996 |
| AvrII | CCTAGG | 1 cut(s) | 1561 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 573, 606 |
| BalI | TGGCCA | 2 cut(s) | 1020, 1057 |
| BanI | GGYRCC | 3 cut(s) | 83, 2561, 2720 |
| BanII | GRGCYC | 1 cut(s) | 394 |
| BbvCI | CCTCAGC | 2 cut(s) | 429, 570 |
| BceAI | ACGGC | 1 cut(s) | 1275 |
| BciVI | GTATCC | 1 cut(s) | 2951 |
| BclI | TGATCA | 1 cut(s) | 1213 |
| BcnI | CCSGG | 3 cut(s) | 373, 1943, 2725 |
| BcuI | ACTAGT | 1 cut(s) | 584 |
| BfaI | CTAG | 8 cut(s) | 585, 1314, 1562, 2322, 2340, 2661, 2805, 3058 |
| BfmI | CTRYAG | 3 cut(s) | 279, 887, 960 |
| BfoI | RGCGCY | 4 cut(s) | 740, 1532, 2565, 2724 |
| BfuAI | ACCTGC | 2 cut(s) | 118, 1701 |
| BfuI | GTATCC | 1 cut(s) | 2951 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1054 |
| BglII | AGATCT | 3 cut(s) | 238, 1734, 1963 |
| BlnI | CCTAGG | 1 cut(s) | 1561 |
| BlpI | GCTNAGC | 1 cut(s) | 685 |
| BmcAI | AGTACT | 1 cut(s) | 895 |
| Bme18I | GGWCC | 9 cut(s) | 362, 383, 497, 596, 977, 1939, 2356, 2897, 2996 |
| BmeT110I | CYCGRG | 1 cut(s) | 2741 |
| BmiI | GGNNCC | 9 cut(s) | 85, 393, 1853, 2358, 2563, 2722, 2899, 2989, 2998 |
| BmrI | ACTGGG | 1 cut(s) | 1356 |
| BmsI | GCATC | 9 cut(s) | 571, 1329, 1531, 1988, 2455, 2477, 2641, 2663, 2962 |
| BmuI | ACTGGG | 1 cut(s) | 1356 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 2009, 2041, 2150, 2182, 2737, 2769 |
| BpmI | CTGGAG | 6 cut(s) | 657, 1189, 1636, 2349, 2671, 2862 |
| Bpu10I | CCTNAGC | 2 cut(s) | 429, 570 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 685 |
| BpuEI | CTTGAG | 2 cut(s) | 1988, 2602 |
| BpuMI | CCSGG | 3 cut(s) | 373, 1943, 2725 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1390 |
| BsaHI | GRCGYC | 3 cut(s) | 2562, 2721, 2835 |
| BsaI | GGTCTC | 3 cut(s) | 442, 1453, 1934 |
| BsaWI | WCCGGW | 2 cut(s) | 986, 1674 |
| BsaXI | ACNNNNNCTCC | 3 cut(s) | 550, 580, 3035 |
| Bse3DI | GCAATG | 1 cut(s) | 2137 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1390 |
| BseJI | GATNNNNATC | 1 cut(s) | 1390 |
| BseMI | GCAATG | 1 cut(s) | 2137 |
| BseRI | GAGGAG | 2 cut(s) | 558, 1808 |
| BseYI | CCCAGC | 1 cut(s) | 2603 |
| BsgI | GTGCAG | 4 cut(s) | 312, 1026, 1458, 2100 |
| Bsh1236I | CGCG | 1 cut(s) | 1432 |
| Bsh1285I | CGRYCG | 2 cut(s) | 360, 1305 |
| BshNI | GGYRCC | 3 cut(s) | 83, 2561, 2720 |
| BsiEI | CGRYCG | 2 cut(s) | 360, 1305 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2741 |
| BsiSI | CCGG | 5 cut(s) | 372, 987, 1675, 1942, 2724 |
| BslFI | GGGAC | 6 cut(s) | 818, 2308, 2342, 2585, 2883, 2982 |
| BsmBI | CGTCTC | 1 cut(s) | 2826 |
| BsmFI | GGGAC | 6 cut(s) | 818, 2308, 2342, 2585, 2883, 2982 |
| BsmI | GAATGC | 2 cut(s) | 1642, 2350 |
| Bso31I | GGTCTC | 3 cut(s) | 442, 1453, 1934 |
| BsoBI | CYCGRG | 1 cut(s) | 2741 |
| Bsp1286I | GDGCHC | 1 cut(s) | 394 |
| Bsp1407I | TGTACA | 2 cut(s) | 1408, 1701 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 685 |
| Bsp19I | CCATGG | 1 cut(s) | 2953 |
| BspACI | CCGC | 8 cut(s) | 5, 491, 654, 1276, 1305, 1432, 1870, 2272 |
| BspFNI | CGCG | 1 cut(s) | 1432 |
| BspLI | GGNNCC | 9 cut(s) | 85, 393, 1853, 2358, 2563, 2722, 2899, 2989, 2998 |
| BspMAI | CTGCAG | 2 cut(s) | 283, 964 |
| BspMI | ACCTGC | 2 cut(s) | 118, 1701 |
| BspPI | GGATC | 7 cut(s) | 33, 83, 118, 722, 1021, 1399, 2506 |
| BspT107I | GGYRCC | 3 cut(s) | 83, 2561, 2720 |
| BspTNI | GGTCTC | 3 cut(s) | 442, 1453, 1934 |
| BsrBI | CCGCTC | 1 cut(s) | 1276 |
| BsrDI | GCAATG | 1 cut(s) | 2137 |
| BsrGI | TGTACA | 2 cut(s) | 1408, 1701 |
| BssNAI | GTATAC | 1 cut(s) | 1502 |
| BssNI | GRCGYC | 3 cut(s) | 2562, 2721, 2835 |
| BssT1I | CCWWGG | 4 cut(s) | 935, 1517, 1561, 2953 |
| Bst1107I | GTATAC | 1 cut(s) | 1502 |
| Bst4CI | ACNGT | 9 cut(s) | 170, 220, 358, 2194, 2306, 2330, 2751, 2825, 2879 |
| Bst6I | CTCTTC | 2 cut(s) | 1836, 3016 |
| BstACI | GRCGYC | 3 cut(s) | 2562, 2721, 2835 |
| BstAUI | TGTACA | 2 cut(s) | 1408, 1701 |
| BstC8I | GCNNGC | 8 cut(s) | 942, 949, 960, 1146, 1582, 2053, 2057, 2719 |
| BstDSI | CCRYGG | 2 cut(s) | 1052, 2953 |
| BstEII | GGTNACC | 1 cut(s) | 1173 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1476, 2803 |
| BstFNI | CGCG | 1 cut(s) | 1432 |
| BstH2I | RGCGCY | 4 cut(s) | 740, 1532, 2565, 2724 |
| BstHHI | GCGC | 6 cut(s) | 739, 1358, 1432, 1531, 2564, 2723 |
| BstMCI | CGRYCG | 2 cut(s) | 360, 1305 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 326, 398, 439, 931, 937, 1054, 1550 |
| BstNSI | RCATGY | 2 cut(s) | 769, 1199 |
| BstPI | GGTNACC | 1 cut(s) | 1173 |
| BstSFI | CTRYAG | 3 cut(s) | 279, 887, 960 |
| BstUI | CGCG | 1 cut(s) | 1432 |
| BstX2I | RGATCY | 6 cut(s) | 110, 238, 1013, 1391, 1734, 1963 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2055 |
| BstYI | RGATCY | 6 cut(s) | 110, 238, 1013, 1391, 1734, 1963 |
| BstZ17I | GTATAC | 1 cut(s) | 1502 |
| BsuI | GTATCC | 1 cut(s) | 2951 |
| BtgI | CCRYGG | 2 cut(s) | 1052, 2953 |
| BtgZI | GCGATG | 1 cut(s) | 2664 |
| BtsI | GCAGTG | 3 cut(s) | 969, 1337, 1501 |
| BveI | ACCTGC | 2 cut(s) | 118, 1701 |
| Cac8I | GCNNGC | 8 cut(s) | 942, 949, 960, 1146, 1582, 2053, 2057, 2719 |
| CaiI | CAGNNNCTG | 4 cut(s) | 494, 545, 1472, 1607 |
| CfoI | GCGC | 6 cut(s) | 739, 1358, 1432, 1531, 2564, 2723 |
| Csp6I | GTAC | 5 cut(s) | 894, 1409, 1702, 2190, 2931 |
| CviAII | CATG | 9 cut(s) | 22, 766, 1196, 1324, 1367, 1903, 2231, 2279, 2954 |
| CviQI | GTAC | 5 cut(s) | 894, 1409, 1702, 2190, 2931 |
| DinI | GGCGCC | 2 cut(s) | 2563, 2722 |
| DraI | TTTAAA | 1 cut(s) | 3004 |
| DraIII | CACNNNGTG | 1 cut(s) | 170 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2333 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2333 |
| EaeI | YGGCCR | 3 cut(s) | 1018, 1055, 1433 |
| Eam1104I | CTCTTC | 2 cut(s) | 1836, 3016 |
| EarI | CTCTTC | 2 cut(s) | 1836, 3016 |
| EciI | GGCGGA | 1 cut(s) | 1885 |
| Eco130I | CCWWGG | 4 cut(s) | 935, 1517, 1561, 2953 |
| Eco147I | AGGCCT | 1 cut(s) | 38 |
| Eco24I | GRGCYC | 1 cut(s) | 394 |
| Eco31I | GGTCTC | 3 cut(s) | 442, 1453, 1934 |
| Eco47I | GGWCC | 9 cut(s) | 362, 383, 497, 596, 977, 1939, 2356, 2897, 2996 |
| Eco57I | CTGAAG | 3 cut(s) | 897, 911, 1919 |
| Eco88I | CYCGRG | 1 cut(s) | 2741 |
| Eco91I | GGTNACC | 1 cut(s) | 1173 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1476, 2803 |
| EcoO109I | RGGNCCY | 4 cut(s) | 1481, 2032, 2356, 2996 |
| EcoO65I | GGTNACC | 1 cut(s) | 1173 |
| EcoRI | GAATTC | 3 cut(s) | 2067, 2386, 2393 |
| EcoT14I | CCWWGG | 4 cut(s) | 935, 1517, 1561, 2953 |
| EcoT22I | ATGCAT | 2 cut(s) | 2470, 2656 |
| EcoT38I | GRGCYC | 1 cut(s) | 394 |
| EgeI | GGCGCC | 2 cut(s) | 2563, 2722 |
| EheI | GGCGCC | 2 cut(s) | 2563, 2722 |
| ErhI | CCWWGG | 4 cut(s) | 935, 1517, 1561, 2953 |
| Esp3I | CGTCTC | 1 cut(s) | 2826 |
| FaeI | CATG | 9 cut(s) | 25, 769, 1199, 1327, 1370, 1906, 2234, 2282, 2957 |
| FaqI | GGGAC | 6 cut(s) | 818, 2308, 2342, 2585, 2883, 2982 |
| FatI | CATG | 9 cut(s) | 21, 765, 1195, 1323, 1366, 1902, 2230, 2278, 2953 |
| FbaI | TGATCA | 1 cut(s) | 1213 |
| FblI | GTMKAC | 2 cut(s) | 1501, 2040 |
| FriOI | GRGCYC | 1 cut(s) | 394 |
| FspBI | CTAG | 8 cut(s) | 585, 1314, 1562, 2322, 2340, 2661, 2805, 3058 |
| GlaI | GCGC | 6 cut(s) | 738, 1357, 1431, 1530, 2563, 2722 |
| GsaI | CCCAGC | 1 cut(s) | 2607 |
| GsuI | CTGGAG | 6 cut(s) | 657, 1189, 1636, 2349, 2671, 2862 |
| HaeII | RGCGCY | 4 cut(s) | 740, 1532, 2565, 2724 |
| HapII | CCGG | 5 cut(s) | 372, 987, 1675, 1942, 2724 |
| HhaI | GCGC | 6 cut(s) | 739, 1358, 1432, 1531, 2564, 2723 |
| Hin1I | GRCGYC | 3 cut(s) | 2562, 2721, 2835 |
| Hin1II | CATG | 9 cut(s) | 25, 769, 1199, 1327, 1370, 1906, 2234, 2282, 2957 |
| Hin6I | GCGC | 6 cut(s) | 737, 1356, 1430, 1529, 2562, 2721 |
| HinP1I | GCGC | 6 cut(s) | 737, 1356, 1430, 1529, 2562, 2721 |
| HincII | GTYRAC | 1 cut(s) | 2023 |
| HindII | GTYRAC | 1 cut(s) | 2023 |
| HindIII | AAGCTT | 1 cut(s) | 2554 |
| HpaI | GTTAAC | 1 cut(s) | 2023 |
| HpaII | CCGG | 5 cut(s) | 372, 987, 1675, 1942, 2724 |
| HphI | GGTGA | 5 cut(s) | 773, 1151, 1550, 2186, 2516 |
| Hpy166II | GTNNAC | 4 cut(s) | 1502, 1900, 2023, 2041 |
| Hpy8I | GTNNAC | 4 cut(s) | 1502, 1900, 2023, 2041 |
| HpyCH4III | ACNGT | 9 cut(s) | 170, 220, 358, 2194, 2306, 2330, 2751, 2825, 2879 |
| HpyCH4IV | ACGT | 2 cut(s) | 611, 2835 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 326, 398, 439, 931, 937, 1054, 1550 |
| HpySE526I | ACGT | 2 cut(s) | 611, 2835 |
| Hsp92I | GRCGYC | 3 cut(s) | 2562, 2721, 2835 |
| Hsp92II | CATG | 9 cut(s) | 25, 769, 1199, 1327, 1370, 1906, 2234, 2282, 2957 |
| HspAI | GCGC | 6 cut(s) | 737, 1356, 1430, 1529, 2562, 2721 |
| KasI | GGCGCC | 2 cut(s) | 2561, 2720 |
| Ksp22I | TGATCA | 1 cut(s) | 1213 |
| KspAI | GTTAAC | 1 cut(s) | 2023 |
| LmnI | GCTCC | 8 cut(s) | 397, 638, 1273, 1549, 1906, 2780, 2792, 2843 |
| LweI | GCATC | 9 cut(s) | 571, 1329, 1531, 1988, 2455, 2477, 2641, 2663, 2962 |
| MaeI | CTAG | 8 cut(s) | 585, 1314, 1562, 2322, 2340, 2661, 2805, 3058 |
| MaeII | ACGT | 2 cut(s) | 611, 2835 |
| MbiI | CCGCTC | 1 cut(s) | 1276 |
| MboII | GAAGA | 8 cut(s) | 804, 1583, 1853, 1885, 2126, 2321, 2724, 3003 |
| MfeI | CAATTG | 1 cut(s) | 1893 |
| MflI | RGATCY | 6 cut(s) | 110, 238, 1013, 1391, 1734, 1963 |
| MhlI | GDGCHC | 1 cut(s) | 394 |
| MlsI | TGGCCA | 2 cut(s) | 1020, 1057 |
| MluNI | TGGCCA | 2 cut(s) | 1020, 1057 |
| Mly113I | GGCGCC | 2 cut(s) | 2562, 2721 |
| MlyI | GAGTC | 6 cut(s) | 725, 1643, 1894, 2399, 2690, 2912 |
| MmeI | TCCRAC | 8 cut(s) | 53, 76, 559, 877, 884, 991, 1469, 1995 |
| Mox20I | TGGCCA | 2 cut(s) | 1020, 1057 |
| Mph1103I | ATGCAT | 2 cut(s) | 2470, 2656 |
| MroXI | GAANNNNTTC | 2 cut(s) | 2386, 3053 |
| MscI | TGGCCA | 2 cut(s) | 1020, 1057 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1371, 2283 |
| Msp20I | TGGCCA | 2 cut(s) | 1020, 1057 |
| MspA1I | CMGCKG | 4 cut(s) | 491, 654, 1607, 2051 |
| MspI | CCGG | 5 cut(s) | 372, 987, 1675, 1942, 2724 |
| MunI | CAATTG | 1 cut(s) | 1893 |
| Mva1269I | GAATGC | 2 cut(s) | 1642, 2350 |
| MvnI | CGCG | 1 cut(s) | 1432 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 326, 398, 439, 931, 937, 1054, 1550 |
| NarI | GGCGCC | 2 cut(s) | 2562, 2721 |
| NciI | CCSGG | 3 cut(s) | 373, 1943, 2725 |
| NcoI | CCATGG | 1 cut(s) | 2953 |
| NlaIII | CATG | 9 cut(s) | 25, 769, 1199, 1327, 1370, 1906, 2234, 2282, 2957 |
| NlaIV | GGNNCC | 9 cut(s) | 85, 393, 1853, 2358, 2563, 2722, 2899, 2989, 2998 |
| NmuCI | GTSAC | 7 cut(s) | 162, 717, 761, 1115, 1715, 2504, 2836 |
| NsiI | ATGCAT | 2 cut(s) | 2470, 2656 |
| NspI | RCATGY | 2 cut(s) | 769, 1199 |
| PaqCI | CACCTGC | 1 cut(s) | 1701 |
| PasI | CCCWGGG | 1 cut(s) | 1179 |
| PceI | AGGCCT | 1 cut(s) | 38 |
| PciI | ACATGT | 1 cut(s) | 765 |
| PctI | GAATGC | 2 cut(s) | 1642, 2350 |
| PdmI | GAANNNNTTC | 2 cut(s) | 2386, 3053 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 494, 1451 |
| PleI | GAGTC | 6 cut(s) | 724, 1643, 1893, 2399, 2690, 2912 |
| PluTI | GGCGCC | 2 cut(s) | 2565, 2724 |
| PpsI | GAGTC | 6 cut(s) | 724, 1643, 1893, 2399, 2690, 2912 |
| PpuMI | RGGWCCY | 2 cut(s) | 2356, 2996 |
| PscI | ACATGT | 1 cut(s) | 765 |
| PshBI | ATTAAT | 1 cut(s) | 309 |
| PsiI | TTATAA | 1 cut(s) | 198 |
| Psp1406I | AACGTT | 1 cut(s) | 611 |
| Psp5II | RGGWCCY | 2 cut(s) | 2356, 2996 |
| PspEI | GGTNACC | 1 cut(s) | 1173 |
| PspFI | CCCAGC | 1 cut(s) | 2603 |
| PspN4I | GGNNCC | 9 cut(s) | 85, 393, 1853, 2358, 2563, 2722, 2899, 2989, 2998 |
| PspPPI | RGGWCCY | 2 cut(s) | 2356, 2996 |
| PstI | CTGCAG | 2 cut(s) | 283, 964 |
| PstNI | CAGNNNCTG | 4 cut(s) | 494, 545, 1472, 1607 |
| PsuI | RGATCY | 6 cut(s) | 110, 238, 1013, 1391, 1734, 1963 |
| PvuII | CAGCTG | 2 cut(s) | 1607, 2051 |
| RsaI | GTAC | 5 cut(s) | 895, 1410, 1703, 2191, 2932 |
| RsaNI | GTAC | 5 cut(s) | 894, 1409, 1702, 2190, 2931 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1371, 2283 |
| ScaI | AGTACT | 1 cut(s) | 895 |
| SchI | GAGTC | 6 cut(s) | 725, 1643, 1894, 2399, 2690, 2912 |
| SduI | GDGCHC | 1 cut(s) | 394 |
| SfaNI | GCATC | 9 cut(s) | 571, 1329, 1531, 1988, 2455, 2477, 2641, 2663, 2962 |
| SfcI | CTRYAG | 3 cut(s) | 279, 887, 960 |
| SfoI | GGCGCC | 2 cut(s) | 2563, 2722 |
| SinI | GGWCC | 9 cut(s) | 362, 383, 497, 596, 977, 1939, 2356, 2897, 2996 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1371, 2283 |
| SmlI | CTYRAG | 2 cut(s) | 1967, 2617 |
| SmoI | CTYRAG | 2 cut(s) | 1967, 2617 |
| SpeI | ACTAGT | 1 cut(s) | 584 |
| SseBI | AGGCCT | 1 cut(s) | 38 |
| SsiI | CCGC | 8 cut(s) | 5, 491, 654, 1276, 1305, 1432, 1870, 2272 |
| SspDI | GGCGCC | 2 cut(s) | 2561, 2720 |
| SspMI | CTAG | 8 cut(s) | 585, 1314, 1562, 2322, 2340, 2661, 2805, 3058 |
| StuI | AGGCCT | 1 cut(s) | 38 |
| StyI | CCWWGG | 4 cut(s) | 935, 1517, 1561, 2953 |
| TaaI | ACNGT | 9 cut(s) | 170, 220, 358, 2194, 2306, 2330, 2751, 2825, 2879 |
| TaiI | ACGT | 2 cut(s) | 614, 2838 |
| TaqI | TCGA | 8 cut(s) | 33, 879, 2027, 2408, 2694, 2811, 2858, 2916 |
| TaqII | GACCGA | 2 cut(s) | 150, 350 |
| TatI | WGTACW | 4 cut(s) | 893, 1408, 1701, 2930 |
| TauI | GCSGC | 2 cut(s) | 494, 1435 |
| TseFI | GTSAC | 7 cut(s) | 162, 717, 761, 1115, 1715, 2504, 2836 |
| Tsp45I | GTSAC | 7 cut(s) | 162, 717, 761, 1115, 1715, 2504, 2836 |
| TspDTI | ATGAA | 8 cut(s) | 1079, 1248, 1347, 1641, 1994, 2133, 2939, 3033 |
| TspGWI | ACGGA | 3 cut(s) | 78, 1041, 1844 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 494, 1451 |
| VpaK11BI | GGWCC | 9 cut(s) | 362, 383, 497, 596, 977, 1939, 2356, 2897, 2996 |
| VspI | ATTAAT | 1 cut(s) | 309 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1476, 2803 |
| XapI | RAATTY | 4 cut(s) | 2067, 2386, 2393, 2852 |
| XceI | RCATGY | 2 cut(s) | 769, 1199 |
| XmaJI | CCTAGG | 1 cut(s) | 1561 |
| XmiI | GTMKAC | 2 cut(s) | 1501, 2040 |
| XmnI | GAANNNNTTC | 2 cut(s) | 2386, 3053 |
| XspI | CTAG | 8 cut(s) | 585, 1314, 1562, 2322, 2340, 2661, 2805, 3058 |
| ZraI | GACGTC | 1 cut(s) | 2836 |
| ZrmI | AGTACT | 1 cut(s) | 895 |
| Zsp2I | ATGCAT | 2 cut(s) | 2470, 2656 |