Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7B

Orthogroup ID

OG0007032

Physical Location & Seq

Genomic Coordinates Forward (+)

26889600 .. 26898558

Gene Structure 8959 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7BG286300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2916 bp

ATGCTGCTCAGAACTGCTTTGATATGGATGCATGCGGATCAGAACAAAACTGGTTTCCTTGGTCGGCCAGAATTTTATAATGCTCTTAGACTTGTGACTGTGGCTCAAAGCAAGAGAGATTTAACGCCTGATATAGTCAAGGCCGCATTATACGGGCCAGCTACTGCAAAAATTCCTGCTCCACAAATCAATCTTTCAGCCATATCTGCACCTCAAGCAAATCCTATGGCTGGTGCACCTGCTCCACAGATGGGTATGGGGATGCCACCAACATCTCAAAGTTTTGGGTTTAGAGGACCAGGAGCTCCTAATGCAGGTATGAACCAGAATTATTTTCAGCCACAGCAAAACCAGTCCATGAGACCTCCTCAAGGCATGACCCCTGGGATGCCTACTAGTGGATATTCCCGTCCACAACAGGGTGTGGGAGGTGGTATGGGGGGTCCCAATGTAATGAACTCAAATAATTGGCTTAGTGGAAGCACTGGTGCACCTCCTTCTGGTCCGAGAGGGATTAGTCCTTCCATGCCCTCATCTACAACACAACCACAACCTCCAGTGTCAACATCTTCCCTGGCGACAGTGAATGACTCTAAATCATTAGTTCCTTCTGGAAATGGGTTTGCATCCAATTCAGGTTTCTCAGGTGATTTATTTTCTGCAGCCCCTGCTCAATCAAAGCCAGGAGCTCCTGGGTCAACATATTCTGCTAGTAGTGCACCTACGCCATCAGCCATTGTTCCAGTTTCAAGTGGGTCCCAATCTTCTAGTAAGCTCAATGCACTTGATTCACTGAGTGCATTCACAATGCAGCCTTCTGGTGGCCAATTTCAGCGGCCCCAGGCACCGTCAAACTCAAGCCCGCAAGTTTCAGCTGCGGGTCCTTCCTCTTTTGCATCACCCGCAATTTCAGTTGGAGTCGGAAACTCTACTTCTGAAAATTCACAGCCTCCTTGGCCAAAGATGAAGCCATCTGATGTTCAGAAATATACAAAAGTCTTTATGGAAGTAGACACTGACAGAGATGGGAAAATCACTGGAGAGCAGGCACGAAATCTATTTCTGAGCTGGAGGTTACCAAGAGAGGTTCTAAAGCAGGTGTGGGACTTATCTGATCAGGATAATGACAGCATGCTTTCCTTGAGAGAGTTCTGCTTTTCTCTCTATTTGATGGAACGTTACAGGGAAGGTCGGCCTCTTCCAGCTACACTTCCAAGCAATGTTATGTTGGATGAGACACTGCTGTCAATGACAGGTCAACCAAAAGTGGGTTATGGAAATGCAGCTTGGAGTCCCCACCCTGGCTTTGGACAACATCAAGGGATGCAAGGTTCCCAGATGATGCCACCTGGCACTGGTTTGAGGCCACCAATTCAGGGAAACGCCCCTCAGGCTGATCGTACAGTGCAACCGAATCAGCAAAATTTGAGGGTGCCAGGGATGGAGGGCTTGGCTCAAAACCAGCTTGACAATGGGAAGCAGGATTCAGCCAACTCAAGACCTCAAGACCCTAACAGTGCAGGGAAAGAGGTTGAAGAAACTGAGAATGTGATTTTGGACTCAAGAGAGAAGATTGAGTTCTATCGCACAAAAATGCAGGAACTTGTTCTGTATAAAAGCAGATGTGATAATAGATTAAATGAGATCACAGAAAGGGCACTGGCAGACAAGCGTGAGGCTGAACTACTGGGTAAGAAGTATGAAGAGAAATATAAACAAGTGGCAGAAATAGCATCTAAGTTAACCATTGAGGAGGCCACGTTTCGTGAAGTTCAGGAGAGGAAGATGGAATTGCATCAAGCAATTGTTAGAATGGAGCAAGGAGGTAGTGCTGATGGTATCCTTCAGGTCCGAGCTGATCGTATACAATATGATCTTGAGGAGCTAATAAAGGCTCTTACTGAACGTTGCAAGAAACATGGGCTAGACATGAAGTCAGCTGCAATAATTGAGCTTCCAACTGGTTGGCAACCTGGAATTCAAGACGGAGCAGCTGTTTGGGATGAAGATTGGGACAAGTTCGAAGATGAAGGATTTGGCAATGATCTCATAATTGATTCCTCAACAAAATCTGTCCAGAGAGAAAAGGCTTCCCCAGATCGTAGTTCAACTGCTGATTCATCATCGGTTACTGATGGAAAGTCAGGAATTTCTTACAGCAATGGTGATCATGCACATGAAAGTGAATCTGTTTTCACCCACGGTGAAGATGAACATGTAAGAAGTCCCAATGGCAGTCTAGCTGGAAGGACTGCTGTAGACAGCCCGTCTCGAGACTTCTCAGACATCCACTATGGTAAAAATTCCGAAGCAGAAGGAGAAACACACGGAAGTTTTGATGAATCGACCTGGGGTGCCTTTGACAATAATGATGATATTGATTCCGTTTGGGGATTTAATACTGACAAGGGCAAGGGCACAGATTCTGAGAAGCACAGAGATTTCTTTGGATCTGATGACTATGGTGTCAACCCAGTAAGAACTGGATCCCCCAATGCAGATACCGCCTTTCAGAAGAAGAGCATTTTCTTTGAAGACTCTGTTCCTAGCACACCAGTCTCGAGGTTTGCCAACTCGCCAAGATATAGTGAGGCCGGGGATCACTACTTTGACAATTTCTCAAGGTTTGATTCATTCAGCAGCAGGCAAGACAGTGGTTTTTCTTCTCAACCTGAGAGGTTCACAAGGTTTGATTCCATGAATAGCACTAGAGATTTTGGCCACTCGAGGTTTGATTCCGTAAGCAGCAGCAAAGACTTTGGCCAAAGTCATGGCCACACAAGGTTCGATTCCATAAACAGCACAAAAGATTTTGGTTTCGATGACACAGATCCATTTGGCTCTTCTGGCCCCTTTAAGGTCACATCAGAGAGTCAAAATCCAAAGAAGGGATCTGATAATTGGAATGCTTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000147 GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005938 GO:0006810 GO:0006886 GO:0006897 GO:0006996 GO:0007010 GO:0008104 GO:0008150 GO:0009987 GO:0015031 GO:0015629 GO:0015833 GO:0016043 GO:0016192 GO:0022607 GO:0030029 GO:0030036 GO:0030479 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0033036 GO:0034613 GO:0042886 GO:0043167 GO:0043169 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044396 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045184 GO:0046872 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061645 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0098657 GO:0099568

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

EF-hand_4 EF-hand_5

Protein Analysis

971

Amino Acids

105.41

Weight (kDa)

5.27

Isoelectric Point (pI)

50.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EH	PF12763	323 - 405	3.8e-12	EH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007032)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20760
fragaria_vesca	FvH4_5g25580 FvH4_5g25580
malus_domestica	MD06G1007400.v1.1
prunus_persica	Prupe.5G009300_v2.0.a1 Prupe.5G009300_v2.0.a1
pyrus_communis	pycom06g00650 pycom06g00660
rosa_chinensis	RchiOBHm_Chr7g0215891 RchiOBHm_Chr7g0219801 RchiOBHm_Chr7g0219941
rosa_laevigata	RLG00000002612
rosa_multiflora	Rmu_co8383945.1_g000001 Rmu_sc0006240.1_g000011
rosa_roxburghii	Rroxscaffold_3G00243830
rosa_rugosa	Rorug07G0156400 Rorug07G0156500
rosa_samantha	Rh7AG294500 Rh7AG298200 Rh7BG286300 Rh7CG314500 Rh7CG318200 Rh7CG318300 Rh7DG297400
rosa_wichuraiana	Rw7G025020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	78
AarI	CACCTGC	2 cut(s)	247, 1087
AasI	GACNNNNNNGTC	2 cut(s)	1243, 2465
Acc36I	ACCTGC	3 cut(s)	247, 305, 1087
AccB1I	GGYRCC	3 cut(s)	844, 1432, 2354
AccI	GTMKAC	3 cut(s)	1011, 1864, 2259
AciI	CCGC	7 cut(s)	35, 144, 835, 863, 878, 903, 2505
AclI	AACGTT	2 cut(s)	1177, 1906
AclWI	GGATC	7 cut(s)	45, 2458, 2481, 2494, 2607, 2825, 2899
AcoI	YGGCCR	6 cut(s)	65, 823, 956, 2719, 2761, 2773
AcsI	RAATTY	7 cut(s)	71, 171, 940, 1423, 1977, 2148, 2302
AcuI	CTGAAG	1 cut(s)	1829
AdeI	CACNNNGTG	1 cut(s)	797
AfaI	GTAC	1 cut(s)	1402
AflIII	ACRYGT	1 cut(s)	2215
AgsI	TTSAA	5 cut(s)	750, 1535, 1982, 2109, 2534
AhdI	GACNNNNNGTC	2 cut(s)	1933, 2266
AhlI	ACTAGT	1 cut(s)	395
AjuI	GAANNNNNNNTTGG	6 cut(s)	623, 655, 1270, 1302, 1538, 1570
AloI	GAACNNNNNNTCC	2 cut(s)	1315, 1347
Alw21I	GWGCWC	5 cut(s)	238, 307, 493, 691, 721
Alw26I	GTCTC	5 cut(s)	355, 1229, 2268, 2274, 2563
Alw44I	GTGCAC	3 cut(s)	234, 489, 717
AlwI	GGATC	7 cut(s)	45, 2458, 2481, 2494, 2607, 2825, 2899
AlwNI	CAGNNNCTG	3 cut(s)	164, 668, 2426
Ama87I	CYCGRG	3 cut(s)	2271, 2560, 2725
ApaLI	GTGCAC	3 cut(s)	234, 489, 717
ApoI	RAATTY	7 cut(s)	71, 171, 940, 1423, 1977, 2148, 2302
Asp700I	GAANNNNTTC	2 cut(s)	1605, 2909
AspS9I	GGNCC	9 cut(s)	155, 296, 443, 503, 756, 837, 881, 1849, 2849
AsuC2I	CCSGG	1 cut(s)	2596
AsuHPI	GGTGA	5 cut(s)	659, 891, 2177, 2188, 2216
AsuII	TTCGAA	1 cut(s)	2022
AvaI	CYCGRG	3 cut(s)	2271, 2560, 2725
AvaII	GGWCC	6 cut(s)	296, 443, 503, 756, 881, 1849
AxyI	CCTNAGG	1 cut(s)	1389
BaeGI	GKGCMC	5 cut(s)	238, 493, 721, 1660, 2420
BalI	TGGCCA	5 cut(s)	825, 958, 2721, 2763, 2775
BamHI	GGATCC	1 cut(s)	2486
BanI	GGYRCC	3 cut(s)	844, 1432, 2354
BanII	GRGCYC	2 cut(s)	307, 691
BbsI	GAAGAC	1 cut(s)	2541
Bbv12I	GWGCWC	5 cut(s)	238, 307, 493, 691, 721
BbvI	GCAGC	9 cut(s)	674, 823, 862, 1295, 1927, 2003, 2652, 2757, 2760
BccI	CCATC	9 cut(s)	244, 736, 979, 1019, 1165, 1435, 1780, 1829, 2129
BciVI	GTATCC	1 cut(s)	1850
BclI	TGATCA	2 cut(s)	1114, 2167
BcnI	CCSGG	1 cut(s)	2596
BcoDI	GTCTC	5 cut(s)	355, 1229, 2268, 2274, 2563
BcuI	ACTAGT	1 cut(s)	395
BfaI	CTAG	7 cut(s)	396, 711, 768, 1925, 2240, 2547, 2709
BfmI	CTRYAG	2 cut(s)	660, 2256
BfuAI	ACCTGC	3 cut(s)	247, 305, 1087
BfuI	GTATCC	1 cut(s)	1850
BglI	GCCNNNNNGGC	2 cut(s)	955, 1391
Bme18I	GGWCC	6 cut(s)	296, 443, 503, 756, 881, 1849
BmeRI	GACNNNNNGTC	2 cut(s)	1933, 2266
BmeT110I	CYCGRG	3 cut(s)	2271, 2560, 2725
BmgT120I	GGNCC	9 cut(s)	155, 296, 443, 503, 756, 837, 881, 1849, 2849
BmrI	ACTGGG	2 cut(s)	1697, 2468
BmsI	GCATC	9 cut(s)	18, 252, 378, 635, 905, 1314, 1332, 1742, 1804
BmuI	ACTGGG	2 cut(s)	1697, 2468
BpiI	GAAGAC	1 cut(s)	2541
BplI	GAGNNNNNCTC	2 cut(s)	352, 384
BpmI	CTGGAG	3 cut(s)	540, 1059, 1090
Bpu14I	TTCGAA	1 cut(s)	2022
BpuEI	CTTGAG	9 cut(s)	198, 354, 841, 1162, 1480, 1488, 1546, 1898, 2605
BpuMI	CCSGG	1 cut(s)	2596
BsaI	GGTCTC	1 cut(s)	355
BsaXI	ACNNNNNCTCC	4 cut(s)	1745, 1775, 1980, 2010
Bse21I	CCTNAGG	1 cut(s)	1389
Bse3DI	GCAATG	3 cut(s)	1225, 2047, 2167
BseGI	GGATG	9 cut(s)	33, 267, 393, 626, 1237, 1329, 1446, 2008, 2286
BseMI	GCAATG	3 cut(s)	1225, 2047, 2167
BseMII	CTCAG	9 cut(s)	22, 657, 785, 1055, 1403, 1533, 2295, 2418, 2664
BseRI	GAGGAG	3 cut(s)	357, 1766, 1895
BseSI	GKGCMC	5 cut(s)	238, 493, 721, 1660, 2420
BseXI	GCAGC	9 cut(s)	674, 823, 862, 1295, 1927, 2003, 2652, 2757, 2760
BsgI	GTGCAG	2 cut(s)	192, 1539
BshNI	GGYRCC	3 cut(s)	844, 1432, 2354
BsiHKAI	GWGCWC	5 cut(s)	238, 307, 493, 691, 721
BsiHKCI	CYCGRG	3 cut(s)	2271, 2560, 2725
BsiSI	CCGG	1 cut(s)	2595
BslFI	GGGAC	6 cut(s)	429, 742, 1118, 1278, 2027, 2211
BsmAI	GTCTC	5 cut(s)	355, 1229, 2268, 2274, 2563
BsmBI	CGTCTC	1 cut(s)	2274
BsmFI	GGGAC	6 cut(s)	429, 742, 1118, 1278, 2027, 2211
BsmI	GAATGC	2 cut(s)	800, 2911
Bso31I	GGTCTC	1 cut(s)	355
BsoBI	CYCGRG	3 cut(s)	2271, 2560, 2725
Bsp119I	TTCGAA	1 cut(s)	2022
Bsp1286I	GDGCHC	7 cut(s)	238, 307, 493, 691, 721, 1660, 2420
BspACI	CCGC	7 cut(s)	35, 144, 835, 863, 878, 903, 2505
BspCNI	CTCAG	9 cut(s)	21, 656, 786, 1056, 1402, 1534, 2294, 2419, 2665
BspMAI	CTGCAG	1 cut(s)	664
BspMI	ACCTGC	3 cut(s)	247, 305, 1087
BspPI	GGATC	7 cut(s)	45, 2458, 2481, 2494, 2607, 2825, 2899
BspQI	GCTCTTC	2 cut(s)	2513, 2848
BspT104I	TTCGAA	1 cut(s)	2022
BspT107I	GGYRCC	3 cut(s)	844, 1432, 2354
BspTNI	GGTCTC	1 cut(s)	355
BsrDI	GCAATG	3 cut(s)	1225, 2047, 2167
BssNAI	GTATAC	1 cut(s)	1865
BssT1I	CCWWGG	2 cut(s)	58, 953
Bst1107I	GTATAC	1 cut(s)	1865
Bst4CI	ACNGT	7 cut(s)	100, 583, 849, 1405, 1517, 2204, 2654
Bst6I	CTCTTC	4 cut(s)	1203, 1698, 2513, 2848
BstAPI	GCANNNNNTGC	1 cut(s)	668
BstBI	TTCGAA	1 cut(s)	2022
BstC8I	GCNNGC	6 cut(s)	33, 159, 863, 1047, 1133, 2645
BstDSI	CCRYGG	1 cut(s)	2200
BstEII	GGTNACC	1 cut(s)	1074
BstENI	CCTNNNNNAGG	2 cut(s)	312, 369
BstF5I	GGATG	9 cut(s)	33, 267, 393, 626, 1237, 1329, 1446, 2008, 2286
BstMAI	GTCTC	5 cut(s)	355, 1229, 2268, 2274, 2563
BstNSI	RCATGY	3 cut(s)	35, 1135, 2219
BstPI	GGTNACC	1 cut(s)	1074
BstSFI	CTRYAG	2 cut(s)	660, 2256
BstSLI	GKGCMC	5 cut(s)	238, 493, 721, 1660, 2420
BstV1I	GCAGC	9 cut(s)	674, 823, 862, 1295, 1927, 2003, 2652, 2757, 2760
BstV2I	GAAGAC	1 cut(s)	2541
BstX2I	RGATCY	4 cut(s)	2450, 2486, 2830, 2891
BstXI	CCANNNNNNTGG	2 cut(s)	1965, 2771
BstYI	RGATCY	4 cut(s)	2450, 2486, 2830, 2891
BstZ17I	GTATAC	1 cut(s)	1865
Bsu36I	CCTNAGG	1 cut(s)	1389
BsuI	GTATCC	1 cut(s)	1850
BtgI	CCRYGG	1 cut(s)	2200
BtsCI	GGATG	9 cut(s)	33, 267, 393, 626, 1237, 1329, 1446, 2008, 2286
BtsI	GCAGTG	1 cut(s)	1238
BveI	ACCTGC	3 cut(s)	247, 305, 1087
Cac8I	GCNNGC	6 cut(s)	33, 159, 863, 1047, 1133, 2645
CaiI	CAGNNNCTG	3 cut(s)	164, 668, 2426
Cfr13I	GGNCC	9 cut(s)	155, 296, 443, 503, 756, 837, 881, 1849, 2849
Csp6I	GTAC	1 cut(s)	1401
CviQI	GTAC	1 cut(s)	1401
DraIII	CACNNNGTG	1 cut(s)	797
DrdI	GACNNNNNNGTC	2 cut(s)	1243, 2465
DriI	GACNNNNNGTC	2 cut(s)	1933, 2266
DseDI	GACNNNNNNGTC	2 cut(s)	1243, 2465
EaeI	YGGCCR	6 cut(s)	65, 823, 956, 2719, 2761, 2773
Eam1104I	CTCTTC	4 cut(s)	1203, 1698, 2513, 2848
Eam1105I	GACNNNNNGTC	2 cut(s)	1933, 2266
EarI	CTCTTC	4 cut(s)	1203, 1698, 2513, 2848
Ecl136II	GAGCTC	2 cut(s)	305, 689
Eco130I	CCWWGG	2 cut(s)	58, 953
Eco24I	GRGCYC	2 cut(s)	307, 691
Eco31I	GGTCTC	1 cut(s)	355
Eco47I	GGWCC	6 cut(s)	296, 443, 503, 756, 881, 1849
Eco53kI	GAGCTC	2 cut(s)	305, 689
Eco57I	CTGAAG	1 cut(s)	1829
Eco81I	CCTNAGG	1 cut(s)	1389
Eco88I	CYCGRG	3 cut(s)	2271, 2560, 2725
Eco91I	GGTNACC	1 cut(s)	1074
EcoICRI	GAGCTC	2 cut(s)	305, 689
EcoNI	CCTNNNNNAGG	2 cut(s)	312, 369
EcoO109I	RGGNCCY	3 cut(s)	443, 756, 881
EcoO65I	GGTNACC	1 cut(s)	1074
EcoRI	GAATTC	1 cut(s)	1977
EcoT14I	CCWWGG	2 cut(s)	58, 953
EcoT22I	ATGCAT	1 cut(s)	33
EcoT38I	GRGCYC	2 cut(s)	307, 691
ErhI	CCWWGG	2 cut(s)	58, 953
Esp3I	CGTCTC	1 cut(s)	2274
FaqI	GGGAC	6 cut(s)	429, 742, 1118, 1278, 2027, 2211
FauI	CCCGC	3 cut(s)	870, 871, 910
FbaI	TGATCA	2 cut(s)	1114, 2167
FblI	GTMKAC	3 cut(s)	1011, 1864, 2259
FokI	GGATG	9 cut(s)	40, 274, 400, 613, 1244, 1336, 1453, 2015, 2273
FriOI	GRGCYC	2 cut(s)	307, 691
FspBI	CTAG	7 cut(s)	396, 711, 768, 1925, 2240, 2547, 2709
GsuI	CTGGAG	3 cut(s)	540, 1059, 1090
HapII	CCGG	1 cut(s)	2595
HincII	GTYRAC	5 cut(s)	564, 699, 1259, 1743, 2470
HindII	GTYRAC	5 cut(s)	564, 699, 1259, 1743, 2470
HpaI	GTTAAC	1 cut(s)	1743
HpaII	CCGG	1 cut(s)	2595
HphI	GGTGA	5 cut(s)	659, 891, 2177, 2188, 2216
HpyCH4III	ACNGT	7 cut(s)	100, 583, 849, 1405, 1517, 2204, 2654
HpyCH4IV	ACGT	3 cut(s)	1177, 1760, 1906
HpySE526I	ACGT	3 cut(s)	1177, 1760, 1906
KflI	GGGWCCC	2 cut(s)	443, 756
Ksp22I	TGATCA	2 cut(s)	1114, 2167
KspAI	GTTAAC	1 cut(s)	1743
LguI	GCTCTTC	2 cut(s)	2513, 2848
LmnI	GCTCC	9 cut(s)	184, 247, 302, 310, 686, 694, 1816, 1882, 1988
Lsp1109I	GCAGC	9 cut(s)	674, 823, 862, 1295, 1927, 2003, 2652, 2757, 2760
LweI	GCATC	9 cut(s)	18, 252, 378, 635, 905, 1314, 1332, 1742, 1804
MaeI	CTAG	7 cut(s)	396, 711, 768, 1925, 2240, 2547, 2709
MaeII	ACGT	3 cut(s)	1177, 1760, 1906
MaeIII	GTNAC	5 cut(s)	94, 1074, 1178, 2128, 2860
MfeI	CAATTG	1 cut(s)	1803
MflI	RGATCY	4 cut(s)	2450, 2486, 2830, 2891
MhlI	GDGCHC	7 cut(s)	238, 307, 493, 691, 721, 1660, 2420
MlsI	TGGCCA	5 cut(s)	825, 958, 2721, 2763, 2775
MluNI	TGGCCA	5 cut(s)	825, 958, 2721, 2763, 2775
MlyI	GAGTC	6 cut(s)	584, 927, 1300, 1553, 2531, 2881
MmeI	TCCRAC	4 cut(s)	895, 901, 1209, 1982
Mox20I	TGGCCA	5 cut(s)	825, 958, 2721, 2763, 2775
Mph1103I	ATGCAT	1 cut(s)	33
MroXI	GAANNNNTTC	2 cut(s)	1605, 2909
MscI	TGGCCA	5 cut(s)	825, 958, 2721, 2763, 2775
MseI	TTAA	5 cut(s)	122, 1637, 1742, 2397, 2856
MslI	CAYNNNNRTG	3 cut(s)	1592, 2175, 2181
Msp20I	TGGCCA	5 cut(s)	825, 958, 2721, 2763, 2775
MspA1I	CMGCKG	4 cut(s)	835, 875, 1940, 1994
MspI	CCGG	1 cut(s)	2595
MunI	CAATTG	1 cut(s)	1803
Mva1269I	GAATGC	2 cut(s)	800, 2911
NciI	CCSGG	1 cut(s)	2596
NmuCI	GTSAC	2 cut(s)	94, 2860
NsiI	ATGCAT	1 cut(s)	33
NspI	RCATGY	3 cut(s)	35, 1135, 2219
NspV	TTCGAA	1 cut(s)	2022
PaeI	GCATGC	2 cut(s)	35, 1135
PaeR7I	CTCGAG	3 cut(s)	2271, 2560, 2725
PaqCI	CACCTGC	2 cut(s)	247, 1087
PasI	CCCWGGG	1 cut(s)	383
PciI	ACATGT	1 cut(s)	2215
PciSI	GCTCTTC	2 cut(s)	2513, 2848
PctI	GAATGC	2 cut(s)	800, 2911
PdmI	GAANNNNTTC	2 cut(s)	1605, 2909
PleI	GAGTC	6 cut(s)	584, 926, 1299, 1553, 2531, 2880
PpsI	GAGTC	6 cut(s)	584, 926, 1299, 1553, 2531, 2880
PpuMI	RGGWCCY	3 cut(s)	443, 756, 881
PscI	ACATGT	1 cut(s)	2215
PsiI	TTATAA	1 cut(s)	78
Psp124BI	GAGCTC	2 cut(s)	307, 691
Psp1406I	AACGTT	2 cut(s)	1177, 1906
Psp5II	RGGWCCY	3 cut(s)	443, 756, 881
PspEI	GGTNACC	1 cut(s)	1074
PspPI	GGNCC	9 cut(s)	155, 296, 443, 503, 756, 837, 881, 1849, 2849
PspPPI	RGGWCCY	3 cut(s)	443, 756, 881
PspXI	VCTCGAGB	1 cut(s)	2725
PstI	CTGCAG	1 cut(s)	664
PstNI	CAGNNNCTG	3 cut(s)	164, 668, 2426
PsuI	RGATCY	4 cut(s)	2450, 2486, 2830, 2891
PvuII	CAGCTG	3 cut(s)	875, 1940, 1994
RsaI	GTAC	1 cut(s)	1402
RsaNI	GTAC	1 cut(s)	1401
RseI	CAYNNNNRTG	3 cut(s)	1592, 2175, 2181
SacI	GAGCTC	2 cut(s)	307, 691
SapI	GCTCTTC	2 cut(s)	2513, 2848
SaqAI	TTAA	5 cut(s)	122, 1637, 1742, 2397, 2856
Sau96I	GGNCC	9 cut(s)	155, 296, 443, 503, 756, 837, 881, 1849, 2849
SchI	GAGTC	6 cut(s)	584, 927, 1300, 1553, 2531, 2881
SduI	GDGCHC	7 cut(s)	238, 307, 493, 691, 721, 1660, 2420
SfaNI	GCATC	9 cut(s)	18, 252, 378, 635, 905, 1314, 1332, 1742, 1804
SfcI	CTRYAG	2 cut(s)	660, 2256
Sfr274I	CTCGAG	3 cut(s)	2271, 2560, 2725
SfuI	TTCGAA	1 cut(s)	2022
SinI	GGWCC	6 cut(s)	296, 443, 503, 756, 881, 1849
SlaI	CTCGAG	3 cut(s)	2271, 2560, 2725
SmiMI	CAYNNNNRTG	3 cut(s)	1592, 2175, 2181
SpeI	ACTAGT	1 cut(s)	395
SphI	GCATGC	2 cut(s)	35, 1135
SsiI	CCGC	7 cut(s)	35, 144, 835, 863, 878, 903, 2505
SspMI	CTAG	7 cut(s)	396, 711, 768, 1925, 2240, 2547, 2709
SstI	GAGCTC	2 cut(s)	307, 691
StyI	CCWWGG	2 cut(s)	58, 953
TaaI	ACNGT	7 cut(s)	100, 583, 849, 1405, 1517, 2204, 2654
TaiI	ACGT	3 cut(s)	1180, 1763, 1909
TaqI	TCGA	7 cut(s)	2022, 2272, 2345, 2561, 2726, 2787, 2820
TauI	GCSGC	2 cut(s)	146, 838
Tru1I	TTAA	5 cut(s)	122, 1637, 1742, 2397, 2856
Tru9I	TTAA	5 cut(s)	122, 1637, 1742, 2397, 2856
TseFI	GTSAC	2 cut(s)	94, 2860
Tsp45I	GTSAC	2 cut(s)	94, 2860
TspGWI	ACGGA	4 cut(s)	2001, 2343, 2374, 2728
VneI	GTGCAC	3 cut(s)	234, 489, 717
VpaK11BI	GGWCC	6 cut(s)	296, 443, 503, 756, 881, 1849
XagI	CCTNNNNNAGG	2 cut(s)	312, 369
XapI	RAATTY	7 cut(s)	71, 171, 940, 1423, 1977, 2148, 2302
XceI	RCATGY	3 cut(s)	35, 1135, 2219
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1304, 1469
XhoI	CTCGAG	3 cut(s)	2271, 2560, 2725
XmiI	GTMKAC	3 cut(s)	1011, 1864, 2259
XmnI	GAANNNNTTC	2 cut(s)	1605, 2909
XspI	CTAG	7 cut(s)	396, 711, 768, 1925, 2240, 2547, 2709
Zsp2I	ATGCAT	1 cut(s)	33

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.